Search

Since the global epidemic of COVID-19, China has faced a more complex and harsh international public opinion environment. Under this circumstance, China has actively strengthened the construction of national image, promoted the national mainstream media institutions represented by CGTN to settle in the international social networking platform, and extensively carried out official publicity activities including national image building. However, there is still a huge gap between China and other countries, such as Britain, the United States and Japan, in terms of communication power, influence and persuasion, and the communication effectiveness is far from what is expected. This paper takes the CGTN channel as the representative of China's official publicity media as the research object, and uses text analysis and content analysis to summarize and analyze CGTN channel's publicity purpose, reading and listening key data, etc. By horizontal comparison with the current mainstream channels in Britain and the United States, this paper sums up the unfavorable situation and main reasons CGTN channels face in terms of communication effectiveness and puts forward several suggestions on how to improve their communication effectiveness.

Monascus species are the producers of Monascus azaphilone pigments (MonAzPs) and lipid-lowering component Monacolin K, which have been widely used as food colorant and health products. In this study, silent information regulator 2 (Sir2) homolog (MrSir2) was characterized, and its impacts on the development and MonAzPs production of Monascus ruber were evaluated. Enzyme activity test in vitro showed that MrSir2 was an NAD

Theranostic agents based on inorganic nanomaterials are still suffered from the nonbiodegradable substances with long-term retention in body and unavoidable biological toxicity, as well as nonspecificity biodistribution with potential damage toward normal tissues. Here, we develop magnetic ions (Fe

Based on partial coherence theory, this study rigorously deduces the principle of spatial light interference microscopy (SLIM) and improves the calculation method of SLIM. The main problem we found with SLIM is that it simply defaults the phase of the direct light to 0. To address this problem, we propose and experimentally demonstrate a double four-step phase shift method. Simulation results show that this method can reduce the relative error of oil-immersed microsphere reconstruction to about 3.7%, and for red blood cell reconstruction, the relative error can be reduced to about 13%.

Traditional glioblastoma (GBM) cell lines do not maintain the heterogeneity of the original tumor, cell interactions, and therapy response, thus limiting their investigation in GBM theranostics. Herein, a kind of GBM tumor-targeting nanoparticles (NPs) TCFNP@iRGD are designed and constructed, which are generated by photoacoustic (PA) contrast agent 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-ylidene) malononitrile (TCF)-OH through facile nanoprecipitation and decorated with an active targeting ligand iRGD. Their potential in GBM detection via PA imaging on glioma patient-derived cells intracranial xenograft models is evaluated for the first time. Excellent tumor-specific PA mapping performance of GBM is realized by TCFNP@iRGD, demonstrating its promising potential in the clinical diagnosis of GBM.

We demonstrate additive driving force can induce logic and Set-Reset latch operation in a triple-well potential system and find the optimal parameter region with contour maps. The logic input signal plays the role of system bias. To be specific, the external driving force makes the system jump from high potential well to low potential well without jumping back, and thus can induce correct logic output. The problem that the optimal parameter region is too narrow can be solved in two ways: one is to increase the amplitude of the logic input signal, and the other is to introduce of logical vibration resonance. In addition, the logic input itself can be directly acted as the driving force to make the system in the correct potential well, that is, to achieve the correct logic and Set-Reset latch operation without other external driving force.

