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3. Facile synthesis of hydroxypropyl chitosan-egg white hydrogel dressing with antibacterial and antioxidative activities for accelerating the healing of burn wounds

Author

Ying Zhao, Yulan Zhao, Yuguo Chu, and Qiang Chang

Subjects
Biomedical Engineering, General Materials Science, General Chemistry, General Medicine
Abstract

A facile self-healing hydrogel composed of egg white and hydroxypropyl chitosan with excellent biocompatibility as well as antioxidant, anti-inflammation, and antibacterial activities was prepared to accelerate the healing of burn wounds.

Published
2023

4. Protein kinase A participates in hyphal and appressorial development by targeting Efg1‐mediated transcription of a Rab <scp>GTPase</scp> in Setosphaeria turcica

Author

Yuwei Liu, Shen Shen, Zhimin Hao, Qing Wang, Yumei Zhang, Yulan Zhao, Yameng Tong, Fanli Zeng, and Jingao Dong

Subjects
Hyphae, Soil Science, Chitin, Plant Science, Cyclic AMP-Dependent Protein Kinases, Adenosine Monophosphate, Fungal Proteins, Ascomycota, rab GTP-Binding Proteins, Gene Expression Regulation, Fungal, Cyclic AMP, Agronomy and Crop Science, Molecular Biology, Transcription Factors
Abstract

The cyclic adenosine monophosphate (cAMP) signalling pathway plays an important role in the regulation of the development and pathogenicity of filamentous fungi. cAMP-dependent protein kinase A (PKA) is the conserved element downstream of cAMP, and its diverse mechanisms in multiple filamentous fungi are not well known yet. In the present study, gene knockout mutants of two catalytic subunits of PKA (PKA-C) in Setosphaeria turcica were created to illustrate the regulatory mechanisms of PKA-Cs on the development and pathogenicity of S. turcica. As a result, StPkaC2 was proved to be the main contributor of PKA activity in S. turcica. In addition, it was found that both StPkaC1 and StPkaC2 were necessary for conidiation and invasive growth, while only StPkaC2 played a negative role in the regulation of filamentous growth. We reveal that only StPkaC2 could interact with the transcription factor StEfg1, and it inhibited the transcription of StRAB1, a Rab GTPase homologue coding gene in S. turcica, whereas StPkaC1 could specifically interact with a transcriptional regulator StFlo8, which could rescue the transcriptional inhibition of StEfg1 on StRAB1. We also demonstrated that StRAB1 could positively influence the biosynthesis of chitin in hyphae, thus changing the filamentous growth. Our findings clarify that StPkaC2 participates in chitin biosynthesis to modulate mycelium development by targeting the Efg1-mediated transcription of StRAB1, while StFlo8, interacting with StPkaC1, acts as a negative regulator during this process.

Published
2022

6. DeepRCI: Predicting ATP-Binding Proteins Using the Residue-Residue Contact Information

Author

Zhaoxi Zhang, Yulan Zhao, Juan Wang, and Maozu Guo

Subjects
Adenosine Triphosphate, Health Information Management, Polyphosphates, Adenine, Computational Biology, Humans, Proteins, Health Informatics, Electrical and Electronic Engineering, Carrier Proteins, Algorithms, Computer Science Applications
Abstract

Adenine-5'-triphosphate (ATP) is a direct energy source for various activities of tissues and cells in the body. The release of ATP energies requires the assistance of ATP-binding proteins. Therefore, the identification of ATP-binding proteins is of great significance for the research on organisms. So far, there are several methods for predicting ATP-binding proteins. However, the accuracies of these methods are so low that the predicted proteins are inaccurate. Here, we designed a novel method, called as DeepRCI (based on Deep convolutional neural network and Residue-residue Contact Information), for predicting ATP-binding proteins. In order to maximize the performance of our method, we experimented with different hyperparameters and finally chose a 12-depth-512-filters deep convolutional neural network with an input size of 448*448. By using this model, DeepRCI achieved an accuracy of 93.61% on the test set which means a significant improvement of 11.78% over the state-of-the-art methods. We also compared the performance of residue-residue contact information datasets with different noise levels which are mainly due to gaps in the multiple sequence alignment. Compared with the low-noise dataset, the prediction accuracy on the high-noise dataset is reduced by 6.78%, which affects the performance of DeepRCI to a certain extent. We believe that with the increase of sequence data, this problem will eventually be solved. Finally, we provide a web service of DeepRCI which link can be obtained in Data Availability.

Published
2022

8. Tooth loss and the risk of cognitive decline and dementia: A meta-analysis of cohort studies

Author

Liqing Li, Qi Zhang, Di Yang, Sule Yang, Yulan Zhao, Min Jiang, Xiaofang Wang, Ling Zhao, Qi Liu, Zuxun Lu, Xiaogang Zhou, Yong Gan, and Chunmei Wu

Subjects
Neurology, Neurology (clinical)
Abstract

IntroductionEpidemiological studies have shown that tooth loss may be associated with an increased risk of cognitive decline and dementia. However, some results do not show a significant association. Therefore, we performed a meta-analysis to evaluate this association.MethodsRelevant cohort studies were searched in PubMed, Embase, Web of Science (up to May 2022), and the reference lists of retrieved articles. The pooled relative risk (RR) and 95% confidence intervals were computed using a random-effects model (CI). Heterogeneity was evaluated using the I2 statistic. Publication bias was evaluated using the Begg's and Egger's tests.ResultsEighteen cohort studies met the inclusion criteria. Original studies with 356,297 participants with an average follow-up of 8.6 years (ranging from 2 to 20 years) were included in this study. The pooled RRs of tooth loss on dementia and cognitive decline were 1.15 (95% CI: 1.10–1.20; P < 0.01, I2 = 67.4%) and 1.20 (95% CI: 1.14–1.26; P = 0.04, I2 = 42.3%), respectively. The results of the subgroup analysis showed an increased association between tooth loss and Alzheimer's disease (AD) (RR = 1.12, 95% CI: 1.02–1.23) and vascular dementia (VaD) (RR = 1.25, 95% CI: 1.06–1.47). The results of the subgroup analysis also showed that pooled RRs varied by geographic location, sex, use of dentures, number of teeth or edentulous status, dental assessment, and follow-up duration. None of the Begg's and Egger's tests or funnel plots showed evidence of publication bias.DiscussionTooth loss is associated with a significantly increased risk of cognitive decline and dementia, suggesting that adequate natural teeth are important for cognitive function in older adults. The likely mechanisms mostly suggested include nutrition, inflammation, and neural feedback, especially deficiency of several nutrients like vitamin D.

Published
2023

10. Supplementary Figures S1-S8 from USP22 Deubiquitinates CD274 to Suppress Anticancer Immunity

Author

Xueli Bai, Tingbo Liang, Qi Chen, Yulan Zhao, Shanshan Li, Xiaozhen Zhang, Lin Wang, Xinyu Zhao, Junli Wang, Yu Lou, Qi Zhang, and Xing Huang

Abstract

This file contains supplemental figures S1-S8, and the detailed description is as follows: Figure S1 shows USP22 binding to HLEET motif of CD274. Figure S2 shows USP22 stabilizing CD274 in CDK4-independent manner. Figure S3 shows the non-requirement of CD274 for USP22-controlled cancer cell growth in vitro. Figure S4 shows the expression profile of USP22 in multiple cancers. Figure S5 shows the CD274-independent roles of USP22 in liver cancer growth and therapy. Figure S6 shows the expression levels of CD274 in USP22-regulated tumor immunity. Figure S7 shows the negative correlation of USP22 and immune signatures in LIHC. Figure S8 shows the schematic model.

Published
2023

12. Data from Analysis of MiR-195 and MiR-497 Expression, Regulation and Role in Breast Cancer

Author

Lihui Lai, Bing-Hua Jiang, Feng Mao, Ling-Zhi Liu, Hallgeir Rui, Hsiang-Fu Kung, Marie C. Lin, Andreas Dress, Zhenbing Zeng, Qiang Sun, Dan Zhao, Xuejing Wang, Shunying Liu, Xiaolong Zhang, Chao Zou, Yue Jiang, Yanting Qi, Xiaona Chen, Changxing Liu, Yulan Zhao, and Dan Li

Abstract

Purpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer.Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues.Results: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer.Conclusions: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets. Clin Cancer Res; 17(7); 1722–30. ©2011 AACR.

Published
2023

15. Application Experience of Moist Wound Healing Theory Combined with Modern New Dressings in the Treatment of Pressure Ulcers

Author

Yulan Zhao, Mingjuan Zhao, and Na Zhao

Subjects
medicine.medical_specialty, business.industry, Granulation tissue, % diameter reduction, Growth time, Surgery, medicine.anatomical_structure, Edema, Medicine, In patient, Treatment effect, medicine.symptom, business, Wound healing, Clinical treatment
Abstract

Objective?Research on the effect of moist wound healing theory in a combination with modern new dressingtreatment in patients diagnosed with pressure ulcers. Method: Selected 30 patients with pressure ulcers from our hospital, which is Shandong Tai’an Municipal Hospital, from January 2019 to January 2021 were divided into experimental group (15 cases, treated with moist wound healing theory combined with modern new dressings) and control group (15 cases, applied conventional treatments). The treatment effect, time of wound edema subsidence, wound healing time, number of dressing changes, granulation tissue growth time, and diameter reduction time were compared between the two groups. Results: The total effective rate of the experimental group (93.33%, 14/15) was higher than that of the control group (53.33%, 8/15), P

