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Background. The patients who require major lower limb joint replacement have a high prevalence of comorbidities, particularly hypertension. Also, they are mostly elderly, with concomitant obesity, chronic kidney disease, poor glycemic control, etc. These factors individually and collectively increase the risk of complications in the perioperative period. Aim. To study the features of preoperative drug therapy for cardiovascular diseases considering the indications in patients hospitalized for elective major lower limb joint replacement. Materials and methods. The study included an unselected sample of patients, 41 males and 77 females (n=118). The mean age was 64.711.1 years, and the mean body mass index was 30.74.9 kg/m2. All patients had a detailed history, an examination with blood pressure measurement, and their therapy for cardiovascular diseases was analyzed. Of 118 patients, 88 (74.6%) had hypertension, 68 (77.2%) received angiotensin-converting enzyme inhibitors, 20 (22.8%) angiotensin receptor blockers, 17 (19.3%) calcium channel blockers combined with other antihypertensive drugs, 11 (12.5%) had no antihypertensive therapy before hospitalization, despite a recorded high blood pressure for several years. Results. The routine preoperative outpatient examination included lipid profile in 77 (65.3%) patients, most of whom had total cholesterol only (66 had levels above normal); low-density lipoprotein levels were measured in only 14 (11.9%) patients, and 9 had levels above target for the relevant risk group. Statins were previously prescribed to only 18 (15.3%) patients. Twenty-two (18.6%) patients reported taking acetylsalicylic acid, with only 7 according to indications; 5 (4.2%) received clopidogrel, of which 1 patient had no indications. Conclusion. Most of the study patients had hypertension of varying severity. Some patients received suboptimal antihypertensive therapy and, in some cases, previously prescribed antihypertensive drug combinations not complying with relevant guidelines. Most patients with hypercholesterolemia did not receive statins with no rationale for not prescribing this class of drugs. Some patients received antiplatelet agents with no appropriate indications.

Aortic dissection is a longitudinal rupture of the aorta associated with the destruction of the middle layer of the aortic wall, the separation of intima and adventitia and the formation of two lumens, which is an urgent condition requiring emergency surgical care. The presented illustrated literature review is devoted to the differentiation of true and false lumens in the acute and chronic stages of aortic dissection during computed tomography. The article summarizes and systematizes the experience of computed tomography diagnostics in aortic dissection based on data available in domestic and foreign publications, as well as the results of their own clinical observations. We have identified the main and secondary signs detected by computed tomography in cases of aortic dissection. The most important and unambiguously indicative of false lumen in classical acute and chronic aortic dissection are the larger size of the false lumen, the sign of a beak and the sign of a web. Other signs, such as calcification of the aorta, changes in the curvature and thickness of the intimal medial flap, the Mercedes-Benz symptom, the presence of thrombotic masses, the characteristics of the interposition of the lumens and the wind indicator symptom are less constant, however, a careful analysis of the totality of all secondary signs allows you to give important diagnostic information and characterize as fully as possible the manifestations significant for a cardiovascular surgeon dissections of the aorta. A cumulative analysis of computed tomographic signs in cases of aortic dissection will help a practicing radiologist not only distinguish the true lumen from the false aorta, but also determine the stage of the process based on their characteristics, which affects the tactics of complex treatment, especially with atypical dissection.

The presented article is devoted to the issue of diagnosis of rupture of ovarian cyst complicated by hemoperitoneum. Ovarian apoplexy ranks third in the structure of urgent diseases in gynecology and second among the causes of intra-abdominal bleeding. It is a sudden hemorrhage into the ovarian tissue, accompanied by a violation of the integrity of its tissue and in some cases bleeding into the abdominal cavity, may be asymptomatic or accompanied by the sudden appearance of unilateral pain in the lower abdomen. In the conditions of emergency rest during emergency diagnostics, the main advantage of ultrasound is the ability to perform in any conditions and in any condition of the patient, therefore, this method is considered in the scientific literature as the main one for the initial examination of such patients, nevertheless, in the scientific literature there is information about the differential diagnosis of emergency gynecological conditions accompanied by hemoperitoneum by X-ray computed tomography. The article presents the signs detected during ultrasound diagnostics and computed tomography in case of rupture of an ovarian cyst, systematized on the basis of literature data and our clinical experience. The main ultrasound and CT symptoms are intraperitoneal effusion with the presence of a sentinel thrombus in the injured ovary and cystic formation in the ovary. The combined analysis of these signs will help the practitioner in an urgent situation not only to determine the blood in the abdominal cavity, but also to determine the source of bleeding, as well as to differentiate the rupture of the ovarian cyst from other conditions accompanied by acute abdominal pain syndrome.

Introduction. In gas industry, predicting sand erosion damage of pipelines by sand particles is a difficult task because this process is strongly influenced by many factors. Particulate matter carried away by the multiphase product during production can seriously compromise the integrity of fluid handling structures such as pipelines and elbows. Materials and methods. A three-dimensional numerical modelling approach in three-phase gas – liquid – solid flows was used to analyze erosion processes in pipe elbows. A key advantage of using CFD is that it can provide a wealth of information such as the effect of various parameters on erosion, maximum erosion rate, and erosion-prone areas. A variation of the Euler method for multiphase fluids was used to simulate three-dimensional unsteady gas-water annular flows with the presence of sand particles. Results. The results showed that a vertical pipe with two elbows is more prone to erosion at the second elbow than atthe first elbow. However, in a horizontal pipe with two elbows, it can be concluded that the first elbow is more proneto erosion. On the other hand, erosion at L/D = 0 showed the maximum erosion rate among all the geometries studied. Conclusions. In a vertical pipe, the second elbow of the pipeline is more susceptible to erosion. In addition, the erosion rate at the second elbow of vertical and horizontal pipes is the same for all studied geometries. The pipeline with L/D = 10 showed the optimal layout choice for vertical and horizontal pipe with an average erosion rate of 57.9 mm/year, which representsthe lowest erosion rate among the options studied.

