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Purpose: To investigate the internal training loads of a professional Spanish female futsal team throughout 26 weeks of training including preseason and in-season weeks and verify the impact of training period and/or training load magnitudes on heart-rate variability responses. Furthermore, we aimed to assess, intraindividually, the relationship between training load and the coefficient of variation (CV) of weekly natural log of the root mean square difference of successive normal interbeat (RR) intervals (lnRMSSDCV), obtained from ∼5 measures per week, and recorded in the seated position. Methods: A within-subject design involved 12 high-level outfield female futsal players (mean [SD] age: 23.9 [3.4] y). Results: lnRMSSD was significantly lower and lnRMSSDCV was significantly higher during the preseason (weeks 1–6) compared to in-season (weeks 7–26) (P moderate to large negative correlations between lnRMSSDCV and lnRMSSD during the 26 weeks of observation. Correlations ranged between rplayer4 = −.41 (95% CI, −.69 to −.02) and rplayer12 = −.55 (−.78 to −.18). Players also presented moderate to very large positive correlations between lnRMSSDCV and session rating of perceived exertion. Correlations ranged between rplayer7 = .41 (.04 to .71) and rplayer1 = .71 (.45 to .86). Conclusion: Professional female futsal players in this study presented increased lnRMSSD and reduced lnRMSSDCV during 20 weeks into the competitive season compared to 6 weeks of preseason. Furthermore, lnRMSSDCV was negatively associated with lnRMSSD on an intraindividual basis. Finally, higher internal training loads were positively correlated with lnRMSSDCV, indicating that heart-rate variability is responsive to weekly training loads.

We have developed a novel radiopaque tiltmeter (ROT) that can indicate patient tilt during a radiography examination and display it on X-ray images. This study evaluated the effect of variation of patient tilt on the reproducibility of Fowler's position for chest radiography and the accuracy of the ROT.We evaluated the reproducibility of Fowler's position based on changes from the first day in the central venous catheter (CVC) tip position and the cardiothoracic ratio (CTR) with and without a digital tiltmeter to verify its efficacy in patients who underwent mobile chest radiography. The ROT contains radiopaque liquid consisting of white barium sulfate solution and oil and has a scale bar of 15°-75° with increments of 15° to indicate ROT tilt. The ROT tilt was increased from 10° to 80° in increments of 10°. We then evaluated (1) the difference between the ROT tilt and the tilt measured with a digital tiltmeter, and (2) the ROT tilt displayed on the X-ray image.With regard to reproducibility in Fowler's position, changes in the CVC tip position were 2.8 ± 3.9 mm and 10.7 ± 10.6 mm with and without the tiltmeter, respectively (p 0.05) and the respective rates of change in the CTR were 0.7% ± 0.6% and 4.0% ± 2.1% (p 0.05). Differences between the ROT tilt and the tilt measured by the digital tiltmeter were within ±2.5°. All ROT tilts displayed on the X-ray images were recognized exactly as each tilt.Our novel ROT had the potential to accurately indicate patient tilt during chest radiography, which could be helpful in terms of reproducibility and precise follow-up.Use of the ROT for determination of patient tilt can improve reproducibility in Fowler's position, allowing more accurate serial X-ray imaging.