Published
2021

16. Initial Invasive or Conservative Strategy for Stable Coronary Disease

Author

Maron D. J., Hochman J. S., Reynolds H. R., Bangalore S., O'Brien S. M., Boden W. E., Chaitman B. R., Senior R., Lopez-Sendon J., Alexander K. P., Lopes R. D., Shaw L. J., Berger J. S., Newman J. D., Sidhu M. S., Goodman S. G., Ruzyllo W., Gosselin G., Maggioni A. P., White H. D., Bhargava B., Min J. K., John Mancini G. B., Berman D. S., Picard M. H., Kwong R. Y., Ali Z. A., Mark D. B., Spertus J. A., Krishnan M. N., Elghamaz A., Moorthy N., Hueb W. A., Demkow M., Mavromatis K., Bockeria O., Peteiro J., Miller T. D., Szwed H., Doerr R., Keltai M., Selvanayagam J. B., Gabriel Steg P., Held C., Kohsaka S., Mavromichalis S., Kirby R., Jeffries N. O., Harrell F. E., Rockhold F. W., Broderick S., Bruce Ferguson T., Williams D. O., Harrington R. A., Stone G. W., Rosenberg Y, ISCHEMIA Research Group: Joseph Ricci, A Tello Montoliu, A I Robero Aniorte, Abbey Mulder, Abhay A Laddu, Abhinav Goyal, Abhishek Dubey, Abhishek Goyal, Abigail Knighton, Abraham Oomman, Adam J Jaskowiak, Adam Kolodziej, Adam Witkowski, Adnan Hameed, Adriana Anesini, Afshan Hussain, Agne Juceviciene, Agne Urboniene, Agnes Jakal, Agnieszka Szramowska, Ahmad Khairuddin, Ahmed Abdel-Latif, Ahmed Adel, Ahmed Aljzeeri, Ahmed Kamal, Ahmed Talaat, Aimee Mann, Aira Contreras, Ajit Kumar, V K Kumar, Akemi Furukawa, Akshay Bagai, Akvile Smigelskaite, Alain Furber, Alain Rheault, Alaine Melanie Loehr, Alan Rosen, Albert Varga, Albertina Qelaj, Alberto Barioli, Aldo Russo, Alec Moorman, Alejandro Gisbert, Aleksandra Fratczak, Aleksandras Laucevicius, Alena Kuleshova, Alessandro Sionis, Alexander A Sirker, Alexander M Chernyavskiy, Alexandra Craft, Alexandra Vazquez, Alexandre Ciappina Hueb, Alexandre S Colafranseschi, Alexandre Schaan de Quadros, Alexandre Tognon, Ali Alghamdi, Alice Manica Muller, Aline Nogueira Rabaça, Aline Peixoto Deiro, Alison Hallam, Allegra Stone, Allison Schley, Almudena Castro, Alvaro Rabelo Ales, Amanda Germann, Amanda O'Malley, Amar Uxa, Amarachi Ojajuni, Amarino C Oliveira Jr, Amber B Hull, Ambuj Roy, Amer Zarka, Amir Janmohamed, Ammani Brown, Ammy Malinay, Amparo Martinez Monzonis, Amy J Richards, Amy Iskandrian, Amy Ollinger, Ana D Djordjevic-Dikic, Ana Fernández Martínez, Ana Gomes Almeida, Ana Paula Batista, Ana Rita Francisco, Ana S Mladenovic, Ana Santana, Anam Siddiqui, Anastasia M Kuzmina-Krutetskaya, Andras Vertes, Andre S Sousa, Andre Gabriel, André Schmidt, Andrea M Lundeen, Andrea Bartykowszki, Andrea Lorimer, Andrea Mortara, Andrea Pascual, Andreia Coelho, Andreia Rocha, Andrés García-Rincón, Andrew G Howarth, Andrew J Moriarty, Andrew Docherty, Andrew Starovoytov, Andrew Zurick, Andrzej Łabyk, Andrzej Swiatkowski, Andy Lam, Anelise Kawakami, Angela Hoye, Angela Kim, Angelique Smit, Angelo Nobre, Anil V Shah, Anja Ljubez, Anjali Anand, Ankush Sachdeva, Ann Greenberg, Ann Luyten, Ann Ostrander, Anna Di Donato, Anna Cichocka-Radwan, Anna Fojt, Anna Plachcinska, Anna Proietti, Anna Teresinska, Anne Marie Webb, Anne Cartwright, Anne Heath, Anne Mackin, Anong Amaritakomol, Anong Chaiyasri, Anoop Chauhan, Anoop Mathew, Anthony Gemignani, Anto Luigi Andres, Antonia Vega, Antonietta Hansen, Antonino Ginel Iglesias, Antonio Carlos Carvalho, Antonio Di Chiara, Antonio Serra Peñaranda, Antonio Carvalho, Antonio Colombo, Antonio Fiarresga, Anupama Rao, Aquiles Valdespino-Estrada, Araceli Boan, Areef Ishani, Ariel Diaz, Arijit Ghosh, Arintaya Prommintikul, Arline Roberts, Arnold H Seto, Arnold P Good, Arshed Quyyumi, Arthur J Labovitz, Arthur Kerner, Arturo S Campos-Santaolalla, Arunima Misra, Ashok Mukherjee, Ashok Seth, Ashraf Seedhom, Asim N Cheema, Asker Ahmed, Atul Mathur, Atul Verma, Audrey W Leong, Axel Åkerblom, Axelle Fuentes, Aynun Naher, Badhma Valaiyapathi, Baljeet Kaur, Bandula Guruge, Barbara Brzezińska, Barbara Nardi, Bartosz Czarniak, Bebek Singh, Begoña Igual, Bela Merkely, Belen Cid Alvarez, Benjamin J Spooner, Benjamin J W Chow, Benjamin Cheong, Benoy N Shah, Bernard de Bruyne, Bernardas Valecka, Bernhard Jäger, Beth A Archer, Beth Abramson, Beth Jorgenson, Bethany Harvey, Betsy O'Neal, Bev Atkinson, Bev Bozek, Bevin Lang, Bijulal Sasidharan, Bin Yang, Bin Zhang, Binoy Mannekkattukudy Kurian, Bjoern Goebel, Bob Hu, Bogdan A Popescu, Bogdan Crnokrak, Bolin Zhu, Bonnie J Kirby, Brandi D Zimbelman, Brandy Starks, Branko D Beleslin, Brenda Hart, Brian P Shapiro, Brian McCandless, Brianna Wisniewski, Brigham R Smith, Brooks Mirrer, Bruce McManus, Bruce Rutkin, Bruna Edilena Paulino, Bruna Maria Ascoli, Bryn Smith, Byron J Allen, C Michael Gibson, C Noel Bairey Merz, Calin Pop, Cameron Hague, Camila Thais de Ormundo, Candace Gopaul, Candice P Edillo, Carísi A Polanczyk, Carita Krannila, Carla Vicente, Carl-Éric Gagné, Carlo Briguori, Carlos Peña Gil, Carlos Alvarez, Carly Ohmart, Carmen C Beladan, Carmen Ginghina, Carol M Kartje, Caroline Alsweiler, Caroline Brown, Caroline Callison, Caroline Pinheiro, Caroline Rodgers, Caroline Spindler, Carolyn Corbett, Carrie Drum, Casey Riedberger, Catherine Bone, Catherine Fleming, Catherine Gordon, Catherine Jahrsdorfer, Catherine Lemay, Catherine Weick, Cathrine Patten, Cecilia Goletto, Cezary Kepka, Chandini Suvarna, Chang Xu, Chantale Mercure, Charle A Viljoen, Charlene Wiyarand, Charles Jia-Yin Hou, Charles Y Lui, Charles Cannan, Charles Cornet, Charlotte Pirro, Chataroon Rimsukcharoenchai, Chen Wang, Cheng-Ting Tsai, Chen-Yen Chien, Cheryl A Allardyce, Chester M Hedgepeth, Chetan Patel, Chiara Attanasio, Chih-Hsuan Yen, Chi-Ming Chow, Ching Min Er, Ching-Ching Ong, Cholenahally Nanjappa Manjunath, Chris Beck, Chris Buller, Christel Vassaliere, Christian Hamm, Christiano Caldeira, Christie Ballantyne, Christina Björklund, Christine R Hinton, Christine Bergeron, Christine Masson, Christine Roraff, Christine Shelley, Christophe Laure, Christophe Thuaire, Christopher Kinsey, Christopher McFarren, Christopher Spizzieri, Christopher Travill, Chun-Chieh Liu, Chung-Lieh Hung, Chunguang Li, Chun-Ho Yun, Chunli Xia, Ciarra Heard, Cidney Schultz, Clare Venn-Edmonds, Claudia P Hochberg, Claudia Wegmayr, Claudia Cortés, Claudia Escobar, Cláudia Freixo, Claudio T Mesquita, Clemens T Kadalie, Colin Berry, Constance Philander, Corine Thobois, Costantino Costantini, Courtney Page, Craig Atkinson, Craig Barr, Craig Paterson, Cristina Bare, Cynthia Baumann, Cynthia Burman, Dalisa Espinosa, Damien Collison, Dan Deleanu, Dan Elian, Dan Gao, Dana Oliver, Daniel P Vezina, Daniel O'Rourke, Daniele Komar, Danielle Schade, Darrel P Francis, Dastan Malaev, David A Bull, David E Winchester, David P Faxon, David Booth, David Cohen, David DeMets, David Foo, David Schlichting, David Taggart, David Waters, David Wohns, Davis Vo, Dawid Teodorczyk, Dawn Shelstad, Dawn Turnbull, Dayuan Li, Dean Kereiakes, Deborah O'Neill, Deborah Yip, Debra K Johnson, Debra Dees, Deepak L Bhatt, Deepika Gopal, Deepti Kumar, Deirdre Mattina, Deirdre Murphy, Delano R Small, Delsa K Rose, Dengke Jiang, Denis Carl Phaneuf, Denise Braganza, Denise Fine, Derek Cyr, Desiree Tobin, Diana Cukali, Diana Parra, Diane Camara, Diane Minshall Liu, Diego Adrián Vences, Diego Franca de Cunha, Dimitrios Stournaras, Dipti Patel, Dongze Li, Donna Exley, Dorit Grahl, Dragana Stanojevic, Duarte Cacela, Dwayne S G Conway, E Pinar Bermudez, Eapen Punnoose, Edgar L Tay, Edgar Karanjah, Edoardo Verna, Eduardo Hernandez-Rangel, Edward D Nicol, Edward O McFalls, Edward T Martin, Edyta Kaczmarska, Ekaterina I Lubinskaya, Elena A Demchenko, Elena Refoyo Salicio, Eli Feen, Elihú Durán-Cortés, Elisabeth M Janzen, Elise L Hannemann, Elise van Dongen, Elissa Restelli Piloto, Eliza Kaplan, Elizabeta Srbinovska Kostovska, Elizabeth Capasso-Gulve, Elizabeth Congdon, Elizabeth Ferguson, Elizaveta V Zbyshevskaya, Ellen Magedanz, Ellie Fridell, Ellis W Lader, Elvin Kedhi, Emanuela Racca, Emilie Tachot, Emily DeRosa, Encarnación Alonso-Álvarez, Eric Nicollet, Eric Peterson, Erick Alexánderson Rosas, Erick Donato Morales, Erin Orvis, Ermina Moga, Estelle Montpetit, Estevao Figueiredo, Eugene Passamani, Eugenia Nikolsky, Eunice Yeoh, Evgeniy I Kretov, Ewa Szczerba, Ewelina Wojtala, Expedito Eustáquio Ribeiro Silva, F Marin Ortuño, Fabio R Farias, Fabio Fimiani, Fabrizio Rolfo, Fa-Chang Yu, Fadi Hage, Fadi Matar, Fahim Haider Jafary, Fang Feng, Fang Liu, Fatima Ranjbaran, Fatima Rodriguez, Fausto J Pinto, Fauzia Rashid, Federica Ramani, Fei Wang, Fernanda Igansi, Filipa Silva, Filippo Ottani, Fiona Haines, Firas Al Solaiman, Flávia Egydio, Flavio Lyra, Florian Egger, Fran Farquharson, Frances Laube, Francesc Carreras Costa, Francesca de Micco, Francesca Bianchini, Francesca Pezzetta, Francesca Pietrucci, Francesco Orso, Francesco Pisano, Francis Burt, Francisca Patuleia Figueiras, Francisco Fernandez-Aviles, Francois Pierre Mongeon, Frans Van de Werf, Franziska Guenther, Fraser N Witherow, Fred Mohr, Frederico Dall'Orto, Fumiyuki Otsuka, G De La Morena, G Karthikeyan, Gabor Dekany, Gabor Kerecsen, Gabriel Galeote, Gabriel Grossmann, Gabriel Vorobiof, Gabriela Sanchez de Souza, Gabriela Guzman, Gabriela Zeballos, Gabriele Gabrielli, Gabriele Jakl-Kotauschek, Gail A Shammas, Gail Brandt, Gang Chen, Gary E Lane, Gary J Luckasen, Gautam Sharma, Gelmina Mikolaitiene, Gennie Yee, Georg Nickenig, George E Revtyak, George J Juang, Gerald Fletcher, Gerald Leonard, Gerard Patrick Devlin, Gerard Esposito, Gergely Ágoston, Gervasio Lamas, Geza Fontos, Ghada Mikhail, Gia Cobb, Gian Piero Perna, Gianpiero Leone, Giles Roditi, Gilles Barone-Rochette, Girish Mishra, Giuseppe Tarantini, Glenda Wong, Glenn S Hamroff, Glenn Rayos, Gong Cheng, Gonzalo Barge-Caballero, Goran Davidović, Goran Stankovic, Gordana Stevanovic, Grace Jingyan Wang, Grace M Young, Graceanne Wayser, Graciela Scaro, Graham S Hillis, Graham Wong, Grazyna Anna Szulczyk, Gregor Simonis, Gregory Kumkumian, Gretchen Ann Peichel, Grzegorz Gajos, Gudrun Steinmaurer, Guilherme G Rucatti, Guilherme Portugal, Guilhermina Cantinho Lopes, Guillem Pons Lladó, Gunnar Frostfelt, Gurpreet S Wander, Gurpreet Gulati, Gustavo Pucci, Hafidz Abd Hadi, Haibo Zhang, Haitao Wang, Halina Marciniak, Han Chen, Hanan Kerr, Hani Najm, Hanna Douglas, Hannah Phillips, Hao Dai, Haojian Dong, Haqeel Jamil, Harikrishnan Sivadasanpillai, Harry Suryapranata, Hassan Reda, Hayley Pomeroy, Heather Barrentine, Heather Golden, Heather Hurlburt, Heidi Wilson, Helen C Tucker, Helene Abergel, Hemalata Siddaram, Hermine Osseni, Herwig Schuchlenz, Hesong Zeng, Hicham Skali, Hilda Solomon, Hollie Horton, Holly Hetrick, Holly Little, Holly Park, Hongjie Chi, Hossam Mahrous, Howard A Levite, Hristo Pejkov, Huajun Li, Hugo Bloise-Adames, Hugo Marques, Hui Zhong, Hui-Min Zhang, Humayrah Hashim, Hung-I Yeh, Hussien El Fishawy, Ian Webb, Iftikhar Kullo, Igor O Grazhdankin, Ihab Hamzeh, Ikraam Hassan, Ikuko Ueda, Ileana L Pina, Ilona Tamasauskiene, Ilse Bouwhuis, Imran Arif, Ina Wenzelburger, Inês Zimbarra Cabrita, Ines Rodrigues, Inga H Robbins, Inga Soveri, Ingela Schnittger, Iqbal Karimullah, Ira M Dauber, Iram Rehman, Irena Peovska Mitevska, Irene Marthe Lang, Irina Subbotina, Irma Kalibataite-Rutkauskiene, Irni Yusnida, Isabel Estela Carvajal, Isabella C Palazzo, Isabelle Hogan, Isabelle Roy, Ishba Syed, Ishita Tejani, Ivan A Naryshkin, Ivana Jankovic, Iwona Niedzwiecka, J David Knight, Jacek Kusmierek, Jackie M White, Jackie Chow, Jacob Udell, Jacqueline E Tamis-Holland, Jacqueline Fannon, Jacquelyn A Quin, Jacquelyn Do, Jaekyeong Heo, Jakub Maksym, James E Davies, James H O'Keefe Jr, James J Jang, James Cha, James Harrison, James Hirsch, James Stafford, James Tatoulis, Jamie Rankin, Jan Henzel, Jan Orga, Jana Tancredi, Janaina Oliveira, Jane Burton, Jane Eckstein, Jane Marucci, Janet P Knight, Janet Blount, Janet Halliday, Janetta Kourzenkova, Janitha Raj, Jan-Malte Sinning, Jaqueline Pozzibon, Jaroslaw Drozdz, Jaroslaw Karwowski, Jason D Glover, Jason Loh Kwok, Jason T Call, Jason Linefsky, Jassira Gomes, Jati Anumpa, Javier J Garcia, Javier Courtis, Jay Meisner, K Jayakumar, Jayne Scales, Jean E Denaro, Jean Michel Juliard, Jean Ho, Jeanette K Stansborough, Jean-Michel