58 results on '"Yue-Peng Wang"'
Search Results
2. Multimodality Imaging in Diagnosing Left Atrial Appendage Atresia of the Ostium
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Yi Yu, Ming Ding, Yu-Han Chen, Ting Wang, Xiao-Li Tang, Xiao-Hong Huang, Ling-Wei Yu, Yue-Peng Wang, and Yi-Gang Li
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Radiology, Nuclear Medicine and imaging ,Cardiology and Cardiovascular Medicine - Published
- 2023
3. Correction: The association between ADAM12 gene polymorphisms and osteoarthritis: an updated meta-analysis
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Su Yang, Yue-peng Wang, Xi-yong Li, Peng-yong Han, and Peng-fei Han
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Orthopedics and Sports Medicine ,Surgery - Published
- 2023
4. The association between ADAM12 gene polymorphisms and osteoarthritis: an updated meta-analysis
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Su Yang, Yue-peng Wang, Xi-yong Li, Peng-yong Han, and Peng-fei Han
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Orthopedics and Sports Medicine ,Surgery - Abstract
Background Osteoarthritis of the knee is an irreversible disease that causes great pain, and genetic factors play an important role in its occurrence and development. There have been many studies on the correlation between ADAM12 polymorphisms and genetic susceptibility to osteoarthritis, but the results remain inconclusive. Methods Papers from PubMed, Web of Science, EMbase, Springer, SCOPUS, Google Scholar and other databases were systematically retrieved with a cut-off of January 2022. All case–control studies on ADAM12 rs3740199, rs1871054, rs1044122, and rs1278279 polymorphisms and osteoarthritis were searched. Fixed or random effects models were used for pooled analysis with OR values and 95% confidence intervals (CI), and publication bias was assessed. In addition, the false-positive reporting probability test was used to assess the confidence of a statistically significant association. Results Eleven articles were included, which included 3332 patients with osteoarthritis and 5108 healthy controls. Meta-analysis showed that the rs1871054 polymorphism of ADAM12 was associated with osteoarthritis in dominant, recessive, allelic, and homozygote genetic models [C vs. T: OR = 1.34 95% CI (1.05, 1.71), P ADAM12 polymorphism rs1871054 in Asians and osteoarthritis [C vs. T: OR = 1.61, 95% CI (1.25, 2.08), P ADAM12 polymorphism rs1871054 is associated with osteoarthritis in patients younger than 60 years of age [C vs. T: OR = 1.39, 95% CI (1.01, 1.92), P = 0.289]; however, the ADAM12 gene rs3740199, rs1044122, and rs1278279 site polymorphisms were not significantly. Furthermore, when assessing the confidence of the positive results, the positive results were found to be credible (except for Age Conclusion Polymorphism at the rs1871054 site of ADAM12 is associated with genetic susceptibility to osteoarthritis, but rs3740199, rs1044122, and rs1278279 site polymorphisms are not.
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- 2023
5. Screening of diagnostic markers related to immune infiltration in osteoarthritis patients based on machine learning
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Su Yang, Xi-yong Li, Yue-peng Wang, Chang-sheng liao, Peng-yong Han, and Peng-fei Han
- Abstract
Purpose We analyze the immune infiltration model of osteoarthritis to determine the relevant diagnostic biomarkers (OA), and to provide some help for the treatment and diagnosis of OA. Methods From the Gene Expression Omnibus (GEO) database, we downloaded GSE168505 and GSE114007 gene expression datasets, including 24 patients and 21 healthy controls. The R software Limma package and SVA package were used to analyze the batch effect. We selected differentially expressed genes (DEGs), and we then analyzed the DEGs’ functional enrichment. We performed differential analysis to pick out the differentially expressed immune-related genes (DEIRGs) in the merged data set. We first selected the candidate genes by the least absolute shrinkage and selection operator (LASSO) method, and then further screened the diagnostic markers by support vector machine-recursive feature elimination algorithm (SVM-RFE). In dataset GSE129147, the diagnostic value was determined by drawing the receiver operating characteristic (ROC) curve. In addition, we used the CIBERSORT program to assess the 22 kinds immune cells of infiltration models. Finally, an in vitro cell model of OA was established by interleukin-1β(IL-1β) to verify the bioinformatics results. Results Through differential analysis, 454 differential genes were identified, mainly involved ossification, extracellular matrix organization, collagen − containing extracellular matrix, metalloendopeptidase activity, PI3K − Akt signaling pathway, regulation of cell population proliferation, and other biological processes. We screened BIRC5 and TNFSF11 as candidate biomarkers by machine learning. In the data set GSE129147, BIRC5 and TNFSF11 were verified as diagnostic markers of OA by the ROC curve. The following correlation analysis found that BIRC5 and TNFSF11 were correlated with Mast cells resting, NK cells resting, Monocytes, Plasma cells, Eosinophil, Macrophages M0, and Macrophages M2. The expression of BIRC5 and TNFSF11 was up-regulated in the OA model in vitro. Conclusion We conclude that BIRC5 and TNFSF11 can be biomarkers for diagnosing OA. This discovery provides a direction for the occurrence of OA and the exploration of new treatment methods from the perspective of immunology.
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- 2023
6. Diagnostic value of real-time four-dimensional transesophageal echocardiography on the implant-related thrombus
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Yi Yu, Rui Zhang, Yu-Han Chen, Ting Wang, Xiao-Li Tang, Chang-qi Gong, Yun Shao, Zheng Wang, Yue-Peng Wang, and Yi-Gang Li
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Cardiology and Cardiovascular Medicine - Abstract
ObjectivesThis study aims to evaluate the diagnostic value of real-time four-dimensional transesophageal echocardiography (RT4D-TEE) for implant-related thrombus (IRT).MethodsWe collected 1,125 patients with atrial fibrillation from May 2019 to February 2022 in our hospital. All patients accepted transesophageal echocardiography (TEE) examination to exclude any thrombi before the LAAC procedure.ResultsThere were 760 patients with LAAC, 66 patients with CIED, and 299 patients without any implantations. A total of 40 patients with an established diagnosis of IRT were further analyzed. The accurate detection rate of IRT by RT4D-TEE was 4.8% (40/826), which was higher than 3.8% (31/826) by 2D-TEE (P = 0.004). No IRT was found on TEE in the rest of the 786 patients. These 40 patients were divided into LAAC (n = 23) and CIED (n = 17) groups according to the results of RT4D-TEE. In the LAAC group, IRT distributed on different parts of the LAA occluder surface, 91.3% (21/23) with clumps of thrombi, and 8.7% (2/23) with a thin layer of thrombi covering the surface of the occluder. In the CIED group, thrombi were seen attached to the leads in the right atrium and right ventricle. The thrombi were beaded in 17.6% (3/17), corded in 17.6% (3/17), and clotted in the remaining 64.7% (11/17) of cases. After adjusting the anticoagulant dosage and following up for 6 months, 20% (8/40) of cases were successfully resolved, 67.5% (27/40) became smaller, and 12.5% (5/40) showed no changes.ConclusionThe accurate detection rate of IRT by RT4D-TEE was significantly higher than that by 2D-TEE. 2D-TEE has limitations, but RT4D-TEE can be used as an effective complementary method. Imaging and some clinical features differ significantly between IRT on occluder and IRT on CIED lead.
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- 2023
7. Preparation of bulk-like La0.8Sr0.2Ga0.8Mg0.2O3-δ coatings for porous metal-supported solid oxide fuel cells via plasma spraying at increased particle temperatures
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Yue-Peng Wang, Jiu-Tao Gao, Chang-Jiu Li, Li Jiahong, and Cheng-Xin Li
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Equiaxed crystals ,Materials science ,Renewable Energy, Sustainability and the Environment ,Oxide ,Energy Engineering and Power Technology ,chemistry.chemical_element ,Atmospheric-pressure plasma ,Gallate ,engineering.material ,Condensed Matter Physics ,chemistry.chemical_compound ,Fuel Technology ,chemistry ,Coating ,Chemical engineering ,engineering ,Lanthanum ,Particle ,Porosity - Abstract
While porous metal-supported solid fuel oxide cells (PMS-SOFCs) have the potential to decrease the cost and increase the start-up speed of power units, the available fabrication processes remain too cumbersome for industrial production. In this study, we prepared bulk-like strontium and magnesium-doped lanthanum gallate (LSGM) coatings using atmospheric plasma spraying (APS) at an increased particle temperature. The large equiaxed grains inside the coatings indicated the epitaxial growth of the splat interfaces and improvement in the coating quality. With increased particle temperature, the conductivity of dense LSGM coatings directly deposited by APS was comparable to that of the bulk material, and cell performance was also significantly enhanced. The maximum power density of the PMS-SOFC at 700 °C was 831 mW cm−2 and 596 mW cm−2 when high and low particle temperatures were used, respectively. These results indicated that the quality of the coating was improved by increasing the in-flight particle temperature.
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- 2021
8. Oxidation behavior and interface diffusion of porous metal supported SOFCs with all plasma sprayed functional layers in air at 650oC
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Chang-Jiu Li, Cheng-Xin Li, Yue-Peng Wang, and Jiu-Tao Gao
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Porous metal ,Materials science ,Renewable Energy, Sustainability and the Environment ,Interface (Java) ,Diffusion ,Oxide ,Metal ,chemistry.chemical_compound ,Electricity generation ,chemistry ,Chemical engineering ,Plasma sprayed ,visual_art ,visual_art.visual_art_medium ,Fuel cells - Abstract
metal supported solid oxide fuel cells (PMS-SOFCs) are regarded as a promising choice for power generation. 430 L steel is a popular choice for SOFC support due to its acceptable electrical perform...
