57 results on '"Yun-Long, Zhao"'
Search Results
2. Effects of different stocking densities on the growth performance and antioxidant capacity of Chinese mitten crab (Eriocheir sinensis) in rice crab culture system
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Xin Wang, Qi Yao, Xin-yu Lei, Dong-ming Zhang, Sen Wang, Ji-wu Wan, Hong-jian Liu, Yu-ke Chen, Yun-long Zhao, Gui-qin Wang, Qiu-ju Wang, and Zhi-xin Guo
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Aquatic Science ,Agronomy and Crop Science - Published
- 2022
3. Impact of the COVID-19 pandemic on congenital diaphragmatic hernia patients: a single-center retrospective study
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Yun-Long, Zhao, Ying, Wang, Chao, Liu, Yu-Lin, Jiang, Yan-Dong, Wei, Hua, Meng, Shan, Jian, Xi-Ting, Zhu, Li-Jian, Pei, Xiao-Chen, Bai, Feng, Feng, Yan, Lv, Xi-Ya, Zhou, Qing-Wei, Qi, Jing-Na, Li, Wei, Ji, and Li-Shuang, Ma
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SARS-CoV-2 ,Pediatrics, Perinatology and Child Health ,COVID-19 ,Humans ,Surgery ,General Medicine ,Child ,Hernias, Diaphragmatic, Congenital ,Pandemics ,Retrospective Studies - Abstract
Purpose To investigate the impact of COVID-19 on the treatment of children with congenital diaphragmatic hernia (CDH). Methods We retrospectively collected and compared the data of patients with CDH admitted between January 1, 2020 and December 31, 2021(study group) with the CDH patients admitted before the pandemic between January 1, 2018 and December 31, 2019 (control group). Results During the pandemic, 41 patients with CDH diagnosed prenatally were transferred to our hospital, and 40 underwent surgical repair. The number of patients treated in our hospital increased by 24.2% compared with the 33 patients before the pandemic. During the pandemic, the overall survival rate, postoperative survival rate and recurrence rate were 85.4%, 87.5% and 7.3%, respectively, and there were no significant differences compared with the control group (75.8%, 83.3% and 9.1%, respectively). The average length of hospital stay in patients admitted during the pandemic was longer than that in the control group (31 days vs. 16 days, P P = 0.037). Conclusions CDH patients confirmed to be SARS-CoV-2 infection-free can receive routine treatment. Our data indicate that the implementation of protective measures during the COVID-19 pandemic, along with appropriate screening and case evaluation, do not have a negative impact on the prognosis of children.
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- 2022
4. Recommendations for perinatal and neonatal surgical management during the COVID-19 pandemic
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Li-Shuang Ma, Yandong Wei, Chao Liu, and Yun-Long Zhao
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medicine.medical_specialty ,Protection ,Medical staff ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Neonates ,COVID-19 ,General Medicine ,Recommendation ,Pediatrics ,Neonatal surgery ,Medical services ,03 medical and health sciences ,0302 clinical medicine ,Emergency surgery ,030220 oncology & carcinogenesis ,Pandemic ,Global health ,Surgical management ,Medicine ,030211 gastroenterology & hepatology ,business ,Intensive care medicine ,Expert Recommendations - Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 has spread rapidly around the world and is a significant threat to global health. Patients in the Neonatal Surgery Department have rapidly progressing diseases and immature immunity, which makes them vulnerable to pulmonary infection and a relatively higher mortality. This means that these patients require multidisciplinary treatment including early diagnosis, timely transport, emergency surgery and intensive critical care. The COVID-19 pandemic poses a threat to carrying out these treatments. To provide support for the health protection requirements of the medical services in the Neonatal Surgery Department, we developed recommendations focusing on patient transport, surgery selection and protection requirements with the aim of improving treatment strategies for patients and preventing infection in medical staff during the current COVID-19 pandemic.
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- 2020
5. Identification of the Nrf2 in the fathead minnow muscle cell line: role for a regulation in response to H2O2 induced the oxidative stress in fish cell
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Zhang Dongming, Xiu-Mei Chen, Gui-Qin Wang, Yun-Long Zhao, Sha Luo, Qiuju Wang, Ting Yu, and Zhixin Guo
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Physiology ,Aquatic Science ,medicine.disease_cause ,Biochemistry ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,Transcriptional regulation ,medicine ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,Glutathione peroxidase ,04 agricultural and veterinary sciences ,General Medicine ,Glutathione ,Cell cycle ,KEAP1 ,Cell biology ,chemistry ,Apoptosis ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Oxidative stress - Abstract
The Nrf2 (nuclear factor erythroid 2-related factor 2) plays a central role in cell protection against a wide variety of environmental stressors through the Nrf2-Keap1 (Kelch-like ECH-associated protein 1) pathway, but its involvement in modulation of antioxidant system of fish cell is still largely unexplored. The present study focused on the molecular cloning and silencing of the Nrf2 in the fathead minnow muscle cell line (FHM) in response to the oxidative stress induced by H2O2. A full-length cDNA of coding Nrf2 was cloned from FHM cells by RT-PCR and RACE approaches. The obtained cDNA covered 2578 bp with an open reading frame (1770 bp) of encoding 589 amino acids. Sequence alignment and phylogenetic analysis revealed a high degree of conservation (51–86%) among 16 fishes. Based on the cloned Nrf2 sequence, the siRNA-242 of targeting Nrf2 with the best knocking down efficiency was designed and detected. Then, the mRNA levels of Keap1, Nrf2, Maf (musculoaponeurotic fibrosarcoma oncogene), and HO-1 (haemoxygenase-1); the activities of T-SOD (total superoxide dismutase), CAT (catalase), and GSH-PX (glutathione peroxidase); the levels of GSH (glutathione) and MDA (malonaldehyde); and the cell cycle and apoptosis were analyzed to investigate the molecular responses after H2O2 exposure. These results showed a coordinated transcriptional regulation of Keap1, Maf, and HO-1 and antioxidants (T-SOD, GSH, CAT, and GSH-PX) and MDA levels after H2O2 exposure, leading to oxidative damage and apoptosis. These findings provided an insight to understand the mechanisms of Nrf2 against oxidative stress in fish.
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- 2020
6. Establishment and validation of a nomogram model for periprosthetic infection after megaprosthetic replacement around the knee following bone tumor resection: A retrospective analysis
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Hao-ran Zhang, Feng Wang, Yun-long Zhao, Xiu-chun Yu, Yong-cheng Hu, Ji-kai Li, Ming-you Xu, Rui-qi Qiao, Xiong-Gang Yang, and Pang Chenggang
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Adult ,Male ,medicine.medical_specialty ,Multivariate analysis ,Knee Joint ,Periprosthetic ,Bone Neoplasms ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Hematoma ,medicine ,Humans ,Orthopedics and Sports Medicine ,Risk factor ,Retrospective Studies ,030222 orthopedics ,Proportional hazards model ,business.industry ,Incidence (epidemiology) ,030229 sport sciences ,Nomogram ,medicine.disease ,Surgery ,Nomograms ,Female ,business ,Cohort study - Abstract
Background Due to the particularity of patients with bone tumors, the risk of periprosthetic infection following megaprosthetic replacement is much higher than that of traditional total knee arthroplasty. Unfortunately, few studies specifically reported the risk factors for periprosthetic infection following megaprosthetic replacement. The purposes of the study were to (1) establish a nomogram model, which can provide a reference for clinicians, and patients to reduce the occurrence of periprosthetic infection (2) explore the risk factors for deep infection of megaprosthesis. Hypothesis A prediction model can be established and has favorable predictive accuracy. Patients and Methods One hundred and seventy-seven megaprostheses were identified. There were 61 female patients and 116 male patients with a mean age of 35 years. The following risk factors were analyzed: tumor site, sex, age, material for prosthetic stem, tumor type, smoking, diabetes, length of bone resection, operation time, chemotherapy, BMI, malignant tumor staging and hematoma formation. Finally, based on the multivariate analysis, the independent risk factors were used to develop a nomogram model. Results Univariate Cox regression analysis showed that the chemotherapy, longer operation time and hematoma formation were risk factors for periprosthetic infection. Multivariate Cox regression analysis showed that the chemotherapy, longer operation time and hematoma formation were significant risk factors for periprosthetic infection. The nomogram model was established using these significant risk factors, with a C-index of 0.766 and an acceptable consistency according to the internal validation, indicating that the prediction model had favorable predictive accuracy. Discussion This study has important implications for the future investigations of prevention of periprosthetic infection. The nomogram model identifies high-risk patients for whom attached prophylaxis measures are required. Future studies regarding reduction of incidence of periprosthetic infection should pay close attention to these high-risk patients. Level of evidence IV, retrospective, cohort study.
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- 2020
7. Research on Fault Diagnosis Method of Electric Submersible Plunger Pump Lifting Technology
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Kun Qian, Chun-long Sun, Qiang Li, Hong-liang Guo, Dong-zhi Zheng, Yun-long Zhao, Ji-nan Li, Feng-ying Wang, Xin Wei, Bao-zhong Zhang, Ying Zhang, and Guang-wei Chen
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- 2022
8. High-quality CdS quantum dots sensitized ZnO nanotube array films for superior photoelectrochemical performance
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Qian-Qian Gong, Yun-Long Zhao, Qi Zhang, Chun-Yong Hu, Teng-Fei Liu, Hai-Feng Zhang, Guang-Chao Yin, and Mei-Ling Sun
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General Physics and Astronomy - Abstract
The surface characteristics of ZnO were synthetically optimized by a self-designed simultaneous etching and W-doping hydrothermal method utilizing as-prepared ZnO nanorod (NR) array films as the template. Benefiting from the etching and regrowth process and the different structural stabilities of the various faces of ZnO NRs, the uniquely etched and W-doped ZnO (EWZ) nanotube (NT) array films with larger surface area, more active sites and better energy band structure were used to improve the photoelectrochemical (PEC) performance and the loading quality of CdS quantum dots (QDs). On the basis of their better surface characteristics, the CdS QDs were uniformly loaded on EWZ NT array film with a good coverage ratio and interface connection; this effectively improved the light-harvesting ability, charge transportation and separation as well as charge injection efficiency during the PEC reaction. Therefore, all the CdS QD-sensitized EWZ NT array films exhibited significantly enhanced PEC performance. The CdS/EWZ-7 composite films exhibited the optimal photocurrent density with a value of 12 mA⋅cm−2, 2.5 times higher than that of conventional CdS/ZnO-7 composite films under the same sensitization times with CdS QDs. The corresponding etching and optimizing mechanisms were also discussed.
