1. Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch
- Author
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Xingwang Gu, Fan Zhou, and Junsheng Mu
- Subjects
Pluripotent Stem Cells ,Heart Diseases ,digestive, oral, and skin physiology ,Cell Culture Techniques ,Cell Differentiation ,General Medicine ,Biology ,Phenotype ,Cell biology ,Engineering ,Chemical stimuli ,In vivo ,Humans ,Myocytes, Cardiac ,Stress, Mechanical ,Stem cell ,Mechanical Processes ,Induced pluripotent stem cell ,Review Articles ,health care economics and organizations - Abstract
Stem cells have significant potential use in tissue regeneration, especially for treating cardiac diseases because of their multi-directional differentiation capability. By mimicking the in vivo physiological environment of native cardiomyocytes during their development and maturation, researchers have been able to induce pluripotent stem cell-derived cardiomyocytes (PSC-CMs) at high purity. However, the phenotype of these PSC-CMs is immature compared with that of adult cardiomyocytes. Various strategies have been explored to improve the maturity of PSC-CMs, such as long-term culturing, mechanical stimuli, chemical stimuli, and combinations of these strategies. Among these strategies, mechanical stretch as a key mechanical stimulus plays an important role in PSC-CM maturation. In this review, the optimal parameters of mechanical stretch, the effects of mechanical stretch on maturation of PSC-CMs, underlying molecular mechanisms as well as existing problems are discussed. Mechanical stretch is a powerful approach to promote the maturation of SC-CMs in terms of morphology, structure, and functionality. Nonetheless, further research efforts are needed to reach a satisfactory standard for clinical applications of PSC-CMs in treating cardiac diseases.
- Published
- 2021