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The hypothalamus plays a vital role in coordinating essential neuroendocrine, autonomic, and somatomotor responses for survival and reproduction. While previous studies have explored population-level projections of hypothalamic neurons, the specific innervation patterns of individual hypothalamic axons remain unclear. To understand the organization of hypothalamic axon projections, we conducted a comprehensive reconstruction of single-cell projectomes from 7,180 mouse hypothalamic neurons expressing specific neuropeptides. Our analysis identified 31 distinct subtypes based on projectome-defined characteristics, with many exhibiting long-range axon collateral projections to multiple brain regions. Notably, these subtypes selectively targeted specific subdomains within downstream areas, either unilaterally or bilaterally. Furthermore, we observed that individual peptidergic neuronal types encompassed multiple projectome-defined subtypes, explaining their diverse functional roles. Additionally, by examining intra-hypothalamic axon projections, we uncovered six modular subnetworks characterized by enriched intramodular connections and distinct preferences for downstream targets. This modular organization of the intra-hypothalamic network likely contributes to the coordinated organization of hypothalamic outputs. In summary, our comprehensive projectome analysis reveals the organizational principles governing hypothalamic axon projections, providing a framework for understanding the neural circuit mechanisms underlying the diverse and coordinated functions of the hypothalamus.

In this work, the effects of proton-induced trap evolution on the electrical performances of AlGaN/GaN metal–insulator–semiconductor (MIS) high-electron-mobility-transistors (HEMTs) have been investigated in detail. Contrary to observed proton-induced degradation in sheet electron density (ns), carrier mobility (μn), and sheet resistance (RSH), a significant improvement in gate leakage current (IG) and off-state drain–source breakdown voltage (BVDS) is found in irradiated devices. The low-frequency noise (LFN) results show that the density of traps in the AlGaN layer and AlGaN/GaN interface increases after irradiation, which leads to a degradation in ns, μn, and RSH and an improvement in BVDS due to enhanced electron trapping and depletion. Furthermore, the measurement results obtained from the frequency-dependent conductance technique show that irradiated devices exhibit decreased trap density (DT) at the insulator/semiconductor interface with hardly changed trap energy level (ET), which confirms the observed proton-induced decrease in IG. An increase in DT at the AlGaN/GaN interface is also found after irradiation, which is consistent with LFN results. These traps with deeper ET induce a serious Coulomb scattering effect. This work provides valuable information for the systematic understanding of the proton irradiation effect of AlGaN/GaN MIS-HEMTs.

Background Nutritional status and inflammation are closely associated with poor outcome in malignant tumors. However, the prognostic impact of postoperative in these variables on breast cancer (BC) remains inconclusive. We aimed to determine whether prognostic nutritional index (PNI), systemic immune‐inflammation index (SII), neutrophil–lymphocyte ratio (NLR) and platelet–lymphocyte ratio (PLR) affect two long-term outcomes among patients after curative resection of BC. Methods We retrospectively reviewed 508 patients with BC treated with curative surgery between February 5, 2013 and May 26, 2020. All patients were divided into 3 groups based on tertiles (T1-T3) of PNI, SII, NLR, and PLR. The effects of four indexes on disease-free survival (DFS) and overall survival (OS) have been evaluated using Cox proportional hazards models and Kaplan–Meier method. Results Compared with PNI-lowest cases, patients with highest PNI showed significantly longer DFS (multivariate adjusted hazard ratio [HR] = 0.37, 95% confident interval [CI] 0.19–0.70, P for trend = 0.002), whereas higher PLR seemed to be marginally associated with poorer DFS (P for trend = 0.086 and 0.074, respectively). Subgroup analyses indicate the potential modification effects of family history of BC and radiotherapy on the prognosis value of PNI to DFS in BC patients (P for interaction = 0.004 and 0.025, respectively). In addition, the levels of three inflammatory indices, namely SII, NLR, and PLR might be positively related with increased age at diagnosis (all P for trend Conclusions A high PNI was associated with better DFS, supporting its roles as prognostic parameters for patients with BC. The nutritional status and systemic immune may exert great effects on patient prognosis. Further studies are warrant to explore the prognosis value of PLR.

