35 results on '"Yu-Hung Wang"'
Search Results
2. Higher RUNX1 expression levels are associated with worse overall and leukaemia‐free survival in myelodysplastic syndrome patients
- Author
-
Yu‐Hung Wang, Chi‐Yuan Yao, Chien‐Chin Lin, Chi‐Ling Chen, Chia‐Lang Hsu, Cheng‐Hong Tsai, Hsin‐An Hou, Wen‐Chien Chou, and Hwei‐Fang Tien
- Published
- 2022
3. High BM plasma S100A8/A9 is associated with a perturbed microenvironment and poor prognosis in myelodysplastic syndromes
- Author
-
Yu-Hung Wang, Chien-Chin Lin, Chi-Yuan Yao, Fabio Amaral, Shan-Chi Yu, Chein-Jun Kao, Pin-Tsen Shih, Hsin-An Hou, Wen-Chien Chou, and Hwei-Fang Tien
- Subjects
Hematology - Abstract
S100A8/A9 is a proinflammatory protein and plays an essential role in the pathogenesis of myelodysplastic syndromes (MDS) via the S100A8/A9-Toll-like receptors axis. While S100A8/A9 levels have been used as biomarkers in many inflammatory diseases, their clinical relevance has not been conclusively resolved in MDS. To address this, we used an enzyme-linked immunosorbent assay to quantify S100A8/A9 heterodimers in bone marrow (BM) plasma from 215 MDS patients and compared S100A8/A9 levels across patients with various disease risks and genotypes. S100A8/A9 levels correlated with ASXL1 variant allele frequencies significantly. Moreover, mutant ASXL1 with concurrent RUNX1, STAG2, ZRSR2, or EZH2 mutations was associated with higher S100A8/A9 levels. We further showed that higher S100A8/A9 independently predicted inferior leukemia-free survival and overall survival in MDS patients, irrespective of age, Revised International Prognostic Scoring System subgroups, and known detrimental mutations. Lastly, through deep-sequenced transcriptomic analysis, we demonstrated that higher S100A8/A9 in the BM intimated a perturbed microenvironment with enhanced myeloid-derived suppressor cell-mediated tumor immune escape signal, altered metabolism, and impairment in the functions and quantities of CD8+ T cells and NK cells. S100A8/A9 in the BM microenvironment may be a potential biomarker in the prognostication of MDS and target for novel therapy.
- Published
- 2023
4. Distinct genetic landscapes and their clinical implications in younger and older patients with myelodysplastic syndromes
- Author
-
Wan‐Hsuan Lee, Chien‐Chin Lin, Yu‐Hung Wang, Chi‐Yuan Yao, Yuan‐Yeh Kuo, Mei‐Hsuan Tseng, Yen‐Ling Peng, Cheng‐An Hsu, Hsun‐I Sun, Yi‐Kuang Chuang, Chia‐Lang Hsu, Feng‐Ming Tien, Cheng‐Hong Tsai, Wen‐Chien Chou, Hsin‐An Hou, and Hwei‐Fang Tien
- Subjects
Cancer Research ,Oncology ,Hematology ,General Medicine - Abstract
Myelodysplastic syndromes (MDS) are a group of clinically and genetically diverse diseases that impose patients with an increased risk of leukemic transformation. While MDS is a disease of the elderly, the interplay between aging and molecular profiles is not fully understood, especially in the Asian population. Thus, we compared the genetic landscape between younger and older patients in a cohort of 698 patients with primary MDS to advance our understanding of the distinct pathogenesis and different survival impacts of gene mutations in MDS according to age. We found that the average mutation number was higher in the older patients than younger ones. The younger patients had more WT1 and CBL mutations, but less mutated ASXL1, DNMT3A, TET2, SF3B1, SRSF2, STAG2, and TP53 than the older patients. In multivariable survival analysis, RUNX1 mutations with higher variant allele frequency (VAF) and U2AF1 and TP53 mutations were independent poor prognostic indicators in the younger patients, whereas DNMT3A and IDH2 mutations with higher VAF and TP53 mutations conferred inferior outcomes in the older patients. In conclusion, we demonstrated the distinct genetic landscape between younger and older patients with MDS and suggested that mutations impact survival in an age-depended manner.
- Published
- 2022
5. Thermal Error Prediction of Ball Screws Based on Partial Least Square Method with Consideration of Friction Works
- Author
-
Chien-Hsiang Hung, Meng-Shiun Tsai, Mao-Qi Hong, Yu-Hung Wang, and Wen-Fang Chen
- Published
- 2022
6. The clinical and biological characterization of acute myeloid leukemia patients with S100A4 overexpression
- Author
-
Chi-Yuan Yao, Chien-Chin Lin, Yu-Hung Wang, Chia-Lang Hsu, Chein-Jun Kao, Hsin-An Hou, Wen-Chien Chou, and Hwei-Fang Tien
- Subjects
General Medicine - Abstract
The S100 family proteins are involved in a variety of important biological processes, most notably immune and inflammatory responses. Their dysregulation also plays a role in the pathogenesis of human cancers. S100A4, also known as metastasin, has long been regarded as a biological marker in tumor progression and metastasis in multiple solid cancers, but its clinical significance in acute myeloid leukemia (AML) has not been extensively studied.We retrospectively studied the association between S100A4 gene expression and the clinical characteristics, mutational and transcriptomic profiles of 227 AML patients treated with standard intensive chemotherapy. Genetic mutations of myeloid disease associated genes were analyzed by Sanger sequencing. Microarray-based transcriptomic gene expression profiling was performed on archived bone marrow mononuclear cells. Bioinformatic analyses, including differential gene expression and gene set enrichment analysis, were conducted to delineate the underlying pathogenic mechanisms.Higher S100A4 expression was associated with older age, monocytic differentiation of leukemic cells, and adverse clinical outcome. S100A4 high-expressors had inferior overall survival and disease-free survival; this finding could be validated in the TCGA AML cohort (both the microarray and RNA-seq platforms). Multivariate Cox regression analysis supported S100A4 as an independent prognostic factor. Bioinformatic analysis showed that AML with higher S100A4 expression was enriched for the interferon, NLRP3 inflammasome, and epithelial-mesenchymal transition pathways.This study provides evidence that S100A4 overexpression serves as a poor prognostic biomarker in AML, holds potential to guide treatment planning in the clinic, and indicates novel therapeutic directions.
