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Background: Metabolic acidosis plays a key role in transient global cerebral ischemiareperfusion (I/R) induced delayed neuronal death (DND) of the hippocampal CA1 region of gerbils. Na+ coupled HCO3 - transporters (NBCs) mediated Na+/HCO3 - co-transportation can be activated by the pH gradient of intracellular and extracellular environments induced by acidosis. However, whether NBCs are activated and involved in I/R-induced neuronal injury is unknown. Objective: In this work, we studied neuronal apoptosis, astrocyte activation, and hippocampusdependent memory task using a well-established transient global cerebral I/R model of gerbils and investigated whether the specific NBCs inhibitor S0859 could reverse this injury. Methods: To explore the role of S0859 in I/R-induced DND, we established a transient global cerebral I/R model of Mongolian gerbils and studied neuronal apoptosis by using Nissl stain and TUNEL assay. The excitability and NBCs current were analyzed by whole-cell patch-clamp, while the cognitive function was evaluated by Barnes maze. Results: We found that I/R increased the NBCs current, inhibited the excitability of CA1 neurons, and led to apoptosis in CA1 neurons. Selective NBCs inhibitor S0859 protected CA1 neurons from I/R induced neuronal cell death, astrocyte accumulation, and spatial memory impairment. Conclusion: These findings indicate that NBCs mediate transient global cerebral I/R induced DND of CA1 neurons, and NBCs inhibitors could be a promising target to protect neuronal functions after I/R.

The trip graph, which can model the residents’ taxi demands, consists of vertices used to indicate trips and edges used to indicate the follow-up relationships between trips. A trip $$v_j$$ v j is a tight follow-up trip of another trip $$v_i$$ v i , if a taxi can arrive at the departure location of $$v_j$$ v j within a time threshold $$\delta$$ δ after it finishes $$v_i$$ v i . However, for big cities, there are a large amount of trips every day and it is time consuming to construct a trip graph. In this paper, we propose efficient algorithms to construct trip graphs for big cities. When constructing a trip graph, the most expensive step is to connect the vertices if the tight follow-up relationships exist. To find out the tight follow-up trips fast, we design an index considering both spatial and temporal constraints. To designate appropriate search areas, we propose efficient methods to determine the distance-based search areas and the traffic-based search areas. We conduct experiments on real datasets, i.e., the taxi trajectories of Shanghai in 2015. The experimental results show that our algorithm can construct the trip graph about 40 times faster than the straightforward method. We also demonstrate the usages of the trip graph in green transportation applications, i.e., the minimum fleet analysis and the minimum total “idle” mileage analysis.

The advanced lithium storage performance of the P-doped self-assembled V2C MXene/NiCo-LDH hybrid benefits from high capacitive contribution, high electrical conductivity, and good structural stability.

The rising number of cyberattacks and cybercriminals around the world has put major threats on the cybersecurity and even the national security of many countries. In response, governments started to introduce policies and regulations to improve cybersecurity. It is increasingly realized that effective cybersecurity policy making largely depends upon optimized policy attention and the effective use of public resources. This article draws on the “attention-driven policy choice model” and the punctuated equilibrium theory to analyze the relationship between policy attention allocation, policy agenda setting, and the choice of policy tools in different periods of cybersecurity development. Using Chinese cybersecurity development as the main context, content analysis of policy texts was conducted to examine the pathway of cybersecurity policy development in China between 1994 and 2019. The findings suggest that there is evidence for punctuated equilibrium with policy stagnation and incrementality being broken up by major events which shift policy attention and subsequently affects agenda setting and the choice of policy tools. The findings of this article will provide important implications for the development of strategic foresight of cybersecurity and the effective use of public resources to improve cybersecurity.

Corneal stem/progenitor cells are typical adult stem/progenitor cells. The human cornea covers the front of the eyeball, which protects the eye from the outside environment while allowing vision. The location and function demand the cornea to maintain its transparency and to continuously renew its epithelial surface by replacing injured or aged cells through a rapid turnover process in which corneal stem/progenitor cells play an important role. Corneal stem/progenitor cells include mainly corneal epithelial stem cells, corneal endothelial cell progenitors and corneal stromal stem cells. Since the discovery of corneal epithelial stem cells (also known as limbal stem cells) in 1971, an increasing number of markers for corneal stem/progenitor cells have been proposed, but there is no consensus regarding the definitive markers for them. Therefore, the identification, isolation and cultivation of these cells remain challenging without a unified approach. In this review, we systematically introduce the profile of biological characterizations, such as anatomy, characteristics, isolation, cultivation and molecular markers, and clinical applications of the three categories of corneal stem/progenitor cells.

