1. Capture-free deactivation of CTCs in the bloodstream; a metastasis suppression method by electrostatic stimulation of the peripheral blood
- Author
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Mahsa Faramarzpour, Mohammad Abdolahad, Mojtaba Jahangiri, Mohammad Esmaeil Akbari, Mohammad Ali Khayamian, Yasin Kordehlachin, Najmeh Dabbagh, Saeed Rafizadeh-Tafti, Hossein Simaee, Hadi Ghaffari, Parisa Hoseinpour, Mohammad Reza Esmailinejad, Mohammadreza Ghaderinia, Fereshteh Abbasvandi, Shahriar Shalileh, Ehsan Ansari, Hamed Abadijoo, and Ashkan Zandi
- Subjects
Side effect ,Static Electricity ,Biomedical Engineering ,Biophysics ,Breast Neoplasms ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Metastasis ,Metastasis Suppression ,Mice ,Breast cancer ,Cell Line, Tumor ,Electrochemistry ,medicine ,Animals ,Humans ,Neoplasm Metastasis ,Whole blood ,Lung ,business.industry ,010401 analytical chemistry ,Cancer ,General Medicine ,Neoplastic Cells, Circulating ,021001 nanoscience & nanotechnology ,medicine.disease ,Extravasation ,0104 chemical sciences ,medicine.anatomical_structure ,Cancer research ,0210 nano-technology ,business ,Biotechnology - Abstract
While limited investigations have been reported on CTC elimination and its profits, recently, some new works were reported on detection followed by the destruction of CTCs. Limitations and complications of CTC capturing procedures have highly reduced the chance of selective destruction of CTCs in the bloodstream in the therapeutic guidelines of the patients. Here, we selectively deactivated the invasive function of CTCs during their circulation in the bloodstream by exposing the whole blood to pure positive electrostatic charge stimulation (PPECS). Our treatment suppressed pulmonary metastasis and extended the survival of the mice had been intravenously injected by electrostatically deactivated 4T1 breast cancer CTCs. Moreover, the number of cancerous lung nodules was drastically reduced in the mice injected by treated CTCs in comparison with the non-treated cohort. Evaluating the side effect of the PPECS on the blood components revealed no major effect on the functional properties of the white blood cells, and just a negligible fraction (∼10%) was damaged during this process. This approach does not need any capturing or targeting of CTCs from the blood as it is focused on perturbing the electrical function of negatively-charged tumor cells after being exposed to positive electrostatic charges. Taken together, continuous in-vivo deactivation of CTCs by PPECS with no requirement to complicated capturing protocols may improve the survival of cancer patients.
- Published
- 2021