Juliard, Jeanne Russo, Jeannette J M Schoep, Jeet Thambyrajah, Jeff Leimberger, Jeffery A Breall, Jeffrey A Kohn, Jeffrey C Milliken, Jeffrey Anderson, Jeffrey Blume, Jeffrey Kanters, Jeffrey Lorin, Jeffrey Moses, Jelena J Stepanovic, Jelena Celutkiene, Jelena Djokic, Jelena Stojkovic, Jenne M Jose, Jenne Manchery, Jennifer A Mull, Jennifer H Czerniak, Jennifer L Stanford, Jennifer Gillis, Jennifer Horst, Jennifer Isaacs, Jennifer Langdon, Jennifer Thomson, Jennifer Tomfohr, Jennifer White, Jen-Yuan Kuo, Jeremy Rautureau, Jerome Fleg, Jessica Berg, Jessica Rodriguez, Jessica Waldron, Jhina Patro, Jia Li, Jiajia Mao, Jiamin Liu, Jian'an Wang, Jianhua Li, Jianxin Zhang, Jie Qi, Jihyun Lyo, Jill Marcus, Jim Blankenship, Jing Zhang, Jingjing Liu, Jing-Yao Fan, Jiun-Yi Li, Jiwan Pradhan, Jiyan Chen, J M Rivera Caravaca, Jo Evans, Joan Garcia Picart, Joan Hecht, Joanna Jaroch, Joanna Zalewska, Joanne Kelly, Joanne Taaffe, João Reynaldo Abbud, João V Vitola, Joaquín V Peñafiel, Jocelyne Benatar, Jody Bindeman, Joe Sabik, Joel Klitch, Johann Christopher, Johannes Aspberg, John D Friedman, John F Beltrame, John F Heitner, John Joseph Graham, John R Davies, John Doan, John Kotter, John Kurian, John Mukai, John Pownall, Jolanta Sobolewska, Jon Kobashigawa, Jonathan L Goldberg, Jonathan W Bazeley, Jonathan Byrne, Jonathan Himmelfarb, Jonathan Leipsic, Jonean Thorsen, Jorge F Trejo Gutierrez, Jorge Escobedo, Jorik Timmer, José A Ortega-Ramírez, José Antonio Marin-Neto, Jose D Salas, Jose Enrique Castillo, Jose Francisco Saraiva, José J Cuenca-Castillo, Jose L Diez, José Luis Narro Villanueva, José Luiz da Vieira, José M Flores-Palacios, Jose Ramon Gonzalez, Jose Seijas Amigo, Jose Fragata, Josep Maria Padró, Josheph F X McGarvey Jr, Joseph Hannan, Joseph Sacco, Joseph Sweeny, Joseph Wiesel, Josephine D Abraham, Joshua P Loh, Joy Burkhardt, Joyce R White, Joyce Riestenberg-Smith, Judit Sebo, Judith L Meadows, Judith Wright, Judy Mae Foltz, Judy Hung, Judy Otis, Juergen Stumpf, Jui-Peng Tsai, Julia S Dionne, Julia de Aveiro Morata, Julie Bunke, Julie Morrow, Julio César Figal, Jun Fujita, Jun Jiang, Junhua Li, Junqing Yang, Juntima Euathrongchit, Jyotsna Garg, K Manjula Rani, K Preethi, Kaatje Goetschalckx, Kai Eggers, Kamalakar Surineni, Kanae Hirase, T R Kapilamoorthy, Karen Calfas, Karen Gratrix, Karen Hallett, Karen Hultberg, Karen Nugent, Karen Petrosyan, Karen Swan, Karolina Kryczka, Karolina Wojtczak-Soska, Karolina Wojtera, Karsten Lenk, Karthik Ramasamy, Katarzyna Łuczak, Katarzyna Malinowska, Kate Pointon, Kate Robb, Katherine Martin, Kathleen Claes, Kathryn Carruthers, Kathy E Siegel, Katia Drouin, Katie Fowler-Lehman, Kavita Rawat, Kay Rowe, Keiichi Fukuda, Keith A A Fox, Ken Mahaffey, Kendra Unterbrink, Kenneth Giedd, Kerrie Van Loo, Kerry Lee, Kerstin Bonin, Kevin R Bainey, Kevin T Harley, Kevin Anstrom, Kevin Chan, Kevin Croce, Kevin Landolfo, Kevin Marzo, Keyur Patel, Khaled Abdul-Nour, Khaled Alfakih, Khaled Dajani, Khaled Ziada, Khaula Baloch, Khrystyna Kushniriuk, Kian-Keong Poh, Kim F Ireland, Kim Holland, Kimberly Ann Byrne, Kimberly E Halverson, Kimberly Elmore, Kimberly Miller-Cox, Kiran Reddy, Kirsten J Quiles, Kirsty Abercrombie, Klaus Matschke, Konrad Szymczyk, Koo Hui Chan, Kotiboinna Preethi, Kozhaya Sokhon, Krissada Meemuk, Kristian Thygesen, Kristin M Salmi, Kristin Newby, Kristina Wippler, Kristine Arges, Kristine Teoh, Krystal Etherington, Krystyna Łoboz-Grudzień, Krzysztof W Reczuch, Krzysztof Bury, Krzysztof Drzymalski, Krzysztof Kukuła, Kuo-Tzu Sung, Kurt Huber, Ladda Douangvila, Lance Sullenberger, Larissa Miranda Trama, Laszlone Matics, Laura Drew, Laura Flint, Laura Keinaite, Laura Sarti, Laurel Kolakaluri, Lawrence M Phillips, Lawrence Friedman, Lawrence Phillips, Lazar Velicki, Leah Howell, Leandro C Maranan, Leanne Cox, Ledjalem Daba, Lei Zhang, Lekshmi Dharmarajan, Leo Bockeria, Leonardo Pizzol Caetano, Leonardo Bridi, Leonid L Bershtein, Leszek Sokalski, Li Hai Yan, Li Li, Lia Nijmeijer, Lidia Sousa, Lihong Xu, Lihua Zhang, Lili Zhang, Lilia Schiavi, Lilian Mazza Barbosa, Lillian L Khor, Lina Felix-Stern, Linda L Hall, Linda M Hollenweger, Linda Arcand, Linda Davidson-Ray, Linda Schwarz, Lindsey N Sikora, Lingping Chi, Lino Patricio, Liping Zhang, Lisa Chaytor, Lisa Hatch, Lisa McCloy, Lisa Wong, Liselotte Persson, Lixin Jiang, Liz Low, Ljiljana Pupic, Loïc Bière, Lorenzo Monti, Lori Christensen, Lori Pritchard, Loriane Black, Lori-Ann Desimone, Lori-Ann Larmand, Lorraine McGregor, Louise Morby, Louise Thomson, Luc Harvey, Luciana de Pádua Baptista, Lucilla Garcia, Ludivine Eliahou, Ludmila Helmer, Luis F Smidt, Luis Bernanrdes, Luis Guzman, Luiz A Carvalho, Luyang Xiong, Lynette L Teo, Lynn M Neeson, Lynne Winstanley, M Barbara Srichai-Parsia, M Quintana Giner, M Sowjanya Reddy, M Valdés Chávarri, M Grazia Rossi, Maarten Simoons, Maayan Konigstein, Maciej Lesiak, Maciej Olsowka, Mafalda Selas, Magalie Corfias, Magdalena Madero Rovalo, Magdalena Łanocha, Magdalena Miller, Magdalena Misztal-Teodorczyk, Magdalena Rantinella, Magdy Abdelhamid, Magnolia Jimenez, Mahboob Alam, Mahevamma Mylarappa, Mahfouz El Shahawy, Mahmoud Mohamed, Mahmud Al-Bustami, Majo X Joseph, Malgorzata Frach, Małgorzta Celińska-Spodar, Malte Helm, Manas Chacko, Mandy Murphy, Manitha Vinod, Manjula Rani, Manu Dhawan, Manuela Mombelli, Marcel Weber, Marcello Galvani, Marcelo Jamus Rodrigues, Marcia F Dubin, Marcia F Werner Bayer, Marcin Szkopiak, Marco Antonio Monsalve, Marco Bizzaro Santos, Marco Magnoni, Marco Marini, Marco Sicuro, Marco Zenati, Marcos Valério Coimbra Resende, Marek Roik, Margalit Bentzvi, Margaret Gilsenan, Margaret Iraola, Margot C 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Jr, Matthew Budoff, Matthew Jezior, Matthew Luckie, Matthias Friedrich, Mauren P Haeffner, Maximilian Tscharre, Max-Paul Winter, Mayana Almeida, Mayil S Krishnam, Mayuri Patel, Meenakshi Mishra, Megan Manocchia, Meghana Kakade, Melanie J Munro, Melissa D Chaplin, Melissa LeFevre, Mervyn Andiapen, Michael A Gibson, Michael B Rubens, Michael C Turner, Michael D Shapiro, Michael W Lee, Michael Berlowitz, Michael Davidson, Michael Mack, Michael McDaniel, Michael Mumma, Michal Wlodarczyk, Michel G Khouri, Michel S Slama, Michele Rawlins, Michelle M Bonner, Michelle M Seib, Michelle Chang, Michelle Crowder, Michelle Dixon, Michelle Mayon, Michelle McEvoy, Michelle Yee, Miguel M Fernandes, Miguel Nobre Menezes, Miguel Souto Bayarri, Miguel Barrero, Mikhail T Torosoff, Milan R Dobric, Milan Dobric, Milica Nikola Dekleva, Milind Avdhoot Gadkari, Millie Gomez, Min Tun Kyaw, Miriam Brooks, Miroslav Stevo Martinovic, Mitchel B Lustre, Mohammad Tariq Vakani, Mohammad El-Hajjar, Mohammed Al-Amoodi, Mohammed Hussain, Mohammed Saleem, Moisés Blanco-Calvo, Moisés Jiménez-Santos, Mona Bhatia, Monica Rosca, Monika Laukyte, Montserrat Gracida Blanca, Montserrat Vila Perales, Mouaz H Al-Mallah, Moysés de Oliveira Filho, Mpiko Ntsekhe, Muhamed Saric, Mulei Chen, Myriam Brousseau, Myrthes Emy Takiuti, Nada Cemerlic-Adjic, Nadia Asif, Nadia Gakou, Nafisa Hussain, Nana O Katamadze, Nancy L Clapp, Nancy Aedy, Nandita Nataraj, Nanette K Wenger, Naomi Uchida, Nasrul Ismail, Natalia S Oliveira, Natalia de Carvalho Maffei, Natalie Spitzer, Natasha C Putnam, Naved Aslam, Neamat Mowafy, Neeraj Pandit, Neeraj Parakh, Nevena Garcevic, Ngaire Meadows, Nhi N Tran, Nicholas Danchin, Nicki Lakeman, Nicola Johnston, Nicolas W Shammas, Nicole Saint Vrestil, Nicole Deming, Nier Zhong, Niket Patel, Nikola N Boskovic, Nikolaos Karogiannis, Nikos Werner, Nina Johnston, Ning Zhang, Ning Zhou, Niree Hindoyan, Nirmal Kumar, Nitika Chadha, Nitish Naik, Nodira Aripova, Noloyiso Mtana, Nona A Eskelson, Noor Syamira 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Paula García-González, Paulo Cury Rezende, Paulo Ricardo Caramori, Pavel S Kozlov, Pedro Canas Silva, Pedro Gabriel Melo Barros E Silva, Pedro Píccaro de Oliveira, Pedro Carvalho, Pedro Modas, Pedro Rio, Peeyush Jain, Peiyu He, Peter A McCullough, Peter H Stone, Peter M Pollak, Peter Douglass, Peter Henriksen, Peter OKane, Peter Ong, Philip Jones, Philip Rogal, Philippe Généreux, Philippe Menasche, Philippe Rheault, Phoebe Goold, Pierre Gervais, Pierre Michaud, Pilar Calvillo, Ping Chai, Piotr Jakubowski, Piotr Pruszczyk, Piotr Slomka, Piyamitr Sritara, Poay-Huan Loh, Poonam Sonawane, Pouneh Samadi, Pragnesh P Parikh, Prakash Deedwania, Pranav M Patel, Praneeth Polamuri, Pratiksha Sharma, Precilia Vasquez, Preeti Kamath, Prince Thomas, Priyadarshani Arambam, Puja K Mehta, Purvez Grant, Pushpa Naik, Qi Zhong, Qian Zhao, Qiang Zhou, Qianqian Yuan, Qin Yu, Qingxian Li, Qiulan Xie, Qiutang Zeng, R J Vindhya, R James Gerlach, Rachel King, Rada Vučić, Radmila Lyubarova, Radoslaw Pracon, 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Stephanie M Lane, Stephanie Ferket, Stephanie Kelly, Stephanie Wasmiller, Stephen H McKellar, Stephen P Hoole, Stephen Fremes, Stephen Preston, Steve Leung, Steven A Fein, Steven J Lindsay, Steven P Sedlis, Steven Giovannone, Steven Michael, Steven Weitz, Stijn van Vugt, Subhash Banerjee, Sudhir Naik, Suellen Hosino, Sukie Desire, Sukit Yamwong, Suku T Thambar, Sulagna Mookherjee, Suman Singh, Sundeep Mishra, Sunil Kumar Verma, Supap Kulthawong, Supatchara Khwakhong, Surendra Naik, Suresh Babu, Surin Woragidpoonpol, Suryaprakash Narayanappa, Susan Derbyshire, Susan Gent, Susan Mathus, Susan Milbrandt, Susan Moore, Susan Regan, Susan Stinson, Susan Webber, Susana Silva, Susanna Stevens, Susanne Gruensfelder, Suthara Aramcharoen, Suvarna Kolhe, Suzana Tavares, Suzanne Arnold, Suzanne Welsh, Svetlana Apostolovic, Swapna Kunhunny, Ta-Chuan Hung, Taissa Zappernick, Tali Sharir, Talita Silva, Tamara Colaiácovo Soares, Tapan Umesh Pillay, Tarun K Mittal, Tatiana Trifonova, Tauane Bello Duarte, Tauqir Huk, Téodora Dutoiu, Terrance Chua, Terry Weyand, Thabitha Charles, Theodoros Kofidis, Theresa McCreary, Thierry Lefevre, Thippeekaa Arumairajah, Thitipong Tepsuwan, Thomas J Mulhearn, Thomas M Meyer, Thomas P Rocco, Thomas R Downes, Thomas Crain, Thomas Haldis, Thomas Mathew, Thomas Redick, Thounaojam Indira Devi, Thuraia Nageh, Tia Cauthren, Tiago Silva, Tiffany Little, Tijana Andric, Tina Harding, Titus Lau, Tiziana Formisano, Tiziano Moccetti, Tomasz Ciurus, Tomasz Mazurek, Tomasz Tarchalski, Toshiyuki Nagai, Tri Tran, Tricia Youn, Trish Tucker, Trudie Milner, Tuhina Bose, Tushar Kotecha, Udo Sechtem, Uma S Valeti, Umberto Cucchini, Umesh Badami, Upendra Kaul, V K Bahl, V S Narain, Valentina Casali, Valeria Godoy, Valerie Robesyn, Vamshi P Priya, Vandana Yadav, Vera McKinney, Veronica De Lenges, Veronica Tinnirello, Vicente Miro, Victor Navarro, Victoria Gumerova, Victoria Hernandez, Vidya Seeratan, Vijay Kumar, Vikentiy Y Kozulin, Viktoria Bulkley, Vilmar Veiga Jr, Vincent Setang, C P Vineeth, Virginai Pubull Nuñez, Virginia Fernández-Figares, Vitor Gomes, Viviana Gabriel, Viviane Dos Santos, Viviane Almeida, Vlad A Iliescu, Vladan Mudrenovic, Vladimir Dzavik, Vojislav L Giga, Walter Enrique Mogrovejo, Wan Xian Chan, Wanda C Marfori, Wanda Parker, Warangkana Mekara, Wassim Nona, Wayne Old, Wayne Pennachi, Weerachai Nawarawong, Wei Chen, Wei Su, Weibing Xing, Wei-Ren Lan, Wenda Crawford, Wendy L Stewart, Wendy Drewes, Wenhua Lin, William B Abernethy, William D Salerno, William F Fearon, William Vergoni, William Weintraub, Winnie C Sia, Wlodzimierz J Musial, Xacobe Flores-Ríos, Xavier Garcia-Moll Marimon, Xi Su, Xiang Ma, Xiangqiong Gu, Xiao Wang, Xiaomei Li, Xiaowei Yao, Xin Fu, Xin Su, Xin Zeng, Xinchun Yang, Xiuhong Li, Xuehua Fang, Xutong Wang, Yaming Geng, Yan Yan, Yanek Pépin-Dubois, Yanfu Wang, Yang Wang, Yanmeng Tian, Yaping Huang, Yechen Han, Yesenia Zambrano, Yi-Hsuan Yang, Ying Tung Sia, Yining Yang, Yitong Ma, Yolayfi Peralta, Yongjian Wu, Yu Kunwu, Yu Zhao, Yudong Peng, Yueh-Hung Lin, Yulan Zhao, Yumei Dong, Yunhai Zhao, Yutthaphan Wannasopha, Yvonne Taul, Zakir Sahul, Zalina Kudzoeva, Zbigniew Kalarus, Zeljko Z Markovic, Zhen Huang, Zheng Ji, Zhenyu Liu, Zhou Yue, Zhulin Zhang, Zhuxi Li, Zile Singh Meharwal, Ziliang Bai, Zixiang Yu, Zohra Huda, Zoltan Davidovits

