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Helicoverpa zea (Boddie) is a destructive agricultural pest species that is targeted by both Bacillus thuringiensis (Bt) maize and cotton in the United States. Cry1A.105 and Cry2Ab2 are two Bt proteins expressed in a widely planted maize event MON 89034. In this study, two tests (Test-I and Test-II) were conducted to evaluate the relative fitness of Bt-susceptible and -resistant H. zea on non-Bt diet (Test-I and Test-II) and a diet containing a mix of Cry1A.105 and Cry2Ab2 at a low concentration (Test-II only). Insect populations evaluated in Test-I were two Bt-susceptible strains and three Bt-resistant strains (a single-protein Cry1A.105-, a single-protein Cry2Ab2-, and a dual-protein Cry1A.105/Cry2Ab2-resistant strains). Test-II analyzed the same two susceptible strains, three backcrossed-and-reselected Cry1A.105/Cry2Ab2-single-/dual-protein-resistant strains, and three F

Objectives. This meta-analysis was to verify the short-time efficacy and safety of abciximab in patients with ST-segment elevation myocardial infarction (STEMI) undergoing percutaneous coronary intervention (PCI). Background. Abciximab has long-term efficacy in patients with STEMI undergoing PCI, but the short-term efficacy is still controversial. Methods. We conducted a systematic review and meta-analysis compared with or without abciximab in patients with STEMI undergoing PCI. The relevant randomized controlled trials were included by searching PubMed, EMBASE, Cochrane Library, and Web of Science databases and other sources. The relative risk (RR) and 95% confidence intervals (CI) of outcomes were calculated by the fixed-effects model. Results. Ten randomized controlled trials with 5008 patients met inclusion criteria. There were no significant differences in risk of all-cause death at 30-day (RR 0.79, CI 0.55–1.12, P = 0.18 ), major bleeding (1.37, 0.93–2.03, P = 0.11 ), and transfusion (1.23, 0.94–1.61, P = 0.13 ) between the two groups. However, there were significant differences in risk of all-cause death at 6 months (0.57, 0.36–0.90, P = 0.02 ), recurrent myocardial infarction (0.55, 0.33–0.92, P = 0.02 ), repeat revascularization (0.58, 0.43–0.78, P = 0.0004 ), final TIMI flow P = 0.02 ), minor bleeding (1.29, 1.02–1.63, P = 0.04 ), and thrombocytopenia (2.04, 1.40–2.97, P = 0.0002 ). Conclusions. The application of abciximab can lead to a lower risk of reinfarction, revascularization, and all-cause death at 6 months, but a higher risk of minor bleeding, and thrombocytopenia.

The purpose of this meta-analysis was to evaluate the efficacy and safety of proton pump inhibitors (PPIs) plus antithrombotic strategy in patients with coronary artery diseases compared with antithrombotic strategy alone. We searched PubMed, EMBASE, Cochrane Library, and Chinese Biomedical Medical Literature databases to retrieve randomized controlled trials investigating PPIs combined with antithrombotic strategy in coronary artery diseases. The primary efficacy outcome was major adverse cardiovascular and cerebrovascular events (MACCE). The primary safety outcome was gastrointestinal events. Secondary outcomes included all-cause death, cardiovascular death, myocardial infarction, stent thrombosis, significant bleeding from gastroduodenal lesions, and gastroduodenal ulcer. Overall, 43,943 patients were enrolled from 19 trials. The incidence of MACCE [relative risk (RR) 1.05; 95% confidence interval (CI) 0.96-1.15], all-cause death (RR 0.84; 95% CI 0.69-1.01), cardiovascular death (RR 0.88; 95% CI 0.69-1.12), myocardial infarction (RR 0.98; 95% CI 0.88-1.09), stent thrombosis (RR 1.01; 95% CI 0.76-1.34), and gastroduodenal ulcer (RR 0.40; 95% CI 0.13-1.29) did not increase significantly in patients receiving PPIs compared with patients without those. There were significant differences in the risk of gastrointestinal events (RR 0.34; 95% CI 0.21-0.54) and significant bleeding from gastroduodenal lesions (RR 0.09; 95% CI 0.03-0.28) between the 2 groups. In patients with coronary artery diseases, PPIs plus antithrombotic strategy could reduce the risk of gastrointestinal events and significant bleeding from gastroduodenal lesions but may not affect the incidence of MACCE, all-cause death, cardiovascular death, myocardial infarction, stent thrombosis, and gastroduodenal ulcer (PROSPERO: CRD42021277899, date of registration October 10, 2021).