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- 2022
9. Construction of Complex Bisether-Bridged Medium-Sized Cyclic Compounds from o-(1-(Acyloxy)propargyl)benzaldehydes under Base and Acid Catalysis
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Ying Cheng and Yun-Long Zhao
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chemistry.chemical_classification ,Ethanol ,Aqueous solution ,Base (chemistry) ,010405 organic chemistry ,Chemistry ,Allene ,Organic Chemistry ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,Catalysis ,Acid catalysis ,chemistry.chemical_compound ,Propargyl - Abstract
We report herein our serendipitous discovery of the rapid and straightforward accesses to unprecedented diverse complex molecular structures from readily available starting materials. Catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene under mild conditions, o-(1-(acyloxy)propargyl)benzaldehydes 1 underwent efficient and selective dimerization reactions to produce novel complex bisether-bridged tricyclic products 3 and 4. The reactions proceeded most probably through dimerizations between 3-methylene-3H-isochromene intermediate and its zwitterionic resonance structures which were generated from a concerted 6-π electrocyclic ring closure reaction from the initially formed (2-formylphenyl)allene intermediates derived directly from o-(1-(acyloxy)propargyl)benzaldehydes. Treatment of the resulting product simply with NaOEt in ethanol and aqueous HCl, respectively, enabled further development of complex molecular diversities.
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- 2019
10. Brachypterous Stenus species (Coleoptera, Staphylinidae, Steninae) from West-Central China
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Yun-Long Zhao, Volker Puthz, and Liang Tang
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Steninae ,biology ,Ecology ,Insect Science ,Central china ,Taxonomy (biology) ,Stenus ,China ,biology.organism_classification - Abstract
Five new brachypterous species belonging to the genus Stenus Latreille are described from Taibaishan Nature Reserve, Shaanxi Prov., West-Central China: S. (Hypostenus) hui Tang & Puthz sp. n., S. (Hemistenus) alioventralis Tang & Puthz sp. n., S. (Hemistenus) fortunatoris Tang & Puthz sp. n., S. (Hemistenus) taibaishanus Tang & Puthz sp. n. and S. (Hypostenus) nigriceps Tang & Puthz sp. n.
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- 2019
11. Effects of different stocking densities on growth performance, nutritional quality and economic benefit of juvenile female Chinese mitten crab (Eriocheir sinensis) in rice-crab culture systems
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Xin Wang, Rong-xin Zheng, Dong-ming Zhang, Xin-yu Lei, Sen Wang, Ji-wu Wan, Hong-jian Liu, Yu-ke Chen, Yun-long Zhao, Gui-qin Wang, Qiu-ju Wang, and Zhi-xin Guo
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Aquatic Science - Published
- 2022
12. Capn4 promotes esophageal squamous cell carcinoma metastasis by regulating ZEB1 through the Wnt/β-catenin signaling pathway
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Shao-Hua Zhou, Jing-Bo Li, Shaojun Li, Hui Xia, Ying-Jie Li, and Yun-Long Zhao
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0301 basic medicine ,Pulmonary and Respiratory Medicine ,business.industry ,Cell ,Wnt signaling pathway ,Cancer ,Cell migration ,General Medicine ,medicine.disease ,digestive system diseases ,Metastasis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,Downregulation and upregulation ,030220 oncology & carcinogenesis ,Cancer research ,medicine ,Gene silencing ,Signal transduction ,business - Abstract
Background Capn4 and ZEB1 play important roles in the metastasis of several types of cancer. However, the roles and relationship of Capn4 and ZEB1 in esophageal squamous cell carcinoma (ESCC) remain unclear. Methods ESCC tumor tissues and corresponding normal esophageal epithelial tissues were obtained from 86 patients undergoing resection surgery at the Department of General Surgery, First Affiliated Hospital of Chinese PLA General Hospital from 2012 to 2017. Cell migration and invasion were examined via quantitative real-time PCR and Western blot assay. Results Our results indicate that both Capn4 and ZEB1 are significantly upregulated in ESCC tissues compared to corresponding adjacent tissues, and a positive correlation between expression and associated malignant characteristics was found. Silencing of Capn4 expression markedly inhibited ESCC invasion and metastasis in vitro and in vivo, and was accompanied by decreased ZEB1 expression. Furthermore, the anti-metastasis role of Capn4 silencing was reversed by ZEB1 overexpression, whereas knockdown of ZEB1 decreased ESCC metastasis driven by the upregulation of Capn4. Mechanistically, Capn4 regulated ZEB1 expression via activation of the Wnt/β-catenin signaling pathway in ESCC cells. Conclusion Overall, our results show that enhanced Capn4 expression activates the Wnt/β-catenin signaling pathway, resulting in increased ZEB1 expression and the promotion of ESCC cell metastasis.
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- 2018
13. Effects of acute salinity stress on osmoregulation, antioxidant capacity and physiological metabolism of female Chinese mitten crabs (Eriocheir sinensis)
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Xin Wang, Qi Yao, Dong-ming Zhang, Xin-yu Lei, Sen Wang, Ji-wu Wan, Hong-jian Liu, Yu-ke Chen, Yun-long Zhao, Gui-qin Wang, Qiu-ju Wang, and Zhi-xin Guo
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Aquatic Science - Published
- 2022
14. Asymmetric Synthesis of Multifunctionalized 2,3-Benzodiazepines by a One-Pot N-heterocyclic Carbene/Chiral Palladium Sequential Catalysis
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Yun-Long Zhao, Pei Wu, Shuang-Shuo Niu, Ying Cheng, and Ya-Li Ding
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Denticity ,010405 organic chemistry ,Aryl ,Organic Chemistry ,Enantioselective synthesis ,chemistry.chemical_element ,010402 general chemistry ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,chemistry ,Enantiomer ,Carbene ,Phosphine ,Palladium - Abstract
We report the first example of the construction of chiral 2,3-benzodiazepine compounds which are of biologic and pharmaceutical relevance by asymmetric catalysis. Catalyzed by a thiazolium-derived carbene and a palladium-chiral bidentate phosphine complex in sequence, one-pot reaction between 1-(2-(2-nitrovinyl)aryl)allyl esters 1 with azodicarboxylates 2 took place efficiently at ambient temperature to produce 4-nitro-1-vinyl-1H-2,3-benzodiazepine-2,3-dicarboxylates 5 in good to excellent yields with an enantiomeric ratio of up to 95:5.
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- 2019
15. Sliding hip screw versus cannulated cancellous screws for fixation of femoral neck fracture in adults: A systematic review
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Jianxiong Ma, Fan Zhengrui, Chen Hengting, Chao Han, Xing Fei, Xinlong Ma, Lu-kai Zhang, Yun-long Zhao, and Ming-jie Kuang
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Adult ,Male ,Bone Screws ,Operative Time ,Dentistry ,Cochrane Library ,complex mixtures ,Femoral Neck Fractures ,Fracture Fixation, Internal ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,medicine ,Humans ,Effective treatment ,Aged ,Femoral neck ,030222 orthopedics ,Osteosynthesis ,business.industry ,fungi ,030208 emergency & critical care medicine ,Recovery of Function ,General Medicine ,Length of Stay ,Middle Aged ,Functional recovery ,humanities ,Treatment Outcome ,medicine.anatomical_structure ,Systematic review ,Harris Hip Score ,Female ,Surgery ,business - Abstract
Objective Femoral neck fracture is considered a difficult fracture to treat and often gives rise to unsatisfactory treatment results. Cannulated cancellous screws (CCS) or a sliding hip screw (SHS) are the mainstream internal fixations used for osteosynthesis of femoral neck fractures. There is a need to integrate existing data through a meta-analysis to investigate the safety and effectiveness of CCS and SHS in the treatment of femoral neck fractures. Method According to the Cochrane Handbook for Systematic Reviews of Interventions, we screened for the relevant studies by searching Google Scholar, the Cochrane Controlled Trials Register, the Cochrane Library, Web of Science, EMBASE, and PubMed. The PICOS criteria was used to make sure the included studies fulfilled the inclusion criteria. Results Pooled data showed that there were no significant differences between the SHS and CCS groups for the Harris Hip Score. Significant differences were found between the SHS and CCS groups in terms of union time, postoperative complications, blood loss, operation time, incision length and length of hospital stay. Conclusions Although the SHS and CCS groups showed similar functional recovery in treatment of femoral neck fracture in terms of the Harris Hip Score, the SHS group showed fewer postoperative complications and faster union time for patients with femoral neck fractures. Therefore, compared with CCS, the use of SHS may be a more effective treatment of femoral neck fractures.
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- 2018
16. Soybean and cottonseed meals are good candidates for fishmeal replacement in the diet of juvenile Macrobrachium nipponense
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Yan-Yan Cui, Liqiao Chen, Wu-Jiang Fan, Zhen-Yu Du, Dong-Liang Li, Fang Qiao, Yu-Juan Huang, Nan-Nan Zhang, Yun-Long Zhao, and Samwel Mchele Limbu
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0301 basic medicine ,Meal ,digestive, oral, and skin physiology ,Soybean meal ,food and beverages ,04 agricultural and veterinary sciences ,Aquatic Science ,Biology ,Feed conversion ratio ,Cottonseed ,03 medical and health sciences ,030104 developmental biology ,Animal science ,Fish meal ,Plant protein ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Macrobrachium nipponense ,Cottonseed meal ,Agronomy and Crop Science - Abstract
Macrobrachium nipponense is a new aquaculture species with high market demand and is preferred as food by Asian consumers. However, its nutrient requirements and optimal dietary sources have not been fully evaluated. In the present study, we examined four common plant protein sources (soybean meal, cottonseed meal, rapeseed meal, and peanut meal) as partial replacement for 25% fishmeal protein in five isonitrogenous and isocaloric diets in M. nipponense for 8 weeks. The nutritional effects of the four plant protein sources were compared by means of growth performance, feed efficiency, histology, biochemical composition, oxidative stress, total hemocyte count, and in vitro digestion. Prawns fed the diets containing soybean (730.96 ± 33.50%) and cottonseed meals (672.32 ± 74.52%) had higher weight gain than those fed on rapeseed (503.71 ± 50.85%) and peanut (507.79 ± 52.10%) meal diets (p
- Published
- 2017
17. miR-21 modulates the effect of EZH2 on the biological behavior of human lung cancer stem cells in vitro
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Yang Liu, Yingnan Zhao, Yun-Long Zhao, Hui Xia, Wen Zhang, Baoshi Zhang, and Shaojun Li
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0301 basic medicine ,medicine.medical_treatment ,macromolecular substances ,chemotherapy ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,EZH2 ,Lung cancer ,radiotherapy ,Cyclin-dependent kinase 1 ,Chemotherapy ,business.industry ,Cell cycle ,medicine.disease ,Radiation therapy ,030104 developmental biology ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Immunology ,Cancer research ,miR-21 ,lung cancer stem cells ,Stem cell ,business ,Research Paper - Abstract
// Hui Xia 1, 2, 3 , Wen Zhang 1 , Baoshi Zhang 1 , Yingnan Zhao 1 , Yunlong Zhao 1 , Shaojun Li 1 and Yang Liu 2 1 Department of Thoracic-Cardio Surgery, First Affiliated Hospital of PLA General Hospital, Beijing, China 2 Department of Thoracic Surgery, PLA General Hospital, Beijing, China 3 Medical School of PLA, Beijing, China Correspondence to: Hui Xia, email: 304xiahui@sina.com Yang Liu, email: China liuyangplats@126.com Keywords: miR-21, EZH2, lung cancer stem cells, radiotherapy, chemotherapy Received: November 10, 2016 Accepted: June 19, 2017 Published: August 07, 2017 ABSTRACT Non-small-cell lung cancer has a high mortality rate and poor prognosis. Therefore, novel therapeutic approaches are urgently needed to enhance patient survival rates. In this study, we investigated the effects of miR-21 and EZH2 on the biological behavior of human lung cancer stem cells in vitro . We found increased expression of EZH2 and miR-21 in LCSCs, and miR-21 overexpression increased EZH2 levels in LCSCs. In addition, EZH2 and miR-21 knockdown increased the sensitivity of LCSCs to chemo- and radiation therapy, and exogenous EZH2 expression rescued the effects of anti-miR-21. Cell proliferation was reduced by 39.2% and 69.7% in the presence of radio - or chemotherapy combined with anti-miR-21 transfection, respectively. The downstream molecules included Cdc2, cyclin B1, and Bcl-2, which are involved in the regulation of cell cycle and apoptosis and which could themselves be reduced or enhanced by changes in miR-21 and EZH2 levels in LCSCs. This study demonstrates the direct relationship between miR-21 and EZH2 which was increased by 43% after the application of the miR-21 mimic. Above data indicates that these two molecules can influence the biological behavior of LCSCs by altering their corresponding targets. Our findings support the potential roles of miR-21 and EZH2 in improving the therapeutic efficacy of clinical lung cancer treatments.
