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Aegla abrupta sp. nov. Mar��al and Teixeira (Figures 7���10) urn:lsid:zoobank.org:act: 8DC448C9-6106-447A-90E0-9B143E120783 Holotype Male [CLE 16.2 mm], Brazil, Paran��, Ortigueira, Paranapanema River basin, Tibagi River sub-basin, Formiga River, 24��11 �� 11.77���S, 50��56 �� 28.60���W, altitude 725 m, G.M. Teixeira, I.C. Mar��al and R.S. Vieira coll., 10 March 2020 (MZUEL 515). Paratypes 5 males [CLE 12.8���24.4 mm] and 8 females [CLE 12.5���18.2 mm], same data as holotype (MZUEL 499). Additional material examined 4 males [size not recorded] and 6 females [size not recorded], same data as holotype (MZUEL 504, genetic vouchers: Bold Systems access AEGLA 003-21, AEGLA 004-21). 1 male [CLE 16.7 mm], Brazil, Paran��, Ortigueira, Paranapanema River basin, Tibagi River sub-basin, tributary of the Formiga River, 24��10 �� 56.50���S, 50��56 �� 31.50���W, altitude 726 m, O.A. Shibatta coll., August 2014 (MZUEL 388). 1 male [CLE 20.1 mm] and 6 females [CLE 13.6���19.4 mm], Brazil, Paran��, Ortigueira, Paranapanema River basin, Tibagi River sub-basin, Piquira River, coordinates and coll. unknown, 2 February 2001 (MZUEL 440). 1 male [CLE 20.1 mm] and 1 female [CLE 19.2 mm], same locality, coordinates and coll. unknown, 25 April 2002 (MZUEL 441). Diagnosis Rostrum triangular, narrow base, extending beyond distal apex of compound eyes, carinate along entire length. Subrostral process well developed, occupying proximal half of subrostral margin, anterior and posterior margins forming acute angle. Orbital and extraorbital sinuses deep. Anterolateral spines reaching basal margin of cornea. Epigastric prominences pronounced. Protogastric lobes pronounced. Cervical groove trapezoidal. Areola subrectangular. Epibranchial area shortened. Lobe on proximal dorsal margin of dactylus rudimentary. Carpal ridge high on outer surface of carpus. Ventromesial border of cheliped ischium with 4 or 5 tubercles. Anteromesial region of third thoracic sternite abrupt. Anterior margin of second abdominal epimeron almost straight. Uropods wide. Description of holotype Carapace moderately convex, gastric region convex, dorsal surface scabrous, covered with punctations (Figure 7A). Rostrum triangular, narrow base (RBW/LMR = 0.79), extending beyond distal apex of compound eyes, carinate along entire length, small corneous scales on lateral margins and tip; ventral portion of rostrum much higher than dorsal in profile. Rostral carina beginning at level of protogastric lobes, with 2 rows of corneous scales extending next to apex. Subrostral process well developed, occupying proximal half of subrostral margin, triangular, tip rounded, anterior and posterior margins forming acute angle (88��) (Figure 8A). Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus U-shaped. Orbital spines well developed, with small terminal corneous scale. Anterolateral spines acuminate apically with small corneous scales terminally, reaching basal margin of cornea. Epigastric prominences pronounced, with corneous scales. Protogastric lobes pronounced, with corneous scales (Figure 8A). Gastric area elevated in relation to hepatic lobes and rostrum in lateral view. Demarcation between the first and the second hepatic lobes well defined, between the second and the third hepatic lobes weakly delimited. Lateral margins of hepatic lobes with small corneous scales and small setae. Cervical groove trapezoidal (Figure 8B). Transverse dorsal linea sinuous along its extension. Areola subrectangular (AH/[(APM+AAD)/2] = 2.20). Cardiac area trapezoidal (TDL/PMC = 1.39). Epibranchial area shortened, triangular, anterolateral angle with corneous scale, lateral margin with row of corneous scales and small setae (Figure 8B). Lateral margins of anterior and posterior branchial areas with row of corneous scales and small setae. Chelipeds unequal. Left cheliped largest (Figure 9 (A,B)). Dactylus: dorsal margin and outer surface with small corneous scales, inner surface with setal tufts and scales. Proximal dorsal margin with rudimentary lobe. Cutting margin with well-developed lobular basal tooth, followed by row of corneous scales to distal end. Row of small tufts of long setae next to cutting margin. Pre-dactylar lobe well developed, rounded, without corneous scales. Propodus: outer surface granular, globose aspect. Palmar crest subdisciform with outer surface excavated, margin poorly serrated, covered by acuminate corneous scales. Cutting margin of fixed finger with well-developed lobular basal tooth, followed by row of corneous scales to distal end. Inner and outer surfaces of fixed finger with rows of long setal tufts next to cutting margin. Carpus: dorsal margin with 2 proximal tubercles, median spine, distal spine longest, each tubercle or spine with small setae and terminal corneous scale, subterminal lobe well defined, pointed, with small corneous scales and setae. Inner surface with large tubercle, long setae next to dorsal margin. Outer surface with carpal ridge high, with small corneous scales. Merus: dorsolateral edge with distal tubercle, with corneous scale terminally, followed by row of tubercles decreasing in size proximally. Ventromesial edge with distal spine, with corneous scale terminally, followed by 4 tubercles of similar size, with corneous scale. Ventrolateral border with distal tubercle, with terminal corneous scale, followed by row of small tubercles. Ischium (Figure 7B): dorsolateral edge with spine, with corneous scale terminally. Ventromesial border with proximal tubercle, 2 median tubercles and large distal tubercle, each with terminal corneous scale. Ventrolateral border smooth. Minor cheliped (right) similar to major cheliped except as noted hereafter (Figure 9 (C, D)). Dactylus: cutting margin with rudimentary lobular basal tooth. Propodus: cutting margin with rudimentary lobular basal tooth. Ischium: ventromesial border with proximal tubercle, 3 small median tubercles and large distal tubercle, each with terminal corneous scale. Second, third and fourth pereiopods similar. Dactyli, propodi, carpi, meri and ischii with several rows of setal tufts and small scales on surface. Carpi and meri with row of small tubercles with terminal corneous scale along dorsal margin. Meri with row of small tubercles with terminal corneous scale along ventral margin. Meri and ischii with long setae concentrated along dorsal margin. Anteromesial region of third thoracic sternite abrupt, projecting between coxae of third maxillipeds, with scattered setae (Figure 7C). Fourth thoracic sternite with anterolateral angles produced anteriorly, with scattered setae (Figure 10A). Pleopods 2���5 absent. Anterolateral angle of second abdominal epimeron well defined, unarmed (Figure 10B). Ventral angle of third abdominal epimeron well defined with small corneous scales apically. Ventral angle of fourth abdominal epimeron well defined, unarmed. Anterior margin of second abdominal epimeron almost straight (Figures 7D, 10B). Uropods well developed, wide (WU/HWT = 1.04). Telson divided by longitudinal suture. Anterolateral and posterolateral margins well differentiated. Variations The areola can be subrectangular (n = 10), trapezoidal (n = 2) or rectangular (n = 1). The cardiac area varies from subrectangular (n = 9) to trapezoidal (n = 4). The palmar crest may appear rectangular instead of subdisciform. The anteromesial region of third thoracic sternite can be abrupt (n = 8) or tapered (n = 5). The anterolateral angle of second abdominal epimeron may present apical corneous scales instead of being naked as in the holotype. In most specimens, the ventral angle of the third abdominal epimeron is devoid of corneous scales. The uropods are narrow in a few specimens (n = 2). All measurements are summarised in Table 1. Etymology The specific epithet ���abrupta���, from the Latin abruptus, refers to the abrupt third thoracic sternite of the species. Distribution The new species has been collected in three watercourses within the Tibagi River subbasin, Paranapanema River basin. These watercourses are located in the city of Ortigueira, Paran�� state, Brazil. Conservation status We suggest that Aegla abrupta sp. nov. be assigned as ���Vulnerable��� (VU) under criteria B1, B2ab(iii) as defined by the IUCN (2019). This species has been recorded in three localities (B2a) and the known extent of occurrence is estimated to be less than 500 km 2 (B1). The streams where the specimens of Aegla abrupta sp. nov. were found are in agricultural and urbanised areas (B2b(iii)). Remarks Aegla abrupta sp. nov. can be separated from its congeners, mainly by, among other characters, subrostral process well developed (undeveloped in A. strinatii), occupying proximal half of subrostral margin (on proximal third of subrostral margin in A. lata, A. jacutinga, Aegla santosi sp. nov. and Aegla nanopedis sp. nov., and occupying median portion of subrostral margin in A. jaragua); anterior and posterior margins of subrostral process forming acute angle (right angle in A. lata and A. jacutinga, and obtuse angle in A. jaragua, Aegla santosi sp. nov. and Aegla nanopedis sp. nov.); orbital and extra-orbital sinuses deep (shallow in A. odebrechtii); epigastric prominences pronounced (poorly pronounced in A. lata and A. jacutinga); protogastric lobes pronounced (poorly pronounced in A. odebrechtii, A. lata, A. jarai and A. jaragua); cervical groove trapezoidal (U-shaped in A. lata and Aegla nanopedis sp. nov.); subterminal lobe of carpus well defined, pointed (poorly defined in A. strinatii); outer surface of carpus with carpal ridge high (low in A. jacutinga); dorsal margin of carpus and merus of second pereiopods with small tubercles with terminal corneous scale (with distal spine followed by tubercles decreasing in size proximally in A. jarai); ventral margin of merus of second pereiopods with small tubercles with terminal corneous scale (one or two small spines in A. castro and A. schmitti); anteromesial region of third thoracic sternite abrupt (tapered or truncate in A. lata, truncate in A. jaragua, and tapered in A. jacutinga, Aegla santosi sp. nov. and Aegla nanopedis sp. nov.); anterior margin of second abdominal epimeron almost straight (concave in A. castro and A. schmitti and slightly concave in A. jacutinga); uropods wide (narrow in A. lata and A. jaragua). Our Bayesian tree based on the COI mitochondrial gene (Figure 6) recovered Aegla abrupta sp. nov. as the sister species to Aegla nanopedis sp. nov., and both clustered in the same subclade as A. jacutinga. The interspecific genetic distances (Table 2) reinforce the relationships evidenced in the Bayesian analysis., Published as part of Mar��al, Ingrid Costa, P��ez, Fernanda Polli, Silva, Priscila Frazato Da, Souza-Shibatta, Lenice & Teixeira, Gustavo Monteiro, 2021, Cryptic diversity among populations of Aegla Leach, 1820 (Decapoda: Anomura: Aeglidae) from Tibagi River basin, Paran�� state, Brazil, with descriptions of three new species, pp. 2145-2171 in Journal of Natural History 55 (33 - 34) on pages 2155-2161, DOI: 10.1080/00222933.2021.1984599, http://zenodo.org/record/5727887, {"references":["IUCN. 2019. Guidelines for using the IUCN red list categories and criteria. Version 14. Prepared by the Standards and Petitions Subcommittee. [accessed 2020 Dec 30]. http: // www. iucnredlist. org / documents / RedListGuidelines. pdf."]}