Though various therapeutic strategies have been developed to overcome gastric cancer, the overall prognosis and therapeutic effect are still not optimistic. As a novel identified type of cell death, ferroptosis has been considered as a promising tumor suppression mechanism with therapeutic potential against gastric cancer. In this work, we screened a collection of 4890 bioactivity compounds and committed to find novel agents that can induce apoptosis in gastric cancer. Among these compounds, 6-TG was identified as a potential ferroptosis inducer in gastric cancer cells for the first time. It could inactivate system xc

Sever plastic deformation (SPD) with high strain rate can increase the material dislocation density, reduce the grain size and improve the mechanical properties. In this paper, ultrasonic compound cutting (UCC) was proposed to improve the efficiency of preparing ultra-fine grained (UFG) pure copper by sever plastic deformation methods. The motion characteristics and strain rate model of UCC were analyzed, and it was concluded that ultrasonic vibration can increase the strain rate in the primary shear zone. According to the 3D FEM equivalent model of UCC, the UCC and traditional compound cutting (TCC) were compared and analyzed from the perspective of strain rate. The simulation results show that ultrasonic vibration can significantly increase the strain rate of chip. The microstructure and mechanical properties of pure copper chip were studied by using a self-developed machining device. The experiment results show that the grain refinement, dislocation density and micro-hardness of pure copper chip were significantly improved in UCC. When the ultrasonic amplitude is 3 µm, the UCC chip grains are about 2.66 µm and the hardness reaches 124 HV, which is about 8% higher than the hardness of TCC chip. The findings of this research provide an important reference for machining UFG pure copper with enhanced mechanical properties.

Parkinson's disease (PD) represents the second most common neurodegenerative disease.To evaluate the effects of dance therapy (DT) aimed at improving non-motor symptoms in PD.Studies were performed through PubMed, Web of Science, The Cochrane Library, Embase, and Science Direct from inception to October 27, 2021. The data were screened independently by two reviewers, and the quality of the papers was assessed using the Cochrane manual. The included studies were randomized controlled trials and quasi-randomized controlled trials, reporting random-effects standardized mean differences, and 95% confidence intervals as the effect size. INine literatures were analyzed for the meta-analysis with a total of 307 patients. Random effects showed that DT significantly improved cognitive of PD (MD = 1.50, 95% CI [0.52, 2.48], P = 0.0003; IThe meta-analysis suggests that dance can improve cognitive function in PD.

Dehydrogenative coupling of arenes with fluoroaryl nucleophiles enables the efficient synthesis of fluoroaromatic compounds from readily available starting materials.

The salp swarm algorithm (SSA) will converge prematurely and fall into local optimum when solving complex high-dimensional multimodal optimization tasks. This paper proposes an improved SSA (GMLSSA) based on gravitational search and multi-swarm search strategies. In the gravitational search strategy, using multiple salp individuals to guide the location update of search agents can get rid of the limitation of individual guidance and improve the exploration ability of the algorithm. In the multi-swarm leader strategy, the original population is divided into several independent subgroups to increase population diversity and avoid falling into local optimization. In the experiment, 20 benchmark functions (including the well-known CEC 2014 function) were used to test the performance of the proposed GMLSSA in different dimensions, and the results were compared with the most advanced search algorithm and SSA variants. The experimental results are evaluated through four different analysis methods: numerical, stability, high-dimensional performance, and statistics. These results conclude that GMLSSA has better solution quality, convergence accuracy, and stability. In addition, GMLSSA is used to solve the tension/compression spring design problem (TCSD). The proposed GMLSSA is superior to other competitors in terms of solution quality, convergence accuracy, and stability.

High-quality and highly precision dressing is essential to achieving precision form grinding of GCr15 bearing rings. A method of longitudinal-torsional ultrasonic assisted roller dressing (LTUARD) was proposed for achieving high precision and high-quality dressing. A device of LTUARD was developed for investigating the dressing effect of the longitudinal-torsional ultrasonic assisted roller on the white alumina (WA) grinding wheel. The longitudinal-torsional horn with roller loading was mainly studied during the device development of LTUARD. The geometric dimensions of the longitudinal vibration horn with large tool head were firstly determined by the set of transcendental equations, and then the longitudinal vibration was converted to longitudinal torsional vibration by adding a helical slot in the conical section. Subsequently, the results of the impedance analysis and vibration characteristics experiments of the longitudinal-torsional ultrasonic vibration system show that the vibration system meets the vibration requirements of LTUARD. The vitrified bonded white alumina (WA) grinding wheel dressing experiments were carried out using conventional roller dressing (CRD) and LTUARD respectively, and the grinding of GCr15 bearing rings with the before dressing (BD) and after dressing of WA grinding wheel. The results of dressing and grinding experiments show that the surface morphology and roundness error of WA grinding wheel are improved after LTUARD, and the surface quality of GCr15 bearing rings ground by WA grinding wheel after LTUARD dressing is better. The reliability and practicality of the developed dressing device were verified by the dressing and grinding experiment results.

