9 results on '"Ying-yin LIANG"'
Search Results
2. Isolation, characterization, and genome sequencing of a novel chitin deacetylase producing
- Author
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Ying-Yin, Liang, Lu-Qi, Yan, Ming-Hui, Tan, Gang-Hui, Li, Jian-Hao, Fang, Jie-Ying, Peng, and Kun-Tai, Li
- Abstract
Chitin deacetylase (CDA) is a chitin degradation enzyme that catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues, playing an important role in the high-value utilization of waste chitin. The shells of shrimp and crab are rich in chitin, and mangroves are usually recognized as an active habitat to shrimp and crab. In the present study, a CDA-producing bacterium, strain TCI-16, was isolated and screened from the mangrove soil. Strain TCI-16 was identified and named as
- Published
- 2022
3. Diffusion-Tensor Imaging of Thigh Muscles in Duchenne Muscular Dystrophy: Correlation of Apparent Diffusion Coefficient and Fractional Anisotropy Values With Fatty Infiltration
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Ying Yin Liang, Gui Dian Li, Ping Xu, Ying Ming Chen, and Jian Ling
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Male ,musculoskeletal diseases ,Adolescent ,Duchenne muscular dystrophy ,Rectus femoris muscle ,Thigh ,030218 nuclear medicine & medical imaging ,Correlation ,03 medical and health sciences ,0302 clinical medicine ,Fractional anisotropy ,medicine ,Humans ,Effective diffusion coefficient ,Radiology, Nuclear Medicine and imaging ,Child ,Muscle, Skeletal ,business.industry ,Thigh muscle ,Infant ,General Medicine ,Anatomy ,musculoskeletal system ,medicine.disease ,Muscular Dystrophy, Duchenne ,body regions ,Diffusion Tensor Imaging ,medicine.anatomical_structure ,Adipose Tissue ,Case-Control Studies ,Child, Preschool ,Anisotropy ,business ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
The purpose of this study is to investigate the correlation of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) values with fatty infiltration in the thigh muscles of patients with Duchenne muscular dystrophy (DMD) using diffusion-tensor imaging (DTI).Twenty-one boys with DMD were recruited. The grade of fatty infiltration and the ADC and FA values of four thigh muscles (rectus femoris, semitendinosus, sartorius, and gracilis) were measured, and the FA and ADC values were compared with the grade of fatty infiltration. Twenty age-matched healthy boys were enrolled as the control group. The differences in the ADC and FA values of the thigh muscles between patients with DMD and the control group were compared.The patients with DMD showed lower FA values and higher ADC values in all measured muscles when compared with the control group. The FA and ADC values were correlated with the grade of fatty infiltration. For the rectus femoris muscle, r = -0.753 and p = 0.007 for FA, and r = 0.685 and p = 0.001 for ADC. For the semitendinosus muscle, r = -0.621 and p = 0.041 for FA, and r = 0.705 and p = 0.021 for ADC. For the sartorius muscle, r = -0.662 and p = 0.027 for FA, and r = 0.701 and p = 0.017 for ADC. For the gracilis muscle, r = -0.618 and p = 0.043 for FA, and r = 0.695 and p = 0.022 for ADC.Damage to the thigh muscles in patients with DMD can be detected by ADC and FA values using DTI. DTI can be used to assess the severity of the disease.
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- 2016
4. Serum Creatinine Level: A Supplemental Index to Distinguish Duchenne Muscular Dystrophy from Becker Muscular Dystrophy
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Ying-yin Liang, Xingxuan Wen, Yu Zhang, Cheng Zhang, Hui-li Zhang, Yuling Zhu, Ya-qin Li, Yiming Sun, and Langhui Deng
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Male ,musculoskeletal diseases ,medicine.medical_specialty ,Article Subject ,Adolescent ,Duchenne muscular dystrophy ,Clinical Biochemistry ,Gastroenterology ,chemistry.chemical_compound ,Disease severity ,Internal medicine ,Partial correlation analysis ,Genetics ,medicine ,Humans ,In patient ,Muscular dystrophy ,Child ,Inverse correlation ,Molecular Biology ,lcsh:R5-920 ,Creatinine ,business.industry ,Biochemistry (medical) ,General Medicine ,medicine.disease ,Muscular Dystrophy, Duchenne ,Endocrinology ,chemistry ,Child, Preschool ,lcsh:Medicine (General) ,business ,Biomarkers ,Serum creatinine level ,Research Article - Abstract
Background.To improve assessment of dystrophinopathy, the aim of this study was to identify whether serum creatinine (Crn) level reflects disease severity.Methods.Biochemical, Vignos score, and genetic data were collected on 212 boys with dystrophinopathy.Results.Serum Crn level had a strong inverse correlation with Vignos score by simple correlation(r=-0.793)and partial correlation analysis after adjustment for age, height, and weight (r=-0.791; bothP<0.01). Serum Crn level was significantly higher in patients with in-frame than out-of-frame mutations(Z=-4.716, P<0.01)and in Becker muscular dystrophy (BMD) patients than Duchenne muscular dystrophy (DMD) patients at ages 4, 5, 7, and 9 yr (allP<0.0125). After adjusting for age, height, and weight, BMD patients still had a significantly higher serum Crn level than DMD patients(β=7.140, t=6.277, P<0.01).Conclusions.Serum Crn level reflected disease severity and may serve as a supplemental index to distinguish DMD from BMD in clinical practice.