Aegla s antosi sp. nov. Mar��al and Teixeira (Figures 11���14) urn:lsid:zoobank.org:act: 5751F246-51C1-4BC3-BBD5-CE21CB09CEC5 Holotype Male [CLE 19.3 mm], Brazil, Paran��, Tel��maco Borba, Paranapanema River basin, Tibagi River sub-basin, Codorna Branca stream, 24��15 �� 06.70���S, 50��31 �� 46.58���W, altitude 792 m, G. M. Teixeira, I.C. Mar��al and P.F. da Silva coll., 11 March 2020 (MZUEL 511). Paratypes 5 males [CLE 13.4���20.9 mm] and 5 females [CLE 14.1 ���16.0 mm], same data as holotype (MZUEL 500). 1 male [CLE 17.0 mm] and 1 female [CLE 16.9 mm], same locality, J.F.M. da Silva, E.S. da Silva and S.T. Bennemann coll., 7 August 2014 (MZUEL 470). Additional material examined 7 males [size not recorded] and 3 females [size not recorded], same data as holotype (MZUEL 505, genetic vouchers: Bold Systems access AEGLA 005-21, AEGLA 006-21). 1 male [CLE 13.3 mm], Brazil, Paran��, Tel��maco Borba, Paranapanema River basin, Tibagi River sub-basin, Varanal stream, 24��19 �� 59.70���S, 50��33 �� 37.20���W, altitude 761 m, S.T. Bennemann coll., 19 May 2005 (MZUEL 183). 3 males [CLE 12.7���16.8 mm], same locality and coll., March 2004 (MZUEL 349). 1 male [CLE 20.5 mm], same locality and coll., 24��20 �� 12.70���S, 50��32 �� 03.20���W, altitude 802 m, 11 February 2005 (MZUEL 332). 1 male [CLE 16.4 mm], same locality and coll., 24��20 �� 13.40���S, 50��35 �� 23.10���W, altitude 687 m, 9 November 2005 (MZUEL 344). 1 female [CLE 17.0 mm], Brazil, Paran��, Tel��maco Borba, Paranapanema River basin, Tibagi River sub-basin, Harmonia stream, coordinates and coll. unknown, 20 June 2006 (MZUEL 414). 4 females [CLE 12.5���15.8 mm], same locality, coordinates and coll. unknown, 16 March 2012 (MZUEL 415). 1 male [CLE 15.2 mm] and 1 female [CLE 15.4 mm], Brazil, Paran��, Tel��maco Borba, Paranapanema River basin, Tibagi River sub-basin, Moinho Velho stream, 24��13 �� 18.34���S, 50�� 37 �� 42.42���W, altitude 739 m, S.T. Bennemann coll., 7 August 2014 (MZUEL 426). 1 male [CLE 25.3 mm], same locality, J.F.M. da Silva coll., 3 November 2014 (MZUEL 427). 2 females [CLE 14.4 ���15.0 mm], Brazil, Paran��, Tel��maco Borba, Paranapanema River basin, Tibagi River subbasin, Col��nia stream, 24��10 �� 27.79���S, 50��37 �� 28.45���W, altitude 736 m, S.T. Bennemann coll., 7 August 2014 (MZUEL 437). Diagnosis Rostrum triangular, narrow base, extending beyond distal apex of compound eyes, carinate along entire length. Subrostral process well developed, occupying proximal third of subrostral margin, anterior and posterior margins forming obtuse angle. Orbital and extra-orbital sinuses deep. Anterolateral spines reaching basal margin of cornea. Epigastric prominences pronounced. Protogastric lobes pronounced. Areola trapezoidal. Cardiac area subrectangular. Epibranchial area slightly elongated. Lobe on proximal dorsal margin of dactylus rudimentary or absent. Carpal ridge high on outer surface of carpus. Ventromesial border of cheliped ischium with 4 or 5 tubercles. Ventral angles of third and fourth abdominal epimeron with corneous scales apically. Uropods wide., Published as part of Mar��al, Ingrid Costa, P��ez, Fernanda Polli, Silva, Priscila Frazato Da, Souza-Shibatta, Lenice & Teixeira, Gustavo Monteiro, 2021, Cryptic diversity among populations of Aegla Leach, 1820 (Decapoda: Anomura: Aeglidae) from Tibagi River basin, Paran�� state, Brazil, with descriptions of three new species, pp. 2145-2171 in Journal of Natural History 55 (33 - 34) on pages 2161-2163, DOI: 10.1080/00222933.2021.1984599, http://zenodo.org/record/5727887

