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T-2 toxin treatment causes male reproduction system dysfunction, although the exact mechanism remains unclear. In this research, male Kunming mice and TM4 cells were treated with varying concentrations of the T-2 toxin for evaluating the adverse effect of T-2 toxin on male reproductive function. MCC950 or NAC was used to block NLRP3 inflammasome activation and eliminate reactive oxygen species (ROS) accumulation in the TM4 cell, respectively. The results showed that: (1) T-2 toxin caused testicular atrophy, destroyed the microstructure and ultrastructure of the testis, and caused sperm deformities; (2) T-2 toxin increased the content and gene expressions of TNF-α and IL-6 and decreased the IL-10 content and gene expression, causing testis and TM4 cell inflammatory injury; (3) T-2 toxin activated NLRP3 inflammasome in the testis and TM4 cells and caused ROS accumulation in the testis; (4) suppressing NLRP3 inflammasome activation using 20 nM MCC950 alleviated the TM4 cell inflammatory damage caused via the T-2 toxin; nevertheless, 20 nM MCC950 did not reduce ROS accumulation in TM4 cells; and (5) NAC relieved the inflammatory damage in TM4 cells by inhibiting NLRP3 inflammasome activation. Taken together, T-2 toxin caused testicular inflammation injury through ROS-mediated NLRP3 inflammasome activation, resulting in male reproductive dysfunction.

Iron is an important metal element involved in the regulation of male reproductive functions and has dual effects on testicular tissue. A moderate iron content is necessary to maintain testosterone synthesis and spermatogenesis. Iron overload can lead to male reproductive dysfunction by triggering testicular oxidative stress, lipid peroxidation, and even testicular ferroptosis. Ferroptosis is an iron-dependent form of cell death that is characterized by iron overload, lipid peroxidation, mitochondrial damage, and glutathione peroxidase depletion. This review summarizes the regulatory mechanism of ferroptosis and the research progress on testicular ferroptosis caused by endogenous and exogenous toxicants. The purpose of the present review is to provide a theoretical basis for the relationship between ferroptosis and male reproductive function. Some toxic substances or danger signals can cause male reproductive dysfunction by inducing testicular ferroptosis. It is crucial to deeply explore the testicular ferroptosis mechanism, which will help further elucidate the molecular mechanism of male reproductive dysfunction. It is worth noting that ferroptosis does not exist alone but rather coexists with other forms of cell death (such as apoptosis, necrosis, and autophagic death). Alleviating ferroptosis alone may not completely reverse male reproductive dysfunction caused by various risk factors.

Toxoplasmosis is one of the most common zoonotic diseases in the world. Felines excrete

Distributed storage systems (DESSs) are widely utilized to regulate voltages in active distribution networks with high penetration of volatile renewable energy. In this paper, the distributed multi-energy storage systems (MESSs) are integrated into the active distribution network to enhance the capability of voltage regulation by exploiting interactions among multi-energy loads. A novel distributed control strategy based on back-and-forth communication (BFC) framework is developed to optimally coordinate multiple MESSs for multi-time-step voltage regulation. To achieve the independent control of MESSs at each bus, the BFC is proposed to determine the global sensitivities of voltage violation in the whole network with respect to the power injection at each bus in only one-step iteration. These sensitivities updated in real time during BFC quantify the capability of the single MESS to mitigate voltage fluctuations across all buses, which can be used to individually inform the operation of each MESS. Distinct from traditional distributed control methods that achieve voltage regulation in a single timeslot, the proposed control strategy regulates MESSs in a multi-time-step manner to alleviate anticipated voltage violation in advance. The simulation based on a modified 11-bus radial distribution network demonstrates the effectiveness of the proposed distributed control strategy.

T-2 toxin, the most toxic type A trichothecene mycotoxin, is produced by Fusarium, and is widely found in contaminated feed and stored grains. T-2 toxin is physicochemically stable and is challenging to eradicate from contaminated feed and cereal, resulting in food contamination that is inescapable and poses a major hazard to both human and animal health, according to the World Health Organization. Oxidative stress is the upstream cause of all pathogenic variables, and is the primary mechanism through which T-2 toxin causes poisoning. Nuclear factor E2-related factor 2 (Nrf2) also plays a crucial part in oxidative stress, iron metabolism and mitochondrial homeostasis. The major ideas and emerging trends in future study are comprehensively discussed in this review, along with research progress and the molecular mechanism of Nrf2’s involvement in the toxicity impact brought on by T-2 toxin. This paper could provide a theoretical foundation for elucidating how Nrf2 reduces oxidative damage caused by T-2 toxin, and a theoretical reference for exploring target drugs to alleviate T-2 toxin toxicity with Nrf2 molecules.

