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Tongboshan Nature Reserve (between 28°03′30′′ - 28°10′33′′N and 118°12′00′′ - 118°21′36′′E) is located in the northeast of the Wuyi Mountain Range in the eastern Jiangxi Province. The fish fauna of mountain streams in the nature reserve was investigated seasonally during 2012. A total of 442 samples were collected and classified into four orders, eight families and 22 species. None of them collected in the nature reserve was exotic species. Among them, Zacco platypus was the most abundant fish species collected, followed by Onychostoma barbatulu and Acrossocheilus parallens. A total of 10 species were found to be endemic to China. Current threats to conservation of fishes in the nature reserve were identified and management solutions are suggested. Key words: Tongboshan Nature Reserve, Mountain streams, ichthyofauna, diversity, conservation.

To optimize somatic cell nuclear transfer (SCNT) procedures in mini-pigs, the present study was designed to examine the effects of donor cell types and aphidicolin (APC) treatment on in vitro development of reconstructed embryos. Oviduct epithelial cells (OEC), ear fibroblast cells (EFC) and cumulus cells (CC) derived from mini-pigs were treated with serum starvation only or serum starvation followed by treatment of 0.1 µg/mL APC. The reconstructed embryos were cultured for 7 days to evaluate their developmental competency. Cleavage and blastocyst formation rates of reconstructed embryos derived from the OEC by APC treatment were significantly higher than the serum starvation (61.82% vs. 56.25%, 24.55% vs. 17.86%; P < 0.05). The cleavage rate from the EFC was significantly increased by APC treatment compared to serum starvation only (63.36% vs. 57.01%; P < 0.05). In the ooctyes with the CC, the reconstructed embryos could yield high blastocyst formation rate by APC treatment (29.63%; P < 0.05). In the presence of APC, CC gave rise to the highest cleavage and blastocyst formation rates among the three cell types. Therefore, our results suggest that treatment of CC with serum starvation plus APC prior to nuclear transfer is more suitable in SCNT of mini-pigs.

In March and April 2009, an entirely novel influenza A (H1N1) virus (NIAV) emerged in Mexico and the USA. During the subsequent months, the virus rapidly spread all over the world by person-to-person transmission. In this report, RNA interference (RNAi) was used as an antiviral agent to inhibit NIAV replication in A549 cells. Ten small interfering RNAs (siRNAs) targeting extremely conserved regions among multiple NIAV genomes could effectively block the replication of NIAV strain A/Beijing/01/2009 (H1N1) in A549 cells. This study may be useful to confront the sudden emergence of NIAV infection.

In this paper taking the case of the Computer Specialty in Newly-promoted undergraduate universities, discussed how to create a practice teaching environment for cultivating applied talents. Specifically suggested how to bulid an effectively practice teaching environment in the classroom; how to take employment as the direction to guide the training orientation of students’s practical ability and how to make good use of teaching resources to create a practice platform and so on. Those thoughts and methods proposed in this paper have some references for other specialties’s practice teaching.

In the real world applications, those basic decision rules inducted using rough set theory can’t match a new object, so it can’t always make an accurate decision for the object. In order to improve the matching ability of decision rules to a new object, an algorithm which generates approximate decision rules more efficiently is proposed in this article and applied to the field of filtering spam based on rough set theory. The results in experimental test showed that those approximate decision rules obtained by the algorithm can enhance the matching ability to a new mail. So the algorithm proposed in this paper is believed to be more effective and feasible.

Diamond-like films have been deposited by dc plasma chemical vapor deposition (CVD). During deposition, the characteristics of the plasma have been measured by means of the Langmuir single probe. The structure of the deposits has been studied by x-ray diffraction, Raman spectroscopy and Fourier transform infrared spectroscopy.