Aegla buenoi Marçal & Teixeira n. sp. (Figs 3–5) urn:lsid:zoobank.org:act: F032E974-6EDE-4BA5-8577-17207631A62 Type-material. Holotype: male, Brazil, Paraná, Ibaiti, Paranapanema River basin, Cinzas River sub-basin, Carneiro stream, 23°57′19.71″S, 50°14′16.09″W, altitude 656 m, C.A. da Silva, R.H.C. do Nascimento and R. Rockembacher coll., 17.v.2016 (MZUEL 249). Paratypes: 1 male and 2 females, same data as holotype (MZUEL 245). 3 males and 2 females, Brazil, Paraná, Ibaiti, Paranapanema River basin, Cinzas River sub-basin, tributary of the Carneiro stream, 23°57′27.00″S, 50°14′5.00″W, altitude 620 m, G.M. Teixeira and J.J.S. Rosa coll., 12.vi.2017 (MZUEL 246, genetic vouchers: BOLD access AEGPR016-18, AEGPR017-18, AEGPR018-18, AEGPR019-18, AEGPR020-18). Type-locality. Carneiro stream, city of Ibaiti, Paraná state, Brazil. Geographical distribution. The new species has been collected in two streams within the Cinzas River subbasin, Paranapanema River basin. Both streams are located in the city of Ibaiti, Paraná state, Brazil. Diagnosis. Rostrum triangular, narrow base, reaching distal apex of compound eyes, carinate. Subrostral process well developed, occupying proximal half of subrostral margin, anterior and posterior margins forming obtuse angle (122°). Extra-orbital sinus deep. Anterolateral spines not reaching basal margin of cornea. Epigastric prominences pronounced and oval. Protogastric lobes pronounced. Cervical groove U-shaped. Cardiac area subrectangular. Proximal dorsal margin of dactylus with rudimentary or absent lobe. Palmar crest disciform, outer surface excavated. Subterminal lobe of carpus well defined, pointed. Ventromesial border of cheliped ischium with 4 or 5 tubercles. Anteromesial region of third thoracic sternite tapered. Anterior angle of second abdominal epimeron and ventral angles of third and fourth abdominal epimeron with corneous scale apically. Anterior margin of second abdominal epimeron almost straight. Uropods wide. Description of holotype. Carapace moderately convex, gastric region convex, dorsal surface scabrous, covered with punctations, anterior and posterior branchial areas expanded laterally (CW/CLE = 1.02) (Figs 3A, 4A). Rostrum triangular, narrow base (RBW/LMR = 0.98), reaching distal apex of compound eyes, carinate along entire length, small corneous scales on lateral margins, tip apparently broken; ventral portion of rostrum much higher than dorsal in profile. Rostral carina beginning at level of protogastric lobes, with row of corneous scales extending next to apex. Subrostral process well developed and covered by small setae, occupying proximal half of subrostral margin, tip rounded, anterior and posterior margins forming obtuse angle (122°) (Figs 3B, 4B). Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus U-shaped. Orbital spines well developed, with small terminal corneous scale. Anterolateral spines acuminate apically with small corneous scales terminally, not reaching basal margin of cornea. Epigastric prominences pronounced and oval, with corneous scales. Protogastric lobes pronounced, with corneous scales (Fig. 4B). Gastric area elevated in relation to hepatic lobes and rostrum in lateral view. Demarcation between hepatic lobes well defined. Lateral margins of hepatic lobes with small corneous scales. Cervical groove U-shaped. Transverse dorsal linea sinuous along its extension. Areola rectangular (AH/ [(APM+AAD)/2] = 2.54). Cardiac area subrectangular (TDL/PMC = 1.22). Epibranchial area strongly elongated, anterolateral angle with 2 corneous scales, lateral margin with row of small corneous scales and small setae. Lateral margins of anterior and posterior branchial areas with row of corneous scales and small setae. Chelipeds unequal, left largest. Major cheliped (left). Dactylus (Fig. 4C): dorsal margin and outer surface with small corneous scales, inner surface with setal tufts and scales; dorsal margin without proximal lobe; cutting margin with well-developed lobular basal tooth, followed by row of corneous scales up to distal end; row of small tufts of long setae next to cutting margin; pre-dactylar lobe well developed, rounded, smooth, without corneous scales. Propodus (Fig. 4C): outer surface granular, globose aspect; palmar crest disciform with outer surface excavated, margin poorly serrated, covered by corneous scales; fixed finger cutting margin with well-developed lobular basal tooth, followed by row of corneous scales up to distal end; inner and outer surfaces of fixed finger with rows of long setae tufts next to cutting margin. Carpus (Fig. 4C): dorsal margin with 2 proximal tubercles, 2 median spines, distal tubercle, double-tipped, each tubercle or spine with terminal corneous scale, subterminal lobe well defined, pointed, with small corneous scales, setae apically; inner surface with 2 distinct tubercles with acute corneous scale terminally; outer surface with carpal ridge high, with small corneous scales. Merus (Fig. 4D): dorsolateral edge with distal tubercle, with corneous scale terminally, followed by row of tubercles decreasing in size proximally; ventromesial edge with 2 distal spines, with corneous scale terminally, followed by 3 tubercles of similar size, with corneous scale; ventrolateral border with 2 distal tubercles, with terminal corneous scale, followed by several small tubercles proximally. Ischium (Figs 3C, 4D): dorsolateral edge with spine, with corneous scale terminally; ventromesial border with large proximal tubercle, 3 small median tubercles and large distal tubercle, each with terminal corneous scale; ventrolateral border smooth. Minor cheliped (right) similar to major cheliped except as noted hereafter. Dactylus (Fig. 4E): cutting margin with rudimentary lobular basal tooth. Propodus (Fig. 4E): cutting margin with rudimentary lobular basal tooth. Carpus (Fig. 4E): dorsal margin with 2 proximal tubercles, 2 median spines, distal spine, each with terminal corneous scale; inner surface with 5 tubercles, corneous scale terminally. Merus (Fig. 4F): ventromesial edge with distal spine, followed by 5 tubercles, each with terminal corneous scale. Ischium (Fig. 4F): ventromesial border with proximal tubercle, distal tubercle, each with terminal corneous scale. Second, third, and fourth pereiopods similar. Dactyli, propodi, carpi, meri and ischii with several rows of setal tufts and small scales on surface. Carpi and meri with row of tubercles with terminal corneous scale along dorsal margin. Meri and ischii with long setae concentrated along dorsal margin. Anteromesial region of third thoracic sternite tapered, projecting between coxae of third maxillipeds, with scattered setae. Fourth thoracic sternite with anterolateral angles produced anteriorly, with scattered setae (Fig. 3D). Anterolateral angle of second abdominal epimeron and ventral angles of third, fourth abdominal epimeron well defined, with corneous scale apically. Anterior margin of second abdominal epimeron almost straight (Fig. 3E). Uropods well developed, wide (WU/HWT = 1.04). Pleopods 2–5 absent. Telson divided by longitudinal suture. Anterolateral and posterolateral margins well differentiated. Variations. The rostrum is generally narrow base, but in some paratypes the base is wider than usual (RBW/ LMR = 1.03 ± 0.04; n = 5). In some specimens, the anterolateral spines can reach the basal margin of the cornea. The areola can be subrectangular (AH/[(APM+AAD)/2] = 2.12 ± 0.20; n = 4), trapezoidal (APM/AAD = 1.79 ± 0.10; n = 3), or rectangular (AH/[(APM+AAD)/2] = 2.31; n = 1). The cardiac area varies from subrectangular (TDL/PMC = 1.28 ± 0.06; n = 7) to trapezoidal (TDL/PMC = 1.45; n = 1). The proximal lobe on the dorsal margin of the dactylus of the major and minor chela may be rudimentary (as opposed to absent) (Fig. 5A). The palmar crest is rectangular instead of disciform in small individuals (CLE Biology. Unknown. Etymology. The specific epithet is given in honor of Dr. Sérgio Luiz de Siqueira Bueno, for his outstanding contributions to the taxonomy and knowledge of the biology of aeglids. Molecular data. Sequences of COI generated in this study were deposited in the BOLD database (access numbers AEGPR016-18, AEGPR017-18, AEGPR018-18, AEGPR019-18, AEGPR020-18).Altogether we analyzed 884 base pairs (bp) of COI with no insertions, deletions, or stop-codon. The mean base frequencies were: A = 0.2880, C = 0.1444, G = 0.1537, T = 0.4140. The genetic distance between A. buenoi n. sp. and its congeners ranged from 2.1% to 4.8% (Table 2). Aegla jacutinga showed the lowest divergence relative to A. buenoi n. sp., and the other species. In contrast, A. strinatii was found to be most distantly related to the new species and exhibited higher interspecific variations than the others, with a minimum K2P divergence of 3.8%. Most of the species showed intraspecific distances lower than 0.4%. Aegla castro and A. schmitti, however, presented intraspecific variations higher than 2.6% (Table 2). Regarding species delimitation, the GMYC analysis indicated the presence of 10 independent strains (Fig. 6). Two distinct clusters, with various well-supported subclusters, were obtained. Interestingly, the sequences of A. castro and A. schmitti formed non-monophyletic groups. On the other hand, all sequences of A. buenoi n. sp. were joined in the same cluster, indicating the presence of a single species., Published as part of Marçal, Ingrid Costa, Páez, Fernanda Polli, Souza-Shibatta, Lenice, Sofia, Silvia Helena & Teixeira, Gustavo Monteiro, 2021, Aegla buenoi n. sp. (Decapoda: Anomura): first record of aeglid crab from Cinzas River basin, Brazil, pp. 291-303 in Zootaxa 5005 (3) on pages 294-299, DOI: 10.11646/zootaxa.5005.3.4, http://zenodo.org/record/5141770