BACKGROUND: Optimal neuromuscular performance is essential for futsal athletes and it has been linked to body composition. AIM: The present study aimed to verify the relationship between absolute (total amount; kg) and relative (percentage; %) lower-limb and thigh lean and fat mass with isometric and dynamic maximal strength in professional futsal players. METHOD: Sixteen male elite futsal athletes participated in this study. Unilateral lower-limb and thigh lean and fat mass were evaluated using DXA (Dual-Energy X-ray Absorptiometry) and strength performance using unilateral isokinetic concentric-eccentric knee extension (KE) and flexion (KF) peak torque (PT), KE and KF isometric PT at 30° and 70°, and hamstrings-to-quadriceps ratios (H:Q). RESULTS: Unilateral lower-limb lean mass amount showed a significant positive correlation with concentric and isometric KE PT (60°/s: r=0.58; 30°: r=0.55; 70°: r=0.65), and with concentric (60°/s: r=0.61), eccentric (60°/s: r=0.67) and isometric (30°:r=0.79; 70°: r=0.70) KF PT. No significant relationship between lower-limb lean and fat mass percentage with isokinetic performance was verified. Thigh lean mass amount had a significant positive correlation with concentric and isometric KE PT (60°/s:r=0.52; 30°: r=0.51; 70°: r=0.84) and also with concentric (60°/s: r=0.75) and eccentric KF PT (60°/s:r=0.54), while thigh fat mass amount was only associated with eccentric KF PT (r=0.54). No significant correlation between lower-limb and thigh body composition with H:Q ratios was observed. CONCLUSION: In conclusion, contrary to the relative (%) values, absolute (kg) lower-limb and thigh fat and lean mass present a significant correlation with dynamic and isometric KE and KF performance in futsal players.

El objetivo de este estudio fue analizar el valor de mercado (VM) de futbolistas profesionales de las 5 mejores ligas europeas de fútbol. Se analizó el efecto de las variables independientes sobre el VM de los futbolistas, mediante una ANOVA con un nivel de significación de p 30 años. Los delanteros y centrocampistas, mostraron mayor VM en comparación con los porteros y los laterales. Los futbolistas que representaron a sus países en partidos internacionales mostraron mayor VM en comparación con los que no lo hicieron. Los futbolistas de formación juvenil en clubes de la PL y La Liga (LL) mostraron mayor VM en comparación con los futbolistas de otros clubes. En conclusión, factores intrínsecos y extrínsecos afectan el VM de los futbolistas. Los resultados de esta investigación pueden ser útiles para los directores deportivos en el proceso de selección de futbolistas profesionales. The objective of this study was analysed to the market value (MV) of professional soccer players from Top-5 European Soccer Leagues. An ANOVA analysed the effect of the independent variables on the MV of the soccer players, with p30 years. Forward and midfielders showed greater MV compared to goalkeepers and backs. Soccer players that represented their countries at international matches showed greater MV compared to those that did not. The soccer players with a youth background formation in their clubs in the Premier League and La Liga showed greater MV compared to soccer players coming from other clubs. In conclusion, intrinsic and extrinsic factors affect the MV of soccer players. Research results may be useful to the sport managers in the selection of professional soccer players. O objetivo deste estudo foi analisar o valor de mercado (VM) de jogadores de futebol profissional das 5 melhores ligas europeias de futebol. O efeito das variáveis independentes sobre a VM dos futebolistas foi analisado por meio de uma ANOVA com nível de significância de p 30 anos. Os atacantes e meio-campistas mostraram maior VM em relação aos goleiros e laterais. Os jogadores de futebol que representaram seus países em jogos internacionais apresentaram maior MV em comparação com aqueles que não o fizeram. Jogadores de futebol em formação de jovens em clubes da PL e La Liga (LL) apresentaram maior VM em comparação com jogadores de futebol de outros clubes. Em conclusão, fatores intrínsecos e extrínsecos afetam a VM dos jogadores de futebol. Os resultados desta pesquisa podem ser úteis para dirigentes esportivos no processo de seleção de jogadores profissionais de futebol.

Based on the Riemann problem in compressible fluid dynamics, if the head of an unsteady jet acts as a physical piston for air compression, a higher-pressure field than that of the kinetic pressure from a steady jet can be generated. In this study, the pressure characteristics of this air compression method, referred to as “remote in-tube air compression,” were evaluated. The generated unsteady jet exhibited a high-pressure region in its central part that effectively acted as a physical piston (piston effect). Depending on the distance between the unsteady jet generator and a cylindrical test section, the overpressure inside the test section reached the maximum value when the cross section of the jet head and the test section were matched. This matching condition was consistent with the in-tube pressure characteristics, thereby yielding an effective method for the remote generation of a high-pressure region using a simple device.