The optimal duration of dual antiplatelet therapy (DAPT) for patients implanted with new-generation drug-eluting stents in East Asians is currently still controversial. The purpose of this meta-analysis was to investigate the efficacy and safety of short-term DAPT in patients with those. In this study, randomized controlled trials from PubMed, EMBASE, and Cochrane Library were searched to compare the efficacy and safety of short-term DAPT (6 months or less) with long-term DAPT (12 months or more) in patients implanted with new-generation drug-eluting stents in East Asian from inception to September 2020. The primary efficacy outcome was all-cause death, the primary safety outcome was major bleeding, and the secondary outcomes included cardiovascular death, myocardial infarction, definite or possible stent thrombosis, and stroke. A total of 6 randomized controlled trials with 15,688 patients met inclusion criteria; there were no significant differences in the incidence of all-cause death [risk ratio (RR), 1.03; 0.76-1.39; P = 0.856)], cardiovascular death (RR, 0.83; 0.55-1.24; P = 0.361), myocardial infarction (RR, 0.97; 0.72-1.31; P = 0.853), definite or possible stent thrombosis (RR, 1.52; 0.83-2.78; P = 0.170), and stroke (RR, 0.90; 0.61-1.31; P = 0.574) between short-term and long-term DAPTs. However, there was a significant difference in the risk of major bleeding (RR, 0.64; 0.49-0.85; P = 0.002) between the 2 groups. Compared with long-term DAPT, the short-term DAPT can reduce the risk of major bleeding without increasing the risk of death or ischemia for East Asians (Registered by PROSPERO, CRD42020213266).

We describe Cu-catalyzed intermolecular alkynylation and allylation of unactivated C(sp3)-H bonds with singly occupied molecular orbital-philes (SOMO-philes) via hydrogen atom transfer (HAT). Employing N-fluoro-sulfonamide as a HAT reagent, a set of substituted alkene and alkyne compounds were synthesized in high yields with good regioselectivity and functional-group compatibility. Late-stage functionalization of natural products and drug molecules is also demonstrated.

Wheat powdery mildew (Pm) caused by Blumeria graminis f. sp. tritici (Bgt) is a widespread epidemic disease in wheat. Haynaldia villosa, a diploid wild relative of wheat, has been widely utilized in wheat breeding for Pm resistance as a broad-spectrum resistance (BSR) resource. A previous study reported a Serine/threonine protein kinase-V (Stpk-V) gene from H. villosa showing BSR to wheat Pm. To deduce the defense network regulated by Stpk-V, we performed temporal RNA-seq of Stpk-V transgenic line (OEStpk-V) and its receptor wheat variety Yangmai 158 during Bgt infection. Gene Ontology enrichment indicated ROS were rapidly activated in OEStpk-V at 1 h after inoculation (hai). The qRT-PCR confirmed the upregulated transcription of ROS related genes OxO and POD, and suppression of ROS scavenging genes SOD and CAT. Besides, four transcription factors genes (WRKY, MYB, ERF, NAC) related to the ROS defense pathway were also enriched. Kyoto encyclopedia of genes and genomes enrichment revealed a remarkable reprogramming of the Ca2+ signalling pathway in OEStpk-V at 1 and 3 hai. qRT-PCR confirmed significantly induced transcription of CDPKs, CALMs, CMLs, and CNGCSs in OEStpk-V. Weighted gene co-expression network analysis classified Stpk-V to the same module in which the Ca2+ signalling related genes were included. Our results indicated that the reprogramming of the ROS accumulation and Ca2+ signalling at initial infection stages plays a crucial role in the Stpk-V regulatory network during wheat-Bgt interaction.

Helical milling is applied to the machining of CFRP owing to its favorable characteristics such as low cutting forces and heat generation. However, the prediction of helical milling forces remains a problem in CFRP processing. In this study, a novel predictive cutting force model of helical milling for unidirectional CFRP was established. Based on the cutting parameters, the dynamic cutting angle for the fiber and the corresponding cutting states of the fiber during the cutting process were analyzed, and the number and length of cutting edges involved in cutting were calculated. Then, the cutting forces on the cutting-edge micro-element of the periphery edge and bottom cutting edge under two different cutting states were calculated by treating the fiber as an equivalent homogeneous material. The cutting force prediction model was obtained by taking this into consideration. Finally, the helical milling experiment for unidirectional CFRP was carried out. The results show that the model can effectively predict the cutting force.