The article is devoted to the training of a surgeon one of the most complex, painstaking and costly types of education. From the point of view of deontology and the legal framework, students do not have the opportunity to perform various manipulations on patients or participate in operations. In this regard, there is a problem of hyposkillia (lack of practical skills). Clinical skills include not just performing manipulations, but mastering clinical thinking as a special form of human knowledge to comprehend the nature of the disease, mental activity to combine clinical observations, paraclinical studies and personal practical experience. Some ways of solving this problem are proposed, which will improve the training of a competent surgical specialist.

The relationship between the incidence of COVID-19 in pregnant women who have had a coronavirus infection at different gestational ages and the health status of paired neonates is of great interest. However, no sufficient convincing data fully reflecting features of subsequent neonatal period, the state of the immune system in this category of children, affecting characteristics of postnatal period have been accumulated. Based on this, it underlies the relevance of the current study aimed at investigating parameters of clinical and immunological state of neonatal health after paired mothers recovered from COVID-19 at different gestational ages. The prospective study included 131 women and 132 children. The main group consisted of women (n = 61) who had COVID-19 during pregnancy and paired newborns (n = 62) at gestational age (GA) of 3741 weeks, the comparison group women without laboratory-confirmed COVID-19 during pregnancy (n = 70) and paired newborns (n = 70) of similar gestational age. While analyzing the anamnesis of the patients, no significant differences in somatic and obstetric-gynecological diseases were found. Analyzing course of pregnancy revealed that low molecular weight heparins were significantly more often applied in the main group. The term and frequency of delivery by caesarean section in pregnant women in the main group did not significant differ from that of the control group. No significant difference in the frequency of causes accounting for the severity of the condition of neonates in paired mothers with COVID-19 at different trimester of gestation was found. Investigating lymphocyte subset composition, neutrophil phagocytic activity, and IgG class antibodies specific to SARS-CoV-2 was carried out. It was found that lymphocyte subset profile in newborns from paired mothers with COVID-19 at different trimesters of gestation differed only in the level of NK cells (CD56+) in children born to mothers recovered from COVID-19 in the first trimester. In this study, in general, no severe perinatal outcomes in newborns from paired mothers with COVID-19 during pregnancy were documented. No cases of moderate or severe maternal COVID-19 were observed. Therefore, further prospective studies are needed to assess an impact of COVID-19 severity on maternal and fetal birth outcomes and clarify optimal management of pregnant women in such cases.

The essay refutes the idea of M. Haidegger that he will be understood in two hundred years. Heideggers texts are quite monotonous in style; at the same time, they are distinguished by a deep rootedness in the little-known intricacies of Aristotelian thought, on the one hand, and concern for the state of modern science, on the other hand. Heidegger deliberately combines the culture of ancient philosophical thinking with the erudition of philological and physical and mathematical sciences, which sometimes requires a share of irony from the reader in the presence of his own competencies.

Introduction. The use of microorganisms in biotechnological enterprises, agriculture, and forestry can be accompanied by microbial contamination of the environment and have an adverse effect on the human health. The purpose of the research is to develop and put into practice quantitative criteria for the pathogenicity of biotechnological microorganisms as the first stage in assessing their safety. Materials and methods. Lethal dose (LD50) and “threshold” (Limbact) doses, dissemination in internal organs, toxigenicity, and “pathogenicity enzymes” of thirty-three strains of saprophytic, opportunistic and pathogenic bacteria, 250 strains of 10 genera proposed for use in biotechnology, and 11 strains already used as active biopreparation substances were investigated. Results. Based on the criteria for primary sanitary and hygienic assessment, microorganisms were selected into three groups: strains recommended for industrial use; strains at risk of application; strains not recommended for use. Among the studied 250 strains of microorganisms proposed for use in biotechnology, 38.9% did not have pathogenic properties and can be used, 41.0% are conditionally pathogenic and are at risk of use, and 20.1% are not recommended for use in biotechnology due to the presence of pathogenic properties. A study of the pathogenic properties of 11 strains of bacteria that are the part of modern biological products showed that they belong to non-pathogenic microorganisms. Limitations. The development and testing of the criteria for the primary sanitary and hygienic assessment of microorganisms proposed for biotechnology were carried out on a large material (more than 250 strains) with a single exposure to high doses, which did not allow us to assess the specific effects of exposure to strains during long-term exposure and may be the subject of further research. Conclusion. The criteria for the primary sanitary and hygienic assessment of microorganisms proposed for use in biotechnologies include average lethal and “threshold” doses dissemination in internal organs, toxigenicity and activity of “pathogenicity enzymes”. All planned for use in biotechnology microorganisms should be examined for possible pathogenic properties. Biological products used in real time in the national economy contain only saprophytic microorganisms as an active substance. The problem of biosafety should be solved in the direction of studying the specific effect of microorganisms and biological products on the immune system influence and the state of the microcenosis of the body.

