1. Analysis of astragaloside IV metabolism to cycloastragenol in human gut microorganism, bifidobacteria, and lactic acid bacteria
- Author
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Daniel M, Takeuchi, Shigenobu, Kishino, Yuuki, Ozeki, Hiroyuki, Fukami, and Jun, Ogawa
- Subjects
Sapogenins ,Xylose ,Bacteria ,Organic Chemistry ,General Medicine ,Saponins ,Applied Microbiology and Biotechnology ,Biochemistry ,Triterpenes ,Analytical Chemistry ,Glucose ,Lactobacillales ,Humans ,Bifidobacterium ,Molecular Biology ,Biotechnology - Abstract
This study investigated different gut bacteria in an anaerobic environment to identify specific candidates that could transform astragaloside IV (AIV) to cycloastragenol (CA). Two representative gut microbes, lactic acid bacteria (LAB) and bifidobacteria, could metabolize AIV to CA. Multiple screenings showed two metabolic pathways to metabolize AIV in two groups of bacteria. LAB metabolized AIV initiated by removing the C-6 glucose, whereas bifidobacteria indicated the initial removal of C-3 xylose. The final products differed between the two groups as bifidobacteria showed the production of CA, whereas LAB demonstrated preferential production of 20R, 24S-epoxy-6α, -16β, -25-trihydroxy-9, -19-cycloartan-3-one (CA-2H).
- Published
- 2022
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