Alkene hydrocarbonation reactions have been developed to supplement traditional electrophile-nucleophile cross-coupling reactions. The branch-selective hydroalkylation method applied to a broad range of unactivated alkenes remains challenging. Herein, we report a NiH-catalysed proximal-selective hydroalkylation of unactivated alkenes to access β- or γ-branched alkyl carboxylic acids and β-, γ- or δ-branched alkyl amines. A broad range of alkyl iodides and bromides with different functional groups can be installed with excellent regiocontrol and availability for site-selective late-stage functionalization of biorelevant molecules. Under modified reaction conditions with NiCl2(PPh3)2 as the catalyst, migratory hydroalkylation takes place to provide β- (rather than γ-) branched products. The keys to success are the use of aminoquinoline and picolinamide as suitable directing groups and combined experimental and computational studies of ligand effects on the regioselectivity and detailed reaction mechanisms.

During the process of synaptic formation, neurons require not only certain principles for selecting partners to form synapses but also additional mechanisms to avoid undesired cells. However, the means to get around unwanted partners remains largely unknown. In this study, we have identified that the combinatorial expression of clustered protocadherin gammas (γ-PCDHs) is essential in regulating such specificity in the mouse neocortex. Using single-cell sequencing from the 5-prime end, we revealed the combinatorial expression pattern of γ-PCDH variable isoforms in neocortical neurons. Whole-cell patch-clamp recordings demonstrated that increasing the similarity level of this combinatorial pattern in neurons reduced their synaptic connectivity. Our findings reveal a delicate molecular mechanism for assembling the neural network in the mouse neocortex.

To investigate the complications of tourniquet in the clinical application of lower tibiofibular fracture.From June 2018 to September 2019, 33 cases of closed lower tibiofibular fractures (AO type 43A) were treated with plates and screws and were divided into two groups according to whether pueumatic tourniquet was used:16 cases in the observation group, 13 males and 3 females, aged 18 to 69 (38.8±17.0) years, the operation time after injury was (6.9±1.7) days, and tourniquet was not used during operation. There were 17 cases in the control group, 13 males and 4 females, aged from 21 to 71 (43.8±12.4) years, the operation time after injury was (6.5±1.0) days, automatic pneumatic tourniquetwas routinely used in the operation. The operation time, blood loss, postoperative swelling, pain and other complications were compared between two groups.Total of 33 patients were followed up for an average of 15 months. There was no significant difference in operation time and blood loss between two groups (The fracture of lower tibiofibular segment is superficial and easy to be exposed and fixed during operation. In order to avoid tourniquet complications, it is not recommended to use air bag tourniquet routinely or minimize the application time of tourniquet.

Dissecting neural connectivity patterns within local brain regions is an essential step to understanding the function of the brain.

Traditional star image identification methods use adjacent stellar angular distances to form identification features. The missed detection of high-magnitude stars due to noise interference affects their identification accuracy. We propose a star image identification method based on perceptual hash features. The multiple Gaussian distribution superposition constructed by stars within a certain range around the center star forms the unique standard identification image of that star, in which the brightness distribution is determined by stellar magnitudes. The perceptual hash feature of the standard identification image is then used as a standard identification feature. The observation feature is extracted from the identification image as-constructed by the star points extracted from the star image. Finally, the observation feature is matched with the standard feature to identify the star image. Simulation results indicate that the proposed method can efficiently identify star images and effectively prevent missed stars from influencing the matching results.