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- 2021
9. Multimodality Imaging Assessment of Intramyocardial Hematoma
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Yi Yu, Ming Ding, Ting Wang, Xiao-Li Tang, Xiao-Hong Huang, Ling-Wei Yu, Yue-Peng Wang, and Yi-Gang Li
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Hematoma ,Humans ,Radiology, Nuclear Medicine and imaging ,Cardiology and Cardiovascular Medicine ,Multimodal Imaging - Published
- 2022
10. Vascular Adventitial Fibroblasts-Derived FGF10 Promotes Vascular Smooth Muscle Cells Proliferation and Migration in vitro and the Neointima Formation in vivo
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Zhenwei Yang, Zhiyong Chen, Xueze Jiang, Jie Chen, Xuesheng Hua, Mengkun Shi, Yue-Peng Wang, Yuanyuan Chen, and Yuhan Chen
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0301 basic medicine ,MAPK/ERK pathway ,Neointima ,Vascular smooth muscle ,proliferation ,Immunology ,migration ,Fibroblast growth factor ,03 medical and health sciences ,0302 clinical medicine ,vascular smooth muscle cells ,Immunology and Allergy ,Vascular tissue ,Original Research ,FGF10 ,biology ,Chemistry ,musculoskeletal system ,Cell biology ,stomatognathic diseases ,030104 developmental biology ,030220 oncology & carcinogenesis ,cardiovascular system ,biology.protein ,neointima formation ,Signal transduction ,Journal of Inflammation Research ,tissues ,Platelet-derived growth factor receptor ,vascular adventitial fibroblasts - Abstract
Yuhan Chen,1,* Yuanyuan Chen,1,* Xueze Jiang,1 Mengkun Shi,2 Zhenwei Yang,1 Zhiyong Chen,1 Xuesheng Hua,1 Jie Chen,1 Yuepeng Wang1 1Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200092, People’s Republic of China; 2Department of Cardio-Thoracic Surgery, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuepeng Wang; Jie ChenDepartment of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200092, People’s Republic of ChinaTel +86 18217267289; +86 15280380968Email wangyuepeng@xinhuamed.com.cn; jieyuepeng@msn.comBackground: Activation of vascular adventitial fibroblasts (VAFs) upon vascular injury contributes greatly to the medial vascular smooth muscle cells (VSMCs) proliferation, migration and the subsequent neointima formation. A number of factors including fibroblast growth factors (FGFs) have been shown to control VSMC growth, proliferation and phenotypic switching, suggesting that they may function as paracrine signals for VAFs to modulate VSMCs functions. However, little is known about the signaling molecule(s) and its mechanism of action. This study is set to identify which and how FGF family members are involved in VAFs mediated vascular remodeling.Methods: We used qPCR, Western blot and Immunohistochemistry to observe the spatiotemporal expression of FGF10 and FGFR2 in injured vascular tissue. The proliferation and migration assays of VSMCs were performed in a co-culture system. The activation of signaling pathway was detected by Western blot, immunohistochemistry and immunofluorescence. Hematoxylin-eosin and immunofluorescence were used to assess the effects of exogenous FGF10 and siFGF10 on the neointima formation.Results: The expression of FGF10 and FGFR2 were increased from day 3 through day 14 post injury. FGF10 was significantly upregulated in adventitia, and FGFR2 was detected in both media and neointima after injury. In vitro, FGF10 was most prominently expressed in VAFs and FGFR2 was significantly expressed in VSMCs. Both were regulated by PDGF. Co-culture of VAFs and VSMCs in vitro showed that VAF-derived FGF10 promoted the proliferation and migration of VSMCs. PDGF could synergistically enhance the process. VAF-derived FGF10 can significantly activate the FGFR2 in VSMCs and furthermore significantly activate the downstream MAPK/PI3K-AKT signaling pathways. Delivery of exogenous FGF10 potentiated the neointima formation, while siFGF10 attenuated the neointima formation.Conclusion: VAFs-derived FGF10 promoted the proliferation and migration of VSMCs and neointima formation, and FGF10-FGFR2 signaling triggered the activation of MAPK/PI3K-AKT pathways in VSMCs and PDGF synergistically amplified FGF10 signaling.Keywords: vascular adventitial fibroblasts, vascular smooth muscle cells, FGF10, proliferation, migration, neointima formation
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- 2021
11. Microstructural analysis of highly active cathode material La0.7Sr0.3Ti0.15Fe0.65Ni0.2O3-δ (LSTFN) by optimizing different processing parameters
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Jiu-Tao Gao, Cheng-Xin Li, Muhammad Bilal Hanif, Chang-Jiu Li, Kausar Shaheen, Muhammad Yasir, Yue-Peng Wang, and Sana qayyum
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010302 applied physics ,Materials science ,Fabrication ,Scanning electron microscope ,Process Chemistry and Technology ,Sintering ,02 engineering and technology ,Electrolyte ,engineering.material ,021001 nanoscience & nanotechnology ,01 natural sciences ,Cathode ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Dielectric spectroscopy ,law.invention ,Coating ,law ,0103 physical sciences ,Materials Chemistry ,Ceramics and Composites ,engineering ,Composite material ,0210 nano-technology ,Polarization (electrochemistry) - Abstract
The modified Pechini method was applied to prepare a highly active and novel cathode material La0.7Sr0.3Ti0.15Fe0.65Ni0.2O3-δ (LSTFN). This material was coated on the LGSM electrolyte through a screen-printing technique with variable thicknesses of 28 ± 8, 41 ± 8, and 62 ± 8 μm, respectively. Different fabrication parameters, including sintering temperature, time, coating thickness, and variations in ball-milling, which affect the electrochemical performance of the cathode material, were investigated. X-ray diffraction analysis of the cathode material suggested that it exhibits a cubic crystal structure with a LSTFN single phase. The morphological studies were conducted using scanning electron microscopy (SEM), which confirmed that the electrode material had a highly porous structure. Meanwhile, the electrochemical properties of the material were studied by electrochemical impedance spectroscopy (EIS), which revealed that by varying different parameters, the electrochemical performance of the electrode material was enhanced. The coated cathode materials with variable thicknesses were analyzed at different sintering temperatures and times. Experimental results suggest that the optimum sintering temperature and time were 950 °C and 3 h, respectively, at which LSTFN exhibits the minimum polarization resistance (RP) of 0.046 Ωcm2 when sintered at 800 °C for 3 h.
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- 2021
12. Performance and Stability of Plasma-Sprayed 10 × 10 cm2 Self-sealing Metal-Supported Solid Oxide Fuel Cells
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Li Jiahong, Cheng-Xin Li, Yue-Peng Wang, Jiu-Tao Gao, and Chang-Jiu Li
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010302 applied physics ,Materials science ,Open-circuit voltage ,Oxide ,02 engineering and technology ,Interconnector ,Temperature cycling ,Condensed Matter Physics ,01 natural sciences ,Thermal expansion ,Surfaces, Coatings and Films ,chemistry.chemical_compound ,020303 mechanical engineering & transports ,0203 mechanical engineering ,chemistry ,Soldering ,0103 physical sciences ,Materials Chemistry ,Brazing ,Solid oxide fuel cell ,Composite material - Abstract
This study adopts a novel structure design of large-area planar metal-supported solid oxide fuel cell (MS-SOFC), which exhibits the characteristics of self-sealing and high thermal cycling resistance. The self-sealing structure of MS-SOFCs is achieved by brazing technology. Plasma spraying is performed to prepare all functional layers of cells. The size of cells is 10 × 10 cm2. The coefficients of thermal expansion of relevant parts (i.e., porous metal support, interconnector and compound structure of both) and the electrical conductivity of brazing solder were measured. The microstructure of brazing area was observed in situ at 800 °C for 500 h, and the distribution of fuel gas in the interconnector was simulated. The performance of cells was characterized and found that the maximum power density can reach up to 716 mW cm−2 at 700 °C. Moreover, the long-term stability of the cell was investigated for about 500 h with 6 times of thermal cycling implemented during this period. The open circuit voltage and the maximum power density of the cell showed a good stability.
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- 2021
13. Cadherin‐11 deficiency mitigates high‐fat diet‐induced inflammatory atrial remodeling and vulnerability to atrial fibrillation
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Yi Wan, Long Chen, Lei Zhou, Yingze Li, Shuai Song, Jia-Li Yuan, Guojian Fang, Ying Xiao, Zhuowang Ge, Wei Cao, Qun-Shan Wang, Yudong Fei, Yue-Peng Wang, and Yuhan Chen
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0301 basic medicine ,medicine.medical_specialty ,Atrial enlargement ,Physiology ,Clinical Biochemistry ,Inflammation ,Diet, High-Fat ,Proinflammatory cytokine ,Impaired glucose tolerance ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Internal medicine ,Atrial Fibrillation ,Hyperlipidemia ,medicine ,Animals ,Humans ,Heart Atria ,cardiovascular diseases ,Cadherin ,business.industry ,Atrial fibrillation ,Atrial Remodeling ,Cell Biology ,Cadherins ,medicine.disease ,030104 developmental biology ,Endocrinology ,030220 oncology & carcinogenesis ,cardiovascular system ,medicine.symptom ,Cardiomyopathies ,business - Abstract
Atrial fibrillation (AF) is the most common cardiac arrhythmia nowadays. The occurrence of AF is closely associated with obesity. Cadherin-11 (Cad-11), as a member of the cadherin family, can make a contribution to diet-induced obesity and it will be informative to know whether Cad-11 exerts its effects on atrial remodeling and AF vulnerability in a diet-induced obesity model. In this study, we demonstrated that the expression of Cad-11 was significantly upregulated in the left atrium of AF patients with obesity and mice following 16 weeks of high-fat diet (HFD) feeding. Further confirmed that Cad-11 could regulate the activity of atrial fibroblasts by participating in inducing proinflammatory cytokines production. At animal levels, we found that although there was a lack of statistical difference in body weight, Cad-11-/- mice could markedly improve impaired glucose tolerance and hyperlipidemia. Adverse atrial structural remodeling, including atrial enlargement, inflammation, and fibrosis provoked by HFD feeding were mitigated in Cad-11-/- mice. Mechanistically, Cad-11 activated mitogen-activated protein kinases and nuclear factor-κB for interleukin-6 production in atrial fibroblasts that may contribute to the atrial fibrosis process in obesity-related AF, suggesting Cad-11 might be a new therapeutic target for obesity-related AF.
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- 2021
14. Reverse shoulder arthroplasty vs. hemiarthroplasty for the treatment of osteoporotic proximal humeral fractures in elderly patients: A systematic review and meta‑analysis update
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Peng-Fei Han, Su Yang, Yue-Peng Wang, Xue-Dong Hou, Yuan Li, and Xi-Yong Li
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Cancer Research ,Immunology and Microbiology (miscellaneous) ,General Medicine - Abstract
The present meta-analysis was conducted to compare the safety and effectiveness of reverse shoulder arthroplasty (RSA) and hemiarthroplasty (HA) in the treatment of osteoporotic proximal humeral fractures in elderly patients. The Embase, Pubmed Central, Cumulative Index to Nursing and Allied Health Literature, ProQuest Dissertations and Theses, Cochrane Library and Chinese Biomedical databases were searched between January 2009 and January 2022 to identify relevant studies. According to the search strategy, a total of 210 associated studies were retrieved and 16 were finally included. Review Manager 5.4 software was used for the data analysis. This study indicated that patients in the RSA group had significantly improved treatment outcomes compared with patients in the HA group, as assessed by Constant-Murley Shoulder Outcome Score (95% CI, 1.69-3.76; P0.001), American Shoulder and Elbow Surgeons score (95% CI, 11.81-24.88; P0.001) and shoulder range of motion (ROM; 95% CI, 3.41-9.07; P0.001). However, the HA group was superior to the RSA group in terms of the Oxford Shoulder score (95% CI, 2.89-11.11; P0.001). There was no significant statistical difference between the two groups in terms of the Disabilities of the Arm, Shoulder and Hand score and complications. Overall, for the treatment of osteoporotic proximal humeral fractures in the elderly, the RSA group had improved postoperative ROM and functional scores compared with the HA group, without significant difference in the incidence of complications. However, HA remains a safe and reliable treatment option.
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- 2022
15. Self-Sealing Metal-Supported SOFC Fabricated by Plasma Spraying and Its Performance under Unbalanced Gas Pressure
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Chang-Jiu Li, Yue-Peng Wang, Li Jiahong, Jiu-Tao Gao, and Cheng-Xin Li
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010302 applied physics ,Materials science ,02 engineering and technology ,Interconnector ,Electrolyte ,Condensed Matter Physics ,01 natural sciences ,Cathode ,Surfaces, Coatings and Films ,Anode ,law.invention ,Cathodic protection ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Fuel gas ,law ,0103 physical sciences ,Materials Chemistry ,Brazing ,Composite material ,Power density - Abstract
A novel self-sealing structure for metal-supported solid oxide fuel cells (MS-SOFCs) is designed by applying brazing technology between the metal support and interconnector to solve the sealing problem on the anode side of planar SOFCs. A high-reliability self-sealing effect is thus realized at the anode side of the MS-SOFC. Plasma spraying technology is used to prepare cell functional layers including the anode, cathode, and electrolyte. A single cell is assembled with a 50–60-μm plasma-sprayed Sc2O3-stabilized ZrO2 (ScSZ) electrolyte layer. The gas permeability of the self-sealed MS-SOFC without a cathode layer is 0.42 × 10−17 m2. The open-circuit voltage of the cell is ~ 1.1 V in the operating temperature range from 550 to 750 °C. The power density of the cell reaches 1109 mW cm−2 at 750 °C under standard atmosphere. In addition, the use of a fuel gas pressure that is 20 kPa higher than the cathodic gas pressure results in a significant increase in the power density to 1782 mW cm−2 at 750 °C. The novel cell structure and gas tightness of the ScSZ electrolyte prepared by plasma spraying indicates that it can meet the requirements of SOFC applications.
