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The addition of green tea leaves fractions < 0.315 mm as afiller to urea-formaldehyde resin to reduction of emission of formaldehyde from three-layer birch plywood was investigated. Moreover, other properties such as bonding quality and water resistance of plywood were investigated. It was found that green tea had an ability to absorb formaldehyde. This phenomenon was caused by phenolic compounds contained in green tea leaves. The addition of green tea in the amount of 20% and 25% resulted in a decrease in formaldehyde emission fromplywood comparing to the reference sample. Thebonding quality test showed that the addition of tea leaves as a filler did not affect thestrength properties of the tested bond lines.

STUDY QUESTION Are there specific autoantibody profiles in patients with endometriosis that are different from those in controls? SUMMARY ANSWER This study did not reveal a significantly higher prevalence of autoantibodies in the studied groups of patients. WHAT IS KNOWN ALREADY Various inflammatory factors are postulated to be involved in the pathomechanisms of endometriosis, and a potential link exists with autoimmune diseases, which may also play an important role. As the diagnosis of endometriosis remains invasive, it can only be confirmed using laparoscopy with histopathological examination of tissues. Numerous studies have focused on identifying useful biomarkers to confirm the disease, but without unequivocal effects. Autoantibodies are promising molecules that serve as potential prognostic factors. STUDY DESIGN, SIZE, DURATION A multicentre, cross-sectional study was conducted over 18 months (between 2018 and 2019), at eight Departments of Obstetrics and Gynaecology in several cities across Poland on 137 patients undergoing laparoscopic examination for the diagnosis of endometriosis. PARTICIPANTS/MATERIALS, SETTINGS, METHODS During laparoscopy, we obtained plasma samples from 137 patients and peritoneal fluid (PF) samples from 98 patients. Patients with autoimmune diseases were excluded from the study. Autoantibody profiling was performed using HuProt v3.1 human proteome microarrays. MAIN RESULTS AND THE ROLE OF CHANCE We observed no significant differences in the expression of autoantibodies in the plasma or PF between the endometriosis and control groups. The study revealed that in the PF of women with Stage II endometriosis, compared with other stages, there were significantly higher reactivity signals for ANAPC15 and GABPB1 (adj. P 1 in both cases). Comparison of the luteal and follicular phases in endometriosis patients revealed that levels of NEIL1 (adj. P LIMITATIONS, REASONS FOR CAUTION Although this study was performed using the highest-throughput protein array available, it does not cover the entire human proteome and cannot be used to study potentially promising post-translational modifications. Autoantibody levels depend on numerous factors, such as infections; therefore the autoantibody tests should be repeated for more objective results. WIDER IMPLICATIONS OF THE FINDINGS Although endometriosis has been linked to different autoimmune diseases, it is unlikely that autoimmune responses mediated by specific autoantibodies play a pivotal role in the pathogenesis of this inflammatory disease. Our study shows that in searching for biomarkers of endometriosis, it may be more efficient to use higher-throughput proteomic microarrays, which may allow the detection of potentially new biomarkers. Only research on such a scale, and possibly with different technologies, can help discover biomarkers that will change the method of endometriosis diagnosis. STUDY FUNDING/COMPETING INTEREST(S) This study was funded by a grant from the Polish Ministry of Health (grant no. 6/6/4/1/NPZ/2017/1210/1352). It was also funded by the Estonian Research Council (grant PRG1076) and the Horizon 2020 Innovation Grant (ERIN; grant no. EU952516), Enterprise Estonia (grant no. EU48695), and MSCA-RISE-2020 project TRENDO (grant no. 101008193). The authors declare that there is no conflict of interest. TRIAL REGISTRATION NUMBER N/A.