Background Numerous studies have sought associations between matrix metalloproteinase 9 (MMP-9) polymorphisms and breast cancer risk. However, these studies have yielded conflicting results. Hence, we performed an updated meta-analysis to clarify the effects of four MMP-9 gene polymorphisms (rs3918242, rs2250889, rs3787268, and rs17576) on breast cancer risk. Methods A comprehensive literature search for eligible studies was conducted in five electronic databases, including PubMed, Embase and Web of Science, up to March 1, 2020. Summary odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of associations in random-effects models. For the reduction of type I errors, a trial sequential analysis (TSA) was performed. Results Twenty-one studies (8813 breast cancer cases and 9323 controls) were included in the quantitative analysis. For rs3918242, the overall ORs were significant under allelic comparison (OR A vs. G = 1.34; 95% CI 1.03, 1.74, P = 0.028) and the recessive genetic model (OR AA vs. GG+GA = 1.40; 95% CI 1.06, 1.84, P = 0.016). For rs2250889, the ORs were significant under homozygote comparison (OR GG vs. CC = 2.57; 95% CI 1.22, 5.42, P = 0.013), heterozygote comparison (OR GC vs. CC = 2.48; 95% CI 1.17, 5.23, P = 0.018), and the dominant genetic model (OR GG+GC vs. CC = 2.53; 95% CI 1.23, 5.20, P = 0.012). No associations were observed for rs3787268 or rs17576. The subgroup analyses indicated that the risk effect of the rs3918242 A allele was observed only among Asians. TSA showed that the findings for rs3918242, rs3787268, and rs17576 were robust, but many more patients are needed before definitive conclusions can be made for rs2250889. Conclusion Our meta-analysis suggests that MMP-9 rs3918242, but not rs3787268 and rs17576 polymorphisms, may be risk factors for breast cancer. The effect of rs2250889 needs further confirmation with a larger sample size.

On-demand shared mobility services such as Uber and microtransit are steadily penetrating the worldwide market for traditional dispatched taxi services. Hence, taxi companies are seeking ways to compete. This study mined large-scale mobility data from connected taxis to discover beneficial patterns that may inform strategies to improve dispatch taxi business. It is not practical to manually clean and filter large-scale mobility data that contains GPS information. Therefore, this research contributes and demonstrates an automated method of data cleaning and filtering that is suitable for such types of datasets. The cleaning method defines three filter variables and applies a layered statistical filtering technique to eliminate outlier records that do not contribute to distributions that match expected theoretical distributions of the variables. Chi-squared statistical tests evaluate the quality of the cleaned data by comparing the distribution of the three variables with their expected distributions. The overall cleaning method removed approximately 5% of the data, which consisted of errors that were obvious and others that were poor quality outliers. Subsequently, mining the cleaned data revealed that trip production in Dubai peaks for the case when only the same two drivers operate the same taxi. This finding would not have been possible without access to proprietary data that contains unique identifiers for both drivers and taxis. Datasets that identify individual drivers are not publicly available.

Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo. This system provides a flexible and rapid screening platform for studying complex gene networks and gain-of-function phenotypes in the mammalian brain.

Summary Conversion of glial cells into functional neurons represents a potential therapeutic approach for replenishing neuronal loss associated with neurodegenerative diseases and brain injury. Previous attempts in this area using expression of transcription factors were hindered by the low conversion efficiency and failure of generating desired neuronal types in vivo. Here, we report that downregulation of a single RNA-binding protein, polypyrimidine tract-binding protein 1 (Ptbp1), using in vivo viral delivery of a recently developed RNA-targeting CRISPR system CasRx, resulted in the conversion of Muller glia into retinal ganglion cells (RGCs) with a high efficiency, leading to the alleviation of disease symptoms associated with RGC loss. Furthermore, this approach also induced neurons with dopaminergic features in the striatum and alleviated motor defects in a Parkinson’s disease mouse model. Thus, glia-to-neuron conversion by CasRx-mediated Ptbp1 knockdown represents a promising in vivo genetic approach for treating a variety of disorders due to neuronal loss.

C26H28Cl2N2O3, monoclinic, P21/c (no. 14), a = 13.422(3) Å, b = 7.0011(14) Å, c = 26.249(5) Å, β = 101.06(3)°, V = 2420.8(9) Å3, Z = 4, R gt(F) = 0.0516, wR ref(F 2) = 0.1370, T = 296 K.