Context Athletes' sleep is the most important recovery strategy and has received growing attention. However, athletes may experience sleep disruptions due to numerous factors, such as training and competition workloads, travel, changes in sleep-wake schedules, and sleeping environments. They often spend nights in unfamiliar hotels, and sharing a bed, room, or both with another person might affect sleep duration and quality. Objective To analyze the effect of sleeping in shared (SRs) versus individual (IRs) rooms on objective and subjective sleep and on slow-wave-sleep–derived cardiac autonomic activity during an official training camp in elite youth soccer players. Training and match workloads were characterized. Design Observational case study. Setting Hotel accommodations. Patients or Other Participants Thirteen elite male youth soccer players. Results Players slept longer in IRs than in SRs (+1:28 [95% CI = 1:18, 1:42] hours:minutes; P < .001). Sleep efficiency was higher in IRs than in SRs (+12% [95% CI = 10%, 15%]; P < .001), whereas sleep latency was shorter in IRs than in SRs (−3 [95% CI = −15, −4] minutes; P < .001). Subjective sleep quality was lower in IRs than in SRs (−2 [−3 to −2] arbitrary units; P < .001). No differences were found for slow-wave-sleep–derived cardiac autonomic activity or for training or match workloads between training camps. Conclusions During soccer training camps, sleep may be affected by whether the athlete is in an SR versus an IR.

International audience; The ground-state magnetic dipole moment of the neutron-rich O21 isotope has been measured via β-ray-detected nuclear magnetic resonance (β-NMR) spectroscopy by using a spin-polarized secondary beam of O21 produced from the Ne22 primary beam. From the present measurement, the g factor |gexp(Og.s.21)|=0.6036(14) has been determined. Based on the comparison of this value with Schmidt values, we unambiguously confirm the νd5/2 configuration with spin and parity assignments Iπ=5/2+ for the O21 ground state, suggested by previously reported studies. Consequently, the magnetic moment has been determined as μexp(Og.s.21)=(−)1.5090(35)μN. The obtained experimental magnetic moment is in good agreement with the predictions of the shell-model calculations using the USD, YSOX, and SDPF-M interactions as well as random phase approximation (RPA) calculations. This observation indicates that the O21 nucleus in its ground state does not manifest any anomalous structure and is not influenced by the proximity of the drip line.

This study aimed to examine the effects of quercetin glycoside-containing beverages on cognitive function and cerebral blood flow (CBF) in adult men and women aged between 60 and 75 years.Eighty healthy men and women with no cognitive impairment and aware of ageing-related forgetfulness underwent a placebo-controlled, randomized, double-blind, and parallel-group trial. They regularly consumed 500 mL of beverage containing 110 mg of quercetin glycoside as isoquercitrin for 40 weeks. Cognitive function assessment by Cognitrax was the endpoint of the study. The participants were assessed for CBF, health-related quality of life, as well as physical, biological, and hematological parameters, and lateral index.Cognitrax demonstrated that the reaction time significantly improved in the quercetin glycoside intake group. The CBF measurement suggested that quercetin glycoside intake could likely suppress the decrease in cerebral blood volume, CBF, and cerebral activity owing to stress alleviation and inhibition of the accumulation of amyloid β (Aβ), a waste product in the brain, although there were no significant differences between the groups.Quercetin glycoside intake as a beverage could improve reaction time and may potentially inhibit the decrease in CBF and suppress Aβ accumulation.