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- 2017
18. Polyethylene glycol-induced motor recovery after total spinal transection in rats
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Ming Li, Shuai Ren, Qiong Wu, Jun Wu, Ya Shan Guo, Yan Xue, Qing Miao, Ze Han Liu, Sheng Yu Wang, Zhen Xue, Kuang Fu, Xiao Ping Ren, Xin Zhao, Sergio Canavero, Yun Long Zhao, and Li Ting Hou
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Male ,medicine.medical_specialty ,Cord ,medicine.medical_treatment ,Motor Activity ,Polyethylene Glycols ,Random Allocation ,03 medical and health sciences ,0302 clinical medicine ,Evoked Potentials, Somatosensory ,Physiology (medical) ,Paralysis ,Animals ,Medicine ,Pharmacology (medical) ,Saline ,Spinal cord injury ,Spinal Cord Injuries ,Pharmacology ,business.industry ,Original Articles ,Recovery of Function ,Spinal cord ,medicine.disease ,Magnetic Resonance Imaging ,Rats ,Surgery ,Disease Models, Animal ,Psychiatry and Mental health ,Diffusion Tensor Imaging ,Neuroprotective Agents ,medicine.anatomical_structure ,Spinal Cord ,Somatosensory evoked potential ,030220 oncology & carcinogenesis ,Anesthesia ,Vertebrectomy ,Animal studies ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
SummaryAims Despite more than a century of research, spinal paralysis remains untreatable via biological means. A new understanding of spinal cord physiology and the introduction of membrane fusogens have provided new hope that a biological cure may soon become available. However, proof is needed from adequately powered animal studies. Methods and results Two groups of rats (n=9, study group, n=6 controls) were submitted to complete transection of the dorsal cord at T10. The animals were randomized to receive either saline or polyethylene glycol (PEG) in situ. After 4 weeks, the treated group had recovered ambulation vs none in the control group (BBB scores; P=.0145). One control died. All animals were studied with somatosensory-evoked potentials (SSEP) and diffusion tensor imaging (DTI). SSEP recovered postoperatively only in PEG-treated rats. At study end, DTI showed disappearance of the transection gap in the treated animals vs an enduring gap in controls (fractional anisotropy/FA at level: P=.0008). Conclusions We show for the first time in an adequately powered study that the paralysis attendant to a complete transection of the spinal cord can be reversed. This opens the path to a severance-reapposition cure of spinal paralysis, in which the injured segment is excised and the two stumps approximated after vertebrectomy/diskectomies.
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- 2017
19. Organocatalytic Friedel–Crafts Alkylation/Lactonization Reaction of Naphthols with 3‐Trifluoroethylidene Oxindoles: The Asymmetric Synthesis of Dihydrocoumarins
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Junling Zhao, Qinxin Lou, Yun-Long Zhao, Longsheng Wang, and Wenhui Hu
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Chemistry ,010405 organic chemistry ,Squaramide ,Enantioselective synthesis ,General Chemistry ,General Medicine ,Alkylation ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,chemistry.chemical_compound ,Nucleophile ,Amide ,Organocatalysis ,Organic chemistry ,Friedel–Crafts reaction - Abstract
Naphthols and 3-trifluoroethylidene oxindoles were found to undergo an asymmetric Friedel–Crafts alkylation/lactonization reaction, catalyzed by only 2.5 mol % of a quinine-derived squaramide catalyst, to afford the corresponding α-aryl-β-trifluoromethyl dihydrocoumarin derivatives in high yields (up to 99 %) with excellent enantio- and diastereoselectivities (up to 98 % ee, >20:1 d.r.). Importantly, the lactonization proceeded by nucleophilic attack of the naphthol hydroxy group at the amide motif of the oxindoles under mild reaction conditions. This protocol represents a new strategy for the formation of dihydrocoumarins by an efficient intramolecular amide C−N bond-cleavage and esterification process.
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- 2016
20. Construction of Complex Bisether-Bridged Medium-Sized Cyclic Compounds from
- Author
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Yun-Long, Zhao and Ying, Cheng
- Abstract
We report herein our serendipitous discovery of the rapid and straightforward accesses to unprecedented diverse complex molecular structures from readily available starting materials. Catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene under mild conditions
- Published
- 2019
21. L-carnitine regulated Nrf2/Keap1 activation in vitro and in vivo and protected oxidized fish oil-induced inflammation response by inhibiting the NF-κB signaling pathway in Rhynchocypris lagowski Dybowski
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Yu-Ke Chen, Ting Yu, Yong-sheng Gao, Yun-Long Zhao, Qiuju Wang, Zhixin Guo, Dong-Ming Zhang, Xiu-Mei Chen, and Guiqin Wang
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0301 basic medicine ,Fish Proteins ,Antioxidant ,NF-E2-Related Factor 2 ,medicine.medical_treatment ,Anti-Inflammatory Agents ,Cyprinidae ,Inflammation ,Aquatic Science ,Pharmacology ,Biology ,medicine.disease_cause ,Protective Agents ,Antioxidants ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Fish Diseases ,Random Allocation ,Fish Oils ,In vivo ,Carnitine ,medicine ,Environmental Chemistry ,Animals ,Dose-Response Relationship, Drug ,NF-kappa B ,NF-κB ,04 agricultural and veterinary sciences ,General Medicine ,Glutathione ,Fish oil ,KEAP1 ,Oxidative Stress ,030104 developmental biology ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,medicine.symptom ,Carrier Proteins ,Oxidation-Reduction ,Oxidative stress ,Signal Transduction - Abstract
Nrf2/Keap1 pathway is associated with oxidative stress. l-carnitine is currently under preclinical evaluation as a antioxidant, but the use of l-carnitine in aquaculture has been poorly evaluated and so far no mechanism has been demonstrated. Here, we explored the effects of l-carnitine in vitro and in vivo and discussed the possible molecular mechanisms involved. Firstly, Nrf2-siRNA significantly knocked down the mRNA level of Nrf2 in FHM cells. Thus, the activities of antioxidant enzymes (T-SOD, CAT, GSH-PX) and the level of antioxidant substance (GSH) and the level of MDA showed that Nrf2-siRNA pretreatment weakened the protective effect of l-carnitine. Moreover, the mRNA levels of Keap1, Nrf2, Maf and HO-1 indicated that l-carnitine regulated Nrf2/Keap1 activation. Furthermore, oxidized fish oil remarkably suppressed growth in Rhynchocypris lagowski Dybowski, and the lower antioxidant capacity was also observed in liver. According to the results of immune related indexes (the levels of IL-1β, TNF-α, LZM, AKP) in serum and the mRNA levels of immune related genes (NF-κB, IL-1β, TNF-α, IL-8, IL-10 and TGF-β) in liver, oxidized fish oil also induced inflammatory response in fish. Also, l-carnitine supplementation can relieve this bad condition. In conclusion, l-carnitine regulated Nrf2/Keap1 activation in vitro and in vivo and protected oxidized fish oil-induced inflammation response by inhibiting the NF-κB signaling pathway in Rhynchocypris lagowski Dybowski.
- Published
- 2019
22. Role of the Janus kinase 2/signal transducers and activators of transcription 3 pathway in the protective effect of remote ischemia preconditioning against cerebral ischemia-reperfusion injury in rats
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Xiangchen Guan, Xin Zhao, Ming Li, Yan Xue, Shuai Ren, Zehan Liu, Xiaoping Ren, Yun-Long Zhao, and Yang Song
- Subjects
0301 basic medicine ,Male ,STAT3 Transcription Factor ,Janus kinase 2/signal transducers and activators of transcription 3 signal pathway ,Ischemia ,Infarction ,Pharmacology ,Brain Ischemia ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,STAT3 ,Ischemic Preconditioning ,Janus kinase 2 ,biology ,business.industry ,Cerebral infarction ,General Neuroscience ,apoptosis ,inflammatory factor ,Janus Kinase 2 ,medicine.disease ,cerebral ischemia–reperfusion injury ,Rats ,030104 developmental biology ,Reperfusion Injury ,biology.protein ,Ischemic preconditioning ,Tumor necrosis factor alpha ,remote ischemic preconditioning ,business ,Cellular, Molecular and Developmental Neuroscience ,Reperfusion injury ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
Remote ischemia preconditioning (RIPC) is a convenient and effective method for alleviating cerebral ischemia-reperfusion injury (CIRI). However, to date, the underlying mechanism has not been fully elucidated. The aim of this research was to explore the protective mechanism of RIPC on the brain after CIRI. Four groups of rats were included in this experiment: the sham group, the middle cerebral artery occlusion (MCAO) group, the RIPC group, and the AG490 group. As an inhibitor of Janus kinase 2 (JAK2), AG490 was used after MCAO in the AG490 group to explore the role of JAK2/signal transducers and activators of transcription 3 (STAT3) after CIRI. Brain tissue was collected for evaluation after 2 h of ischemia and 24 h of reperfusion. ELISA for interleukin (IL)-6, IL-1β and tumor necrosis factor-α, western blot for phosphorylated-JAK2 and phosphorylated-STAT3, the neurological severity score and Longa scoring system for neurological deficit evaluation, triphenyltetrazolium chloride staining for cerebral infarction, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining for apoptotic cells in the brain tissue were performed. Neurological function in the RIPC group was notably better than that in the MCAO group. There were smaller infarction sizes and fewer apoptotic cells in the ischemic area in the RIPC group than in the MCAO group. In the RIPC group, the expression levels of IL-1β, tumor necrosis factor-α, IL-6, and phosphorylated-JAK2 and phosphorylated-STAT3 were significantly lower than those in the MCAO group. The findings in the RIPC and AG490 groups were similar. The inflammatory response and apoptosis are two important processes involved in brain dysfunction after CIRI. The JAK2/STAT3 signaling pathway has an underlying relationship with these two processes. These findings suggest that RIPC can alleviate the damage to brain tissue by CIRI by regulating the JAK2/STAT3 signaling pathway negatively.
