13 results on '"Xiaofen Xiao"'
Search Results
2. Ultrasensitive detection of DNA methyltransferase activity: a novel dual-amplification fluorescence technique
- Author
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Qilin Wen, Dandan Li, Guidan Huang, Huai Xi, Hongcheng Pan, Lianming Zhang, Ziyuan Li, Xiaofen Xiao, and Wenyuan Zhu
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Spectrometry, Fluorescence ,Electrochemistry ,Environmental Chemistry ,Methyltransferases ,DNA ,Biosensing Techniques ,DNA Methylation ,Biochemistry ,DNA Modification Methylases ,Spectroscopy ,Analytical Chemistry - Abstract
DNA methyltransferase (MTase) is an important regulatory enzyme in various biological processes. However, current methods for investigating MTase activity are still limited in terms of sensitivity and/or generality. Herein, we proposed a dual amplification fluorescence strategy for the ultrasensitive detection of DNA adenine methylation methyltransferase (Dam MTase) activity based on strand displacement amplification (SDA) coupled with rolling circle amplification (RCA). In this study, the hairpin probe could not be cleaved by Nt.AlwI nicking endonuclease (Nt.AlwI) in the presence of Dam MTase, and the subsequent SDA-RCA reaction was blocked, resulting in a weak fluorescence signal. Moreover, the blocking effect was more pronounced at a higher concentration of Dam MTase. This assay provides a very low detection limit (down to 0.0067 U ml
- Published
- 2022
3. Benzo[ a ]pyrene promotes progression in tongue squamous cell carcinoma
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Puping Liang, Linxuan Huang, Jianping Yu, Qingjian Chen, Yupeng Yao, Yan Zhang, and Xiaofen Xiao
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Genome instability ,Motility ,medicine.disease_cause ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Tongue ,Gentamicin protection assay ,Cell Movement ,Cell Line, Tumor ,Benzo(a)pyrene ,medicine ,Animals ,General Dentistry ,Carcinogen ,Cisplatin ,Mutation ,030206 dentistry ,Molecular biology ,Tongue Neoplasms ,Otorhinolaryngology ,chemistry ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Wound healing ,medicine.drug - Abstract
Objectives Benzo[a]pyrene (B[a]P) is a member of the polycyclic aromatic hydrocarbon (PAH) family. Although the potent carcinogenicity of high-dose B[a]P has been extensively reported, the effects of long-term exposure to B[a]P on the progression of tongue squamous cell carcinoma (TSCC) are poorly understood. Methods In the present study, TSCC cells were treated with 5 or 50 nM of B[a]P for three months. The proliferation and chemoresistance of B[a]P-treated cells to 5-fluorouracil or cisplatin were detected by CCK8. The motility of the B[a]P-treated cells was evaluated with wound healing analysis, invasion assay, and three-dimensional culture in decellularized mouse tongue matrix. Xenograft assay was used to investigate the aggressiveness of B[a]P-treated cells. Immunofluorescence staining, terminal restriction fragment assay, and whole-genome sequence were used to determine the mutation spectrums. Results Long-term 50 nM B[a]P-treated cells exhibited increased aggressiveness and chemoresistance to 5-fluorouracil or cisplatin. In addition, data from whole-genome sequencing demonstrated that C:T to A:T transitions were the predominant nucleotide substitutions occurred in 50 nM B[a]P-treated CAL27 cells. Furthermore, 102 non-synonymous or stop-gain mutations were enriched in the extracellular-matrix-receptor interactive pathway. Conclusions B[a]P exposure may contribute to genomic instability, and therefore, B[a]P may promote the progression of TSCC.
