Search

Zirconia nano particles were synthesized from Zirconium oxychloride octahydrate using the extract of Zingiber officinale by Green synthesis. The synthesis was confirmed by the color change from pale yellow to pink. The synthesized nano particles were characterized by UV-visible spectrophotometer, Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD). The nanoparticles were found to be tetragonal with the XRD results. As an application in Dentistry, the activity of the zirconia nanoparticles were checked using well diffusion method with an oral bacterium, Streptococci mutans, and found the nano particles were inhibiting the growth of the bacterium.

Autism is a Neuro-developmental disability characterized by severe social, communicative and cognitive deficits, resulting in significant lifelong disability. Autism requires long-term treatment, yet, despite the severity of this disorder, some children achieve remarkable long lasting gains. Over the years, many studies have been published on comprehensive treatment approaches that seek to reduce the general level of impairment in autism. An increasing body of empirical research suggests that early, intensive, structured intervention, based on the principles of applied behavior analysis, is effective in remediating the intellectual, linguistic, and adaptive deficits associated with autism. Early intervention programs are indeed beneficial for children with autism, often improving developmental functioning and decreasing maladaptive behaviors and symptom severity at the level of group analysis. To study the efficacy of early intensive behavioral intervention, 40 children were selected and two groups were formed. Written consent was taken from parents. Therapy was provided at the centre for autism spectrum disorder, Sweekaar Academy of Rehabilitation. Individual intervention and pre-post design was used for the study. Each group consisting 20 children diagnosed with autism between age group of 3 to 6 years. Group-I received intensive behavioral intervention 4 hours 6 days a week for 1year and Group-II received regular treatment for the same period. Pre-post assessment was done by VSMS, DST, GDT, and ISSA. Result indicates significant improvement in the domain of Social Relationship and Reciprocity (SRR), Emotional Responsiveness (ER), Speech Language and Communication (L&C), Behavior Patterns (BP), Cognitive Components (CC) Sensory Aspects (SA) and intellectual ability.

Transglutaminase (E.C. 2.3.2.13) are a family of enzymes that catalyze the covalent bond formation between open amine groups. They are widely used in food industries and their demand rises daily. Though they are available in mammalian tissues, fish and plants, the complex separation and purification process led to the search of Microbial Transglutaminase (MTGase). Finding a new microbial source of transglutaminase and the medium composition for MTGase production were the purpose of this work. Six Different types of Actinomycetes like strains were isolated from soil sample and two of them named PG03 and PG06 were selected based on their ability to produce 23 mg/ml and 21 mg/ml MTGase enzyme respectively. Strain PG03 was chosen for further studies and it was found to be a Streptomyces species. Standard enzyme production media composition is modified and tested to facilitate the optimized MTGase activity. Strategies like finest nitrogen and carbon source selection, revealing the key ingredients of media by full factorial design and their optimal concentration Box-Behnken design were adopted. At the 95% confidence level, second order polynomial model was applied to fit the research outcome. Under the proposed optimized conditions, the model predicted a transglutaminase yield of 21.7 mg/ml, very closely matching the experimental value of 24.1 mg/ml. The F-test was greater than the table value of 2.82 and the p-value of 0.004 clearly reveals that this regression was statistically significant at the 95% confidence level. Further, the proposed model has the ability to elucidate 48.8% response variation as indicated by the R2 of the regression value.