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1. DNA library preparation for Ion Torrent S5 sequencing

Author

Papathanassiou, Maria, Vlachostergios, Panagiotis J., Anagnostou, Maria, Thodou, Eleni, Tamposis, Ioannis, Mitrakas, Lampros, Zachos, Ioannis, Ioannou, Maria, Koukoulis, George K., Samara, Maria, and Tzortzis, Vassilios

Abstract

The current protocol desrcibes the steps followed for DNA library preparation for NGS use from fresh-frozen kidney tissue samples, including cancerous and normal tissues. Library amplification, purification and quantification is included.

Published
2023
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