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Additional file 1. Supplementary research data of human placental exosomes induce maternal systemic immune tolerence by reprogramming circulating monocytes.

Additional file 1: Table S1. Table S2. Figure S1. Figure S2. Figure S3. Figure S4. Figure S5. Figure S6.

Additional file 1: Figure S1. Expression of eMSC surface markers (CD140b & CD146) in clonally derived cells. Representative figures showing the gating strategy to evaluate the phenotypic markers of eMSCs (CD140b+CD146+ cells) using flow cytometry. (A) Clonally derived cells were gated on flow cytometric profile based on the forward scatter (FSC, associated with cell size) and side scatter (SSC, associated with cell granularity). (B) Single cells were separated from doublets and aggregated cells based on their SSC area (SSC-A) and SSC height (SSC-H) on the dot plot. (C) Single parameter histograms for individual markers: CD140b-PE+ cells and, CD146-FITC+ cells. Grey area indicates background fluorescence with isotype matched IgG control. The percentage of cell maintaining CD140b-PE+ and CD146-FITC+ on the upper right quadrant of the dot plot from (D) monoculture, (E) coculture with epithelial niche cells from the menstrual phase, (F) coculture with stromal niche cells from the menstrual phase. (G) Relative cloning efficiency eMSC colonies. in monoculture, coculture with OE E6/E7 or HFF-1 (n = 5). (H) Relative proportion of CD140b+CD146+ cells after coculture (n = 5). Data normalized to the monoculture group. (I) Relative proportion of CD140b+CD146+ cells in ctrl (white bar) and 12.5, 25, 50 ng/ml of rhWNT3A (grey bars). (J) Western blotting image and quantitative analysis of LGR5 expression in stromal cells after gene silencing with si-RNA (n = 3). Results are shown as mean ± SEM; *P