Subjects
Male, Cardiac Catheterization, Computed Tomography Angiography, medicine.medical_treatment, Myocardial Ischemia, Coronary Disease, Coronary Artery Disease, Kaplan-Meier Estimate, 030204 cardiovascular system & hematology, Coronary Angiography, ISCHEMIA Research Group, law.invention, Angina, Coronary artery disease, 0302 clinical medicine, Randomized controlled trial, law, Cardiovascular Disease, Myocardial Revascularization, 030212 general & internal medicine, Coronary Artery Bypass, 11 Medical and Health Sciences, Cardiac catheterization, General Medicine, Middle Aged, humanities, Cardiovascular Diseases, Cardiology, Female, Human, medicine.medical_specialty, Ischemia, Article, 03 medical and health sciences, Geriatric cardiology, Percutaneous Coronary Intervention, General & Internal Medicine, Internal medicine, medicine, Humans, Angina, Unstable, Aged, business.industry, Coronary Artery Bypa, Percutaneous coronary intervention, Bayes Theorem, medicine.disease, Heart failure, Quality of Life, business
Abstract

BACKGROUND: Among patients with stable coronary disease and moderate or severe ischemia, whether clinical outcomes are better in those who receive an invasive intervention plus medical therapy than in those who receive medical therapy alone is uncertain. METHODS: We randomly assigned 5179 patients with moderate or severe ischemia to an initial invasive strategy (angiography and revascularization when feasible) and medical therapy or to an initial conservative strategy of medical therapy alone and angiography if medical therapy failed. The primary outcome was a composite of death from cardiovascular causes, myocardial infarction, or hospitalization for unstable angina, heart failure, or resuscitated cardiac arrest. A key secondary outcome was death from cardiovascular causes or myocardial infarction. RESULTS: Over a median of 3.2 years, 318 primary outcome events occurred in the invasive-strategy group and 352 occurred in the conservative-strategy group. At 6 months, the cumulative event rate was 5.3% in the invasive-strategy group and 3.4% in the conservative-strategy group (difference, 1.9 percentage points; 95% confidence interval [CI], 0.8 to 3.0); at 5 years, the cumulative event rate was 16.4% and 18.2%, respectively (difference, -1.8 percentage points; 95% CI, -4.7 to 1.0). Results were similar with respect to the key secondary outcome. The incidence of the primary outcome was sensitive to the definition of myocardial infarction; a secondary analysis yielded more procedural myocardial infarctions of uncertain clinical importance. There were 145 deaths in the invasive-strategy group and 144 deaths in the conservative-strategy group (hazard ratio, 1.05; 95% CI, 0.83 to 1.32). CONCLUSIONS: Among patients with stable coronary disease and moderate or severe ischemia, we did not find evidence that an initial invasive strategy, as compared with an initial conservative strategy, reduced the risk of ischemic cardiovascular events or death from any cause over a median of 3.2 years. The trial findings were sensitive to the definition of myocardial infarction that was used. (Funded by the National Heart, Lung, and Blood Institute and others; ISCHEMIA ClinicalTrials.gov number, NCT01471522.).

Published
2020

17. Vaccinia-related kinase 2 drives pancreatic cancer progression by protecting Plk1 from Chfr-mediated degradation

Author

Hengqing Zhu, Qing Li, Hong Peng, Bin Xu, Zhaoxia Zeng, Zi Jiang, Liangyun Guo, Sisi Chen, Yulan Zhao, and Jing Zhu

Subjects
0301 basic medicine, Cancer Research, biology, Kinase, Complement factor I, PLK1, Ubiquitin ligase, Cell biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Ubiquitin, 030220 oncology & carcinogenesis, CHFR, Genetics, biology.protein, Phosphorylation, Threonine, Molecular Biology
Abstract

As a key cell cycle regulator, polo-like kinase 1 (Plk1) has been recognized as a crucial factor involved in the progression of pancreatic cancer (PC). However, its regulatory mechanism is poorly understood. Here, we present evidence that Plk1 is a novel substrate of vaccinia-related kinase 2 (VRK2), a serine-threonine kinase that is highly expressed and predicts poor prognosis in PC. VRK2 phosphorylates Plk1 at threonine 210 and protects it from ubiquitin-dependent proteasomal degradation. We showed that mechanistically complement factor H-related protein (CFHR), as a major E3 ligase, promotes Plk1 degradation by ubiquitinating it at lysine 209. Phosphorylation of Plk1 at threonine 210 by VRK2 interferes with the interaction of Chfr with Plk1 and antagonizes Plk1 ubiquitination, thereby stabilizing the Plk1 protein. Taken together, our data reveal a mechanism of Plk1 overexpression in PC and provide evidence for targeting VRK2 as a potential therapeutic strategy.

Published
2021

18. Integrated Analysis of Tumor Mutation Burden and Immune Infiltrates in Hepatocellular Carcinoma

Author

Yulan Zhao, Ting Huang, and Pintong Huang

Subjects
Clinical Biochemistry, hepatocellular carcinoma, tumor mutation burden, immune cells, The Cancer Genome Atlas, CIBERSORT
Abstract

Tumor mutation burdens (TMBs) act as an indicator of immunotherapeutic responsiveness in various tumors. However, the relationship between TMBs and immune cell infiltrates in hepatocellular carcinoma (HCC) is still obscure. The present study aimed to explore the potential diagnostic markers of TMBs for HCC and analyze the role of immune cell infiltration in this pathology. We used OA datasets from The Cancer Genome Atlas database. First, the “maftools” package was used to screen the highest mutation frequency in all samples. R software was used to identify differentially expressed genes (DEGs) according to mutation frequency and perform functional correlation analysis. Then, the gene ontology (GO) enrichment analysis was performed with “clusterProfiler”, “enrichplot”, and “ggplot2” packages. Finally, the correlations between diagnostic markers and infiltrating immune cells were analyzed, and CIBERSORT was used to evaluate the infiltration of immune cells in HCC tissues. As a result, we identified a total of 359 DEGs in this study. These DEGs may affect HCC prognosis by regulating fatty acid metabolism, hypoxia, and the P53 pathway. The top 15 genes were selected as the hub genes through PPI network analysis. SRSF1, SNRPA1, and SRSF3 showed strong similarities in biological effects, NCBP2 was demonstrated as a diagnostic marker of HCC, and high NCBP2 expression was significantly correlated with poor over survival (OS) in HCC. In addition, NCBP2 expression was correlated with the infiltration of B cells (r = 0.364, p = 3.30 × 10−12), CD8+ T cells (r = 0.295, p = 2.71 × 10−8), CD4+ T cells, (r = 0.484, p = 1.37 × 10−21), macrophages (r = 0.551, p = 1.97 × 10−28), neutrophils (r = 0.457, p = 3.26 × 10−19), and dendritic cells (r = 0.453, p = 1.97 × 10−18). Immune cell infiltration analysis revealed that the degree of central memory T-cell (Tcm) infiltration may be correlated with the HCC process. In conclusion, NCBP2 can be used as diagnostic markers of HCC, and immune cell infiltration plays an important role in the occurrence and progression of HCC.