Overusing nitrogen fertilizer not only pollute farmlands, but also make Nilaparvata lugens (Stal) less susceptible to pesticides. The current study investigated the relationship between nitrogen fertilizer application and buprofenzin resistance in N. lugens in rice by conducting a series of field efficacy tests and laboratory bioassays. The results showed that the efficacy of buprofezin decreased significantly with high level of nitrogen fertilizer application, compared to those with low or no nitrogen application in rice fields. In the laboratory, the resistance ratios of N. lugens to buprofezin, assayed at three concentrations of nitrogen (0, 1.0, 3.0 mmol/L), have developed to 14.05-, 18.40- and 25.81-fold after 20 generations of resistance selection, respectively. Meanwhile, the resistance ratios of N. lugens without buprofezin treated were 1.00-, 3.30- and 4.67-fold after 20 generations as the nitrogen concentrations increased. The enzymatic assays revealed that the activities of P450 and esterase increased significantly after resistance selection with buprofezin and nitrogen, especially on the esterase activity in N. lugens under the condition of nitrogen treatment. These results suggested that nitrogen fertilizer application contributed to the resistance development in N. lugens to buprofezin by enhancing the activity of esterase.

In the drilling process of difficult-to-cut materials, conventional drilling has resulted in various problems such as high drilling temperature and poor machining quality. Low-frequency vibration-assisted drilling has great potential in overcoming these problems since broken chips are generated. In order to promote the application of low-frequency vibration-assisted drilling device in machining difficult-to-cut materials. In this paper, a low-frequency vibration-assisted drilling device is developed by using a novel ring flexure hinge as the elastic recovery mechanism. First, based on the theory of elastic mechanics and mechanical vibration, the stiffness of the ring flexure hinge is designed theoretically, and the influence of its structural parameters on its deflection is analyzed. And then the correctness of the theoretical design is further verified by static and dynamic simulation and stiffness test. Finally, the vibration performance of the device is tested under no-load condition, and the actual drilling test is conducted to verify the drilling performance. The results show that the device could realize the axial low-frequency vibration with constant frequency-to-rotation ratio and amplitude stepless adjustment and present good working stability under no-load and load conditions. In the actual drilling test of titanium alloy and carbon fiber reinforced plastic (CFRP)/ titanium alloy laminated structure, the device under appropriate processing parameters breaks the titanium alloy chip into small pieces and reduces the drilling temperature by 44% and inhibits the secondary damage of CFRP. It is demonstrated that the device could meet the actual processing requirements. And it also provides guidance for the design of low-frequency vibration-assisted drilling device.

Physicochemical properties, bacterial communities, and volatile compounds of mutton sausage produced from Jianzhou Big-Eared goat meat during natural ripening were investigated. Firmicutes and Bacteroidetes accounted for over 66% of all operational taxonomic units (OTUs) throughout the whole process, with Lachnospiraceae_NK4A136_group and Staphylococcus as the predominant genus during the early and later ripening periods, respectively. The evolution of microbial composition became less rich and diverse. The uncultured bacterium, the Lachnospiraceae_NK4A136_group, and Staphylococcus were marker bacteria on days 0, 7, and 26, respectively, with none on day 16. The bacteria distribution seemed to influence the volatile profile of mutton sausage throughout processing, with the bacterial composition on day 0 and day 7 clustered separately from day 16 and day 26, and the same pattern for the volatile profile. Meanwhile, the concentration of total volatile fraction significantly increased, and the majority of the volatile compounds were generated during late ripening. Non-anal, hexanal, decanal, heptanal, dodecyl aldehyde, benzaldehyde, 3-methylbutanal, γ-dodecalactone, 2-pentylfuran, and 1-octen-3-ol were key volatile compounds, contributing to the overall mutton sausage flavors. Based on Spearman’s correlation analysis, Staphylococcus as well as Psychrobacter were positively correlated with the production of the key volatile compounds, and other bacteria such as Lachnospiraceae_NK4A136_group, Bacteroides, Lactobacillus, Prevotella_1, Odoribacter, and so on were associated with the production of most alcohols and esters.

Insects entertain intricate and mutualistic relationship with an array of microorganisms, which significantly influence their fitness, ecology and evolution. In recent decades, there has been increasing interest toward studying the effects of microbiome on many host insects (Dipterans, Lepidopterans, and Coleopterans). Studies so far realized indicate that gut microbiome contribute to host nutritional ecology, defense, immunity and lifespan. Bactrocera dorsalis (Tephritidae: Diptera) is a polyphagous fruit fly which attacks a huge variety of fruits and vegetables worldwide and has been placed as a quarantine species by many countries. To investigate the specific functions of the gut endosymbionts, it is a prerequisite to know the composition of gut bacterial communities whose manipulation will help to decipher their ecological relevance. Here, we used the culture-dependent technique to isolate and identify gut bacteria from B. dorsalis at different developmental stages. The results revealed 11 bacterial species from the third instar larvae, 18 and 12 from female and male populations, respectively. These bacteria were assigned to six families, namely, Enterobacteriaceae, Enterococcaceae, Staphylococcaceae, Streptococcaceae, Micrococcaceae and Bacillaceae. Bacterial species from these families were differentially represented in various samples, except Klebsiella oxytoca , Enterobacter cloacae, Pantoea dispers and Enterococcus faecalis that were detected at all developmental stages. Overall, Enterobacteriaceae was the most dominant family in females and third instar larvae accounting for 57.89% and 26.32%, respectively, while Enterococcaceae was dominant in males with 75% of the total bacterial taxa. These results suggest that B. dorsalis possesses a huge variety of cultivable bacteria that could be used to explore their specific functions on host physiology and fitness.

