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Atherosclerosis (AS) is the typical cardiovascular disease, which is the main underlying inducement of cardiovascular diseases. Aberrant expression of long noncoding RNA HLA complex group 11 (HCG11) was engaged with atherosclerosis. The objective of the present research was to explore the role and the potential mechanism of HCG11 in AS. Human umbilical vein endothelial cells (HUVECs) were stimulated with oxidized low-density lipoprotein (ox-LDL) to induce the AS model in vitro. The cell viability was detected by MTT assay. Flow cytometry was performed to determine cell pyroptosis. Gene and protein levels were detected by qPCR or Western blot assay. The interaction between HCG11, miR-224-3p, and Janus kinase 1 (JAK1) was validated by dual-luciferase reporter assays. Ox-LDL treatment aggravated cell pyroptosis and inflammation in HUVECs. And the levels of HCG11 and JAK1 was enhanced in ox-LDL-induced HUVECs, while miR-224-3p expression was reduced. Additionally, knockdown of HCG11 or miR-224-3p overexpression reversed the ox-LDL-induced cell viability decline and the increase of cell pyroptosis and inflammation-related proteins, including gasdermin D N-terminal (GSDMD-N), Caspase-1, NOD-like receptor family pyrin domain-containing 3 (NLRP3), interleukin 18 (IL-18), and interleukin 1beta (IL-1β). Moreover, HCG11 could modulate the JAK1 expression via targeting miR-224-3p. The inhibitory effect of HCG11 silencing on cell pyroptosis and inflammation was reversed by miR-224-3p knockdown. Furthermore, overexpression of miR-224-3p could repress the ox-LDL-induced cell pyroptosis and inflammation via regulating JAK1 expression. Knockdown of HCG11 alleviated cell pyroptosis and inflammation induced by ox-LDL via targeting the miR-224-3p/JAK1 axis, indicating that HCG11 could be the latent target of diagnosis or treatment for AS.

Remote C-H functionalization at C5 is the most sparingly observed selectivity in the functionalization of indole templates. Herein, we reported that the combination of a AgSbF6 catalyst and phenyliodine diacetate oxidation enabled the C-H selenylation at the C5 position of indole scaffolds in a selective version, thus leading to the formation of a wide scope of 5-selenylated indole derivatives, which are otherwise difficult to prepare. Mechanistic studies indicated that current transformation follows a radical process, and the tethered C3 pivaloyl group on indole scaffolds plays roles in both blocking the active C3 position and manipulating the electronic affinity of the arenes.

This paper discuss the fire objective of commercial center integrating the shopping mall and Wal-Mart supermarket etc., and also the auxiliary engineering project of commercial and residential buildings composed by high-rise residential areas. "Fuel Island" design method is adopted to prevent the radiation and spread across functional areas including dining area and commercial area. The maximum heat release rate of fires at various scenes is also determined by different approaches, and then the larger value is taken as the final fire scale.

Biological invasion is a pervasive negative force of global change, especially in its effects on sensitive freshwater ecosystems. Even protected areas are usually not immune. Ptychobarbus chungtienensis is a threatened freshwater fish now almost confined to Bita Lake, in the Shangri-La region of Yunnan province, China. Its existence is threatened by the introduction of non-native weatherfishes (Misgurnus anguillicaudatus and Paramisgurnus dabryanus) by an unusual method known as ‘prayer animal release’. Periodic surveys revealed the ratio of invasive weatherfishes to P. chungtienensis has been increasing since the former species was first recorded from the lake in August, 2009. Ptychobarbus chungtienensis shows low genetic diversity in the relict Lake Bita population. Weatherfishes, however, have highly successful survival strategies. The degree of dietary overlap between the species is alarming and perhaps critical if food is found to be a limiting factor.

In order to realize large-scale absolute surface reconstruction, a generalized iterative optimization method for solving the three-flat problem is studied. First, the idea of model-based absolute surface reconstruction is proposed, which considers the problems of absolute surface reconstruction as inverse problems. Then we take the three-flat problems as an example, we introduced two generalized iterative optimization methods for three-flat model. Finally, by both simulation and experiment, it is concluded that the block SOR method with an optimal relaxation factor converges much faster and saves more computational costs and memory space without reducing accuracy. Both simulation and experimental results indicate that the proposed iterative optimization methods are effective for solving the three-flat problem with pixel-level spatial resolution and the measuring precision of two separate measurements is 0.6 nm rms, and the cross-check test result is 0.8 nm rms. It is concluded that the proposed method can correctly reconstruct absolute figures with high efficiency and pixel-level spatial resolution.

The effects of nitric oxide (NO) and exogenous ethylene on ethylene biosynthesis in harvested Feicheng peaches were studied. The antagonistic actions between NO and exogenous ethylene was also investigated. The Feicheng peaches were fumigated with 10μL L^(-1) NO, 1000μL L^(-1) ethylene, or 10μL L^(-1) NO plus 1000μL L^(-1) ethylene for 3 h. The results suggested that application of exogenous ethylene promoted the biosynthesis of endogenous ethylene in peach fruit. The treatment with NO remarkably inhibited the biosynthesis of ethylene by significantly reducing the activities of ACC synthase (ACS) and ACC oxidase (ACO). Ethylene biosynthesis in the fruits treated with both NO and exogenous ethylene was lower than that in fruits treated with exogenous ethylene alone but higher than that in fruits treated with NO alone, suggesting that there were antagonistic actions between NO and exogenous ethylene. NO could inhibit the biosynthesis of ethylene and the catalysis of exogenous ethylene during ethylene biosynthesis in peach fruits.

Novel supported palladium catalysts were prepared through organometallic Pd compounds including dichlorobistriphenylphosphine palladium (DCTPPP) and dithiocyanatobistriphenylphosphine palladium (DTTPPP) for the hydrodechlorination of CFC-12. These catalysts exhibited excellent catalytic activities in hydrodechlorination of CFC-12. Particularly, high selectivities of CH2F2 formation were achieved over the catalysts, DCTPPP/MgF2 with 88.1% and DTTPPP/MgF2 with 85.8%, at a limited conversion level (

To reveal the effects of three days' repeated exhausted eccentric exercise on the skeletal muscle apoptosis and proliferation in rats.Fifty male SD rats aged at 8 week old were randomly divided into control group (C) and training groups (B1, B2, B3, B4) (n = 10), the training groups ran on a treadmill every day till exhausted. After they had been trained repeatedly for three days, their medial head of triceps brachii muscle cell apoptosis was detected in paraffin section by the TUNEL, expression of proliferating cell nuclear antigen (PCNA) protein was examined by immunohistochemistry.(1) The apoptosis appeared sequential change, and it was consistent with the exercise-induced skeletal muscle micro-injury (EIMmI). The apoptosis index in the training group after exercise was much greater than that in the control group (P0.05), and it reached the peak at 24 h after exercise, then it reduced at 48 h after exercise. (2) The express of PCNA exhibited a sequential change after exercise, the proliferation index in the training group after exercise was greater than that in the control group (P0.05), it increased after exercise immediately, but it reduced at 3 h after exercise, then was reached the peak at 24 h after exercise, the proliferation index was moderately correlated with the apoptosis index (P0.05).(1) Cell apoptosis can induce the delayed skeletal muscle damage. (2) Apoptosis may be a start factor of skeletal muscle regeneration.