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Supplemental material, sj-pdf-1-jcb-10.1177_0271678X211003497 for Telmisartan prevents high-fat diet-induced neurovascular impairments and reduces anxiety-like behavior by Gianna Huber, Mikolaj Ogrodnik, Jan Wenzel, Ines Stölting, Lukas Huber, Olga Will, Eva Peschke, Urte Matschl, Jan-Bernd Hövener, Markus Schwaninger, Diana Jurk and Walter Raasch in Journal of Cerebral Blood Flow & Metabolism

The setup, operational procedures and performance of a cryogen-free device for producing hyperpolarized contrast agents using dissolution dynamic nuclear polarization (dDNP) in a preclinical imaging center is described. The polarization was optimized using the solid-state, DNP-enhanced NMR signal to calibrate the sample position, microwave and NMR frequency and power and flip angle. The polarization of a standard formulation to yield similar to 4 mL, 60 mM 1-C-13-pyruvic acid in an aqueous solution was quantified in five experiments to P(C-13) = (38 +/- 6) % (19 +/- 1) s after dissolution. The mono-exponential time constant of the build-up of the solid-state polarization was quantified to (1032 +/- 22) s. We achieved a duty cycle of 1.5 h that includes sample loading, monitoring the polarization build-up, dissolution and preparation for the next run. After injection of the contrast agent in vivo, pyruvate, pyruvate hydrate, lactate, and alanine were observed, by measuring metabolite maps. Based on this work sequence, hyperpolarized N-15 urea was obtained (P(N-15) = (5.6 +/- 0.8) % (30 +/- 3) s after dissolution).