1. A synthetically simple, click-generated cyclam-based zinc(ll) sensor
- Author
-
Tamanini, E, Katewa, A, Sedger, LM, Todd, MH, and Watkinson, M
- Subjects
Electron Transport ,Mice ,Zinc ,Light ,Heterocyclic Compounds ,Animals ,Inorganic & Nuclear Chemistry ,Triazoles ,Binding, Competitive ,Fluorescence ,Cell Line ,Carbonic Anhydrases ,Fluorescent Dyes - Abstract
A cyclam-based macrocyclic sensor has been prepared using synthetically simple "click" chemistry to link a fluorophore to the macrocyclic receptor. This sensor shows high selectivity for Zn(ll) over a range of other metals, providing a significant enhancement of fluorescence intensity over a wide pH range. As such, this is the first cyclam-based sensor demonstrated to be selective for Zn(ll) and is the first example of a triazole being used as a coordinating ligand on an azamacrocycle. The sensor can access biologically available zinc in mammalian cells, sensing the Zn(ll) flux that exists during apoptotic cell death. © 2009 American Chemical Society.
- Published
- 2009