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SummaryThe appropriate development of macrophages, the body’s professional phagocyte, is essential for organismal development, especially in mammals. This dependence is exemplified by the observation that loss-of-function mutations in colony stimulating factor 1 receptor (CSF1R) results in multiple tissue abnormalities owing to an absence of macrophages. Despite this importance, little is known about the molecular and cell biological regulation of macrophage development. Here, we report the surprising finding that the chloride-sensing kinase With-no-lysine 1 (WNK1) is required for development of tissue-resident macrophages (TRMs). Myeloid-specific deletion ofWnk1resulted in a dramatic loss of TRMs, disrupted organ development, systemic neutrophilia, and mortality between 3 and 4 weeks of age. Strikingly, we found that myeloid progenitors or precursors lacking WNK1 not only failed to differentiate into macrophages, but instead differentiated into neutrophils. Mechanistically, the cognate CSF1R cytokine macrophage-colony stimulating factor (M-CSF) stimulates macropinocytosis by both mouse and human myeloid progenitors and precursor cells. Macropinocytosis, in turn, induces chloride flux and WNK1 phosphorylation. Importantly, blocking macropinocytosis, perturbing chloride flux during macropinocytosis, and inhibiting WNK1 chloride-sensing activity each skewed myeloid progenitor differentiation from macrophages into neutrophils. Thus, we have elucidated a role for WNK1 during macropinocytosis and discovered a novel function of macropinocytosis in myeloid progenitors and precursor cells to ensure macrophage lineage fidelity.Highlights-Myeloid-specific WNK1 loss causes failed macrophage development and premature death-M-CSF-stimulated myeloid progenitors and precursors become neutrophils instead of macrophages-M-CSF induces macropinocytosis by myeloid progenitors, which depends on WNK1-Macropinocytosis enforces macrophage lineage commitment

IntroductionLiver hepatocellular carcinoma (LIHC), one of the most common malignancies worldwide, occurs with high incidence and mortality. Ferroptosis and necroptosis are critically associated with LIHC prognosis. Some long non-coding RNAs (lncRNAs) have been found to induce ferroptosis and necroptosis in hepatocellular carcinoma cells.MethodsCox regression analysis was used to construct a risk model for LIHC based on differentially expressed ferroptosis and necroptosis related lncRNAs (F-NLRs), and their expression in SMMC7721, HepG2 and WRL68 cells was detected by qPCR.ResultsFive F-NLRs were associated with LIHC prognosis, including KDM4A-AS1, ZFPM2-AS1, AC099850.3, MKLN1-AS, and BACE1-AS. Kaplan-Meier survival analysis indicated that patients with LIHC in the high-risk group were associated with poor prognosis. The combined F-NLR signature model demonstrated a prognostic AUC value of 0.789 and was more accurate than standard clinical variables for predicting LIHC prognosis. T cell functions and immunotherapy responses differed significantly between patients in the low- and high-risk groups. Additionally, immune checkpoints and m6A-related genes were differentially expressed between patients in the two risk groups. Furthermore, proteins encoded by the five F-NLRs were overexpressed in four liver cancer cell lines compared to that in human liver cell line WRL68. Pan-cancer examination revealed that expression levels of the five F-NLRs differed between most common tumor types and normal tissues.ConclusionF-NLRs identified in this study provide a predictive signature representing ferroptosis and necroptosis in LIHC, which correlated well with patient prognosis, clinicopathological characteristics, and immunotherapy responses. The study findings help to elucidate the mechanisms of F-NLRs in LIHC and provide further guidance for the selection and development of immunotherapeutic agents for LIHC.