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Objectives The aim of this AFIRE (Atrial Fibrillation and Ischemic Events With Rivaroxaban in Patients With Stable Coronary Artery Disease) trial subgroup analysis was to examine rivaroxaban monotherapy benefits and their relation to the time between stenting and enrollment among patients after coronary stenting. Background Of 2,215 patients with atrial fibrillation and stable coronary artery disease in the AFIRE trial, rivaroxaban monotherapy was noninferior to rivaroxaban plus antiplatelet therapy (combination therapy) in terms of efficacy and superior for safety endpoints. However, thrombotic risk after antiplatelet therapy cessation remained a concern among 1,444 patients who had undergone coronary stenting >1 year before enrollment. Methods The benefits of rivaroxaban monotherapy in coronary stenting subgroups were assessed for efficacy (a composite of stroke, systemic embolism, myocardial infarction, unstable angina requiring revascularization, or death of any cause), safety (major bleeding defined according to International Society on Thrombosis and Haemostasis criteria), ischemic endpoints, net adverse clinical event, and time between stenting and enrollment. Results Efficacy and safety endpoints for monotherapy were superior to combination therapy, with HRs of 0.70 for efficacy (95% CI: 0.50-0.98; P = 0.036) and 0.55 for safety (95% CI: 0.33-0.92; P = 0.019). For ischemic endpoints, the HR was 0.82 (95% CI: 0.58-1.15; P = 0.240). The HR became smaller with longer time between stenting and enrollment (efficacy, P for interaction = 0.158; safety, P = 0.097). Conclusions In patients with atrial fibrillation after coronary stenting, the benefits of rivaroxaban monotherapy for efficacy and safety endpoints were consistent with those in the whole AFIRE trial population. The benefits became apparent with longer time between stenting and enrollment. (Atrial Fibrillation and Ischemic Events With Rivaroxaban in Patients With Stable Coronary Artery Disease Study [AFIRE]; UMIN000016612 , NCT02642419 )

Andes Large-area PArticle detector for Cosmic-ray physics and Astronomy (ALPACA) is an international experiment that applies southern very-high-energy (VHE) gamma-ray astronomy to determine the origin of cosmic rays around the knee energy region ($10^{15}\, {\rm eV} - 10^{16}\, {\rm eV}$). The experiment consists of an air shower (AS) array with a surface of $83, 000\, {\rm m}^2$ and an underground water Cherenkov muon detector (MD) array covering $5, 400\, {\rm m}^2$. The experimental site is at the Mt. Chacaltaya plateau in La Paz, Bolivia, with an altitude of $4, 740\, {\rm m}$ corresponding to $572\, {\rm g}/{\rm cm}^2$ atmospheric thickness. As the prototype experiment of ALPACA, the ALPAQUITA experiment aims to begin data acquisition in late 2021. The ALPAQUITA array consists of a smaller AS array ($18, 450\, {\rm m}^2$) and underground MD ($900\, {\rm m}^2$), which are now under construction. ALPAQUITA's sensitivity to gamma-ray sources is evaluated with Monte Carlo simulations. The simulation finds that five gamma-ray sources observed by H.E.S.S. and HAWC experiments will be detected by ALPAQUITA beyond 10 TeV and one out of these five - HESS J1702-420A - above 300 TeV in one calendar year observation. The latter finding means that scientific discussions can be made on the emission mechanism of gamma rays beyond 100 TeV from southern sources on the basis of the observational results of this prototype experiment., This is the manuscript accepted by Experimental Astronomy. For the published article, see https://doi.org/10.1007/s10686-021-09796-8

We conducted comprehensive clinical and molecular characterization of claudin 18.2 expression (CLDN18.2) in advanced gastric or gastroesophageal junction cancer (GC/GEJC).Patients with advanced GC/GEJC who received systemic chemotherapy from October 2015 to December 2019 with available tumor specimens were analyzed. We evaluated clinicopathological features of CLDN18.2 expression with four molecular subtypes: mismatch repair deficient, Epstein-Barr virus-positive, human epidermal growth factor receptor 2-positive, and others. In addition, programmed death-ligand 1 (PD-L1) combined positive score (CPS), genomic alterations, and the expression of immune cell markers were assessed. Clinical outcomes of standard first- or second-line chemotherapy and subsequent anti-programmed cell death protein 1 (anti-PD-1) therapy were also investigated according to CLDN18.2 expression.Among 408 patients, CLDN18.2-positive (moderate-to-strong expression in ≥75%) was identified in 98 patients (24.0%) with almost equal distribution in the four molecular subtypes or CPS subgroups. CLDN18.2-positive was associated with Borrmann type 4, KRAS amplification, low CD16, and high CD68 expression. Overall survival with first-line chemotherapy was not significantly different between CLDN18.2-positive and -negative groups [median 18.4 versus 20.1 months; hazard ratio 1.26 (95% confidence interval 0.89-1.78); P = 0.191] regardless of stratification by PD-L1 CPS ≥5. Progression-free survival and objective response rates of first- and second-line chemotherapy, and anti-PD-1 therapy also showed no significant differences according to CLDN18.2 status.CLDN18.2 expression in advanced GC/GEJC was associated with some clinical and molecular features but had no impact on treatment outcomes with chemotherapy or checkpoint inhibition. CLDN18.2-positive also had no impact on overall survival. This information could be useful to interpret the results from currently ongoing clinical trials of CLDN18.2-targeted therapies for advanced GC/GEJC and to consider a treatment strategy for CLDN18.2-positive GC/GEJC.

