17 results on '"Yoshifumi Shimomoto"'
Search Results
2. Japanese star anise ringspot-associated virus is a distinct emaravirus transmitted by the eriophyid mite (the family Diptilomiopidae)
- Author
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Tomoyuki Okada, Hironobu Yanagisawa, Kazutaka Yano, Akio Tatara, Kenji Kubota, Yusuke Hasegawa, Kazusa Hayashi, Yoshifumi Shimomoto, Sawana Takeyama, Kenichi Ikeda, and Yasuaki Morita
- Subjects
Sanger sequencing ,biology ,Emaravirus ,RNA ,Illicium anisatum ,Plant Science ,biology.organism_classification ,Virology ,Virus ,symbols.namesake ,Complementary DNA ,symbols ,Movement protein ,Agronomy and Crop Science ,Peptide sequence - Abstract
Japanese star anise (Illicium anisatum L., JSA) is seriously damaged by a ringspot disease in Japan. Herein, to determine the causal agent using high-throughput sequencing, we discovered viral RNAs associated with JSA ringspot disease. We then determined the complete or near-complete nucleotide sequences of these RNAs using Sanger sequencing and RACE. The complementary strand of viral RNAs 1, 2, 3, 4, and 5 encoded a single protein, which shared sequence identity with P1 (RNA-dependent RNA polymerase), P2 (glycoprotein precursor), P3 (nucleocapsid protein), P4 (movement protein), and a protein with unknown function of emaraviruses (genus Emaravirus), respectively; however, the highest amino acid sequence identity for the P1–P5 proteins between JSARaV and known emaraviruses was 41.9%, 30.0%, 30.1%, 52.2%, and 38.0%, respectively, all of which were lower than the species demarcation criterion. Furthermore, RNA segments harbored conserved 12-nt terminal sequences at the 5′- and 3′-termini, and a high complementarity of approximately 20 nt in 5′- and 3′-terminal sequences. Transmission electron microscopy confirmed the presence of virus-like particles. JSA ringspot disease was found to be transmitted by an eriophyid mite (subclass Acari, superfamily Eriophyoidea) that belongs to the family Diptilomiopidae. Taken together, these results identified the virus responsible for the ringspot disease of JSA as a new member of the genus, Emaravirus, which we named as the Japanese star anise ringspot-associated virus (JSARaV). Moreover, this is the first report noting that eriophyid mites of the family Diptilomiopidae are capable of transmitting emaravirus.
- Published
- 2021
3. Complete genome sequence of a novel ophiovirus associated with chlorotic disease of pepper (Capsicum annuum L.) in Japan
- Author
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Yoshifumi Shimomoto, Chika Takemura, Hironobu Yanagisawa, Yutaro Neriya, and Takahide Sasaya
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Virology ,General Medicine - Abstract
In July 2018, pepper plants (Capsicum annuum L.) with chlorotic leaves and fruits were observed in Kochi prefecture, Japan. High-throughput sequencing (HTS) identified the possible presence of an ophiovirus-like virus possessing three RNA segments in a chlorotic leaf. Using Sanger sequencing with primers designed based on the HTS results and a different source of RNA from the one used for HTS, the complete nucleotide sequences of three RNA segments encoding four proteins on their complementary strand were determined. The amino acid sequences of these four proteins showed similarity to those of the RNA-dependent RNA polymerase, RNA-silencing suppressor protein, movement protein, and coat protein, respectively, of ophioviruses, which are negative-sense single-stranded RNA viruses. However, the coat protein amino acid sequence of the virus found on pepper plants was no more than 61.9% identical to those of any known ophioviruses, which is lower than the species demarcation threshold of 85 %. Hence, we suggest that this virus, which we have named "pepper chlorosis associated virus" (PepCaV) should be considered a member of a new species in the genus Ophiovirus, for which we propose the name "Ophiovirus capsici". The results of phylogenetic analysis using coat protein amino acid sequences of PepCaV and other ophioviruses also supported this conclusion. PepCaV RNA was found to have conserved nucleotide sequences at both the 5' and 3' termini of the different RNA segments, and the conserved terminal nucleotide sequences were predicted to form a self-complementary double-stranded region, resulting in a panhandle structure in each of the genomic RNAs.