Background This study aimed at probing into the effect of long non-coding RNA (lncRNA) C-terminal binding protein 1 antisense RNA 2 (CTBP1-AS2) on gastric cancer (GC) cell proliferation and apoptosis, and its regulatory function on miR-139-3p and MMP11. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to examine the expressions of CTBP1-AS2, miR-139-3p and MMP11 mRNA in GC cell lines and clinical specimens. Cell counting kit-8 (CCK-8) assay, flow cytometry and EdU assay were conducted to examine the effects of CTBP1-AS2 and miR-139-3p on GC cell proliferation and apoptosis. Western blot was applied for detecting the expressions of Bax, Bcl-2 and MMP11. A lung metastasis mouse model was used to evaluate metastasis of GC cells in vivo. Bioinformatics, dual-luciferase report assay, RIP and RNA pull-down assays were utilized to validate the targeted relationship between CTBP1-AS2 and miR-139-3p as well as the targeting relationship between miR-139-3p and MMP11. Results CTBP1-AS2 was highly expressed in GC, and its high expression was strongly associated with increased TNM stage, increased tumor size and low degree of differentiation of the tumor tissues. Meanwhile, CTBP1-AS2 promoted GC cell proliferation, metastasis and suppressed apoptosis, while miR-139-3p could weaken these effects. In addition, CTBP1-AS2 was identified as a molecular sponge for miR-139-3p, and MMP11 was verified as a target gene of CTBP1-AS2. CTBP1-AS2 could increase the expression of MMP11 via repressing miR-139-3p. Conclusion CTBP1-AS2 promotes GC cells and inhibits apoptosis by regulating the miR-139-3p/MMP11 molecular axis.

A novel method referred to as hyperbranched rolling circle amplification (HRCA) coupled with lateral flow dipstick (LFD) (HRCA-LFD) here was developed for specific, sensitive, rapid, and simple detection of Prorocentrum minimum. HRCA-LFD relies on a padlock probe (PLP) consisting of a common ligation sequence, two terminal sequences that complement the target DNA, and a manually designed detection probe (LFD probe). The two terminal sequences of the PLP were designed against the species-specific sites of the large subunit ribosomal DNA (LSU rDNA) D1-D2 region of P. minimum. The optimum parameters for HRCA were as follows: PLP concentration of 20 pM, ligation time of 30 min, ligation temperature of 59 °C, enzymic digestion time of 105 min, amplification time of 45 min, and amplification temperature of 58 °C. The HRCA-LFD displaying high specificity could accurately distinguish P. minimum from other microalgae. The detection limit of HRCA-LFD was as low as 1.42 × 10−7 ng μL−1 for genomic DNA, 1.03 × 10−7 ng μL−1 (approximately 27 copies) for recombinant plasmid containing the inserted LSU rDNA D1-D2, and 0.17 cells for crude DNA extract of P. minimum, which was consistently 100 times more sensitive than regular PCR. Interfering test suggested that the performance of HRCA-LFD is stable and would not be affected by other non-target species. The HRCA-LFD results of field samples that are comparable with microscopic examination confirmed that the developed method is competent for detection of target cells in field samples. In conclusion, the developed HRCA-LFD exhibiting stable performance is specific, sensitive, and rapid, which provides a good alternative to traditional microscopic examination for the detection of P. minimum cells in field samples.

The carbide phase is critical in high-carbon martensitic stainless steels (HCMSS) used for cutlery. In this study, carbide evolution during the austenitizing of different HCMSS (5Cr15MoV, 7Cr17MoV, 9Cr14MoV, and 9Cr18MoV) and its effect on the microstructure and mechanical properties are investigated. The statistical analysis of carbides suggests that the dissolution of small-sized secondary carbides ( 0.5 μm). Due to the influence of the elemental diffusion distance, the interfacial energy, and directional dissolution, small-sized carbides are more soluble than large-size carbides, which can be determined by the change of the ratio of Cr/Fe (wt.%) of carbides.

Introduction Long non-coding RNA (lncRNA) was reported to be a crucial regulator in cancer. In this work, our purpose is to explore the biolog ical roles of nuclear paraspeckle assembly transcript 1 (NEAT1) in gastric cancer (GC). Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect NEAT1 expression in GC cells and normal cells. GC cell behaviors after NEAT1 overexpression or downregulation were analyzed by Cell Counting Kit-8 assay, colony formation assay, wound-healing assay, and flow cytometry assay. Bioinformatic tools were used to analyze the significance of NEAT1 in GC. The involvement of microRNA-365a-3p (miR-365a-3p) and ATP -binding cassette subfamily C member 4 (ABCC4) in the biological roles of NEAT1 in GC progression was validated by luciferase activity reporter assay and rescue experiments. Results We found NEAT1 increased expression in both GC tissues and cells and correlated with poorer overall survival of cancer patients. We found NEAT1 overexpression promotes, while its knockdown inhibits GC cell proliferation, colony formation, invasion, and cell cycle progression in vitro. Mechanism analyses showed that NEAT1 serves as a ceRNA to upregulate ABCC4 expression via sponging miR-365a-3p. Conclusion In this study, we revealed a NEAT1/miR-365a-3p/ABCC4 triplet in GC progression, which may provide novel targeted therapy markers for GC.