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- 2015
5. Adipose-derived stem cells enhance myogenic differentiation in the mdx mouse model of muscular dystrophy
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Ji-Qing, Cao, Ying-Yin, Liang, Ya-Qin, Li, Hui-Li, Zhang, Yu-Ling, Zhu, Jia, Geng, Li-Qing, Yang, Shan-Wei, Feng, Juan, Yang, Jie, Kong, and Cheng, Zhang
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Duchenne muscular dystrophy ,myogenic differentiation ,paracrine pathway ,adipose-derived stem cells ,nerve regeneration ,neural regeneration ,dystrophin ,Research Article - Abstract
Adipose-derived stem cells have been shown to promote peripheral nerve regeneration through the paracrine secretion of neurotrophic factors. However, it is unclear whether these cells can promote myogenic differentiation in muscular dystrophy. Adipose-derived stem cells (6 × 106) were injected into the gastrocnemius muscle of mdx mice at various sites. Dystrophin expression was found in the muscle fibers. Phosphorylation levels of Akt, mammalian target of rapamycin (mTOR), eIF-4E binding protein 1 and S6 kinase 1 were increased, and the Akt/mTOR pathway was activated. Simultaneously, myogenin levels were increased, whereas cleaved caspase 3 and vimentin levels were decreased. Necrosis and fibrosis were reduced in the muscle fibers. These findings suggest that adipose-derived stem cells promote the regeneration and survival of muscle cells by inhibiting apoptosis and fibrosis, thereby alleviating muscle damage in muscular dystrophy.
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- 2016
6. [Study on Duchenne muscular dystrophy gene mutation and prenatal diagnosis]
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Shan-wei, Feng, Ying-yin, Liang, Ji-qing, Cao, Xin-ming, Song, and Cheng, Zhang
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Dystrophin ,Male ,Muscular Dystrophy, Duchenne ,China ,Asian People ,Pregnancy ,Prenatal Diagnosis ,Mutation ,Humans ,Female ,Exons - Abstract
To explore the characteristics of DNA mutations underlying Duchenne muscular dystrophy and provide prenatal diagnosis.Multiplex ligation-dependent probe amplification (MLPA) and denaturing high performance liquid chromatography (DHPLC) were applied for analyzing DMD gene mutations in 388 unrelated Chinese patients and 53 fetuses.Respectively, 230 and 43 subjects were found to harbor a deletion (59.28%) or duplication (11.08%). Two deletion hotspots were identified, which have located at exons 45-54 and exons 3-19. Duplications were mainly detected at exons 2-43. Point mutations were identified in 29.64% of patients. Fifty three fetuses were prenatal diagnosed, among which 18 were identified as patients.Frequencies of DMD gene deletions and duplications in China are similar to global data. Prenatal diagnosis can help to reduce births of DMD patients.