BACKGROUND Genetic manipulation of sex determination pathways in insects provides the basis for a broad range of strategies to benefit agricultural security and human health. The P-element somatic inhibitor (PSI) protein, an exon splicing silencer that promotes male-specific splicing of dsx, plays a critical role in male sexual differentiation and development. The functions of PSI have been characterized in the lepidopteran model species Bombyx mori. However, the molecular mechanism and functions of PSI in Plutella xylostella, a worldwide agricultural pest and taxonomically basal species, are still unknown. RESULTS Here we identified PxPSI transcripts and analyzed their spatiotemporal expression pattern in P. xylostella. Multiple sequence alignment revealed that PxPSI contains four KH domains and is highly conserved in lepidopterans. We used the CRISPR-Cas9 system to generate mutations of the PxPSI genomic locus. Disruptions of PxPSI caused male-specific defects in internal and external genitals. In addition, we detected female-specific Pxdsx transcripts in PxPSI male mutants. Mutations also caused changes in expression of several sex-biased genes and induced male sterility. CONCLUSION Our study demonstrates that PxPSI plays a key role in male sex determination in P. xylostella and suggests a potential molecular target for genetic-based pest management in lepidopteran pests. © 2021 Society of Chemical Industry.

Specimens with single-phase martensite, net-like γ/martensite mixed structure and lamella-like γ/martensite mixed structure were designed to investigate the effect of the γ phase on the mechanical behavior and the detwinning of non-modulated (NM) martensitic variant in Ni-rich Ni-Mn-Ga alloys under uniaxial compression. It can be found the existence of the γ phase significantly enhances the compressive stresses, and the net-like γ phase specimen presents a higher value of compressive strain than that of the lamella-like γ phase specimen. Especially, the detwinning plateau of the lamella-like γ phase specimen is almost invisible due to the martensite colonies with low Schmid factors. Finally, according to the calculation of deformation gradient tensor, we found that the tensors along compression direction ( e z z ) of net-like γ phase/martensite mixed structure specimen and single-phase martensite specimen are lower than that of the specimen with lamella-like γ phase/martensite mixed structure, which well explained the detwinning strain for these specimens. The present study not only highlights the role of γ phase on the mechanical behavior, but also provides more guidelines for the mechanical training of Ni-Mn-Ga shape memory alloys.

A case who revealed the longest duration of viral shedding (67 days) in current reports, presented complicated characteristic on the relapse of COVID-19 due to the inconsistent performance of chest radiography and SARS-CoV-2-RNA detection after discharge. Lopinavir-interferon α2b boosted ribavirin following with lopinavir boosted budesonide might be a potent treatment for viral clearance.

The Vip3A, a vegetative Bacillus thuringiensis (Bt) gene, has been introduced into many Cry maize and cotton varieties that can manage the recently occurred Cry1/Cry2 resistance in Noctuidae pests including Helicoverpa zea (Boddie) in the USA. A seed blend refuge has been used for providing susceptible insect populations for Bt maize resistance management. Four field trials were deployed in this study to investigate the effects of Bt protein contamination due to gene flow in seed blend refuges of Viptera maize containing Cry1Ab and Vip3Aa20 genes with 0–30% refuges on the survival, development, and reproduction of the parental and F1 generations of H. zea. Viptera maize is highly effective against H. zea and likely expresses a ‘high dose’ for the insect. Compared to the survival on the structured refuge, seed blends reduced approximately 70% of the pupal and adult productions from refuge ears, and the reductions were not related to the refuge percentage. Pupae from the seed blend refuge weighed 22.1% less than those from the structured refuge, but the body mass reduction did not significantly affect the reproduction per female. All field populations were highly resistant to Cry1Ab, but susceptible to Vip3Aa20. Fitness of the F1 generations from the structured and seed blend refuges in diet rearing was generally similar. Data generated from the study can be used in simulation modeling to evaluate the feasibility of seed blends for resistance management.

Technological, safety-related and volatile properties were analyzed in coagulase-negative staphylococci (CNS) isolates from Chinese spontaneously fermented meat products. A total of 107 CNS isolates were identified via 16 S rRNA sequencing, and the most recovered species were S. saprophyticus (53.3 %), S. edaphicus (12.1 %), and S. epidermidis (10.3 %). Among them, 58 CNS isolates belonging to 9 species were selected with higher activities of catalase, nitrate reductase, proteolysis, and lipolysis, as well as higher tolerance to stressful environmental conditions. Then, 7 CNS isolates belonging to 4 species were further selected based upon excellent technological characteristics, lack of hemolysis and antibiotic resistance, and a low production of biogenic amines. The volatile profiles of these 7 strains cultivated in pork broth was determined. S. casei No. 1 produced significant amounts of phenethyl alcohol, geraniol, and 3-methyl-butanol. S. xylosus No. 120 produced the highest amount of methyl ketones with the potential to provide dry-cured odor of fermented meats. The volatile profile was highly strain dependent. Several CNS identified in this study have the potential to be used as the starter cultures for fermented meat products.