- Published
- 2020
16. Study on Deposition Behavior of Less Than 5 μm YSZ Particles in VLPPS
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Yue-Peng Wang, Jiu-Tao Gao, Lu-Xun Cheng, Chang-Jiu Li, and Cheng-Xin Li
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010302 applied physics ,Materials science ,Plasma jet ,02 engineering and technology ,Substrate (electronics) ,Plasma ,engineering.material ,Condensed Matter Physics ,01 natural sciences ,Flattening ,Surfaces, Coatings and Films ,020303 mechanical engineering & transports ,Deposition (aerosol physics) ,0203 mechanical engineering ,Coating ,Permeability (electromagnetism) ,0103 physical sciences ,Materials Chemistry ,engineering ,Composite material ,Yttria-stabilized zirconia - Abstract
A particular YSZ feedstock for very low-pressure plasma spraying (VLPPS) has been designed which can be automatically divided into less than 5 μm YSZ molten particles in the long plasma jet. Fully molten particles were deposited on the substrate at different deposition distances of 250, 350 and 450 mm, respectively. The deposition behavior of less than 5 μm YSZ molten particles were studied aiming to obtain a thin gastight YSZ coating. The flattening ratio of particles at different deposition distances and the gas permeability of YSZ coating prepared by VLPPS were investigated. The results revealed that the thickness of flattened particles was about 0.10-0.35 μm and the flattening ratio of molten particles was about 4.7. The flattened particles were bonded well with substrates and the width of vertical cracks appeared in flattened particles was 0.01-0.02 μm. The gas permeability of coatings prepared at 350 mm was 1.5 × 10−7 cm4 (gf)−1 s−1.
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- 2020
17. Development of ScSZ Electrolyte by Very Low Pressure Plasma Spraying for High-Performance Metal-Supported SOFCs
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Guan-Jun Yang, Jiu-Tao Gao, Wei Chen, Cheng-Xin Li, Yue-Peng Wang, Shan-Lin Zhang, and Chang-Jiu Li
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010302 applied physics ,Materials science ,Oxide ,02 engineering and technology ,Electrolyte ,engineering.material ,Condensed Matter Physics ,Microstructure ,01 natural sciences ,Surfaces, Coatings and Films ,chemistry.chemical_compound ,020303 mechanical engineering & transports ,0203 mechanical engineering ,chemistry ,Coating ,0103 physical sciences ,Materials Chemistry ,engineering ,Lamellar structure ,Cubic zirconia ,Composite material ,Porosity ,Power density - Abstract
Very low pressure plasma spraying (VLPPS) is an attractive method for metal-supported solid oxide fuel cells, wherein it can significantly avoid the oxidation of metal substrate. In this study, scandia-stabilized zirconia electrolyte was fabricated by VLPPS. To investigate the microstructure of coatings, the spraying distances were set to 150, 250 and 350 mm. The fractured morphology suggests that, at a relatively low deposition temperature (
- Published
- 2019
18. EZH2 as a novel therapeutic target for atrial fibrosis and atrial fibrillation
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Yue-Peng Wang, Liang Liu, Ying Yu, Bin-Feng Mo, Yi-Gang Li, Long Chen, Yi Wan, Rui Zhang, Yi Yu, Qun-Shan Wang, Guojian Fang, Wei Cao, Shuai Song, and Gu Yue
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Male ,0301 basic medicine ,Indoles ,Atrial enlargement ,Pyridones ,macromolecular substances ,SMAD ,030204 cardiovascular system & hematology ,Mice ,03 medical and health sciences ,Dogs ,0302 clinical medicine ,Fibrosis ,Atrial Fibrillation ,medicine ,Animals ,Humans ,Gene silencing ,Enhancer of Zeste Homolog 2 Protein ,Heart Atria ,cardiovascular diseases ,Fibroblast ,Molecular Biology ,business.industry ,Angiotensin II ,EZH2 ,Atrial fibrillation ,Fibroblasts ,Middle Aged ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,cardiovascular system ,Cancer research ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Abstract
Angiotensin II (Ang-II)-induced fibroblast differentiation plays an important role in the development of atrial fibrosis and atrial fibrillation (AF). Here, we show that the expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2) is increased in atrial muscle and atrial fibroblasts in patients with AF, accompanied by significant atrial fibrosis and atrial fibroblast differentiation. In addition, EZH2 is induced in murine models of atrial fibrosis. Furthermore, either pharmacological GSK126 inhibition or molecular silencing of EZH2 can inhibit the differentiation of atrial fibroblasts and the ability to produce ECM induced by Ang-II. Simultaneously, inhibition of EZH2 can block the Ang-II-induced migration of atrial fibroblasts. We found that EZH2 promotes fibroblast differentiation mainly through the Smad signaling pathway and can form a transcription complex with Smad2 to bind to the promoter region of the ACTA2 gene. Finally, our in vivo experiments demonstrated that the EZH2 inhibitor GSK126 significantly inhibited Ang-II-induced atrial enlargement and fibrosis and reduced AF vulnerability. Our results demonstrate that targeting EZH2 or EZH2-regulated genes might present therapeutic potential in AF.
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- 2019
19. Characterization of Self-Sealed Metal Supported SOFCs with the Very Low Pressure Plasma Sprayed ScSZ Electrolyte
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Li Chengxin, Li Changjiu, Shan-Lin Zhang, Yue-Peng Wang, Jiu-Tao Gao, Si Yuan Kang, and Yang Guanjun
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Low pressure plasma ,Metal ,Materials science ,Chemical engineering ,visual_art ,visual_art.visual_art_medium ,Electrolyte ,Characterization (materials science) - Published
- 2019
20. Microstructural evolution of alumina coatings by a novel long laminar plasma spraying method
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Yue-Peng Wang, Cheng-Xin Li, Shan-Lin Zhang, Sen-Hui Liu, Lu Li, Hui-Yu Zhang, Gang Ji, Jia-Hua Huang, Pan Xu, and Chang-Jiu Li
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010302 applied physics ,Argon ,Materials science ,chemistry.chemical_element ,Atmospheric-pressure plasma ,Laminar flow ,02 engineering and technology ,Surfaces and Interfaces ,General Chemistry ,Plasma ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Microstructure ,01 natural sciences ,Surfaces, Coatings and Films ,Volumetric flow rate ,Volume (thermodynamics) ,chemistry ,0103 physical sciences ,Materials Chemistry ,Lamellar structure ,Composite material ,0210 nano-technology - Abstract
A super long and stable laminar plasma jet was used in the atmospheric plasma spray process in this work. Microstructures evolution and properties of alumina coatings that were obtained using a long spraying distance ranging from 200 mm to 350 mm in an atmospheric environment were studied in this work. These processes were carried out by using a total gas flow rate of 14 slpm with 70% nitrogen and 30% argon in volume and output power of 25.4 kW (current of 160 A). A long particle residence time was obtained in this study, which compared with other current atmospheric plasma spray methods. The microstructures of coatings showed the multi-island protrusions on the top surfaces and overlapped lamellar splats at the fracture surfaces. The microstructural evolution was significantly affected by the heating and motion behaviors of alumina particles when these were flowing in the long laminar plasma jet at different spraying distances.
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- 2019
21. Influence of Surface Effect on the Equivalent Properties of Nanoporous Materials with Elastic Waves
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Yue-peng Wang, Yi-feng Hu, and Yan Ru
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Surface (mathematics) ,Physics::Biological Physics ,Quantitative Biology::Biomolecules ,Materials science ,Nanoporous ,Scattering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Moduli ,Quantitative Biology::Subcellular Processes ,Shear (sheet metal) ,Stress (mechanics) ,Matrix (mathematics) ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Composite material ,0210 nano-technology ,Displacement (fluid) - Abstract
The multiple scattering of elastic waves by uniformly arranged nanopores and the equivalent properties of nanoporous materials are studied in this paper. By using displacement potential function method, the stress fileds and strain fields around nanopores and the matrix are solved in accordance with the classical elasticity theory and surface elasticity theory. The equivalent bulk moduli and shear moduli of nanoporous materials are studied using the Mori-Tanaka method. Results show that the equivalent properties of nanoporous materials not only depend on the surface parameters but are also affected by the volume percentage of the holes.
- Published
- 2021
22. The transient receptor potential melastatin 4 channel inhibitor 9-phenanthrol modulates cardiac sodium channel
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Yi-Gang Li, Wei Li, Jian-Wen Hou, Qian Wang, Yue-Peng Wang, Yi-He Chen, Yudong Fei, and Ying Xiao
- Subjects
0301 basic medicine ,Pharmacology ,Chemistry ,Sodium channel ,HEK 293 cells ,Gating ,030204 cardiovascular system & hematology ,03 medical and health sciences ,Transient receptor potential channel ,Dose–response relationship ,030104 developmental biology ,0302 clinical medicine ,Myocyte ,Patch clamp ,IC50 - Abstract
Background and purpose 9-Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9-phenanthrol. Experimental approach Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild-type and mutated human recombinant SCN5A were expressed in HEK293 cells. Anemonia toxin II (ATX-II) was used to amplify the late sodium current (INaL ) and induce arrhythmias. Whole-cell patch clamp technique was used to record APs and ionic currents. Key results 9-Phenanthrol (10-50 μM) stabilized ventricular repolarization and abolished arrhythmias induced by ATX-II in both isolated VMs, PCs and wedge preparations. Further study revealed that 9-phenanthrol modulated the gating properties of cardiac sodium channels and dose-dependently inhibited INaL and peak sodium current (INaP ) in VMs with an IC50 of 18 and 71.5 μM respectively. Its ability to inhibit INaL was further confirmed in PCs and HEK293 cells expressing SCN5A mutations. Conclusions and implications Our results indicate that 9-phenanthrol modulates the gating properties of cardiac sodium channels and inhibits INaL and INaP , which may contribute to its antiarrhythmic and cardioprotective effects.
- Published
- 2018
23. ICaL and Ito mediate rate-dependent repolarization in rabbit atrial myocytes
- Author
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Yi-Gang Li, Yudong Fei, Wei Li, Jian-Wen Hou, Yi-He Chen, Qian Wang, and Yue-Peng Wang
- Subjects
medicine.medical_specialty ,Physiology ,Chemistry ,Sodium channel ,4-Aminopyridine ,Atrial fibrillation ,General Medicine ,030204 cardiovascular system & hematology ,medicine.disease ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,Nifedipine ,Internal medicine ,medicine ,Repolarization ,Atrial myocytes ,Atrium (heart) ,030217 neurology & neurosurgery ,medicine.drug ,Anti-Arrhythmia Agents - Abstract
Rate-dependent repolarization (RDR) of action potential (AP) in cardiomyocyte plays a critical role in the genesis of arrhythmias and RDR in atrium has been linked with atrial fibrillation. However, detailed studies focusing on the role of RDR in rabbit atrium are scant. In this study, atrial cells were isolated from rabbit heart and rate-dependent property was explored in single atrial cell to elucidate the underlying mechanism. Our results indicated that rate-dependent prolongation was evident at the action potential duration at 20% (APD20) and 50% (APD50) repolarization but not at 90% repolarization (APD90) under control condition. Using transient outward potassium current (Ito) inhibitor 4-Aminopyridine (4-AP, 2 mM) effectively eliminated the changes in APD20 and APD50, and unmasked the rate-dependent reduction of APD90 which could be diminished by further adding L-type calcium current (ICaL) inhibitor nifedipine (30 μM). However, using the selective late sodium current (INaL) inhibitor GS-458967 (GS967, 1 μM) caused minimal effect on APD90 of atrial cells both in the absence and presence of 4-AP. In consistence with results from APs, Ito and ICaL displayed significant rate-dependent reduction because of their slow reactivation kinetics. In addition, the magnitude of INaL in rabbit atrium was so small that its rate-dependent changes were negligible. In conclusion, our study demonstrated that Ito and ICaL mediate RDR of AP in rabbit atrium, while minimal effect of INaL was seen.