This paper takes the ultra-low permeability sandstone reservoir of Jingbian oilfield in Ordos Basin as the research object, analyzes the petrological characteristics, diagenesis, physical characteristics and pore structure characteristics of the reservoir, and carries out reservoir sensitivity evaluation by using rock casting thin sections, X-ray diffraction, and sensitive flow experiments. The research results show that the ultra-low permeability Chang 6 sandstone reservoir has weak velocity sensitivity, medium-weak water sensitivity, weak salt sensitivity, weak alkali sensitivity and strong acid sensitivity; the damage mechanism of reservoir sensitivity mainly depends on the composition of clay minerals and pore structure after diagenesis. The clay mineral content from high to low is chlorite, illite, a small amount of illite / smectite layer, and kaolinite, of which the chlorite content is as high as 75 %; the reservoir has poor physical properties, the types of small hole-thin throat and small hole-fine throat. The reservoir is prone to blockage such as bridge plugging. Therefore, ultra-low permeability sandstone reservoirs are prone to different degrees of sensitivity. The reservoir characteristics are consistent with the reservoir sensitivity evaluation results.

U0126 (1,4-diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene), a widely used mitogen-activated protein kinase kinase (MEK) inhibitor, was found to accelerate voltage-gated K+ channel (KV) inactivation in heterologous cells expressing several types of KV. The goal of this study was to examine whether U0126 at a concentration thought to specifically inhibit MEK signaling also inhibits KV in native neurons of primary cultures or brain slices. U0126 caused a dose-dependent inhibition of both the transient (IA) and sustained (IDR) components of K+ currents in hippocampal neurons. U0126 also exhibited much higher potency on the IA and IDR than the classical KV blockers 4-aminopyridine (4-AP) and tetraethylammonium (TEA). Consistent with its inhibitory effect on KV, U0126 broadened action potential duration, profoundly affected the repolarizing phase, and dramatically reduced firing frequency in response to current pulse injections. Despite the potent and reversible action of U0126 on Kv channels, PD98059, a structurally-unrelated MEK inhibitor, did not induce such an effect, suggesting U0126 may act independently of MEK inhibition. Together, these results raise cautions for using U0126 as a specific inhibitor for studying MEK signaling in neurons; on the other hand, further studies on the blocking mechanisms of U0126 as a potent inhibitor of KV may provide useful insights into the structure-function relationship of KV in general.

Mold shell with higher thermal conductivity was introduced in the article. Reinforced by the carbon fiber, the new type sol silicate mold shell could be made with much less layers while without reducing its strength. Therefore, the new-type mold shell should have higher thermal conductivity. Then measure its thickness, bend strength, analyze the dendritic crystal of the casting manufactured with the new-type mold shell. It was proved that the thickness had reduced by 25% and the bend strength did not decrease. And it performs better in the detection: the dendrite crystals are refined, the primary and secondary dendrite arm space are decreased, the secondary dendrite arms are restrained.

The statistics and analysis of mine gas extraction is very important for mine gas control, which provides a basis for the formulation of mine gas extraction plan. Because of its simple and convenient characteristics, it is favored by the engineering and technical personnel in the coal mine field, and has been widely used, and achieved good results. Based on the statistics of gas drainage in Weijiadi Coal Mine from 2008 to 2018, the characteristics and quantity of gas emission are analyzed according to the statistical results of gas drainage in Weijiadi Coal Mine, and the characteristics of gas emission in the process of driving in the main mining seam are predicted, and the mathematics relations of the length of driving roadway and the quantity of gas emission is obtained.

In view of the coupling problem of gas and coal spontaneous combustion in goaf of 2302 coal mining face in Weijiadi Coal Mine in Jingyuan Coal Area, if the control is not in place, it may cause serious accidents such as gas explosion. By means of fluent simulation, the spontaneous combustion period and oxygen distribution of residual coal in goaf are obtained, and the minimum advancing speed of coal mining face to prevent spontaneous combustion in goaf is calculated to be 1.43m/d. By implementing fire prevention measures such as blocking air leakage in upper and lower corners, grouting and nitrogen injection in goaf, the oxygen concentration in goaf is controlled within 11%, and the spontaneous combustion zone of oxidation is fast. Transferring to asphyxiation zone and preventing the residual coal from firing in goaf are of great significance for ensuring the safety of mine production.