Purpose This paper aims to investigate the correlation between wear behavior and microstructure evolution in friction-induced deformation layers (FDL) of 30CrMnSi steel, especially the role of strain-hardening induced by plastic deformation in FDL, which accordingly alters the wear behavior. Design/methodology/approach Dry sliding friction and wear behaviors of the 30CrMnSi steel against quenched and tempered GCr15 steel were studied using a pin-on-disc tester. The microstructure, hardness and plastic deformation of FDL were investigated. Findings It was found that the evolution of microstructure and strain-hardening induced by plastic deformation were occurred in the subsurface. When the microstructure, hardness and depth of the plastic deformation layer (PDL) reached a relatively steady state, the friction process transformed into stable-state stage. The wear loss and depth of the PDL was in dynamic equilibrium at stable wear stage. Originality/value In this paper, the correlation among the microstructure evolution, the strain-hardening and wear behavior were systemically analyzed. This paper could provide a theoretical reference for optimizing the microstructure and strain hardening properties of tribo-pairs materials.

Accurate treatment of photothermal therapy (PTT) is crucial to avoid the unnecessary injury of normal cells and tissues. Therefore the real-time temperature monitoring in the PTT process has drawn more and more attention in recent years. Herein, we designed and prepared one kind of lanthanide (Ln3+)-doped up-conversion nanocomposites with multi-functions, which can not only provide temperature feedback in PTT process, but also play the photodynamic therapy (PDT) function for the synergistic effect of tumor therapy. Based on NaYF4:Yb, Er up-conversion nanoparticles (UCNPs), mesoporous SiO2 was modified on the surface combined with photosensitizer Chlorin e6 (Ce6) molecules, which could be excited by red emission of Er3+ under the 980 nm laser. Cit-CuS NPs were further linked on the surface of the composite served as photothermal conversion agent, therefore, the temperature of the PTT site can be monitored by recording the ratio of I525/I545 of green emissions, especially within the physiological range. Based on the guidance obtained from spectral experiments, we further investigated the dual-modal therapy effect both in vitro and in vivo, respectively, and acquired decent results.

ObjectiveTo experimentally determine the involvement and mechanism of long non-coding RNA (lncRNA) HCP5 in the development of gastric cancer (GC).MethodsDetection of HCP5, miR-186-5p, and WNT5A expression in clinical GC tissues and adjacent healthy tissues was performed, followed by Pearson correlation analysis. BGC-823 and AGS cells, with interferences of HCP5, miR-186-5p, and WNT5A, were cultured under hypoxia. MTT, colony formation assay, Caspase-3 activity assay, and transwell assay were applied for the determination of cell proliferation, viability, apoptosis, and invasion, respectively. Expressions of WNT5A and protein markers of epithelial-mesenchymal transition (EMT) in cells were detected by western blotting. And the binding of HCP5 and WNT5A to miR-186-5p was validated using dual-luciferase reporter assay.ResultsIn GC tissues, an increase in HCP5 and WNT5A expressions and a reduction in miR-186-5p expression were found, and the negative correlation between miR-186-5p and HCP5/WNT5A was proven. Subsequently, under hypoxia, an increase in HCP5 and WNT5A expressions and a decrease in miR-186-5p expression in GC cells were confirmed. In addition, in GC cells under hypoxia, the inhibition of HCP5 suppressed cell biological activity and EMT, while the inhibition of miR-186-5p or the overexpression of WNT5A led to the opposite changes.ConclusionAn upregulation of WNT5A expression by HCP5 competitively binding to miR-186-5p promotes GC cell development.