Supplemental material, sj-pdf-1-imr-10.1177_03000605221147434 for The inflammatory cytokine profiles and ocular biometric characteristics of primary angle-closure glaucoma by Hong-Bo Zeng, Jun-Di Jiang, Rong Li, Jian Li, Wen-Juan Zeng, Xiong-Kai Li, Bin Hu, Fei Zhou, Hai-Ying Yu, Jian-Guo Xie, Zhi-Neng Ning and Xian Wang in Journal of International Medical Research

In task-oriented dialogue systems, Dialogue State Tracking (DST) aims to extract users' intentions from the dialogue history. Currently, most existing approaches suffer from error propagation and are unable to dynamically select relevant information when utilizing previous dialogue states. Moreover, the relations between the updates of different slots provide vital clues for DST. However, the existing approaches rely only on predefined graphs to indirectly capture the relations. In this paper, we propose a Dialogue State Distillation Network (DSDN) to utilize relevant information of previous dialogue states and migrate the gap of utilization between training and testing. Thus, it can dynamically exploit previous dialogue states and avoid introducing error propagation simultaneously. Further, we propose an inter-slot contrastive learning loss to effectively capture the slot co-update relations from dialogue context. Experiments are conducted on the widely used MultiWOZ 2.0 and MultiWOZ 2.1 datasets. The experimental results show that our proposed model achieves the state-of-the-art performance for DST., Comment: Accepted by AAAI 2023

Supplemental material, sj-pdf-1-imr-10.1177_03000605221147434 for The inflammatory cytokine profiles and ocular biometric characteristics of primary angle-closure glaucoma by Hong-Bo Zeng, Jun-Di Jiang, Rong Li, Jian Li, Wen-Juan Zeng, Xiong-Kai Li, Bin Hu, Fei Zhou, Hai-Ying Yu, Jian-Guo Xie, Zhi-Neng Ning and Xian Wang in Journal of International Medical Research

Supplemental material, sj-pdf-2-imr-10.1177_03000605221147434 for The inflammatory cytokine profiles and ocular biometric characteristics of primary angle-closure glaucoma by Hong-Bo Zeng, Jun-Di Jiang, Rong Li, Jian Li, Wen-Juan Zeng, Xiong-Kai Li, Bin Hu, Fei Zhou, Hai-Ying Yu, Jian-Guo Xie, Zhi-Neng Ning and Xian Wang in Journal of International Medical Research

Additional file 1: Table S1. The clinical information of HCC samples in the TCGA dataset and the ICGC dataset.

Supplemental material, sj-pdf-2-imr-10.1177_03000605221147434 for The inflammatory cytokine profiles and ocular biometric characteristics of primary angle-closure glaucoma by Hong-Bo Zeng, Jun-Di Jiang, Rong Li, Jian Li, Wen-Juan Zeng, Xiong-Kai Li, Bin Hu, Fei Zhou, Hai-Ying Yu, Jian-Guo Xie, Zhi-Neng Ning and Xian Wang in Journal of International Medical Research

Additional file 2: Table S2. List of primers.

Recently, spoken dialogue systems have been widely deployed in a variety of applications, serving a huge number of end-users. A common issue is that the errors resulting from noisy utterances, semantic misunderstandings, or lack of knowledge make it hard for a real system to respond properly, possibly leading to an unsatisfactory user experience. To avoid such a case, we consider a proactive interaction mechanism where the system predicts the user satisfaction with the candidate response before giving it to the user. If the user is not likely to be satisfied according to the prediction, the system will ask the user a suitable question to determine the real intent of the user instead of providing the response directly. With such an interaction with the user, the system can give a better response to the user. Previous models that predict the user satisfaction are not applicable to DuerOS which is a large-scale commercial dialogue system. They are based on hand-crafted features and thus can hardly learn the complex patterns lying behind millions of conversations and temporal dependency in multiple turns of the conversation. Moreover, they are trained and evaluated on the benchmark datasets with adequate labels, which are expensive to obtain in a commercial dialogue system. To face these challenges, we propose a pipeline to predict the user satisfaction to help DuerOS decide whether to ask for clarification in each turn. Specifically, we propose to first generate a large number of weak labels and then train a transformer-based model to predict the user satisfaction with these weak labels. Empirically, we deploy and evaluate our model on DuerOS, and observe a 19% relative improvement on the accuracy of user satisfaction prediction and 2.3% relative improvement on user experience., Comment: Accepted by CIKM-22