In order to study the mechanism of acoustic landmine detection, the propagation characteristics of acoustically coupled seismic waves in shallow soil are investigated. Based on the porosity and compressibility of shallow soil, the influences of parameters including soil porosity and water saturation on the propagation properties of seismic wave are discussed with the model of seismic waves in unsaturated three-phase porous medium. The calculation results show that under the given parameters, the propagation velocity and attenuation coefficient of seismic wave increase with the increase of acoustic frequency. The propagation velocities of longitudinal waves and those of transverse waves respectively decrease and increase with the increase of soil porosity. In addition, the propagation characteristics of acoustically coupled seismic waves become complicated with the increase of water saturation. The feasibility of the analysis method is discussed by comparing the calculation results with the experimental data published previously. As a result, a theoretical foundation is provided for the acoustic-to-seismic coupling mechanism and its application in the study of acoustic resonance landmine detection.

We observe the phenomenon of priority oscillation of the unexpected σ-polarization in high-power Nd:YVO4 ring laser. The severe thermal lens of the σ-polarized lasing, compared with the π-polarized lasing, is the only reason for the phenomenon. By designing a wedge Nd:YVO4 crystal as the gain medium, the unexpected σ-polarization is completely suppressed in the entire range of pump powers, and the polarization stability of the expected π-polarized output is enhanced. With the output power increasing from threshold to the maximum power, no σ-polarization lasing is observed. As a result, 25.3 W of stable single-frequency laser output at 532 nm is experimentally demonstrated.

Dimensionless dispersion curve equation of one-dimensional periodic structure with three oscillators is deduced. It is proved by singularity theory that the topology of the dispersion curves does not change with mass and stiffness parameter. Then the equations of initial and final frequencies of the bands are presented. The method of band gap design is gained consequently. Two examples i.e., the system with the same stiffness and the system with the same mass, are given to verify it. The method may provide the reference for designing the band gap of the photonic and phononic crystals.

Systems with double-loop hysteresis are used increasingly in engineering, but few studies on their dynamics are reported. In this study, the bifurcation characteristics of the primary resonance of a system with double-loop bilinear hysteresis are investigated on the background of a shape memory alloy damper. First, the frequency-amplitude response equation is obtained by using the averaging methods. Then, the influences of the temperature and the amplitude of excitation on amplitude-frequency responses are analyzed by the constrained bifurcation singularity analysis method of non-smooth systems. The calculation results show that the parameter space composed of the temperature and the amplitude of excitation can be divided into 11 regions, which suggest that there are 11 qualitatively different kinds of amplitude-frequency responses to the variation of two parameters. In order to describe and compare the frequency-amplitude response curves conveniently, an encoding rule is proposed to describe their jump phenomena as the frequency sweeps. The above results can guide directly the design of frequency response mode of the system.

Films of amorphous silicon (a-Si:H,Cl) containing about 10 atomic % hydrogen and 2–8 at % chlorine have been prepared using the r.f. glow-discharge technique with a mixture of H 2 and SiCl 4 . The infrared spectra showed in addition to the hydrogen induced absorption bands at 2100, 2000, 840, 890 and 860 cm −1 the following new absorption lines: 545 cm −1 due to SiCl stretching, 787 and 737 cm −1 which might be caused by the Si-HCl bending vibrations. The absolute chlorine content was measured using sulphocyanic mercury spectrophotometric and chlorine ion selective electrode methods.

Small-angle X-ray diffraction study of amorphous a-Si:H/(a-SiNx:H) periodical multilayer thin films and some single layer films has been undertaken. A number of satellite peaks were found in the lower side of Bragg diffraction peaks of multilayer thin films with a less number of periods. A number of diffraction peaks were also found for the small-angle diffraction of single layer films. We have presented a simple formula for calculating the X-ray diffraction intensity of multilayer and single layer films. A satisfactory explaination of experimental results was obtained. Consequently, a simple method for measuring the total thickness of both multilayer and single layer thin films has been presented.

The thermal expansion coefficients (along and normal to the chain direction) of PTS have been measured by differential parallel-plate capacitances from 77 K to 273 K. A sudden change near the phase transition temperature 195 K and a negative expansion coefficient near 200 K along the chain direction have been observed. The causes of these result are discussed qualitati-vly.