Este trabalho apresenta resultados parciais de uma pesquisa desenvolvida para compreender a dinâmica de transposição para a sala de aula de uma metodologia de ensino de ciências baseada na construção de brinquedos científicos. Essa metodologia foi aplicada em dois cursos de capacitação para professores de ciências do ensino fundamental. Considerando o saber formativo como aquele desenvolvido nos cursos de capacitação e o saber experiencial como aquele incorporado pelo professor em seu trabalho cotidiano, nosso objetivo foi compreender a dinâmica de transposição do saber formativo para o saber experiencial. Para isso, conduzimos um estudo qualitativo norteado pela observação participante, tendo como fontes de dados: registros em notas de campo, entrevistas semiestruturadas, questionários e registros em vídeo e fotográfico das ações desenvolvidas nos cursos e de seus desdobramentos em sala de aula. A análise dos dados foi conduzida numa perspectiva etnometodológica crítico-reflexiva, norteada pelos olhares dos pesquisadores-formadores e dos professores. Os resultados sugerem que o reconhecimento e a valorização do saber experiencial do professor favorecem a interlocução com o saber formativo, reduzindo a distância entre as intenções formativas da capacitação e a prática docente em sala de aula.

Aegla okora Páez & Teixeira n. sp. (Figs 2, 3, 4, 5, 6,) Type-material. Holotype: Male (CLE 21.3 mm), Brazil, Paraná, Pinhão, Iguaçu River basin, Tapera River, 25°41’39.51”S, 51°40’13.23”W, G.M. Teixeira, F.P. Paez and R.A. Gregati coll., February 2018 (MZUEL 250). Paratypes: 10 males (CLE 10.4 ¯23.0 mm) and 10 females (CLE 13.0¯18.0 mm), same data as holotype (MZUEL 251, genetic voucher: Bold Systems access AEGBR001-18, AEGBR002-18, AEGBR003-18, AEGBR004-18). Type-locality. Tapera River, city of Pinhão, Paraná, Brazil. Geographical distribution. Known only from the type-locality, despite searching in five nearby streams within a radius of the 32 km. Diagnosis. Triangular rostrum with narrow base. Subrostral process developed, anteriorly oriented at a 45° with the rostrum. Epigastric prominences and protogastric lobes pronounced, with scales and small setae. Anterolateral spine reaching basal margin of cornea. Branchial region swollen. Areola trapezoidal. Cardiac area trapezoidal. Proximal dorsal margin of movable finger of cheliped without lobe. Palmar crest of major cheliped rectangular. Anterolateral angle of second abdominal epimeron unarmed, with setae. Ventromesial border of ischium of the cheliped ornate with three tubercles, one proximal, one median and one distal. Uropodal (endopods) wide. Description of male holotype. Carapace convex, branchial region swollen. Rostrum triangular, base narrow (RBW/LMR = 0.87), extending beyond distal apex of compound eyes, with scales on lateral margins. Rostral carina beginning at level of protogastric lobes, with two parallel rows of scales becoming one row on distal third near apex (Fig. 2A, 3, 4A). Subrostral process developed forming angle of 45° (Fig. 4B). Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus with scales. Orbital spines developed, rounded. Anterolateral spines with corneous scales on lateral margin of carapace, reaching basal margin of cornea (Fig. 4A). Epigastric prominences pronounced, with scales and short setae. Protogastric lobes pronounced, with scales. Gastric area prominently inflated in relation to hepatic lobe and rostrum in lateral view. Demarcation between hepatic lobes well defined. Lateral margins of first hepatic lobe with corneous scale, second and third hepatic lobes with sparse scales. Cervical groove U-shaped (Fig. 4C). Transverse dorsal linea slightly sinuous throughout its extension, sinuosity more pronounced in mesial section. Areola trapezoidal (APM/AAD = 2.5). Cardiac area trapezoidal (TDL/PMC = 1.53) (Fig. 4C). Epibranchial area with spine or well developed scales on apex. Lateral margin of anterior branchial area with distal spine, setae and scales, posterior area with setae and scales. Anteromesial region of third thoracic sternite abrupt, with scattered setae on surface. Fourth thoracic sternite elevated in median region with setae, anterolateral angles well developed with spines (Fig. 2B, 4D). Chelipeds unequal in size (Figs 2A, 3). Major cheliped (left) (Fig. 5A). Dactylus: dorsal margin and outer surface granulate and ornamented with short scales. Pre-dactylar lobe absent. Proximal lobe on dorsal margin absent. Cutting margin with lobular basal tooth well developed proximally, with flattened corneous scales, followed by row of wide corneous scales up to distal end. Propodus: outer surface granulate. Palmar crest rectangular with outer surface excavated, margin with scales. Cutting margin of fixed finger with flattened corneous scales over its entire surface, with lobular basal tooth well developed proximally and acuminate corneous scale on distal end. Scattered tufts of long setae over inner surface, and alongside inner and outer surfaces next to cutting margin. Scattered scales and scales clustered into groups of 2 or 3 on inner surface. Carpus: dorsal margin with two tubercles proximally, two median spines with terminal corneous scale, one tubercle, internally displaced from the margin, distally, with terminal corneous scale, and sub-terminal lobe well defined, pointed, with corneous scales and setae apically. Inner surface with three tubercles with terminal corneous scale and setae. Outer surface with carpal ridge elevated along entire length, with scales clustered into groups of 3 – 5. Merus: dorsal margin with one tubercle. Dorsolateral edge with row of corneous scales and tubercles with corneous scales on distal third. Ventromesial edge with five tubercles decreasing in size proximally. Ventrolateral border with two tubercles distally, followed by row of scales clustered into groups of 2 – 3. Ischium: dorsolateral edge with distal spine with terminal corneous scale. Ventromesial border ornamented with one proximal tubercle, one median tubercle and one distal tubercle with one terminal corneous scale each (Fig. 2C, 6A). Minor chelipeds (right) similar to major chelipeds except as noted hereafter (Fig. 2D, 5B, 6B): Propodus: palmar crest rectangular to subdisciform. Merus: ventromesial edge with four tubercles decreasing in size proximally. Second, third and fourth pereiopods morphologically similar; general surface of dactylus, propodus and carpus with longitudinal lines of short setae and scales; dorsal margin of merus and ischium with long tufts setae; ventral margin of ischium with tufts of setae. Pleopods 2–5 absent. Anterolateral angle of second abdominal epimeron unarmed, with small setae (Fig. 2E, 6C). Anterior margin of second abdominal epimeron slightly concave. Uropods wide (WU/HWT = 1.18). Telson divided by longitudinal suture (Fig. 6D). Variations. Anterolateral angle of the carapace projected with a conical spine, protruding anteriorly, may just reach basal margin of the cornea. Of the 20 paratypes analyzed, the spine in 13 individuals is longer, extending beyond the basal margin of the cornea. The shape of the cardiac area may vary in some specimens, being trapezoidal (n = 15) or subrectangular (n = 5). The third thoracic sternite may vary from abrupt (n = 13) to tapered (n = 7). Uropods may vary between narrow (n = 9) and wide (n = 11). Biology. Unknown. Etymology. The specific epithet “okora”, from the indigenous Kaingang language “ȍkor” means “pine cone seed in the water”, refers Pinhão City where the type-locality is located. It is a noun in apposition. Molecular data. A total of 511 bp of COI were analyzed. No insertions, deletions or stop-codons were detected, indicating that all amplified regions correspond to a functional portion of the COI gene. The genetic distance between Aegla okora n. sp. and the other species included in the analysis ranged from 0.017 to 0.041 (Tab. 2). Aegla parana e A. schmitti presented the smallest genetic distance from A. okora n. sp., with a value of 0.017 for both. On the other hand, A. meloi was most divergent from A. okora n. sp., with a value of 0.041. The intra-populational distance ranged from 0.000 in A. parva to 0.002 in A. okora n. sp. However, A. parana and A. schmitti showed an intraspecific variation of up to 0.044 and 0.024, respectively (Tab. 2). The GMYC analysis suggests the presence of seven independent strains within the analyzed samples. All sequences of Aegla okora n. sp. were grouped in a single clade, indicating a single species. On the other hand, the disjunct distribution of the sequences of two species analyzed (A. parana and A. schmitti) suggests merophyletic clusters (Fig. 7).