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- 2013
7. [Correlation between genotypes and phenotypes in pseudohypertrophic muscular dystrophy]
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Shan-wei, Feng, Ying-yin, Liang, Ji-qing, Cao, Xin-ming, Song, and Cheng, Zhang
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Dystrophin ,Muscular Dystrophy, Duchenne ,Phenotype ,Genotype ,Mutation ,Humans ,Exons ,Genetic Association Studies - Abstract
To explore the correlation between genotypes and phenotypes in Chinese patients with pseudohypertrophic muscular dystrophy.Patients with Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) were diagnosed clinically. Multiplex ligation-dependent probe amplification (MLPA) were performed to detect potential DMD gene mutations. The results were analyzed statistically.Among 280 patients, 238(85.0%) were diagnosed with DMD, 35(12.50%) were diagnosed with BMD and 7(2.5%) were diagnosed with intermediate muscular dystrophin (IMD). Among these, 252(92.31%) were in-frame mutations, and 21(7.69%) were out-of-frame mutations. Twelve patients with DMD have carried in-frame mutations, 9 with BMD have carried frame-shift mutations, and 7 IMD patients have carried frame-shift mutation.Most of the genotypes and phenotypes of DMD have complied with the reading-frame hypothesis. Patients with BMD with frame-shift mutations may facilitate understanding of the pathogenesis of DMD, and provide a theoretical basis for clinical therapy.
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- 2012
8. [Values of serum copper and serum free copper in the diagnosis and monitoring of Wilson's disease and its carriers]
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Xiang-xue, Zhou, Xun-hua, Li, Hai-wei, Huang, Bing, Liu, Rong-lan, Zhu, Ying-yin, Liang, Jian-zhong, Zhu, and Xiu-ling, Liang
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Adult ,Male ,Heterozygote ,Young Adult ,Adolescent ,Hepatolenticular Degeneration ,Case-Control Studies ,Humans ,Female ,Copper - Abstract
To explore the values of serum copper and serum free copper in the diagnosis of Wilson's disease (WD), its carrier and viral hepatitis and explore the guiding significance of monitoring serum copper in the treatment of WD.A total of 80 WD patients (hepatic type, n = 60; encephalic type, n = 20), 30 carriers, 20 patients with viral hepatitis were enrolled and their levels of serum copper were determined. The neural symptoms were scored by modified Young grade. Hemogram, hepatic functions, blood clotting functions, serum copper and urinary copper were tested throughout all 8 courses of treatment with sodium dimercaptopropane sulfonate (DMPS). The patients were treated with zinc after discharging. All data were analyzed.The free serum copper increased in the patients with WD (0.17 mg/L ± 0.04 mg/L), carriers (0.13 mg/L ± 0.03 mg/L) and severe viral hepatitis (0.12 mg/L). A slight increase was also observed in the WD carriers. The level of serum copper was correlated with hepatic functions but not with the severity of neural symptoms. The serum copper increased in the patients with no improvement of neural symptoms. However, the serum copper decreased in the WD patients with the improvement of neural symptoms. The serum copper was stabilized at approximately 0.2 mg/L during the long-term treatment period.There is auxiliary diagnosis significance of serum copper in the determination of WD. Hepatic functions in hepatic type WD affect the level of serum copper. The serum copper of encephalic type WD can not indicate the severity of neural symptoms. The elevated level of serum copper indicates a poor prognosis. The serum copper is an effective marker in monitoring the development and therapeutic efficacy of the disease.
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- 2012
9. [Preliminary study of the spatial structural and functional changes of dystrophin after exon-3 deletion]
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Ying-Yin, Liang, Cheng, Zhang, Song-Lin, Chen, and Shan-Wei, Feng
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Dystrophin ,Models, Molecular ,Muscular Dystrophy, Duchenne ,Structure-Activity Relationship ,Protein Conformation ,Humans ,Exons ,Protein Binding ,Protein Structure, Tertiary ,Sequence Deletion - Abstract
To explore the structural and functional changes of dystrophin molecule after exon 3 deletion.Three-dimensional models of dystrophin comprising the major domains were established before and after exon 3 deletion using SWISS-MODEL server. The motifs and structural domains of dystrophin after exon 3 deletion were searched in Pfam database, and the crystal structure of the actin-binding domain in the dystrophin molecule was analyzed using Rasmol software.Torsion of the N-terminal actin-binding domain occurred in the dystrophin molecule after deletion of exon 3. Homology analysis based on Pfam database searches indicated that following exon 3 deletion, the Bit score of the first calponin homology (CH1) domain was decreased from 108 to 36.5 while its expectation value increased from 2.3e-9 to 8.1e-8. The deletion also resulted in the absence of the spiral region C from the CH1 domain.Exon 3 deletion in the dystrophin-coding sequence decreases the stability of CH1 domain and prevents the formation of the junction interface where dystrophin binds to actin. The bioinformatics approach provides a new alternative for investigation of the pathogenesis of DMD pathogenesy investigation.
- Published
- 2008
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