- Published
- 2022
4. Isolation and characterization of an attenuated strain of an Orthotospovirus, melon yellow spot virus
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Koichi Ishikawa, Kazusa Hayashi, Yasuaki Morita, Tomoka Oki, Shigeharu Takeuchi, Tomoyuki Okada, Yoshifumi Shimomoto, and Kazutaka Yano
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0106 biological sciences ,0301 basic medicine ,chemistry.chemical_classification ,Strain (chemistry) ,Melon ,Inoculation ,food and beverages ,Plant Science ,Biology ,01 natural sciences ,Genome ,Amino acid ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,RNA polymerase ,Glycoprotein ,Agronomy and Crop Science ,Gene ,010606 plant biology & botany - Abstract
An attenuated mutant strain of melon yellow spot virus (MYSV), an Orthotospovirus, designated as SA08-8, was obtained from a pathogenic isolate, C95S, via high temperature treatment, single local lesion isolation, and serial mechanical inoculation. Under greenhouse conditions, the SA08-8 strain conferred the cross-protection against wild-type MYSV but it was not transmissible by its known natural vector melon thrips. In SA08-8 genome, a total of 16 amino acid substitutions were found in the genes encoding RNA-dependent RNA polymerase, glycoprotein precursor, and nonstructural S protein. This attenuated mutant strain is a potential biocontrol agent for MYSV-induced crop diseases in the field.
- Published
- 2021
5. Perilla Mosaic Virus Is a Highly Divergent Emaravirus Transmitted by Shevtchenkella sp. (Acari: Eriophyidae)
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Shigeharu Takeuchi, Tomio Usugi, Yasuhiro Tomitaka, Yuya Chiaki, Kenji Kubota, Fujio Kadono, Yoshifumi Shimomoto, Hironobu Yanagisawa, and Shinya Tsuda
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Genetics ,Perilla frutescens ,biology ,Phylogenetic tree ,Mosaic virus ,Phylogenetics ,Plant virus ,GenBank ,Emaravirus ,Plant Science ,biology.organism_classification ,Agronomy and Crop Science ,Eriophyidae - Abstract
Shiso (Perilla frutescens var. crispa) is widely grown as an important vegetable or herb crop in Japan. Beginning around the year 2000, occurrences of severe mosaic symptoms on shiso were documented and gradually spread across Kochi Prefecture, one of four major shiso production areas in Japan. Next generation sequencing and cloning indicated the presence of a previously unknown virus related to the members of the genus Emaravirus, for which we proposed the name Perilla mosaic virus (PerMV). The genome of PerMV consists of 10 RNA segments, each encoding a single protein in the negative-sense orientation. Of these proteins, P1, P2, P3a, P3b, P4, and P5 show amino acid sequence similarities with those of known emaraviruses, whereas no similarities were found in P6a, P6b, P6c, and P7. Characteristics of the RNA segments as well as phylogenetic analysis of P1 to P4 indicate that PerMV is a distinct and highly divergent emaravirus. Electron microscopy observations and protein analyses corresponded to presence of an emaravirus. Transmission experiments demonstrated that an eriophyid mite, Shevtchenkella sp. (family Eriophyidae), transmits PerMV with a minimum 30-min acquisition access period. Only plants belonging to the genus Perilla tested positive for PerMV, and the plant−virus−vector interactions were evaluated. The nucleotide sequences reported here are available in the DDBJ/ENA/GenBank databases under accession numbers LC496090 to LC496099.
- Published
- 2020
6. First report of ‘Candidatus Phytoplasma solani’ associated with pepper chlorosis of sweet pepper, Capsicum annuum L., in Japan
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Shigeharu Takeuchi, Kenichi Ikeda, Yasushi Asahina, Kazutaka Yano, Yasuaki Morita, Yoshifumi Shimomoto, Junki Yamasaki, Misako Oka, and Tomoka Oki
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0106 biological sciences ,0301 basic medicine ,Chlorosis ,biology ,food and beverages ,Plant Science ,biology.organism_classification ,16S ribosomal RNA ,01 natural sciences ,03 medical and health sciences ,Capsicum annuum ,Horticulture ,030104 developmental biology ,Phytoplasma ,Pepper ,Candidatus Phytoplasma solani ,Candidatus Phytoplasma ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
In June 2017, sweet pepper plants showing chlorosis in leaves and fruits were observed in Susaki City, Kochi Prefecture, Japan. We had named the disease pepper chlorosis (Tairyoku-byo). A phytoplasma was observed by electron microscopy and identified as ‘Candidatus Phytoplasma solani’, based on homology searches and molecular phylogeny using nucleotide sequences of 16S rDNA. To our knowledge, this is the first report showing the occurrence of ‘Ca. P. solani’ in Japan.