Dega (Dmitriy) Vitalyevich Deopik (born in 1932) is a Soviet and Russian archaeologist, historian and orientalist. Born in Moscow, in the family of a Russian painter and graphics artists Vitaliy Nikolaevich Goriaev (1910-1982) and his wife Valentina Pavlovna Deopik (1907-1990) who herself was an outstanding Soviet painter and artist. Graduated from the MSU Faculty of History in 1956. In 1996 obtained Doctor of Sciences degree. In 2000 was one of the founders of Faculty of History of St. Tikhons Orthodox University. For many years was head of or participated in archaeological expeditions in the regions adjacent to Caucasus and in Black Sea Region. His areas of academic interest include: quantitative methods in historical studies, Biblical archaeology, archaeology of Southern Russia, traditional history of Vietnam, Cambodia, Burma, Indonesia, China, problems of East Asia archaeology and ancient East Asia writing systems. He created Russian school of quantitative and precise methods in sinology.

Infectious diseases, due to their epidemic potential, are capable of global spread, they are unpredictable, and their effective control is possible only on a planetary scale. In no other field of medicine, such radical and often unpredictable changes occur in a short time in the form of the elimination of individual diseases, the identification of a large number of previously unknown and the return of already forgotten diseases, a significant change in the pathogenesis and clinical symptoms of a significant number of them. Under these conditions, these changes often create problems for the health authorities and the public, ahead of their ability to recognize and respond in a timely manner to the emergence of epidemics and the transformation of pathological manifestations. Significant recent scientific progress has only slightly clarified our knowledge of the biological and genetic diversity of pathogens. Until now, the entire spectrum of infectious pathology remains unclear, and its numerous manifestations often take even experienced specialists by surprise. Evidence of our limited knowledge and ability to control the situation is the ongoing COVID-19 pandemic for three years now, the increasingly frequent detection of new infections, the return of infections declared defeated, evidence of the role of infectious factors in the etiology of many other human diseases.

Aim. To study relationship between anemia and health-related quality of life (HRQOL) of chronic kidney disease stage 5 patients (CKD) undergoing renal replacement therapy by programmed hemodialysis. Materials and Methods. The study was conducted on the basis of dialysis centers/departments in Moscow. The Russian-language version of the KDQOL-SFTM questionnaire, validated in Russia, was used to assess the HRQOL of patients on hemodialysis. A total of 723 patients were interviewed. Hemoglobin (Hb) levels were assessed in 442 patients based on outpatient records. To identify the relationship between Hb level and HRQOL scales, a correlation analysis was performed, as well as a HRQOL analysis in the three groups differing in hemoglobin levels (100; 100-120 and 120 g/l). Statistical data processing was carried by SPSS.22 and using parametric and nonparametric statistical methods. Results. Correlation relationship was revealed between Hb and HRQOL of patients on hemodialysis on the scales "symptoms/problems", "pain", "vital activity, energy" and "total physical component of health". According to the scales "symptoms/problems" and "vital activity, energy", higher rates were noted among patients with Hb higher than the target level, which confirms the recommendations concerning the possibility of achieving higher Hb among individual patients who have not serious concomitant diseases and cardiovascular complications in order to improve their quality of life. Conclusion. HRQOL assessment is important tool for planning and evaluating the effectiveness of anemia drug therapy among patients with CKD on hemodialysis.

Dy3+-doped, Ag and Dy3+-co-doped, and Tm3+ and Dy3+-co-doped oxyfluoride glasses have been prepared by a conventional melt-quenching method. White and yellowish light emissions were generated in the glasses under excitation in the range from 350 to 390 nm. Changes in the excitation wavelength in the ultraviolet (UV) range do not significantly alter the emission tint of the Dy and Ag and Dy-co-doped glasses. On the contrary, the tint of the Tm and Dy co-doped glasses considerably varies under excitation in the same range.

The article presents the cultural genesis of the nobility society through the synthesis of the military adventure of men and the playing leisure of women into a common courtly culture In the courtly culture of court society, a system of regulation of relations arises with an orientation to an excellent act and, accordingly, with the requirement to see yourself from the outside, show yourself from the best side. As a result, a phenomenon arises that can be called pictorial Pictorial communication precedes the emergence of the visual arts, which sometimes arise on a random occasion. Ancient painting was no exception. The turn to luxury after the Greco-Persian wars initiated the mural by analogy with the trellis art of the Persians. The content of ancient painting in the form of wall painting and vase painting was the culture of communication that Hegel in his lectures on aesthetics called the color of man and which by the 19th century began to disappear.

The warming is global problem. In natural environments, heat stress is usually accompanied by drought. Under drought conditions, water content decreases in both soil and air; yet,the effect of lower air humidity remains obscure. We supplied maize and barley plants with an unlimited source of water for the root uptake and studied the effect of relative air humidity under heat stress. Young plants were subjected for 48 h to several degrees of heat stress: moderate (37 °C), genuine (42 °C), and nearly lethal (46 °C). The conditions of lower air humidity decreased the photochemical activities of photosystem I and photosystem II. The small effect was revealed in the control (24 °C). Elevating temperature to 37 °C and 42 °C increased the relative activities of both photosystems; the photosystem II was activated more. Probably, this is why the effect of air humidity disappeared at 37 °C; the small inhibiting effect was observed at 42 °C. At 46 °C, lower air humidity substantially magnified the inhibitory effect of heat. As a result, the maximal and relative activities of both photosystems decreased in maize and barley; the photosystem II was inhibited more. Under the conditions of 46 °C at lower air humidity, the plant growth was greatly reduced. Maize plants increased water uptake by roots and survived; barley plants were unable to increase water uptake and died. Therefore, air humidity is an important component of environmental heat stress influencing activities of photosystem I and photosystem II and thereby plant growth and viability under severe stress conditions.