Decapoda Latreille, 1802 Anomura H. Milne Edwards, 1832, Published as part of P��ez, Fernanda Polli, Mar��al, Ingrid Costa, Souza-Shibatta, Lenice, Gregati, Rafael Augusto, Sofia, Silvia Helena & Teixeira, Gustavo Monteiro, 2018, A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Igua��u River basin, Brazil, pp. 335-346 in Zootaxa 4527 (3) on page 338, DOI: 10.11646/zootaxa.4527.3.3, http://zenodo.org/record/2612284

Aegla okora P��ez & Teixeira n. sp. (Figs 2, 3, 4, 5, 6,) Type-material. Holotype: Male (CLE 21.3 mm), Brazil, Paran��, Pinh��o, Igua��u River basin, Tapera River, 25��41���39.51���S, 51��40���13.23���W, G.M. Teixeira, F.P. Paez and R.A. Gregati coll., February 2018 (MZUEL 250). Paratypes: 10 males (CLE 10.4 ��23.0 mm) and 10 females (CLE 13.0��18.0 mm), same data as holotype (MZUEL 251, genetic voucher: Bold Systems access AEGBR001-18, AEGBR002-18, AEGBR003-18, AEGBR004-18). Type-locality. Tapera River, city of Pinh��o, Paran��, Brazil. Geographical distribution. Known only from the type-locality, despite searching in five nearby streams within a radius of the 32 km. Diagnosis. Triangular rostrum with narrow base. Subrostral process developed, anteriorly oriented at a 45�� with the rostrum. Epigastric prominences and protogastric lobes pronounced, with scales and small setae. Anterolateral spine reaching basal margin of cornea. Branchial region swollen. Areola trapezoidal. Cardiac area trapezoidal. Proximal dorsal margin of movable finger of cheliped without lobe. Palmar crest of major cheliped rectangular. Anterolateral angle of second abdominal epimeron unarmed, with setae. Ventromesial border of ischium of the cheliped ornate with three tubercles, one proximal, one median and one distal. Uropodal (endopods) wide. Description of male holotype. Carapace convex, branchial region swollen. Rostrum triangular, base narrow (RBW/LMR = 0.87), extending beyond distal apex of compound eyes, with scales on lateral margins. Rostral carina beginning at level of protogastric lobes, with two parallel rows of scales becoming one row on distal third near apex (Fig. 2A, 3, 4A). Subrostral process developed forming angle of 45�� (Fig. 4B). Eyestalk and cornea well developed. Orbital and extra-orbital sinuses deep. Orbital sinus with scales. Orbital spines developed, rounded. Anterolateral spines with corneous scales on lateral margin of carapace, reaching basal margin of cornea (Fig. 4A). Epigastric prominences pronounced, with scales and short setae. Protogastric lobes pronounced, with scales. Gastric area prominently inflated in relation to hepatic lobe and rostrum in lateral view. Demarcation between hepatic lobes well defined. Lateral margins of first hepatic lobe with corneous scale, second and third hepatic lobes with sparse scales. Cervical groove U-shaped (Fig. 4C). Transverse dorsal linea slightly sinuous throughout its extension, sinuosity more pronounced in mesial section. Areola trapezoidal (APM/AAD = 2.5). Cardiac area trapezoidal (TDL/PMC = 1.53) (Fig. 4C). Epibranchial area with spine or well developed scales on apex. Lateral margin of anterior branchial area with distal spine, setae and scales, posterior area with setae and scales. Anteromesial region of third thoracic sternite abrupt, with scattered setae on surface. Fourth thoracic sternite elevated in median region with setae, anterolateral angles well developed with spines (Fig. 2B, 4D). Chelipeds unequal in size (Figs 2A, 3). Major cheliped (left) (Fig. 5A). Dactylus: dorsal margin and outer surface granulate and ornamented with short scales. Pre-dactylar lobe absent. Proximal lobe on dorsal margin absent. Cutting margin with lobular basal tooth well developed proximally, with flattened corneous scales, followed by row of wide corneous scales up to distal end. Propodus: outer surface granulate. Palmar crest rectangular with outer surface excavated, margin with scales. Cutting margin of fixed finger with flattened corneous scales over its entire surface, with lobular basal tooth well developed proximally and acuminate corneous scale on distal end. Scattered tufts of long setae over inner surface, and alongside inner and outer surfaces next to cutting margin. Scattered scales and scales clustered into groups of 2 or 3 on inner surface. Carpus: dorsal margin with two tubercles proximally, two median spines with terminal corneous scale, one tubercle, internally displaced from the margin, distally, with terminal corneous scale, and sub-terminal lobe well defined, pointed, with corneous scales and setae apically. Inner surface with three tubercles with terminal corneous scale and setae. Outer surface with carpal ridge elevated along entire length, with scales clustered into groups of 3 ��� 5. Merus: dorsal margin with one tubercle. Dorsolateral edge with row of corneous scales and tubercles with corneous scales on distal third. Ventromesial edge with five tubercles decreasing in size proximally. Ventrolateral border with two tubercles distally, followed by row of scales clustered into groups of 2 ��� 3. Ischium: dorsolateral edge with distal spine with terminal corneous scale. Ventromesial border ornamented with one proximal tubercle, one median tubercle and one distal tubercle with one terminal corneous scale each (Fig. 2C, 6A). Minor chelipeds (right) similar to major chelipeds except as noted hereafter (Fig. 2D, 5B, 6B): Propodus: palmar crest rectangular to subdisciform. Merus: ventromesial edge with four tubercles decreasing in size proximally. Second, third and fourth pereiopods morphologically similar; general surface of dactylus, propodus and carpus with longitudinal lines of short setae and scales; dorsal margin of merus and ischium with long tufts setae; ventral margin of ischium with tufts of setae. Pleopods 2���5 absent. Anterolateral angle of second abdominal epimeron unarmed, with small setae (Fig. 2E, 6C). Anterior margin of second abdominal epimeron slightly concave. Uropods wide (WU/HWT = 1.18). Telson divided by longitudinal suture (Fig. 6D). Variations. Anterolateral angle of the carapace projected with a conical spine, protruding anteriorly, may just reach basal margin of the cornea. Of the 20 paratypes analyzed, the spine in 13 individuals is longer, extending beyond the basal margin of the cornea. The shape of the cardiac area may vary in some specimens, being trapezoidal (n = 15) or subrectangular (n = 5). The third thoracic sternite may vary from abrupt (n = 13) to tapered (n = 7). Uropods may vary between narrow (n = 9) and wide (n = 11). Biology. Unknown. Etymology. The specific epithet ���okora���, from the indigenous Kaingang language �����kor��� means ���pine cone seed in the water���, refers Pinh��o City where the type-locality is located. It is a noun in apposition. Molecular data. A total of 511 bp of COI were analyzed. No insertions, deletions or stop-codons were detected, indicating that all amplified regions correspond to a functional portion of the COI gene. The genetic distance between Aegla okora n. sp. and the other species included in the analysis ranged from 0.017 to 0.041 (Tab. 2). Aegla parana e A. schmitti presented the smallest genetic distance from A. okora n. sp., with a value of 0.017 for both. On the other hand, A. meloi was most divergent from A. okora n. sp., with a value of 0.041. The intra-populational distance ranged from 0.000 in A. parva to 0.002 in A. okora n. sp. However, A. parana and A. schmitti showed an intraspecific variation of up to 0.044 and 0.024, respectively (Tab. 2). The GMYC analysis suggests the presence of seven independent strains within the analyzed samples. All sequences of Aegla okora n. sp. were grouped in a single clade, indicating a single species. On the other hand, the disjunct distribution of the sequences of two species analyzed (A. parana and A. schmitti) suggests merophyletic clusters (Fig. 7)., Published as part of P��ez, Fernanda Polli, Mar��al, Ingrid Costa, Souza-Shibatta, Lenice, Gregati, Rafael Augusto, Sofia, Silvia Helena & Teixeira, Gustavo Monteiro, 2018, A new species of Aegla Leach, 1820 (Crustacea, Anomura) from the Igua��u River basin, Brazil, pp. 335-346 in Zootaxa 4527 (3) on pages 338-342, DOI: 10.11646/zootaxa.4527.3.3, http://zenodo.org/record/2612284