SUMMARYCancers often display immune escape, but the mechanisms and potential for reversibility are incompletely understood. Epigenetic dysregulation has been implicated in the immune escape of various cancer types. We have identified the epigenetic modifier SET and MYND-domain containing protein 3 (SMYD3) as a mediator of immune escape in human papilloma virus (HPV)- negative head and neck squamous cell carcinoma (HNSCC), an aggressive disease with poor prognosis and low response to immunotherapy with pembrolizumab, a programmed-death-1 (PD-1) targeting antibody. SMYD3 loss increased the sensitivity of HNSCC cancer cells to IFN-β, resulting in upregulation of type I IFN response and antigen presentation machinery genes. We found that SMYD3 regulates the transcription of Ubiquitin-Like PHD And RING Finger Domain- Containing Protein 1 (UHRF1), a key epigenetic reader of trimethylated lysine 9 on histone H3 (H3K9me3), which binds to H3K9me3-enriched promoters of key immune-related genes and silences their expression. SMYD3 further maintains the repression of immune-related genes through the deposition of H4K20me3 within the gene body regions of these genes. In an anti-PD-1 immune checkpoint resistant syngeneic mouse model of HPV-negative HNSCC, Smyd3 depletion induced influx of CD8+ T-cells, upregulated PD-L1 and MHC class I molecules, and increased sensitivity to anti-PD-1 therapy. SMYD3 overexpression was associated with decreased CD8 T-cell infiltration in tumor samples from patients with HPV-negative HNSCC, and was associated with poor response to pembrolizumab. Overall, these data highlight a previously unreported function of SMYD3 as a master epigenetic regulator of anti-tumor immune response in HPV-negative HNSCC and provide a rationale for translational approaches combining SMYD3 depletion strategies with checkpoint blockade to overcome anti-PD-1 resistance in this devastating disease.

Objectives As heart failure with concomitant sarcopenia has a poor prognosis, simple methods for evaluating the appendicular skeletal muscle mass index (ASMI) are required. Recently, a model incorporating anthropometric data and the sarcopenia index, that is, the ratio of serum creatinine to cystatin C (Cre/CysC), was developed to estimate the appendicular skeletal muscle mass. We hypothesized that this model would be superior to the previous model, which uses only anthropometric data to predict the prognosis. This study aimed to compare the prognostic value of low ASMI as defined by the biomarker and anthropometric models in patients with heart failure. Methods Among 847 patients, we estimated ASMI using an anthropometric model consisting of age, body weight, and height in 791 patients and a biomarker model that incorporates age, body weight, hemoglobin, and Cre/CysC in 562 patients. Patients were divided into low and non-low ASMI groups according to the ASMI estimated by each model, using the cut-off proposed by the Asian Working Group for Sarcopenia. The primary outcome was all-cause mortality. Results Overall, 53.4% and 39.1% of patients were diagnosed with low ASMI by anthropometric and biomarker models, respectively. The agreement of the diagnosis of low ASMI between the two models was poor, with a kappa coefficient of 0.56 (95% confidence interval: 0.49–0.63). Kaplan-Meier curves showed that a low ASMI was significantly associated with all-cause death in both models. However, this association was retained after adjustment for other covariates in the biomarker model (hazard ratio: 2.60, p=0.003), but not in the anthropometric model (hazard ratio: 0.70, p=0.257). Conclusions and implications Among patients hospitalized with heart failure, a low ASMI estimated using the biomarker model, but not the anthropometric model, was significantly associated with all-cause mortality. Funding Acknowledgement Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Japan Agency for Medical Research and Development