- Published
- 2022
7. Immune signatures of bone marrow cells can independently predict prognosis in patients with myelodysplastic syndrome
- Author
-
Chi-Yuan Yao, Hsin-An Hou, Chia-Lang Hsu, Chien-Chin Lin, Yu-Hung Wang, Wen-Chien Chou, Cheng-Hong Tsai, and Hwei-Fang Tien
- Subjects
Male ,Oncology ,medicine.medical_specialty ,Biopsy ,Bone Marrow Cells ,Kaplan-Meier Estimate ,Gene mutation ,Immunophenotyping ,Pathogenesis ,Immune system ,Bone Marrow ,Internal medicine ,medicine ,Humans ,Aged ,Cluster of differentiation ,business.industry ,Proportional hazards model ,Gene Expression Profiling ,Myelodysplastic syndromes ,High-Throughput Nucleotide Sequencing ,Hematology ,Middle Aged ,Prognosis ,medicine.disease ,Cell Transformation, Neoplastic ,medicine.anatomical_structure ,International Prognostic Scoring System ,Myelodysplastic Syndromes ,Mutation ,Female ,Disease Susceptibility ,Bone marrow ,business ,Biomarkers - Abstract
Increasing evidence supports the role of the immune microenvironment and associated signalling in the pathogenesis of myelodysplastic syndromes (MDS). Nevertheless, the clinical relevancy of immune signals in patients with MDS remains elusive. To address this, we used single-sample gene-set enrichment analysis to score immune signatures of bone marrow (BM) samples from 176 patients with primary MDS. Enhanced signatures of 'immature dendritic cells' and 'natural killer cells with cluster of differentiation (CD)56bright' were correlated with better overall survival (OS), whilst higher 'CD103+ signature' was associated with reduced survival. An MDS-Immune-Risk (MIR) scoring system was constructed based on the weighted sums derived from Cox regression analysis. High MIR scores were correlated with higher revised International Prognostic Scoring System (IPSS-R) scores and mutations in ASXL transcriptional regulator 1 (ASXL1), Runt-related transcription factor 1 (RUNX1), and tumour protein p53 (TP53). High-score patients had significantly inferior leukaemia-free survival (LFS) and OS than low-score patients. The prognostic significance of MIR scores for survival remained valid across IPSS-R subgroups and was validated in two independent cohorts. Multivariable analysis revealed that a higher MIR score was an independent adverse risk factor for LFS and OS. We further proposed a model with the combination of MIR score and gene mutations to be complementary to IPSS-R for the prognostication of LFS and OS of patients with MDS.
- Published
- 2021
8. Integrative Analysis of Blast Phase Chronic Myelomonocytic Leukemia Reveals Distinct Transformation Phenotypes Predictive of Drug Response
- Author
-
Kristian Gurashi, Yu-Hung Wang, Tim C.P Somervaille, Kiran Batta, and Daniel H Wiseman
- Subjects
Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
9. A Three Gene Leukemic Stem Cell Signature Score Is Robustly Prognostic in Chronic Myelomonocytic Leukemia
- Author
-
Yu-Hung Wang, Chi-Yuan Yao, Chien-Chin Lin, Kristian Gurashi, Fabio Amaral, Andres Jerez, Tim C.P Somervaille, Hsin-An Hou, Wen-Chien Chou, Kiran Batta, Hwei-Fang Tien, and Daniel H Wiseman
- Subjects
Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
10. Higher
- Author
-
Yu-Hung, Wang, Chi-Yuan, Yao, Chien-Chin, Lin, Chi-Ling, Chen, Chia-Lang, Hsu, Cheng-Hong, Tsai, Hsin-An, Hou, Wen-Chien, Chou, and Hwei-Fang, Tien
- Published
- 2022
11. The Concept Study of Knowledge Value Added in Museum Digital Archives
- Author
-
Yu-Hung Wang and Cheng-Wei Fan
- Subjects
Engineering education ,05 social sciences ,Digital Archives ,050301 education ,Library science ,Knowledge value ,Sociology ,0509 other social sciences ,050904 information & library sciences ,0503 education - Abstract
Museum digital collection should explore its past (object digitization), present (cultural relic interpretation) and future (knowledge value-added and management) practices and benefits. Therefore, it needs a new perspective. Its importance and the preservation and reuse of Museum digital resources are worthy of attention. Looking at the development of museums, when modern culture has rapidly progressed from analog to digital media, from atoms to the world of bits, the role of museums in collecting objects has changed accordingly. The technology of information communication will become more important for the creation, sharing and preservation of Museum cultural resources through the reproduction and reproduction of exhibits. Based on the basic concepts of digital collections, this study aims to provide a clear direction for the development of Museum digital collections by clarifying the concept of knowledge added value
- Published
- 2020
12. Correlative analysis of overall survival with clinical characteristics in 127 patients with mantle cell lymphoma: a multi-institutional cohort in Taiwan
- Author
-
Shan-Chi Yu, Yi-Tsung Yang, Tai-Chung Huang, Jih-Luh Tang, Yu-Hung Wang, Bor-Sheng Ko, and Ming Yao
- Subjects
Male ,Oncology ,medicine.medical_specialty ,Taiwan ,Lymphoma, Mantle-Cell ,CHOP ,Blastoid ,Transplantation, Autologous ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,immune system diseases ,hemic and lymphatic diseases ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Transplantation, Homologous ,Extranodal Involvement ,Cyclophosphamide ,Gastrointestinal Neoplasms ,Hematology ,biology ,business.industry ,Remission Induction ,Induction chemotherapy ,Middle Aged ,Prognosis ,medicine.disease ,biology.organism_classification ,Lymphoma ,Doxorubicin ,Vincristine ,030220 oncology & carcinogenesis ,Cohort ,Prednisone ,Female ,Mantle cell lymphoma ,business ,Stem Cell Transplantation ,030215 immunology - Abstract
Mantle cell lymphoma (MCL) is a B-cell non-Hodgkin lymphoma often with extranodal involvement at diagnosis, and yet how this feature correlates with survival awaits elucidation. To address this issue, a correlative analysis between clinical features of 127 MCL patients and their overall survival (OS) was conducted. In this cohort, the median age at MCL diagnosis was 62 years and 81% were males. Eighty-four percent of patients were Ann Arbor stage 4, and 15% were blastoid variants. In patients with gastrointestinal MCL, approximately 40% had gastric involvement. In treatment, CHOP-based induction chemotherapy was given to 61.1% of patients. One-third of patients undertook autologous stem cell transplant (SCT), and 4.7% had allogeneic SCT. The median OS was 82 months and well-stratified in MIPI risk groups. In the multivariate analysis for OS, blastoid variants and gastric involvement were both independent risk factors whereas auto-SCT had a protective effect. Overall, this study corroborated with the current understandings and international therapeutic standards for MCL. Auto-SCT associated with a better OS while allo-SCT remained an option for blastoid variants and those who failed Auto-SCT. Interestingly, patients with gastric involvement tended to have worse survival, a finding that spawns more studies to investigate the mechanism.