Let $R_n$ be the number of distinct values of the $n$ simple samples from an infinite discrete distribution. In 1960 Bahadure proved $\displaystyle \lim_{n\to \infty} \frac{R_n}{\Enum R_n}=1$ in probability; Chen et al. proved the limit in the sense of almost sure convergence, along with other results. In this note we present results of CLT, MDP and LDP for $R_n$ under mild conditions., Comment: 12 pages

Additional file 1: Table S1. Gene primer sequences for q-PCR. Fig. S1. Diameter distribution of electrospun fibers of A20 and R20 groups. Fig. S2. (A & B) Immunofluorescence staining of iNOS at days 7 and 14. (C & D) Mean fluorescence intensity of iNOS in each group of skin sections on days 7 and 14. Nucleus was stained blue and iNOS was stained red. Scale bar = 200 μm. Fig. S3. CCK-8 assay of MAEC culturing with macrophage conditioned medium. (*p < 0.05, n=3). Fig. S4. Migration of MAEC under conditional medium. Scale bar = 500 μm. Fig. S5. The expression of angiogenesis related genes of MAEC cultured with macrophage conditioned medium. (*p < 0.05, **p < 0.01, n = 3)

Background Inflammation is integral to the pathophysiology of ischemic stroke and a prime target for the development of new stroke therapies. The aim of the present study is to seek out the regulatory mechanism of circCDC14A in neuroinflammatory injury in tMCAO mice. Methods The expression level of circCDC14A in peri-infarct cortex and plasma of mice were detected by qPCR. The localization of circCDC14A in peripheral blood cells and peri-infarct cortex of tMCAO mice were explored by in situ hybridization and immunofluorescence colocalization staining. Lentivirus were microinjected into lateral ventricular of brain or injected into tail vein to interfere with the expression of circCDC14A, thus their effects on behavior, morphology, and molecular biology of tMCAO mice were analyzed. Results The expression of circCDC14A in plasma and peri-infarct cortex of tMCAO mice significantly increased, and circCDC14A was mainly localized in neutrophils peripherally while in astrocytes in peri-infarct cortex centrally. Tail vein injection of lentivirus to interfere with the expression of circCDC14A significantly reduced the infarct volume (P P P P P P Conclusion Knocking down the expression of circCDC14A in peripheral blood cells relieved astrocytes activation in peri-infarct cortex, thereby relieved brain damage in the acute phase of ischemic stroke.

Jian-Hui Xie, Hai-Bo Mei, Ya-Qi Ouyang, Yuan Huang, Kun Liu, Guang-Hui Zhu, Shu-Ting Hou, Lan-Ying Wang Department of Orthopedics, Hunan Children’s Hospital, Changsha, 410007, People’s Republic of ChinaCorrespondence: Hai-Bo Mei, Department of Orthopedics, Hunan Children’s Hospital, No. 86 of Ziyuan Street, Yuhua District, Changsha, 410007, People’s Republic of China, Tel +86 139 7519 7731, Fax +86 731-85356666, Email mei_haibo@yeah.netAim: To investigate the status of the family resilience score of caregivers of children with congenital pseudarthrosis of the tibia (CPT) and analyse its influencing factors to provide a reference and basis for clinical formulation of a family resilience intervention programme.Methods: A total of 379 families of children with CPT were included in this study, which used a combination questionnaire to investigate the families of children with CPT. The researchers sent a link to the questionnaire to all eligible subjects to conduct a survey on the characteristics of families of children with CPT. Single factor analysis was used to compare the total scores of family resilience. Multiple linear regression was used to determine meaningful variables for single factor analysis.Results: The average family resilience score was 151.79 ± 30.79, the lowest score was 20 and the highest score was 231. The CPT usually occurred in unilateral limbs (37.63%). The payment methods of medical expenses were through a rural cooperative medical system (44.85%). The caregivers were mostly the mothers (62.80%) and caregivers were 31– 40 years old (62.27%). Most caregivers were farmers (30.34%). The average monthly income of the families in the past year was 1000– 5000 yuan (54.09%). There were significant differences in family resilience scores between caregivers’ working conditions (P = 0.039) and average monthly incomes in the past year (P = 0.008). The working conditions of caregivers and the average monthly income of families in the past year were the main factors affecting the family resilience of caregivers of children with CPT.Conclusion: The caregivers’ working conditions and the average monthly income of families were the influencing factors in the family resilience score.Keywords: congenital pseudarthrosis of the tibia, family resilience, family characteristics, influencing factor