The goal of this article is to assess the views of university students on their mobility in the context of the COVID-19 pandemic. To achieve this goal, a survey was conducted to analyze changes in student mobility patterns before and during the pandemic. The research was conducted in March 2021, during the pandemic, and for its purpose, information was collected on various aspects of student mobility, including the form of study, destination, mode of transportation, and shopping location. The sample group consisted of university students from different backgrounds, and various features, including age, gender, residence, and driver's license status, were considered. In addition, the study examined the impact of COVID-19 on student mobility. The study found that the pandemic has significantly affected student mobility, with a shift towards remote or hybrid learning and preference for cars, bicycles, and walking over public transportation in bigger towns while in smaller towns and rural areas, student mobility behavior has remained relatively unchanged.

Norbert Morciniec is the author of the first Polish-Dutch and Dutch-Polish dictionary, which was published in the 1970s. Ever since, students have seemed to be using it. This article seeks to answer the question of what use value dictionaries published nearly fifty years ago have. Dutch entries beginning with the letters E and F were analyzed. The research questions were: (1) Are these words still present in Dutch in 2022? (2) Has their form or meaning changed? The conclusions show that old dictionaries can still sometimes be helpful; however, it is better to use more up-to-date ones. This in no way undermines the role and importance of Professor Morciniec’s dictionary for the first Polish generations of Dutch speakers.

Word formation seems to be a branch of linguistics with an already well-establishedterminology, but it becomes clear that it is not always the case when one considersdifferences that occur in publications in this area. The term “affixoid” is not new, yetlinguists argue whether it is necessary at all. Nevertheless, there undoubtedly exists anarea on the border of derivation and compounding and, accordingly, there are constructionsthat consist of morphemes that can be classified as neither a stem nor an affix. This, inturn, means that the constructions in question are neither derivates nor compounds. Thepresent article is to play a role of an impulse to initiate discussion on the term “affixoid.” Based on the material from Danish, it aims to present arguments in favour of using theterm “semi-stem” in order to characterize such morphemes and constructions in whichthey occur.

The evolution of costly traits such as deer antlers and peacock trains, which drove the formation of Darwinian sexual selection theory, has been suggested to both reflect and affect patterns of genetic variance across the genome, but direct tests are missing. Here, we used an evolve and resequence approach to reveal patterns of genome-wide diversity associated with the expression of a sexually selected weapon that is dimorphic among males of the bulb mite, Rhizoglyphus robini. Populations selected for the weapon showed reduced genome-wide diversity compared to populations selected against the weapon, particularly in terms of the number of segregating non-synonymous positions, indicating enhanced purifying selection. This increased purifying selection reduced inbreeding depression, but outbred female fitness did not improve, possibly because any benefits were offset by increased sexual antagonism. Most single nucleotide polymorphisms (SNPs) that consistently diverged in response to selection were initially rare and overrepresented in exons, and enriched in regions under balancing or relaxed selection, suggesting they are probably moderately deleterious variants. These diverged SNPs were scattered across the genome, further demonstrating that selection for or against the weapon and the associated changes to the mating system can both capture and influence genome-wide variation.

The study presents a new triangulation-based workflow to assess the degree of parallelism between geological surfaces. This workflow consists of producing and analyzing angular distance distributions as well as conducting spatial analysis using grid maps applicable for subsurface environments with sparse data. We tested our approach using a set of interfaces from Kraków-Silesian Homocline, a geological unit with preferred subhorizontal dip to NE. The pairs of interfaces for angular distance measurements can be divided into two groups: i) separating only Jurassic homocline-related units and ii) separating Jurassic homocline-related units from homocline-unrelated units. To observe potential differences for these two groups, we used bootstrap methods and estimated confidence intervals for summary statistics. In our case, the mean of angular distances turned out to be in general smaller for the pair separating only homocline-related Jurassic sediments. The results also show that the method can be more sensitive to the identification of small-scale structures which are developed only in some of the analyzed surfaces. We provided open-source and freely available computer code to allow reproducibility of the results.