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- 2020
4. Combined nano/micro-structure of Ni12P5-Ni2P nanorod array for effective wide pH range HER and overall alkaline water-splitting
- Author
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Xiaofen Xiao, Chuangang Hu, Quanbin Dai, Chuanyin Xiong, Dong Liu, and Huile Jin
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General Chemical Engineering ,Electrochemistry ,Analytical Chemistry - Published
- 2022
5. Metagenomic Analysis Reveals
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Xiaofen, Xiao, Shandan, He, Fei, He, Xiaoyun, Wu, and Yuyan, Zheng
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Research Article - Abstract
The progression of early childhood caries (ECC) is caused by microbial colonized in dental plaque. However, the association framework both from 16s genus down to high resolution metagenomic strain level and from composition to genome function analysis on caries lacks. 16S rRNA sequence revealed the composition of 3–6 years dental caries (ECC, n = 29), and severe dental caries (SECC, n = 36) children are significantly different from caries-free controls (CF, n = 31). Especially, genus Neisseria is enriched in caries (P < 0.05). Metagenomics sequence of 3 ECCs, 3 SECCs, and 3 CFs reveals Neisseria bacilliformis ATCC BAA-1200 in genus Neisseria is also significantly enriched in caries (P < 0.05). Then, we recovered high-quality metagenomic assembly genomes (MAG), named bin 86, which have 99% identity with Neisseria bacilliformis ATCC BAA-1200 genome. Function analysis of Neisseria bacilliformis ATCC BAA-1200 genome shows its metabolism power of sugar and adhesion, colonization, acid production, and acid tolerance ability, which suggested Neisseria bacilliformis ATCC BAA-1200 may serve as a biomarker for childhood caries.
- Published
- 2021
6. Electrochemical assay for continuous monitoring of dynamic DNA methylation process
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Yuzhi Xu, Xiaoyong Zou, Zong Dai, Yingjun Ma, Li Zhang, Xiaofen Xiao, Jun Chen, and Danping Chen
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Models, Molecular ,Base pair ,Drug Evaluation, Preclinical ,Biomedical Engineering ,Biophysics ,Metal Nanoparticles ,Biosensing Techniques ,Biology ,010402 general chemistry ,01 natural sciences ,Catechin ,DNA Methyltransferase 3A ,Electron Transport ,chemistry.chemical_compound ,Electrochemistry ,Humans ,DNA (Cytosine-5-)-Methyltransferases ,Enzyme Inhibitors ,Detection limit ,Hybridization probe ,010401 analytical chemistry ,Electrochemical Techniques ,General Medicine ,Methylation ,DNA Methylation ,Molecular biology ,0104 chemical sciences ,Electrochemical gas sensor ,chemistry ,DNA methylation ,Gold ,Cytosine ,DNA ,Biotechnology - Abstract
A simple electrochemical strategy is reported for continuous monitoring of dynamic DNA methylation process over time. An electrochemical sensor was prepared by co-assembling of DNA probe and 6-mercapto-1-hexanol onto a gold electrode. The top of the DNA probe was labeled with 6-ferrocenylhexanethiol modified gold nanoparticle. The charge density between the C•G base pair was verified to be slightly reduced by DNA methylation, and could be further decelerated by ~ 25% upon co-locating a Br group onto methylated cytosine (mC). Therefore, in the presence of NaIO4/LiBr, the progressively methylated DNA on the sensor showed a clearly decreasing current over methylation time. The dynamic DNA methylation process was indicated continuously from the current decrease ratio, with a limit of detection of 0.0372µM. The strategy is convenient, cost-effective, and enable continuous profiling methylation process without distortion. Besides, the strategy was successfully applied for the studies on inhibitor screening and flanking sequence preference of DNA methyltransferase 3a. The results show that the activity of DNA methyltransferase 3a can be mildly inhibited by epigallocatechin gallate, and varies towards different flanking sequence with an order of 5'-CCGG-3' < 5'-CGCG-3' < 5'-CGCA-3'.