In liquid crystal/quantum dots composites, a nonmonotonic increase in the electrooptical performance with a change in dopant concentration was found. The changes are explained within the framework of the self-organization hypothesis of quantum dots.

The excitation of proflavine dimers in aqueous solution was analyzed using various functionals and dispersion corrections.

Cyclometalation of 2-arylbenzimidazoles decorated with various polyaromatic fragments leads to formation of strongly absorbing iridium dyes applicable in DSSC and enables access to structurally diverse iridium(iii) complexes.

Light-induced magnetization response unfolding on a temporal scale down to femtoseconds presents a way to convey information via spin manipulation.

An intriguing hybrid magnetic configuration Γ4+3 with non-zero spontaneous magnetization along b-axis was found in DNFO91 single crystal. Contributions of R3+ were distinguished and the relative strength can be manipulated with magnetic field.

Background. Psoriasis is a systemic chronic immune-mediated inflammatory skin disease of a multifactorial nature that affects about 2 to 3% of the population worldwide. Given the systemic nature of the disease, the level of comorbid diseases is high, especially those associated with damage to the cardiovascular system. According to the results of various meta-analyzes, it has been proven that among patients with psoriasis, the risk of developing hypertension is high. Aim. To evaluate the prevalence of arterial hypertension (AH) among patients with psoriasis. Materials and methods. We present an open, prospective, observational clinical trial, which includes 60 patients diagnosed with psoriasis vulgaris of varying severity and psoriatic arthritis. All patients underwent clinical and laboratory tests, including the level of total cholesterol, glucose, creatinine, C-reactive protein, calculation of glomerular filtration rate. Body mass index, blood pressure level according to the Korotkov method, severity of the disease by determining psoriasis area and severity Index (PASI), dermatological quality of life index (DLQI), levels of hospital anxiety and depression according to the HADS scale were also assessed. The SCORE2 scale was used to assess cardiovascular risk in patients aged 40 to 69 years, the SCORE 2OP scale for patients aged 70 to 89 years, respectively. Cardiovascular risk was defined as low for patients under 40 years of age. All study participants were examined for the presence of AH. Results. The prevalence of hypertension was 25%, with prevalence among men (15%). The prevalence of hypertension is directly proportional to the severity of psoriasis (13% with hypertension in patients with severe psoriasis, 12% with hypertension with moderate psoriasis, 2% with hypertension with mild psoriasis). Conclusion. Given the high prevalence of AH among patients with psoriasis, it is necessary to form a multidisciplinary approach to the treatment of patients, including a thorough assessment of cardiovascular risk in clinical practice.

Aim. To evaluate the course of resistant arterial hypertension in patients with visceral obesity, to identify predictors of unsatisfactory shot-term and long-term treatment outcomes, to optimize therapy and improve adherence to treatment. Materials and methods. A total number of 90 individuals with a history of refractory or resistant arterial hypertension and visceral obesity were a subject of intensive study. The prospective analysis group consisted of 30 patients with an individualized management plan each, whereas the retrospective group of real clinical practice included 60 participants. At baseline, all patients were taking antihypertensives like ACE inhibitors or angiotensin II receptor blockers (ARBs)/angiotensin-converting enzyme inhibitors, calcium channel blockers (CCBs), and a diuretic. After the initial examination, therapy was individually optimized for each patient in accordance with current clinical guidelines. Most patients in the retrospective group received ARBs valsartan or losartan, CCBs amlodipine, the diuretics indapamide and torasemide, the -blockers bisoprolol and metoprolol, the 2-agonist moxonidine, and the mineralocorticoid receptor antagonist spironolactone. Patients in the prospective group were prescribed ARBs telmisartan and azilsartan, the CCB lercanidipine, thiazide and thiazide-like diuretics indapamide and chlorthalidone, the -blockers nebivolol and carvedilol, the 1-blocker doxazosin, and spironolactone. A re-examination was performed 2 months later. Subsequently, regular communication was maintained with participants of the prospective group during 8 months using a messenger. Communication with patients of the retrospective group was not maintained. All the patients were then asked to self-report their health status by conducting a telephone survey. Results. After 2 months, according to the data of the follow-up, in the retrospective group the target values of mean daily SBP and DBP were observed in 35 and 36.7% of patients, though the statistics among the patients in the prospective group were 66.7 and 60%, respectively. After 10 months, according to the results of the interviews, the target values of SBP and DBP were observed in 10 and 18.3% of patients, though the statistics among the patients in the prospective group were 93.3 and 96.7%, respectively. In the retrospective group, 78.3% of patients changed the previously selected therapy, in the prospective group this figure was only 20%. In the retrospective group, anthropometric data did not change, while in the prospective group, weight and waist circumference significantly decreased (p0.05). Conclusion. Maintaining regular contact with patients and a well-rounded treatment strategy with individualized choice and dosage of medications with an emphasis on modern metabolically neutral drugs with a prolonged duration of action led to better BP control, increased adherence to therapy and indicated significant weight loss among the patients from the prospective group.