BackgroundTakayasu’s arteritis (TA) is a type of primary large vessel vasculitis. Th1, Th17, and Tfh cells have been reported to be associated with TA relapse. However, the relationship between regulatory T cells (Tregs) and TA remains unclear.ObjectiveTo analyze the levels of circulating lymphocytes, especially Treg cells (CD4+CD25+FOXP3+ T cells) and serum cytokines in TA patients and explore their relationship with their changes and TA disease activity.MethodsA total of 57 TA patients and 43 sex- and age-matched healthy controls (HCs) were enrolled. According to NIH standards, 36 patients had active disease status. Flow cytometry combined with counting was used to detect the absolute numbers and ratios of Th1, Th2, Th17, and Treg cells in the peripheral blood of all the subjects. Magnetic bead-based multiplex immunoassay was used to detect cytokines.ResultsCompared to HCs, the absolute number and proportion of peripheral Treg cells in TA patients was significantly decreased, while Th17 cells were significantly increased. Furthermore, compared to the inactive group, the TA active group had significantly increased levels of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α, but lower IL-10 levels. The absolute number of Th2 cells was negatively associated with platelet (PLT) and NIS scores in TA patients. The proportion of Th2 cells was negatively associated with the erythrocyte sedimentation rate in TA patients. After treatment, Treg cells were markedly increased.ConclusionThere was a Th17-Treg cell imbalance with a significant reduction in peripheral Treg cells and an increase in Th17 cells in TA patients compared to the HCs. The levels of IL-6, IL-10, IL-17, and TNF-α appeared to be related to disease activity.

The column buckling performance of steel circular columns fabricated by submerged arc welding, high-frequency welding and hot-rolled forming was investigated. Nine axially compressed columnar speci...

The issue of trust has increasingly attracted attention in the business ethics literature. Our aim is to contribute further to this literature by examining how the use of the carbon copy (cc) function in email communication influences felt trust. We develop the argument that the use of cc enhances transparency—representing an important characteristic of workplace ethics—and hence promotes trust. We further argue that a downside of the cc option may be that it can also be experienced as a control mechanism, which may therefore negatively affect trust. The results of our first study showed that the use of cc indeed enhances perceived transparency, but at the same time also leads to the experience of increased control. Building upon this insight, the findings of five subsequent studies consistently revealed that the use of cc negatively influences felt trust. More precisely, employees felt trusted the least when the supervisor was always included in cc (Studies 2 and 3). This effect on felt trust also negatively influenced how trustworthy the organizational climate was perceived (Study 4). We further replicated these results in two field surveys, which showed that the negative effect of cc on felt trust lowered perceptions of psychological safety (Study 5) and contributed to a culture of fear (Study 6). Taken together, our findings suggest that when transparency in email communications is experienced as a control mechanism, its use is perceived as unethical, rather than as ethical. Implications and recommendations for future business ethics research are discussed.

A finite-element method (FEM) model was used to numerically investigate the overall buckling behaviours of hot-rolled (HR), submerged arc welded (SAW) and high-frequency welded (HFW) steel circular columns under axial compression. A three-dimensional FEM model of circular hollow sections was developed by using shell elements considering material non-linearities, geometric imperfections and residual stress. The FEM model was used to simulate experimental studies carried out by past researchers. Good agreement was found between the numerical and experimental results, which validate the FEM model for further research. The validated FEM model was used to analyse 180 numerically generated HR, SAW and HFW steel circular columns with various section sizes and slenderness values. The numerical results were then compared with existing column design curves in Chinese, European and American codes. To further improve the design efficiency, new column design curves for steel circular columns are recommended ba...