Published
2022

21. Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature

Author

Changming Zhou, Xiaona Gao, Xianhong Cao, Guanming Tian, Cheng Huang, Lianying Guo, Yulan Zhao, Guoliang Hu, Ping Liu, and Xiaoquan Guo

Subjects
Nutrition and Dietetics, Endocrinology, Diabetes and Metabolism, Food Science
Abstract

Emerging evidence has revealed the dysbiosis of gut microbiota contributes to development of metabolic diseases in animals. However, the potential interaction between gut microbiota and host metabolism in growing hens under metabolic disorder induced by chronic heat exposure (CHE) remains inconclusive. The aim of our study was to examine the potential association among the cecal microbiota community, physiological indicators, and serum metabolite profiles in CHE hens. One hundred and eighty Hy-Line Brown hens were randomly allocated into three groups: thermoneutral control (TN), heat stress (HS), and pair-fed (PF). The experiment lasted for 5 weeks, with the first 2 weeks serving as the adaptation period. Results showed that the expression level of heat shock protein 70 (HSP70) in both serum and cecal tissues was significantly increased in the HS group. Serum parameters analysis also revealed that CHE caused physiological function damage and metabolic disorders. These results suggest the experiment was successful, inducing chronic heat stress. 16S rRNA sequencing analysis showed that the CHE can clearly induce dysbiosis of the gut microbial community reflected in the increment of the F/B ratio. Besides, serum untargeted metabolomics revealed the relative concentrations of 40 metabolites were significantly altered in the HS group compared with the TN group. Pathway analysis showed that these metabolites were mainly involving the increased proteolysis rather than lipolysis, and this tendency could be a specific metabolic adaptation of the poultry. The pair-feed experiment showed that the above changes induced by CHE were partly independent from the reduction of feed intake. Mantel correlation analysis between gut microorganisms and physiological indicators showed that the phylum Firmicutes and Euryarchaeota have a potential interaction with a serum lipid parameter. Random forest analysis showed that both genus Faecalibacterium and Methanobrevibacter were important predictors of the CHE-induced lipid metabolism disorder. Taken together, our findings may contribute to a better understanding of the metabolic mechanisms underlying the energy metabolism imbalance caused by the CHE and provide novel insights into the host-microbes interactions and its effects on the metabolic adaptation of hens under chronic heat exposure.

Published
2022

22. Exposed to Mercury-Induced Oxidative Stress, Changes of Intestinal Microflora, and Association between them in Mice

Author

Guyue Li, Yulan Zhao, Yu Zhuang, Caiying Zhang, Fan Yang, Changming Zhou, Guoliang Hu, Xiaoquan Guo, Chonghong Xing, Lin Li, Ping Liu, and Huabin Cao

Subjects
Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, chemistry.chemical_element, 010501 environmental sciences, medicine.disease_cause, 01 natural sciences, Biochemistry, Inorganic Chemistry, Andrology, Superoxide dismutase, Mice, 03 medical and health sciences, Cecum, chemistry.chemical_compound, Malondialdehyde, Lactobacillus, medicine, Animals, 0105 earth and related environmental sciences, chemistry.chemical_classification, Glutathione Peroxidase, 0303 health sciences, biology, Superoxide Dismutase, Glutathione peroxidase, 030302 biochemistry & molecular biology, Biochemistry (medical), Mercury, General Medicine, Glutathione, biology.organism_classification, Gastrointestinal Microbiome, Mercury (element), Oxidative Stress, medicine.anatomical_structure, chemistry, biology.protein, Oxidative stress
Abstract

Twelve Kunming mice were randomly divided into two groups (n = 6), and administered with distilled water containing 0 mg/L and 160 mg/L HgCl2 respectively, with an experimental period of 3 days. Our results showed that mercury exposure significantly reduced weight gain in mice (P

Published
2020

23. A Heuristic Transferring Strategy for Heterogeneous-Cached ICN

Author

Yulan Zhao and Jianhui Lv

Subjects
ACO, General Computer Science, transferring strategy, Computer science, Heuristic (computer science), Distributed computing, Ant colony optimization algorithms, General Engineering, Throughput, Energy consumption, Network topology, Heterogeneous ICN, expectation efficiency, Campus network, Knapsack problem, General Materials Science, Network performance, KP01, lcsh:Electrical engineering. Electronics. Nuclear engineering, Cache, lcsh:TK1-9971, Throughput (business)
Abstract

The in-network caching is a considerably significant feature of Information-Centric Networking (ICN), especially the heterogeneous-cached ICN has been widely investigated since it accords with the actual network deployment. For the heterogeneous-cached ICN, although there have been many proposals to improve network performance, it is very difficult for these approaches to reach the optimal network performance with multiple metrics consideration. Therefore, in this paper, we propose a heuristic transferring strategy which selects some Content Routers (CRs) and combines them to facilitate the optimal network performance under a constrained total cache budget. At first, we quantify energy consumption, CR load, cache hit ratio and throughput, because the optimal network performance depends on four objects, i.e., minimizing energy consumption and CRs load as well as maximizing cache hit ratio and throughput. Then, based on the given network constraints and objects, we convert the CR transferring problem into 0-1 Knapsack Problem (KP01). Finally, in order to effectively obtain the optimal solution, we propose a heuristic approach based on Ant Colony Optimization (ACO) and expectation efficiency to solve KP01. The simulation is driven by the real YouTube dataset from campus network measurement over GTS and Deltacom topologies, and the experimental results demonstrate that the proposed strategy is more efficient compared to three baselines.

Published
2020

24. Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects

Author

Li Li, Lu Hongwei, Jiangming Luo, Shenfang Zha, Xinyun Han, Li Yang, Peixin Chen, Shuang Wan, Qingyi He, Qijie Dai, Zhengwei Yang, Pingping Ma, Junxian Hu, Yohanes Kristo Sugiarto Utomo, Yulan Zhao, Wanqian Liu, and Xiaorui Jiang

Subjects
Male, Artificial bone, Bone Regeneration, Materials science, Nanofibers, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Rats, Sprague-Dawley, Vasculogenesis, In vivo, Cell Adhesion, Animals, Humans, General Materials Science, Progenitor cell, Bone regeneration, Cell Proliferation, Endothelial Progenitor Cells, Neovascularization, Pathologic, Tissue Engineering, Tissue Scaffolds, Regeneration (biology), Skull, 021001 nanoscience & nanotechnology, In vitro, Rats, 0104 chemical sciences, Cell biology, Transplantation, embryonic structures, cardiovascular system, Bone Diseases, Peptides, 0210 nano-technology, circulatory and respiratory physiology
Abstract

Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.

Published
2019

25. Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus

Author

Siyuan Liu, Yuying Liao, Yalan Yang, Dagang Tao, Peng Yang, Shouzhang Sun, Yonggang Liu, Yijie Tang, Zhonglin Tang, Shengsong Xie, Bin Chen, and Yulan Zhao

Subjects
viruses, animal diseases, Biomedical Engineering, Recombinase Polymerase Amplification, DNA, Single-Stranded, Real-Time Polymerase Chain Reaction, Biochemistry, Genetics and Molecular Biology (miscellaneous), Virus, Fluorescence, Genes, Reporter, Limit of Detection, CRISPR, Porcine respiratory and reproductive syndrome virus, Polymerase, biology, Reverse Transcriptase Polymerase Chain Reaction, virus diseases, General Medicine, respiratory system, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, Virology, Highly sensitive, Reverse transcription polymerase chain reaction, biology.protein, RNA, Viral, CRISPR-Cas Systems
Abstract

Porcine reproductive and respiratory syndrome (PRRS) is an economically important disease of swine that is caused by PRRS virus (PRRSV). In this study, we established a fluorescence assay for highly sensitive detection of PRRSV through integration of the reverse transcription-recombinase polymerase amplification (RT-RPA)-coupled Cas12a system with an optical property of single stranded DNA-fluorescently quenched (ssDNA-FQ) reporter. This technique can achieve isothermal and visual detection of PRRSV in 25 min. In particular, the assay reaction can be completed in a single tube. The limit of sensitivity for PRRSV detection was single copy without cross-reactivity of other porcine viruses. Correlation between 11 PRRSV clinical samples measured by the quantitative reverse transcription polymerase chain reaction (RT-qPCR) and CRISPR/Cas12a assay was determined; the result showed that our results were highly accurate. To sum up, this study developed a visual, sensitive, and specific method of nucleic acid detection based on a CRISPR-Cas12a technique for the on-site detection of PRRSV.

Published
2021

26. Age‐related changes in metabolites in young donor livers and old recipient sera after liver transplantation from young to old rats

Author

Qunhua Han, Zhuo Xian Meng, Hui Li, Yulan Zhao, Mangli Zhang, Lin Wang, Qin Zhang, Mengyuan Jia, Yunmei Yang, Lijun Zhu, and Jimin Shao

Subjects
0301 basic medicine, Aging, medicine.medical_treatment, Physiology, Biology, Liver transplantation, Transcriptome, transcriptomics, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Metabolomics, orthotopic liver transplantation, Living Donors, medicine, Animals, metabolic pathways, Original Paper, Age Factors, Original Articles, Cell Biology, Metabolism, Liver Transplantation, Rats, untargeted metabolomics, Metabolic pathway, 030104 developmental biology, Liver, chemistry, Ageing, Arachidonic acid, Liver function, 030217 neurology & neurosurgery, liver ageing
Abstract

Liver ageing not only damages liver function but also harms systemic metabolism. To better understand the mechanisms underlying liver ageing, we transplanted the livers of young rats to young and old rats and performed untargeted metabolomics to detect changes in the metabolites in the liver tissues and sera. A total of 153 metabolites in the livers and 83 metabolites in the sera were different between the old and young rats that did not undergo liver transplantation; among these metabolites, 7 different metabolites were observed in both the livers and sera. Five weeks after liver transplantation, the levels of 25 metabolites in the young donor livers were similar to those in the old rats, and this result probably occurred due to the effect of the whole‐body environment of the older recipients on the young livers. The 25 altered metabolites included organic acids and derivatives, lipids and lipid‐like molecules, etc. In the sera, the differences in 78 metabolites, which were significant between the young and old rats, were insignificant in the old recipient rats and made the metabolic profile of the old recipients more similar to that of the young recipients. Finally, combining the above metabolomic data with the transcriptomic data from the GEO, we found that the altered metabolites and genes in the liver were enriched in 9 metabolic pathways, including glycerophospholipid, arachidonic acid, histidine and linoleate. Thus, this study revealed important age‐related metabolites and potential pathways as well as the interaction between the liver and the whole‐body environment., Aging is related to metabolic disorder. To better understand the underlying mechanisms, we transplanted livers of young rats to young and old rats. Analyses of differential metabolites in the livers and sera revealed that while the young donor livers underwent aging in the old recipients, they could benefit the old recipients through circulation. Integrated metabolomic and transcriptomic profiles suggested altered metabolic pathways during liver aging.

Published
2021

27. Research on PEMFC Internal Temperature Predictions and Thermal Management Strategy Based on a Kalman Algorithm

Author

Yongqi Wei, Haitao Yun, and Yulan Zhao

Subjects
Materials science, Renewable Energy, Sustainability and the Environment, 020209 energy, Nuclear engineering, Energy Engineering and Power Technology, Proton exchange membrane fuel cell, 02 engineering and technology, Thermal management of electronic devices and systems, 021001 nanoscience & nanotechnology, Internal temperature, Nuclear Energy and Engineering, Kalman algorithm, 0202 electrical engineering, electronic engineering, information engineering, Temperature difference, 0210 nano-technology, Waste Management and Disposal, Civil and Structural Engineering
Abstract

The study addresses proton exchange membrane fuel cell (PEMFC) internal temperature predictions and the designed thermal management strategy based on the predicted temperature. The effect o...

Published
2021

28. USP22 Deubiquitinates CD274 to Suppress Anticancer Immunity

Author

Xing Huang, Yulan Zhao, Xinyu Zhao, Yu Lou, Junli Wang, Shanshan Li, Xueli Bai, Tingbo Liang, Qi Zhang, Lin Wang, Qi Chen, and Xiaozhen Zhang

Subjects
0301 basic medicine, Cancer Research, medicine.medical_treatment, Immunology, Biology, B7-H1 Antigen, Mice, 03 medical and health sciences, Lymphocytes, Tumor-Infiltrating, 0302 clinical medicine, Immune system, Palmitoylation, Mice, Inbred NOD, Cell Line, Tumor, medicine, Animals, Humans, Ubiquitins, Immunogenicity, Liver Neoplasms, Cancer, Immunotherapy, Prognosis, medicine.disease, Xenograft Model Antitumor Assays, Research Highlight, Mice, Inbred C57BL, Survival Rate, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, Phosphorylation, Female, Liver cancer, Protein Processing, Post-Translational, Ubiquitin Thiolesterase, Deubiquitination
Abstract

PD-1 (CD279)–PD-L1 (CD274) inhibitory signaling is critical for cancer immune evasion, and thus has become one of the major targets in anticancer immunotherapy. There are several studies that demonstrate the potent effects of posttranslational modifications of CD274 on immune inactivation and suppression, such as ubiquitination, phosphorylation, glycosylation, and palmitoylation. However, the regulatory mechanisms for CD274 deubiquitination are still largely unclear. Here, we identified ubiquitin-specific protease 22 (USP22) as a novel deubiquitinase of CD274. USP22 directly interacted with the C terminus of CD274, inducing its deubiquitination and stabilization. Across multiple cancer types, USP22 was highly expressed and frequently altered in liver cancer, closely correlating with poor prognosis of these patients. Genetic depletion of USP22 inhibited liver cancer growth in an immune system–dependent manner, increased tumor immunogenicity and tumor-infiltrating lymphocytes, and improved therapeutic efficacy of CD274-targeted immunotherapy and CDDP-based chemotherapy in mice. We demonstrate that targeting USP22 is a promising strategy to potentiate anticancer immunity for CD274-amplified cancer.