Thirty-four individual thermohaline sheets are identified at depths of 170–400 m in the Canada Basin of the Arctic Ocean by using the hydrographical data measured with the Ice-Tethered Profilers (ITPs) between August 2005 and October 2009. Each sheet is well determined because the salinity within itself remains very stable and the associated salinity anomaly is markedly smaller than the salinity jump between neighboring sheets. These thermohaline sheets are nested between the Lower Halocline Water (LHW) and Atlantic Water (AW) with lateral coherence of hundreds of kilometers and thickness varying from several to dozens of meters. The physical properties, including temperature, heat flux, and vertical turbulent diffusivity, in the sheet are found to be averagely associated with the AW propagation. Spatially, the thermohaline sheet is in a bowl-shaped distribution, which is deepest in the basin center and gradually becomes shallower toward the periphery. The interaction between the LHW and AW could be demonstrated through the property variances in the sheets. The temperature variances in the upper and lower sheets are correlated with the LHW and AW, respectively, transited at the 15th sheet, whereas the depth variance in the sheet is strongly correlated with the LHW. It is proposed that the sheet spatial distribution is mainly dominated by the Ekman convergence with the Beaufort Gyre, slightly modulated with the AW intrusion. Significance Statement The diffusive convection staircases, composed of consecutive steps containing thick mixed layers and relatively thin interfaces, are prominent between the Lower Halocline Water (LHW) and the Atlantic Water (AW) throughout the Canada Basin. This sheet-like structure is in a bowl shape with lateral coherence over hundreds of kilometers. It is proposed that the distribution of the thermohaline sheet is mainly dominated by the Ekman convergence with Beaufort Gyre, as well as the AW intrusion. The present method of thermohaline-sheet identification would have more implications beyond this work. Since the thermohaline sheet remains mostly stable and coherent on a very large spatial–temporal scale, it might play a similar role as the water mass analysis in numerous applications, e.g., climate change.

Detection of hidden faults and sedimentary layers in the urban subsoil is significant for the utilization of the underground space, earthquake hazard mitigation, and so forth. Guilin, located in southwest China, is well known for the development of the most typical karst landform in the world and has become an international tourist city that needs scientific planning and knowledge of the urban underground space. After collecting waveform data recorded continuously over a period of about 1.5 months by a dense array of 114 short-period seismic stations installed in and around Guilin, we adopt ambient seismic noise cross-correlation method to extract Rayleigh-wave phase velocity dispersion curves within the period range from 0.5 to 5 s and to obtain a high-resolution S-wave velocity (VS) model of the shallow crust above 9 km using surface-wave tomography. The vertical VS gradient image indicates that the sediment thickness of the Guilin Karst basin is about 1–3 km. Sedimentary layers are relatively thick between Yanshan Mountain and Haiyang Mountain, and along the Yi river valley, where karst groundwater may be abundant and used as an important option for urban water supply. Both the absolute VS velocity image and the relative VS anomaly image clearly reveal the occurrence, location, and deep extension characteristics of major faults. Longsheng–Yongfu, Nanning–Guilin, Yaoshan–Yanshan, Baishi, and Guanyang–Hengyang may be regional deep faults cutting through the upper crust at least. The evolution of the basin is mainly controlled by the steep dipping Longsheng–Yongfu and Baishi faults, and partially controlled by the gently dipping Nanning–Guilin fault in the interior of the basin. The Nanning–Guilin fault is an active and partially buried fault obliquely crossing Guilin city. Urban seismic imaging, such as the new VS tomography presented here, can play an important role in understanding tectonic and tectonic-subsidence earthquake hazards associated with these buried faults.