- Published
- 2020
13. A 4-gene leukemic stem cell score can independently predict the prognosis of myelodysplastic syndrome patients
- Author
-
Hwei-Fang Tien, Chia-Lang Hsu, Chien-Chin Lin, Yu-Hung Wang, Chi-Yuan Yao, Wen-Chien Chou, Cheng-Hong Tsai, and Hsin-An Hou
- Subjects
Oncology ,medicine.medical_specialty ,Subgroup analysis ,LAPTM4B ,Risk Factors ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,Risk factor ,Oncogene Proteins ,Myeloid Neoplasia ,business.industry ,Stem Cells ,Membrane Proteins ,Myeloid leukemia ,Hematology ,Prognosis ,medicine.disease ,Leukemia, Myeloid, Acute ,Leukemia ,Haematopoiesis ,International Prognostic Scoring System ,Myelodysplastic Syndromes ,Stem cell ,business - Abstract
Myelodysplastic syndrome (MDS) comprised a heterogeneous group of diseases. The prognosis of patients varies even in the same risk groups. Searching for novel prognostic markers is warranted. Leukemic stem cells (LSCs) are responsible for chemoresistance and relapse in leukemia. Recently, expressions of 17 genes related to stemness of LSCs were found to be associated with prognosis in acute myeloid leukemia patients. However, the clinical impact of LSC genes expressions in MDS, a disorder arising from hematopoietic stem cells, remains unclear. We analyzed expression profile of the 17 stemness-related genes in primary MDS patients and identified expression of 4 genes (LAPTM4B, NGFRAP1, EMP1, and CPXM1) were significantly correlated with overall survival (OS). We constructed an LSC4 scoring system based on the weighted sums of the expression of 4 genes and explored its clinical implications in MDS patients. Higher LSC4 scores were associated with higher revised International Prognostic Scoring System (IPSS-R) scores, complex cytogenetics, and mutations in RUNX1, ASXL1, and TP53. High-score patients had significantly shorter OS and leukemia-free survival (LFS), which was also confirmed in 2 independent validation cohorts. Subgroup analysis revealed the prognostic significance of LSC4 scores for OS remained valid across IPSS-R lower- and higher-risk groups. Furthermore, higher LSC4 score was an independent adverse risk factor for OS and LFS in multivariate analysis. In summary, LSC4 score can independently predict prognosis in MDS patients irrespective of IPSS-R risks and may be used to guide the treatment of MDS patients, especially lower-risk group in whom usually only supportive treatment is given.
- Published
- 2020
14. Rituximab/Bendamustine or Rituximab/Ifosfamide/Carboplatin/Etoposide as Second-Line Therapy for Relapsed/Refractory Diffuse Large B-Cell Lymphoma: A Real-World Analysis
- Author
-
Yu-Hung Wang, Ching-Yen Su, Li-Chin Chen, Ming Yao, and Bor-Sheng Ko
- Abstract
Rituximab/bendamustine (RB) and rituximab/ifosfamide/carboplatin/etoposide (R-ICE) are commonly used to treat relapsed/refractory diffuse large B-cell lymphoma (DLBCL), although their effectiveness has not been compared in real-world practice. This study evaluated data from DLBCL patients who relapsed after or were refractory to first-line therapy in an electronic health record (EHR)-derived database between 2011 and 2018. One hundred thirty-seven patients using RB and 270 patients using R-ICE were included for analysis. Transplantation after second-line therapy was considered a censored event in the time-to-event analyses. Patients in the RB group were older and had poorer performance status while there were no significant differences in stage, cell of origin, and double-/triple-hit subtypes. Relative to the R-ICE group, the RB group had significantly longer time-to-next-treatment (TTNT) and overall survival (OS). Subgroup analyses revealed that patients who were
- Published
- 2021
15. Stem Cell Transplant for Mantle Cell Lymphoma in Taiwan
- Author
-
Yu-Hung Wang, Ching-Yun Hsieh, Liang-Tsai Hsiao, Tung-Liang Lin, Yi-Chang Liu, Ming Yao, Tran-Der Tan, and Bor-Sheng Ko
- Subjects
Adult ,Multidisciplinary ,Hematopoietic Stem Cell Transplantation ,Taiwan ,Lymphoma, Mantle-Cell ,Middle Aged ,Transplantation, Autologous ,Treatment Outcome ,surgical procedures, operative ,immune system diseases ,hemic and lymphatic diseases ,Humans ,Transplantation, Homologous ,Neoplasm Recurrence, Local ,therapeutics ,human activities ,Retrospective Studies ,Stem Cell Transplantation - Abstract
Mantle cell lymphoma (MCL) is a B-cell lymphoma featuring an aggressive course and a progressive relapsing pattern. International guidelines recommend early consolidative autologous stem cell transplant (auto-SCT) for eligible patients while reserving allogeneic SCT (allo-SCT) as second-line therapy for refractory cases. Since data describing the implementation of transplants in the Asian population with MCL are limited, we aimed to analyze post-SCT outcomes of 99 MCL patients from the Taiwan Bone Marrow Transplant Registry database. The median age was 56 years, and 11% of the patients had blastoid variant MCL. Ninety-four patients received auto-SCT, while 13 patients received allo-SCT, eight of which received allo-SCT after failing auto-SCT. Before auto-SCT, 52% of the patients were in their first complete remission (CR1). Overall, 37 patients (39%) relapsed after auto-SCT. The median post-auto-SCT progression-free survival and overall survival (OS) were 43.6 months and not reached, respectively. Blastoid variant MCL, transplant not received in CR1, and disease progression within 12 months post-auto-SCT independently predicted inferior OS in multivariate analysis. The median post-allo-SCT OS was 74 months. Two patients (15%) died of MCL recurrence post-allo-SCT. Three patients with refractory diseases were salvaged with ibrutinib or venetoclax to allo-SCT. Treatment strategies incorporating novel agents warrant further optimization.
- Published
- 2021
16. Busulfan-containing conditioning regimens in allogeneic hematopoietic stem cell transplantation for acute lymphoblastic leukemia: A Taiwan observational study
- Author
-
Feng-Ming Tien, Xiu-Wen Liao, Jih-Luh Tang, Ming Yao, Huai-Hsuan Huang, Cheng-Hong Tsai, Jia-Hau Liu, Bor-Sheng Ko, and Yu-Hung Wang
- Subjects
Adult ,Cancer Research ,medicine.medical_specialty ,Allogeneic transplantation ,Cyclophosphamide ,medicine.medical_treatment ,Taiwan ,Graft vs Host Disease ,Hematopoietic stem cell transplantation ,law.invention ,Randomized controlled trial ,law ,Internal medicine ,Medicine ,Humans ,Transplantation, Homologous ,Prospective Studies ,Busulfan ,Retrospective Studies ,business.industry ,Hematopoietic Stem Cell Transplantation ,Total body irradiation ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Transplantation ,Regimen ,Oncology ,business ,medicine.drug - Abstract
Background Allogeneic stem cell transplantation (allo-HSCT) is the ultimate cure for acute lymphoblastic leukemia (ALL). Aim This study was performed to compare the outcomes of ALL patients receiving busulfan (Bu) with cyclophosphamide (Cy)-based or total body irradiation (TBI)-based regimen in a Chinese population. Methods We enrolled 224 adult patients with ALL who received allo-HSCT at National Taiwan University Hospital between 1997 and 2016. Results The median age at transplantation was 33 years. Before allo-HSCT, 75.9% of patients attained first or late complete remission. A total of 141 patients (62.9%) received Bu/Cy-based conditioning, either myeloablative (MA) or reduced-intensity stem cell transplantation (RIST), and 83 patients received a TBI-based regimen (MA-TBI). Patients receiving the MA-Bu regimen had longer relapse-free survival (RFS) than those receiving the MA-TBI regimen (median, 24.1 vs. 6.7 months, p = .044). There was no difference in overall survival (OS, MA-Bu vs. MA-TBI vs. RIST-Bu: 39.4 vs. 28.2 vs. 13.1 months, p = .276), treatment-related mortality (TRM), or incidences of grade 3-4 acute graft-versus-host disease (GvHD). Among patients receiving identical GvHD prophylactic regimens, there was no difference between MA-Bu and MA-TBI groups regarding the incidence of grade 3-4 acute GvHD, grade 2-4, and all-grade chronic GvHD. In subgroup analysis, patients receiving oral busulfan had comparable RFS and OS to the intravenous busulfan group (p = .436 and p = .236, respectively), but a higher TRM (25% vs. 9.8%, p = .016). In the multivariable analysis, disease status before allo-HSCT was the only risk factor impacting RFS and OS. Conclusion In summary, patients receiving Bu/Cy-based or TBI-based regimens as conditioning had similar results in terms of OS, TRM, and acute GvHD, whereas the use of myeloablative Bu/Cy resulted in a better RFS. A Bu-based regimen could be an alternative conditioning choice for patients who are ineligible to receive TBI. Prospective and randomized controlled trials are warranted to validate the long-term outcomes.
