1. Tritiated Water Induces Toxicity in Human Umbilical Vein Vascular Endothelial Cells via IL8
- Author
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Zhen-Yan Li, Yan Hongbin, Yulong Liu, Pei-Jun Xue, Fengmei Cui, Yu Tu, Qiu Chen, Yi-Tong Liu, Kongzhao Wang, Meng Zhang, Zhuo-Jun Wu, and Yong-Ming He
- Subjects
human umbilical vein vascular endothelial cells ,Chemical Health and Safety ,Tritiated water ,Angiogenesis ,Health, Toxicology and Mutagenesis ,lcsh:RM1-950 ,Public Health, Environmental and Occupational Health ,In vitro toxicology ,Interleukin ,interleukin 8 ,Toxicology ,Umbilical vein ,030218 nuclear medicine & medical imaging ,Cell biology ,Extracellular matrix ,03 medical and health sciences ,chemistry.chemical_compound ,lcsh:Therapeutics. Pharmacology ,0302 clinical medicine ,chemistry ,030220 oncology & carcinogenesis ,Toxicity ,Original Article ,Interleukin 8 ,tritiated water - Abstract
We aimed to determine the toxic effects of tritiated water (HTO) on 12 generations (T1-T12) of human umbilical vein vascular endothelial cells (HUVECs) and elucidate the underlying mechanisms. We evaluated cellular senescence, interleukin (IL) 8 concentrations, and angiogenesis using β-galactosidase staining, enzyme-linked immunosorbent assay, and in vitro assays, respectively. The adhesion properties of contaminated cells and differentially expressed genes were assessed using the xCELLigence RTCA SP system and gene chip analysis, respectively. We found that long-term exposure to low levels of HTO can reduce the adhesion of HUVECs to the cellular matrix as well as their angiogenic capacity, while increasing their permeability, senescence, and adhesion to monocytes. Interleukin 8 activated the p38 and Epidermal Growth Factor Receptor (EGFR) pathways in HTO-treated cells and hence was identified as a key candidate of biomarker. The present study clarified the toxicity of HTO in vascular endothelial cells and identified IL8 as a novel protective target with important theoretical and practical values.
- Published
- 2020