Background This study aimed at probing into the effect of long non-coding RNA (lncRNA) C-terminal binding protein 1 antisense RNA 2 (CTBP1-AS2) on gastric cancer (GC) cell proliferation and apoptosis, and its regulatory function on miR-139-3p and MMP11. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to examine the expressions of CTBP1-AS2, miR-139-3p and MMP11 mRNA in GC cell lines and clinical specimens. Cell counting kit-8 (CCK-8) assay, flow cytometry and EdU assay were conducted to examine the effects of CTBP1-AS2 and miR-139-3p on GC cell proliferation and apoptosis. Western blot was applied for detecting the expressions of Bax, Bcl-2 and MMP11. A lung metastasis mouse model was used to evaluate metastasis of GC cells in vivo. Bioinformatics, dual-luciferase report assay, RIP and RNA pull-down assays were utilized to validate the targeted relationship between CTBP1-AS2 and miR-139-3p as well as the targeting relationship between miR-139-3p and MMP11. Results CTBP1-AS2 was highly expressed in GC, and its high expression was strongly associated with increased TNM stage, increased tumor size and low degree of differentiation of the tumor tissues. Meanwhile, CTBP1-AS2 promoted GC cell proliferation, metastasis and suppressed apoptosis, while miR-139-3p could weaken these effects. In addition, CTBP1-AS2 was identified as a molecular sponge for miR-139-3p, and MMP11 was verified as a target gene of CTBP1-AS2. CTBP1-AS2 could increase the expression of MMP11 via repressing miR-139-3p. Conclusion CTBP1-AS2 promotes GC cells and inhibits apoptosis by regulating the miR-139-3p/MMP11 molecular axis.

The direct conversion of syngas into lower olefins is a highly attractive route for the synthesis of lower olefins. The selectivity of lower olefins via the conventional Fischer–Tropsch (FT) synthesis is restricted to ∼60% with high CH4 selectivity due to the limitation by the Anderson–Schulz–Flory (ASF) distribution. Here, we report the design of bifunctional catalysts for the direct conversion of syngas into lower olefins with selectivity significantly breaking the ASF distribution. The selectivity of C2–C4 olefins reached 87% at a CO conversion of 10% and was sustained at 77% by increasing CO conversion to 29% over a bifunctional catalyst composed of Zn-doped ZrO2 nanoparticles and zeolite SSZ-13 nanocrystals. The selectivity of CH4 was lower than 3% at the same time. It is demonstrated that the molar ratio of Zn/Zr, the density of Bronsted acid sites of SSZ-13 and the proximity of the two components play crucial roles in determining CO conversion and lower-olefin selectivity. Our kinetic studies indicate that methanol and dimethyl ether (DME) are key reaction intermediates, and the conversion of syngas to methanol/DME is the rate-determining step over the bifunctional catalyst. Formate and methoxide species have been observed on Zn-doped ZrO2 surfaces during the activation of CO in H2, and the formed methanol/DME are transformed into lower olefins in SSZ-13.

Detection and isolation of circulating tumor cells (CTCs) play a pivotal role in the diagnosis and prognosis of cancer, while the high capture efficiency and purity of CTCs are difficult to achieve simultaneously among the various isolation methods. In this work, we designed an inverted microchip integrating silicon nanowires (SiNWs) and multifunctional magnetic nanocomposites (Fe3O4@C6/Ce6@silane, Coumarin 6 (C6), Chlorin e6 (Ce6)) for enhanced capture efficiency and purity of CTCs. The Fe3O4@C6/Ce6@silane conjugated with antibody can label the CTCs and pull them to the upside SiNWs capture surface by the upward magnetic field with high purity. This inverted structure was also featured with real-time detection and photodynamic therapy (PDT) of CTCs with the confocal laser scanning microscope (CLSM). The results indicate the important role of the composites labels and the magnetic field, which greatly improves the capture purity of the CTCs to 90%. Meanwhile, capture efficiency of CTCs achieve to 90.3% in culture medium and 82% in blood with 2 mL/h flow rate, respectively. Based on the structure of the device and composites, the captured CTCs could be directly inactivated by the in situ photodynamic therapy in the capture process which holds positive impact to block cancer spread.