This paper proposes a new image encryption algorithm based on chaos map systems, hash algorithm, and Josephus permutation. The algorithm consists of chaos initialization, pixel position permutation, and pixel information diffusion. The algorithm’s initialization is generated by the original image, which has a high sensitivity to the initial value. The permutation step length is composed of Josephus permutation and gray curve permutation, which completely disturbs the pixel distribution. The diffusion process is composed of cross operation and ciphertext feedback, which breaks the strong correlation between pixels. The simulation results of the encryption algorithm are used to analyze its information entropy, the correlation between elements, and other indicators. The ciphertext image is attacked in several ways, and we analyzed its defense ability. Simulation results show that the algorithm can effectively encrypt image information and has a good defense against various attacks.

The DNA sequence design is a vital step in reducing undesirable biochemical reactions and incorrect computations in successful DNA computing. To this end, many studies had concentrated on how to design higher quality DNA sequences. However, DNA sequences involve some thermodynamic and conflicting conditions, which in turn reflect the evolutionary algorithm process implemented through chemical reactions. In the present study, we applied an improved multi-objective particle swarm optimization (IMOPSO) algorithm to DNA sequence design, in which a chaotic map is combined with this algorithm to avoid falling into local optima. The experimental simulation and statistical results showed that the DNA sequence design method based on IMOPSO has higher reliability than the existing sequence design methods such as traditional evolutionary algorithm, invasive weed algorithm, and specialized methods.

The classical Sonogashira reaction of aryl electrophiles in the presence of Pd catalysts has been well established as a potent method for arylalkyne synthesis. However, the site-selective C(sp2)–C(...

This paper puts forward an image encryption approach using chaotic maps and genetic operations. First, the Keccak algorithm is employed to compute the hash values of a plain-image as the initial values for chaotic map. The sensitivity and pseudo randomness of chaotic map used for the initial conditions allows for pseudorandom sequences to be obtained by iterative logistic map to shuffle and permute the pixel positions and values of the image. Second, in combination with the Henon map and the DNA coding technique, genetic operations at the bit level are used to achieve pixel selection, crossover and mutation, as well as further completion of pixel diffusion and scrambling, which significantly increases the difficulty of deciphering the algorithm. Finally, the diffusion and confusion features of the algorithm are further strengthened by bidirectional exclusive OR operations with chaotic sequences. The theoretical analysis and simulation results indicate that the algorithm is sensitive to keys and can effectively defend statistical and differential attacks, indicating that it has good security and application potential.

Creation of leaf color mutants is the options in the study of photosynthesis, chloroplast development and Chlorophyll biosynthesis. In this study, a leaf color mutant NAU31 was identified from tissue culture of common wheat. In general, the mutant exhibited whole plant yellowing, fewer tillering, dwarfing and interestingly, improved resistance to wheat powdery mildew. Photosynthetic physiological analysis showed that the chloroplast inner structure varied in the mesophyll cells of the mutant, and this may lead to reduced chlorophyll content (chlorophyll “a”, Chlorophyll “b”, total chlorophyll and carotenoid), as well as decreased maximum quantum efficiency of photosystem II (Fv/Fm) and net photosynthetic rate. Transcriptome analysis indicated that the impairing of photosynthesis may be responsible for plant yellowing of NAU31. Inheritance analysis showed that NAU31 was a gain-of-function mutant and the yellowing trait was controlled by a dominant gene, namely Taymg (yellow mutant gene). SSR markers analysis preliminarily mapped Taymg to the distal short arm of chromosome 2D using an F2 population from the cross between NAU31 and W7984, a synthesized wheat. The mapping result was confirmed by BSA using a 55 K wheat SNP array. Through recombinants screening, the Taymg was finely mapped to the terminal region of 2DS, flanked by markers ZH47 and gwm296 and corresponding to a physical interval of 0.76 Mb. The cloning of Taymg will facilitate the dissection of the mechanism involved in chloroplast development of wheat and the coordinating of plant development and disease resistance.