- Published
- 2021
17. Effects of water-saving education in Taiwan on public water knowledge, attitude, and behavior intention change
- Author
-
Mei-Chen Chang, Jia-Ru Liou, and Yu-Hung Wang
- Subjects
05 social sciences ,Geography, Planning and Development ,050301 education ,Water saving ,Sociology ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,Environmental economics ,0503 education ,01 natural sciences ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
This study aims to measure the changes in the water literacy of the subjects (N = 620) aged over 18 before and after participating in the water-saving activities. The results showed that there is a significant improvement in the water literacy of the subjects after the water-saving activities but with a medium-small effect size; there is no significant correlation between water knowledge and water attitude before or after the activities. In addition, there is a significant difference in water literacy regarding subjects' background variables, such as age, income, and household water expenses. In other words, the attitude and behavior related to the use of water could be easily influenced by different background variables. Due to the limitation of the museum being the study field, the post-tests were given immediately following the activities, therefore the improvements of the subjects' knowledge and attitudes may not be retained over time. The findings can serve as a reference for the Water Resources Department to promote water conservation education in the future.
- Published
- 2019
18. A CIBERSORTx-based immune cell scoring system could independently predict the prognosis of patients with myelodysplastic syndromes
- Author
-
Cheng-Hong Tsai, Chia-Lang Hsu, Yen-Ling Peng, Chein-Jun Kao, Chi-Yuan Yao, Yu-Hung Wang, Hsin-An Hou, Hwei-Fang Tien, Mei-Hsuan Tseng, Yuan-Yeh Kuo, Chien-Chin Lin, and Wen-Chien Chou
- Subjects
Oncology ,medicine.medical_specialty ,NPM1 ,Cell ,medicine.disease_cause ,Immune system ,Bone Marrow ,Internal medicine ,medicine ,Tumor Microenvironment ,Humans ,Leukemia ,Myeloid Neoplasia ,business.industry ,Myelodysplastic syndromes ,Hematology ,Immune dysregulation ,medicine.disease ,Prognosis ,medicine.anatomical_structure ,International Prognostic Scoring System ,Myelodysplastic Syndromes ,Cytogenetic Analysis ,Bone marrow ,Stem cell ,business ,Nucleophosmin - Abstract
Key Points CIBERSORTx analytical approach reveals M0, M2, and eosinophil fractions in the BM to be the most relevant prognostic factors in patients with MDS.High-risk immune cell scores correlate with NF-κB signaling, oxidative stress, and leukemic stem cell signature pathways., Visual Abstract, Aside from cell intrinsic factors such as genetic alterations, immune dysregulation in the bone marrow (BM) microenvironment plays a role in the development and progression of myelodysplastic syndromes (MDS). However, the prognostic implications of various immune cells in patients with MDS remain unclear. We adopted CIBERSORTx to estimate the relative fractions of 22 subtypes of immune cells in the BM of 316 patients with MDS and correlated the results with clinical outcomes. A lower fraction of unpolarized M0 macrophages and higher fractions of M2 macrophages and eosinophils were significantly associated with inferior survival. An immune cell scoring system (ICSS) was constructed based on the proportion of these 3 immune cells in the BM. The ICSS high-risk patients had higher BM blast counts, higher frequencies of poor-risk cytogenetics, and more NPM1, TP53, and WT1 mutations than intermediate- and low-risk patients. The ICSS could stratify patients with MDS into 3 risk groups with distinct leukemia-free survival and overall survival among the total cohort and in the subgroups of patients with lower and higher disease risk based on the revised International Prognostic Scoring System (IPSS-R). The prognostic significance of ICSS was also validated in another independent cohort. Multivariable analysis revealed that ICSS independently predicted prognosis, regardless of age, IPSS-R, and mutation status. Bioinformatic analysis demonstrated a significant correlation between high-risk ICSS and nuclear factor κB signaling, oxidative stress, and leukemic stem cell signature pathways. Further studies investigating the mechanistic insight into the crosstalk between stem cells and immune cells are warranted.
- Published
- 2021
19. Distinct clinical and biological characteristics of acute myeloid leukemia with higher expression of long noncoding RNA KIAA0125
- Author
-
Hsin-An Hou, Hwei-Fang Tien, Chi-Yuan Yao, Sze-Hwei Lee, Chia-Lang Hsu, Wen-Chien Chou, Cheng-Hong Tsai, Chien-Chin Lin, Sheng-Yu Hung, and Yu-Hung Wang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,Leukemic stem cell signatures ,Biology ,medicine.disease_cause ,Disease-Free Survival ,chemistry.chemical_compound ,Internal medicine ,Gene expression ,medicine ,Humans ,Clinical significance ,RNA, Neoplasm ,Aged ,Aged, 80 and over ,Mutation ,Hematology ,Acute myeloid leukemia ,Gene Expression Regulation, Leukemic ,Myeloid leukemia ,General Medicine ,Middle Aged ,KIAA0125 ,Survival Rate ,Haematopoiesis ,Leukemia, Myeloid, Acute ,medicine.anatomical_structure ,RUNX1 ,chemistry ,Long non-coding RNA ,Cancer research ,Female ,RNA, Long Noncoding ,Original Article ,Bone marrow ,Chemoresistance - Abstract
Expression of long non-coding RNA KIAA0125 has been incorporated in various gene expression signatures for prognostic prediction in acute myeloid leukemia (AML) patients, yet its functions and clinical significance remain unclear. This study aimed to investigate the clinical and biological characteristics of AML bearing different levels of KIAA0125. We profiled KIAA0125 expression levels in bone marrow cells from 347 de novo AML patients and found higher KIAA0125 expression was closely associated with RUNX1 mutation, but inversely correlated with t(8;21) and t(15;17) karyotypes. Among the 227 patients who received standard chemotherapy, those with higher KIAA0125 expression had a lower complete remission rate, shorter overall survival (OS) and disease-free survival (DFS) than those with lower expression. The prognostic significance was validated in both TCGA and GSE12417 cohorts. Subgroup analyses showed that higher KIAA0125 expression also predicted shorter DFS and OS in patients with normal karyotype or non-M3 AML. In multivariable analysis, higher KIAA0125 expression remained an adverse risk factor independent of age, WBC counts, karyotypes, and mutation patterns. Bioinformatics analyses revealed that higher KIAA0125 expression was associated with hematopoietic and leukemic stem cell signatures and ATP-binding cassette transporters, two predisposing factors for chemoresistance.