- Published
- 2017
24. Crucial Role of ROCK2-Mediated Phosphorylation and Upregulation of FHOD3 in the Pathogenesis of Angiotensin II–Induced Cardiac Hypertrophy
- Author
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Wei-Ping Xu, Qing Zhou, Jian-Wen Hou, Hong Wang, Yi-Gang Li, Jie Chen, Si-Si Wei, Gang Li, Xiao-Meng Chen, Qian Wang, Yue-Peng Wang, and Wei Li
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,RHOA ,Pyridines ,Blotting, Western ,Formins ,Cardiomegaly ,Real-Time Polymerase Chain Reaction ,Rats, Sprague-Dawley ,Random Allocation ,03 medical and health sciences ,Downregulation and upregulation ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Myocytes, Cardiac ,ROCK1 ,Phosphorylation ,Protein kinase A ,Rho-associated protein kinase ,Cells, Cultured ,Analysis of Variance ,rho-Associated Kinases ,biology ,Kinase ,Angiotensin II ,Microfilament Proteins ,Amides ,Rats ,Up-Regulation ,Cell biology ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,biology.protein ,Signal Transduction - Abstract
Cardiac hypertrophy is characterized by increased myofibrillogenesis. Angiotensin II (Ang-II) is an essential mediator of the pressure overload–induced cardiac hypertrophy in part through RhoA/ROCK (small GTPase/Rho-associated coiled-coil containing protein kinase) pathway. FHOD3 (formin homology 2 domain containing 3), a cardiac-restricted member of diaphanous-related formins, is crucial in regulating myofibrillogenesis in cardiomyocytes. FHOD3 maintains inactive through autoinhibition by an intramolecular interaction between its C- and N-terminal domains. Phosphorylation of the 3 highly conserved residues (1406S, 1412S, and 1416T) within the C terminus (CT) of FHOD3 by ROCK1 is sufficient for its activation. However, it is unclear whether ROCK-mediated FHOD3 activation plays a role in the pathogenesis of Ang-II–induced cardiac hypertrophy. In this study, we detected increases in FHOD3 expression and phosphorylation in cardiomyocytes from Ang-II–induced rat cardiac hypertrophy models. Valsartan attenuated such increases. In cultured neonate rat cardiomyocytes, overexpression of phosphor-mimetic mutant FHOD3-DDD, but not wild-type FHOD3, resulted in myofibrillogenesis and cardiomyocyte hypertrophy. Expression of a phosphor-resistant mutant FHOD3-AAA completely abolished myofibrillogenesis and attenuated Ang-II–induced cardiomyocyte hypertrophy. Pretreatment of neonate rat cardiomyocytes with ROCK inhibitor Y27632 reduced Ang-II–induced FHOD3 activation and upregulation, suggesting the involvement of ROCK activities. Silencing of ROCK2, but not ROCK1, in neonate rat cardiomyocytes, significantly lessened Ang-II–induced cardiomyocyte hypertrophy. ROCK2 can directly phosphorylate FHOD3 at both 1412S and 1416T in vitro and is more potent than ROCK1. Both kinases failed to phosphorylate 1406S. Coexpression of FHOD3 with constitutively active ROCK2 induced more stress fiber formation than that with constitutively active ROCK1. Collectively, our results demonstrated the importance of ROCK2 regulated FHOD3 expression and activation in Ang-II–induced myofibrillogenesis, thus provided a novel mechanism for the pathogenesis of Ang-II–induced cardiac hypertrophy.
- Published
- 2017
25. Haplodeficiency of activin receptor-like kinase 4 alleviates myocardial infarction-induced cardiac fibrosis and preserves cardiac function
- Author
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Qing Zhou, Chang-Yi Li, Jie Chen, Xiao-Meng Chen, Qian Wang, Yue-Peng Wang, Yi-Gang Li, Yi-He Chen, and Jian-Wen Hou
- Subjects
0301 basic medicine ,Cardiac function curve ,MAPK/ERK pathway ,medicine.medical_specialty ,Genotype ,Cardiac fibrosis ,Myocardial Infarction ,Haploinsufficiency ,Mice ,03 medical and health sciences ,Downregulation and upregulation ,Cell Movement ,Fibrosis ,Internal medicine ,Animals ,Humans ,Ventricular Function ,Medicine ,Smad3 Protein ,Myocardial infarction ,Mortality ,Myofibroblasts ,Molecular Biology ,Cell Proliferation ,Smad4 Protein ,Mice, Knockout ,business.industry ,Hypoxia (medical) ,medicine.disease ,Immunohistochemistry ,Extracellular Matrix ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,Gene Expression Regulation ,Echocardiography ,Heart failure ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Activin Receptors, Type I ,Signal Transduction - Abstract
Cardiac fibrosis (CF), a repairing process following myocardial infarction (MI), is characterized by abnormal proliferation of cardiac fibroblasts and excessive deposition of extracellular matrix (ECM) resulting in inevitable resultant heart failure. TGF-β (transforming growth factor-β)/ALK5 (Activin receptor-like kinase 5)/Smad2/3/4 pathways have been reported to be involved in the process. Recent studies have implicated both activin and its specific downstream component ALK4 in stimulating fibrosis in non-cardiac organs. We recently reported that ALK4 is upregulated in the pressure-overloaded heart and its partial inhibition attenuated the pressure overload-induced CF and cardiac dysfunction. However, the role of ALK4 in the pathogenesis of MI-induced CF, which is usually more severe than that induced by pressure-overload, remains unknown. Here we report: 1) In a wild-type mouse model of MI, ALK4 upregulation was restricted in the fibroblasts of the infarct border zone; 2) In contrast, ALK4+/- mice with a haplodeficiency of ALK4 gene, showed a significantly attenuated CF in the border zone, with a smaller scar size, a preserved cardiac function and an improved survival rate post-MI; 3) Similarly to pressure-overloaded heart, these beneficial effects might be through a partial inactivation of the Smad3/4 pathway but not MAPK cascades; 4) The apoptotic rate of the cardiomyocytes were indistinguishable in the border zone of the wild-type control and ALK4+/- mice; 5) Cardiac fibroblasts isolated from ALK4+/- mice showed reduced migration, proliferation and ECM synthesis in response to hypoxia. These results indicate that partial inhibition of ALK4 may reduce MI-induced CF, suggesting ALK4 as a novel target for inhibition of unfavorable CF and for preservation of LV systolic function induced by not only pressure-overload but also MI.
- Published
- 2017
26. Development of ScSZ Electrolyte Fabricated by Very Low Pressure Plasma Spraying for Metal Supported SOFCs Applications
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Yue-Peng Wang, Jiu-Tao Gao, Wei Chen, Cheng-Xin Li, Shan-lin Zhang, and Guan-Jun Yang
- Abstract
This study assesses the potential of scandia-stabilized zirconia (ScSZ) produced by very low-pressure plasma spraying (VLPPS) for metal-supported solid oxide fuel cell (MS-SOFC) applications. To investigate the microstructure of ScSZ, coating samples were deposited at spraying distances of 150, 250, 350 mm. The fragile nature of coating cross-sections suggests that the typical lamellar structure of zirconia is replaced by a transgranular structure. Nonetheless, apparent porosity, ionic conductivity, open circuit voltage, and ohmic resistance measurements indicate that VLPPS is a viable method for producing MS-SOFCs.
- Published
- 2019
27. Partial inhibition of activin receptor-like kinase 4 attenuates pressure overload-induced cardiac fibrosis and improves cardiac function
- Author
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Hong Wang, Qian Wang, Yue-Peng Wang, Jian-Wen Hou, Chang-Yi Li, Yi-He Chen, and Yi-Gang Li
- Subjects
Male ,0301 basic medicine ,Physiology ,Cardiac fibrosis ,Apoptosis ,Blood Pressure ,Haploinsufficiency ,Smad2 Protein ,030204 cardiovascular system & hematology ,Mice ,Ventricular Dysfunction, Left ,0302 clinical medicine ,Transforming Growth Factor beta ,Fibrosis ,Myocytes, Cardiac ,Aorta ,Cells, Cultured ,Ejection fraction ,biology ,Angiotensin II ,Cell Differentiation ,Activin receptor ,Up-Regulation ,Collagen ,Cardiology and Cardiovascular Medicine ,Signal Transduction ,Cardiac function curve ,Heterozygote ,medicine.medical_specialty ,Cardiomegaly ,03 medical and health sciences ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Smad3 Protein ,Cell Proliferation ,Pressure overload ,business.industry ,Myocardium ,Stroke Volume ,Transforming growth factor beta ,Fibroblasts ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,Heart failure ,biology.protein ,business ,Activin Receptors, Type I - Abstract
Background Activin receptor-like kinase 4 (ALK4), a downstream receptor of transforming growth factor-β superfamily, is highly expressed in the mammal heart. Upregulated ALK4 expression and activated ALK4-small mother against decapentaplegic (Smad)2/3 signaling have been reported to play a pivotal role in tumorigenesis and in the development of systemic sclerosis. However, the role of ALK4-Smad2/3 pathway in the pathogenesis of cardiac hypertrophy and cardiac fibrosis remains unknown. Methods and results In this study, the mice with heterozygous knocking out of ALK4 gene (ALK4) were generated and subjected to aortic banding for 4 weeks. We found that ALK4 expression was upregulated in aortic banding-induced model of cardiac hypertrophy and cardiac fibrosis in wild-type mice. Compared with the wild-type mice, ALK4mice demonstrated a similar extent of aortic banding-induced cardiac hypertrophy, but a significant suppression of cardiac fibrosis to 64.8% of the basal level, and a subsequent amelioration in the cardiac dysfunction (left ventricle ejection fraction: 59.0 ± 6.4 in wild-type mice vs. 75.6 ± 3.9% in ALK4 mice; left ventricle end-diastolic pressure: 16.6 ± 4.7 mmHg in wild-type mice vs. 6.6 ± 2.8 mmHg in ALK4 mice) associated with inhibition of cardiac fibroblast activation and cardiomyocyte apoptosis. In vitro, ALK4 haploinsufficiency blocked the cellular proliferation/differentiation and collagen production in cultured cardiac fibroblasts after angiotensin-II stimulation. Mechanistically, ALK4 haploinsufficiency resulted in the suppression of Smad2/3 activity. Conclusion Our results demonstrate that ALK4 haploinsufficiency ameliorates cardiac fibrosis and dysfunction in a mouse pressure-overload model associated with inhibition of cardiac fibroblast activation and cardiomyocyte apoptosis through the suppression of Smad2/3 activity, and suggest that ALK4 is a novel therapeutic target in treating pressure overload-induced cardiac remodeling and heart failure.