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- 2018
7. Correction to: Changes before and after COVID-19 pandemic on the personal hygiene behaviors and incidence of peritonitis in peritoneal-dialysis patients: a multi-center retrospective study
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Yanglin Hu, Li Xu, XiaoHui Wang, Xiaofei Jin, Sheng Wan, Qing Luo, Yanqiong Ding, Xiaofen Xiao, and Fei Xiong
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Nephrology ,Urology - Published
- 2021
8. A general approach to cobalt-based homobimetallic phosphide ultrathin nanosheets for highly efficient oxygen evolution in alkaline media
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Qiang Zhang, Zhongke Yuan, Chun-Ting He, Liming Dai, Jing Li, Shuangyin Wang, Xiaofen Xiao, Shenlong Zhao, Wensheng Lin, and Dingshan Yu
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Mass transport ,Materials science ,Renewable Energy, Sustainability and the Environment ,Phosphide ,Inorganic chemistry ,Oxygen evolution ,chemistry.chemical_element ,02 engineering and technology ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Pollution ,0104 chemical sciences ,chemistry.chemical_compound ,Nuclear Energy and Engineering ,chemistry ,Homogeneous ,Environmental Chemistry ,0210 nano-technology ,Porosity ,Bimetallic strip ,Cobalt - Abstract
A general and effective approach was proposed to fabricate a new family of Co-based bimetallic phosphide ultrathin nanosheets (CoM-P-NS, M = Ni, Mn, Cu, Zn) with homogeneous composition and unique porous architecture using ultrathin metal–organic framework nanosheets (MOFNs) as precursors for the first time, which yielded synergistically active sites, mass transport and dynamic modulations for the oxygen evolution reaction (OER). The optimized samples showed remarkable oxygen evolution activity in alkaline electrolytes, outperforming both the commercial RuO2 and Ir/C benchmarks and ranking the best among all the metal-phosphide electrocatalysts reported to date.
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- 2017
9. Graphene-based materials for polymer solar cells
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Zi-Yan Zhang, Wei Hong, Jing Li, Xudong Chen, Xiaofeng Lin, Dingshan Yu, Xiaofen Xiao, and Zhongke Yuan
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Materials science ,Graphene ,Graphene foam ,Energy conversion efficiency ,Nanotechnology ,02 engineering and technology ,General Chemistry ,Conductivity ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Polymer solar cell ,0104 chemical sciences ,law.invention ,law ,Thermal ,Electrode ,0210 nano-technology ,Graphene oxide paper - Abstract
Due to the remarkable electronic, optical, thermal, and mechanical properties, graphene-based materials have shown great potential in a wide range of technique applications. Particularly, the high transparency, conductivity, flexibility, and abundance make graphene materials highly attractive for polymer solar cells (PSCs). Graphene-based materials have been regarded as one promising candidate used in various parts in PSCs not only as electrodes, but also as interfacial layers and active layers with an aim to boost the power conversion efficiency of the devices. In this review, we summarize the recent progress about the design and synthesis of graphene-based materials for efficient PSCs along with the related challenges and future perspectives.
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- 2016
10. Self-Assembled Graphene Nanostructures and Their Applications
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Xiaofen Xiao, Zhongke Yuan, Dingshan Yu, and Quan Li
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Nanostructure ,Materials science ,Nanocomposite ,Graphene ,law ,Nanotechnology ,Electrocatalyst ,Energy storage ,Self assembled ,law.invention - Published
- 2018
11. ChemInform Abstract: Graphene-Based Materials for Polymer Solar Cells
- Author
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Xiaofen Xiao, Wei Hong, Xudong Chen, Jing Li, Xiaofeng Lin, Dingshan Yu, Zi-Yan Zhang, and Zhongke Yuan
- Subjects
Graphene ,law ,Chemistry ,Electrode ,Energy conversion efficiency ,Thermal ,Energy transformation ,Nanotechnology ,General Medicine ,Conductivity ,Polymer solar cell ,law.invention - Abstract
Due to the remarkable electronic, optical, thermal, and mechanical properties, graphene-based materials have shown great potential in a wide range of technique applications. Particularly, the high transparency, conductivity, flexibility, and abundance make graphene materials highly attractive for polymer solar cells (PSCs). Graphene-based materials have been regarded as one promising candidate used in various parts in PSCs not only as electrodes, but also as interfacial layers and active layers with an aim to boost the power conversion efficiency of the devices. In this review, we summarize the recent progress about the design and synthesis of graphene-based materials for efficient PSCs along with the related challenges and future perspectives.