Cobalt nanoparticles were loaded onto mesoporous zeolite Na–Y (Na–meso-Y) by melt infiltration and impregnation methods. As compared to impregnation, melt infiltration resulted in Co nanoparticles with a narrower size distribution. The Co/Na–meso-Y catalyst prepared by the melt infiltration exhibited higher C10–C20 (diesel fuel) selectivity. The hydrogenolysis of heavier hydrocarbons was confirmed to occur under Fischer–Tropsch reaction conditions. Our studies for the hydrogenolysis of n-hexadecane revealed that the catalyst by the melt infiltration was more active and selective for the formation of C10–C15 hydrocarbons. We propose that the narrower Co size distribution favors the selective hydrogenolysis and thus the C10–C20 selectivity in Fischer–Tropsch synthesis. The addition of Mn with a proper content could further improve the diesel fuel selectivity to 65% by suppressing the formations of CH4 and lighter hydrocarbons. The Mn-modified Co/Na–meso-Y catalyst was very stable and no deactivation was obs...

Dry sliding tribological properties of normalized 40Cr steel pins against quenched and tempered GCr15 discs were investigated under various sliding distances through disc-on-pin contact configuration. Microstructural feature evolution and strain-hardening behavior of worn subsurface in 40Cr pins were systematically analyzed using scanning electron microscopy and transmission electron microscopy. It is found that based on the variation of wear rate, three stages can be divided throughout the friction process: initial wear, slow wear and stable wear. The corresponding microstructures beneath contact surface are subjected to significant strain-hardening effect and structural rearrangement. Nano-grains of 40–100 nm are produced in the topmost subsurface layer and the nanocrystallization mechanism during friction process is further elucidated. Pronounced vortex structures are generated in the localized subsurface zones due to high strain localization and shear instability. With the prolongation of sliding distances, vortex structures suffer dynamic embrittlement and exfoliation from the wear surface as a result of periodically excessive hardening effect, leading to the high wear rate in stable wear stage.

A novel solvent-thermal Y2O3 template method was explored to synthesise NaYF4:Yb3+,Tm3+ IOPCs, which show highly improved UCL properties.

Background: Polyacrylic acid (PAA)–coated gold nanorods (GNRs) were prepared in this research, and then the structure, stability, temperature increment efficiency, and biocompatibility of GNRs@PAA were detected. Methods: It was demonstrated that GNRs@PAA coupled with an 808 nm laser had superior efficiency of hyperthermia therapy for MG63 human osteosarcoma cell. Results: The mechanism of photothermal therapy of GNRs@PAA was explored, and it was proved that damaged cell membrane and DNA integration caused cell apoptosis and death, and the cell apoptosis rate had been obviously promoted by in vitro photothermal therapy which exhibited time–dose dependence. Conclusion: The results demonstrated that the GNRs@PAA could be a promising candidate for phototherapeutic applications in human osteosarcoma.

This paper aims to develop a desirable EEG-based classification algorithm. For this purpose, the discrete wavelet transform was applied to denoise the EEG signals. Then, the brain’s left and right hand movement features were extracted from the denoised signals by the independent component analysis (ICA). Finally, the support vector machine (SVM) classifier was adopted to recognize and classify the movement of the left and right hand actions. The experimental results show that our method achieves the recognition accuracy of 89.5% and 90.6% respectively. The research findings provide a valuable reference for the future research into the BCI system.