Published
2019

31. Vaccinia-related kinase 2 drives pancreatic cancer progression by protecting Plk1 from Chfr-mediated degradation

Author

Hengqing, Zhu, Qing, Li, Yulan, Zhao, Hong, Peng, Liangyun, Guo, Jing, Zhu, Zi, Jiang, Zhaoxia, Zeng, Bin, Xu, and Sisi, Chen

Subjects
Pancreatic Neoplasms, Proto-Oncogene Proteins, Vaccinia, Cell Cycle Proteins, Protein Serine-Threonine Kinases, Pancreas
Abstract

As a key cell cycle regulator, polo-like kinase 1 (Plk1) has been recognized as a crucial factor involved in the progression of pancreatic cancer (PC). However, its regulatory mechanism is poorly understood. Here, we present evidence that Plk1 is a novel substrate of vaccinia-related kinase 2 (VRK2), a serine-threonine kinase that is highly expressed and predicts poor prognosis in PC. VRK2 phosphorylates Plk1 at threonine 210 and protects it from ubiquitin-dependent proteasomal degradation. We showed that mechanistically complement factor H-related protein (CFHR), as a major E3 ligase, promotes Plk1 degradation by ubiquitinating it at lysine 209. Phosphorylation of Plk1 at threonine 210 by VRK2 interferes with the interaction of Chfr with Plk1 and antagonizes Plk1 ubiquitination, thereby stabilizing the Plk1 protein. Taken together, our data reveal a mechanism of Plk1 overexpression in PC and provide evidence for targeting VRK2 as a potential therapeutic strategy.

Published
2021

32. Microarray Analysis for Differentially Expressed Genes Between Stromal and Epithelial Cells in Development and Metastasis of Invasive Breast Cancer

Author

Rong Wang, Yulan Zhao, Ling Yin, Di Zhao, Jinbin Li, and Lei Fu

Subjects
Stromal cell, Tumor suppressor gene, Breast Neoplasms, Biology, medicine.disease_cause, Metastasis, Breast cancer, Genetics, medicine, Humans, Gene Regulatory Networks, Protein Interaction Maps, KEGG, skin and connective tissue diseases, Molecular Biology, Oligonucleotide Array Sequence Analysis, Cancer, Epithelial Cells, medicine.disease, Gene expression profiling, Gene Expression Regulation, Neoplastic, Computational Mathematics, Gene Ontology, Computational Theory and Mathematics, Modeling and Simulation, Cancer research, Female, Stromal Cells, Carcinogenesis
Abstract

Both epithelium and stroma are involved in breast cancer invasion and metastasis. This study aimed at identifying the roles of the stroma in breast cancer tumorigenesis and metastasis. Gene expression profiling GSE10797 was downloaded from the Gene Expression Omnibus database, and it included 28-paired stroma and epithelium breast tissue samples from invasive breast cancer patients and 10 paired normal breast tissue samples. Differentially expressed genes (DEGs) between breast cancer and normal breast tissue samples were identified by using the limma package followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to seek the potential functions of DEGs. Moreover, a protein-protein interaction network was constructed based on the String database, and modules were selected through the BioNet tool. Further, functional annotations of DEGs were carried out by using tumor suppressor gene and tumor associated gene databases. Ultimately, KEGG pathway enrichment analysis for DEGs in modules was performed. A total of 38 and 156 DEGs were identified from normal invasive stromal cells and epithelial cells, respectively. DEGs in stromal and epithelial cells were significantly enriched in extracellular matrix (ECM)- and cell proliferation-related functions. COL1A2, a hub node in the stromal module, was mainly enriched in ECM-receptor interaction and focal adhesion pathways. JUN, a hub node in the epithelium module, was significantly enriched in cancer and ErbB signaling pathways. COL1A2, COL1A1, COL3A1, JUN, and FN1 might be vital for tumorigenesis and metastasis of invasive breast cancer. These genes might be potential therapeutic targets for breast cancer treatment.

Published
2020

33. Subchronic oral mercury caused intestinal injury and changed gut microbiota in mice

Author

Xiaoquan Guo, Zheng Xu, Yulan Zhao, Huabin Cao, Vincent Latigo, Cong Wu, Changming Zhou, Ping Liu, Guyue Li, Pei Liu, Lin Li, Sufang Cheng, Guoliang Hu, and Qingpeng Wu

Subjects
medicine.medical_specialty, Environmental Engineering, food.ingredient, 010504 meteorology & atmospheric sciences, 010501 environmental sciences, Gut flora, 01 natural sciences, Coprococcus, Mice, food, Internal medicine, medicine, Environmental Chemistry, Animals, Helicobacter, Waste Management and Disposal, Gene, Salinicoccus, 0105 earth and related environmental sciences, biology, Microbiota, Metabolic disorder, Mercury, biology.organism_classification, medicine.disease, Pollution, Gastrointestinal Microbiome, Intestinal Diseases, Endocrinology, Apoptosis, Dysbiosis
Abstract

Mercury is a key global pollutant, yet the mechanism by which mercury-exposure causes intestinal injury is not clear, we aimed to investigate the mechanism of intestinal injury and gut microbiota changes caused by mercury-exposure. Twelve Kunming mice were divided into two groups (n = 6), and the two groups were treated with 0 mg/L and 80 mg/L HgCl2 in drinking water for 90 days respectively. Our results showed that mercury-exposure prominently effected body weight gain and glucose levels. The mercury-exposed mice showed intestinal injury, which was diagnosed by Histopathological Examination and Transmission Electron Microscopy. Meanwhile, RT-PCR indicated that mercury-exposure significantly increased the expression of pro-apoptotic genes including Bax, JNK, ASK1, caspase3 and TNF-α, and significantly decreased the expression of the anti-apoptotic gene Bcl-2. Furthermore, high-throughput sequencing analysis showed that at the genus level some microbial populations including Coprococcus, Oscillospira and Helicobacter were significantly increased whereas some microbial populations including Lgnatzschineria, Salinicoccus and Bacillus were significantly decreased. Moreover, PICRUSt analysis revealed potential metabolic changes. Correlation analysis indicated that microorganisms were significantly correlated with apoptotic gene expression. In summary, our results indicated that mercury-exposure affected the growth and development of mice, induced intestinal microbiota dysbiosis and metabolic disorder, and aggravated apoptosis in mice.

Published
2020

34. Correlation between acute brain injury and brain metabonomics in dichlorvos-poisoned broilers

Author

Yu Zhuang, Ping Liu, Caiying Zhang, Huabin Cao, Xin Liu, Xiaoquan Guo, Cheng Huang, Cong Wu, Zheng Xu, Lujia Huang, Guyue Li, Sufang Cheng, Yulan Zhao, Guoliang Hu, Pei Liu, and Changming Zhou

Subjects
Environmental Engineering, Health, Toxicology and Mutagenesis, Pharmacology, medicine.disease_cause, Poisons, chemistry.chemical_compound, Dichlorvos, medicine, Animals, Metabolomics, Environmental Chemistry, Acetylcarnitine, Waste Management and Disposal, Hypoxanthine, Glial fibrillary acidic protein, biology, business.industry, Neurotoxicity, Brain, Histology, Metabolism, medicine.disease, Pollution, chemistry, Brain Injuries, biology.protein, business, Chickens, Oxidative stress, medicine.drug
Abstract

Dichlorvos (DDVP) is an insecticide with neurotoxicity that is widely used in agricultural production and life. However, the effects of acute DDVP poisoning on brain tissue remain underinvestigated. The purpose of this study was to evaluate the differences within 15 min-6 h in plasma biochemical indexes, brain histology and metabolites among three groups of commercial broilers orally administered different dosages of DDVP one time: (1) high-dose group (11.3 mg/kg), (2) low-dose group (2.48 mg/kg) and (3) control group (0 mg/kg). The results of biochemical indexes showed that acute DDVP poisoning could cause hyperglycemia and oxidative stress in poisoned broilers. Histological examination showed that DDVP could induce brain edema, abnormal expression of glial fibrillary acidic protein (GFAP) and neuronal mitochondrial damage in broilers. Whole-brain metabolism showed that DDVP could significantly change the secretion of neurotransmitters, energy metabolism, amino acid metabolism and nucleotide metabolism. Correlation analysis showed that metabolites such as hypoxanthine, acetylcarnitine and glucose 6-phosphate were significantly correlated with blood glucose, biomarkers of oxidative stress and brain injury pathology. The results of this study provide new insights into the molecular mechanism of brain tissue responses to acute DDVP exposure in broilers and deliver important information for clinical research on neurodegenerative diseases caused by acute DDVP poisoning.

Published
2022

35. The heterotrimeric G protein г Stgg1 is required for conidiation, secondary metabolite production and pathogenicity of Setosphaeria turcica

Author

Pan Li, Xinjie Zhang, Yulan Zhao, Shen Shen, Yibin Lin, Jingao Dong, and Zhimin Hao

Subjects
0301 basic medicine, Setosphaeria turcica, biology, Effector, G protein, pathogenesis, lcsh:Biotechnology, Mutant, Conidiation, biology.organism_classification, Cell biology, 03 medical and health sciences, foliar pathogen, 030104 developmental biology, RNA interference, melanin biosynthesis, lcsh:TP248.13-248.65, Heterotrimeric G protein, HT-toxin, Gene silencing, Biotechnology
Abstract

Heterotrimeric G proteins are best known for their role in the transduction of extracellular signals to various downstream effectors. G proteins in higher eukaryotes are intensively studied; however, their roles in foliar pathogens are still elusive. In this study, we cloned the gene Stgg1 encoding G protein γ subunit in Setosphaeria turcica and investigated its function by RNA interference technology. Three independent Stgg1 targeted RNAi mutants R3, R5 and R6 with diverse silencing efficiency were generated. Knock-down of Stgg1 resulted in a significant reduction in mRNA levels of the genes encoding Gα (Stga1, Stga2, Stga3) but not for Gβ (Stgb1). Stgg1 RNAi mutants exhibited significantly elongated hyphal cells with blocked conidium production. In addition, Stgg1 RNAi mutants all appeared in lighter colony colour compatible with inhibited secondary metabolites. Further assays demonstrated that Stgg1 was required for biosynthesis of melanin and HT-toxin activity. Furthermore, down-regulation of Stgg1 largely inhibited the inflection capacity. Thus, we proposed that Stgg1 played crucial roles in conidiation, secondary metabolite production and pathogenicity of S. turcica and is, therefore, an ideal target for drug design against foliar pathogens.

Published
2018

36. Loss of PDK4 switches the hepatic NF‐κB/TNF pathway from pro‐survival to pro‐apoptosis

Author

Jianguo Wu, Jiajun Zhao, Ji-Young Lee, Ling Gao, Li Wang, Yulan Zhao, and Young-Ki Park

Subjects
Male, 0301 basic medicine, Cell Respiration, PDK4, Apoptosis, Mitochondrion, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Animals, chemistry.chemical_classification, Reactive oxygen species, Hepatology, Tumor Necrosis Factor-alpha, Chemistry, Kinase, NF-kappa B, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, NF-κB, Mitochondria, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, Receptors, Tumor Necrosis Factor, Type I, 030220 oncology & carcinogenesis, Hepatocytes, Tumor necrosis factor alpha, Signal transduction, Signal Transduction
Abstract

It has been established that nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) members promote survival by upregulating antiapoptotic genes and that genetic and pharmacological inhibition of NF-κB is required for tumor necrosis factor (TNF)-induced hepatocyte apoptosis. In this study, we demonstrate that this pro-survival pathway is switched to pro-apoptosis under pyruvate dehydrogenase kinase 4 (PDK4)-deficient conditions. PDK4-deficiency triggered hepatic apoptosis concomitantly with increased numbers of aberrant mitochondria, reactive oxygen species (ROS) production, sustained c-Jun N-terminal Kinase (JNK) activation, and reduction of glutathione (GSH). Interestingly, PDK4 retained p65 in cytoplasm via a direct protein-protein interaction. Disruption of PDK4-p65 association promoted p65 nuclear translocation. This, in turn, facilitated p65 binding to the TNF promoter to activate TNF-TNFR1 apoptotic pathway. Pdk4-/- livers were sensitized to Jo2 and D-(+)-Galactosamine /Lipopolysaccharide (GalN/LPS)-mediated apoptotic injury which was prevented by the inhibition of p65 or TNFR1. The pro-survival activity of TNF was shifted, which was switched to a pro-apoptotic activity in Pdk4-/- hepatocytes as a result of impaired activation of pro-survival NF-κB targets. Conclusion: PDK4 is indispensable to dictate the fate of TNF/NF-κB-mediated hepatocyte apoptosis. (Hepatology 2018).