The Notch signaling pathway is related to endothelial dysfunction in coronary atherosclerosis. Our objective was to explore the role of Notch signaling in coronary microvascular dysfunction (CMD). CMD models were constructed by sodium laurate injection in vivo and homocysteine (Hcy) stimulation in vitro. The binding ability of Notch Intracellular Domain (NICD)/H3K9Ac/GCN5 (General Control Non-derepressible 5) to Neuregulin-1 (Nrg-1) promoter was examined by chromatin immunoprecipitation. Immunofluorescence staining was conducted to detect CD31 positive cells, NICD localization, and co-localization of NICD and GCN5. Flow cytometry and Tunel staining were conducted to identify the apoptosis. Hematoxylin and eosin staining, quantitative real-time PCR, western blot, immunohistochemical staining, co-immunoprecipitation, and double luciferase report analysis were also conducted. Notch signaling pathway-related protein levels were decreased, levels of Nrg-1 and the phosphorylation of ErbB2 and ErbB4 were enhanced in CMD models. Interference with Nrg-1 further increased the apoptosis in Hcy-induced cardiac microvascular endothelial cells (CMECs). Meanwhile, the activation of the Notch signaling pathway increased the levels of Nrg-1 and the phosphorylation of ErbB2 and ErbB4, as well as inhibited the apoptosis induced by Hcy. Furthermore, NICD and histone acetyltransferase enzyme GCN5 could regulate Nrg-1 promoter activity by affecting the expression of acetylation-modified protein H3K9Ac. In addition, NICD also interacted with GCN5. In vivo results also confirmed that the activation of the Notch signal alleviated CMD. Notch signaling pathway regulates Nrg-1 level through synergistic interaction with GCN5, thereby mitigating CMD.

Landscape and quality attributes are major ecosystem characteristics closely associated with soil conservation service (SCS). However, the intrinsic mechanisms by which these two attributes influence SCS are still unclear. Therefore, this study quantitatively analyzed the landscape pattern, ecological quality, and SCS in the Lianshui River watershed (a typical soil and water loss area of red soil in southern China) and its sub-watersheds in 2019. The boosted regression tree model was used to explore the influence of 15 factors (i.e., landscape and quality attributes) on SCS at the sub-watershed scale. According to the results, compared with the landscape attribute, the quality attribute of the watershed ecosystem could better explain the spatial heterogeneity of SCS across 66 sub-watersheds. The overall degree of influence of five quality factors on SCS reached 57.81%, with the highest being the normalized differential build-up and bare soil index (NDBSI), at 25.11%. Among 10 landscape factors, aggregation had the greatest influence on SCS, at 28.64%. The relationships between key influencing factors and SCS were non-monotonic and non-linear, with threshold effects. For example, NDBSI values of 0.18–0.41 had a positive influence on SCS, while NDBSI values of 0.41–0.65 had a negative influence on SCS. The findings broaden our understanding of the response of SCS to changes in landscape and quality attributes at the sub-watershed scale, and could offer comprehensive support for soil erosion management in the watershed ecosystem.

Eyewall replacement cycles (ERCs) in tropical cyclones (TCs) are generally associated with rapid changes in TC wind intensity and broadening of the TC wind-field, both of which can create unique forecasting challenges. As part of the NOAA Joint Hurricane Testbed Project, a new model was developed to provide operational probabilistic guidance on ERC onset. The model is based on the time evolution of TC wind-intensity and passive satellite microwave imagery, and is named “M-PERC” for Microwave-based Probability of Eyewall Replacement Cycle. The model was initially developed in the Atlantic basin, but is found to be globally applicable and skillful. The development of M-PERC and its performance characteristics are described here, as well as a new intensity prediction model that extends previous work. Application of these models is expected to contribute to a reduction of TC intensity forecast error.