Zn–ZrO2/SSZ-13 catalyzed direct conversion of syngas into lower olefins via methanol/DME intermediates with 77% selectivity at 29% CO conversion., The direct conversion of syngas into lower olefins is a highly attractive route for the synthesis of lower olefins. The selectivity of lower olefins via the conventional Fischer–Tropsch (FT) synthesis is restricted to ∼60% with high CH4 selectivity due to the limitation by the Anderson–Schulz–Flory (ASF) distribution. Here, we report the design of bifunctional catalysts for the direct conversion of syngas into lower olefins with selectivity significantly breaking the ASF distribution. The selectivity of C2–C4 olefins reached 87% at a CO conversion of 10% and was sustained at 77% by increasing CO conversion to 29% over a bifunctional catalyst composed of Zn-doped ZrO2 nanoparticles and zeolite SSZ-13 nanocrystals. The selectivity of CH4 was lower than 3% at the same time. It is demonstrated that the molar ratio of Zn/Zr, the density of Brønsted acid sites of SSZ-13 and the proximity of the two components play crucial roles in determining CO conversion and lower-olefin selectivity. Our kinetic studies indicate that methanol and dimethyl ether (DME) are key reaction intermediates, and the conversion of syngas to methanol/DME is the rate-determining step over the bifunctional catalyst. Formate and methoxide species have been observed on Zn-doped ZrO2 surfaces during the activation of CO in H2, and the formed methanol/DME are transformed into lower olefins in SSZ-13.

Protein kinase C has been shown to play a central role in the cardioprotection of ischemic preconditioning. However, the mechanism underlying PKC-mediated cardioprotection is not completely understood. Given that caveolae are critical for PKC signaling, we sought to determine whether hypoxic preconditioning promotes translocation and association of PKC isoforms with caveolin-3. A cellular model of hypoxic preconditioning from adult rat cardiac myocytes (ARCM) or H9c2 cells was employed to examine PKC isoforms by molecular, biochemical and cellular imaging analysis. Hypoxia was induced by incubating the cells in an airtight chamber in which O2 was replaced by N2 with glucose-free Tyrode's solution. Cells were subjected to hypoxic preconditioning with 10 minutes of hypoxia followed by 30 minutes of reoxygenation. Western blot data indicated that the band intensity for PKCϵ, PKCδ or PKCα, but not PKCβ and PKCζ was enhanced significantly by hypoxic preconditioning from the caveolin-enriched plasma membrane interactions. Immunoprecipitation experiments from the caveolin-enriched membrane fractions of ARCM showed that the level of PKCϵ, PKCδ and PKCα in the anti-caveolin-3 immunoprecipitates was also increased by hypoxic preconditioning. Further, our FRET analysis in H9c2 cells suggested that there is a minimum FRET signal for caveolin-3 and PKCϵ along cell peripherals, but hypoxic preconditioning enhanced the FRET signal, indicating a potential interaction between caveolin-3 and PKCϵ. And also treatment of the cells with hypoxic preconditioning led to a smaller amount of translocation of PKCϵ to the mitochondria than that to the membrane. We demonstrate that hypoxic preconditioning promotes rapid association of PKCϵ, PKCδ and PKCα with the caveolin-enriched plasma membrane microdomain of cardiac myocytes, and PKCϵ via direct molecular interaction with caveolin-3. This regulatory mechanism may play an important role in cardioprotection.

Artificial fractal structures have attracted considerable scientific interest in circulating tumor cells (CTCs) detection and capture, which plays a pivotal role in the diagnosis and prognosis of cancer. Herein, we designed a bionic TiO2 inverse opal photonic crystal (IOPC) structure for highly efficient immunocapture of CTCs by combination of a magnetic Fe3O4@C6@silane nanoparticles with anti-EpCAM (antiepithelial cell adhesion molecule) and microchannel structure. Porous structure and dimension of IOPC TiO2 can be precisely controlled for mimicking cellular components, and anti-EpCAM antibody was further modified on IOPC interface by conjugating with polydopamine (PDA). The improvement of CTCs capture efficiency reaches a surprising factor of 20 for the IOPC interface compared to that on flat glass, suggesting that the IOPCs are responsible for the dramatic enhancement of the capture efficiency of MCF-7 cells. IOPC substrate with pore size of 415 nm leads to the optimal CTCs capture efficiency of 92% wi...