- Published
- 2019
23. Inhibition of metabotropic glutamate receptor 1 suppresses tumor growth and angiogenesis in experimental non-small cell lung cancer
- Author
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Yang Liu, Chang-Hai Yu, Yun-Long Zhao, Hui Xia, and Ying-Nan Zhao
- Subjects
0301 basic medicine ,Lung Neoplasms ,Angiogenesis ,Naphthalenes ,Biology ,Receptors, Metabotropic Glutamate ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Phosphorylation ,Cell Proliferation ,Insulin-like growth factor 1 receptor ,Pharmacology ,A549 cell ,Neovascularization, Pathologic ,Cell growth ,Xenograft Model Antitumor Assays ,Squamous carcinoma ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Metabotropic glutamate receptor 1 ,Female ,Signal transduction ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Metabotropic glutamate receptor 1 (mGlu1 receptor) is expressed in many cancer cell types as compared to normal counterparts underscoring its potential role in tumor behavior. The aim of present study was to test the role of mGlu1 receptor in experimental non-small cell lung cancer (NSCLC). First, protein expression of mGlu1 receptor was higher in human NSCLC cell lines, including both adenocarcinoma and squamous carcinoma subtypes, when compared to normal bronchial epithelial cells. Inhibition of mGlu1 receptor by BAY36-7620 (an mGlu1 receptor-specific inhibitor) inhibited tumor growth and prolonged survival of mice with tumors of A549 or H1299. Treatment with BAY36-7620 suppressed AKT phosphorylation in A549 tumors and pre-treatment with BAY36-7620 blocked the L-quisqualate (a potent mGlu1 receptor agonist)-induced AKT phosphorylation in A549 cells. Treatment with BAY36-7620 reduced cellular proliferation of A549 cells. Treatment with BAY36-7620 enhanced cleaved PARP levels and reduced protein expression of bcl-2, HIF-1α, and VEGF. In contrast, treatment with L-quisqualate reduced cleaved PARP levels and enhanced protein expression of bcl-2, HIF-1α, VEGF, and IL-8, which was reversed by co-incubation with MK2206 (an AKT inhibitor). Pre-treatment with BAY36-7620 blocked the VEGF-induced AKT phosphorylation in HUVECs. Treatment of HUVECs with L-quisqualate resulted in enhancement of capillary tube formation, which was reversed by co-incubation with MK2206. Furthermore, mGlu1 receptor knockdown suppressed tumor growth and prolonged survival of mice with tumors of A549 or H1299. Collectively, inhibition of mGlu1 receptor suppressed tumor growth and angiogenesis in experimental NSCLC.
- Published
- 2016
24. The effect of oxidized fish oil on lipid metabolism in Rhynchocypris lagowski Dybowski
- Author
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Dong-Ming Zhang, Ghonimy Abdallah, Qiuju Wang, Yu-ke Chen, Xiu-Mei Chen, Zhixin Guo, Ting Yu, and Yun-Long Zhao
- Subjects
Aquatic Science ,lcsh:Aquaculture. Fisheries. Angling ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Food science ,Carnitine ,Beta oxidation ,Fatty acid synthesis ,030304 developmental biology ,lcsh:SH1-691 ,chemistry.chemical_classification ,0303 health sciences ,biology ,Oxidized fish oil ,Fatty acid ,Lipid metabolism ,04 agricultural and veterinary sciences ,biology.organism_classification ,Fish oil ,Rhynchocypris lagowski Dybowski ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,lipids (amino acids, peptides, and proteins) ,Animal Science and Zoology ,Hepatopancreas ,Fatty acid composition ,Polyunsaturated fatty acid ,medicine.drug - Abstract
Fish oil can not only provide energy for the growth and development of fish, but also constitute a variety of tissue and cellular structural components of fish. However, fish oil is easily oxidized in the process of feed processing, treatment and storage, which reduces the feed quality and threatens the health of fish. Therefore, the purpose of this study was to investigate the effects of different degrees of oxidation of fish oil in feed on fatty acid composition and lipid metabolism in hepatopancreas of Rhynchocypris lagowski Dybowski. Five diets containing five oxidation levels (0, 100, 200, 300 and 400 meq/kg) of fish oil were formulated and fed to 525 healthy R. lagowski (4.48 ± 0.14 g) that were randomly divided into 15 glass aquariums. After 8 weeks, hepatopancreas samples were taken for fatty acid composition, and biochemical analysis. The results showed that the content of Polyunsaturated fatty acid (PUFA) in muscle of R. lagowski increased significantly with the increase of oxidation degree of fish oil (P < 0.05), but there were no changes in Saturated fatty acid (SFA), Monounsaturated fatty acid (MUFA), EPA (C20:5n3), and ∑n-6 (P>0.05). Compared with the control group, the contents of SFA, MUFA and EPA in hepatopancreas were significantly increased (P < 0.05), while DHA (C22:6n3), ∑n-3, ∑n-6, and PUFA were decreased (P < 0.05). Feeding with OFO increased hepatopancreas total cholesterol (TC), triacylglycerol (TG), HDL/LDL ratio, FAS, and ACCα that involved in lipid synthesis enzymes (P < 0.05), while reduced liver esterase (HL), fatty acyl-CoA synthetase (ACS), carnitine palmitoyltransferase (CPT-1) and hormone-sensitive triglyceride lipase α (HSLα) enzyme activity and gene expression that associated with lipid decomposition (P < 0.05). It is suggested that the adverse effects of dietary oxidized oil may be achieved by promoting fatty acid synthesis, inhibiting fatty acid oxidation, increasing fatty acid uptake by hepatopancreas and reducing the ability of TG to transport from hepatopancreas to extrahepatic tissues, thus inducing fat accumulation in hepatopancreatic cells and further affecting lipid metabolism in hepatopancreas.
- Published
- 2020
25. Établissement et validation d’un modèle de facteurs de risque d’infection d’une mégaprothèse autour du genou après résection d’une tumeur osseuse
- Author
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Yong-cheng Hu, Yun-long Zhao, Ji-kai Li, Xiong-Gang Yang, Pang Chenggang, Xiu-chun Yu, Feng Wang, Hao-ran Zhang, Rui-qi Qiao, and Ming-you Xu
- Subjects
medicine.medical_specialty ,Chemotherapy ,Multivariate analysis ,business.industry ,Proportional hazards model ,Incidence (epidemiology) ,medicine.medical_treatment ,Periprosthetic ,Nomogram ,medicine.disease ,Surgery ,Hematoma ,medicine ,Orthopedics and Sports Medicine ,business ,Cohort study - Abstract
Background Due to the particularity of patients with bone tumors, the risk of periprosthetic infection following megaprosthetic replacement is much higher than that of traditional total knee arthroplasty. Unfortunately, few studies specifically reported the risk factors for periprosthetic infection following megaprosthetic replacement. The purposes of the study were to (1) establish a nomogram model which can provide a reference for clinicians and patients to reduce the occurrence of periprosthetic infection; (2) explore the risk factors for deep infection of megaprosthesis. Hypothesis A prediction model can be established and has favorable predictive accuracy. Patients and methods One hundred and seventy-seven megaprostheses were identified. There were 61 female patients and 116 male patients with a mean age of 35 years. The following risk factors were analyzed: tumor site, sex, age, material for prosthetic stem, tumor type, smoking, diabetes, length of bone resection, operation time, chemotherapy, BMI, malignant tumor staging and hematoma formation. Finally, based on the multivariate analysis, the independent risk factors were used to develop a nomogram model. Results Univariate Cox regression analysis showed that the chemotherapy, longer operation time and hematoma formation were risk factors for periprosthetic infection. Multivariate Cox regression analysis showed that the chemotherapy, longer operation time and hematoma formation were significant risk factors for periprosthetic infection. The nomogram model was established using these significant risk factors, with a C-index of 0.766 and an acceptable consistency according to the internal validation, indicating that the prediction model had favorable predictive accuracy. Discussion This study has important implications for the future investigations of prevention of periprosthetic infection. The nomogram model identifies high-risk patients for whom attached prophylaxis measures are required. Future studies regarding reduction of incidence of periprosthetic infection should pay close attention to these high-risk patients. Level of evidence IV, retrospective, cohort study.
- Published
- 2020
26. Experimental study on ammonia adsorption by coal ashes
- Author
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Kefa Cen, Junhu Zhou, Yun-long Zhao, Zhihua Wang, Weijuan Yang, and Jianzhong Liu
- Subjects
Flue gas ,Chemistry ,business.industry ,Inorganic chemistry ,Anthracite ,Selective catalytic reduction ,Ammonia ,chemistry.chemical_compound ,Adsorption ,Desorption ,Fly ash ,Environmental chemistry ,Coal ,business - Abstract
Unreacted ammonia in Selective Catalytic Reduction (SCR) and Selective Non-catalytic Reduction (SNCR) technology may be adsorbed by fly ash and lead to ash blocking in the air preheater or hindering the secondary utilization of ash. Ammonia adsorption by fly ash may have a close relationship to the concentration of ammonia in flue gas. Regarding Ximeng lignite and Yangquan anthracite as the subjects investigated, NH 3 temperature-programmed desorption (NH 3 -TPD) was utilized to study the relationship between ammonia chemical adsorption and mineral composition of the ash, the atmosphere of ash production, cooling rate of ash as well as the amount of residual carbon in the ash. The results show that rapid cooling and reduction atmosphere could increase the amount of amorphous phase in the ash and facilitate ammonia adsorption. On the other hand, ash with residual carbon could adsorb more ammonia than mineral ash as a result of acidic functional group on the surface of residual carbon.