- Published
- 2016
28. Performance evaluation of highly active and novel La0.7Sr0.3Ti0.1Fe0.6Ni0.3O3-δ material both as cathode and anode for intermediate-temperature symmetrical solid oxide fuel cell
- Author
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Chang-Jiu Li, Yue-Peng Wang, Shan-Lin Zhang, Jiu-Tao Gao, Kausar Shaheen, Muhammad Yasir, Cheng-Xin Li, and Muhammad Bilal Hanif
- Subjects
Materials science ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,02 engineering and technology ,Conductivity ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Polaron ,01 natural sciences ,Cathode ,0104 chemical sciences ,Anode ,law.invention ,Chemical engineering ,law ,Energy transformation ,Solid oxide fuel cell ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,0210 nano-technology ,Polarization (electrochemistry) ,Diffractometer - Abstract
In comparison to various power generating devices/energy conversion systems, solid oxide fuel cell (SOFC) has received much attention due to its remarkable efficiency, reliability and low pollution. Modified pechini method is used to synthesize La0.7Sr0.3Ti0.1Fe0.6Ni0.3O3-δ (LSTFN) perovskite material and is utilized as cathode and anode for intermediate-temperature symmetrical solid oxide fuel cell (IT-SSOFC). The X-ray diffractometer (XRD) patterns reveals perovskite cubic structure with single phase for LSTFN. The maximum conductivity ~318 Scm−1 is obtained for LSTFN in air at a temperature of 700 °C, but is decreased with further increase in temperature due to small polaron mechanism. However, the conductivity is enhanced up to ~1.1 Scm−1 at the same temperature in the presence of dry H2, being capable to be utilized both as anode and cathode. Low polarization resistance (Rp) ~0.047 (with air) and 0.201Ωcm2 (with dry H2) is exhibited by as prepared perovskite material at a temperature of 800 °C. The fabricated symmetrical cell maintains good stability with no apparent degradation during the tested hours ~300 in air and ~100 with dry H2.
- Published
- 2020
29. Structured La0.6Sr0.4Co0.2Fe0.8O3-δ cathode with large-scale vertical cracks by atmospheric laminar plasma spraying for IT-SOFCs
- Author
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Guan-Jun Yang, Hui-Yu Zhang, Yue-Peng Wang, Cheng-Xin Li, Sen-Hui Liu, Shan-Lin Zhang, and Chang-Jiu Li
- Subjects
Materials science ,Mechanical Engineering ,Metals and Alloys ,Oxide ,Atmospheric-pressure plasma ,Laminar flow ,02 engineering and technology ,Plasma ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Cathode ,0104 chemical sciences ,law.invention ,Plasma arc welding ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,law ,Materials Chemistry ,Particle size ,Composite material ,0210 nano-technology ,Polarization (electrochemistry) - Abstract
La0.6Sr0.4Co0.2Fe0.8O3-δ (LSCF) is a promising cathode material for solid oxide fuel cells (SOFCs). Hence, structured LSCF coatings were deposited in this study by atmospheric laminar plasma spraying (ALPS) at the spraying distances from 150 mm to 250 mm. Particles with high temperature were obtained at low plasma arc power of only 15 kW. The microstructural characterization of the coatings showed cluster-like morphologies on the top surfaces and columnar features with large-scale vertical cracks at the cross section. An evolution mechanism of the coatings was proposed based on particle size. The movement of particles during laminar plasma jet depended on particle size, which was studied experimentally and by simulation to explain the selective deposition. The coatings showed lower polarization resistance when compared to LSCF coatings deposited by atmospheric plasma spraying (APS) due to large vertical crack densities. The high output performance of the SOFCs with the LSCF cathode also proved this phenomenon. The results suggest that ALPS provides extensive options for SOFCs manufacturing.
- Published
- 2020
30. Activin Receptor‐Like Kinase 4 Haplodeficiency Mitigates Arrhythmogenic Atrial Remodeling and Vulnerability to Atrial Fibrillation in Cardiac Pathological Hypertrophy
- Author
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Yudong Fei, Yi-He Chen, Yi-Gang Li, Daoliang Zhang, Qian Wang, Yue-Peng Wang, Jian-Wen Hou, Xiaoqing Chen, and Chang-Yi Li
- Subjects
Male ,0301 basic medicine ,Patch-Clamp Techniques ,Haploinsufficiency ,Smad2 Protein ,030204 cardiovascular system & hematology ,ALK4 ,Connexins ,Muscle hypertrophy ,Mice ,0302 clinical medicine ,Atrial Fibrillation ,Arrhythmia and Electrophysiology ,Myocytes, Cardiac ,Aorta, Abdominal ,Chemokine CCL2 ,Original Research ,Receptor like kinase ,Kinase ,cardiac hypertrophy ,Atrial fibrillation ,Middle Aged ,Electrophysiology ,Hypertension ,cardiovascular system ,Ventricular pressure ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,Atrial Remodeling ,medicine.medical_specialty ,Cardiomegaly ,Smad2/3 ,03 medical and health sciences ,Heart Conduction System ,Internal medicine ,medicine ,Animals ,Humans ,Genetic Predisposition to Disease ,Smad3 Protein ,Pathological ,Aged ,Inflammation ,Pressure overload ,Chemotactic Factors ,Interleukin-6 ,business.industry ,medicine.disease ,Fibrosis ,030104 developmental biology ,Connexin 43 ,structural and electrical remodeling ,business ,Activin Receptors, Type I - Abstract
Background Activin receptor‐like kinase 4 ( ALK 4) is highly expressed in mammal heart. Atrial fibrillation ( AF ) is closely related to ventricular pressure overload. Because pressure overload increases atrial pressure and leads to atrial remodeling, it would be informative to know whether ALK 4 exerts potential effects on atrial remodeling and AF vulnerability in a pressure‐overload model. Methods and Results Wild‐type littermates and ALK 4 +/− mice were subjected to abdominal aortic constriction or a sham operation. After 4 or 8 weeks, echocardiographic and hemodynamic measurements were performed, and inducibility of AF was tested. The hearts were divided into atria and ventricles and then were fixed in formalin for staining, or they were weighted and snap‐frozen for quantitative real‐time polymerase chain reaction and Western blot analysis. Compared with wild‐type littermates, ALK 4 +/− mice demonstrated a similar extent of atrial hypertrophy but significantly suppressed atrial fibrosis at 8 weeks post–abdominal aortic constriction. ALK 4 haplodeficiency partially blocked abdominal aortic constriction–induced upregulation of monocyte chemotactic protein 1 and interleukin‐6, and the increased chemotaxin of macrophages. ALK 4 haplodeficiency also blunted a reduction of connexin 40 and redistribution of connexin 43 from the intercalated disk to the lateral membranes, thereby improving localized conduction abnormalities. Meanwhile, ALK 4 haplodeficiency inhibited abdominal aortic constriction–induced decreased I N a , I C a‐L and I K 1 densities as well as the accompanying action potential duration shortening. Mechanistically, ALK 4 haploinsufficiency resulted in the suppression of Smad2/3 activity in this model. Conclusions Our results demonstrate that ALK 4 haplodeficiency ameliorates atrial remodeling and vulnerability to AF in a pressure‐overload model through inactivation of the Smad2/3 pathway, suggesting that ALK 4 might be a potential therapeutic target in combating pressure overload–induced AF .
- Published
- 2018
31. Inhibition of BRD4 attenuates transverse aortic constriction- and TGF-β-induced endothelial-mesenchymal transition and cardiac fibrosis
- Author
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Xin Zhao, Yi-Gang Li, Xuelian Wang, Zhen Li, Qi Wang, Qun-Shan Wang, Shuai Song, Ying Xiao, Long Chen, Yi Yu, Guojian Fang, Liang Liu, Wei Cao, Rui Zhang, and Yue-Peng Wang
- Subjects
0301 basic medicine ,Male ,Cardiac fibrosis ,Down-Regulation ,Neovascularization, Physiologic ,Cell Cycle Proteins ,Smad Proteins ,030204 cardiovascular system & hematology ,Umbilical vein ,Extracellular matrix ,Mesoderm ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,Transforming Growth Factor beta ,medicine ,Human Umbilical Vein Endothelial Cells ,Gene silencing ,Animals ,Humans ,Receptor ,Molecular Biology ,Aorta ,Extracellular Matrix Proteins ,Chemistry ,Myocardium ,Mesenchymal stem cell ,Nuclear Proteins ,Fibroblasts ,medicine.disease ,Constriction ,Fibrosis ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,Cardiology and Cardiovascular Medicine ,Receptors, Transforming Growth Factor beta ,Biomarkers ,Transforming growth factor ,Signal Transduction ,Transcription Factors - Abstract
Cardiac fibrosis (CF), a process characterized by potentiated proliferation of cardiac fibroblasts and excessive secretion and deposition of extracellular matrix (ECM) from the cells, contributes strongly to the pathogenesis of a series of cardiovascular (CV) diseases, including AMI, heart failure and atrial fibrillation. Endothelial-mesenchymal transition (EndMT), one of the sources of transformed cardiac fibroblasts, has been reported as a key factor involved in CF. However, the molecular basis of EndMT has not been thoroughly elucidated to date. At the posttranscriptional level, of the three epigenetic regulators, writer and eraser are reported to be involved in EndMT, but the role of reader in the process is still unknown. In this study, we aimed to explore the role of Bromodomain-containing protein 4 (BRD4), an acetyl-lysine reader protein, in EndMT-induced CF and related mechanisms. We found that BRD4 was upregulated in endothelial cells (ECs) in the pressure-overload mouse heart and that its functional inhibitor JQ1 potently attenuated the TAC-induced CF and preserved cardiac function. In umbilical vein endothelial cells (HUVECs) and mouse aortic endothelial cells (MAECs), bothJQ1 and shRNA-mediated silencing of BRD4 blocked TGF-β-induced EC migration, EndMT and ECM synthesis and preserved the EC sprouting behavior, possibly through the downregulation of a group of transcription factors specific for EndMT (Snail, Twist and Slug), the Smads pathway and TGF-β receptor I. In the absence of TGF-β stimulation, ectopic expression of BRD4 alone could facilitate EndMT, accelerate migration and increase the synthesis of ECM. In vivo, JQ1 also attenuated TAC-induced EndMT and CF, which was consistent with JQ1's intracellular mechanisms of action. Our results showed that BRD4 plays a critical role in EndMT-induced CF and that targeting BRD4 might be a novel therapeutic option for CF.
- Published
- 2018
32. Study on the Fabrication and Performance of Very Low Pressure Plasma Sprayed Large-Area Porous Metal Supported Solid Oxide Fuel Cell
- Author
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Jiu-Tao Gao, Cheng-Xin Li, Yue-Peng Wang, Shan-Lin Zhang, Guan-Jun Yang, and Chang-Jiu Li
- Abstract
High manufacturing costs and long-term degradation are the main problems that have become a “bottleneck” and impeded SOFC’s further development. It is well known that a high operating temperature is the major cause that leads to these problems. As such, reducing the operating temperature becomes a hotspot of research. It has been reported that a uniform and dense coating can be prepared by using very low pressure plasma spraying (VLPPS) technology. The current study focuses on VLPPS for application in large-area (~100 × 100 mm) porous metal supported solid oxide fuel cell (MSSOFC), especially for the preparation of the electrolyte. It was found that the densification of the electrolyte was very good, as confirmed by the open-circuit voltage (OCV) of the cell. In the temperature range of 550~750°C, the OCV of the cell stabilized between 1.05 V and 1.1 V. The power density of the cell was also measured.
