1. Additional file 1 of Neutrophil membrane-derived nanoparticle loading TLR7 agonists augments radiotherapy efficacy and boosts abscopal effect via regulating tumor microenvironment
- Author
-
Lu, Dehua, Xu, Yanming, Yang, Xiaodong, Li, Ying, Li, Mengqing, Zheng, Yuanyuan, Wang, Yuenan, Wang, Weihu, Wang, Shubin, Gao, Jing, and Liu, Yajie
- Abstract
Additional file 1. This file includes:Additional Materials and Methods, Figures S1 to S6. Figure S1. Characterizations of DyLight594 labeled R837@PLGA@Neu and DyLight649 labeled R837@PLGA@Neu. (A) Hydrodynamic diameters of DyLight594 labeled R837@PLGA. (B) Hydrodynamic diameters of DyLight594 labeled R837@PLGA@Neu. (C) Hydrodynamic diameters of DyLight649 labeled R837@PLGA. (D) Hydrodynamic diameters of DyLight649 labeled R837@PLGA@Neu. Figure S2. Tumor growth curve of each mouse bearing unilateral CT26 tumor after various treatments. (A)PBS-control; (B)R837@PLGA; (C) R837@PLGA@Neu; (D) RT; (E) R837@PLGA combined with RT; (F) R837@PLGA@Neu combined with RT. Figure S3. H&E staining of organ tissue sections after various treatments. Scale bar: 100 µm. Figure S4. Cell gating of flow cytometric analysis of T cells and DCs in CT26 tumors after treatments. (A) Lymphocytes were gated in accordance with cell size. Then T cells were gated in CD45+ cell population to analyze CD4+ and CD8+ T cell proportion. (B) DCs were analyzed in CD11c+ cell population, and mature DCs were defined as CD80+CD86+ cell population. Figure S5. Flow cytometric analysis of macrophages in CT26 tumors after treatments. (A) Macrophages were determined by F4/80 staining. F4/80+CD206- population was defined as type 1 macrophage (M1), and F4/80+CD206+ population was defined as type 2 macrophage (M2). (B) Proportion of M1 in tumors after various treatments. (C) Proportion of M2 in tumors after various treatments. Numerical data are presented as mean ± SD. *P < 0.05. Figure S6. Representative flow cytometric plots of CD4+ and CD8+ T cells in nonirradiated tumors of bilateral tumors bearing mice treated by RT, R837@PLGA combined with RT, and R837@PLGA@Neu combined with RT.
- Published
- 2023
- Full Text
- View/download PDF