- Published
- 2015
27. miR-218 suppresses epithelial-to-mesenchymal transition by targeting Robo1 and Ecop in lung adenocarcinoma cells
- Author
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Ping Chen, Yun-Long Zhao, Ying-Jie Li, Wen Zhang, Jie Li, Hui Xia, and Bao-shi Zhang
- Subjects
0301 basic medicine ,Cancer Research ,Epithelial-Mesenchymal Transition ,Lung Neoplasms ,Gene Expression ,Adenocarcinoma of Lung ,Nerve Tissue Proteins ,Adenocarcinoma ,Human lung ,03 medical and health sciences ,Mice ,0302 clinical medicine ,ROBO1 ,Cell Movement ,Genes, Reporter ,Cell Line, Tumor ,Medicine ,Animals ,Humans ,Epithelial–mesenchymal transition ,Receptors, Immunologic ,Lung ,Transition (genetics) ,business.industry ,General Medicine ,medicine.disease ,Xenograft Model Antitumor Assays ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Female ,RNA Interference ,Target gene ,business ,Function (biology) ,Transcription Factors - Abstract
Aim: Although, miR-218 has been implicated in epithelial-to-mesenchymal transition process, the detailed mechanisms of miR-218 involvement in epithelial-to-mesenchymal transition in human lung adenocarcinoma cell are still unclear. Materials & methods: miR-218 function assays and its target gene analysis were performed. Results: miR-218 suppresses human lung adenocarcinoma cell migration and invasion and inhibits its target gene, Ecop and Robo1 expression, which subsequently suppresses NF-κB activity and its downstream targets. Conclusion: miR-218 inhibits human lung adenocarcinoma cell migration and invasion via the suppression of Ecop and Robo1 expression, thus suggesting that miR-218 could serve as a potential therapeutic target.
- Published
- 2017
28. Spectral radiance measurement of hypersonic combustion flow based on fiber spectrometer
- Author
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Yun-long Zhao, Yijie Pan, Zhang Lisong, Zhao Ling, Wei Dong, Zhang Kai, and Qi Bin
- Subjects
Hypersonic speed ,Materials science ,Spectrometer ,business.industry ,System of measurement ,Combustion ,Temperature measurement ,Physics::Fluid Dynamics ,Optics ,Radiance ,Transmittance ,Physics::Chemical Physics ,business ,Physics::Atmospheric and Oceanic Physics ,Wind tunnel - Abstract
A high speed, high resolution spectrum measurement system based on fiber-spectrometer was built to diagnose the high temperature hypersonic combustion flow. The theoretical models of the spectral radiance and transmittance of the hypersonic combustion flow were established in this paper. The spectral radiance and transmittance of the hypersonic combustion flow were measured at the combustion wind tunnel of BISLMV. Preliminary experiments showed that the spectral radiance behaved not like typical gas radiation but have a continuous spectrum, and the spectral transmittance of the combustion gas flow was about 0.9 in the spectral range 500-900nm without strong spectral selection. The results obtained in this paper will benefit the surface temperature measurement of the thermal protection materials of space vehicle tested at BISLMV.
- Published
- 2017
29. Radiation temperature measurement of optical thick hypersonic combustion flow
- Author
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Qi Bin, Yun-long Zhao, Yijie Pan, Zhao Ling, Tian Ning, Wei Dong, and Zhang Lisong
- Subjects
Hypersonic speed ,Materials science ,Spectrometer ,business.industry ,System of measurement ,Analytical chemistry ,Radiation ,Combustion ,Physics::Fluid Dynamics ,Optics ,Radiance ,Emissivity ,Physics::Chemical Physics ,business ,Physics::Atmospheric and Oceanic Physics ,Wind tunnel - Abstract
Temperature is a critical parameter to diagnose the high temperature hypersonic combustion flow. A fiber-spectrometer based measurement system and the correlated theoretical models to measure the radiation temperature of the “optical thick” hypersonic combustion flow were established in this paper. The spectral responsivity of the fiber-spectrometer based measurement system was measured at NIM in lab condition, and the spectral radiance and emissivity of the hypersonic combustion flow were measured in-situ at the combustion wind tunnel of BISLMV. Preliminary experimental results showed the spectral emissivity of the combustion flow was about 0.1 in the spectral range 500-800nm without strong spectral selection, which resulted in the radiation temperature about 2200 K, with relative standard uncertainty about 10%.
- Published
- 2017
30. A cross-circulated bicephalic model of head transplantation
- Author
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Bing-Jian Wang, Xiaoping Ren, Zi-Long Shen, Yang Song, Sergio Canavero, Hai Jin, Xin Zhao, Yun-Long Zhao, Peng-Wei Li, and Hong-Jun Jiang
- Subjects
Male ,medicine.medical_specialty ,Ischemia ,Peristaltic pump ,Anastomosis ,03 medical and health sciences ,Electrocardiography ,0302 clinical medicine ,Axillary artery ,Physiology (medical) ,medicine.artery ,medicine ,Thoracic aorta ,Animals ,Transplantation, Homologous ,Pharmacology (medical) ,Rats, Wistar ,Vein ,Pharmacology ,Transplantation ,business.industry ,Electroencephalography ,Original Articles ,medicine.disease ,Surgery ,Rats ,Oxygen ,Psychiatry and Mental health ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Anesthesia ,Cross Circulation ,Models, Animal ,business ,Reperfusion injury ,Head ,030217 neurology & neurosurgery - Abstract
SummaryAims A successful cephalosomatic anastomosis (“head transplant”) requires, among others, the ability to control long-term immune rejection and avoidance of ischemic events during the head transference phase. We developed a bicephalic model of head transplantation to study these aspects. Methods and Results The thoracic aorta and superior vena cava of a donor rat were anastomosed with the carotid artery and extracorporeal veins of a recipient rat by vascular grafts. Before thoracotomy in the donor rat, the axillary artery and vein of the donor were connected to the carotid and the extracranial vein of the third rat through a silicone tube. The silicone tube was passed through a peristaltic pump to ensure donor brain tissue blood supply. There is no ischemia reperfusion injury in donor brain tissue analyzed by electroencephalogram. Postoperative donor has pain reflex and corneal reflex. Conclusions Peristaltic pump application can guarantee the blood supply of donor brain tissue per unit time, while the application of temperature change device to the silicone tube can protect the brain tissue hypothermia, postoperative experimental data show that there is no brain tissue ischemia during the whole operation. The application of vascular grafting can also provide the possibility of long-term survival of the model.
- Published
- 2017
31. Au-Pd Alloy Octapods with High Electrocatalytic Activity for the Oxidation of Formic Acid
- Author
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Jianliu Huang, Xiaojun Wu, Youcheng Wang, Qi-Yu Liu, Hongyan Guo, Jie Zeng, Yun-Long Zhao, and Liangbing Wang
- Subjects
Materials science ,Formic acid ,Metallurgy ,Alloy ,chemistry.chemical_element ,General Chemistry ,engineering.material ,Condensed Matter Physics ,chemistry.chemical_compound ,chemistry ,Nanocrystal ,engineering ,General Materials Science ,Nuclear chemistry ,Palladium - Published
- 2014
32. Effects of dietary oxidized fish oil on growth performance and antioxidant defense mechanism of juvenile Rhynchocypris lagowski Dybowski
- Author
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Dong-Ming Zhang, Yong-sheng Gao, Qiuju Wang, Yu-Ke Chen, Xiu-Mei Chen, Guiqin Wang, Yun-Long Zhao, Zhixin Guo, and Ting Yu
- Subjects
chemistry.chemical_classification ,0303 health sciences ,Antioxidant ,medicine.medical_treatment ,04 agricultural and veterinary sciences ,Glutathione ,Aquatic Science ,Biology ,Fish oil ,Feed conversion ratio ,KEAP1 ,03 medical and health sciences ,chemistry.chemical_compound ,Enzyme ,chemistry ,040102 fisheries ,medicine ,0401 agriculture, forestry, and fisheries ,Juvenile ,Food science ,medicine.symptom ,Weight gain ,030304 developmental biology - Abstract
Five isonitrogenous and isolipidic diets containing fresh fish oil and four degrees of oxidized fish oil (POV:100; 200; 300; 400) were formulated to investigate the effects of dietary oxidized fish oil on growth performance, the antioxidation function of Rhynchocypris lagowski Dybowski. After an 8-week feeding trial, fish fed oxidized oil diets obtained significantly lower weight gain and specific growth rate due to their lower feed efficiency and protein efficiency. With the increasing of fish oil oxidation level, MDA level of the liver significantly increased, the antioxidant enzymes (T-SOD, CAT, GSH-PX) activities and GSH content of the liver were significantly decreased, and CAT, CuZn-SOD and GSH-PX mRNA levels of the liver showed the same change trend. Further analysis showed that, Keap1 mRNA level of the liver was increased, and the Nrf2, Maf, HO-1 mRNA levels of the liver were significantly decreased. However, some parameters showed a reverse trend after the 4-week feeding. In conclusion, by checking indicators of growth performance, the main antioxidant parameters, the related genes mRNA levels, the present study showed that dietary oxidized fish oil can induce oxidative damage in R. lagowski Dybowski.
- Published
- 2019
33. Investigation of the Antibiotics in Animal Manure and Agricultural Soils in Shenyang and Surrounding Cities
- Author
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Li Na Sun, Lan Shu Jin, Su Chen, Lei Chao, and Yun Long Zhao
- Subjects
Chlortetracycline ,Soil test ,medicine.drug_class ,business.industry ,animal diseases ,Antibiotics ,Environmental engineering ,General Medicine ,Oxytetracycline ,Manure ,Agronomy ,Agriculture ,parasitic diseases ,Soil water ,medicine ,Environmental science ,Livestock ,business ,medicine.drug - Abstract
In this study, oxytetracycline, chlortetracycline and roxithromycin examples, general family of farmers livestock and poultry breeding point scale livestock and poultry breeding plant in Shenyang, Tieling and Fushun representative soil samples and the application of livestock manure are sampled and analyzed, in order to understand the situation of the antibiotic residues in manure in these regions and application of antibiotic residues in soil containing antibiotics in livestock and poultry manure.
- Published
- 2013
34. The Research Progress of Biosyufactants on the Degradation of Antibiotics during the Compost Process
- Author
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Li Na Sun, Lan Shu Jin, Su Chen, Lei Chao, and Yun Long Zhao
- Subjects
Waste management ,Compost ,medicine.drug_class ,fungi ,Antibiotics ,General Medicine ,Common method ,engineering.material ,Pulp and paper industry ,complex mixtures ,Manure ,engineering ,medicine ,Degradation (geology) ,Environmental science ,Poultry manure - Abstract
In recent years, the environmental impact of antibiotic pollution from animal manure has gradually become the focus of attention. The compost is a common method for degradation of antibiotics, the rapid degradation of antibiotics can be achieved by adjusting the different compost conditions. Biosurfactants caused all the attention as a new class of surfactants, biosurfactants used in compost will have an impact on the degradation of antibiotics in livestock and poultry manure.
