Search

Additional file 1: Figure S1. Predicted secondary structure of tRNA genes in the Ancylostoma sp. mt genome, Table S1. Retrieved 18S rRNAs in species of Strongylida and Ascaridida from GenBank, Table S2. The mt genome from species in Strongylida, Table S3. Nucleotide composition (%) of PCGs, entire mt genome, and skew value of Ancylostoma sp.

Additional file 1: Fig. S1. Morphological observation of worm originated from wild pangolin. Fig. S2: Alignment and phylogenetic analysis of ITS2 region from worms of sample A and sample B. Fig. S3: PCR fragments amplified from uncertain regions of the two mitochondrial sequences. Fig. S4: Reads mapping result of the mt DNA of Raillietina sp. was visualized by IGV. Fig. S5: Sequences alignment of mt1 and mt2 of Raillietina sp. amplified by PCR using two different primers (JB11-JB12 and mt2-PCR). Fig. S6: Predicted secondary structure of 22 tRNAs of mt1 and mt2 of Raillietina sp. compared with Raillietina tetragona. Fig. S7: Putative secondary structure of non-coding regions (NCR1, NCR2) in mt1, mt2, and related species of R. tetragona predicted by mfolds. Table S1: Primers used for amplification of repeat and N-sequence/unidentified regions of mt1 and mt2 of Raillietina sp. Table S2: The retrieved 18S rRNA sequences of observed Raillietina sp. and species in the Cyclophyllidea, Diphyllobothriidae, and Schistosomatidae (Trematode as outgroup) for phylogenetic analysis. Table S3: The ITS2 sequences of cestode parasites retrieved from GenBank and sequences from two samples of current Raillietina sp. Table S4: The mt1 and mt2 of Raillietina sp. and the downloaded mt genome of 12 PCGs from the order of Cyclophyllidea, Pseudophyllidea, Diphyllobothriidae, and Schistosomatidae (outgroup). Table S5: The number of nucleotide deletions and insertions observed between mt1 and mt2 of Raillietina sp. Table S6: Nucleotide compositions (%) of PCGs, entire mt genome, transfer RNA, ribosomal RNA, and skew value of mt1 and mt2 of Raillietina sp.

Additional file 6: Table S6. Ligand binding ability of nematode FARs.

Additional file 3: Table S2. Comparison of gene and exon numbers of far in nematodes.

Additional file 1: Figures S1 to S15. FigS1 - Genus level changes in gene numbers of nematode FAR in different subclades. FigS2 - Sequence identity of FAR domain from nematodes in Clade III. FigS3 - Sequence identity of FAR domain from nematodes in Clade IV. FigS4 - Sequence identity of FAR domain from nematodes in Clade V. FigS5 - Protein Maximum Likelihood tree of FAR domain among nematodes. FigS6 - Gene structure of far from nematodes in Clade III. FigS7 - Maximum-Likelihood tree of 310 FAR proteins from nematodes in Clade IV. FigS8 - Tandem duplicated far gene in Strongyloides ratti, Strongyloides stercoralis, Strongyloides papillosus, Steinernema carpocapsae, Steinernema scapterisci, Steinernema feltiae, Steinernema glaseri, and Rhabditophanes sp. KR3021 from Clade IV. FigS9 - Gene structure and expression pattern of far in some plant-parasitic nematodes. FigS10 - Sequence identity and gene structure analyses of bacteria far. FigS11 - Genome colinearity in the CDS region of FAR domain between plant-parasitic nematodes and these bacteria. FigS12 - Genome colinearity in the CDS region of FAR domain among plant-parasitic nematodes, other nematodes from different clades, and these bacteria. FigS13 - Protein Maximum Likelihood tree of FARs from nematodes in Clade V. FigS14 - Intron analysis of tandem duplicated C. elegans far. FigS15 - Gene locus of tandem duplicated far gene in Pristionchus exspectatus, Ancylostoma ceylanicum, Ancylostoma caninum, and Haemonchus contortus from Clade V. FigS16 - Relative fluorescence intensity of AcFAR-1 and AcFAR-3 binding with DAUDA.