Observation techniques of high-energy gamma rays using air showers have remarkably progressed via the Tibet AS$\gamma$, HAWC, and LHAASO experiments. These observations have significantly contributed to gamma-ray astronomy in the northern sky's sub-Peta electron volts (PeV) region. Moreover, in the southern sky, the ALPACA experiment is underway at 4,740\,m altitude on the Chacaltaya plateau in Bolivia. This experiment estimates the gamma-ray flux from the difference between the number of on-source and off-source events by real data, utilizing the gamma-ray detection efficiency calculated through Monte Carlo simulations, which in turn depends on the hadronic interaction models. Even though the number of cosmic-ray background events can be experimentally estimated, this model dependence affects the estimation of gamma-ray detection efficiency. However, previous reports have assumed that the model dependence is negligible and have not included it in the error of gamma-ray flux estimation. Using ALPAQUITA, the prototype experiment of ALPACA, we quantitatively evaluated the model dependence on hadronic interaction models for the first time. We evaluate the model dependence on hadronic interactions as less than 3.6\,\% in the typical gamma-ray flux estimation performed by ALPAQUITA; this is negligible compared with other uncertainties such as energy scale uncertainty in the energy range from 6 to 300 TeV, which is dominated by the Monte Carlo statistics. This upper limit of 3.6\,\% model dependence is expected to apply to ALPACA.

Study question Does the duration of cryo-storage of vitrified embryos affect obstetric and perinatal outcomes? Summary answer Duration of cryo-storage with an open vitrification system did not affect obstetric and perinatal outcomes. What is known already Frozen-thawed blastocyst transfer has been performed widely with improvement of embryo culture conditions and cryopreservation techniques. Although blastocyst vitrification has become an essential method to improve clinical outcomes of IVF, there has been little study into the relationship between a long duration of cryo-storage in liquid nitrogen and obstetric and perinatal outcomes. Study design, size, duration This retrospective study was conducted at Kyono ART Clinic from January 2007 to December 2020. This study includes a total of 1053 singletons derived from 2461 frozen-thawed blastocyst transfers in 2461 patients. Steel’s multiple comparison test was performed for clinical and perinatal outcomes with cases of cryo-storage of less than 3 months as a control group. P Participants/materials, setting, methods The subjects are patients who underwent their first single frozen-thawed blastocyst transfer (FBT) with an open vitrification system. Females >40 years old at cryopreservation and those who underwent preimplantation genetic testing were excluded. According to cryo-storage duration, patients were grouped as follows: group A, 0-3 months (1255 cycles); group B, 3-6 months (1008 cycles); group C, 6-12 months (162 cycles), group D, 12-24 months (36 cycles). Main results and the role of chance Both the mean maternal age at blastocyst cryopreservation (A: 33.7±3.6, B: 34.1±3.5, C: 34.8±3.2, D: 35.1±3.3) and the mean maternal age at FBT (A: 33.8±3.6, B: 34.4±3.5, C: 35.5±3.1, D: 36.4±3.3) in groups B, C, and D were significantly higher compared to those in group A.. There was no significant difference in the survival rate after blastocyst thawing [A: 97.4% (1256/1290), B: 98.3% (1010/1027), C: 99.4% (163/164), D: 97.2% (35/36)]. The pregnancy rate in group C was significantly lower compared to group A [A: 60.0% (752/1254), B: 61.4% (619/1008), C: 48.1% (78/162), D: 63.9% (23/36)] The gestational age of group C was significantly lower compared to group A (A: 39.5±1.8, B: 39.4±1.8, C: 38.4±3.1, D: 39.8±1.6), but there were no significant differences in live birth weight or height. There were no significant differences in congenital abnormality rate [A: 1.3% (7/541), B: 2.5% (11/440), C: 1.8% (1/56), D: 0.0% (0/16)], placental abnormalities such as placenta previa [A: 1.3% (7/541), B: 1.6% (7/440), C: 1.8% (1/56), D: 0.0% (0/16)], perinatal abnormalities such as hypertensive disorders of pregnancy or gestational diabetes [A: 6.1% (33/541), B: 8.6% (38/440), C: 1.8% (1/56), D: 6.3% (1/16)] among the four groups. Limitations, reasons for caution We could not provide sufficient information on confounding factors such as smoking habits, and the sample size was too small for multivariate analysis. The safety of longer storage will need to be verified as this has not been clarified in this study. Wider implications of the findings The pregnancy rate in group C was significantly lower than that in group A, but the effect was small: 0.065, power of 0.78. Our data suggested that the duration of cryopreservation with an open vitrification system in liquid nitrogen did not affect obstetric or perinatal outcomes. Trial registration number not applicable

