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In ovarian carcinogenesis and progression, long non-coding RNAs (lncRNAs) have been shown to have a role, although the underlying processes remain a mystery. By modulating the degree of autophagy in ovarian cancer cells, we sought to learn more about the function lncRNA HOXA11-AS plays in the development of ovarian cancer. The expression of HOXA11-AS in ovarian normal cells and ovarian cancer cell lines was measured using R package and qRT-PCR. Ovarian cancer cells expressed HOXA11-AS substantially higher than normal cells, while cisplatin-resistant cells expressed HOXA11-AS significantly higher than ovarian cancer cells. Next, we studied the prognostic data of HOXA11-AS in ovarian cancer in the Tissue Cancer Genome Atlas (TCGA). In the next step, lentiviral transfection of ovarian cancer cells A2780, OVCAR3, and A2780/DDP (cisplatin-resistant) were performed, and HOXA11-AS knockdown was found to significantly inhibit cell viability, migration, and invasion of A2780 and OVCAR3 cells, and promote apoptosis by CCK-8 assay, transwell assay, cell cycle, and apoptosis assay, and promoted the sensitivity of A2780/DDP cells to cisplatin. It has been shown by the western blot test that HOXA11-AS knockdown increases the amount of cellular autophagy in cells. In contrast, adding the autophagy inhibitor 3-methyladenine (3-MA) to HOXA11-AS cells knocked down in vivo reduced its anti-tumor properties. As a whole, this study found that HOXA11-AS knockdown increased the expression of autophagy-related proteins and improved cisplatin sensitivity, decreased ovarian cancer cell proliferation, and promoted cell apoptosis. This study provides new insights into the role of HOXA11-AS in ovarian cancer regulation.

In this paper, the pretreatment, concentration reduction and evaporation crystallization stages of desulfurization wastewater in zero discharge technology were studied and engineering applications were carried out, and the performance of 660MW coal-fired unit zero discharge desulfurization wastewater was evaluated. The results showed that for desulphurization wastewater with a total dissolved solid of about 48768.9 mg/L and a Cl content of about 12507.2mg/L, the pass flue gas evaporation process could realize zero discharge treatment of desulphurization wastewater. The hot smoke content introduced into the drying tower was about 52351.0m3/h, the inlet and outlet smoke temperatures of the drying tower were 374°C and 130°C respectively, the influence on boiler efficiency was about 0.0676%, and the moisture content of drying products was only 0.15%. The content of HCl in the flue gas at the inlet and outlet of the drying tower was 53mg/L and 165mg/L, the mass fraction of Cl removal and volatilization of desulfurized wastewater were 93.10% and 6.90%, the average content of chlorine in fly ash was about 0.225%, which meet the requirements of relevant standards for the comprehensive utilization of fly ash.