Background and purposeLower-grade gliomas (LGG) exhibit a wide range of metabolic pathway changes, and metabolic reprogramming can be largely seen as a result of oncogenic driving events. Glycolysis, an important pathway of tumor energy source, has been poorly studied in gliomas. The aim of this article is to analyze the relationship between glycolysis and lower-grade glioma development and prognosis in order to explore the heterogeneous relevance of glycolysis in lower-grade gliomas.Methods and resultsOur study searched the TCGA database and identified three glycolytic subtypes with significant prognostic differences by unsupervised clustering analysis of core glycolytic genes, named C1, C2, and C3. By analysis of clinical prognosis, somatic cell variation, and immune infiltration, we found that C3 had the best prognosis with molecular features of IDHmut-codel, followed by C1 with major molecular features of IDHmut-non-codel, G -CIMP high subtype, while C2 had the worst prognosis, mainly exhibiting IDHwt, G-CIMP low and mesenchymal-like subtypes with seven important CNV features, including CDKN2A/B deletion, chr7 gain and chr10 deletion, chr19/20 co-gain, EGFR amplification and PDGFRA/B deletion phenotypes were significantly increased, with the highest level of stemness and significant T-cell depletion features. Finally, to quantify the level of abnormal glycolysis and its impact on prognosis, we developed GlySig to reflect the glycolytic activity of LGG and integrated molecular features to construct nomogram that can be independently assessed to predict prognosis.ConclusionsOur study analyzed the tumor characteristics of different glycolytic states, and our findings explain and describe the heterogeneity of glycolytic metabolism within diffuse LGGs.

Non-infectious chronic diseases, especially inflammatory bowel diseases (IBDs), hypertension, and diabetes mellitus, are characterized by a prolonged and multisystemic course, and their incidence increases annually, usually causing serious economic burden and psychological stress for patients. Therefore, these diseases deserve scientific and consistent disease management. In addition, the lack of a comprehensive “early disease clues tracking—personalized treatment system—follow-up” model in hospitals also exacerbates this dilemma. Based on these facts, we propose an individualized prediction management system for IBDs based on chronic diseases, focusing on the established IBDs-related prediction models and summarizing their advantages and disadvantages. We call on researchers to pay attention to the integration of models with clinical practice and the continuous correction of models to achieve truly individualized medical treatment for chronic diseases, thus providing substantial value for the rapid diagnosis and adequate treatment of chronic diseases such as IBDs, which follow the “relapse-remission” disease model, and realizing long-term drug use and precise disease management for patients. The goal is to achieve a new level of chronic disease management by scientifically improving long-term medication, precise disease management, and individualized medical treatment, effectively prolonging the remission period and reducing morbidity and disability rates.

Delimiting basic farmland is essential to ensure food security and maintain social stability. Determining the quantity and spatial layout of the basic farmland is the main aspect of delimitation. For a long time, superiors in local governments have mainly assigned the basic farmland quantity, which is prone to regional imbalance. The determination of the spatial layout is mainly based on the quality and contiguity of the cultivated land; however, spatial stability is not considered. Therefore, in this study, Yingtan City was used as an example to determine the scale of basic farmland based on the food security and multi-source data, construct a Land Evaluation and Site Assessment System model, evaluate the quality of cultivated land, and integrate the degree of agglomeration and compatibility of cultivated land to characterize its spatial stability. The quality and spatial stability of the cultivated land were combined to construct a matrix. The cultivated land patches must be selected according to the "scale limitation, stability priority, and quality superiority" principle. Finally, basic farmland covering an area of 75,946.34 hm

Objective Arginine ADP-ribosyltransferase 1 (ART1) is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. However, how ART1 regulates glycolysis in CRC remains elusive. Methods To elucidate the role of ART1 in glycolysis in CRC, we assessed the protein level of ART1, hypoxia-inducible factor 1α (HIF1α), and glucose transporter type 1 (GLUT1) in 61 CRC tumor tissue specimens obtained from patients with different 2-[18F]fluoro-2-deoxy-D-glucose (18F-FDG) uptake as analyzed by PET/CT before surgery. Colon adenocarcinoma CT26 cells with ART1 knockdown and overexpression were established, respectively, and the molecular mechanism underlying the effect of ART1 on glycolysis in CRC was determined both in vivo and in vitro. Results The expression of ART1 and GLUT1 was significantly associated with FDG uptake (P=0.037 and P=0.022, respectively) in CRC tissues. Furthermore, the expression of hexokinase 2 (HK2) and lactate dehydrogenase (LDH) was upregulated in ART1-overexpressed CT26 cells, but was downregulated in ART1-knockdown CT26 cells. The volume and weight of subcutaneously transplanted tumors were markedly increased in the ART1-overexpressed BALB/c mice group and decreased in the ART1-knockdown group. In CT26 cells, the overexpression of ART1 promoted the expression levels of HK2 and LDH, and knockdown of ART1 suppressed them in the CT26 tumors. In both normal and hypoxic conditions, ART1 expression was associated with the protein level of phospho-serine/threonine kinase (p-AKT), HIF1α, and GLUT1 but not with that of AKT in CT26 cells and subcutaneous transplanted tumors. Conclusion ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway.