Task-oriented dialogue systems have been plagued by the difficulties of obtaining large-scale and high-quality annotated conversations. Furthermore, most of the publicly available datasets only include written conversations, which are insufficient to reflect actual human behaviors in practical spoken dialogue systems. In this paper, we propose Task-oriented Dialogue Data Augmentation (TOD-DA), a novel model-agnostic data augmentation paradigm to boost the robustness of task-oriented dialogue modeling on spoken conversations. The TOD-DA consists of two modules: 1) Dialogue Enrichment to expand training data on task-oriented conversations for easing data sparsity and 2) Spoken Conversation Simulator to imitate oral style expressions and speech recognition errors in diverse granularities for bridging the gap between written and spoken conversations. With such designs, our approach ranked first in both tasks of DSTC10 Track2, a benchmark for task-oriented dialogue modeling on spoken conversations, demonstrating the superiority and effectiveness of our proposed TOD-DA., Comment: Accepted to the AAAI-22 DSTC10 Workshop. First three authors contributed equally to this work

Additional file 1.

Additional file 1: Fig. S1. Soil water content. Statistical significance was identified by the Wilcoxon test with false discovery rate (FDR)-corrected pairwise P values. *, P

Search strategy for the Cochrane database. Table S2 Search strategy for other databases. (DOCX 63 kb)

This article explores the possibility of disaggregating query/question information in e-learning system online lectures or course recommendations. Information arrangement includes reading, parsing and classification of inquiry/question messages. Data extraction is a kind of shallow content processing. It finds a set of predefined applicable content in the feature language archives and performs common language processing through artificial intelligence strategies. During online lectures, many problems emerged in the listener’s minds, and the development of query optimization systems is of great significance to the evaluation of problems in online lectures. The results shows that our proposed method improve the classification of action verbs to a more accurate level. Later, we evaluated our proposed method, and measured a very high macro average for all one-sixth of the cognitive domain. We also provide the analytical examination in which we compared the designed method with the state of the art methods. The results indicate that the proposed method outperform the traditional methods

Additional file 1: Table S1. Involved experimental mice in tMCAO modeling. Figure S1. Gene information and organ distribution in mice of circCDC14A. Figure S2. Colocalization of circCDC14A with neuron in peri-infarct cortex of sham-operated and tMCAO mice from 1 day up to 7 days after modeling. Figure S3. Colocalization of circCDC14A with microglia in peri-infarct cortex of sham-operated and tMCAO mice from 1 day up to 7 days after modeling. Figure S4. Relative expression of circCDC14A in OGD/R treated primary astrocytes. Figure S5. The effect of knocking down circCDC14A peripherally on mRNA level of CDC14A in peripheral WBC and peri-infarct cortex. Figure S6. The effect of knocking down circCDC14A peripherally on neuroinflammation state of microglia and cytokines in tMCAO mice.