Taking 1302 fully mechanized caving face of Weijiadi Coal Mine as the research object, aiming at the characteristics of large gas emission and frequent gas exceeding limit during mining, this paper analyzed the difficult problems of gas control in working face, determined the theoretical height of caving zone and fissure zone of working face according to the gas cascade control mode formulated by Weijiadi Coal Mine and the principle of pressure relief gas, and inspected the “three zones”. The reasonable horizon and arrangement of drilling holes along the strike of large diameter and high position rocks are determined. The field practice results show that the gas extraction technology of large diameter and high rock trending borehole has good extraction effect, the maximum gas concentration is 26.5% and the maximum gas extraction volume is 14000 m3. It effectively solves the problem of return air flow and upper corner gas exceeding the limit. The gas concentration of return air flow is basically kept at 0.2%∼0.25%, which ensures the safety of mine production.

China is rich in coal resources, especially in Northwest China. However, due to the complex geological conditions, some low permeability and high gas seams often accompany abnormal gas emission during mining, which poses a serious threat to the safety of coal mine. This paper takes East 102 working face of Weijiadi Coal Mine in Jingyuan mining area of Gansu Province as the research object. Aiming at the abnormal gas emission phenomenon during the initial discharge period of working face, the causes of abnormal gas emission are analyzed. The prevention of abnormal gas emission should be carried out from five aspects: strengthening ventilation management, perfecting gas extraction system, perfecting safety monitoring and control system, strengthening power supply management and emergency management on site.

The division of gas geological units in coal mine is the basis of studying the occurrence law of gas geology in coal mine field, which is of great significance to the prediction of regional gas content and gas emission. Taking Weijiadi coalfield in Jingyuan mining area of Gansu Province as an object, this paper divides the coalfield into gas geological units, according to the gas content of coal seams in different regions, targeted gas extraction measures are implemented, gas cascade control is implemented, regional gas abnormal emission is strictly prevented, and mine safety production is ensured.

Abnormal gas emission is a common gas phenomenon in coal mining face of gas mine. In order to prevent gas accidents, on the one hand, gas extraction measures are adopted to reduce or avoid abnormal gas emission; on the other hand, when abnormal gas emission is unavoidable, in order to prevent abnormal gas emission from occurring gas explosion accident by electric spark, non-cost cutting is adopted. Emergency measures such as power supply for quality and safety electrical equipment are taken as the second line of defense. Aiming at the abnormal gas emission phenomenon in East 102 coal face of Weijiadi Coal Mine, in order to avoid the gas explosion accident during the abnormal gas gushing period, the emergency measures for gas abnormal emission were expounded from the aspects of gas electric blockade, power cut and evacuation.

In Weijiadi Coal Mine, the No.2 coal seam is uncovered by the Donger return air shaft. According to the concept of “regional measures first, local measures supplement”, the measures are taken strictly in the outburst seam, the gas pressure P=0.2MPa and the gas content W=1.4729m3/t in the coal seam are determined by preconstruction prediction drilling, and there are no gas dynamic phenomena such as drilling jam and sticking in the drilling process. It is proved that there is no outburst danger, and then it is implemented according to the local “four in one” outburst prevention measures. The maximum gas pressure determined is 0.2 MPa in the working face, and the validity check is still no outburst danger. Finally, safety protection devices are installed in the working face to ensure the No.2 coal seam to be uncovered smoothly by return air shaft.

In view of the frequent occurrence of roof collapse and chipping accidents in the broken surrounding rock roadway in the goaf, grouting reinforcement is applied to the surrounding rock of the ro ent ratio. The field test proves that the selected grouting parameters are reasonable and achieve the purpose of controlling the deformation of the surrounding rock of the roadway.