- Published
- 2013
35. Evaluation of effective carrier system and function on hydrocarbon accumulation in Gaoyou Sag, Subei Basin, China
- Author
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Xian-zhi Gao, Yun-long Zhao, Ming Zhang, Hao Li, and Xiao-yan Meng
- Subjects
chemistry.chemical_classification ,Carrier system ,Petroleum engineering ,Metals and Alloys ,General Engineering ,Fault plane ,Structural basin ,Permeability (earth sciences) ,Hydrocarbon ,chemistry ,Source rock ,Sedimentary rock ,Petrology ,Porosity ,Geology - Abstract
Effective carrier system comprises carrier beds which transport hydrocarbons. The spatial and temporal effectiveness of carrier system is identified according to the relevance of hydrocarbon show, hydrocarbon inclusion and sealing ability of fault to hydrocarbons distribution, together with matching relation of activity history of fault and hydrocarbon generation history of source rock. On the basis of the above considerations, transporting ability of effective carrier system can be evaluated using parameters such as fluid potential, porosity and permeability, spatial coefficient of effective pathway as well as activity rate of fault. Additionally, a new concept of “transporting threshold porosity” was proposed. Five styles of effective carrier systems were established in Gaoyou Sag, displaying either layered or zonal distribution characteristics, and transporting time ranges from the sedimentary time of E2d2 to early stage of sanduo uplift. Effective carrier systems can be described to be lowly-efficient and highly-efficient. Major faults (convex or steep fault plane) with activity rate greater than 20 m/Ma and structure ridges of sand layers with spatial coefficient of effective pathway greater than 25% are defined to be highly-efficient carrier beds. Hydrocarbons are concentrated around high-efficient carrier beds and E1f traps of northern shanian area are predicted to have great potential.
- Published
- 2013
36. The Study on Road Performance and Pavement Structure Analysis for the Base Layer Material Stabilized by SRX
- Author
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Yue Zhang, Yun Long Zhao, and Bao Yang Yu
- Subjects
Materials science ,Structure analysis ,business.industry ,General Engineering ,Base (geometry) ,Resilient modulus ,Structural engineering ,engineering.material ,Multiple layer ,Asphalt pavement ,Crushed stone ,engineering ,Composite material ,business ,Layer (electronics) - Abstract
In order to overcome the weakness of semi-rigidity base layer,the road performance of the SRX(Solution Road RomixSoilfix) stabilized base material and the mechanics response of asphalt pavement with the base layer stabilized by SRX have been studied in this paper. The CBR value and resilient modulus of SRX stabilized base material were given by indoor test. Based on the multiple layer elastic theory, both the mechanical responses of asphalt pavement structure with the SRX stabilized base and semi-rigid base were given, and according to the calculation results, the two kinds of pavement structure fatigue life were analyzed. The results have shown that the CBR value of SRX flexible material is greater than that of graded crushed stone; the SRX stable material can be used as pavement base layer, but the fatigue performance of SRX flexible base materials should be paid much attention.
- Published
- 2012
37. Histological and Ultrastructural Studies on the Male Reproductive System and Spermatogenesis in the Red Claw Crayfish, Cherax quadricarinatus
- Author
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Yu-Jie Fan, Yun-Long Zhao, Xian-Long Weng, Chuan-Guang An, and Yong-Zhen Xu
- Subjects
medicine.medical_specialty ,media_common.quotation_subject ,Aquatic Science ,Biology ,biology.organism_classification ,Crayfish ,Andrology ,medicine.anatomical_structure ,Endocrinology ,Acinus ,Meiosis ,Internal medicine ,Cherax quadricarinatus ,medicine ,Ultrastructure ,Metamorphosis ,Spermatogenesis ,Germ cell ,media_common - Abstract
Relative to other economically important marine animals, there is limited knowledge of the reproductive characteristics of the male Australian red claw crayfish Cherax quadricarinatus. In this study, the structure of the male reproductive system of C. quadricarinatus and the progression of spermatogenesis were examined histologically and using transmission electron microscopy (TEM). The male reproductive system of C. quadricarinatus is composed of paired testes, vasa deferentia, and genital appendixes. Each testis has a non-branched collecting duct and numerous attached seminal acini. Spermatogenesis occurs mainly in the seminal acini. Germ cell development is synchronous within each acinus, but not between different acini. Spermatids, formed after meiosis, undergo a complicated metamorphosis until the aflagellate spermatozoa are finally formed. Early in the metamorphosis, a highly convoluted membrane lamellar system is assembled in the cytoplasm as the chromatin begins to condense. The convolute...
- Published
- 2011
38. Biochemical composition and digestive enzyme activities during the embryonic development of the redclaw crayfish, Cherax quadricarinatus
- Author
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Yun-Long Zhao, Wen Luo, and Jun-Jie Yao
- Subjects
chemistry.chemical_classification ,ROUGE ,biology ,Total amino acids ,Embryogenesis ,Aquatic Science ,biology.organism_classification ,Biochemistry ,chemistry ,Cherax quadricarinatus ,Digestive enzyme ,biology.protein ,Biochemical composition ,Animal Science and Zoology ,Energy source ,Polyunsaturated fatty acid - Abstract
[The biochemical composition and the activity of five digestive enzymes of embryos were studied during embryonic development in the redclaw crayfish, Cherax quadricarinatus (Von Martens, 1868), in order to investigate the relationship between embryonic development and the utilization of yolk. Carbohydrates remained a minor constituent of the yolk as a whole. The contents of total proteins and total amino acids (TAA) were all observed to decrease. Among the essential amino acids (EAA), the contents of leucine and arginine were relatively high, while of the non-essential amino acids (NEAA), the most important components were glutamine and aspartic acid. The contents of total lipids (TL) decreased during embryonic development, and the predominant fatty acids of both neutral lipids (NL) and polar lipids (PL) were C16:0, C18:0, C18:1ω9, and C18:3ω3. The five digestive enzymes tested all showed changes in their activities. The specific activities of pepsin and trypsin gradually increased in the earlier stages of embryonic development, pepsin decreased rapidly in later stages, while trypsin still kept a high level of activity. The specific activity of amylase changed in a "V" shape and reached high values again in later stages. The specific activities of cellulase and lipase showed relatively low values. It is shown that carbohydrates may play an important role in synthesizing many specific compounds to participate in signal transmission and in forming the carapace. Proteins and lipids were the dominating construction and energy substances; proteins were primarily consumed in the early stages, as against lipids in later stages. Saturated fatty acids (SFA) (16:0 and 18:0) and monounsaturated fatty acids (MUFA) (16:1ω7 and 18:1ω9) were always used for energetic purposes and polyunsaturated fatty acids (PUFA) (20:5ω3 and 22:6ω3) were important as structural components of cell membranes and in the development of the central nervous system. The activities of the digestive enzymes were controlled by their genes and expressed sequentially during embryonic development. They hydrolyse the yolk and provide construction substances and energy sources for the formation of tissues, organs, and various systems in general. La composition biochimique et l'activite de cinq enzymes digestives d'embryons ont ete etudiees au cours du developpement embryonnaire de l'ecrevisse a patte rouge, Cherax quadricarinatus (Von Martens, 1868), dans le but d'investiguer les inter-relations entre le developpement embryonnaire et l'utilisation du vitellus. Les glucides representent un constituant mineur du vitellus. Les contenus en proteines totales et en acides amines totaux (TAA) diminuent. Parmi les AA essentiels (EAA), les contenus en leucine et arginine ont ete relativement eleves, tandis que parmi les acides amines non-essentiels (NEAA), les composes les plus importants ont ete la glutamine et l'acide aspartique. Les contenus en lipides totaux (TL) diminuent au cours du developpement embryonnaire, et les acides gras predominants parmi, a la fois les lipides neutres (NL) et les lipides polaires (PL), etaient C16:0, C18:0, C18:1ω9, et C18:3ω3. Les cinq enzymes digestives testees montrent toutes un changement dans leur activite. Les activites specifiques de la pepsine et de la trypsine augmentent progressivement au cours des premiers stades du developpement embryonnaire, la pepsine decroit rapidement dans les derniers stades, tandis que la trypsine conserve un degre d'activite eleve. L'activite specifique de l'amylase change selon une forme en V et atteint a nouveau un niveau eleve dans les derniers stades. Les activites specifiques de la cellulase et de la lipase montrent des valeurs relativement basses. Il est montre que les glucides pourraient jouer un role important en synthetisant de nombreux composes specifiques pour participer au signal de transmission et a la formation de la carapace. Les proteines et les lipides ont ete les substances dominantes pour la construction et l'energie; les proteines ont ete prioritairement utilisees dans les jeunes stades et les lipides dans les derniers stades. Les acides gras satures (SFA) (16:0 et 18:0) et les acides gras mono-unsatures (MUFA) (16:1ω7 et 18:1ω9) ont toujours ete utilises pour les besoins energetiques, et les acides gras poly-unsatures (PUFA) (20:5ω3 et 22:6ω3) ont ete important comme composes structurels des membranes cellulaires et dans le developpement du systeme nerveux. Les activites des enzymes digestives ont ete controlees par leur genes et exprimes sequentiellement au cours du developpement. Elles hydrolysent le vitellus et fournissent les substances de construction et la source d'energie pour la formation des tissus, des organes et des differents systemes., The biochemical composition and the activity of five digestive enzymes of embryos were studied during embryonic development in the redclaw crayfish, Cherax quadricarinatus (Von Martens, 1868), in order to investigate the relationship between embryonic development and the utilization of yolk. Carbohydrates remained a minor constituent of the yolk as a whole. The contents of total proteins and total amino acids (TAA) were all observed to decrease. Among the essential amino acids (EAA), the contents of leucine and arginine were relatively high, while of the non-essential amino acids (NEAA), the most important components were glutamine and aspartic acid. The contents of total lipids (TL) decreased during embryonic development, and the predominant fatty acids of both neutral lipids (NL) and polar lipids (PL) were C16:0, C18:0, C18:1ω9, and C18:3ω3. The five digestive enzymes tested all showed changes in their activities. The specific activities of pepsin and trypsin gradually increased in the earlier stages of embryonic development, pepsin decreased rapidly in later stages, while trypsin still kept a high level of activity. The specific activity of amylase changed in a "V" shape and reached high values again in later stages. The specific activities of cellulase and lipase showed relatively low values. It is shown that carbohydrates may play an important role in synthesizing many specific compounds to participate in signal transmission and in forming the carapace. Proteins and lipids were the dominating construction and energy substances; proteins were primarily consumed in the early stages, as against lipids in later stages. Saturated fatty acids (SFA) (16:0 and 18:0) and monounsaturated fatty acids (MUFA) (16:1ω7 and 18:1ω9) were always used for energetic purposes and polyunsaturated fatty acids (PUFA) (20:5ω3 and 22:6ω3) were important as structural components of cell membranes and in the development of the central nervous system. The activities of the digestive enzymes were controlled by their genes and expressed sequentially during embryonic development. They hydrolyse the yolk and provide construction substances and energy sources for the formation of tissues, organs, and various systems in general. La composition biochimique et l'activite de cinq enzymes digestives d'embryons ont ete etudiees au cours du developpement embryonnaire de l'ecrevisse a patte rouge, Cherax quadricarinatus (Von Martens, 1868), dans le but d'investiguer les inter-relations entre le developpement embryonnaire et l'utilisation du vitellus. Les glucides representent un constituant mineur du vitellus. Les contenus en proteines totales et en acides amines totaux (TAA) diminuent. Parmi les AA essentiels (EAA), les contenus en leucine et arginine ont ete relativement eleves, tandis que parmi les acides amines non-essentiels (NEAA), les composes les plus importants ont ete la glutamine et l'acide aspartique. Les contenus en lipides totaux (TL) diminuent au cours du developpement embryonnaire, et les acides gras predominants parmi, a la fois les lipides neutres (NL) et les lipides polaires (PL), etaient C16:0, C18:0, C18:1ω9, et C18:3ω3. Les cinq enzymes digestives testees montrent toutes un changement dans leur activite. Les activites specifiques de la pepsine et de la trypsine augmentent progressivement au cours des premiers stades du developpement embryonnaire, la pepsine decroit rapidement dans les derniers stades, tandis que la trypsine conserve un degre d'activite eleve. L'activite specifique de l'amylase change selon une forme en V et atteint a nouveau un niveau eleve dans les derniers stades. Les activites specifiques de la cellulase et de la lipase montrent des valeurs relativement basses. Il est montre que les glucides pourraient jouer un role important en synthetisant de nombreux composes specifiques pour participer au signal de transmission et a la formation de la carapace. Les proteines et les lipides ont ete les substances dominantes pour la construction et l'energie; les proteines ont ete prioritairement utilisees dans les jeunes stades et les lipides dans les derniers stades. Les acides gras satures (SFA) (16:0 et 18:0) et les acides gras mono-unsatures (MUFA) (16:1ω7 et 18:1ω9) ont toujours ete utilises pour les besoins energetiques, et les acides gras poly-unsatures (PUFA) (20:5ω3 et 22:6ω3) ont ete important comme composes structurels des membranes cellulaires et dans le developpement du systeme nerveux. Les activites des enzymes digestives ont ete controlees par leur genes et exprimes sequentiellement au cours du developpement. Elles hydrolysent le vitellus et fournissent les substances de construction et la source d'energie pour la formation des tissus, des organes et des differents systemes.]