Published
2018

37. Dynamics and thermal analysis of the auxiliary bearing for the helium circulator of HTR-PM

Author

Zuoyi Zhang, Lei Zhao, Zhengang Shi, Guojun Yang, Xingnan Liu, and Yulan Zhao

Subjects
Materials science, Bearing (mechanical), Rotor (electric), 020209 energy, Circulator, Mechanical engineering, chemistry.chemical_element, Magnetic bearing, 02 engineering and technology, law.invention, 020303 mechanical engineering & transports, 0203 mechanical engineering, Nuclear Energy and Engineering, chemistry, law, 0202 electrical engineering, electronic engineering, information engineering, Lubrication, Bearing capacity, Engineering design process, Helium
Abstract

The high-temperature gas-cooled reactor-pebble bed module (HTR-PM) has been proposed by the Institute of Nuclear and New Energy Technology of Tsinghua University. The helium circulator is equipped in the primary loop of HTR-PM to drive high-temperature helium for energy exchange. The active magnetic bearing (AMB), replaced by the ordinary mechanical bearing, is chosen as the perfect supporting scheme for the helium circulator of HTR-PM. Additionally, the auxiliary bearing is applied in case of AMB failures and has become indispensable to provide mechanical supports and displacement constraints for the rotor. When the rotor drops down, the auxiliary bearing needs to sustain fierce impacts and corresponding thermal growth. While due to the special environment in the primary loop of HTR-PM, oil or grease lubrication can’t be applied in the auxiliary bearing. A new design of unlubricated auxiliary bearing is proposed in this paper. This paper mainly deals with the following two aspects to reveal the rotor drop: dynamic analysis including fatigue life prediction, and corresponding thermal growth. Moreover, dropping experiments are carried out in the HTR-PM helium circulator system. The experimental results can verify the auxiliary bearing capacity to endure the dynamic impact of the dropping rotor, and provide suggestions for the auxiliary bearing type selection. The results in this paper will provide theoretical suggestions for further engineering design and application of the auxiliary bearing in the helium circulator of HTR-PM.

Published
2018

38. Investigating the therapeutic potential and mechanism of curcumin in breast cancer based on RNA sequencing and bioinformatics analysis

Author

Rong Wang, Yulan Zhao, Yapeng Li, Ling Yin, and Jinbin Li

Subjects
0301 basic medicine, Curcumin, Antineoplastic Agents, Breast Neoplasms, MAP3K1, Biology, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Biomarkers, Tumor, Tumor Cells, Cultured, medicine, Humans, Pharmacology (medical), Radiology, Nuclear Medicine and imaging, Protein Interaction Maps, Gene, cDNA library, Gene Expression Profiling, Computational Biology, High-Throughput Nucleotide Sequencing, Cancer, General Medicine, Prognosis, medicine.disease, Molecular biology, Fold change, Gene Expression Regulation, Neoplastic, Gene expression profiling, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer cell, Female
Abstract

Breast cancer is a prevalent cancer in female. This study aims to investigate the therapeutic potential and mechanism of curcumin in breast cancer. After cultivation, human breast cancer cells (MCF-7 cells) were treated with 0.1% (v/v) 15 µmol/ml curcumin-dimethylsulfoxide solution and 0.1% (v/v) dimethylsulfoxide, respectively, at 37 °C and 5% CO2 for 48 h. Total RNA was extracted, cDNA library was constructed, and cDNAs were amplified and sequenced. After data preprocessing, the Cufflinks software was utilized to identify differentially expressed genes (DEGs, |log2 fold change| > 0.5 and p value 0.4). After DEGs screening, 347 DEGs were identified. Up-regulated DEGs were enriched in 14 functions and 3 pathways, and associated with 12 drugs. Down-regulated DEGs were enriched in 14 functions and 9 pathways, and associated with 14 drugs. Moreover, 5 DEGs were associated with breast cancer, including PGAP3, MAP3K1, SERPINE1, PON2, and GSTO2. PPI network was constructed, and the top DEGs were FOS, VIM, FGF2, MAPK1, SPARC, TOMM7, PSMB10, TCEB2, SOCS1, COL4A1, UQCR11, SERPINE1, and ISG15. Curcumin might have therapeutic potential in breast cancer through regulating breast cancer-related genes, including SERPINE1, PGAP3, MAP3K1, MAPK1, GSTO2, VIM, SPARC, and FGF2. However, validations are required.

Published
2017

39. Drop analysis of Active Magnetic Bearing with rolling-sliding integrated auxiliary bearing

Author

Jianqiang Chen, Mingqi Wang, Guojun Yang, Zhengang Shi, Xingnan Liu, and Yulan Zhao

Subjects
History, Materials science, Bearing (mechanical), law, Drop (liquid), Magnetic bearing, Rolling sliding, Composite material, Computer Science Applications, Education, law.invention
Abstract

The Active Magnetic Bearing (AMB) technology is introduced in the High Temperature Reactor-Pebble-bed Modules (HTR-PM) demonstration nuclear power plant, which is being constructed in Shandong province, China. The auxiliary bearing is one of the most important components guaranteeing the reliability of the AMB. It also has an important impact on the reliability of the whole reactor system. Compared with the traditional auxiliary bearing, a novel one proposed by the authors has a smaller impact force and the rotor center orbit is much more concentrated during the rotor drop. This paper establishes an analytical model of drop of rolling-sliding integrated auxiliary bearing to analyze the above phenomena. Based on the Hertz contact theory, the complex structure inside the rolling bearing is simplified through a spring damping model. The overall impact model of the rolling-sliding integrated auxiliary bearing is established. Then, according to the structural characteristics of the rolling-sliding integrated auxiliary bearing, the tangential force inside the rolling- sliding integrated auxiliary bearing can be obtained by applying the angular momentum theorem. Finally, a four-degree-of-freedom horizontal rotor drop model is established to analyze and calculate the center orbit and motion state of the rotor. The analytical model is helpful in the selection and design of auxiliary bearing for AMB. In further research this contact model can be used to calculate the center orbit and contact forces in the application of the rolling-sliding integrated auxiliary bearing.

Published
2021

40. Effects of N-acetyl-l-cysteine on heat stress-induced oxidative stress and inflammation in the hypothalamus of hens

Author

Zheng Xu, Yu Zhuang, Xiaoquan Guo, Guoliang Hu, Lianying Guo, Cong Wu, Lanjiao Xu, Yan Shi, Ruiming Hu, Changming Zhou, Ping Liu, and Yulan Zhao

Subjects
0106 biological sciences, medicine.medical_specialty, NF-E2-Related Factor 2, Physiology, 030310 physiology, Anti-Inflammatory Agents, Hypothalamus, SOD2, Inflammation, Heat Stress Disorders, medicine.disease_cause, 010603 evolutionary biology, 01 natural sciences, Biochemistry, Antioxidants, Proinflammatory cytokine, Avian Proteins, 03 medical and health sciences, Basal (phylogenetics), chemistry.chemical_compound, Internal medicine, medicine, Animals, Poultry Diseases, 0303 health sciences, NF-kappa B, Glutathione, Acetylcysteine, I-kappa B Kinase, PRDX3, Oxidative Stress, Endocrinology, chemistry, Dietary Supplements, Cytokines, Female, medicine.symptom, Oxidoreductases, General Agricultural and Biological Sciences, Chickens, Heat-Shock Response, Oxidative stress, Developmental Biology
Abstract

The purpose of this study was to discuss the effects of N-acetyl-l-cysteine (NAC) on heat stress-induced oxidative stress and inflammation in the hypothalamus of hens in different periods. A total of 120 Hy-Line variety brown laying hens (12 weeks old) were randomly assigned to 4 groups with 6 replicates. The control group (C group) (22 ± 1 °C) received a basal diet, the NAC-treated group (N group) (22 ± 1 °C) received a basal diet with 1000 mg/kg NAC, and 2 heat-stressed groups (36 ± 1 °C for 10 h per day and 22 ± 1 °C for the remaining time) were fed a basal diet (HS group) or a basal diet with 1000 mg/kg NAC (HS + N group) for 21 consecutive days. The influence of NAC on histologic changes, oxidative stress and proinflammatory cytokine production was measured and analysed in hens with heat stress-induced hypothalamic changes. NAC effectively alleviated the hypothalamic morphological changes induced by heat stress. In addition, NAC attenuated the activity of the Nf-κB pathway activated by heat stress and decreased the expression of the proinflammatory cytokines IL-6, IL-18, TNF-α, IKK, and IFN-γ. In addition, NAC treatment regulated the expression of HO-1, GSH, SOD2 and PRDX3 by regulating the activity of Nrf2 at different time points to resist oxidative stress caused by heat exposure. In summary, dietary NAC may be an effective candidate for the treatment and prevention of heat stress-induced hypothalamus injury by preventing Nf-κB activation and controlling the Nrf2 pathway.

Published
2021

41. Metformin rescues Parkin protein expression and mitophagy in high glucose-challenged human renal epithelial cells by inhibiting NF-κB via PP2A activation

Author

Mingjin Sun and Yulan Zhao

Subjects
Ubiquitin-Protein Ligases, Blotting, Western, Mitochondrion, Kidney, Real-Time Polymerase Chain Reaction, General Biochemistry, Genetics and Molecular Biology, Parkin, Mitophagy, Humans, Hypoglycemic Agents, Diabetic Nephropathies, Protein Phosphatase 2, General Pharmacology, Toxicology and Pharmaceutics, Gene knockdown, Chemistry, ATF4, NF-kappa B, Epithelial Cells, General Medicine, Metformin, Cell biology, Mitochondria, Enzyme Activation, IκBα, Glucose, Mitochondrial permeability transition pore, Apoptosis
Abstract

Our preliminary research revealed that metformin, a classic anti-diabetic drug, could rescue Parkin protein expression and mitophagy in high glucose-challenged human renal epithelial cells in vitro, but the molecular mechanism remains to be explored. In the study, Human Renal Cortical Epithelial Cells (HRCEpiC) and Human Renal Proximal Tubular Epithelial Cells (HRPTEpic) were challenged with high glucose with or without metformin pre-treatment to monitor Parkin mRNA and protein expression level change. PRKN gene knockdown was performed by lentiviral-based shRNA delivery. Cell viability, apoptosis and mitophagy were monitored after treatment. Mitochondrial damage was evaluated by analyzing mitochondrial permeability transition pore opening, membrane potential change, mitochondrial superoxide accumulation and cytochrome C release. Protein levels of activating transcription factor 4 (ATF4), p53 phospho-Ser15, IκBα phosphor-Ser32, IKKα phosphor-Ser176/180 in whole cell lysate and nuclear entry of p50/p65 were assessed by western blot. Okadaic acid was used to inhibit protein phosphatase 2A (PP2A). The data suggested high glucose challenge significantly reduced PRKN gene expression, mitophagy, mitochondria integrity and cell viability in vitro, which was rescued by metformin co-treatment. The effects of metformin were crippled by PRKN gene knockdown. Metformin increased PRKN gene transcription while reducednuclear factor kappa B (NF-κB) activation but not that of p53 or ATF4. Inhibiting PP2A weakened NF-κB inhibition and PRKN induction by metformin in high glucose-challenged cells, reducing its mitochondrial protective and cytoprotective effect. So, we concluded thatmetformin protects human renal epithelial cells from high glucose-induced apoptosis by restoring Parkin protein expression and mitophagy via PP2A activation and NF-κB inhibition.

Published
2019

42. A New Algorithm for Identifying Genome Rearrangements in the Mammalian Evolution

Author

Juan Wang, Bo Cui, Yulan Zhao, and Maozu Guo

Subjects
0301 basic medicine, algorithm, lcsh:QH426-470, biology, Phylogenetic tree, genome rearrangements, mammal, Evolution of mammals, Computational biology, biology.organism_classification, Genome, lcsh:Genetics, 03 medical and health sciences, Rhesus macaque, 030104 developmental biology, 0302 clinical medicine, 030220 oncology & carcinogenesis, Simulated data, evolution, Genetics, Molecular Medicine, phylogenetic tree, Genetics (clinical), Original Research
Abstract

Genome rearrangements are the evolutionary events on level of genomes. It is a global view on evolution research of species to analyze the genome rearrangements. We introduce a new method called RGRPT (recovering the genome rearrangements based on phylogenetic tree) used to identify the genome rearrangements. We test the RGRPT using simulated data. The results of experiments show that RGRPT have high sensitivity and specificity compared with other tools when to predict rearrangement events. We use RGRPT to predict the rearrangement events of six mammalian genomes (human, chimpanzee, rhesus macaque, mouse, rat, and dog). RGRPT has recognized a total of 1,157 rearrangement events for them at 10 kb resolution, including 858 reversals, 16 translocations, 249 transpositions, and 34 fusions/fissions. And RGRPT has recognized 475 rearrangement events for them at 50 kb resolution, including 332 reversals, 13 translocations, 94 transpositions, and 36 fusions/fissions. The code source of RGRPT is available from https://github.com/wangjuanimu/data-of-genome-rearrangement.