Stratigraphic sequence contrast and sequence stratigraphic framework are of great significance for the sedimentary phase and sedimentary systems of coal-accumulating basin. In the coal-bearing basin, the variation of the flooding surface controls the migration law of the coal-concentrating centres within the range. Therefore, the sequence comparison work is crucial to find favorable coal-collecting areas. In addition, studying the position of the coal seam in the sequence stratigraphic framework and carrying out the sequence comparison work in the lateral direction can effectively track the change of the position of the coal seam in the sequence stratum and summarize the law of coal seam accumulation and occurrence, which can be better to plan and guide the exploration and development of coal resources in Longdong coalfield.

To explore the effect of a self-made guiding needle of steel wire in guiding the wire through the tibial tunnel for the treatment of avulsion fractures of tibial posterior cruciate ligament with open reduction and wire fixation.From February 2011 to June 2014, a total of 22 patients with avulsion fractures of tibial posterior cruciate ligament underwent surgical treatments were analyzed, including 14 males and 8 females with an average age of 35.6 years old (ranged, 17 to 63 years old). According to Meyers classification, 9 patients were classified as type II, 13 patients were classified as type III. All the patients underwent open reduction and wire fixation with medial knee "L" shape approach. A wire guiding needle was used to guide the wire through the tibial tunnel during operation.With the assistance of wire guidance needles, wires passed through the tibial tunnel rapidly during the operation in all the 22 patients. All the patients were followed up, X-ray imagings 6 months after operation showed the fractures healed well. The average follow-up time in all patients was 6 months (ranged, 6 to 12 months). The averaged Lysholm knee score in 22 knee was 92.7 +/- 3.4. All patients' posterior drawer test were negative.Self-made wire guiding needle can simplify the operation procedures in which the wires pass through the tibial tunnel, shorten the operation time, reduce the surgical trauma and complications, and be worthy of clinical application.

In Ad-hoc wireless network, connectivity is a fundamental issue which restricts the design of system protocol. Based on the theory of stochastic geometry, a connectivity model focused on signal-to-interference (SIR) ratio is set up in presence of Nakagami-m fading and interference. This paper derives a close formula of connectivity probability with interference and Nakagami-m fading which is never obtained in previous works. Two-dimension shot-noise theory in stochastic geometry for interference is well applied. The formula is verified by simulation. The results show that the connectivity is affected by the scatter of users, wireless propagation environment, interference and so on.

In the previous studies, it was revealed that Mitragyna rotundifolia Kuntze (MR) had strong antioxidant activity in vitro. Oxidative stress is considered to play a prominent causative role in the development of various hepatic disorders. n-Butanol extract from M. rotundifolia barks (MRBBU) and leaves (MRLBU) were investigated, and it played a protective role against carbon tetrachloride (CCl4)-induced acute liver injury in mice. The degree of protection has been measured by using biochemical parameters, such as serum glutamic pyruvic transaminase (GPT), serum glutamic oxaloacetic transaminase (GOT), malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue homogenate. The results of the intragastric administration of MRBBU (300, 150 and 75 mg/kg body weight per day, respectively) and MRLBU (300, 150 and 75 mg/kg body weight per day, respectively) on CCL4-induced acute liver injury in mice for 8 days demonstrated that the level of GPT and GOT in each treatment group decreased significantly (P 0.05), but that in the other treatment groups significantly decreased (P 0.05), the level of SOD in other treatment groups significantly increased (P < 0.001, P < 0.01 and P < 0.05, respectively). Results indicated that MR has hepatoprotective effect against CCl4-induced oxidative damage in mice, and the hepatoprotective effect may be correlated with its antioxidant effects. Key words: Mitragyna rotundifolia Kuntze, antioxidant activity, carbon tetrachloride, hepatoprotective effect.

Tailings dam due to earthquake action may lead to severe slippage damage, and setting antiseismic measure is an effective method to prevent damage. Dynamic nonlinear finite element method is used in the efforts to analyze the earthquake reaction and the deformation characteristic. To reduce dam seismic deformation, the reinforcement effect of geotextiles, rock pressure and stone column is studied. Finally, a synthetically antiseismic measure that is composed of geotextiles-reinforced in sub-dam, rock pressure out of starter dam and densification foundation is proposed. It is found that the synthetically antiseismic measure produces better antiseismic effect, and offers a consult for the antiseismic design of tailings dam.