- Published
- 2020
20. The expression levels of long non-coding RNA KIAA0125 are associated with distinct clinical and biological features in myelodysplastic syndromes
- Author
-
Hsin-An Hou, Cheng-Hong Tsai, Sheng-Yu Hung, Hwei-Fang Tien, Yu-Hung Wang, Chien-Chin Lin, Chia-Lang Hsu, Wen-Chien Chou, and Chi-Yuan Yao
- Subjects
Oncology ,Neuroblastoma RAS viral oncogene homolog ,Adult ,Male ,medicine.medical_specialty ,Disease ,Biology ,Lower risk ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Internal medicine ,Gene expression ,medicine ,Humans ,Gene ,Aged ,Aged, 80 and over ,Myelodysplastic syndromes ,Hematology ,Middle Aged ,medicine.disease ,Prognosis ,Long non-coding RNA ,Gene Expression Regulation, Neoplastic ,International Prognostic Scoring System ,030220 oncology & carcinogenesis ,Myelodysplastic Syndromes ,Female ,RNA, Long Noncoding ,Transcriptome ,030215 immunology - Abstract
Long non-coding RNAs (lncRNAs) have important functions in cancer biology. Among them, lncRNA KIAA0125 is one of the genes proposed to play a critical role in leukaemia stem cell (LSC). In this study, we aimed to investigate the clinical relevance of the expression levels of lncRNA KIAA0125 in myelodysplastic syndromes (MDS), a disease with highly heterogeneous clinical and biological features. Using RNA arrays, we measured the expression of KIAA0125 in 176 primary MDS patients. We found that higher KIAA0125 expression was associated with higher risk MDS, based on the revised International Prognostic Scoring System (IPSS-R), mutations in ASXL1 and NRAS, and predicted poorer overall survival (OS) and leukaemia-free survival (LFS). Multivariate analysis revealed that higher KIAA0125 expression was an independent, unfavourable prognostic factor for OS and LFS, irrespective of IPSS-R and mutation status. Further global gene expression profile analysis suggested a close association of higher KIAA0125 expressions with LSC signatures. The expression of KIAA0125 may be a potential biomarker to guide the treatment choice in MDS patients, especially those with lower risk subtypes, in whom palliative treatment is usually used.
- Published
- 2020
21. Distinct clinical and biological characteristics of acute myeloid leukemia with higher expression of long noncoding RNA KIAA0125
- Author
-
Yu-Hung Wang, Wen-Chien Chou, Cheng-Hong Tsai, Chia-Lang Hsu, Chi-Yuan Yao, Sze-Hwei Lee, Sheng-Yu Hung, Hwei-Fang Tien, Hsin-An Hou, and Chien-Chin Lin
- Subjects
Mutation ,Myeloid leukemia ,Biology ,medicine.disease_cause ,Long non-coding RNA ,Haematopoiesis ,chemistry.chemical_compound ,medicine.anatomical_structure ,RUNX1 ,chemistry ,hemic and lymphatic diseases ,Gene expression ,medicine ,Cancer research ,Clinical significance ,Bone marrow - Abstract
Expression of long non-coding RNA KIAA0125 has been incorporated in various gene expression signatures for prognostic prediction in acute myeloid leukemia (AML) patients, yet its functions and clinical significance remain unclear. This study was aimed to investigate the clinical and biological characteristics of AML bearing different levels of KIAA0125. We profiled KIAA0125 expression levels in bone marrow cells from 347 de novo AML patients and found higher KIAA0125 expression was closely associated with FLT3-ITD, RUNX1, and DNMT3A mutations, and M1 subtype by the French-American-British classification, but inversely correlated with t(8;21) and t(15;17). Among the 227 patients who received standard chemotherapy, those with higher KIAA0125 expression had a lower complete remission rate, shorter overall survival (OS) and disease-free survival (DFS) than those with lower expression. The prognostic significance was validated in both TCGA and GSE12417 cohorts. Subgroup analyses showed that higher KIAA0125 expression also predicted shorter DFS and OS in patients with normal karyotype or non-M3 AML. In multivariable analysis, higher KIAA0125 expression remained an adverse risk factor independent of age, WBC counts, karyotypes, and mutation patterns. Bioinformatics analyses revealed that higher KIAA0125 expression was associated with hematopoietic and leukemic stem cell signatures and ATP-binding cassette transporters, two predisposing factors for chemoresistance.
- Published
- 2020
22. Effects of Far-Infrared Therapy on Foot Circulation Among Hemodialysis Patients With Diabetes Mellitus
- Author
-
Yuanmay Chang, Fang-Yu Cheng, Chieh-Yu Liu, Yu-Hung Wang, and Yann-Fen C Chao
- Subjects
Male ,medicine.medical_specialty ,Angiogenesis ,Infrared Rays ,medicine.medical_treatment ,Taiwan ,Diabetes Complications ,03 medical and health sciences ,Dilation (metric space) ,0302 clinical medicine ,Renal Dialysis ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Foot Injuries ,Aged ,Research and Theory ,business.industry ,Middle Aged ,medicine.disease ,030227 psychiatry ,Treatment Outcome ,Blood Circulation ,Cardiology ,Female ,Hemodialysis ,business ,030217 neurology & neurosurgery ,Foot (unit) - Abstract
Background: Far-infrared radiation (FIR) therapy improves vessel dilation, circulation, vessel endothelial function, and angiogenesis and reduces atherosclerosis. However, evidence of FIR therapy’s effects on foot circulation among diabetic patients undergoing hemodialysis is scarce. Aim: To determine whether FIR therapy improves foot circulation in diabetic patients undergoing hemodialysis. Design: Quasi-experimental. Methods: In June to November 2017, diabetic patients undergoing hemodialysis ( N = 58) at a hemodialysis center in northern Taiwan were divided into two groups: the experimental group ( n = 31) received FIR therapy to the bilateral dorsalis pedis artery (40 min/session, 3 times/week for 6 months) and the control group ( n = 27) received conventional dialysis care. Paired t test, independent samples t test, two-proportion Z test, and repeated-measures analysis of covariance were performed to compare changes from baseline to the end of the 6-month intervention between the groups. Results: Significant positive effects of FIR therapy on temperature, pulse, and blood flow of the dorsalis pedis artery were observed. Sensitivity to pain, tactility, and pressure also improved significantly in the experimental group. The Edinburgh Claudication Questionnaire revealed that the experimental group had reductions in subjective experiences of soreness, tingling, and coldness in the feet. Conclusion: The findings of significant improvements to objective and subjective measures of blood flow and neural function in the experimental group indicate that FIR therapy improves blood circulation to the feet. This therapy thus has great potential to be an effective adjuvant treatment for patients with diabetes mellitus undergoing hemodialysis.