In this work, a new photoelectrochemical biosensor based on Ag2S nanoparticles (NPs) modified macroporous ZnO inverse opals structure (IOs) was developed for sensitive and rapid detection of alpha fetal protein (AFP). Small size and uniformly dispersed Ag2S NPs were prepared using the Successive Ionic Layer Adsorption And Reaction (SILAR) method, which were adsorbed on ZnO IOs surface and frame work as matrix for immobilization of AFP. The composite structure of ZnO/Ag2S expanded the scope of light absorption to long wavelength, which can make full use of the light energy. Meanwhile, an effective matching of energy levels between the conduction bands of Ag2S and ZnO are beneficial to the photo-generated electrons transfer. The biosensors based on FTO (fluorine-doped tinoxide) ZnO/Ag2S electrode showed enough sensitivity and a wide linear range from 0.05 ng/mL to 200 ng/mL with a low detection limit of 8 pg/mL for the detection of AFP. It also exhibited high reproducibility, specificity and stability. The proposed method was potentially attractive for achieving excellent photoelectrochemical biosensor for detection of other proteins.

A critical obstacle in advanced colorectal cancer (CRC) treatment is the insufficient improvement on survival of conventional chemotherapy. Cancer stem cells are reported to be one of the crucial explanations. CD133 has been identified as a surface marker of CRC stem cells. Bispecific antibodies (BiAbs) targeting tumor-specific antigens are promising therapeutics for malignant diseases, yet that targeting CD133 produced by genetic engineering has not been published. In the current research, CD133 expression in primary CRC was detected by immunohistochemistry, and an asymmetric BiAb consisting of monomer of chimeric AC133 (mouse anti-human CD133 monoclonal antibody) and single chain of humanized OKT3 was developed to eradicate CD133-expressing tumor cells by arming activated T cells in vitro and in vivo. In immunohistochemical examination, CD133 overexpression (>50% of stained cells) frequency was significantly correlated with lymphatic invasion and clinical stage. The new molecular revealed dual-antigen-binding specificity to CD133 and CD3, its distinct structure not only facilitated the purification procedure but also conferred the antibody to ensure a longer and stronger cytotoxic activity. By arming activated T cells, the new antibody displayed impressive cytotoxicity toward CD133(high) but not CD133(low) CRC cells in vitro, produced amounts of cytokines (interferon-γ and granulocyte-macrophage colony-stimulating factor), and could inhibit tumor growth and retard tumor development in nonobese diabetic-severe combined immunodeficient mice without apparent toxicity. Taken together, the new BiAb possesses prosperities that support that the molecule has the potential of being a promising candidate of new therapeutics for CRC therapy.

It is necessary to communicate with women according to the female psychology to network marketing success.Taobao style is a dialogue between buyer and seller when they are doing business in Taobao e-shopping. Byanalyzing its language characteristics, where and how people use it, this paper points out that Taobao style is akind of feminine language strategy which can be used in sales, after sale service and evaluation management. Itcan help us in creating a good image of seller and in reducing disputes in the trading market.

Background: Colorectal cancer (CRC) is one of the leading causes of cancer-related death all over the world. Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. However its role in CRC is unclear. This study is to explore the correlation between the protein expression of RSK4 and clinical pathologic characteristics in colorectal tumors, which might serve as a prognostic determinant of colorectal cancers. Methods: Biopsies of 103 colorectal cancer and 46 matched adjacent noncancerous tissues were collected for analysis of RSK4 protein by immunohistochemistry. The correlation between RSK4 protein expression and the clinical pathological features of colorectal cancers were evaluated by Chi-square test and Fisher’s exact test. The survival rates were analyzed by the Kaplan-Meier method, and the relationship between prognostic factors and patient survival was analyzed by the Cox proportional hazard models. Results: RSK4 was conversely correlated with some pathological classifications (P0.05 for all categories) respectively. Furthermore, patients with high protein level of RSK4 showed prolonged overall survivals (P

In this letter, the hysteresis mechanism of organic thin-film transistors (OTFTs) with polyvinyl alcohol (PVA) as gate dielectric is studied. By examining OTFTs with a blocking polymer layer between gate and PVA or between channel and PVA, we confirm that the origin of the hysteresis is caused by the holes injected from the gate and/or from the channel and trapped in the interface of pentacene/PVA and the PVA bulk. A method is proposed to reduce the hysteresis. As a result, a pentacene OTFT with free-hysteresis and high mobility of 1.8 cm2/Vs is achieved by a triple-layer polymer dielectric at low-operating voltages.