Objective To investigate the relationship between serum 25-hydroxyvitamin D [25(OH)D] levels and blood glucose and lipid levels in people over 18 years of age in Gansu, China. Subjects and Methods A total of 1928 volunteers (958 males and 970 females) were selected. The prevalence of abnormal glucose metabolism and lipid metabolism in the vitamin D deficiency group (

GC/MS coupled metabolomics analysis, using a simplified and much less expensive silylation process with trimethylsilyl cyanide (TMSCN), was conducted to investigate metabolic abnormalities in stomach cancer cells. Under optimized conditions for derivatization by TMSCN and methanol extraction, 228 metabolites were detected using GC/MS spectrometry analysis, and 89 metabolites were identified using standard compounds and the NIST database. Ten metabolite levels were found to be lower in stomach cancer cells relative to normal cells. Among those ten metabolites, four metabolites-ribose, proline, pyroglutamic acid, and glucose-were known to be linked to cancers. In particular, pyroglutamic acid level showed a drastic reduction of 22-fold in stomach cancer cells. Since glutamine and glutamic acid are known to undergo cyclization to pyroglutamic acid, the 22-fold reduction might be the actual reduction in the levels of glutamine and/or glutamic acid-both known to be cancer-related. Hence, the marked reduction in pyroglutamic acid level might serve as a biomarker to aid early detection of stomach cancer. Graphical abstract.

Harris hawks optimization (HHO) is a new swarm intelligence optimization technique. Because of its simple structure and easy to implement, HHO has attracted research interest from scholars in different fields. However, the low population diversity and the single search method in the exploration phase weakened the global search capability of the HHO algorithm. In response to these defects, this paper proposes an improved HHO algorithm based on adaptive cooperative foraging and dispersed foraging strategies. First, the adaptive cooperative foraging strategy uses three random individuals to guide the position update, which achieves cooperation between individuals. Then the cooperation behavior is embedded in the one-dimensional update operation framework, and the one-dimensional or total-dimensional update operation is adaptively selected. This way allows the algorithm to perform position update operations for a specific dimension of individual vectors with a certain probability, which improves the population diversity. Second, the dispersed foraging strategy is introduced into the HHO, forcing a part of Harris hawks to leave their current position to find more prey to obtain a better candidate solution. This way effectively avoids the algorithm falling into local optimum. Finally, a randomly shrinking exponential function is used to simulate the energy change of the prey, so that the algorithm maintains the exploration ability in the later exploitation process, effectively balancing the exploration and exploitation ability of the algorithm. The performance of the proposed ADHHO algorithm is evaluated using Wilcoxon's test on unimodal, multimodal and CEC 2014 benchmark functions. Numerical results and statistical experiments show that ADHHO provides better solution quality, convergence accuracy and stability compared with other state-of-the-art algorithms.

MnO2–CoOX catalysts with different Mn/Co ratios were prepared using polyvinyl pyrrolidone (PVP) as an assistant to control the morphology and exposed crystal faces of composite oxides. The physicochemical properties of the catalysts were systematically characterized and the catalytic activity of NH3-SCR and resistance to SO2/H2O were investigated. It was found that the NO conversion over Mn–Co-0.2-PVP was apparently higher compared with that over Mn–Co-X-PVP (X = 0.9, 0.8, 0.6, and 0.4). This can be interpreted in terms of exposed crystal surface (110) and enhanced adsorption of NH3/NO, and better redox performance resulting from higher Co3+/Co2+ ratio. Furthermore, different PVP and HAc additives have different H2O and SO2 resistance. Mn–Co-0.2-PVP showed good resistance to H2O and SO2 poisoning owing to having more low-temperature (∼200 °C) NO adsorption sites, which are beneficial to promote the de-NOX reaction and less affected by SO2.

Background: Traditional angiography only displays two-dimensional images of the coronary arteries during stent implantation. However, intravascular imaging can show the structure of the vascular wall, plaque characteristics. This article aims to evaluate the efficacy of intravascular imaging-guided stent implantation.Method: We conducted a systematic review and meta-analysis of randomized controlled trials of intravascular imaging-guided, including patients with stent implantation guided by intravascular ultrasound (IVUS) or optical coherence tomography (OCT) and traditional angiography. The databases of PubMed, EMBASE, web of science, and Cochrane Library were searched. The primary outcome was target lesion revascularization (TLR). The secondary outcomes included the target vessel revascularization (TVR), myocardial infarction (MI), stent thrombosis (ST), cardiac death, all-cause death, and the major adverse cardiac events (MACE) during the 6-24 months follow-up. The fixed-effects model was used to calculate the relative risk (RR) and 95% confidence interval (CI) of the outcome event. Meanwhile, the trial sequence analysis was employed to evaluate the results.Result: This meta-analysis included eighteen randomized controlled trials with 10413 patients. Compared with angiography-guided stent implantation, intravascular imaging-guided stent implantation can significantly reduce the risk of TLR (RR 0.62, 0.49-0.77, P＜0.0001), TVR (RR 0.65, 0.52-0.81, P = 0.0001), cardiac death (RR 0.59; 0.39-0.91; P =0.02) , ST (RR 0.54, 0.30-0.97; P = 0.04) and MACE (RR 0.81, 0.71-0.93; P = 0.003), while there was no significant difference regarding MI (RR 0.78, 0.61-1.00, P = 0.05) and all-cause death (RR 0.85, 0.58-1.27, P = 0.44).Conclusions: Compared with angiography, intravascular imaging-guided stent implantation is associated with better clinical outcomes in patients with coronary artery disease (Registered by PROSPERO, CRD 42021289205).

