1. Effect of Fermentation Time on Antioxidant and Anti-Ageing Properties of Green Coffee Kombucha Ferments
- Author
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Zarębska Magdalena, Hordyjewicz-Baran Zofia, Ziemlewska Aleksandra, Wasilewski Tomasz, Zagórska-Dziok Martyna, Nizioł-Łukaszewska Zofia, and Bujak Tomasz
- Subjects
Kombucha ,Time Factors ,Antioxidant ,030309 nutrition & dietetics ,medicine.medical_treatment ,Intracellular Space ,antioxidant activity ,Pharmaceutical Science ,chemia organiczna ,Coffee ,Antioxidants ,Analytical Chemistry ,Ingredient ,Limit of Detection ,Drug Discovery ,HaCaT Cells ,Food science ,Skin ,0303 health sciences ,Mushroom ,fermentacja ,Pancreatic Elastase ,biology ,Chemistry ,food and beverages ,Kombucha Tea ,04 agricultural and veterinary sciences ,kawa ,040401 food science ,Chemistry (miscellaneous) ,Green coffee extract ,kombucha ,Molecular Medicine ,Cell Survival ,Matrix Metalloproteinase Inhibitors ,inżynieria chemiczna ,komubucha ,Fluorescence ,Article ,lcsh:QD241-441 ,03 medical and health sciences ,0404 agricultural biotechnology ,Phenols ,Picrates ,lcsh:Organic chemistry ,Oxazines ,medicine ,Humans ,Collagenases ,Physical and Theoretical Chemistry ,matrix metallopeptidases ,Flavonoids ,Superoxide Dismutase ,Biphenyl Compounds ,Organic Chemistry ,ferment ,Fibroblasts ,biology.organism_classification ,Water Loss, Insensible ,Yeast ,carbohydrates (lipids) ,Kinetics ,Xanthenes ,green coffee ,Fermentation ,Reactive Oxygen Species ,Bacteria - Abstract
Kombucha, also known as the Manchurian mushroom, is a symbiotic culture of bacteria and yeast, the so-called SCOBY. This paper presents a comprehensive evaluation of the ferments obtained from green coffee beans after different fermentation times with kombucha. Results for the ferments were compared to the green coffee extract that was not fermented. In this study, the antioxidant potential of obtained ferments was analyzed by assessing the scavenging of external and intracellular free radicals and the assessment of superoxide dismutase activity. Cytotoxicity of ferments on keratinocyte and fibroblast cell lines was assessed as well as anti-aging properties by determining their ability to inhibit the activity of collagenase and elastase enzymes. In addition, the composition of the obtained ferments and the extract was determined, as well as their influence on skin hydration and transepidermal water loss (TEWL) after application of samples on the skin. It has been shown that the fermentation time has a positive effect on the content of bioactive compounds and antioxidant properties. The highest values were recorded for the tested samples after 28 days of fermentation. After 14 days of the fermentation process, it was observed that the analyzed ferments were characterized by low cytotoxicity to keratinocytes and fibroblasts. On the other hand, the short fermentation time of 7 days had a negative effect on the properties of the analyzed ferments. The obtained results indicate that both green coffee extracts and ferments can be an innovative ingredient of cosmetic products.
- Published
- 2020
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