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Based on the reverse austenite transformation process of cold rolled low carbon steel and the deformation energy storage, the mathematical model of phase transformation temperature and structure transformation of austenite reverse transformation was established by using Scheil transformation kinetics. The different heating temperature of austenite and deformation amount of the austenite reverse transformation structure are numerically simulated. The inverse transformation process of austenite was calculated by Matlab, the calculation results show that the austenite transformation temperature AS increases with the increase of heating rate and comes to a constant value. The influence of deformation on AS decreases with the increase of heating rate.Austenite grain size and the temperature is approximately exponential. The accuracy of the model was verified by experimental analysis of the microstructure under different process conditions.

An electrochemical equation suitable for examining the interaction of irreversible redox compounds with DNA is established. According to the equation, diffusion coefficients of both free and binding compounds (Df , Db), binding constant (K) and binding site size (s) of compounds with DNA could be obtained simultaneously by nonlinear fit analysis of electrochemical data. Bis-benzimidazole derivative (Hoechst 33258), as an irreversible redox compound, was investigated for its electrochemical behavior and the interaction with natural fish sperm DNA (fsDNA) using cyclic voltammetry, chronocoulometry, bulk electrolysis and scanning electrochemical microscope technique. A nonlinear fit analysis of the experimental data yielded: Df=8.3×10−5 cm2 s−1, Db=6.0×10−6 cm2 s−1, K=2.1×108 cm3 mol−1, s=3.9. The overall results suggest that Hoechst 33258 binds tightly to the minor groove of fsDNA and covers four base pairs.