- Published
- 2018
33. The transient receptor potential melastatin 4 channel inhibitor 9-phenanthrol modulates cardiac sodium channel
- Author
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Jian-Wen, Hou, Yu-Dong, Fei, Wei, Li, Yi-He, Chen, Qian, Wang, Ying, Xiao, Yue-Peng, Wang, and Yi-Gang, Li
- Subjects
Male ,HEK293 Cells ,Dose-Response Relationship, Drug ,Animals ,Humans ,TRPM Cation Channels ,Female ,Myocytes, Cardiac ,Rabbits ,Phenanthrenes ,Anti-Arrhythmia Agents ,Research Papers - Abstract
BACKGROUND AND PURPOSE: 9‐Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9‐phenanthrol. EXPERIMENTAL APPROACH: Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild‐type and mutated human recombinant SCN5A were expressed in HEK293 cells. Anemonia toxin II (ATX‐II) was used to amplify the late sodium current (I(NaL)) and induce arrhythmias. Whole‐cell patch clamp technique was used to record APs and ionic currents. KEY RESULTS: 9‐Phenanthrol (10–50 μM) stabilized ventricular repolarization and abolished arrhythmias induced by ATX‐II in both isolated VMs, PCs and wedge preparations. Further study revealed that 9‐phenanthrol modulated the gating properties of cardiac sodium channels and dose‐dependently inhibited I(NaL) and peak sodium current (I(NaP)) in VMs with an IC(50) of 18 and 71.5 μM respectively. Its ability to inhibit I(NaL) was further confirmed in PCs and HEK293 cells expressing SCN5A mutations. CONCLUSIONS AND IMPLICATIONS: Our results indicate that 9‐phenanthrol modulates the gating properties of cardiac sodium channels and inhibits I(NaL) and I(NaP), which may contribute to its antiarrhythmic and cardioprotective effects.
- Published
- 2018
34. Impact of phosphomimetic and non‐phosphorylatable mutations of phospholemman on L‐type calcium channels gating in<scp>HEK</scp>293T cells
- Author
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Yue-Peng Wang, Xiao-Meng Chen, Kai Guo, Zhi-Wen Zhou, Wei Li, Yibo Jiang, and Yi-Gang Li
- Subjects
Patch-Clamp Techniques ,Calcium Channels, L-Type ,L-type calcium channels ,Mutant ,Phospholemman ,phospholemman ,Gating ,Biology ,Cell Line ,Humans ,Myocytes, Cardiac ,L-type calcium channel ,Patch clamp ,Phosphorylation ,voltage-dependent inactivation ,Voltage-dependent calcium channel ,phosphorylation sites mutation ,fungi ,HEK 293 cells ,Membrane Proteins ,Original Articles ,Cell Biology ,Phosphoproteins ,Molecular biology ,Cell biology ,HEK293 Cells ,Mutation ,Molecular Medicine ,activation ,deactivation - Abstract
Background: Phospholemman (PLM) is an important phosphorylation substrate for protein kinases A and C in the heart. Until now, the association between PLM phosphorylation status and L-type calcium channels (LTCCs) gating has not been fully understood. We investigated the kinetics of LTCCs in HEK 293T cells expressing phosphomimetic or nonphosphorylatable PLM mutants. Methods: The LTCCs gating was measured in HEK 293T cells transfected with LTCC and wild-type (WT) PLM, phosphomimetic or nonphosphorylatable PLM mutants: 6263AA, 6869AA, AAAA, 6263DD, 6869DD or DDDD. Results: WT PLM significantly slowed LTCCs activation and deactivation while enhanced voltage-dependent inactivation (VDI). PLM mutants 6869DD and DDDD significantly increased the peak of the currents. 6263DD accelerated channel activation, while 6263AA slowed it more than WT PLM. 6869DD significantly enhanced PLM-induced increase of VDI. AAAA slowed the channel activation more than 6263AA, and DDDD accelerated the channel VDI more than 6869DD. Conclusions: Our results demonstrate that phosphomimetic PLM could stimulate LTCCs and alter their dynamics, while PLM nonphosphorylatable mutant produced the opposite effects.
- Published
- 2015
35. Sanguinarine inhibits Rac1b-rendered cell survival enhancement by promoting apoptosis and blocking proliferation
- Author
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Si-si Wei, Kai Guo, Ji-min Gao, Xun Wang, Wei Li, Li Ying, Yi-Gang Li, Hong Wang, Jun-li Duan, Qing Zhou, Pei An, Xian-jin Luo, Ying Yu, Yue-Peng Wang, and Gang Li
- Subjects
Benzophenanthridines ,rac1 GTP-Binding Protein ,Pharmacology ,Cell Survival ,Blocking (radio) ,Cell growth ,Chemistry ,HEK 293 cells ,Apoptosis ,General Medicine ,Isoquinolines ,Cell Line ,chemistry.chemical_compound ,HEK293 Cells ,Cell culture ,Cancer research ,Humans ,Original Article ,Pharmacology (medical) ,Sanguinarine ,Cell survival ,Cell Proliferation - Abstract
Small GTPase Rac1 is a member of the Ras superfamily, which plays important roles in regulation of cytoskeleton reorganization, cell growth, proliferation, migration, etc. The aim of this study was to determine how a constitutively active Rac1b regulated cell proliferation and to investigate the effects of the Rac1b inhibitor sanguinarine.Three HEK293T cell lines stably overexpressing GFP, Rac1-GFP or Rac1b-GFP were constructed by lentiviral infection. The cells were treated with sanguinarine (1 μmol/L) or its analogue berberine (1 μmol/L) for 4 d. Cell proliferation was evaluated by counting cell numbers and with a BrdU incorporation assay. The levels of cleaved PARP-89 (an apoptosis marker) and cyclin-D1 (a proliferative index) were measured using Western blotting.In 10% serum-containing media, overexpressing either Rac1 or Rac1b did not significantly change the cell proliferation. In the serum-starved media, however, the survival rate of Rac1b cells was significantly increased, whereas that of Rac1 cells was moderately increased. The level of cleaved PARP-89 was significantly increased in serum-starved Rac1 cells, but markedly reduced in serum-starved Rac1b cells. The level of cyclin-D1 was significantly increased in both serum-starved Rac1 and Rac1b cells. Treatment with sanguinarine, but not berberine, inhibited the proliferation of Rac1b cells, which was accompanied by significantly increased the level of PARP-89, and decreased both the level of cyclin-D1 and the percentage of BrdU positive cells.Rac1b enhances the cell proliferation under a growth-limiting condition via both anti-apoptotic and pro-proliferative mechanisms. Sanguinarine, as the specific inhibitor of Rac1b, is a potential therapeutic agent for malignant tumors with up-regulated Rac1b.
- Published
- 2014
36. I
- Author
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Jian-Wen, Hou, Wei, Li, Yu-Dong, Fei, Yi-He, Chen, Qian, Wang, Yue-Peng, Wang, and Yi-Gang, Li
- Subjects
Calcium Channels, L-Type ,Nifedipine ,Pyridines ,Action Potentials ,Triazoles ,Calcium Channel Blockers ,Sodium Channels ,Electrophysiological Phenomena ,Kinetics ,Potassium Channel Blockers ,Animals ,Myocytes, Cardiac ,Heart Atria ,Rabbits ,4-Aminopyridine ,Single-Cell Analysis ,Anti-Arrhythmia Agents ,Cells, Cultured ,Sodium Channel Blockers - Abstract
Rate-dependent repolarization (RDR) of action potential (AP) in cardiomyocyte plays a critical role in the genesis of arrhythmias and RDR in atrium has been linked with atrial fibrillation. However, detailed studies focusing on the role of RDR in rabbit atrium are scant. In this study, atrial cells were isolated from rabbit heart and rate-dependent property was explored in single atrial cell to elucidate the underlying mechanism. Our results indicated that rate-dependent prolongation was evident at the action potential duration at 20% (APD20) and 50% (APD50) repolarization but not at 90% repolarization (APD90) under control condition. Using transient outward potassium current (I
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- 2017
37. The crucial role of activin A/ALK4 pathway in the pathogenesis of Ang-II-induced atrial fibrosis and vulnerability to atrial fibrillation
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Chang-Yi Li, Peng-Pai Zhang, Qian Wang, Yue-Peng Wang, Yi-He Chen, Ying Yu, Yi-Gang Li, and Jie Chen
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Adult ,Male ,0301 basic medicine ,medicine.medical_specialty ,Atrial enlargement ,Physiology ,030204 cardiovascular system & hematology ,Pathogenesis ,Extracellular matrix ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Physiology (medical) ,Internal medicine ,Atrial Fibrillation ,medicine ,Animals ,Humans ,Heart Atria ,cardiovascular diseases ,Aged ,Mice, Knockout ,Pressure overload ,business.industry ,Angiotensin II ,Atrial fibrillation ,Middle Aged ,medicine.disease ,Pathophysiology ,Activins ,030104 developmental biology ,Endocrinology ,cardiovascular system ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Activin Receptors, Type I - Abstract
Atrial fibrosis, the hallmark of structural remodeling associated with atrial fibrillation (AF), is characterized by abnormal proliferation of atrial fibroblasts and excessive deposition of extracellular matrix. Transforming growth factor-β1 (TGF-β1)/activin receptor-like kinase 5 (ALK5)/Smad2/3/4 pathway has been reported to be involved in the process. Recent studies have implicated both activin A and its specific downstream component activin receptor-like kinase 4 (ALK4) in stimulating fibrosis in non-cardiac organs. We recently reported that ALK4 haplodeficiency attenuated the pressure overload- and myocardial infarction-induced ventricular fibrosis. However, the role of activin A/ALK4 in the pathogenesis of atrial fibrosis and vulnerability to AF remains unknown. Our study provided experimental and clinical evidence for the involvement of activin A and ALK4 in the pathophysiology of atrial fibrosis and AF. Patients with AF had higher activin A and ALK4 expression in atriums as compared to individuals devoid of AF. After angiotensin-II (Ang-II) stimulation which mimicked atrial fibrosis progression, ALK4-deficient mice showed lower expression of ALK4 in atriums, reduced activation of atrial fibroblasts, blunted atrial enlargement and atrial fibrosis, and further reduced AF vulnerability upon right atrial electrophysiological studies as compared to wild-type littermates. Moreover, we found that apart from the well-known TGF-β1/ALK5 pathway, the activation of activin A/ALK4/smad2/3 pathway played an important role in the pathogenesis of Ang-II-mediated atrial fibrosis and inducibility of AF, suggesting that targeting ALK4 might be a potential therapy for atrial fibrosis and AF.
- Published
- 2017
38. Larger rate dependence of late sodium current in cardiac Purkinje cells: A potential link to arrhythmogenesis
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Yi-Gang Li, Jian Sun, Zhi-Quan Wang, Ying Yu, Zhi-Wen Zhou, Qing Zhou, Yue-Peng Wang, Jian-Hua Yan, Peng-Pai Zhang, Kai Guo, Jian-Wen Hou, and Wei Li
- Subjects
0301 basic medicine ,Heart Ventricles ,Purkinje cell ,Ranolazine ,Action Potentials ,Voltage-Gated Sodium Channels ,030204 cardiovascular system & hematology ,Sodium current ,Afterdepolarization ,03 medical and health sciences ,chemistry.chemical_compound ,Purkinje Cells ,0302 clinical medicine ,Heart Rate ,Physiology (medical) ,medicine ,Repolarization ,Animals ,Myocytes, Cardiac ,Patch clamp ,Cells, Cultured ,business.industry ,Arrhythmias, Cardiac ,humanities ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Tetrodotoxin ,Biophysics ,Action potential duration ,Rabbits ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Background Purkinje cells (PCs) have a steeper rate dependence of repolarization and are more susceptible to arrhythmic activity than do ventricular myocytes (VMs). Late sodium current (I NaL ) is rate dependent and contributes to rate dependence of repolarization. Objective This study sought to test our hypothesis that PCs have a larger rate dependence of I NaL , contributing to their steeper rate dependence of repolarization and higher susceptibility to arrhythmic activity, than do VMs. Methods I NaL was recorded in isolated rabbit PCs and VMs with the whole-cell patch clamp technique. Action potential was examined using the microelectrode technique. Results Compared with VMs, PCs exhibited a significantly larger rate dependence of I NaL with a larger I NaL to basic cycle length (BCL) slope. Moreover, PCs had a larger rate dependence of I NaL decay and slower recovery kinetics. Interestingly, the larger rate dependence of I NaL matched to a steeper rate dependence of action potential duration (APD) in PCs. The I NaL blocker tetrodotoxin significantly blunted, while the I NaL enhancer anemone toxin (ATX-II) significantly increased, the rate dependence of I NaL and APD in PCs and VMs. In the presence of ATX-II, the rate dependence of I NaL in PCs was markedly larger than that in VMs, causing a much steeper rate dependence of APD in PCs. Accordingly, PCs exhibited greater rate-dependent electrical instability and were more prone to ATX-II–induced early afterdepolarizations, which were completely inhibited by the I NaL inhibitor ranolazine. Conclusion PCs have a significantly larger rate dependence of I NaL than do VMs because of distinctive I NaL decay and recovery kinetics, which contributes to their larger rate adaptation, and simultaneously predisposes them to a higher risk of arrhythmogenesis.