- Published
- 2008
39. MORPHOGENESIS AND VARIATIONS IN BIOCHEMICAL COMPOSITION OF THE EGGS OF MACROBRACHIUM NIPPONENSE (DE HAAN, 1849) (DECAPODA, CARIDEA, PALAEMONIDAE) DURING EMBRYONIC DEVELOPMENT
- Author
-
Yanmin Zhao, Yun-Long Zhao, and Cuo Zeng
- Subjects
food.ingredient ,Macrobrachium ,biology ,Embryogenesis ,Morphogenesis ,Embryo ,Anatomy ,Aquatic Science ,biology.organism_classification ,Blastula ,Cell biology ,food ,Yolk ,embryonic structures ,Animal Science and Zoology ,Macrobrachium nipponense ,Energy source - Abstract
Morphogenesis and variations in biochemical composition of the eggs of the prawn, Macrobrachium nipponense were studied during embryonic development. Eight morphological stages were defined. These are: the fertilized egg, cleavage, blastula, gastrula, egg-nauplius, egg-metanauplius, protozoea, and zoea. The whole embryogenesis lasts about 25 days at a water temperature of 18-22 ° C. Proteins, lipids, and carbohydrates were the main components of the embryos. During early embryonic development, protein content generally increased while the contents of lipid and carbohydrate decreased. It is suggested that the proteins in the yolk were used mainly in structural growth, whereas the lipids and carbohydrates were mainly used as energy sources. Lipid content increased while protein content decreased in the protozoea stage, showing that lipid is an important structural substance and protein can be utilized as an energy source, especially in late embryonic stages. Variations in biochemical composition were closely related to events in morphogenesis during embryonic development. The embryonic developmental stages used in the present study may be applied to other embryonic developmental studies of Macrobrachium , and be applicable in other palaemonids as well.
- Published
- 2007
40. The Cardioprotective Effects of Late-Phase Remote Preconditioning of Trauma Depends on Neurogenic Pathways and the Activation of PKC and NF-κB (But Not iNOS) in Mice
- Author
-
D. Y. Hou, Xiaoping Ren, Peng-Wei Li, Qing Miao, Yun-Long Zhao, Yang Song, and Yi-Jie Ye
- Subjects
0301 basic medicine ,Genetically modified mouse ,Male ,Time Factors ,Receptor, Bradykinin B2 ,Sensory Receptor Cells ,Myocardial Infarction ,Bradykinin ,Nitric Oxide Synthase Type II ,Apoptosis ,Myocardial Reperfusion Injury ,030204 cardiovascular system & hematology ,Pharmacology ,Synaptic Transmission ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,NF-KappaB Inhibitor alpha ,Abdomen ,Receptors, Adrenergic, beta ,Medicine ,Animals ,Pharmacology (medical) ,Genetic Predisposition to Disease ,Protein Kinase C ,Cardioprotection ,Mice, Knockout ,Thoracic Nerves ,biology ,business.industry ,Myocardium ,NF-kappa B ,NF-κB ,NFKB1 ,Nitric oxide synthase ,Enzyme Activation ,IκBα ,Disease Models, Animal ,030104 developmental biology ,Phenotype ,chemistry ,Anesthesia ,Knockout mouse ,biology.protein ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Background: A superficial abdominal surgical incision elicits cardioprotection against cardiac ischemia–reperfusion (I/R) injury in mice. This process, called remote preconditioning of trauma (RPCT), has both an early and a late phase. Previous investigations have demonstrated that early RPCT reduces cardiac infarct size by 80% to 85%. We evaluated the cardioprotective and molecular mechanisms of late-phase RPCT in a murine I/R injury model. Methods: Wild-type mice, bradykinin (BK) 2 receptor knockout mice, 3M transgenic mice (nuclear factor κB [NF-κb] repressor inhibitor of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha [IκBα(S32A, S36A, Y42F)]), and inducible nitric oxide synthase (iNOS) knockout mice were analyzed using a previously established I/R injury model. A noninvasive abdominal surgical incision was made 24 hours prior to I/R injury and the infarct size was determined at 24 hours post-I/R injury. Results: The results indicated that a strong cardioprotective effect occurred during late-phase RPCT (58.42% ± 1.89% sham vs 29.41% ± 4.00% late RPCT, mean area of the infarct divided by the mean area of the risk region; P ≤ .05; n = 10). Furthermore, pharmacological intervention revealed the involvement of neurogenic signaling in the beneficial effects of late RPCT via sensory and sympathetic thoracic nerves. Pharmacological experiments in transgenic mice-implicated BK receptors, β-adrenergic receptors, protein kinase C, and NF-κB but not iNOS signaling in the cardioprotective effects of late RPCT. Conclusion: Late RPCT significantly decreased myocardial infarct size via neurogenic transmission and various other signaling pathways. This protective mechanism differentiates late and early RPCT. This study describes a new cardiac I/R injury prevention method and refines the concept of RPCT.
- Published
- 2015
41. Effect of Waterborne Copper on the Microstructures of Gill and Hepatopancreas in Eriocheir sinensis and Its Induction of Metallothionein Synthesis
- Author
-
Jian Yang, Yun-long Zhao, Na Li, and Zhibiao Yang
- Subjects
Gills ,China ,Brachyura ,Health, Toxicology and Mutagenesis ,Hepatopancreas ,chemistry.chemical_element ,Toxicology ,Rivers ,medicine ,Animals ,Metallothionein ,Chinese mitten crab ,Dose-Response Relationship, Drug ,biology ,Decapoda ,Spectrophotometry, Atomic ,Copper toxicity ,General Medicine ,Anatomy ,medicine.disease ,biology.organism_classification ,Pollution ,Copper ,Molecular biology ,Dose–response relationship ,Eriocheir ,chemistry ,Water Pollutants, Chemical - Abstract
Effects of different waterborne copper concentrations (0.00, 0.01, 0.10, 1.00, and 5.00 mg/l) on microstructures of gill and hepatopancreas, and in inducing metallothionein (MT) synthesis were studied in Chinese mitten crab Eriocheir sinennsis. The results indicated that copper accumulation caused histologic damage to gill and hepatopancreas and induced MT synthesis in hepatopancreas. No significant differences were found in microstructures between 0.01 mg/l copper treatment and the control group. Exposure to copper concentrations ranging from 0.1 to 5.0 mg/l for 10 days resulted in irregularly thickened lamella, enlarged haemocoels, increased hemocytes, and damaged or disassembled epithelia in gill. Similarly, in hepatopancreas, the spaces of the small blind tubules decreased, and nuclei of the epithelia were karyopycnosised or karyorhexised. Also, the basement membranes were rumpled or even ruptured, with new vacuoles formed between the basement membrane and the simple columnar epithelia. MT measurements showed no significant differences in MT contents between the control group and the group treated with 0.01 mg/l waterborne copper. Maximum MT content was observed at the treatment level of 0.10 mg/l waterborne copper.