Aim. To study the incidence of contrast-associated acute kidney injury (CA-AKI) in patients with various forms of the acute coronary syndrome (ACS). Materials and methods. An observational, open, single-center, prospective study included 83 patients with ACS with indications for percutaneous coronary intervention. Of these, 40 were included in the ST-segment elevation ACS group, and 43 patients were included in the non-ST-segment elevation ACS group. The study is registered at ClinicalTrials.gov as NCT04163484. Before the intervention, all patients had a general examination, ECG, complete blood count, blood chemistry, and blood coagulation tests. Later, 24 and 48 hours after the intervention, the serum creatinine (SC) trend was assessed. The primary endpoint was the development of in-hospital CA-AKI according to KDIGO criteria within 72 h or before hospital discharge. Results. An increase in SC level by 25% from the baseline was observed in 6 patients in each subgroup of ACS. A marked increase in SC by 50% and a decrease in glomerular filtration rate were reported only in 2 patients (one in each subgroup of ACS). Conclusion. The study showed that the incidence of CA-AKI in patients with ACS with ST-segment elevation was 2.3%, and in the group of patients with ACS without ST-segment elevation 2.5%.

The formation of the global media space contributes to the active penetration of a different, non-Russian, culture in the domestic media, with one or another broadcast lifestyle having a great impact on the behaviour of young people, shaping, among other things, their oral and written speech through slang. Slang is a rather actively developing lexical layer, new slang units appear in the language every day, they reflect not only cultural values, priorities and aspirations of those who use them, but also demonstrate specific ways of shaping thinking. The study of American slang words and their influence on contemporary Russian youth is especially relevant in the context of information warfare. The authors examine slang words that are reflected in the Russian-language media and social networks, as well as the peculiarities of their influence on the cultural values of adolescents. The materials with slang words, posted from January 2019 to February 2022 in social networks TikTok, VKontakte, on the youth Internet portals QRU, Spletnik, Elle Girl were selected by the method of continuous sampling. The authors reveal their meaning in the context of the analysed publications and come to the conclusion that the active use of such slang units as, for example, crash, cheater, flex, ROFL indicates the priority of idleness in the leisure time of young people. At the same time, teenagers show a lack of interest in cultural leisure: there are no slang expressions for such activities as going to a museum, sightseeing, theatre, concert hall or library.

There is no doubt that there is a similar genetic predisposition in close relatives to the development of cardiovascular disease, while the features of coronary anatomy and possible commonality of pathological process in close relatives remain beyond research interest. Aim. To study the anatomy of the coronary arteries, the nature and severity of coronary lesion in pairs of male siblings suffering from coronary artery disease, in comparison with control pairs of patients who are not closely related. Materials and methods. This retrospective study included 87 male patients. The main group (58 patients or 29 couples) consisted of siblings with a horizontal hereditary burden for coronary artery disease. The control subgroup included 29 big brothers of the main group and another 29 patients who unrelated to them. To assess the degree of anatomy identity of the coronary bed in pairs of patients, we used a comparative characteristic of 25 segments of the coronary arteries (SyntaxScore). If there was similarity in anatomy and in the severity of the atherosclerotic process, each segment was assigned one point. In terms of the ratio of the total scores to the total number of segments (n=25), each pair of patients was assigned anatomical and atherosclerotic identity indexes. Results. According to the results of the study, the average indices of anatomical and atherosclerotic identity indexes were significantly higher in pairs of sibs compared to control pairs of patients (0.92 versus 0.88, p=0.008 and 0.92 versus 0.76, p0.001 respectively). When analyzing atherosclerotic lesions of the coronary arteries in pairs of sibs, a statistically significant coincidence of lesions in a number of segments of the coronary arteries was revealed, with the highest agreement in the left main coronary artery (=0.869, p0.001) and in the proximal segments of the main branches of the coronary arteries: left anterior descending artery (=0.786, p0.001) and right coronary artery (=0.812, p0.001). In the group of control pairs, such regularities were not revealed. Conclusion. In siblings with horizontal hereditary burden for coronary artery disease along the sibling line, the average anatomical and atherosclerotic identity indexes are significantly higher compared to control pairs. In pairs of siblings, there is the highest and most reliable probability of developing coronary atherosclerosis in the left main coronary artery and in the proximal segments of the left anterior descending artery and right coronary artery.

Introduction. The impact of a complex chemical load on the population of the whole world, the use of pharmacological preparations, alcohol-containing products are the main factors that determine the high frequency and prevalence of toxic hepatitis. The search for new approaches for the treatment of toxic liver damage in order to restore structural and functional disorders is one of the urgent tasks of medicine. Of particular interest are the methods of regenerative medicine based on the use of various types of stem cells. Purpose of the study - study of the effect of transplantation of multipotent mesenchymal stem cells on the processes of regeneration in the liver of rats with induced acute toxic hepatitis. Materials and methods. An experimental study was conducted on 114 outbred male rats (3-5 months old) weighing 250-390 g. The animals were divided into 3 main groups. Group 1 - control animals (n=15). Group 2 (positive control) single intragastric injection of an oil solution of carbon tetrachloride (CCl4) at a dose of 1500 mg/kg (n=41); Group 3 (n=58) intragastrically injected with CCl4 at a dose of 1500 mg/kg, and intravenous transplantation of MMSC of 2×106, was carried out. On the 1st, 3rd, 5th, 7th day of the experiment as well as the collection of organs and tissues for histological and morphometric studies was made. Results. According to immunofluorescent analysis, intravenously transplanted MMSCs were found in the liver of rats at the 3rd and all subsequent periods of observation. In the present study, MMSC transplantation led to a statistically significant decrease in infiltrative processes in the liver tissue on the 3rd and 5th days of the study by 28.3% (p