Published
2019

43. Mobile Health (mHealth) technology for improved screening, patient involvement and optimising integrated care in atrial fibrillation: The mAFA (mAF‐App) II randomised trial

Author

Guo, Yutao, Lane, Deirdre A, Wang, Liming, Chen, Yundai, Lip, Gregory YH, Eckstein, Jens, Thomas, G Neil, Mei, Feng, Xuejun, Liu, Xiaoming, Li, Zhaoliang, Shan, Xiangming, Shi, Wei, Zhang, Yunli, Xing, Jing, Wen, Fan, Wu, Sitong, Yang, Xiaoqing, Jin, Bo, Yang, Xiaojuan, Bai, Yuting, Jiang, Yangxia, Liu, Yingying, Song, Zhongju, Tan, Li, Yang, Tianzhu, Luan, Chunfeng, Niu, Lili, Zhang, Shuyan, Li, Zulu, Wang, Bing, Xv, Liming, Liu, Yuanzhe, Jin, Yunlong, Xia, Xiaohong, Chen, Fang, Wu, Lina, Zhong, Yihong, Sun, Shujie, Jia, Jing, Li, Nan, Li, Shijun, Li, Huixia, Liu, Rong, Li, Fan, Liu, Qingfeng, Ge, Tianyun, Guan, Yuan, Wen, Xin, Li, Yan, Ren, Xiaoping, Chen, Ronghua, Chen, Yun, Shi, Tong, Liu, Yulan, Zhao, Haili, Shi, Yujie, Zhao, Quanchun, Wang, Weidong, Sun, Lin, Wei, Chan, Esther, Guangliang, Shan, Chen, Yao, Wei, Zong, Dandi, Chen, Xiang, Han, Anding, Xu, Xiaohan, Fan, Ziqiang, Yu, Xiang, Gu, Fulin, Ge, and investigators, mAF-App II Trial

Subjects
Adult, Male, Research design, China, medicine.medical_specialty, Adolescent, Cost effectiveness, Cost-Benefit Analysis, 030204 cardiovascular system & hematology, law.invention, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, law, Atrial Fibrillation, Outcome Assessment, Health Care, Health care, Atrial Fibrillation/diagnosis, Humans, Medicine, Prospective Studies, 030212 general & internal medicine, Cluster randomised controlled trial, Patient participation, mHealth, Aged, Delivery of Health Care, Integrated, business.industry, General Medicine, Middle Aged, Telemedicine, Integrated care, Research Design, Emergency medicine, Female, Patient Participation, business
Abstract

BACKGROUND: Current management of patients with atrial fibrillation (AF) is limited by low detection of AF, nonadherence to guidelines and lack of consideration of patient's preferences, thus highlighting the need for a holistic and integrated approach to AF management. The present study aims to determine whether a mHealth technology-supported AF integrated management strategy will reduce AF-related adverse events.METHODS/DESIGN: The mAFA II trial is a prospective, cluster randomized controlled trial. The 40 sites will be randomized to mAFA-integrated care intervention or usual care arms. Prior to randomization, study sites will be paired to be matched in size and the proportion of study eligible patients. All AF patients aged over 18 years old with CHA2 DS2 -VASc score ≥2 will be enrolled. Assuming a composite adverse event rate of 10% pre-intervention, reduced to 5% after intervention, we aim to recruit 3660 patients assuming a 10% loss to follow-up. The primary study endpoint is a composite of stroke/thromboembolism, all-cause death, and rehospitalization. Ancillary analyses would determine patient-related outcome measures, health economics and cost effectiveness, as well as an embedded qualitative study.DISCUSSION: The mAFA II trial will provide evidence for an integrated care approach to holistic AF care, supported by mobile health technology to improve screening, patient involvement and optimization of management. This article is protected by copyright. All rights reserved.

Published
2019

44. Additional file 1: of NFAT5 mediates hypertonic stress-induced atherosclerosis via activating NLRP3 inflammasome in endothelium

Author

Pingping Ma, Shenfang Zha, Xinkun Shen, Yulan Zhao, Li, Li, Yang, Li, Mingxing Lei, and Wanqian Liu

Abstract

Supplementary Materials and Methods. Figure S1. Effect of high salt intake on weight and atherosclerotic lesions formation of ApoE-/- mice. Figure S2. HUVECs adapt well in hypertonic medium. Figure S3 High-salt elevates the expression of vWF in HUVECs. Figure S4. Overexpression of NFAT5 increases Caspase-1 activity in ECs by treated with Ad-null, 5 MOI, Ad-NFAT5, 2.5 MOI or 5 MOI. Figure S5. High Salt-elevated NFAT5 mediate ROS production in ECs via. Figure S6. NFAT5 mediates IL-1β expression. Table S1. The primers sequences for RT-qPCR in this study. Table S2. Effect of high-salt intake on serum level of ApoE-/- mice. (DOCX 1785 kb)

Published
2019
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45. Insomnia and the risk of hypertension: A meta-analysis of prospective cohort studies

Author

Yan He, Yong Gan, Heng Jiang, Chunmei Wu, Qingfeng Tian, Liqing Li, Zuxun Lu, Qiao Liu, Qian Mei, Xiaogang Zhou, and Yulan Zhao

Subjects
Risk, Pulmonary and Respiratory Medicine, medicine.medical_specialty, 03 medical and health sciences, 0302 clinical medicine, Asian People, Risk Factors, Sleep Initiation and Maintenance Disorders, Physiology (medical), Internal medicine, Insomnia, Humans, Medicine, Prospective Studies, Prospective cohort study, Sleep disorder, business.industry, European population, medicine.disease, Confidence interval, 030228 respiratory system, Neurology, Meta-analysis, Relative risk, Hypertension, Cohort, Neurology (clinical), medicine.symptom, business, 030217 neurology & neurosurgery
Abstract

Summary The relationship between insomnia and hypertension remains inconclusive. Thus, we conducted a meta-analysis of prospective cohort studies to evaluate the association between insomnia and the risk of hypertension. Relevant prospective cohort studies were searched from PubMed, Embase, and Web of Science from their inception to October 2019. A random-effects model was used to calculate the pooled relative risk (RR) with 95% confidence interval (CI). A total of fourteen prospective cohort studies involving 395,641 participants were included in this study. The pooled RR of insomnia on hypertension was 1.21 (95%CI: 1.10–1.33). An increased risk of hypertension was observed in participants with difficulty maintaining sleep (RR = 1.27; 95%CI: 1.04–1.55) and early morning awakening (RR = 1.14; 95%CI: 1.08–1.20), but was not statistically significant in participants with difficulty falling asleep (RR = 1.14; 95%CI: 0.95–1.37). In addition, the results were statistically significant in the European population (RR = 1.08, 95%CI: 1.02–1.14), but not significant in Asian and American populations (RR = 1.54, 95%CI: 0.98–2.40; RR = 1.21, 95%CI: 0.89–1.65). The study findings indicate that insomnia is associated with a significantly increased risk of hypertension. This may have substantial implications for the prevention of hypertension in individuals with insomnia symptoms.

Published
2021

46. Research on dynamics and experiments about auxiliary bearings for the helium circulator of the 10MW high temperature gas-cooled reactor

Author

Zhengang Shi, Guojun Yang, Yulan Zhao, Lei Zhao, and Xingnan Liu

Subjects
Angular acceleration, Bearing (mechanical), Materials science, 020209 energy, Drop (liquid), Circulator, Mechanical engineering, chemistry.chemical_element, Magnetic bearing, 02 engineering and technology, Contact force, law.invention, Inet, 020303 mechanical engineering & transports, 0203 mechanical engineering, Nuclear Energy and Engineering, chemistry, law, 0202 electrical engineering, electronic engineering, information engineering, Helium
Abstract

The 10 MW high-temperature gas-cooled reactor (HTR-10) was constructed by the Institute of Nuclear and New Energy Technology (INET) of Tsinghua University. The auxiliary bearing is utilized in this system to meet particular requirements for the reactor. The main role of the auxiliary bearing is to constrain rotor displacements and also to support the rotor when the rotor drops down, which is caused by the active magnetic bearing (AMB) failure. The auxiliary bearing needs to endure huge impact, rapid angular acceleration and thermal shock. On the one hand, complex geometrical constructions and forces applied on the system bring difficulties and restrictions to establish an appropriate model to reveal the actual dynamic process. On the other hand, large volumes of data obtained from experiments show velocities and displacements of the rotor during the rotor drop process and then can indicate the actual dynamic interactions to a great extent. The research in this paper is based on the test rig of the AMB helium circulator of HTR-10. This paper aims to analyze the dynamic performance and contact forces of the rotor by processing experimental data. A measurement to estimate forces developed due to impacts of the rotor and the auxiliary bearings is presented. It is of great significance and provides certain foundation to elaborate the rotor drop process for the AMB helium circulator of HTR-10.

Published
2016

47. Experimental study on the novel rolling-sliding integrated auxiliary bearing in active magnetic bearing during rotor drop

Author

Yan Zhou, Zhengang Shi, Jingjing Zhao, Xunshi Yan, Yulan Zhao, Xingnan Liu, Guojun Yang, and Jianqiang Chen

Subjects
Bearing (mechanical), Materials science, Rotor (electric), 020209 energy, Drop (liquid), Mechanical engineering, Magnetic bearing, 02 engineering and technology, 01 natural sciences, 010305 fluids & plasmas, law.invention, Nuclear Energy and Engineering, law, Position (vector), 0103 physical sciences, 0202 electrical engineering, electronic engineering, information engineering, Orbit (dynamics), Point (geometry), Impact
Abstract

The Active Magnetic Bearing (AMB) is used in the High Temperature Reactor- Pebble-bed Modules (HTR-PM) which is being constructed in Shandong province, China. The auxiliary bearing is one of the most important components guaranteeing the reliability of the AMB. A novel rolling-sliding integrated auxiliary bearing is studied through the drop experiment. For comparison, a traditional rolling auxiliary bearing in the same AMB is also studied experimentally. It was found that the rotor center orbit with the novel auxiliary bearing is much simpler and more focused. The impact force with the novel auxiliary bearing during the drop is much less. The drop process with the novel auxiliary bearing is more stable. All these phenomena are due to the fact that the contact point of the rotor with the novel auxiliary bearing is easy to stay at the lowest position of the circular contact surface during drop process.

Published
2020

48. Functionalized cell-free scaffolds for bone defect repair inspired by self-healing of bone fractures: A review and new perspectives

Author

Lu Hongwei, Qingyi He, Jiangming Luo, Yulan Zhao, Li Li, Wanqian Liu, and Li Yang

Subjects
Materials science, Bone Regeneration, Cell Culture Techniques, Bioengineering, 02 engineering and technology, Bone healing, Cell free, Cell recruitment, 010402 general chemistry, Regenerative Medicine, 01 natural sciences, Regenerative medicine, Bone tissue engineering, Bone and Bones, Biomaterials, Fractures, Bone, Tissue engineering, Osteogenesis, Animals, Humans, Tissue Engineering, Tissue Scaffolds, Cell Differentiation, 021001 nanoscience & nanotechnology, Bone defect, 0104 chemical sciences, Mechanics of Materials, Self-healing, 0210 nano-technology, Biomedical engineering
Abstract

Studies have demonstrated that scaffolds, a component of bone tissue engineering, play an indispensable role in bone repair. However, these scaffolds involving ex-vivo cultivated cells seeded have disadvantages in clinical practice, such as limited autologous cells, time-consuming cell expansion procedures, low survival rate and immune-rejection issues. To overcome these disadvantages, recent focus has been placed on the design of functionalized cell-free scaffolds, instead of cell-seeded scaffolds, that can reduplicate the natural self-healing events of bone fractures, such as inflammation, cell recruitment, vascularization, and osteogenic differentiation. New approaches and applications in tissue engineering and regenerative medicine continue to drive the development of functionalized cell-free scaffolds for bone repair. In this review, the self-healing processes were highlighted, and approaches for the functionalization were summarized. Also, ongoing efforts and breakthroughs in the field of functionalization for bone defect repair were discussed. Finally, a brief summery and new perspectives for functionalization strategies were presented to provide guidelines for further efforts in the design of bioinspired cell-free scaffolds.

Published
2018

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