- Published
- 2020
23. RUNX1 Expression Can be Complementary to RUNX1 Mutation in MDS Prognostication
- Author
-
Hsin-An Hou, Wen-Chien Chou, Chia-Lang Hsu, Hwei-Fang Tien, Yu-Hung Wang, Chi-Yuan Yao, and Chien-Chin Lin
- Subjects
Genetics ,chemistry.chemical_compound ,RUNX1 ,chemistry ,Expression (architecture) ,hemic and lymphatic diseases ,embryonic structures ,Immunology ,Mutation (genetic algorithm) ,Cell Biology ,Hematology ,Biology ,Biochemistry - Abstract
RUNX1 is a member of the core-binding factor family of transcription factors and is imperative for establishing definitive haematopoiesis. Mutated RUNX1 is an adverse risk factor for myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML). Meanwhile, high expression of RUNX1 is correlated with dismal prognosis in cytogenetically normal AML patients and critical for maintaining leukemic stem cells across AML genetic subgroups. However, the clinical relevancy of RUNX1 expression in MDS patients remains elusive. This study aimed to investigate the prognostic and biologic impacts of RUNX1 expression in MDS patients. We recruited 341 primary MDS patients who had enough bone marrow (BM) samples for RNA and next-generation sequencing. We first examined the difference in RUNX1 expression among patients with wild RUNX1 and N-terminal or C-terminal RUNX1 mutation. Among the 341 patients, 54 (15.8%) harboured RUNX1 mutation, 15 (27.8%) in N-terminal and 39 (72.2%), C-terminal. Patients with C-terminal RUNX1 mutant had higher RUNX1 expression than those with N-terminal RUNX1 mutant or wild RUNX1 (p Regarding survival, we first examined the impact of RUNX1 mutation on survival in this cohort. As expected, patients with mutated RUNX1 had significantly inferior leukaemia-free survival (LFS) and overall survival (OS) than those with unmutated RUNX1 (p=0.007, and p=0.008, respectively). We then explored the effects of RUNX1 expression on patients' survival. Patients with higher RUNX1 expression had significantly inferior LFS and OS than those with lower expression (both p Figure 1 Figure 1. Disclosures Tien: AbbVie: Honoraria; Celgene: Honoraria, Research Funding; Novartis: Honoraria. Chou: Abbvie: Honoraria, Other: Advisory Board, Research Funding; Celgene: Honoraria, Other: Advisory Board, Research Funding; IQVIA: Honoraria, Other: Advisory Board; Pfizer: Honoraria, Other: Advisory Board; Novartis: Honoraria, Other: Advisory Board; Bristol Myers Squibb: Honoraria, Research Funding; Kirin: Honoraria, Research Funding.
- Published
- 2021
24. Next-Generation Sequencing Minimal Residual Disease of Mantle Cell Lymphoma in Autologous Stem Cell Grafts and Its Implication on Tumor Recurrence
- Author
-
Tai-Chung Huang, Yu-Hsuan Yang, Jih-Luh Tang, Ming Yao, Bor-Sheng Ko, Yi-Kuang Chuang, Shan-Chi Yu, Yu-Hung Wang, and Yi-Tsung Yang
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Immunology ,Somatic hypermutation ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Minimal residual disease ,Lymphoma ,Transplantation ,Autologous stem-cell transplantation ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Mantle cell lymphoma ,Stem cell ,business ,Diffuse large B-cell lymphoma - Abstract
Background & Purpose The clinical course of mantle cell lymphoma (MCL) is often inflicted with tumor recurrence even though front-line autologous stem cell transplantation (ASCT) is the current standard of care. To elucidate the mechanism of post-transplant recurrence, this study aimed to interrogate the minimal residual disease (MRD) of MCL in the autologous grafts. Materials & Methods Paired samples of 17 MCL patients' lymphoma diagnostic FFPE specimens were analyzed in parallel with their harvested autologous stem cell grafts. Extracted genomic DNA was subjected to LymphoTrackⓇ Dx IGH/IGK assay coupled with Illumina MiSeq sequencer to characterize the post-recombination immunoglobulin VDJ sequences. Positivity of MRD was defined for any identical sequences identified both in the diagnostic FFPE and in the autologous graft DNA. As the control for recombined VDJ protein motif analysis, diagnostic FFPE samples from 23 patients with diffuse large B cell lymphoma (DLBCL) were analyzed with the same platform. Results Of the 17 patients undertaking autologous stem cell harvest, 11 patients achieved complete response and 6 were in partial response before stem cell harvest. Sixteen of these actually underwent transplantation while one died of disease before transplantation. MRD was detected via next-generation sequencing (NGS) in 5 patients' autologous grafts with variable MRD loads and recombined VDJ stereotypes (Table 1). VH3-21 was the most prominent stereotype (41%), followed by VH4-59 (14%). The median somatic hypermutation rate was 0.88% (range 0 - 5.17%). Interestingly, a 46-amino-acid domain in recombined VDJ sequences, which was hydroxyl and amine group-rich, differed between MCL and DLBCL: hydrophilic amino acids were enriched in 6 positions of this domain in MCL (p1%) correlated with shorter post-ASCT PFS and OS than those without MRD (medians, 1.9 months vs 27 months (p=0.024) in Figure 1c, and 11.9 months vs 66.8 months (p=0.001) in Figure 1d, respectively). Conclusions Identification of MRD in autologous grafts by deep-sequencing VDJ recombination helped stratify MCL patients' post-ASCT outcomes. Higher MRD loads correlated with inferior post-ASCT PFS and OS. The implication of recombined VDJ stereotypes and their impact on ASCT outcomes warrants further investigation. Disclosures Ko: Roche: Honoraria.
- Published
- 2020
25. Hepatitis B reactivation during treatment of tyrosine kinase inhibitors-Experience in 142 adult patients with chronic myeloid leukemia
- Author
-
Chien-Yuan Chen, Wang-Huei Sheng, Ja-Der Liang, Hwei-Fang Tien, Yu-Hung Wang, and Feng-Ming Tien
- Subjects
Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Hepatitis B virus ,Adolescent ,Taiwan ,Myelogenous ,Young Adult ,Internal medicine ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,medicine ,Humans ,Young adult ,Protein Kinase Inhibitors ,Aged ,Retrospective Studies ,Aged, 80 and over ,business.industry ,Incidence (epidemiology) ,Incidence ,Myeloid leukemia ,Retrospective cohort study ,Hematology ,Hepatitis B ,Middle Aged ,medicine.disease ,Prognosis ,Leukemia ,Female ,Virus Activation ,business ,Tyrosine kinase ,Follow-Up Studies - Published
- 2019
26. A Leukemic Stem Cell Signature-Based Scoring System Can Independently Predict the Prognosis of Myelodysplastic Syndrome Patients
- Author
-
Yu-Hung Wang, Hwei-Fang Tien, Wen-Chien Chou, Chien-Chin Lin, Chi-Yuan Yao, Chia-Lung Hsu, Hsin-An Hou, and Cheng-Hong Tsai
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Cytogenetics ,Myeloid leukemia ,Institutional review board ,medicine.disease ,Haematopoiesis ,Leukemia ,International Prognostic Scoring System ,Internal medicine ,Medicine ,Risk factor ,Stem cell ,business - Abstract
Background: Myelodysplastic syndrome (MDS) comprised a heterogeneous group of diseases. The prognosis of patients varies even in the same risk groups. Searching for novel prognostic markers is warranted. Leukemic stem cells (LSC) are responsible for chemoresistance and relapse in leukemia. Recently, expressions of 17 genes related to stemness of LSC were found to be associated with prognosis in acute myeloid leukemia patients. However, the clinical impact of LSC genes expressions on patients with MDS, a disorder arising from hematopoietic stem cells, remains unclear. Methods: We analysed expression profile of the 17 stemness-related genes in primary MDS patients and identified expressions of 4 genes were significantly correlated with overall survival (OS). We constructed an LSC4 scoring system based on the weighted sums of the four genes expressions and explored its clinical implications in MDS patients. Findings: Higher LSC4 scores were associated with higher revised international prognostic scoring system (IPSS-R) scores, complex cytogenetics, and mutations in RUNX1, ASXL1, ZRSR2, U2AF1, SRSF2, and TP53. High-score patients had significantly shorter OS and leukemia-free survival (LFS) which was also confirmed in two independent validation cohorts. Subgroups analysis revealed the prognostic significance of LSC4 scores for OS remained valid across IPSS-R lower- and higher-risk groups. Furthermore, higher LSC4 score was an independent adverse risk factor for OS and LFS. Interpretation: LSC4 score can independently predict prognosis in MDS patients irrespective of IPSS-R risks and may be used to guide the treatment of MDS patients, especially lower-risk group in whom usually only supportive treatment is given. Funding: The study was supported by grants from Ministry of Health and Welfare, and Science and Technology, Taiwan. Declaration of Interest: The authors declare that they have no competing interests. Ethical Approval: This study was approved by the Institutional Review Board of the NTUH.