- Published
- 2019
7. Perilla Mosaic Virus Is a Highly Divergent Emaravirus Transmitted by
- Author
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Kenji, Kubota, Tomio, Usugi, Yasuhiro, Tomitaka, Yoshifumi, Shimomoto, Shigeharu, Takeuchi, Fujio, Kadono, Hironobu, Yanagisawa, Yuya, Chiaki, and Shinya, Tsuda
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Japan ,Mosaic Viruses ,Animals ,Perilla ,Phylogeny ,Plant Diseases - Abstract
Shiso (
- Published
- 2020
8. Problem and future prospect of integrated pest management in Kochi Prefecture
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Yoshifumi Shimomoto, Kazuhide Nakaishi, and Wakana Tanaka
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Integrated pest management ,Business ,Environmental planning - Published
- 2018
9. Multiplex polymerase chain reaction discriminates which eggplant isolates of Corynespora cassiicola are virulent to sweet pepper
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Akinori Kiba, Yoshifumi Shimomoto, and Yasufumi Hikichi
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biology ,fungi ,food and beverages ,Virulence ,Plant Science ,biology.organism_classification ,Corynespora ,law.invention ,Microbiology ,law ,Pepper ,Multiplex polymerase chain reaction ,Blight ,Primer (molecular biology) ,Corynespora cassiicola ,Agronomy and Crop Science ,Polymerase chain reaction - Abstract
Some isolates of Corynespora cassiicola from eggplant leaves with black blight can also cause Corynespora blight on sweet pepper. To differentiate eggplant isolates differing in virulence to sweet pepper, we first created two sets of primers based on nucleotide sequences of DNA fragments specific to virulent and to avirulent isolates, respectively, in a random amplified polymorphic DNA analysis using two random primers. A multiplex polymerase chain reaction using the two primer sets allowed us to discriminate isolates virulent to sweet pepper from among eggplant isolates.
- Published
- 2015
10. Bacterial leaf blight of sweet pepper (Capsicum annuum) caused by Pseudomonas cichorii in Japan
- Author
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Shigeharu Takeuchi, Rie Gappa-Adachi, Yasuaki Morita, and Yoshifumi Shimomoto
- Subjects
Sweet Peppers ,biology ,Spots ,fungi ,food and beverages ,Plant Science ,biology.organism_classification ,16S ribosomal RNA ,Capsicum annuum ,Pepper ,Botany ,Blight ,Agronomy and Crop Science ,Bacteria ,Pseudomonas cichorii - Abstract
Sweet peppers (cv. Tosajishi beauty) with leaf blight symptoms were observed in Kochi Prefecture, 2003. Initially, small spots formed on leaves, and later enlarged to brown to dark brown spots, and eventually blighted leaves fell. Several bacteria that caused the same symptoms were isolated and subsequently reisolated. These bacteria were identified as Pseudomonas cichorii on the basis of bacterial characteristics and the 16S rRNA gene sequence. This is the first report of bacterial leaf blight in the sweet pepper in the world.
- Published
- 2013
11. Amino acids in Tobamovirus coat protein controlling pepper L1a gene-mediated resistance
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Yoshifumi Shimomoto, Masamichi Nishiguchi, Hiroyuki Mizumoto, Ken-Taro Sekine, Akinori Kiba, Reiko Tomita, Hiromasa Sawada, Yasufumi Hikichi, Ikumi Nakamura, and Kappei Kobayashi
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chemistry.chemical_classification ,Pepper mild mottle virus ,biology ,Strain (chemistry) ,Inoculation ,Soil Science ,Tobamovirus ,Plant Science ,biology.organism_classification ,Virology ,Molecular biology ,Amino acid ,Elicitor ,chemistry ,Pepper ,Agronomy and Crop Science ,Molecular Biology ,Gene - Abstract
In pepper plants (genus Capsicum), the resistance against Tobamovirus spp. is conferred by L gene alleles. The recently identified L variant L(1a) can recognize coat proteins (CPs) of Tobacco mild green mosaic virus Japanese strain (TMGMV-J) and Paprika mild mottle virus Japanese strain (PaMMV-J), but not of Pepper mild mottle virus (PMMoV), as the elicitor to induce resistance at 24 °C. Interestingly, L(1a) gene-mediated resistance against TMGMV-J, but not PaMMV-J, is retained at 30 °C. This observation led us to speculate that L(1a) can discriminate between CPs of TMGMV-J and PaMMV-J. In this study, we aimed to determine the region(s) in CP by which L(1a) distinguishes TMGMV-J from PaMMV-J. By using chimeric CPs consisting of TMGMV-J and PaMMV-J, we found that the chimeric TMGMV-J CP, whose residues in the β-sheet domain were replaced by those of PaMMV-J, lost its ability to induce L(1a) gene-mediated resistance at 30 °C. In contrast, the chimeric PaMMV-J CP with the β-sheet domain replaced by TMGMV-J CP was able to induce L(1a) gene-mediated resistance at 30 °C. Furthermore, viral particles were not detected in the leaves inoculated with either chimeric virus. These observations indicated that the amino acids within the β-sheet domain were involved in both the induction of L(1a) gene-mediated resistance and virion formation. Further analyses using chimeric CPs of TMGMV-J and PMMoV indicated that amino acids within the β-sheet domain alone were not sufficient for the induction of L(1a) gene-mediated resistance by TMGMV-J CP. These results suggest that multiple regions in Tobamovirus CP are implicated in the induction of L(1a) gene-mediated resistance.