In the southern regions of Russia, high risks of the spread of malaria remain. The situation is aggravated by the increase in average annual temperatures in the long-term distribution and the growth of eutrophicated small water bodies, which are favorable for the completion of the life cycle of the disease vectors mosquitoes of the genus Anopheles. In order to establish the relationship between climatic factors and the status of populations of insect vectors of the causative agent of malaria, an analysis of the amount of surface water was carried out using GIS technologies. For the calculation, the MNDWI index was chosen, based on the use of images in the Green and SWIR bands of the Landsat 8 satellite. The indicators of the average and maximum number of larvae and adults of mosquitoes were analyzed on the basis of information presented in the annual reports of the Office of the Federal Service for Supervision of Consumer Rights Protection and human well-being in the Volgograd region. There are three malariogenic zones in the region: northern (Kamyshinsky), central (Volgograd) and southern (Kotelnikovskaya). The overall dynamics of MNDWI for the Kamyshin zone tended to increase the water index from -0.176 to -0.171 in a two-year range. Similar MNDWI indicators for the Volgograd and Kotelnikovsky malariogenic foci also had a positive trend in the period from 2018 to 2020. The modified normalized water index for them increased from -0.152 to -0.126 and from -0.215 to -0.158, respectively. Calculation of the Pearson correlation coefficient for the study area revealed the presence of a high closeness of a direct relationship between the MNDWI index and the maximum number of larvae/adults in the Kamyshin region (0.994/0.833). For Volgograd, the strength of the correlation was in the region of average (noticeable) values, except for the average value for adults, where the relationship was strong (0.904). In Kotelnikovo, the relationship between periods of drought and the number of larvae was inversely proportional, with the exception of the maximum number of adults (0.943), which can be explained by the small value of the sample of the final emergence of insects and the measurement error due to the logic of the methodology for studying the number of larval forms in natural water bodies. The results obtained can be used to improve the system for monitoring the dynamics of malariogenicity by supervisory authorities in the field of population welfare. Further research prospects are associated with a change in approaches to assessing the average values of the number of malaria vector insects, as well as with our own field work on the study of the composition of the mosquito entemofauna of the river. Anopheles for the purpose of calibrating data reported by government agencies.

Numerous high-elevation alpine plants of the Qinghai-Tibet Plateau (QTP) also have disjunct distribution in adjacent low-altitude mountains. The out-of-QTP versus into-the-QTP hypothesis of alpine plants provide strong evidence for the highly disputed assumption of the massive ice sheet developed in the central plateau during the Last Glacial Maximum (LGM). In this study, we sequenced the genomes of most known populations of Megadenia, a monospecific alpine genus of Brassicaceae distributed primarily in the QTP, though rarely found in adjacent low-elevation mountains of north China and Russia (NC-R). All sequenced samples clustered into four geographic genetic groups: one pair was in the QTP and another was in NC-R. The latter pair is nested within the former, and these findings support the out-of-QTP hypothesis. Dating the four genetic groups and niche distribution suggested that Megadenia migrated out of the QTP to adjacent regions during the LGM. The NC-R group showed a decrease in the effective population sizes. In addition, the genes with high genetic divergences in the QTP group were mainly involved in habitat adaptations during low-altitude colonization. These findings reject the hypothesis of development massive ice sheets, and support glacial survival of alpine plants within, as well as further migration out of, the QTP.

Intra-specific body size variation in ground beetles is studied insufficiently, especially in response to climatic factors. Even less studied is the sexual dimorphism (hereinafter referred to as the SSD), its geographic variation patterns and response to climatic factors. We sampled ground beetles Pterostichus melanarius in 15 regions of Northern Eurasia along latitude and longitude gradients (in 17 degrees and 121 degrees, respectively), including differing habitats (open and forested) in the spectrum of anthropogenic impact (cities, suburbs, arable lands and natural). 7677 specimens were measured by six morphometric traits – elytra, pronotum, head length and width, distance between eyes. Our software applied made it possible both to catch the smallest changes in the size of traits in females and males, and to determine their direction. Temperature related factors mostly reduced beetles traits values, but precipitation related factors – enlarged them. Elytra and pronotum parameters were the traits which response differently in males and females to climatic factors, these traits showed more pronounced SSD. Head parameters showed SSD in response to those factors too. That response had the similar direction and was expressed more, either in females or males. The latter processes implemented to a greater extent in relation to the temperature including bioclimatic factors.

The dark-detached leaves are widely used as a model to investigate senescence-delaying mechanisms of cytokinin (СК). The role of CK during senescence of detached Arabidopsis leaves in the light is less examined, although they provide unique opportunities for the study of the interaction of CK and the photosynthetic apparatus. Within the first four days of incubation of the leaves on water photosynthesis activity, chloroplast gene expression and chlorophyll content declined in all genotypes with a functioning CK receptor AHK3, while anthocyanin level and expression of its regulatory and biosynthesis genes increased. Application of exogenous CK visibly retarded the senescence symptoms but decreased photosystem II function, anthocyanin accumulation and promoted ROS production. The hormone stimulated starch accumulation in the chloroplasts and altered the expression of the gene for the extracellular cell wall invertase CWINV1. Although the AHK3 receptor played the most important role in the CK action, we also observed specific effects in loss-of function mutants for phosphortransmitters and type B response regulators. The results suggest that CK action in detached Arabidopsis leaves in the light is superimposed on modified carbon fluxes and starch turnover which results in an intricate combination of stress-related and senescence inhibiting effects.