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- 2016
12. Self-Assembled Graphene-Based Architectures and Their Applications
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Zhe Zhao, Jing Li, Zhongke Yuan, Quan Li, Dingshan Yu, and Xiaofen Xiao
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energy conversion ,Nanostructure ,Materials science ,optoelectronics ,General Chemical Engineering ,Reviews ,General Physics and Astronomy ,Medicine (miscellaneous) ,Nanotechnology ,Review ,02 engineering and technology ,Related derivatives ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,law.invention ,Self assembled ,symbols.namesake ,law ,Energy transformation ,General Materials Science ,Electronics ,catalysis ,energy storage ,Graphene ,General Engineering ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrostatic attraction ,self‐assembled graphene ,symbols ,van der Waals force ,0210 nano-technology ,hybrid structures - Abstract
Due to unique planar structures and remarkable thermal, electronic, and mechanical properties, chemically modified graphenes (CMGs) such as graphene oxides, reduced graphene oxides, and the related derivatives are recognized as the attractive building blocks for “bottom‐up” nanotechnology, while self‐assembly of CMGs has emerged as one of the most promising approaches to construct advanced functional materials/systems based on graphene. By virtue of a variety of noncovalent forces like hydrogen bonding, van der Waals interaction, metal‐to‐ligand bonds, electrostatic attraction, hydrophobic–hydrophilic interactions, and π–π interactions, the CMGs bearing various functional groups are highly desirable for the assemblies with themselves and a variety of organic and/or inorganic species which can yield various hierarchical nanostructures and macroscopic composites endowed with unique structures, properties, and functions for widespread technological applications such as electronics, optoelectronics, electrocatalysis/photocatalysis, environment, and energy storage and conversion. In this review, significant recent advances concerning the self‐assembly of CMGs are summarized, and the broad applications of self‐assembled graphene‐based materials as well as some future opportunities and challenges in this vibrant area are elucidated.
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- 2017
13. Chemical-oxidation cleavage triggered isothermal exponential amplification reaction for attomole gene-specific methylation analysis
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Xiaoyong Zou, Yuzhi Xu, Wenyuan Zhu, Zong Dai, Xiaofen Xiao, and Chang Niu
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Molecular Sequence Data ,Cleavage (embryo) ,Fluorescence ,Mass Spectrometry ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,law ,Limit of Detection ,Gene expression ,Electrochemistry ,Humans ,DNA Cleavage ,Organic Chemicals ,Polymerase chain reaction ,Base Sequence ,Methylation ,Nucleic acid amplification technique ,DNA ,DNA Methylation ,Molecular biology ,Kinetics ,chemistry ,DNA methylation ,Nucleic Acid Amplification Techniques ,Oxidation-Reduction ,Cytosine - Abstract
Genomic 5-methylcytosine (5-mC) modification is known to extensively regulate gene expression. The sensitive and convenient analysis of gene-specific methylation is wishful but challenging due to the lack of means that can sensitively and sequence-selectively discriminate 5-mC from cytosine without the need for polymerase chain reaction. Here we report a chemical-oxidation cleavage triggered exponential amplification reaction (EXPAR) method named COEXPAR for gene-specific methylation analysis. EXPAR was proved to not only have rapid amplification kinetics under isothermal condition but also show excellent sequence-selectivity and linear-dependence on EXPAR trigger. Further initiation of EXPAR by chemical-cleavage of DNA at 5-mC, the COEXPAR showed high specificity for methylated and nonmethylated DNA, and ∼10(7) copies of triggers were replicated in 20 min, which were used to quantify the methylation level at the methylation loci. As a result, the gene-specific methylation level of a p53 gene fragment, as a target model, was analyzed in two linear ranges of 10 fM-1 pM and 1 pM-10 nM, and limits of detection of 411 aM (S/N = 3) by fluorescence, and 576 aM (S/N = 3) by electrochemistry. The method fulfilled the assay in an isothermal way in ∼5 h without the need for tedious sample preparation and accurate thermocycling equipment, which is likely to be a facile and ultrasensitive way for gene-specific methylation analysis.
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- 2015
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