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Additional file 1. Supplementary Fig. 1. Representative images of cells with dysmorphic nuclie in monolayers. (a) Immunofluorescence microscopy (IFM) showed deposition of complement on cells with normal nuclei, while a cell with a dysmorphic nucleus in the same field showed decreased complement deposition. (b) Differential interference contrast (DIC) image shows a shrunken cell with a different pattern of complement staining as shown with IFM than cells with normal morphology. (c) Superimposed DIC and IFM images shows that cells with abnormal morphology and nuclei do not deposit complement as cells with normal morphology. Supplementary Fig. 2. EGFR expression and sensitivity to Iressa. (a) qPCR measured normalized EGFR mRNA in 4 HNSCC cell lines, each triangle represents a monolayer. (b) Growth inhibition following 5 μM and 10 μM Iressa treatment was measured at 24 h and 48 h, and the average is represented for each cell lines in the bar graph. Uninhibited control growth is set to 100%. Supplementary Fig. 3. Radioactive C1q binding assay was performed on HN4 and HN5 cell lines, after 48 h of EGFR inhibition using 10 μmol/L Iressa. No significant difference in binding between control and Iressa treated cells was found

Karyogram from LU-HNSCC-26. (PDF 73 kb)

Partial sequencing results for TP53. The LU-HNSSC-26 is homozygous for the single nucleotide polymorphism chr17:7579472Gâ >â C (rs1042522). Partial sequencing results for TP53 mapped against GRCh37 (top) and Sanger sequencing electropherogram (bottom) with the rs1042522 indicated by arrows. (PDF 172 kb)