BACKGROUND: Despite the development of new methods for treatment of pterygium, the frequency of its recurrence ranges from 5 to 89%. AIM: Was to evaluate the effectiveness of ciclosporin for prevention of pterygium recurrence. MATERIALS AND METHODS: Under follow-up, there were 104 patients operated for pterygium, of which 26 had a high risk of recurrence, they were divided into 2 groups: the 1st group 13 patients received 0.05% ciclosporin in addition to basic therapy; the 2nd group 13 patients receiving only basic therapy. Patients of studied groups were under dynamic observation for 1 year. RESULTS: After 3 months, all patients of the 1st group showed normalization of the osmolarity of the lacrimal fluid (OLF) and the ocular surface disease index (OSDI). In patients of the 2nd group, elevated values of OLF and OSDI remained characterizing the persistence of an inflammatory reaction signs and a high risk of the disease recurrence, as well as the formation of a hypertrophic scar in 38.5% of cases. In one year after surgery, 3 patients of group 2 had a recurrent pterygium, which required surgical retreatment. CONCLUSIONS: We obtained results showing a positive effect of ciclosporin use on dynamics of inflammatory reactions of the ocular surface in postoperative period, which made it possible to prevent pterygium recurrence after its surgical treatment in patients with high risk of recurrence.

Glioblastoma (GBM) is characterized by exceptionally high intratumoral heterogeneity. However, the molecular mechanisms underlying the origin of different GBM cell populations remain unclear. Here, we found that the compositions of ribosomes of GBM cells in the tumour core and edge differ due to alternative RNA splicing. The acidic pH in the core switches before messenger RNA splicing of the ribosomal gene RPL22L1 towards the RPL22L1b isoform. This allows cells to survive acidosis, increases stemness and correlates with worse patient outcome. Mechanistically, RPL22L1b promotes RNA splicing by interacting with lncMALAT1 in the nucleus and inducing its degradation. Contrarily, in the tumour edge region, RPL22L1a interacts with ribosomes in the cytoplasm and upregulates the translation of multiple messenger RNAs including TP53. We found that the RPL22L1 isoform switch is regulated by SRSF4 and identified a compound that inhibits this process and decreases tumour growth. These findings demonstrate how distinct GBM cell populations arise during tumour growth. Targeting this mechanism may decrease GBM heterogeneity and facilitate therapy.

The economic development of the State depends on the work of the able-bodied population. The cardiovascular system is highly reactive and reacts to many harmful production factors: noise, vibration, ionizing radiation, etc. Spastic vascular changes are the precursors of peripheral angiodistonic syndrome, which is part of the symptom complex of vibration disease, and accompanies polyneuropathy from physical overstrain. Detection of the disease at the initial stage of development for the appointment of timely prevention and treatment is the main task of modern medicine. Ultrasound examination, as one of the imaging methods in radiation diagnostics, successfully copes with this problem. The study aims to develop criteria for early diagnosis of occupational pathology of the vessels of the upper extremities in workers of machine-building production. We have examined 126 workers of the machine-building plant using standard clinical and laboratory methods, ultrasound examination of the vessels of the upper extremities and X-ray examination of the hands. The main harmful production factors in the workplace were local and general vibration, the severity of the labor process, noise. The severity of the labor process of at least class 3.1 with a work experience of 21 years contributed to the development of spastic changes in the vessels of the upper extremities in power engineering workers. Patients complained in 25% of cases, although an objective examination by a neurologist revealed no pathological changes characteristic of the pathology of the vascular bed. There were radiological changes in the hands in 83% of the examined workers and manifested as a violation of local circulation: cyst-like rearrangement in the bones of the wrists were in 40% of cases, in the form of periarticular osteopenia - in 70% of cases. The most informative method of early diagnosis of vascular changes in the main arteries of the upper extremities is ultrasound. At the same time, the characteristic signs are an increase in the resistance index with the preservation of speed indicators (recorded in 100% of cases), a violation of venous outflow in 82%, pathological vascular tortuosity in 76%. Ethics. The Local Ethics Committee of the North-Western Scientific Center of Hygiene and Public Health has approved the study, Protocol No. 23.1 of 10/28/2020.

Introduction. The cardiovascular system reacts to almost all occupational hazards. Various methods for diagnostics, in particular ultrasound, may help to identify preclinical forms of the disease. The study aims to development of criteria for early diagnosis of occupational diseases of the vessels of the upper extremities in machine-building workers. Materials and methods. One hundred twenty-six workers of a machine-building plant were examined using standard clinical and laboratory methods, ultrasound examination of the vessels of the upper extremities and X-ray examination of the hands. Results. Laboring job, hand-arm and whole-body vibration, and noise with an average work experience of 21 years are risk factors for spastic changes in the vessels of the upper extremities in workers of machine-building production. X-ray changes in the hands are determined in 83% of cases and manifest themselves as a violation of local blood circulation, cystic restructuring in the bones of the wrists, and peri-articular osteopenia. Characteristic signs of vascular pathology detected by ultrasound are an increase in the resistance index with the preservation of speed indices (in 100%), impaired venous outflow (in 82%), and pathological tortuosity of blood vessels (in 76%). Limitations. We did not standardize workers depending on tobacco smoking due to the low number of workers in certain occupations. Conclusion. The results of ultrasound examination of the main vessels of the upper extremities in power engineering workers showed the presence of spastic vascular changes, which, with further exposure to occupational hazards, develop into angiodystonic syndrome, which is one of the syndromes of effects of vibration or polyneuropathy.