Economic and social development have worsened the situation of water pollution and even the ecological environment. It is helpful to quantify the water pollution ecological environment losses for decision-makers to formulate reasonable pollution control plans. However, the current quantitative analyses led by economic methods are not comprehensive and systematic. Therefore, based on the emergy theory and method system of eco-economics, this study analyzed the internal energy flow process of the water-polluted ecosystem, discussed the composition of water-polluted ecological environment loss, and proposed a quantitative model of water-polluted ecological environment loss based on the emergy analysis method. It can reasonably quantify the ecological environment loss caused by water pollution and provide a reference for optimizing regional industrial layout, scientifically formulating pollution control planning, and promoting the sustainable development of the ecosystem. Taking Kaifeng City in Henan Province as an example, the rationality of the model is verified. The results show that the annual average total energy value of water pollution ecological environment loss in Kaifeng City is 3.83 × 1020sej, equivalent to 145 million yuan (0.76) of Kaifeng's gross domestic product (GDP) in 2018.

The thrombodynamic ratio (TDR) as a composite thromboelastography (TEG) parameter, has been proven to be valuable in multiple diseases. However, the association between TDR and mortality in sepsis has not been studied.One hundred forty-one patients were enrolled in this retrospectively study. TEG was performed immediately at admission. Two cox proportional hazards models were developed for the prediction of 28-day mortality. The C statistic, continuous net reclassification index (cNRI) and integrated discriminatory index (IDI) were calculated to compare the discrimination performance of clinical models with and without the TDR value. The integrated calibration index (ICI) and E50 were calculated to compare the calibration.Patients with lower TDR were more likely to have organ impairments and increased 28-day mortality. The TDR value improved discrimination performance in both Model 1 (C statistic, 0.745 vs 0.735; cNRI 19.4%, p = 0.044; IDI 5.6%, p = 0.012) and Model 2 (C statistic, 0.761 vs 0.751; IDI, 5.1%, p = 0.012). Compared to the calibration curve of Model 1 without TDR, addition of TDR displayed better calibration (ICI, 0.023; E50, 0.021).TDR value significantly predicts 28-day mortality in patients with sepsis and could improve the discrimination and calibration performance of clinical prediction models.

Seven photoaffinity-based and sixteen biotin-based berberine (BBR) probes were constructed and screened for their effects on c-Jun N-terminal protein kinases (JNK) phosphorylation (p-JNK) suppressi...

Sanghuangporus Sanghuang is a fungus species. As a traditional Chinese medicine, it is known for antitumor, antioxidant and anti-inflammatory properties. However, the antiaging effect of S. Sanghuang has not been deeply studied. In this study, the effects of S. Sanghuang extract (SSE) supernatants on the changes of nematode indicators were investigated. The results showed that different concentrations of SSE prolonged the lifespans of nematodes and substantially increased these by 26.41%. In addition, accumulations of lipofuscin were also visibly reduced. The treatment using SSE also played a role in increasing stress resistance, decreasing ROS accumulations and obesity, and enhancing the physique. RT–PCR analysis showed that the SSE treatment upregulated the transcription of daf-16, sir-2.1, daf-2, sod-3 and hsp-16.2, increased the expression of these genes in the insulin/IGF-1 signalling pathway and prolonged the lifespans of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress and provides a theoretical basis for the application of S. Sanghuang in anti-ageing treatments.

BackgroundThe time spent with parents is a crucial factor in the growth of children, and children's well-being is an important indicator of their mental health.MethodsTo promote the children's well-being, this study, which is relying on the data from the 2017 China Time Use Survey (CTUS), explores the relationship between parental time and children's well-being and specific influencing factors.ResultsThe more time parents spent with children, the higher their children's well-being will be (coefficient 0.1020, p < 0.01). The life and leisure time parents spent with children promoted children's well-being (coefficient 0.1020, p < 0.01). The life and leisure time the mother spent with children (coefficient 0.1030, p < 0.05) the life and leisure time (coefficient 0.1790, p < 0.05) and the educational interactions time the father spent with children (coefficient 0.3630, p < 0.10) positively affected children's well-being. The influence of the time parents spent with children on children's well-being was heterogeneous based on their children's academic performance.ConclusionsParental accompaniment is an important determinant of children's well-being. Family education, guidance services, and mental health services should be strengthened, and it is necessary to improve the time spent with children and to pay attention to individual differences in children.