Stratiodrilus haswelli Harrison, 1928 Type-locality: Unknown, not provided in the original description. Host and locality records: Astacoides madagascariensis (Milne Edwards & Audouin) (locality not provided) (Harrison 1928). Distribution: Madagascar., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 418, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Harrison, L. (1928) On the genus Stratiodrilus (Archiannelida: Histriobdellidae), with a description of a new species from Madagascar. Records of the Australian Museum, 16, 116 - 122. https: // doi. org / 10.3853 / j. 0067 - 1975.16.1928.783"]}

Stratiodrilus vilae Amato, 2001 Type-locality: Municipality of Taquara, State of Rio Grande do Sul, Brazil. Host and locality records: Parastacus brasiliensis (von Martens) ���Tributary of the Mineiro creek, Taquara (RS) (Amato 2001); unidentified creek of Mariana Pimentel (RS) (Amato 2001). Parastacus defossus Faxon��� Swampy area in Mariana Pimentel (RS) (Amato 2001). Distribution: Brazil., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 419, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Amato, J. F. R. (2001) A new species of Stratiodrilus (Polychaeta, Histriobdellidae) from freshwater crayfishes of southern Brazil. Iheringia, Serie Zoologia, 90, 37 - 44. https: // doi. org / 10.1590 / S 0073 - 47212001000100004"]}

Stratiodrilus circensis Steiner & Amaral, 1999 Type-locality: Piraquara River, Municipality of Piraquara, State of Paraná, Brazil. Host and locality records: Aegla abtao Schmitt—Toltén River (CHI) (Steiner & Amaral 1999). Aegla bahamondei Jara—Toltén River (CHI) (Steiner & Amaral 1999). Aegla castro Schmitt—Couro stream, Tibagi River Basin (PR) (Present study). Aegla laevis (Latreille) —Riñihue Lake (CHI) (Steiner & Amaral 1999); Negro River and lakes in Parque Nacional Nahuel Huapí (ARG) (Steiner & Amaral 1999). Aegla lata Bond-Buckup & Buckup—Bule stream, a tributary of the Apertados River, Tibagi River Basin (PR) (Present study). Aegla leptodactyla Buckup & Rossi—Divisa River, a tributary of the Pelotas River, municipality of São José dos Ausentes (RS) (Daudt & Amato 2007). Aegla neuquensis neuquensis (Schmitt) — Chico River (TUC) (Steiner & Amaral 1999). Aegla parana Schmitt—Pinhão River, Iguaçu River Basin (PR) (Present study). Aegla schmitti Hobbs III—Carvalho River, a tributary of Piraquara River, Parque Nacional Guaricana (PR) (Daudt & Amato 2007). Aegla sp.—Riñihue Lake (CHI) (Steiner & Amaral 1999). Aegla sp.— Piraquara River (PR) (Steiner & Amaral 1999). Aegla sp.—Lageadão river, Ivaí river Basin (PR) (Present study). Unspecified species of Aeglidae and Parastacidae—from Petorca to Chiloé (CHI) (Steiner & Amaral 1999). Distribution: Argentina, Brazil, and Chile.

Stratiodrilus arreliai Amaral & Morgado, 1997 Type-locality: Peroba Cave, Municipality of S��o Pedro, State of S��o Paulo, Brazil. Host and locality records: Aegla perobae Hebling & Rodrigues���Peroba Cave, municipality of S��o Pedro (SP) (Amaral & Morgado 1997). Aegla sp.���Creeks near Jaragu�� Peak, municipality of S��o Paulo (SP) (Amaral & Morgado 1997). Distribution: Brazil., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 417, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Amaral, A. C. Z. & Morgado, E. H. (1997) Stratiodrilus (Annelida: Polychaeta: Histriobdellidae) associated with a freshwater decapod, with the description of a new species. Proceedings of the Biological Society of Washington, 110, 471 - 475."]}

Stratiodrilus platensis Cordero, 1927 Type-locality: Probably Sol��s Chico and Miguelete streams, Departamento de Canelones, Uruguay. Host and locality records: Aegla laevis (Latreille) ���Sol��s Chico and Miguelete streams (CNL) (Cordero 1927). Samastacus spinifrons (Philippi) ���Chile (locality not provided) (Rudolph 2002). Aegla sp.���Lakes of Parque Nacional Nahuel Huap�� (ARG) (Dioni 1972). Parastacus sp.���Lakes of Parque Nacional Nahuel Huap�� (ARG) (Dioni 1972). Trichodactylus sp.���Creeks near Canan��ia (SP), Southeast Brazil (Steiner & Amaral 1999). Distribution: Argentina, Brazil, Chile, and Uruguay., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 418, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Cordero, B. H. (1927) Un nuevo arquianelido, Stratiodrilus platensis sp. n. que habita sobre Aegla laevis laevis (Latreille). Physis, 7, 574 - 578.","Dioni, W. (1972) Didymorchis, Temnocephala (Platyhelmintha) y Stratiodrilus (Annelida) vermes epizoicos sobre Aegla y Parastacus (Crustacea: Decapoda) de Lagos Andino Patagonicos. Notas taxonomicas e biogeograficas. Acta Zoologica Lilloana, 29, 167 - 179."]}

Stratiodrilus pugnaxi Vila & Bahamonde, 1985 Type-locality: Reumén, Valdivia, Chile. Host and locality records: Parastacus pugnax (Poeppig) —Reumén (VAL) (Vila & Bahamonde 1985). Parastacus sp.—Andalién River, Chaimávida (CON) (Moyano et al. 1993). Unspecified species of Aeglidae and Parastacidae—from Petorca to Chiloé (CHI) (Steiner & Amaral 1999). Distribution: Chile.

Stratiodrilus robustus Steiner & Amaral, 1999 Type-locality: Ribeira Valley, State of São Paulo, Brazil. Host and locality records: Trichodactylus sp.—Streams near Ribeira Valley (SP) (Steiner & Amaral 1999). Distribution: Brazil.

Stratiodrilus tasmanicus Haswell, 1900 Type-locality: Hobart, Tasmania, Australia. Host and locality records: Astacopsis franklinii (Grey) ���Hobart (TAS) (Haswell 1900). Astacopsis franklinii var. tasmanicus Erickson���Hobart (TAS) (Haswell 1900). Distribution: Australia., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 418, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Haswell, W. A. (1900) On a new Histriobdellid. Quarterly Journal of Microscopical Science, 43, 299 - 335."]}

Stratiodrilus robustus Steiner & Amaral, 1999 Type-locality: Ribeira Valley, State of S��o Paulo, Brazil. Host and locality records: Trichodactylus sp.���Streams near Ribeira Valley (SP) (Steiner & Amaral 1999). Distribution: Brazil., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 418, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638

Stratiodrilus novaehollandiae Haswell, 1913 Type-locality: Probably Blue Mountains, New South Wales, Australia. Host and locality records: Astacopsis serratus (Shaw) ���Blue Mountains (NSW); Murrumbidgee River, Murray River System (NSW) (Haswell 1913). Cherax dispar Riek���Gap Creek, Mount Coottha (QLD) (Cannon & Jennings 1987). Cherax punctatus Clark���Gap Creek, Mount Coottha (QLD) (Cannon & Jennings 1987). Distribution: Australia., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 418, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Haswell, W. A. (1913) Notes on the Histriobdellidae. Quarterly Journal of Microscopical Science, 59, 197 - 230.","Cannon, L. R. G. & Jennings, J. B. (1987) Occurence and nutritional relationships of four ectosymbiotes of the freshwater crayfishes Cherax dispar Riek and Cherax punctatus Clark (Crustacea: Decapoda) in Queensland. Australian Journal of Marine and Freshwater Research, 38, 419 - 427. https: // doi. org / 10.1071 / MF 9870419"]}

Stratiodrilus pugnaxi Vila & Bahamonde, 1985 Type-locality: Reum��n, Valdivia, Chile. Host and locality records: Parastacus pugnax (Poeppig) ���Reum��n (VAL) (Vila & Bahamonde 1985). Parastacus sp.���Andali��n River, Chaim��vida (CON) (Moyano et al. 1993). Unspecified species of Aeglidae and Parastacidae���from Petorca to Chilo�� (CHI) (Steiner & Amaral 1999). Distribution: Chile., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 418, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638

