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List of sequences used in the mitochondrial analysis. A detailed table with the information of each sequence used is given. The information comprises the name of the site, country of origin, haplotype, GenBank Accession numbers and bibliographic references. (DOC 362Â kb)

Mantel test correlation between geographical distance (kilometers) and genetic distances (given as FST, FST/(1-FST) or RST) for all sites. (DOC 637Â kb)

Estimated pairwise FST and RST values based on sampling sites. (DOC 53Â kb)

Genetic diversity of the microsatellite loci and sampling sites. Table with the number of alleles, allelic richness, number of private alleles, expected heterozygosity, unbiased expected Neiâ s heterozygosity, observed heterozygosity, inbreeding coefficient and exact P-value for Hardy-Weinberg equilibrium test are listed for the microsatellite loci and the sampling sites. (DOC 140Â kb)

Assignment probability (K between 6 and 9) and assignment probability per cluster, as identified by DAPC analysis. (DOC 35Â kb)

The relative probability of different hypotheses was assessed using approximate Bayes Factors (BF). A generation time of 5.5 for the noble crayfish was used in the analysis. Hypotheses were examined for the past 12,000 and 7000Â years. (DOC 2096Â kb)

Starting values (α, σ, β and τ) for hyperpriors (log(N0), log(N1), log(Θ) and log(T)) for each independent MCMC run. (DOC 30 kb)

Inference of number of clusters. The file contains graphical representations of a) DeltaK for each K (1 to 16) produced by Structure Harvester and b) Bayesian information criterion (BIC) for every number of clusters, using DAPC. (DOC 108Â kb)

Fixation indices for all microsatellite loci. Values of the fixation indices for all microsatellite loci based on infinite allele model (FIT, FIS and FST) and stepwise mutation model (RST). (DOC 30Â kb)

Point estimates with their upper Confidence Interval (CI) for all parameters (log(N0), log(N1), log(Î ) and log(T)) and every genetic cluster for a generation time of 3.5 and 5.5. (DOC 48Â kb)

The modified conditions used for the PCR and amplification of each microsatellite loci are given. (DOC 32Â kb)

Graphical representation of genetic barriers (1 to 10) based on: a) sixteen sampling stations, all microsatellites loci and the genetic distance DCE, b) sixteen sampling stations, all microsatellites loci and the FST, c) sixteen sampling stations, each microsatellite locus (Aas8, Aas766, Aas1198, Aas2498, Aas3040 and Aas3950) and FST, d) nine genetic clusters (of the Structure software), all microsatellites loci and the genetic distance DCE, and e) nine genetic clusters (of the Structure software), all microsatellites loci and the FST. (DOC 10235Â kb)

Graphical representations of the DAPC scatterplots of the first two principal components for K between 6 and 8. (DOC 325Â kb)

Posteriors and priors densities plots for time since the population decline (T) for each genetic cluster (cluster 1 to 9) and the global data set (samples from different demes were merged into one dataset; black color). (DOC 674Â kb)

Map of the sampled noble crayfish in Greece (Copyright Š 2014 Esri and its licensors. All rights reserved) where abbreviation of the sampling sites, names of major rivers, mountains and sierra, type of genetic markers used (mitochondrial and/or nuclear; mtDNA and nDNA, respectively) and elevation are given. (DOC 2355 kb)

Posteriors and priors densities plots for the past (N1) and present (N0) population sizes of each genetic cluster (cluster 1 to 9) and the global data set (samples from different demes were merged into one dataset; black color). (DOC 859Â kb)

Pairwise within and between genetic distances of the haplotype groups (G1 to G6) for COI and 16S. (DOC 56 kb)

Tsaparis, Dimitris, Karaiskou, Nikoleta, Mertzanis, Yorgos, Triantafyllidis, Alexander (2014): Non-invasive genetic study and population monitoring of the brown bear (Ursus arctos) (Mammalia: Ursidae) in Kastoria region - Greece. Journal of Natural History 49 (5): 393-410, DOI: 10.1080/00222933.2013.877992