Representativeness of Study Participants

Purpose:The treatment outcome for locally advanced or metastatic soft-tissue sarcoma (STS) remains unsatisfactory. Anlotinib had demonstrated impressive activity in the subsequent-line treatment of STS. This study investigated the combination of anlotinib and epirubicin followed by anlotinib maintenance as first-line treatment for patients with advanced STS.Patients and Methods:This prospective, open-label, single-arm, phase II trial was conducted in Zhongshan Hospital, Fudan University. Eligible patients were ages 18 years or older and had previously untreated, pathologically confirmed, unresectable locally advanced or metastatic STS. All patients received up to six cycles of anlotinib plus epirubicin followed by anlotinib maintenance until disease progression, unacceptable toxicity, or death. The primary endpoint was the progression-free survival (PFS) rate at 6 months. The study was registered on chictr.org (identifier ChiCTR1900024928).Results:From June 2019 to August 2020, 30 patients were enrolled. By December 2021, the median PFS was 11.5 months [95% confidence interval (CI): 8.6–14.4 months], while the median overall survival was not reached (95% CI: NE–NE). The objective response rate was 13.33% and the disease control rate was 80.0%. The most common adverse events (AE) included anemia (43.3%), nausea/vomiting (40.0%), fatigue (36.7%), leukopenia (30.0%), and proteinuria (10.0%), which were mainly of grade 1 or 2. The most frequent grade 3 or 4 AEs were anemia (10.0%), febrile neutropenia (33.3%), hypothyroidism (3.3%), and leukopenia (3.3%). No treatment-related death occurred.Conclusions:The combination of anlotinib and epirubicin followed by anlotinib maintenance demonstrated promising efficacy with a favorable safety profile.

Background Hibiseu manihot of various tissues has a high application value, accumulating more than 65% of unsaturated fatty acids in the seeds as well as high levels of oleic acid. Performing transcriptome analysis is beneficial to reveal the molecular mechanism of fatty acid biosynthesis during the development of Hibiseu manihot seeds. Results Transcriptome analysis was performed using seeds of Hibiseu manihot at 5, 15 and 30 days after flowering. There were 3338 (1320down, 2018up), 6657 (3408down, 3249up), 3851 (2001down, 1850up) differentially expressed genes in 5 DAF vs 15 DAF, 5 DAF vs 30 DAF, 15 DAF vs 30 DAF. GO functional analysis showed that differentially expressed genes were mainly involved in three functional categories bioengineering, molecular function and cellular component. KEGG enrichment analysis showed that the differentially expressed genes were mainly involved in environmental information processes, genetic information processing, metabolism and other metabolic pathways. Nine genes related to fatty acid biosynthesis and oleic acid metabolic pathways were validated by qRT-PCR, and the results showed that the qRT-PCR were consistent with the transcriptome sequencing results. The analysis of fatty acid content in Hibiseu manihot seeds at different stages showed that oleic acid increased and then decreased with seed development, at the same time, linoleic acid decreased first and then increased. and stearic acid content was more stable. It was hypothesized that KASII, SAD, FATA, PDAT and FAD2 might be closely related to the high content of oleic acid in Hibiseu manihot seeds, which laid a molecular foundation for further study on the functions of KASII, SAD, FATA, PDAT and FAD2. Conclusions This study has important significance and reference value for regulating the lipid metabolism pathway of Hibiseu manihot and mining the key genes involved in the lipid synthesis and accumulation of Hibiseu manihot.