Stratiodrilus aeglaphilus Vila & Bahamonde, 1985 Type-locality: Ma��po River, Talagante area, Central Chile, Chile. Host and locality records: Aegla laevis (Latreille) ���Ma��po River, Talagante area (CNC) (Vila & Bahamonde 1985); Aguas Claras stream (CNC) (Vila & Bahamonde 1985; Pardo et al. 2008). Aegla laevis laevis (Latreille) ��� Pe��aflor tributary, Ma��po River (CNC) (Vila & Bahamonde 1985). Aegla laevis talcahuano Schmitt������Estero��� Bellavista between Lirqu��n and Tom�� (CON) (Moyano et al. 1993). Unspecified species of Aeglidae and Parastacidae���from Petorca to Chilo�� (CHI) (Steiner & Amaral 1999). Distribution: Chile., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 417, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638

Stratiodrilus circensis Steiner & Amaral, 1999 Type-locality: Piraquara River, Municipality of Piraquara, State of Paran��, Brazil. Host and locality records: Aegla abtao Schmitt���Tolt��n River (CHI) (Steiner & Amaral 1999). Aegla bahamondei Jara���Tolt��n River (CHI) (Steiner & Amaral 1999). Aegla castro Schmitt���Couro stream, Tibagi River Basin (PR) (Present study). Aegla laevis (Latreille) ���Ri��ihue Lake (CHI) (Steiner & Amaral 1999); Negro River and lakes in Parque Nacional Nahuel Huap�� (ARG) (Steiner & Amaral 1999). Aegla lata Bond-Buckup & Buckup���Bule stream, a tributary of the Apertados River, Tibagi River Basin (PR) (Present study). Aegla leptodactyla Buckup & Rossi���Divisa River, a tributary of the Pelotas River, municipality of S��o Jos�� dos Ausentes (RS) (Daudt & Amato 2007). Aegla neuquensis neuquensis (Schmitt) ��� Chico River (TUC) (Steiner & Amaral 1999). Aegla parana Schmitt���Pinh��o River, Igua��u River Basin (PR) (Present study). Aegla schmitti Hobbs III���Carvalho River, a tributary of Piraquara River, Parque Nacional Guaricana (PR) (Daudt & Amato 2007). Aegla sp.���Ri��ihue Lake (CHI) (Steiner & Amaral 1999). Aegla sp.��� Piraquara River (PR) (Steiner & Amaral 1999). Aegla sp.���Lagead��o river, Iva�� river Basin (PR) (Present study). Unspecified species of Aeglidae and Parastacidae���from Petorca to Chilo�� (CHI) (Steiner & Amaral 1999). Distribution: Argentina, Brazil, and Chile., Published as part of Rosa, Jheimison Junior Da Silva, Mar��al, Ingrid Costa, Teixeira, Gustavo Monteiro & Aguiar, Aline, 2018, Checklist of species of Stratiodrilus Haswell, 1900 (Annelida: Histriobdellidae), and new host records from Southern Brazil, pp. 412-422 in Zootaxa 4399 (3) on page 417, DOI: 10.11646/zootaxa.4399.3.10, http://zenodo.org/record/1206638, {"references":["Daudt, L. C. C. & Amato, J. F. R. (2007) Morphological variation of Stratiodrilus circensis (Polychaeta, Histriobdellidae) from a new host, Aegla leptodactyla (Crustacea, Anomura, Aeglidae) with identification of its type host species. Zootaxa, 1450, 57 - 62."]}

Stratiodrilus arreliai Amaral & Morgado, 1997 Type-locality: Peroba Cave, Municipality of São Pedro, State of São Paulo, Brazil. Host and locality records: Aegla perobae Hebling & Rodrigues—Peroba Cave, municipality of São Pedro (SP) (Amaral & Morgado 1997). Aegla sp.—Creeks near Jaraguá Peak, municipality of São Paulo (SP) (Amaral & Morgado 1997). Distribution: Brazil.

As cianobactérias são organismos capazes de produzir uma diversidade de biocompostos de alto valor agregado, dentre eles, as ficobiliproteínas (FBP), pigmentos usados como corantes naturais para alimentos e produtos cosméticos, bem como marcadores fluorescentes moleculares. Este trabalho teve como objetivo o estudo do processo de produção e recuperação de FBP das cianobactérias, Nostoc sp. e Anabaena variabilis. Das FBPs, apenas ficocianina (FC), foi detectada no extrato de ambas as cepas. O meio de cultivo das cianobactérias foi modificado para avaliar o efeito da concentração de fonte de carbono (glicose) e da fonte de nitrogênio (nitrato de sódio) e do efeito da cor (branca, amarela, azul, verde e vermelha) sobre o crescimento de biomassa e de síntese da FC. Para a etapa de recuperação da FC, foi escolhido o sistema aquoso bifásico (SAB), técnica atrativa para biomoléculas, por apresentar grande quantidade de água nas duas fases, proporcionando um ambiente não tóxico e compatível. Neste sentido, foram avaliados o efeito da adição do NaCl ao SAB do tipo PEG-fosfato de potássio, na recuperação da FC. Além disso, investigou-se também o SAB empregando copolímeros tri-bloco de diferentes cadeias de PEO (poli (óxido de etileno)) (F68, L62 e L64) e o tipo de sal (citrato de sódio e fosfato de potássio) em diferentes proporções, para testar o aumento da recuperação e pureza inicial da FC. Os copolímeros são termossenssíveis, podendo ser recuperados e reutilizados por aquecimento, diferente do PEG. Assim, foi avaliada também a recuperação por termosseparação dos copolímeros utilizados. Na avaliação do efeito de parâmetros de cultivo, o melhor resultado foi encontrado na suplementação ao meio de cultivo para a cepa de Nostoc sp. de 2 g.L-1 de glicose e 5 mM de nitrato de sódio de forma combinada, obtendo concentrações de FC de 133,03 mg.g-1 e concentração celular de 0,53 g.L-1. Com relação ao efeito da cor da luz, de forma geral, a luz branca foi a cor que mais favoreceu o crescimento celular e quando combinada ao ensaio com suplementação, apresentou cerca de 0,43 g.L-1 para ambas as cepas estudadas após os 14 dias de cultivo. Para a produção de FC, na condição de suplementação do meio, a luz vermelha apresentou melhor resposta para as duas cepas, equivalentes a 141,97 mg.g-1 para Nostoc sp. e 161,06 mg.g-1 para A. variabilis. O estudo de recuperação da FC por SAB, indicou o efeito positivo do “salting-out” com a adição de cloreto de sódio (NaCl), com 6% (m/m) sendo a melhor condição para Nostoc sp., atingindo pureza (P) de 1,13, recuperação (R) de 84,5% e coeficiente de partição (K) igual a 9,46. Já para A. variabilis, as melhores condições foram para adição de 6 e 8% (m/m), atingindo (P) de 1,16, (R) de 83,35% e (K) de 9,80. Para o SAB do tipo copolímero-sal, e proporção 25% copolímero e 8% sal, L64+fosfato de potássio foi o melhor sistema para a recuperação da FC dos extratos das duas cepas, sendo que ocorreu a partição da proteína na fase fundo, alcançando (R) equivalente a 83,40% para cepa de Nostoc sp. e 86,76 % para A. variabilis. Ao alterar a proporção para 15% copolímero e 12% sal, a FC migrou para a fase topo, sendo que o sistema F68+fosfato de potássio possibilitou melhor resultado para as duas cepas, alcançando (R) de 86,46% para Nostoc sp. e 86,83% para A. variabilis. Na etapa de recuperação do copolímero por termosseparação, foi possível recuperar o L62 (94,50%) e o L64 (96,54%). Para o copolímero F68, não houve separação de fases na faixa de temperatura testada. O estudo mostrou que maiores teores de ficocianina podem ser obtidos ao modificar o meio de cultura das cianobactérias, principalmente com a suplementação de fontes de carbono e nitrogênio, e que a recuperação deste pigmento é viável por SAB, tanto para meios constituídos de PEG como copolímeros. Cyanobacteria are organisms capable of producing a diversity of biocomposites with high added value, including phycobiliproteins (PBP), which are pigments used as natural dyes for food and cosmetic products, as well as molecular fluorescent markers. This work aimed to study the process of production and recovery of PBP from cyanobacteria, Nostoc sp. and Anabaena variabilis. The only PBP detected in the extract of both strains was phycocyanin (PC). The medium of cyanobacteria was modified to evaluate the effect of the concentration of carbon source (glucose) and nitrogen source (sodium nitrate) and the effect of color (white, yellow, blue, green and red) on biomass growth and PC synthesis. For the PC recovery stage, the aqueous two-phase system (ATPS) was chosen, as an attractive technique for biomolecules, as it presents a large amount of water in both phases, providing a non-toxic and compatible environment. In this sense, the effect of adding NaCl to ATPS of PEG-potassium phosphate on PC recovery was evaluated. Furthermore, it was also tested ATPS using tri-block copolymers of different chains of PEO (poly (ethylene oxide)) (F68, L62 and L64) and the type of salt (sodium citrate and potassium phosphate) in different proportions, to test the increase in PC recovery and initial purity. Copolymers are thermo-sensitive and can be recovered and reused by heating, different from PEG. Thus, recovery was also evaluated by terms of separation of the copolymers used. In respect to the effect of cultivation parameters, the best result was found in supplementing the medium for the Nostoc sp. of 2 g.L-1 of glucose and 5 mM of sodium nitrate in a combined form, obtaining PC concentrations of 133.03 mg.g-1 and cell concentration of 0.53 g.L-1. Regarding the effect of the color of light, in general, using white light allowed the highest cell growth and when combined with the supplementation assay, it presented about 0.43 gL-1 for both strains studied after 14 days of cultivation. For the production of PC, under supplementation of the medium, the red light showed better response for both strains, equivalent to 141.97 mg.g-1 for Nostoc sp. and 161.06 mg.g-1 for A. variabilis. The study of PC recovery by ATPS, indicated the positive effect of “salting-out” with the addition of sodium chloride (NaCl), with 6% (w/w) being the best condition for Nostoc sp., Reaching purity (P) of 1,13, recovery (R) of 84.5% and partition coefficient (K) equal to 9.46. As for A. variabilis, the best conditions were for adding 6 and 8% (w/w), reaching (P) 1.16, (R) 83.35% and (K) 9.80. For ATPS of the copolymer-salt type, and 25% copolymer and 8% salt ratio, L64 + potassium phosphate was the best system for the recovery of PC from the extracts of the two strains, with the protein partitioning occurring in the bottom phase, reaching (R) equivalent to 83.40% for Nostoc sp. and 86.76% for A. variabilis. In the second proportion, 15% copolymer and 12% salt, the PC migrated to the top phase, with the F68 + potassium phosphate system providing a better result for both strains, reaching (R) of 86.46% for Nostoc sp. and 86.83% for A. variabilis. In the step of recovering the copolymer by thermal separation, it was possible to recover L62 (94.50%) and L64 (96.54%). For the F68 copolymer, there was no phase separation in the tested temperature range. The study showed that higher levels of phycocyanin can be obtained by modifying the medium of cyanobacteria, especially with the supplementation of carbon and nitrogen sources, and that the recovery of this pigment is viable by ATPS, both for PEG and copolymer media. Dissertação (Mestrado) 2022-11-19