Combined heat and power dispatch (CHPD) is utilized for energy-efficient coordination of integrated electricity and heat systems. By incorporating district heating networks (DHNs) into CHPD, the thermal storage capability of network pipelines can be exploited to increase the operational flexibility for high penetration of renewable energy. This article proposes a flexibility evaluation method based on a generalized thermal storage model to systematically characterize and quantify the flexibility of DHNs in CHPD. In particular, systematic flexibility metrics are introduced to define the parameters of the proposed thermal storage model, including heat ramping capability, heat input limits, and heat storage capacities. A direct quantification method is proposed to explicitly derive these metrics without extensive simulations required in traditional methods. Besides, four different control modes of district heating systems are identified and modeled according to the controllability of mass flow rates and supply temperature at heat sources. A simplified CHPD model is developed based on sequential linear programming and a lumped heat loss model to enhance the computational efficiency. Comprehensive case studies validate the effectiveness of the proposed method in evaluating the flexibility of DHNs in CHPD. It is demonstrated that the flexibility of DHNs in CHPD can be exploited as far as possible in the control mode with adjustable mass flow and supply temperature.

Increasing deployment of distributed energy resources intensifies interactions among electric, gas, heating, and cooling systems. Inherent uncertainties ranging from internal parameters to external inputs also impose significant challenges on system operation. A coordinated scheduling method is, therefore, desired for facilitating efficient operation of multi-energy microgrids under multiple uncertainties. In this paper, a deterministic coordinated scheduling model is developed first with detailed modeling of system dynamics and device parameters to fully characterize complex interactions among multi-energy carriers. Then, a hybrid stochastic-interval method is proposed to account for heterogeneous uncertainties in decisions of operational strategies. Specifically, performance and efficiency uncertainties of distributed energy resources, and injection uncertainty of renewable sources and multi-energy demands are characterized by interval-based uncertain operation regions and probability distributions, respectively. A scenario-based two-stage algorithm is developed to solve the problem so that multiple uncertainties are preserved in the entire decision-making process. Moreover, adjustable operational strategies are determined to hedge uncertainty by considering operators’ risk preferences. The validity of the proposed method is verified by comprehensive case studies on a test system.

The present study showed that 4.40% (15/341) of the dogs were seropositive forA total of 4.29% dogs were infected by

Marsupials are highly susceptible to Toxoplasma gondii infection. Here, we report T. gondii infection in four kangaroos from a zoo in China. Kangaroos were imported into China in 2000 and were since bred in zoo. In 2017–2018, four kangaroos died due to respiratory system disease or injury. The bodies were submitted to the laboratory to test for T. gondii infection. Antibodies to T. gondii were found in 75% (3/4) of the kangaroos via the modified agglutination test with the cut-off 1:25. Cysts were observed in the histopathological sections of tongue and diaphragm or squashes of fresh myocardium in two kangaroos. These cysts were confirmed as T. gondii by immunohistochemical staining and molecular biological analysis. One viable T. gondii strain was isolated from one kangaroo and designated as TgRooCHn1. DNA from T. gondii tachyzoites obtained from cell culture was characterized by 10 PCR-RFLP markers and the virulence genes ROP5 and ROP18. The genotype of this isolate did not match with any known genotypes; it was designated as ToxoDB#292. The virulence of TgRooCHn1 (104 tachyzoites) was non-lethal to mice, and it formed tissue cysts. To our knowledge, the present study is the first isolation of ToxoDB#292 strain from kangaroo. Improvemets for captive settings were initiated, including greater attention being paied to birds and stray cats, fed frozen meat for carnivores., Graphical abstract Image 1, Highlights • T. gondii infection in kangaroo was confirmed by serological and molecular analysis, histopathology, and bioassay in mice. • One viable T. gondii strain (ToxoDB#292) was isolated from one kangaroo. • The virulence of TgRooCHn1 in mice was low and it formed tissue cysts.

High penetration of distributed renewable energy introduces significant uncertainties to active distribution networks. Optimal control methods accounting for inherent uncertainties are needed to facilitate economic and reliable operation of active distribution networks. This paper proposes a stochastic receding horizon control method based on modified stochastic model predictive control framework to integrate high penetration of distributed generation. Multiple controllable resources are jointly optimized over a finite prediction horizon while ensuring relevant security restrictions. The simplified Z-bus sensitivity for active distribution networks is developed for computationally efficient estimation of system nonlinearity with high accuracy, and is combined with the sequential linear programming to iteratively derive the linear state space model for compensation of cumulative modeling errors. Furthermore, the voltage limitations are reformulated as chance constraints to indicate the probabilistic reliability index of voltage qualification rate, and achieve tradeoffs between cost reduction and voltage regulation. The affine-disturbance feedback control policy is leveraged here to enforce close-loop control performance and analytically transform intractable chance constraints into second-order cone constraints. Comprehensive case studies based on 33-bus and 123-bus distribution systems are carried out to demonstrate the capability and effectiveness of the proposed approach in terms of modeling accuracy, control performance, cost reduction, and method scalability. The proposed approach can effectively enforce voltage regulation against uncertainties with the prescribed probability level. Control costs and constraint violation can be reduced compared with deterministic model predictive control and open-loop control strategies.