- Published
- 2012
12. Breeding of mother lines of Capsicum resistant to Corynespora blight caused by Corynespora cassiicola
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Yasufumi Hikichi, M. Okada, Hiroyuki Sawada, Akinori Kiba, Y. Morita, Yoshifumi Shimomoto, and Hiroyuki Mizumoto
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Horticulture ,Blight ,Biology ,Corynespora cassiicola ,biology.organism_classification ,Corynespora - Published
- 2012
13. Reidentification of the causal fungus of black blight of eggplant
- Author
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Y. Morita, Akinori Kiba, T. Sato, Yoshifumi Shimomoto, Yasufumi Hikichi, and Shigeharu Takeuchi
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Melongena ,biology ,fungi ,Botany ,food and beverages ,Blight ,Fungus ,Solanum ,biology.organism_classification ,Pathogenicity ,Pathogen ,Corynespora - Abstract
The pathogen of black blight of eggplant (Solanum melongena L.) was previously described as Corynespora melongenae Takimoto in Japan, but there was no Latin description of the fungus. We collected the fungus again in Shimane and Kochi prefectures, the western regions of Japan and reidentified them as C. cassiicola based on the morphological characteristics and sequences of rDNA-ITS regions after confirming their pathogenicity on eggplant. We propose to replace C. melongenae Takimoto nom. inval. with C. cassiicola as the pathogen of black blight in eggplant in Japan.
- Published
- 2011
14. Pathogenic and genetic variation among isolates of Corynespora cassiicola in Japan
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Yasufumi Hikichi, Y. Morita, Yoshifumi Shimomoto, Akinori Kiba, S. Takeuchi, T. Sato, H. Hojo, and Hiroyuki Mizumoto
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Veterinary medicine ,biology ,fungi ,food and beverages ,Virulence ,Plant Science ,Fungi imperfecti ,Horticulture ,biology.organism_classification ,Perilla ,RAPD ,Genetic variation ,Botany ,Pepper ,Genetics ,Genetic variability ,Corynespora cassiicola ,Agronomy and Crop Science - Abstract
In order to develop a method for discrimination of Corynespora cassiicola isolates pathogenic to sweet pepper among Japanese isolates, this study analysed pathogenic variations of 64 Japanese isolates of C. cassiicola on perilla, cucumber, tomato, aubergine and sweet pepper, and their multigene phylogeny. Japanese isolates were divided into seven pathogenicity groups (PG1–PG7). The virulence of isolates in PG1–PG5 was restricted to perilla, cucumber, tomato, aubergine and sweet pepper, respectively. Isolates in PG6 were virulent to sweet pepper, tomato and aubergine. Isolates in PG7 were avirulent to all tested plants. Multigene phylogenetic analysis of the isolates based on β-tubulin, translation elongation factor 1-α, calmodulin and actin genes showed three divergent clusters, MP-A, MP-B and MP-C. These clusters included all isolates in PG1, PG2, PG8 and PG9 (MP-A), PG3 and PG5 (MP-B) and PG4 and PG6 (MP-C). Isolates in PG7 were distributed amongst all clusters. Furthermore, random amplified polymorphic DNA (RAPD) analysis using universal primers, Q17 (5′-GAAGCCCTTG-3′) and Q13 (5′-GGAGTGGACA-3′), facilitated discrimination of isolates virulent on sweet pepper amongst isolates in MP-B and MP-C, respectively. Together, a combination of the multigene analysis and the RAPD technique allowed the discrimination of the isolates virulent to sweet pepper.