Removed due to plagiarism

It analyses different kinds of risk factors of real estate investment, and establishes a suit of scientific index system about the risk assessment of real estate investment. And this paper not only establishes the risk assessment model of real estate projects in view of fuzzy-synthetical evaluation, but also uses this model to make a risk assessment of Ganzhou Tianyue real estate project, showing the feasibility of the model by examples.

We explored the utility of time-resolved angiography with interleaved stochastic trajectories dynamic contrast-enhanced magnetic resonance imaging (TWIST DCE-MRI), readout segmentation of long variable echo-trains diffusion-weighted magnetic resonance imaging- diffusion-weighted magnetic resonance imaging (RESOLVE-DWI), and echo-planar imaging- diffusion-weighted magnetic resonance imaging (EPI-DWI) for distinguishing between malignant and benign breast lesions. This retrospective analysis included female patients with breast lesions seen at a single center in China between January 2016 and April 2016. Patients were allocated to a benign or malignant group based on pathologic diagnosis. All patients received routine MRI, RESOLVE-DWI, EPI-DWI, and TWIST DCE-T1WI. Variables measured included quantitative parameters (Ktrans, Kep, and Ve), semiquantitative parameters (rate of contrast enhancement for contrast agent inflow [W-in], rate of contrast decay for contrast agent outflow [W-out], and time-to-peak enhancement after contrast agent injection [TTP]) and apparent diffusion coefficient (ADC) values for RESOLVE-DWI (ADCr) and EPI-DWI (ADCe). Receiver-operating characteristic (ROC) curve analysis was used to evaluate the diagnostic utility of each parameter for differentiating malignant from benign breast lesions. A total of 87 patients were included (benign, n = 20; malignant, n = 67). Compared with the benign group, the malignant group had significantly higher Ktrans, Kep and W-in and significantly lower W-out, TTP, ADCe, and ADCr (all P

Flying capacitor multilevel inverters are increasingly used in industry applications. In order to improve the harmonic performance of the output voltage under low modulation index region, the paper presents a novel PWM method for flying capacitor multilevel inverters based on the idea of controlling freedom degree. The novel PWM method can balance the flying capacitor voltage in a certain period. The validity of the novel PWM method is demonstratedby the experimental results.

Bcl-2 is best known for its anti-apoptotic function in a wide variety of cell types. The objective of this study was to investigate the effects of bcl-2 on the types of cell demise in the HeLa/bcl-2 cells induced by H2O2. The HeLa cell expressed stably bcl-2 was established and defined as the HeLa/bcl-2 cell strain, while the cell transfected with the empty expression vector was defined as the HeLa/vector cell strain. MTT assay revealed that the HeLa/bcl-2 cells showed a shorter life span. BrdU incorporation assay indicated that the bcl-2 exerted anti-demise effect on the HeLa/bcl-2 cells at the low concentration of H2O2. However, at the high concentration of H2O2, the death of the HeLa/bcl-2 cells was more than that of the HeLa/vector cells. The flow cytometry demonstrated that H2O2 mainly induced apoptosis in the HeLa/vector cells and elicited necrosis in the HeLa/bcl-2 cells. The addition of celecoxib to the cells treated by H2O2 could increase apoptosis in the HeLa/vector cells and convert necrosis into apoptosis in the HeLa/bcl-2 cells. The higher levels of cellular free radical and GSH were found in the HeLa/bcl-2 cells, but not in the HeLa/vector cells. With 200 microM H2O2 challenge for 48 h, the level of the cellular free radical was increased in the both strains, while the level of the GSH was decreased in the both strains. Celecoxib could reverse the difference between the both strains led by H2O2. Western blotting showed that the expression of COX-2 was always higher in the HeLa/bcl-2 cells than in the HeLa/vector cells under the both of treated and untreated with H2O2, while the level of COX-1 was relative stable in the both strains. These results suggested that the crosstalk between the bcl-2 and the COX-2 pathways could exist, the bcl-2 might up-regulate COX-2 to modify sensitivity to the types of demise in the HeLa/bcl-2 cell.