District heating system is a high efficient method for providing heat to buildings and is intensively coupled with power system. More multi-energy microgrids are connected to district integrated heating and distribution networks centrally managed by an energy service company (ESCO). Given the fact that microgrid clusters and district integrated heating and distribution networks belonging to different entities, this paper develops a hierarchical autonomous cooperative operation model to minimize total cost and improve calculation efficiency without transparent private information. The original problem is separated into twolevel with a master problem and a series sub-problems. In the upper level, ESCO operates the district integrated heating and distribution network with consideration of pipeline energy storage and heat transmission delay. While local operators (LO) that utilize the thermal inertia of buildings autonomously control energy conversion and flow in the lower level. Based on analytical target cascading (ATC) theory, interface electrical power and thermal energy power are modeled as a pseudo source and pseudo load to decoupling multi sectors. Numerical experiments are implemented to validate the effectiveness and superior convergence of the proposed approach.

Myocardium and diaphragm samples of cattle (n = 521) from HeNan Province (China) were screened for Sarcocystis sarcocysts by histological examination, pepsin digestion, and molecular assays. Morphology and molecular assays were used for identification. The prevalence of Sarcocystis infection in cattle was 41.5% (216/521). Histological examination identified sarcocysts in the myocardium (49.4%, 200/405) and diaphragm (13.8%, 16/116) of cattle. Two species were identified, namely S. cruzi (41.3%, 215/521) and S. hominis (0.2%, 1/521). The findings of the present study indicate a high prevalence of S. cruzi infection in cattle from central China.

Toxoplasmosis is one of the most common foodborne diseases in the world. The objective of this study was to determine Toxoplasma gondii infection in lambs from Henan province, China. A total of 166 lamb hearts were collected from 2017 to 2019. T. gondii infection was determined by the Modified Agglutination Test (MAT) using heart juice of lambs. 11 isolates (TgSheepCHn3 - TgSheepCHn13) were obtained from samples with MAT titers ≥1:100. The rate of T. gondii isolation increased with antibody titer against T. gondii (P

Increasing deployment of combined heat and power plants intensifies interactions between renewable-based electric power systems and district heating systems. Combined heat and power dispatch is thus critical for energy-efficient coordination of integrated electricity and heat systems under high penetration of renewable energy. However, the complicated nature of district heat flows introduces significant nonlinearities and precludes the practical implementation. In this paper, a combined heat and power dispatch method based on a simplified district heat flow model is proposed to enhance the computational efficiency with desirable accuracy for better operational decisions. Several novel modifications are incorporated to reduce the model size and relieve the model nonlinearity through analyzing operational principles of district heating networks. An iterative solution algorithm is developed accordingly based on sequential linear programming to boost computational efficiency and guarantee fast convergence. The proposed method can describe temperature dynamics of district heating systems with respect to transmission delays and heat losses without assumptions on constant mass flow rates or constant supply temperature. Case studies based on a test system validate the effectiveness of the proposed method.

Pulmonary arterial remodelling is a pathological characteristic of pulmonary arterial hypertension (PAH), which contributes to the development of sustained pulmonary hypertension. The aim of this study was to investigate the effects of dietary Trifolium pratense isoflavones on pulmonary vascular remodelling in experimental broiler pulmonary hypertension syndrome. Exposure to sub-thermoneutral environmental temperatures increased broiler's pulmonary hypertension syndrome incidence and raised expression levels of nitric oxide, endothelin and endothelial nitric oxide synthase. Dietary supplementation (20 mg/kg basal diet) with Trifolium pratense isoflavones reduced pulmonary hypertension syndrome incidence and improved pulmonary vascular remodelling without affecting growth performance. The beneficial effect likely came from isoflavone improved pulmonary vascular remodelling. Isoflavone induced inducible nitric oxide synthase expression, which led to increased nitric oxide level. The nitric oxide could mediate vasorelaxation in the lungs. At the same time, the expression of endothelin was downregulated by isoflavone. Dietary supplementation of Trifolium pratense isoflavone might be a potential therapeutic strategy for the treatment of pulmonary hypertension.

