1. Role of RpoS in stress resistance, biofilm formation and quorum sensing of Shewanella baltica
- Author
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Zhang Caili, Y. Liu, Liu Haimei, C. Wang, and Abdul-Nabi Jatt
- Subjects
0106 biological sciences ,Shewanella ,Hot Temperature ,genetic processes ,Mutant ,Regulator ,Sigma Factor ,01 natural sciences ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,Bacterial Proteins ,Stress, Physiological ,010608 biotechnology ,Baltica ,Shewanella baltica ,0303 health sciences ,biology ,Ethanol ,030306 microbiology ,Chemistry ,Wild type ,Biofilm ,Quorum Sensing ,Gene Expression Regulation, Bacterial ,Hydrogen Peroxide ,biochemical phenomena, metabolism, and nutrition ,equipment and supplies ,biology.organism_classification ,carbohydrates (lipids) ,Quorum sensing ,Seafood ,Biofilms ,bacteria ,rpoS ,Locomotion - Abstract
Shewanella baltica is one of the most important bacterial species contributing to spoilage of seafood. Principally, RpoS has been recognized as the central regulator of stress resistance in many bacterial species. However, little is known about the role of RpoS in S. baltica. In this study, an rpoS mutant of S. baltica was constructed and analysed for its functions. The results showed that the survival rate of rpoS mutant decreased when treated with heat, ethanol and H2 O2, while increased the resistance to NaCl. Moreover RpoS promoted the biofilm formation of S. baltica at 30°C, while declined at 4°C. Interestingly, the rpoS-deficient mutant showed increased swimming motility. Furthermore, the results revealed that the production of quorum-sensing (QS) signals such as cyclo-(l-Pro-l-Leu) and cyclo-(l-Pro-l-Phe) reduced in rpoS mutant. Mainly, rpoS positively regulated QS response regulators, as the expression of all luxR genes in rpoS mutant significantly decreased relative to wild type. This study reveals that RpoS is a major regulator involved in stress responses, biofilm formation and quorum sensing system in S. baltica. The present work provides significant information for the control of microbiological spoilage of seafood.
- Published
- 2020