Introduction. Chronic viral hepatitis C (CHC) is a ubiquitous infectious disease, a significant limitation of which WHO attributes to the use of a new highly effective antiviral therapy. Previously, two B-cell epitopes were identified in NS4a antigen of the hepatitis C virus (HCV). It was shown that certain titers of antibodies (ABs) to the extended C-terminal epitope (16871718 a.a.) can predict a high probability of achieving a sustained virological response (SVR) to standard therapy with pegylated interferon- and ribavirin. The aim of the work was to determine immunoreactivity of two B-cell epitopes (middle and C-terminal) of NS4a antigen, and to estimate a possible association of ABs to them with the achievement of SVR after standard interferon therapy and treatment with direct antiviral drugs (DAAs) daclatasvir and sofosbuvir (velpanat). Materials and methods. Blood serum samples of patients with CHC (n = 113), of which 55 participants received standard interferon therapy, 50 received velpanate treatment, the remaining 8 received no therapy were examined. The middle B-cell epitope (positions 2434 a.a.) of NS4a was synthesized by the solid-phase method, while the C-terminal epitope (3454 a.a.) was obtained using genetically engineered techniques. Enzyme immunoassay (ELISA) testing of the sera collected before treatment was performed for the two selected epitopes according to the conventional methods. Results. The antibodies to the C-terminal epitope were detected significantly more frequently than those to the middle one (p = 0.01) when analyzing the blood sera of patients (n = 113). The presence of ABs to the C-terminal epitope in the serum samples of participants who completed standard interferon therapy was associated with the achievement of SVR (p = 0.0245). In the blood sera of participants who completed therapy with velpanate, an association of the presence of ABs to the C-terminal epitope with the achievement of SVR was also established (p 0.0001). The presence of ABs to the middle B epitope was not associated with the achievement of SVR, regardless of the therapy used. Discussion. The observed difference in the immunoreactivity of the two B-cell determinants may be associated with the localization of the nearest Th-epitopes, the sensitivity of NS4a antigen to proteolytic enzymes, and the peculiarities of epitope presentation by antigen-presenting cells. However, it should be noted that the immunoreactivity of the middle B-epitope is poorly studied. Although the association of ABs to the C-terminal epitope with the achievement of SVR has been shown by several scientific teams, the detailed molecular mechanism of their influence on the effectiveness of therapy is unclear. Conclusion. In CHC, ABs to the C-terminal epitope of NS4a are produced more frequently than those to the median epitope. The presence of ABs to the C-terminal epitope is a predictive marker of a high probability of achieving SVR, regardless of the type of therapy and antibody titer.

Background: Hepatoprotectors, currently used in clinical practice, often show low efficacy. One of the promising goals in the therapy of liver diseases is the targeted synthesis of smallmolecule drugs with hepatoprotective activity. Some heterocycles, such as 1,4-dihydropyridines, are proven to be good hepatoprotectors. Objective: This study aimed to prepare and investigate the hepatoprotective effects of some dihydro- and tetrahydro pyridines using the experimental rat model of tetrachloromethane (CCl4)- induced acute liver damage. Methods: Several 1,4-dihydropyridines and 1,2,3,4-tetrahydropyridines were synthesized by the reaction of cyanothioacetamide with carbonyl compounds and active methylene compounds, such as Meldrum’s acid, ethyl cyanoacetate or β-keto esters, followed by S-alkylation of intermediate pyridine-2-thiolates. In silico prediction studies were performed to explore the possible protein targets. Using virtual bioscreening tools, we selected the six most promising compounds from the library of new partially saturated pyridines. Six novel compounds were characterized by IR, 1H, 13C DEPTQ NMR spectroscopy, 2D NMR experiments (1H–13C HSQC, 1H–13C HMBC), HRMS (ESI), and elemental analysis. The hepatoprotective effects were studied in vivo on eighty white rats with CCl4-induced liver damage. Biochemical studies, including thymol turbidity test and measurements of levels of bilirubin, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase in the blood of rats, were performed. Results: Five of six studied compounds showed a good hepatoprotective effect on a rat model of tetrachloromethane (CCl4)-induced acute hepatitis. The blood of rats in the experimental groups revealed significantly lower levels of total bilirubin, alanine aminotransferases, aspartate aminotransferases, thymol turbidity and alkaline phosphatase compared to the control group of rats with acute toxic tetrachloromethane hepatitis. Conclusion: Our results suggests that compound, (benzyl 4-(4-chlorophenyl)-5-cyano-6-({2-[(3,4- dimethylphenyl)amino]-2-oxoethyl}thio)-2-methyl-1,4-dihydropyridine-3-carboxylate), can be considered as candidate for further preclinical studies. Moreover, as per our knowledge, this is the first report on the hepatoprotective effects of the 1,2,3,4-tetrahydropyridine series.