BACKGROUND: Cervical adenoid cystic carcinoma (ACC) and adenoid basal carcinoma (ABC) are rare cervical cancer types and have unclarified clinicopathological features and survival outcomes. This retrospective study focused on predicting the value of radiotherapy or/and chemotherapy for cervical ACC and ABC patients. METHODS: The clinical data of cervical ACC and ABC patients in the Surveillance, Epidemiology, and End Results (SEER) database from 1973 and 2013 were included. The clinicopathological features, Kaplan-Meier curves, and overall survival (OS) of patients were evaluated. The prognostic nomogram was established based on the multivariate Cox models. To validate the nomogram prediction, Harrell’s Concordance index (C-index) was calculated and receiver operating characteristic (ROC) curves were generated. RESULTS: A total of 84 cervical ACC and 82 ABC patients were identified, and ABC patients had better 10-year OS than ACC patients (60.81% vs. 36.94%, P=0.001). Age, ACC, surgery, radiotherapy, chemotherapy, and regional node involvement were significantly correlated with patient prognosis. In the multivariate analysis, only age >80 years (HR =5.945, 95% CI: 1.912–18.485, P=0.002) and age 70–80 years (HR =4.803, 95% CI: 1.626–14.188, P=0.005) were independent predictors of patient prognosis. In subgroup analysis, patients who underwent surgery (HR =2.199, 95% CI: 1.085–4.455, P=0.029) and the ABC subgroup (HR =4.233, 95% CI: 1.532–11.696, P=0.005) received radiotherapy, chemotherapy, or chemoradiotherapy with a poor prognosis. Patients received radiotherapy (HR =1.936, 95% CI: 1.208–3.105, P=0.006) was associated with a poor prognosis, while surgical patients had a better prognosis (HR =0.535, 95% CI: 0.344–0.832, P=0.006). CONCLUSIONS: Cervical ABC patients had a better survival time than cervical ACC patients. We found that increased age was potentially an independent risk factor for poor prognosis, surgical patients had a better prognosis, and radiotherapy, or chemotherapy combination treatment had an unfavorable tendency to prognosis.

The errors-in-variables (EIV) model is applied to surveying and mapping fields such as empirical coordinate transformation, line/plane fitting and rigorous modelling of point clouds and so on as it takes the errors both in coefficient matrix and observation vector into account. In many cases, not all of the elements in coefficient matrix are random or some of the elements are functionally dependent. The partial EIV (PEIV) model is more suitable in dealing with such structured coefficient matrix. Furthermore, when some reliable prior information expressed by inequality constraints is considered, the adjustment result of inequality constrained PEIV (ICPEIV) model is expected to be improved. There are two kinds of algorithms to solve the ICPEIV model under the weighted total least squares (WTLS) criterion currently. On the one hand, one can linearize the PEIV model and transform it into a sequence of quadratic programming (QP) sub-problems. On the other hand, one can directly solve the nonlinear target function by common used programming algorithms.All the QP algorithms and nonlinear programming methods are complicated and not familiar to the geodesists, so the ICPEIV model is not widely used in geodesy. In this contribution, an algorithm based on standard least squares is proposed. First, the estimation of model parameters and random variables in coefficient matrix are separated according to the Karush-Kuhn-Tucker (KKT) conditions of the minimization problem. The model parameters are obtained by solving the QP sub-problems while the variables are determined by the functional relationship between them. Then the QP problem is transformed to a system of linear equations with nonnegative Lagrange multipliers which is solved by an improved Jacobi iterative algorithm. It is similar to the equality-constrained least squares problem. The algorithm is simple because the linearization process is not required and it has the same form of classical least squares adjustment. Finally, two empirical examples are presented. The linear approximation algorithm, the sequential quadratic programming algorithm and the standard least squares algorithm are used. The examples show that the new method is efficient in computation and easy to implement, so it is a beneficial extension of classical least squares theory.

Additional file 10: Figure S5. The relationship between BATF2 and p53. a The online tool STRING was used to predict potential protein-protein interactions. b Representative images of p53 IHC staining in xenograft samples. c Wound healing assays showed that the effect of BATF2 overexpression on HGC-27 cell migration was rescued by p53 siRNA transfection. Representative images are presented (*P

Additional file 1. Supplementary Materials and Methods.

Additional file 7: Figure S2. The effect of BATF2 expression on GC cell proliferation. a MGC-803 and HGC-27 cells with stable BATF2 overexpression or knockdown were created. The changes in BATF2 expression were confirmed using western blotting. b Colony formation and CCK-8 assays with stably transfected MGC-803 cells were performed. Representative images and quantification of the results are presented (**P

Additional file 2: Figure S1. Selection of the study population. Figure S2. Kaplan-Meier analysis of overall survival according to (A) the preoperative PLR, (B) the preoperative LMR, (C) the preoperative hemoglobin level, (D) the combination of the preoperative serum hemoglobin level and the LMR. Figure S3. Time-dependent ROC curves of the CBCS, mGPS, and CRP/Alb for the prediction of overall survival. The dotted lines in Fig. 2 represent the 95% CI, and the unit of time is months.