- Published
- 2019
27. Comparison of the effectiveness of Taiwanese college and high school students participating in creative contests
- Author
-
Mei-Chen Chang, Jia-Ru Liou, and Yu-Hung Wang
- Subjects
Team composition ,05 social sciences ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,050301 education ,050109 social psychology ,0501 psychology and cognitive sciences ,Affect (psychology) ,Psychology ,CONTEST ,0503 education ,Education ,Research model - Abstract
This study investigated the college students (N = 725) and high school students (N = 585) that participated in the National Energy Technology Creative Contest (NETCC) of Taiwan. AMOS analysis was performed to establish a model of students’ performances in creative contests and t-test was conducted to analyze and compare the differences in variables between the two groups of students in the model. The findings of this study are as follows: (1) the research model fits the model of college and high school students’ performances in creative contests; (2) for college students (Model 1), their performances in creative contests are directly affected by team composition and knowledge variables, and team composition variables can also directly affect knowledge variables, (3) for high school students (Model 2), their performances in creative contest are directly affected by team composition and knowledge variables, but there is no direct correlation between team composition variables and knowledge variables, (4) comparison and analysis of the mean of the variables that affect the students’ performances in creative contests show that, for most variables, the mean of high school students is significantly higher than that of college students.
- Published
- 2020
28. QUANTIFYING SOMATIC HYPERMUTATION RATES AND IDENTIFYING IMMUNOGLOBULIN HEAVY CHAIN STEREOTYPES IN MANTLE CELL LYMPHOMA THROUGH NEXT-GENERATION SEQUENCING
- Author
-
Tai-Chung Huang, Ming Yao, Shan-Chi Yu, Yu-Hung Wang, J. Tang, and C. Chuang
- Subjects
Genetics ,Cancer Research ,Oncology ,medicine ,Immunoglobulin heavy chain ,Somatic hypermutation ,Mantle cell lymphoma ,Hematology ,General Medicine ,Biology ,medicine.disease ,DNA sequencing - Published
- 2019
29. Busulfan-Containing Conditioning Regimens in Allogeneic Hematopoietic Stem-Cell Transplantation for Acute Lymphoblastic Leukemia: Single Tertiary Center Experience in Taiwan
- Author
-
Jih-Luh Tang, Ming Yao, Yu-Hung Wang, Xiu-Wen Liao, and Bor-Sheng Ko
- Subjects
Transplantation ,medicine.medical_specialty ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,Hematology ,Hematopoietic stem cell transplantation ,Total body irradiation ,Gastroenterology ,03 medical and health sciences ,Regimen ,0302 clinical medicine ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Bone marrow ,Risk factor ,business ,Busulfan ,030215 immunology ,medicine.drug - Abstract
The allogeneic hematopoietic stem-cell transplantation (allo-HSCT) is viewed as the ultimate cure for acute lymphoblastic leukemia (ALL). An essential component of allo-HSCT is the conditioning regimen administered before the hematopoietic stem-cell infusion. This study is aimed to compare outcomes of ALL patients receiving either a busulfan with cyclophosphamide (BuCy) or a total body irradiation(TBI)-based regimen. We retrospectively analyzed data from National Taiwan University Hospital institute registry. We enrolled 224 adult patients with ALL who received allo-HSCT between 1997 and 2016 and compared outcomes of patients receiving either a BuCy-based or a TBI-based regimen. There were more B-cell ALL than T-cell ALL (67.8% vs 32.2%). A total of 41 patients (22.5%) carried the Philadelphia chromosome (Ph) while 38 patients (20.9%) carried complex karyotype. Before the allo-HSCT, 164 (73.2%) patients achieved first or late complete remission (CR). The main source of stem-cell was from peripheral blood (71%), followed by bone marrow (22.3%). Siblings comprised 54.9% of the donors. A total of 141 patients (63%) received BuCy-based conditioning, either myeloablative (MA) or reduced intensity stem cell transplantation (RIST) and 83 patients (37%) received TBI-based regimen. The mean dose of stem cell was 4.96 ± 2.42 × 106/Kg CD34+ cells. We use cyclosporine and mycophenolate mofetil for prophylaxis of graft-versus-host disease (GvHD). In results, patients received BuCy-based regimen performed as well as TBI-based group in terms of 5-year overall survival (47.5% vs 37.3%, p=0.115). As to 5-year relapse-free survival (RFS), BuCy group had slightly better result, especially the group received myeloablative conditioning (MA, Bu-based vs. MA, TBI-based, p=0.059), albeit the p value didni¦t reach significance, the curves went on departing ways. In this study, all regimen groups yielded similar treatment-related mortality (TRM), (MA, Bu-based vs RIST, Bu-based vs MA, TBI-based: 17% vs 10.9% vs 21.7%, p=0.308) and incidences of grade 3-4 acute GvHD at day+100, (p=0.445). Further, in patients with extramedullary disease, there was no difference among groups; and interestingly, BuCy group with MA conditioning seemed to yield better result than TBI group (p=0.072). By multi-variate analysis, disease status before allo-HSCT is the only risk factor impacting OS and RFS. In conclusion, patients received BuCy-based regimen or TBI-based regimen as conditioning had similar results in terms of OS, RFS, TRM, and GvHD.