Study question Which improves sperm recovery rate (SRR) for men with non-obstructive azoospermia (NOA), microdissection TESE (m-TESE) or conventional TESE (c-TESE)? Summary answer SRR of c-TESE vs. m-TESE for NOA was not significant. We need to develop a safer, less-invasive technique with fewer complications and higher SRR. What is known already m-TESE has been performed for men with NOA since Schlegel P’s report (Hum Reprod, 1999). Seminiferous tubules containing many developing germ cells were likely to be larger and more opaque and the ability to find spermatozoa increased from 45% (10/22) to 63% (17/27) after introduction of the microdissection technique. However, Jarvis S et al. (Turek PG group, 2019) reported that at least one site revealed mature sperm in 24 (29.3%) of 82 men with prior failed micro TESE procedures, and sperm were more likely to be found in the testis periphery rather than centrally with FNA mapping. Study design, size, duration TESE was performed in 1071 azoospermic patients in my clinics; c-TESE was performed for all azoospermic patients between 1996 and 2005 in Sendai; c-TESE for OA and m-TESE for NOA was performed from 2006 in Sendai, and from 2012 in Takanawa. The patients were divided into four groups; 1) FSH≦9.2mIU/ml, Testicular volume(TV)≧15/ml (OA), 2) FSH≦9.2mIU/ml, TV < 15ml, 3) FSH>9.2mIU/ml, TV≧15ml, 4) FSH>9.2mIU/ml, TV < 15ml (NOA). Participants/materials, setting, methods c-TESE was performed in 178 azoospermic men (50, 12, 7, 109, respectively) in 1996-2005. c-TESE was performed in 181 patients (131, 26, 15, 9, respectively) and m-TESE was performed in 541 patients (56, 33, 35, 416, respectively) in 2006 -2020. SRR, pregnancy rate (PR), and birth rate (BR) in 1996-2005 were compared with those in 2006-2020. Main results and the role of chance Statistical analysis was performed by chi-square analysis. SRR in c-TESE was 96.0% (48/50) in group 1 (OA) vs. 36.7% (40/109) in group 4 (NOA) in Sendai between 1996 and 2005 (P < 0.001). SRR of c-TESE was 36.7% (40/109) in group 4 (NOA, in Sendai, 1996-2006), whereas SRR of m-TESE was 31.3% (130/416) in group 4 (NOA, in Sendai and Takanawa, 2006-2020). There was no significant difference (np). Total SRR of conventional TESE for all azoospermic patients between 1996 and 2005 was 54.9% (96/175), whereas total SRR of c-TESE for OA and m-TESE for NOA between 2006 and 2020 was 51.7% (373/721). NOA rate 4) of azoospermic patients of 1996-2005 and that of 2006-2020 was 61.2% (109/178) and 58.9% (425/721), respectively. There was no significant difference (np). PR was 37.4% (61/163) and BR was 31.9% (52/263) in c-TESE (1996-2005), PR was 39.6% (388/980), and BR was 27.6% (270//980) in both c-TESE and m-TESE (2006–2020). There was a significant difference in birth rate (P < 0.05). In conclusion, SRR, PR, and BR were not improved even after m-TESE was performed. Considering the fact that m-TESE cannot find sperm thoroughly, adopting other techniques such as sperm fine-needle aspiration may be required. Limitations, reasons for caution Not applicable. Wider implications of the findings We could not show the effectiveness of m-TESE compared with c-TESE. However, the total volume of seminiferous tubules collected by c-TESE was the higher. The new one-step fine-needle aspiration has the potential to achieve higher sperm yield with fewer complications. Further advanced technology will hopefully improve the SRR in NOA. Trial registration number none