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- 2016
39. Application of regularization technique to variational adjoint method: A case for nonlinear convection–diffusion problem
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Sulin Tao and Yue-Peng Wang
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Variable coefficient ,Computational Mathematics ,Data assimilation ,Speedup ,Diffusion problem ,Applied Mathematics ,Mathematical analysis ,Initial value problem ,Nonlinear convection ,Regularization (mathematics) ,Mathematics - Abstract
A discrete assimilation system for a one-dimensional variable coefficient convection–diffusion equation is constructed. The variational adjoint method combined with the regularization technique is employed to retrieve the initial condition and diffusion coefficient with the aid of a set of simulated observations. Several numerical experiments are performed: (a) retrieving both the initial condition and diffusion coefficient jointly (Experiment JR), (b) retrieving either of them separately (Experiment SR), (c) retrieving only the diffusion coefficient with the iteration count increased to 800 (Experiment NoR-SR), and (d) retrieving only the diffusion coefficient with the consideration of a regularization term based on the Experiment NoR-SR (Experiment AdR-SR). The results indicate that within the limit of 100 iterations, the retrieval quality of the Experiment SR is better than those from the Experiment JR. Compared with the initial condition, the diffusion coefficient is a little difficult to retrieve, whereas we still achieve the desired result by increasing the iterations or integrating the regularization term into the cost functional for the improvement with respect to the diffusion coefficient. Further comparisons between the Experiment NoR-SR and AdR-SR show that the regularization term can really help not only improve the precision of retrieval to a large extent, but also speed up the convergence of solution, even if some perturbations are imposed on those observations.
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- 2011
40. Generalized solitary wave solutions for the Klein–Gordon–Schrödinger equations
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Da-Feng Xia and Yue-Peng Wang
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Balance (metaphysics) ,Klein–Gordon–Schrödinger equations ,Exact solution ,010102 general mathematics ,Solitary wave solution ,01 natural sciences ,010305 fluids & plasmas ,Schrödinger equation ,symbols.namesake ,Computational Mathematics ,Exact solutions in general relativity ,Computational Theory and Mathematics ,Homogeneous ,Modeling and Simulation ,Modelling and Simulation ,0103 physical sciences ,Exp-function method ,symbols ,0101 mathematics ,Nonlinear evolution ,Klein–Gordon equation ,Mathematics ,Mathematical physics - Abstract
Some new generalized solitary solutions of the Klein–Gordon–Schrödinger equations are obtained using the Exp-function method, which include some known solutions obtained by the F-expansion method and the homogeneous balance method in the open literature as special cases. It is shown that the Exp-function method is a straight, concise, reliable and promising mathematical tool for solving nonlinear evolution equations arising in mathematical physics.
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- 2009
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41. Analytical Solution of Nonlinear Barotropic Vorticity Equation
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Yue-peng Wang and Wei-hui Shi
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Physics ,Mechanical Engineering ,Mathematical analysis ,Thermodynamics ,Vorticity ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,Condensed Matter Physics ,Stability (probability) ,Nonlinear system ,Vorticity equation ,Mechanics of Materials ,Modeling and Simulation ,Initial value problem ,Barotropic vorticity equation ,Well posedness - Abstract
The C k (k ≥ 3) stability of nonlinear barotropic vorticity equation was proved. The necessary and sufficient conditions for the initial value problem to be well-posed were presented. Under the conditions of well-posedness, the corresponding analytical solution was also gained.
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- 2008
42. Analytical solution of basic equations set of atmospheric motion
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Yue-peng Wang, Chun Shen, and Wei-hui Shi
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Partial differential equation ,Mechanics of Materials ,Applied Mathematics ,Mechanical Engineering ,Mathematical analysis ,Structure (category theory) ,Stability (learning theory) ,Initial value problem ,Point (geometry) ,Computational problem ,Space (mathematics) ,Mathematics ,Analytic function - Abstract
On condition that the basic equations set of atmospheric motion possesses the best stability in the smooth function classes, the structure of solution space for local analytical solution is discussed, by which the third-class initial value problem with typicality and application is analyzed. The calculational method and concrete expressions of analytical solution about the well-posed initial value problem of the third kind are given in the analytic function classes. Near an appointed point, the relevant theoretical and computational problems about analytical solution of initial value problem are solved completely in the meaning of local solution. Moreover, for other type of problems for determining solution, the computational method and process of their stable analytical solution can be obtained in a similar way given in this paper.
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- 2007
43. Rac1b enhances cell survival through activation of the JNK2/c-JUN/Cyclin-D1 and AKT2/MCL1 pathways
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Jie Chen, Si-Si Wei, Yi-He Chen, Wei-Ping Xu, Gang Li, Yidong Wei, Qiqiang Jie, Li Ying, Yi-Gang Li, Qing Zhou, Yue-Peng Wang, and Hong Wang
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0301 basic medicine ,rac1 GTP-Binding Protein ,Rac1b ,Cell Survival ,RAC1 ,Rats, Sprague-Dawley ,03 medical and health sciences ,Cyclin D1 ,Downregulation and upregulation ,Cell Line, Tumor ,Medicine ,Animals ,Humans ,Mitogen-Activated Protein Kinase 9 ,JNK2 ,AKT2 ,business.industry ,Cell growth ,HEK 293 cells ,c-jun ,apoptosis ,Microarray Analysis ,3. Good health ,Cell biology ,Rats ,030104 developmental biology ,HEK293 Cells ,cell proliferation ,Oncology ,Apoptosis ,Myeloid Cell Leukemia Sequence 1 Protein ,Signal transduction ,business ,Proto-Oncogene Proteins c-akt ,Signal Transduction ,Research Paper - Abstract
Rac1b is a constitutively activated, alternatively spliced form of the small GTPase Rac1. Previous studies showed that Rac1b promotes cell proliferation and inhibits apoptosis. In the present study, we used microarray analysis to detect genes differentially expressed in HEK293T cells and SW480 human colon cancer cells stably overexpressing Rac1b. We found that the pro-proliferation genes JNK2, c-JUN and cyclin-D1 as well as anti-apoptotic AKT2 and MCL1 were all upregulated in both lines. Rac1b promoted cell proliferation and inhibited apoptosis by activating the JNK2/c-JUN/cyclin-D1 and AKT2/MCL1 pathways, respectively. Very low Rac1b levels were detected in the colonic epithelium of wild-type Sprague-Dawley rats. Knockout of the rat Rac1 gene exon-3b or knockdown of endogenous Rac1b in HT29 human colon cancer cells downregulated only the AKT2/MCL1 pathway. Our study revealed that very low levels of endogenous Rac1b inhibit apoptosis, while Rac1b upregulation both promotes cell proliferation and inhibits apoptosis. It is likely the AKT2/MCL1 pathway is more sensitive to Rac1b regulation.
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- 2015
44. Dual-specificity phosphatase 14 protects the heart from aortic banding-induced cardiac hypertrophy and dysfunction through inactivation of TAK1-P38MAPK/-JNK1/2 signaling pathway
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Chang-Yi Li, Qing Zhou, Yue-Peng Wang, Ling-Chao Yang, Yi-Gang Li, Yi-He Chen, Kai Guo, and Jian-Wen Hou
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0301 basic medicine ,MAPK/ERK pathway ,medicine.medical_specialty ,Physiology ,MAP Kinase Signaling System ,Transgene ,Phosphatase ,Molecular Sequence Data ,Cardiomegaly ,Mice, Transgenic ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,Internal medicine ,Dual-specificity phosphatase ,medicine ,Animals ,Humans ,Myocytes, Cardiac ,Cells, Cultured ,Heart Failure ,biology ,Base Sequence ,Angiotensin II ,medicine.disease ,MAP Kinase Kinase Kinases ,Rats ,Mice, Inbred C57BL ,030104 developmental biology ,Endocrinology ,HEK293 Cells ,Heart failure ,Case-Control Studies ,Knockout mouse ,biology.protein ,Dual-Specificity Phosphatases ,Mitogen-Activated Protein Kinase Phosphatases ,Signal transduction ,Cardiology and Cardiovascular Medicine - Abstract
Dual-specificity phosphatase 14 (Dusp14), an important negative modulator of mitogen-activated protein kinase (MAPK) signaling pathways, has been implicated in inflammatory immune response, cancers, cell differentiation and proliferation. The role of Dusp14 in chronic pressure overload-induced cardiac hypertrophy has not been explored. Here we have shown that Dusp14-/- knockout mice and cardiac-specific Dusp14 transgenic mice were generated and subjected to aortic banding (AB) for 4 weeks. Our results demonstrated that genetic loss of Dusp14 significantly aggravated cardiac hypertrophy, fibrosis, ventricular dilation and dysfunction, whereas transgenic cardiac-specific Dusp14 overexpression significantly attenuated AB-induced cardiac dysfunction and remodeling. In vitro, adenoviral overexpression of constitutive Dusp14 blocked angiotensin II-induced hypertrophic growth of cardiomyocytes, while Dusp14 knockdown led to opposite effects. Mechanistically, excessive phosphorylation of TAK1, P38MAPK and JNK1/2 was evidenced in Dusp14-/- knockout mice post-AB and inactivation of TAK1-P38MAPK and -JNK1/2 signaling using TAK1 inhibitor 5Z-7-ox shares similar antihypertrophic effect as Dusp14 overexpression. Moreover, we show that Dusp14 directly interacted with TAK1. Results from present experiments indicate that Dusp14 protects the heart from AB-induced cardiac hypertrophy and dysfunction possibly through inactivation of TAK1-P38MAPK/-JNK1/2 signaling pathway. Future studies are warranted to test the feasibility of overexpressing Dusp14 as a therapeutic strategy to attenuate cardiac hypertrophy and failure.