- Published
- 2019
30. A Leukemic Stem Cell Signature-Based Scoring System for Predicting Prognosis in Myelodysplastic Syndrome Patients
- Author
-
Wen-Chien Chou, Cheng-Hong Tsai, Hwei-Fang Tien, Chien-Chin Lin, Yu-Hung Wang, Chi-Yuan Yao, and Hsin-An Hou
- Subjects
Oncology ,Chemotherapy ,medicine.medical_specialty ,Proportional hazards model ,business.industry ,medicine.medical_treatment ,Immunology ,Cytogenetics ,Myeloid leukemia ,Hematopoietic stem cell ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Haematopoiesis ,medicine.anatomical_structure ,Dysplasia ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Stem cell ,business - Abstract
Leukemic stem cells (LSCs) possess biological properties shared with normal hematopoietic stem cells. They are responsible for chemoresistance and relapse in acute myeloid leukemia (AML). Although myelodysplastic syndrome (MDS) has traditionally been regarded as a "hematopoietic stem cell disorder", the clinical and biological impact of LSCs on MDS patients are not well defined. To address this question, we used the Affymetrix HTA 2.0 microarray platform to profile 16 out of the 17 recently reported stemness genes (one of them, the ARHGAP22 gene was not included in our array) in our 160 adult primary MDS patients. The diagnoses were based on the 2016 World Health Organization (WHO) classification. Patients with antecedent chemotherapy or hematologic malignancies were excluded. Forty-one (25.6%) patients had MDS with single lineage dysplasia (MDS-SLD), 20 (12.5%) had MDS with ring sideroblasts (MDS-RS), 31 (19.4%) had MDS with multilineage dysplasia (MDS-MLD), 32 (20%) had MDS with excess blasts-1 (MDS-EB1) and 36 (22.5%), MDS-EB2. The risk distribution of the cohort was very-high risk, 15.3%; high risk, 21.3%; intermediate risk, 24.7%; low risk 34.7%; and very-low risk 4% according to the revised international prognosis scoring system (IPSS-R). We identified 4 genes (LAPTM4B, NGFRAP1, NYNRIN, and EMP1) whose expression levels were significantly correlated with overall survival (OS). An LSC4 score (0.731 x LAPTM4B - 1.259 x NGFRAP1 + 0.304 x NYNRIN + 0.231 x EMP1) was constructed based on the weighted sums derived from Cox regression analysis. Higher LSC4 scores were associated with higher IPSS-R scores, complex cytogenetics, and higher incidences of mutations of RUNX1, ASXL1, TP53, SRSF2 and ZRSR2. High-score patients had significantly higher 3-year AML transformation rate (36.3% vs 11.3%, P Disclosures No relevant conflicts of interest to declare.
- Published
- 2018
31. Experimental Study on Direct Displacement-Based Seismic Design of RC Columns
- Author
-
K. C. Chang, Yu-Hung Wang, and Yu-Yuan Lin
- Subjects
Capacity spectrum method ,Computer science ,business.industry ,Applied Mathematics ,Mechanical Engineering ,Seismic engineering ,Stiffness ,Structural engineering ,Displacement based ,Condensed Matter Physics ,Rc columns ,Displacement (vector) ,Seismic analysis ,medicine ,Geotechnical engineering ,medicine.symptom ,Maximum displacement ,business - Abstract
For performance-based seismic engineering of buildings, the direct displacement-based seismic design method is different from the coefficient method used in FEMA-273 and the capacity spectrum method adopted in ATC-40. The method not only is a linear static procedure but also is applied to the design of new constructions. This paper concerns with experimental studies on the accuracy of the direct displacement-based design procedure. Experimental results of three reinforced concrete (RC) columns designed by the displacement procedure are presented and discussed through pseudo-dynamic tests and cyclic loading tests. From the tests, it is shown that the stiffness degrading factor of RC columns plays a key role. The direct displacement-based seismic design method can reliably capture the maximum displacement demand of the test RC columns if the stiffness degrading factor adopted in the displacement design method for RC material is adequate.
- Published
- 2005
32. ZnO Nanowire Device Fabricated By Hydrothermal Synthesis and Dielectrophoresis Method for Gas Sensor Applications
- Author
-
Cheng-Han Lyu, Kai-Heng Sun, Yu-Hung Wang, and Hsun-Feng Hsu
- Abstract
Recently, metal-oxide semiconductor-based gas sensors have gotten a great attention due to their special advantages, such as low cost, high performance and easily fabricated. ZnO has been extensively studied, because it can be applied to sense various gases. One-dimensional ZnO nanostructures can enhance the sensing performance. The vertical nanowire array is a common structure for ZnO nanowire-based gas sensor. However, the seed layer under the nanowire array reduces the sensing capability. Therefore, in this study, the gas sensing devices were fabricated by the arrangement of nanowires between the electrodes. ZnO nanowires were synthesized on the ZnO seed layer by hydrothermal method. The alternating current electrical field was used to align ZnO nanowires of different lengths between the electrodes. For gas sensing application, two types of the nanowire structured devices were used to detect CO. The results show that a flat surface and small grain of seed layer was received by annealing at 300℃, and it can be used to fabricate ZnO nanowires with small fluctuation in length and width by hydrothermal method. ZnO nanowires had the highest aspect ratio when the concentration of precursors was 0.04M. The length of nanowires increased by adding an appropriate concentration of Polyetherimide (PEI). In the process of ZnO nanowires aligment by dielectrophoresis method, the parallel and crossed ZnO nanowire-based devices were achieved by adjusting the frequency and the peak-to-peak voltage of alternating current electrical field. For the CO sensing , the sensing sensitivity and stability of the crossed structure nanowires-based devices were enhanced after annealing. The sensing sensitivity and limit-of-detection of the crossed nanowire-based devices were better than the parallel nanowire-based ones due to the potential barrier modulation at the nanojunctions between two nanowires.
- Published
- 2016
33. ChemInform Abstract: The First Platinum η3-Azatrimethylenemethanes and Platinapyrazolines Formed from Regioselective Addition of Amine or Hydrazine to a σ-Allenyl Complex of Platinum(II)
- Author
-
Yu-Nong Lin, Mou-Chi Cheng, Jie-Shung Chen, Yu-Hung Wang, and Tzu-Yu Huang
- Subjects
chemistry.chemical_compound ,chemistry ,Hydrazine ,Polymer chemistry ,Regioselectivity ,Organic chemistry ,chemistry.chemical_element ,Amine gas treating ,General Medicine ,Platinum - Published
- 2010
34. ChemInform Abstract: Deformation Density Studies of Thiathiophthene. Part 3. Theoretical Studies on 2,5-Dimethylthiathiophthene and 2,4-Diphenylthiathiophthene
- Author
-
Kuan-Jiuh Lin and Yu-Hung Wang
- Subjects
Chemistry ,Chemical physics ,Nanotechnology ,General Medicine ,Deformation (meteorology) - Published
- 2010
35. Structures of 4-acetyl-3-(p-tolyl)sydnone (1) and 4-acetyl-3-phenylsydnone oxime (2)
- Author
-
Chuen-Her Ueng, Mou-Yung Yeh, and Yu-Hung Wang
- Subjects
chemistry.chemical_compound ,Chemistry ,Stereochemistry ,X-ray crystallography ,3-phenylsydnone ,Molecule ,General Medicine ,Crystal structure ,Sydnone ,Oxime ,Medicinal chemistry ,General Biochemistry, Genetics and Molecular Biology - Abstract
Le compose (1) du titre cristallise dans le systeme orthorhombique, groupe d'espace P2 1 2 1 2 1 et sa structure est affinee jusqu'a R=0,038. Le compose (2) cristallise dans le systeme monoclinique, groupe d'espace P2 1 /n avec R=0,041
- Published
- 1987
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.