Determining the degree of synapse formation and elimination is essential for understanding the structural basis of brain plasticity and pathology. We show that in vivo imaging of dendritic spine dynamics through an open-skull glass window, but not a thinned-skull window, is associated with high spine turnover and substantial glial activation during the first month after surgery. These findings help to explain existing discrepancies in the degree of dendritic spine plasticity observed in the mature cortex.

With the size of transistors scaling down, 3D IC packaging emerged as one of the most promising solutions to achieve system integration on the track of Moore's Law. In this article, we demonstrated a sub-system with one 28nm logic device and two 40nm chips on a 600mm2 silicon interposer with Through-Silicon-Via (TSV) integrating 4 layers of high density interconnects. The packages were assembled using our proprietary CoWoS (Chip on Wafer on Substrate) technology that incorporated 270,000 micro-bump (μBump) and 8,700 C4 bumps. Comprehensive reliability characterization and test methods will be presented. It includes copper interconnect reliability of silicon interposer on EM, SM and IMD TDDB with the presence of TSV. μBump EM and TSV EM are characterized to provide a design guideline for maximum current carrying capability; the EM test methodology was also used to optimize the integrated process, e.g. TSV copper plating, μBump joint, and interface treatment of TSV revealing. Package process optimization, bill of material selectio n and qualificatio n we r e conducted on a 28nm Cu/ELK (Extreme low-K) test vehicle taking Chip-Package-Interaction into consideration. Not only component level package reliability tests were performed, board level Thermal Cycling, Power Cycling and Mechanical Shock tests were also executed to understand the reliability margin and potential failure modes in field use condition. The typical failure modes and mechanisms will be discussed. CoWoS technology is eventually successfully developed with enhanced reliability. The results highlight the importance of a highly integrated 3D IC technology from silicon wafer process to assembly packaging. This work shows that in the new paradigm of 3D IC integration, Si Foundry is positioned at a unique leadership to manage the innovation in Si processes and the improvement of Si assembly operating life.

A fundamental feature of the mammalian neocortex is its columnar organization. In the visual cortex, functional columns consisting of neurons with similar orientation preferences have been characterized extensively, but how these columns are constructed during development remains unclear. The radial unit hypothesis posits that the ontogenetic columns formed by clonally related neurons migrating along the same radial glial fibre during corticogenesis provide the basis for functional columns in adult neocortex. However, a direct correspondence between the ontogenetic and functional columns has not been demonstrated. Here we show that, despite the lack of a discernible orientation map in mouse visual cortex, sister neurons in the same radial clone exhibit similar orientation preferences. Using a retroviral vector encoding green fluorescent protein to label radial clones of excitatory neurons, and in vivo two-photon calcium imaging to measure neuronal response properties, we found that sister neurons preferred similar orientations whereas nearby non-sister neurons showed no such relationship. Interestingly, disruption of gap junction coupling by viral expression of a dominant-negative mutant of Cx26 (also known as Gjb2) or by daily administration of a gap junction blocker, carbenoxolone, during the first postnatal week greatly diminished the functional similarity between sister neurons, suggesting that the maturation of ontogenetic into functional columns requires intercellular communication through gap junctions. Together with the recent finding of preferential excitatory connections among sister neurons, our results support the radial unit hypothesis and unify the ontogenetic and functional columns in the visual cortex.

The chemical composition of some glass samples in Jin and Yuan Dynasty were analyzed by EDXRF, to discuss the technological character of these glass samples. The result shows that the chemical composition of these glass samples is very complex. According to the chemical composition, these glass samples include K2-O-CaO-SiO2, Na2O-CaO-SiO2 and K2O-CaO-PbO-SiO2 glass, and a crystal SiO2 sample which was once considered a glass sample. The reason for the color of all blue glass samples is the existence of CuO, but not CoO.