- Published
- 2010
15. First report of Melon yellow spot virus infecting balsam pear (Momordica charantia L.) in Japan
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Koichi Ishikawa, Yoshifumi Shimomoto, and Shigeharu Takeuchi
- Subjects
Balsam ,PEAR ,Momordica ,biology ,Spots ,Plant Science ,biology.organism_classification ,Virology ,Virus ,Serology ,body regions ,Horticulture ,Plant virus ,Agronomy and Crop Science ,Pathogen - Abstract
A new disease causing necrotic spots and yellowing on leaves of balsam pear (Momordica charantia) was found in Kochi Prefecture, Japan. In this study, we identified the causal pathogen as Melon yellow spot virus (MYSV) based on morphology of virus particles, serology, and the nucleotide sequence of the nucleocapsid protein gene. This is the first report of natural infection of balsam pear by MYSV. We propose the name spotted wilt for this new disease of balsam pear.
- Published
- 2009
16. Corynespora blight of sweet pepper (Capsicum annuum) caused by Corynespora cassiicola (Berk. & Curt.) Wei
- Author
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Yoshifumi Shimomoto, Rie Adachi, Yasuaki Morita, Yasufumi Hikichi, Kazutaka Yano, Akinori Kiba, and Shigeharu Takeuchi
- Subjects
Spots ,digestive, oral, and skin physiology ,fungi ,food and beverages ,Plant Science ,Fungus ,Biology ,Pathogenicity ,biology.organism_classification ,Corynespora ,Capsicum annuum ,stomatognathic system ,Pepper ,Botany ,Blight ,lipids (amino acids, peptides, and proteins) ,Corynespora cassiicola ,Agronomy and Crop Science - Abstract
In 2004, Corynesopra cassiicola was isolated from dark brown spots on leaves and fruits and from black blights on stems of sweet pepper plants in Kochi Prefecture, Japan. The isolated fungus was then used to inoculate sweet pepper plants and subsequently reisolated from the plants with dark brown spots and black blights, showing that C. cassiicola is a new pathogen causing Corynespora blight on sweet pepper plants.
- Published
- 2008
17. Amino acids in Tobamovirus coat protein controlling pepper L(1a) gene-mediated resistance
- Author
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Hiroyuki, Mizumoto, Ikumi, Nakamura, Yoshifumi, Shimomoto, Hiromasa, Sawada, Reiko, Tomita, Ken-Taro, Sekine, Akinori, Kiba, Masamichi, Nishiguchi, Kappei, Kobayashi, and Yasufumi, Hikichi
- Subjects
Sequence Homology, Amino Acid ,Molecular Sequence Data ,Tobamovirus ,Virion ,Capsid Proteins ,Amino Acid Sequence ,Original Articles ,Capsicum ,Genes, Plant - Abstract
In pepper plants (genus Capsicum), the resistance against Tobamovirus spp. is conferred by L gene alleles. The recently identified L variant L(1a) can recognize coat proteins (CPs) of Tobacco mild green mosaic virus Japanese strain (TMGMV‐J) and Paprika mild mottle virus Japanese strain (PaMMV‐J), but not of Pepper mild mottle virus (PMMoV), as the elicitor to induce resistance at 24 °C. Interestingly, L(1a) gene‐mediated resistance against TMGMV‐J, but not PaMMV‐J, is retained at 30 °C. This observation led us to speculate that L(1a) can discriminate between CPs of TMGMV‐J and PaMMV‐J. In this study, we aimed to determine the region(s) in CP by which L(1a) distinguishes TMGMV‐J from PaMMV‐J. By using chimeric CPs consisting of TMGMV‐J and PaMMV‐J, we found that the chimeric TMGMV‐J CP, whose residues in the β‐sheet domain were replaced by those of PaMMV‐J, lost its ability to induce L(1a) gene‐mediated resistance at 30 °C. In contrast, the chimeric PaMMV‐J CP with the β‐sheet domain replaced by TMGMV‐J CP was able to induce L(1a) gene‐mediated resistance at 30 °C. Furthermore, viral particles were not detected in the leaves inoculated with either chimeric virus. These observations indicated that the amino acids within the β‐sheet domain were involved in both the induction of L(1a) gene‐mediated resistance and virion formation. Further analyses using chimeric CPs of TMGMV‐J and PMMoV indicated that amino acids within the β‐sheet domain alone were not sufficient for the induction of L(1a) gene‐mediated resistance by TMGMV‐J CP. These results suggest that multiple regions in Tobamovirus CP are implicated in the induction of L(1a) gene‐mediated resistance.
- Published
- 2012
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