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- 2015
45. Antiarrhythmic effects and potential mechanism of WenXin KeLi in cardiac Purkinje cells
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Chang-Yi Li, Xiao-Meng Chen, Jing Zhao, Yi-Gang Li, Yi-He Chen, Bu-Chang Zhao, Hong Wang, Wei Li, Yue-Peng Wang, Kai Guo, and Jian-Wen Hou
- Subjects
0301 basic medicine ,Patch-Clamp Techniques ,chemistry.chemical_element ,Action Potentials ,030204 cardiovascular system & hematology ,Pharmacology ,Calcium ,Sodium current ,03 medical and health sciences ,Purkinje Cells ,0302 clinical medicine ,Heart Conduction System ,Physiology (medical) ,medicine ,Animals ,Wenxin keli ,Myocytes, Cardiac ,Patch clamp ,Potential mechanism ,Flecainide ,business.industry ,Arrhythmias, Cardiac ,Nap ,Electrophysiology ,030104 developmental biology ,chemistry ,Rabbits ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug ,Drugs, Chinese Herbal - Abstract
Background Previous studies have demonstrated that WenXin KeLi (WXKL), a traditional Chinese medicine, can exert antiarrhythmic properties through complex multichannel inhibition, but its pharmacologic effect remains to be elucidated, especially in the cardiac conductive system. Objective To explore the antiarrhythmic property of WXKL in cardiac Purkinje cells (PCs). Methods PCs were isolated from rabbit hearts and action potentials (APs) and ion currents were recorded by whole-cell patch clamp technique. Anemonia toxin II (ATX-II) and isoproterenol (ISO) were used to induce early or delayed afterdepolarizations (EADs, DADs) or triggered activities (TAs). Results WXKL (1 g/L and 5 g/L) significantly abbreviated the action potential duration (APD) of PCs in a dose- and rate-dependent manner. Treatment of PCs with ATX-II (2 nM) prolonged APD and induced EADs, which were significantly suppressed by WXKL. WXKL (1, 5 g/L) also inhibited ISO-induced EADs, DADs, and TAs. To reveal the ionic mechanisms, we studied the effects of WXKL on late sodium current (I NaL ), peak sodium current (I NaP ), and L-type calcium currents (I CaL ) in PCs. WXKL-attenuated ATX-II (5 nM) induced I NaL augmentation and blocked I NaL with an IC 50 of 4.3 ± 0.5 g/L, which is 3- to 4-fold more selective than that of I NaP (13.3 ± 0.9 g/L) and I CaL (17.6 ± 1.4 g/L). Moreover, WXKL exerted significantly less use-dependent block of I NaP than that of flecainide, indicating its lower proarrhythmic effect. Conclusions WXKL exhibits antiarrhythmic properties in cardiac PCs via selective inhibition of I NaL .
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- 2015
46. Effect of Salidroside on Cardiac Functional Recovery
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Liu Hang Wang, Zhong Hua Zheng, Yan Zhang, Yue Peng Wang, and Guo Liang Peng
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Cardiac function curve ,medicine.medical_specialty ,business.industry ,Salidroside ,General Engineering ,Rat heart ,Functional recovery ,chemistry.chemical_compound ,chemistry ,High dosage ,Internal medicine ,Cardiology ,medicine ,Thomas' solution ,business ,Coronary flow - Abstract
To investigate the cardioprotective effect of salidroside to rat heart subjected to 8-hour hypothermic storage and 2-hour normothermic reperfusion. Isolated rat hearts were perfused with Langendorff model; after 30 minutes of baseline, the hearts were arrested and stored by St. Thomas solution (STS) without (STS group) or with different concentration salidroside at 4 °C for 8 hours, then reperfused for 2 hours. Compared with STS group, both middle and high dosage in STS greatly improved the recovery of left ventricular developed pressure (LVDP), maximum LVDP increase and decrease rate (±dp/dt), coronary flow rate (CF). Our study demonstrated that the salidroside was beneficial to improving cardiac functional recovery.
- Published
- 2013
47. Study on the Fabrication and Performance of Very Low Pressure Plasma Sprayed Porous Metal Supported Solid Oxide Fuel Cell
- Author
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Yue-Peng Wang, Shan-Lin Zhang, Chang-Jiu Li, Guan-Jun Yang, Cheng-Xin Li, and Jiu-Tao Gao
- Subjects
Low pressure plasma ,Porous metal ,Materials science ,Fabrication ,Solid oxide fuel cell ,Nanotechnology - Abstract
Solid oxide fuel cell (SOFC) is a high efficiency clean device that directly converts chemical energy of the fuel gas into electric energy. The long-term degradation and stability are the main problems at high temperature, so how to reduce the working temperature becomes a hotspot of research. It is an important way to reduce the thickness of electrolyte. Therefore, how to produce dense and thin electrolyte films economically becomes particularly important. It has been reported that a uniform and dense coating is hopefully prepared by using very low pressure plasma spraying (VLPPS) technology. Due to low operating pressure (~100 Pa), the plasma plume length elongates and its diameter enlarges. Under this condition, the particles can been heated for more time, and it is helpful to form dense coatings. The current study focuses on this technology for application in porous metal supported solid oxide fuel cell (MS-SOFC), especially for preparation in electrolyte. Using SEM, it was found that the densification of the electrolyte was very well, and this could be confirmed by the open-circuit voltage (OCV) of the cell. In the temperature range of 550~750 ℃, the OCV of the cell fairly stabilized between 1.05 V and 1.1 V. The cell was also analyzed by using electrochemical impedance spectroscopy (EIS). The maximum power density of the cell could be up to more than 1200 mW/cm2 at 750 ℃. The long-term stability of the cell was also preliminarily explored, and the result has shown that it is a very promising method for application in MS-SOFC.
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- 2017
48. Oxidative Stress-Induced Afterdepolarizations and Protein Kinase C Signaling
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Qian Wang, Wei Li, Yi Gang Li, Yue Peng Wang, Xiao Meng Chen, Yu Dong Fei, Yi He Chen, Xiao Lei Xu, Jian Wen Hou, and Kai Guo
- Subjects
afterdepolarization ,0301 basic medicine ,medicine.medical_specialty ,Oxidative phosphorylation ,030204 cardiovascular system & hematology ,Pharmacology ,arrhythmia ,medicine.disease_cause ,Article ,Catalysis ,Membrane Potentials ,Protein kinase C signaling ,Afterdepolarization ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Myocytes, Cardiac ,Patch clamp ,Physical and Theoretical Chemistry ,Hydrogen peroxide ,lcsh:QH301-705.5 ,Protein Kinase Inhibitors ,Molecular Biology ,Cells, Cultured ,Protein Kinase C ,Spectroscopy ,Protein kinase C ,triggered activity ,Organic Chemistry ,General Medicine ,oxidative stress ,protein kinase C ,Computer Science Applications ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,Rabbits ,Perfusion ,Oxidative stress ,Signal Transduction - Abstract
Background: Hydrogen peroxide (H2O2)-induced oxidative stress has been demonstrated to induce afterdepolarizations and triggered activities in isolated myocytes, but the underlying mechanisms remain not fully understood. We aimed to explore whether protein kinase C (PKC) activation plays an important role in oxidative stress-induced afterdepolarizations. Methods: Action potentials and ion currents of isolated rabbit cardiomyocytes were recorded using the patch clamp technique. H2O2 (1 mM) was perfused to induce oxidative stress and the specific classical PKC inhibitor, Gö 6983 (1 μM), was applied to test the involvement of PKC. Results: H2O2 perfusion prolonged the action potential duration and induced afterdepolarizations. Pretreatment with Gö 6983 prevented the emergence of H2O2-induced afterdepolarizations. Additional application of Gö 6983 with H2O2 effectively suppressed H2O2-induced afterdepolarizations. H2O2 increased the late sodium current (INa,L) (n = 7, p < 0.01) and the L-type calcium current (ICa,L) (n = 5, p < 0.01), which were significantly reversed by Gö 6983 (p < 0.01). H2O2 also increased the transient outward potassium current (Ito) (n = 6, p < 0.05). However, Gö 6983 showed little effect on H2O2-induced enhancement of Ito. Conclusions: H2O2 induced afterdepolarizations via the activation of PKC and the enhancement of ICa,L and INa,L. These results provide evidence of a link between oxidative stress, PKC activation and afterdepolarizations.
- Published
- 2017
49. Mitogen-activated protein kinase phosphatase-1: a critical phosphatase manipulating mitogen-activated protein kinase signaling in cardiovascular disease (review)
- Author
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Yi-Gang Li, Kai Guo, Yue-Peng Wang, Chang-Yi Li, and Ling-Chao Yang
- Subjects
MAPK/ERK pathway ,biology ,MAP Kinase Signaling System ,Phosphatase ,Dual Specificity Phosphatase 1 ,General Medicine ,Cell cycle ,Cell biology ,Enzyme Activation ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Gene Expression Regulation ,Cardiovascular Diseases ,Mitogen-activated protein kinase ,Phosphoserine ,Genetics ,biology.protein ,Phosphorylation ,Animals ,Humans ,Tyrosine ,Protein kinase A - Abstract
Mitogen-activated protein kinase (MAPK) cascades are important players in the overall representation of cellular signal transduction pathways, and the deregulation of MAPKs is involved in a variety of diseases. The activation of MAPK signals occurs through phosphorylation by MAPK kinases at conserved threonine and tyrosine (Thr-Xaa-Tyr) residues. The mitogen-activated protein kinase phosphatases (MKPs) are a major part of the dual-specificity family of phosphatases and specifically inactivate MAPKs by dephosphorylating both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. MAPKs binding to MKPs can enhance MKP stability and activity, providing an important negative-feedback control mechanism that limits the MAPK cascades. In recent years, accumulating and compelling evidence from studies mainly employing cultured cells and mouse models has suggested that the archetypal MKP family member, MKP-1, plays a pivotal role in cardiovascular disease as a major negative modulator of MAPK signaling pathways. In the present review, we summarize the current knowledge on the pathological properties and the regulation of MKP-1 in cardiovascular disease, which may provide valuable therapeutic options.
- Published
- 2014
50. Effect of the angiotensin II receptor blocker valsartan on cardiac hypertrophy and myocardial histone deacetylase expression in rats with aortic constriction
- Author
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Jing‑Xiang Li, Yi‑Bo Jiang, Ying Yu, Wei‑Ping Xu, Tong‑Qing Yao, Yi‑Gang Li, Mao‑Zhen Zhang, and Yue‑Peng Wang
- Subjects
Cancer Research ,Angiotensin receptor ,medicine.medical_specialty ,Histone deacetylase 5 ,Oncogene ,business.industry ,Histone deacetylase 2 ,General Medicine ,Articles ,Brain natriuretic peptide ,Endocrinology ,Immunology and Microbiology (miscellaneous) ,Atrial natriuretic peptide ,Valsartan ,Apoptosis ,Internal medicine ,medicine ,business ,medicine.drug - Abstract
The aim of the present study was to observe the myocardial expression of members of the histone deacetylase (HDAC) family (HDAC2, HDAC5 and HDAC9) in rats with or without myocardial hypertrophy (MH) in the presence and absence of the angiotensin II receptor blocker valsartan. Adult male Wistar rats were randomly divided into three groups (n=6/group): Sham-operated control rats, treated with distilled water (1 ml/day) through gavage; rats with MH (established through aortic constriction), treated with distilled water (1 ml/day) through gavage; and MH + valsartan rats, treated with 20 mg/kg/day valsartan through gavage. Treatments commenced one day after surgery and continued for eight weeks. Body weight (BW), heart weight (HW) and plasma atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) levels were determined, and the myocardial expression of HDAC2, HDAC5 and HDAC9 was analyzed through a reverse transcription semi-quantitative polymerase chain reaction. The BWs of the rats in the three groups were similar at baseline; however, after eight weeks the BW of the rats in the MH + valsartan group was significantly reduced compared with that of the MH rats. Furthermore, the HW/BW ratio and plasma ANP and BNP levels were increased, the myocardial HDAC2 expression was significantly upregulated and the HDAC5 and HDAC9 expression was significantly downregulated in the MH rats compared with those in the control rats; however, these changes were significantly attenuated by valsartan. Modulation of myocardial HDAC5, HDAC9 and HDAC2 expression may therefore be one of the anti-hypertrophic mechanisms of valsartan in this rat MH model.
- Published
- 2014
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