Background Traditional angiography only displays two-dimensional images of the coronary arteries during stent implantation. However, intravascular imaging can show the structure of the vascular wall, plaque characteristics. This article aims to evaluate the efficacy of intravascular imaging-guided drug-eluting stent (DES) implantation. Method We conducted a systematic review and meta-analysis of randomized controlled trials of intravascular imaging-guided, including patients with DES implantation guided by intravascular ultrasound or optical coherence tomography and traditional angiography. The databases of PubMed, EMBASE, web of science, and Cochrane Library were searched. The primary outcome was target lesion revascularization (TLR). The secondary outcomes included the target vessel revascularization (TVR), myocardial infarction (MI), stent thrombosis (ST), cardiac death, all-cause death, and the major adverse cardiac events (MACE) during the 6–24 months follow-up. The fixed-effects model was used to calculate the relative risk (RR) and 95% confidence interval of the outcome event. Meanwhile, the trial sequence analysis was employed to evaluate the results. Result This meta-analysis included fourteen randomized controlled trials with 7307 patients. Compared with angiography-guided, intravascular imaging-guided DES implantation can significantly reduce the risk of TLR (RR 0.63, 0.49–0.82, P = 0.0004), TVR (RR 0.66, 0.52–0.85, P = 0.001), cardiac death (RR 0.58; 0.38–0.89; P = 0.01), MACE (RR 0.67, 0.57–0.79; P P = 0.005). While there was no significant difference regarding MI (RR 0.77, 0.57–1.05, P = 0.10) and all-cause death (RR 0.87, 0.58–1.30, P = 0.50). Conclusions Compared with angiography, intravascular imaging-guided DES implantation is associated with better clinical outcomes in patients with coronary artery disease, especially complex lesions (Registered by PROSPERO, CRD 42021289205).

The corn earworm/bollworm

OBJECTIVE: To study the association of the anti-oxidative damage factors nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NAD(P)H:quinone oxidoreductase-1 (NQO1) with preterm premature rupture of membranes (PPROM). METHODS: A prospective study was conducted. The neonates who were hospitalized in Yanbian Hospital from 2019 to 2020 were enrolled as subjects, among whom there were 30 infants with PPROM, 32 infants with term premature rupture of membranes (TPROM), and 35 full-term infants without premature rupture of membranes (PROM). Hematoxylin and eosin staining was used to observe the inflammatory changes of placental tissue. Immunohistochemical staining was used to measure the expression of Nrf2, HO-1, and NQO1 in placental tissue. Western blot was used to measure the protein expression levels of Nrf2, HO-1, and NQO1 in placental tissue. RESULTS: Compared with the PPROM group, the TPROM group and the non-PROM full-term group had significantly higher positive expression rates and relative protein expression levels of Nrf2, HO-1, and NQO1 in placental tissue (P0.05). CONCLUSIONS: The low expression levels of Nrf2, HO-1, and NQO1 in placental tissue may be associated with PPROM, suggesting that anti-oxidative damage is one of the directions to prevent PPROM.

Tubulin affects platelets count through the control of mitosis and the formation of pro-platelets during the maturation of megakaryoblast to platelets. Tubulin is involved in maintaining the integrity of platelet skeleton, and also participates in the change of platelet morphology during platelet activation. Some new anti-tumor drugs targeting cell mitosis are trying to reduce the effect on tubulin in order to reduce the side effect of drugs on platelet formation. In some patients with thrombocytopenia, the variation and polymorphism of the tubulin gene affect the structure of microtubule multimers, which leads to the decrease of platelet formation. This review summarized the latest progresses of tubulin in the regulation of megakaryopoiesis and thrombopoiesis.微管蛋白在巨核细胞发育和血小板数量调控中的 作用机制研究进展. 微管蛋白在巨核祖细胞向血小板发育成熟的过程中通过对有丝分裂的控制和原血小板的形成影响血小板的数量。在血小板中微管蛋白参与维持血小板骨架的完整，并在血小板活化过程中参与血小板形态的改变。一些以细胞有丝分裂为靶向的抗肿瘤新药正试图有针对性地减少对微管蛋白的影响以减轻药物对血小板生成的抑制作用。临床上一些血小板减少症患者由于微管蛋白基因的变异和多态性影响了微管多聚体的结构从而导致血小板生成减少。本文对微管蛋白在巨核细胞发育成熟和血小板生成调控过程中的最新研究进展作一综述。.