As cianobactérias, conhecidas como algas azuis e verdes, são um grupo de microrganismos autótrofos fotossintetizantes. Elas são capazes de produzir uma diversidade de compostos bioativos, podendo ser metabólitos, como compostos antibacterianos, anticoagulantes, anti-inflamatórios, antivirais, antitumorais e citotóxicos ou compostos pigmentados, como clorofila a, b e c, beta-caroteno e ficobiliproteínas (FBP). Dentre os compostos pigmentados destacam-se as FBP, que são um conjunto de proteínas pigmentadas, hidrossolúveis e fluorescentes, com aplicações na indústria de alimentos como corantes naturais e na indústria farmacêutica como marcadores fluorescentes em diagnósticos de doenças. A principal FBP encontrada nas cia-nobactérias é a ficocianina (FC). Seu processo de obtenção é baseado em três etapas, represen-tadas por cultivo, extração e recuperação/purificação. O presente trabalho foi um estudo que abordou as etapas de produção e recuperação de FBP de duas cepas de cianobactérias pouco exploradas na literatura, Anabaena variabilis e Nostoc sp. Foram investigados diferentes mé-todos de extração das FBP, método de recuperação por Sistemas Aquosos Bifásicos (SAB) e produção de FBP por suplementação de fonte de carbono orgânico e nitrogênio ao meio de cultivo basal, visando aumentar sua produtividade. Para purificação foi utilizado SAB, por ser uma técnica atrativa para biomoléculas, pelo fato de possuir água em alta concentração em ambas as fases, sendo assim um ambiente compatível e não tóxico. O SAB utilizado foi do tipo polietilenoglicol (PEG)-sal, variando o peso molecular do PEG (1500 e 4000) e o tipo de sal (fosfato de potássio, sulfato de amônio e citrato de sódio) para purificação, em diferentes proporções, visando aumentar a pureza inicial das FBP obtidas pelo método de extração. Den-tre os resultados obtidos para extração, destacam-se a presença de apenas FC em ambas cia-nobactérias, melhor solução extratora sendo o tampão fosfato pH 7,0 0,1M, o melhor método de extração sendo por congelamento e descongelamento em banho ultrassônico obtendo as maiores concentrações de FC (32,8 µg/mL e 34,6 µg/mL) e purezas (0,545 e 0,5938) para Nos-toc sp. e Anabaena variabilis respectivamente e a melhor razão sólido-líquido de 0,4 g de célu-la/L de solução extratora, obtendo 0,35 mg de FC. Para a recuperação, a melhor condição en-contrada foi para o SAB PEG 1500-fosfato de potássio, para proporção (10% PEG e 15,9% sal), obtendo recuperação de 79,10%, coeficiente de partição de FC de 6,36 e pureza final de 1,34 o extrato de Nostoc sp. e recuperação de 82,10%, coeficiente de partição de FC de 5,73 e pureza final de 1,12 para o extrato de Anabaena variabilis. Para a suplementação ao meio de cultivo o melhor resultado foi a suplementação de glicose 2 g/L e nitrato de sódio 5 mM, ob-tendo aumento na concentração de FC de até 50% em relação ao meio basal, com produtivi-dade de 48,73 µg/mL para Nostoc sp. em 14 dias e 18,57 µg/mL para Anabaena variabilis em 10 dias. Cyanobacteria also known as algae blue green, are a group of autotrophic photosynthetic mi-croorganisms. They produce a variety of bioactive compounds, and may be metabolites like antibacterial, anticoagulant, anti-inflammatory, antiviral, antitumor and cytotoxic compounds or pigmented compounds, such as chlorophyll a, b and c, beta-carotene and phycobiliproteins (PBP). Among the pigmented compounds are the PBP, which are a set of pigmented proteins, water-soluble and fluorescent, with applications in the food industry as natural dyes and in the pharmaceutical industry as fluorescent markers in disease diagnosis. The main FBP found in cyanobacteria is phycocyanin (PC). The procurement process is based on three stages, repre-sented by cultivation, extraction and recovery / purification. The present work was a study about the production and recovery of PBP of two strains of cyanobacteria little explored in the literature, Anabaena variabilis and Nostoc sp. Different extraction methods of PBP, re-covery for Aqueous Two-Phase System (ATPS) and production of PBP by supplementation with organic carbon and nitrogen sources in addition to the basal culture medium were inves-tigated in order to increase their productivity. The ATPS was used since it is an attractive technique for biomolecules, because it has water in high concentration in both phases, being thus a compatible and non-toxic for proteins. The ATPS used was of the polyethylene glycol (PEG)-salt type, varying the molecular weight of the PEG (1500 and 4000) and the salt type (potassium phosphate, ammonium sulfate and sodium citrate) for purification, in different proportions, in order to increase the initial purity of the FBP obtained by the extraction meth-od. Results were obtained for extraction, only FC was found in both cyanobacteria, the best extractive solution being phosphate buffer pH 7.0 0.1M, the best method of extraction by freezing and thawing in ultrasonic bath obtaining the highest concentrations of FC (32.8 μg/mL and 34.6 μg/mL) and purities (0.54 and 0.59) for Nostoc sp. and Anabaena variabilis, respectively, and the best solid-liquid ratio of 0.4 g of cell/L of extractive solution, yielding 0.354 mg FC. For recovery, the best condition was ATPS PEG 1500-phosphate, in proportion (10% PEG and 15.9% salt), recovering 79.10%, partition coefficient of 6.36 and final purity of 1.34 the extract of Nostoc sp. and recovery of 82.10%, partition coefficient of 5,73 and final purity of 1.12 for the Anabaena variabilis extract. The best result for supplementation to the culture medium was 2 g/L glucose and 5 mM sodium nitrate, increasing the FC concentra-tion by up to 50% compared to the basal medium, with a yield of 48.73 μg/mL. Nostoc sp. at 14 days and 18.57 μg/mL for Anabaena variabilis at 10 days. Dissertação (Mestrado)