It is an important content of environment management to accurately identify the time change and spatial distribution of net anthropogenic nitrogen inputs (NANI) in the river basin. In order to develop a unified management and diverse control strategy that fits the characteristics of the basin, this study establishes the NANI-S model combining the NANI model with the spatial autocorrelation analysis method, which is a quantification-analysis-control process, and takes the 70 prefecture-cities in the Yellow River Basin (YRB) as the study area. The result shows that (1) the NANI of YRB increased first and then decreased with an average NANI value of 6787.59 kg/(km

VEGFA, PlGF and IGF-1 are three main angiogenic factors which play significant roles in embryo implantation. However, the relationship between serum expressions of VEGFA, PlGF and IGF-1 and pregnancy outcomes has not been fully illustrated. In this study, serum specimens were collected precisely on day 7 after the LH surge in a natural non-conception cycle from 38 infertile patients who underwent frozen embryo transfer (FET) treatment. ELISA was used to determine the concentrations of VEGFA, PlGF and IGF-1. Serum levels of VEGFA, PlGF and IGF-1 were compared between patients who conceived (

Lytic polysaccharide monooxygenases (LPMOs) are important enzymes that boost the hydrolysis of recalcitrant polysaccharides, such as chitin. They are found extensively in different insect species and are classified as auxiliary activities family 15 (AA15) LPMOs (LPMO15). Some of them were identified from the insect midgut and proven to act on chitin. However, knowledge about their physiological roles during insect growth and development remains limited. Here, we found that midgut-specific LPMO15s are widely distributed in different insect orders, such as the orthopteran Locusta migratoria and the lepidopteran Bombyx mori. Using L. migratoria as a model insect, the function of midgut-specific LmLPMO15-3 during development was investigated. Double-stranded RNA-mediated downregulation of LmLPMO15-3 expression at the 4th or 5th instar nymph stage severely decreased the survival rate and resulted in lethal phenotypes. Hematoxylin and eosin staining results indicated that the deficient individuals exhibited incompletely digested peritrophic matrix (PM), which suggested that LmLPMO15-3 is essential for the deconstruction of the PM during molting. This study provides direct evidence of the physiological importance of a midgut-specific LPMO15 during insect development. As L. migratoria is one of the most destructive agricultural pests, LmLPMO15-3 is a potential target for pest management.

Background. Visual symptoms are common in patients with migraine, even in interictal periods. The purpose was to assess the association between dynamic functional connectivity (dFC) of the visual cortex and clinical characteristics in migraine without aura (MwoA) patients. Methods. We enrolled fifty-five MwoA patients as well as fifty gender- and age-matched healthy controls. Regional visual cortex alterations were investigated using regional homogeneity (ReHo) and amplitude of low-frequency fluctuation (ALFF). Then, significant regions were selected as seeds for conducting dFC between the visual cortex and the whole brain. Results. Relative to healthy controls, MwoA patients exhibited decreased ReHo and ALFF values in the right lingual gyrus (LG) and increased ALFF values in the prefrontal cortex. The right LG showed abnormal dFC within the visual cortex and with other core brain networks. Additionally, ReHo values for the right LG were correlated with duration of disease and ALFF values of the right inferior frontal gyrus and middle frontal gyrus were correlated with headache frequency and anxiety scores, respectively. Moreover, the abnormal dFC of the right LG with bilateral cuneus was positively correlated with anxiety scores. Conclusions. The dFC abnormalities of the visual cortex may be involved in pain integration with multinetworks and associated with anxiety disorder in episodic MwoA patients.

Amorphous RuBx and crystalline Ru nanoparticles integrated into B/N-doped porous carbon polyhedra via a facile one-step thermal-reduction exhibited remarkable HER electrocatalytic performances in both alkaline and acidic media.

In the theoretical part of this work, a calibration strategy for both phase and amplitude calibration of rotating equispaced circular array based on redundant space calibration was introduced. It avoids the challenging implementation of conventional noise-injection networks, especially for a large-scale array, by using the strong self-consistency accomplished by array rotation. This study presents comprehensive simulation results of the proposed calibration strategy, where calibration residuals and image reconstruction errors are evaluated. The phase calibration shows outstanding performance, while the amplitude calibration is found to be biased due to the logarithms used to linearize the system of calibration equations, and the resulting image reconstruction bias is scene-dependent. A bias correction method is proposed accordingly, which requires an a priori regional brightness temperature within the observation scene. The performance of both phase and amplitude calibration take advantage of increasing the number of elements, which promises the system scalability for high spatial resolution applications., The authors would like to thank the anonymous editor and reviewers for their constructive comments and suggestions. This collaboration was also supported by the Joint Ph.D. Training Program from the University of Chinese Academy of Sciences.