Additional file 5: Table S3. Analysis of the recurrence sites of GC after curative gastrectomy

Additional file 3: Figure S1. The expression and prognostic value of BATF2 in GC. a The mRNA levels of BATF2 in human normal gastric epithelial cells (GES-1) and human GC cell lines (HGC-27, MGC-803, AGS, MKN-45 and SNU-216) were detected by real-time PCR and quantified (***P

Additional file 8: Figure S3. The effect of BATF2 expression on GC cell invasion and migration. a-b Transwell and wound healing assays with stably transfected MGC-803 cells were performed. Representative images and quantification of the results are presented (**P

Additional file 5 : Supplemental Figure 2. The cutoff points of the skeletal muscle index (SMI) for sarcopenia defined by X-tile software. (A) X-tile plots for males (44.3 cm2/m2, χ2 = 4.2611, p = 0.038) and (B) females (32.4 cm2/m2, χ2 = 1.0039, p = 0.214) are shown.

Additional file 1 Table S1. Reference ranges of peripheral blood lymphocyte subsets in 150 healthy adults. Table S2. Comparison of peripheral blood lymphocyte subset reference ranges from the present study with those published previously. Table S3. Distribution and reference ranges of lymphocyte subsets based on age and sex.

Additional file 2: Table S1. Primers used for qRT-PCR.

Additional file 2 : Supplemental Table 2. Univariate and multivariate analyses of factors associated with 3-year overall survival (OS) and recurrence-free survival (RFS) rates in patients with gNEC.

Additional file 9: Figure S4. The clinical value of BATF2 depends on ERK activity. a The expression of critical members of the MAPK pathway in stably transfected MGC-803 cells was examined by western blotting. b Wound healing assays showed that the effect of BATF2 downregulation on SNU-216 cell migration was rescued by U0126 treatment. Representative images are presented (**P

Additional file 4 : Supplemental Figure 1. Computed tomography (CT) image captured at the third lumbar vertebral (L3) level. The following skeletal muscles are outlined in red: rectus abdominis; psoas, quadratus lumborum, paraspinal, transverse abdominal, external oblique, internal oblique, and rectus abdominis muscles. This male patient with sarcopenia had an L3 muscle index of 52.35 cm2/m2.

Additional file 1 : Supplemental Table 1. Clinicopathological characteristics of patients treated at FMUUH and FMUFAH.

Additional file 1: Table S1. Clinicopathological characteristics. Table S2. Comparison of the AUCs between CBC-based parameters. Table S3. Definition of the complete blood count-based score (CBCS).

Additional file 4: Table S2. Univariate and multivariate analyses of overall survival for GC patients

Supplemental_material for The Diagnostic Value of Serum L1CAM in Patients With Colorectal Cancer by Ling-Yu Chu, Dong-Ming Guo, Jun-Tian Chen, Wang-Kai Fang, Jian-Jun Xie, Yu-Hui Peng, Yi-Wei Xu and Xin-Xin Li in Technology in Cancer Research & Treatment

Additional file 11: Figure S6. Relative expression of BATF2 and METTL3 in GC shown by H. pylori status. a BATF2 and METTL3 IHC scores from the internal cohort. b Relative expression of BATF2 and METTL3 from the ACRG dataset (GSE62254). ns: no significant difference.

The Fiber Bragg Grating (FBG) sensor was embedded in cement mortar after it was nickel electroplating protected. The stresses on the embedded FBG under varied temperature were analyzed. The temperature sensitivity of embedded FBG was achieved in theory base on stress analysis and verified by experiment. The temperature sensitivity of embedded FBG is 19.2188 pm/°C in theory, while the experiment result is 20.688 pm/°C. A finite element analysis software (ANSYS) was used to simulate the stresses on the embedded FBG. The results of theoretical analysis match the ones from experiments and simulations very well.