Purpose: The optimal power load is defined as the load that maximizes power output in a given exercise. This load can be determined through the use of various instruments, under different testing protocols. Specifically, the "optimum power load" (OPL) is derived from the load-velocity relationship, using only bar force and bar velocity in the power computation. The OPL is easily assessed using a simple incremental testing protocol, based on relative percentages of body mass. To date, several studies have examined the associations between the OPL and different sport-specific measures, as well as its acute and chronic effects on athletic performance. The aim of this brief review is to present and summarize the current evidence regarding the OPL, highlighting the main lines of research on this topic and discussing the potential applications of this novel approach for testing and training. Conclusions: The validity and simplicity of OPL-based schemes provide strong support for their use as an alternative to more traditional strength-power training strategies. The OPL method can be effectively used by coaches and sport scientists in different sports and populations, with different purposes and configurations. Sin financiación 4.211 JCR (2021) Q1, 20/88 Sport Sciences 1.623 SJR (2021) Q1, 11/294 Orthopedics and Sports Medicine No data IDR 2021 UEM

Intracellular Ca2+ plays pivotal roles in cardiac contraction by mediating excitation-contraction (EC) coupling and progression of hypertrophy in the heart. Ample evidence suggests that mechanism of EC coupling in immature hearts are different from those in the adults because of the structural immaturity of the sarcoplasmic reticulum (SR) intracellular Ca2+ store and the different expression of Ca2+-regulatory proteins. However, the detailed molecular mechanism is not completely understood. In the present study, we identified neuronal Ca2+ sensor-1 (NCS-1), an EF-hand Ca2+ binding protein that is important for neuronal functions, also functions as a novel regulator of EC coupling in young hearts. We found that NCS-1 is highly expressed in immature hearts, and its deletion decreased their contractile functions as well as intracellular Ca2+ signals. NCS-1 enhances Ca2+ signals mainly by promoting the Inositol 1,4,5-Trisphosphate receptor (IP3R) function, followed by Ca2+/Calmodulin-dependent Protein Kinase II (CaMKII) signaling, which results in a large increase in the SR Ca2+ content that enhances SR-dependent EC coupling. In addition, NCS-1 expression increases in the early stages of hypertrophy and promotes progression of hypertrophy at least in part through IP3R-dependent elevation of nuclear Ca2+ signaling. Our results reveal a previously unrecognized mechanism of EC coupling in young heart and the progression of cardiac hypertrophy. Furthermore, we found that NCS-1 contributes to stress tolerance in cardiomyocytes via activation of mitochondrial detoxification pathways. We propose that the proteins involved in NCS-1-mediated Ca2+ signaling can be novel therapeutic targets for cardiac diseases, especially in immature hearts.

On November 13, 1945, Honolulu residents awoke to news of a mass riot the previous evening by over one thousand sailors in the Damon Tract area in Honolulu. Although it was one of the largest postwar military uprisings on American soil, the riot itself has not been carefully examined in the historical record due other events and interests locally and nationally, as the media continued to operate within a highly militarized state. Remembering and understanding the Damon Tract riot became secondary to America’s Cold War interests in the Pacific, the growth of tourism in the Islands, and efforts to garner statehood for Hawai‘i that depended on unifying these historically contentious identities at the expense of acknowledging conflict that existed in the past.