The felids are the only definitive hosts of Toxoplasma gondii, which could excrete oocysts into the environment and provide an infection source for toxoplasmosis in various warm-blooded animal species, particularly the captive felids that live close to human communities. The infection rate of the captive felids is a perfect standard in detecting the presence of Toxoplasma gondii oocysts in the environment. In this study, sera or tissue samples from zoo (1 young tiger, 2 adult tigers, 6 young lions), farm (10 masked palm civets), and pet hospital (28 cats) from Henan Province (China) were collected. The sera (n = 47) were tested for immunoglobulin G (IgG) antibodies against T. gondii by using modified agglutination test (MAT), whereas the hearts tissue (n = 40) were bioassayed in mice to isolate T. gondii strains. The genotype was distinguished by using PCR-RFLP of 10 loci (SAG1, SAG2, SAG3, GRA6, BTUB, L358, c22-8, PK1, c29-2, and Apico). The detection rate for the T. gondii antibody in captive felids was 21.3% (10/47). One viable T. gondii strain (TgCatCHn4) was obtained from a cat heart tissue, and its genotype was ToxoDB#9. The oocysts of ToxoDB#9 were collected from a T. gondii-free cat. The virulence of TgCatCHn4 was low and no cysts were detected in the brain of mice at 60 days post-inoculation. The finding of the present study suggested a widespread exposure of T. gondii for felids in Henan Province of central China, particularly those from the zoological gardens and homes. ToxoDB#9 was the predominant strain in China. Preventive measures against T. gondii oocyst contamination of various components of the environment should thus be implemented, including providing pre-frozen meat, well-cooked cat food, cleaned fruits and vegetables, monitoring birds and rodents, inactive T. gondii oocysts in felids feces, and proper hygiene.

Defensins serve as alarm signals in mobilizing the immune system and activating the innate and adaptive immune responses. In order to investigate whether avian defensins could activate monocytes of another species, and whether chicken defensins could modulate or amplify the adaptive immune responses of murine through the TLR-NF-kappaB pathway, the relationship between the chicken intestinal defensin AvBD13 and TLR4 in murine peripheral blood mononuclear cells (PBMCs) was explored in vitro. Monocytes were stimulated with AvBD13 (1 microg/mL). The levels of NF-kappaB p65, CD80, CD86, IL-12 and IFN-alpha were measured by immunohistochemical analysis of the cells or enzyme-linked immunosorbent assay (ELISA), and the TLR4 levels in monocytes were measured by flow-cytometry. We found that AvBD13 can activate NF-kappaB, induce the inflammatory cytokines IL-12 and IFN-alpha, and upregulate costimulatory molecules like CD80 and monocyte proliferation, which was clearly inhibited by the anti-TLR4 antibody. TLR4 expression was rapidly downregulated in the presence of AvBD13. AvBD13 could modulate monocytes directly and serve as an endogenous ligand for TLR4 and upregulate costimulatory molecules and monocyte proliferation. Thus, TLR4 is involved in AvBD13-mediated activation of adaptive immune responses.

Red clover isoflavone already became a kind of health food and pharmaceuticals raw material. In order to study the effect of red clover isoflavone on antioxidant and immune performance of chicken under heat stress conditions, two hundred and forty 1-day-old AA chicken were randomly assigned to three groups, control, group 1 and group 2, basic diet, basic diet+red clover isoflavone (10 mg·kg−1) and basic diet+red clover isoflavone (20 mg·kg−1) were fed to the chicken respectively. The time of this experiment was five weeks. The results indicated that red clover isoflavone could increase the activity of GSH-PX and SOD in serum and liver, reduced the content of MDA, enhance the content of IgG, IgM and IL-2 in serum, and bear on TNF-α during heat stress. These results showed that red clover isoflavone could strengthen antioxidant and immunity, and lessen the impairing of heat stress on chicken.

Anatomic and histological structure of the cloaca in ostrich was investigated by gross appearance and light microscopy. The results indicated that, the cloaca of African ostrich was larger than chicken. Cloaca receiving waste from large intestine, urinary and reproductive systems, and was divided into three sections by three mucous plicas: coprodeum, vault-like urodeum and proctodeum. The epithelium of finger-like villi in the cloaca was simple columnar epithelium. The goblet cells of mucosa were abundant and showed globose apophysis. Lymphoid follicles were found in mucosa of cloaca.

Five-hundred 1-day-old broilers were randomly assigned to five groups, 100 chickens for each group. From group 1 to 3, 80, 120 and 200 mg/kg soybean peptides was added to the diets respectively; in the fourth group, 3.2 mg/kg genramycin was added; and the fifth group was the control without soybean peptides and antibiotics. At the age of 28 and 49 days, the number of goblet cells (GC), intestine intraepithelial lymphocyte, immunoglobulin A-forming cells, the ratio of villous height and crypt depth (V/C) of broiler's duodenum, jejunum and cecum were observed by the application of haematoxylin and eosin or histochemistry staining. The results indicated that soybean peptides added with 80-120 mg/kg could increase daily weight gain, the number of GC and V/C. Soybean peptides could modulate intestinal mucosal immunity of broilers.