- Published
- 2006
42. Accumulation, Distribution, and Toxicology of Copper Sulfate in Juvenile Giant Freshwater Prawns, Macrobrachium rosenbergii
- Author
-
Yun-long Zhao, Na Li, and Jian Yang
- Subjects
Copper Sulfate ,biology ,Macrobrachium rosenbergii ,Ecology ,Decapoda ,Health, Toxicology and Mutagenesis ,Zoology ,Fresh Water ,Copper sulfate ,General Medicine ,Toxicology ,biology.organism_classification ,Pollution ,Crustacean ,Shrimp ,Animals ,Juvenile ,Ecotoxicology ,Palaemonidae - Published
- 2005
43. Effects of dietary protein and water exchange on water quality, survival and growth of postlarvae and juvenile Litopenaeus vannamei
- Author
-
Lan-mei Wang, Frank Castille, Addison L. Lawrence, and Yun-long Zhao
- Subjects
Litopenaeus ,Biology ,biology.organism_classification ,Shrimp ,chemistry.chemical_compound ,Ammonia ,Animal science ,Nitrate ,chemistry ,Biochemistry ,medicine ,Water quality ,Growth rate ,medicine.symptom ,Nitrite ,Weight gain - Abstract
Two growth trials were conducted with Litopenaeus vannamei to evaluate effects of dietary protein and water exchange on survival, growth and water quality. In both trials, protein levels were 12, 15, 20, 26 and 35%. In the first trial, 6.21 g juvenile shrimp were stocked for 23 days at either zero or high (2750% daily) water exchange. At high exchange, survival was greater than 93% for all protein levels. Final body weight (FBW) and weight gain (WG) increased with protein level from 12% to 20% (P < 0:05). FBW and WG at 20 and 26% protein were lower than that at 35% protein. At zero exchange, survival decreased with protein above 20%. At zero exchange, water quality decreased (high ammonia, nitrite, nitrate and low pH, alkalinity) with protein greater than 15%. WG with 12% protein was greater at zero exchange than at high exchange. In the second trial, 0.22 g postlarvae were stocked for 26 days at either zero or high (5440% daily) water exchange. At high exchange, survival was 90% or greater for all protein levels. FBW and WG increased with protein level from 12% to 20% (P < 0:05). At zero exchange, FBW and WG were maximum with 20% protein. Survival was lowest at 35% protein. For 35% protein, survival was lower at zero than at high exchange. For all protein levels except 35%, WG was higher at zero than at high exchange. The results suggest that lower protein diets can replace high protein (35%) commercial diets and obtain high growth rate for both juvenile and postlarvae L. vannamei at zero exchange. Further, a 20% protein diet, which contained 25.3% marine animal meals, was adequate for shrimp growth, survival and water quality at zero exchange.
- Published
- 2017
44. Effects of water exchange and reducing dietary vitamin and mineral supplementation on survival and growth of Litopenaeus vannamei
- Author
-
Yun-long Zhao, Lan-mei Wang, Frank Castille, and Addison L. Lawrence
- Subjects
Vitamin ,Fishery ,chemistry.chemical_compound ,Animal science ,chemistry ,Litopenaeus ,Water quality ,Water exchange ,Mineral supplementation ,Biology ,biology.organism_classification ,Dietary vitamin - Abstract
A growth trial was conducted with Litopenaeus vannamei to evaluate effects of dietary vitamin and mineral supplementation (VMS) and water exchange on survival, growth and water quality. Four levels (0, 25, 50 and 100%) of VMS were evaluated using a 20% protein base diet. Postlarvae weighing 0.22 g were stocked for 26 days with either zero or high (5440% daily) water exchange. Growth was greater at zero than high exchange. However, growth was not affected by the level of VMS at both high and zero exchange. Survival for 0% VMS was lower than survivals for 25 to 100% VMS at high exchange. For 0% VMS, survival at high exchange was lower than survival at zero exchange. Results suggested that at zero water exchange, diets without VMS can replace diets with VMS without reducing survival.
- Published
- 2017
45. Face recognition method based on relative gradient
- Author
-
Kun Deng, Yun-long Zhao, Hong-zhi Gao, and Lu Yao
- Subjects
business.industry ,Computer science ,Three-dimensional face recognition ,Pattern recognition ,Artificial intelligence ,business ,Facial recognition system - Published
- 2009
46. Crystal structure of 1,8-bis[4-(4-pentylcyclohexyl)phenyl]-10- methoxyanthracene, C49H60O
- Author
-
Yun-Long Zhao and Juan Qiao
- Subjects
Inorganic Chemistry ,Crystallography ,QD901-999 ,Chemistry ,General Materials Science ,Crystal structure ,Condensed Matter Physics - Published
- 2009
47. [Variations of zooplankton's community structure in reclaimed waters of Nanhui east tidal flat of Shanghai, East China]
- Author
-
Qiang, Li, Chang-An, Ma, Wei-Wei, Lü, Wei, Tian, Ji, Yu, and Yun-Long, Zhao
- Subjects
China ,Oceans and Seas ,Wetlands ,Population Dynamics ,Animals ,Biodiversity ,Ecosystem ,Zooplankton ,Environmental Monitoring - Abstract
From October 2010 to July 2011, an investigation was conducted on the zooplankton communities in reclaimed and natural waters in Nanhui east tidal flat to study the seasonal variations of the zooplankton's species composition, abundance, biomass, dominant species, and biodiversity, aimed to explore the differences in the zooplankton's community structure within and outside the reclaimed waters and the relationships of the community structure with several indispensable environmental factors such as water salinity, water temperature, and human activities. A total of 30 zooplankton species were identified, among which, 24 species were in reclaimed waters and dominated by rotifers, and 14 species were in natural waters and dominated by copepods. The average abundance of the zooplankton was obviously higher in reclaimed waters than in natural waters, but the average biomass was in adverse. The dominant species in reclaimed waters were freshwater species, such as Brachionus angularis, B. calyciflorus, and Mesocyclops leuckarti, while those in natural waters were brackish species, such as Sinocalanus sinensis, Schmackeria poplesia, and Tortanus vermiculus. Both in reclaimed and in natural waters, the dominant species had seasonal alternation. The Shannon index (H) and Pielou evenness index (J) of the zooplankton communities were higher in natural waters, but the Margalef species richness (d) and simplicity index (C) were higher in reclaimed waters. Cluster analysis and MDS evaluation indicated that there was an obvious difference in the community structure of zooplankton between reclaimed and natural waters. Reclamation could be the primary cause for the variations of the zooplankton's community structure in reclaimed waters, and environmental factors such as water salinity and tidal power also contributed to the variations.
- Published
- 2012
48. Feature selection via minimizing nearest neighbor classification error
- Author
-
Tian-Hang Meng, Yun-Long Zhao, Qinghua Hu, Pengfei Zhu, and Rui-Xian Ma
- Subjects
Artificial neural network ,Computer science ,business.industry ,Feature extraction ,Linear classifier ,Feature selection ,Pattern recognition ,computer.software_genre ,k-nearest neighbors algorithm ,Statistical classification ,Feature (machine learning) ,Data mining ,Artificial intelligence ,Gradient descent ,business ,computer - Abstract
Feature selection is viewed as an important preprocessing step for pattern recognition, machine learning and data mining. It is used to find an optimal subset to reduce computational cost, increase the classification accuracy and improve result comprehensibility. In this paper, a weighted distance learning approach is introduced to minimize Leaving-One-Out classification error using a gradient descent algorithm. The quality of features is evaluated with the learned weight and the features with great weights are considered to be useful for classification. Experimental analysis shows that the proposed approach has better performance than several state-of-the-art methods.
- Published
- 2010
49. Effects of Water-Borne Copper on Isozymes of Gill in Eriocheir sinensis
- Author
-
Yun-long Zhao, Yu-jie Fan, Jianguo Zhu, Xiuguo Hua, Wen Zhang, Jian Yang, Zhibiao Yang, Li Cui, Congli Yuan, and Na Li
- Subjects
inorganic chemicals ,Gill ,chemistry.chemical_classification ,biology ,chemistry.chemical_element ,biology.organism_classification ,Isozyme ,Molecular biology ,Copper ,Electrophoresis ,Eriocheir ,Enzyme ,chemistry ,Polyacrylamide gel electrophoresis - Abstract
By using the PAGE (Poly Acrylamide Gel Electrophoresis), the effect of water-borne Cu2+ on the isozymes in gills of E. sinensis was studied. The experimental groups were treated with different concentration of Cu2+ as 0.01, 0.10, 1.00, and 5.00 mg/l, respectively. The results showed that different isozymes had specific responses. The activity of LDH and MDH decreased gradually along with the increasing of Cu2+ level. Increasing of Cu2+ level followed by decreasing of ALP was observed, and the variation of EST wasn't significant. These isozymes variations reflected the toxic effects of Cu2+ sensitively.
- Published
- 2010
50. Stenus indubius
- Author
-
Tang, Liang and Yun-Long, Zhao
- Subjects
Coleoptera ,Insecta ,Arthropoda ,Animalia ,Biodiversity ,Stenus ,Staphylinidae ,Stenus indubius ,Taxonomy - Abstract
Key to Chinese species of the indubius group 1. Pronotum with median longitudinal furrow; elytra with surface very uneven; 4 th to 6 th abdominal segments with tergites and sternites distinctly separated (Hemistenus)............................................................ 2 - Pronotum without median longitudinal furrow; elytra with surface weakly uneven; 4 th to 6 th abdominal segments with tergites and sternites completely fused without joint suture (Hypostenus)....................... 4 2. Abdominal punctation denser, interstices on 3 rd to 7 th abdominal tergites distinctly smaller than half diameter of punctures; posterior margin of 7 th abdominal sternite of male without emargination. Aedeagus as in Fig. 11, spermatheca as in Fig. 13. BL: 4.0��� 4.2 mm. China: Sichuan.......................................... ................................................................................................................... S. pectorifossatus Tang, sp. nov. - Abdominal punctation sparser, interstices on 3 rd to 7 th abdominal tergites smaller than half diameter of punctures; posterior margin of 7 th abdominal sternite of male with slight median emargination.............. 3 3. Body larger (BL: 3.8���4.2 mm); median longitudinal furrow and impressions on pronotum very deep. Aedeagus as in Fig. 16, spermatheca as in Fig. 18. China: Sichuan......... S. erlangshanus Tang, sp. nov. - Body smaller (BL: 3.0��� 3.7 mm); median longitudinal furrow and impressions on pronotum relatively shallow. Aedeagus as in Fig. 6, spermatheca as in Fig. 8.. China: Sichuan... S. zhuxiaoyui Tang, sp. nov. 4. Body larger (BL: 4.3���4.8 mm), elytra shorter (EL: EW= 43.5: 47.0). Aedeagus as in Fig. 9 (in Tang & Li 2005), spermatheca as in Fig. 10 (in Tang & Li 2005). China: Anhui......... S. guniujiangensis Tang & Li - Body smaller (BL: 3.3���3.5 mm), elytra longer (EL: EW=38.0: 37.5). Aedeagus as in Fig. 5 (in Tang & Li 2005), spermatheca as in Fig. 6 (in Tang & Li 2005). China: Anhui ................... S. paradecens Tang & Li, Published as part of Tang, Liang & Yun-Long, Zhao, 2008, Three new Stenus species of the indubius group (Coleoptera, Staphylinidae) from China, pp. 51-58 in Zootaxa 1741 on page 57, DOI: 10.5281/zenodo.181503, {"references":["Tang, L. & Li, L. - Z. (2005) Two new species of Stenus from Anhui Province, East China (Coleoptera: Staphylinidae). [In Chinese] In: Ren, G. D. (Ed.) Classification and Diversity of Insects in China. China Agriculture Science and Technology Press, Beijing, pp. 106 - 109."]}
- Published
- 2008
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