This article pertains to the heritage of the ancient Rome in the Western European cities as a theme of cultural tourism. The main objective is to describe this heritage and to present it as a tourist value of particularly high historical and cultural worth. The main goal is to develop the authors’ concept of a cultural heritage route named the Rhineland Route of the Roman Civilization that includes 11 cities where 30 selected monuments of legacy of the ancient Roman civilization have been depicted. A cartographic method, namely the signature one, has been applied in the article. It was used to mark, among other locations, towns with Roman roots on a map of the Rhineland, showing a proposed route through the ancient Roman monuments. The heritage of the ancient Rome can serve to raise historical and cultural awareness at many levels – from local and regional to international ones. This historical legacy has a real impact on the tourist attractiveness in all the characterised cities, being well-adapted and attractively developed for tourist use., Artykuł dotyczy dziedzictwa starożytnego Rzymu w miastach zachodnioeuropejskich jako motywu turystyki kulturowej. Celem pracy jest charakterystyka tegoż dziedzictwa i ukazanie go jako waloru turystycznego o szczególnie dużej wartości historyczno-kulturowej. Głównym zadaniem jest opracowanie koncepcji autorskiego szlaku dziedzictwa kulturowego „Nadreński Szlak Cywilizacji Rzymskiej”, który na trasie uwzględnia 11 miast, a w nich 30 wybranych zabytków spuścizny cywilizacji starożytnego Rzymu. W artykule zastosowano jedną z metod kartograficznych, sygnaturową. Posłużyła ona m.in. do zaznaczania na mapie miast o rzymskich korzeniach w Nadrenii, jednocześnie pokazując proponowaną trasę przez starożytne rzymskie zabytki. Dziedzictwo starożytnego Rzymu może służyć podnoszeniu świadomości historycznej i kulturowej na wielu poziomach – od lokalnych i regionalnych po międzynarodowe. Dziedzictwo to w scharakteryzowanych w artykule miastach ma realny wpływ na ich atrakcyjność turystyczną, a także jest dobrze przystosowane i atrakcyjnie zagospodarowane do użytku turystycznego.

Supplementary Table 2 from Gender Disparities in the Tumor Genetics and Clinical Outcome of Multiple Myeloma

Supplementary Table 3 from Gender Disparities in the Tumor Genetics and Clinical Outcome of Multiple Myeloma

Supplementary Methods, References, Legends for Table 1 and Figure 1 from Gender Disparities in the Tumor Genetics and Clinical Outcome of Multiple Myeloma

Supplementary Table 1 from Gender Disparities in the Tumor Genetics and Clinical Outcome of Multiple Myeloma

Sexual selection and sexual antagonism are important drivers of eco-evolutionary processes. The evolution of traits shaped by these processes depends on their genetic architecture, which remains poorly studied. Here, implementing a quantitative genetics approach using diallel crosses of the bulb mite, Rhizoglyphus robini, we investigated the genetic variance that underlies a sexually selected weapon that is dimorphic among males and female fecundity. Previous studies indicated that a negative genetic correlation between these two traits likely exists. We found male morph showed considerable additive genetic variance, which is unlikely to be explained solely by mutation-selection balance, indicating the likely presence of large-effect loci. However, a significant magnitude of inbreeding depression also indicates that morph expression is likely to be condition-dependent to some degree and that deleterious recessives can simultaneously contribute to morph expression. Female fecundity also showed a high degree of inbreeding depression, but the variance in female fecundity was mostly explained by epistatic effects, with very little contribution from additive effects. We found no significant genetic correlation, nor any evidence for dominance reversal, between male morph and female fecundity. The complex genetic architecture underlying male morph and female fecundity in this system has important implications for our understanding of the evolutionary interplay between purifying selection and sexually antagonistic selection.

There is a growing trend in the scientific world to publish papers in English. Describing research in the field of technology or medicine in English is justified, because it allows for faster verification of results and their application by scientists in any part of the world, and thus their faster use by ordinary people. It is questionable, however, whether it makes sense to write about a language – especially one as small as Dutch – in the language of Shakespeare. Dutch still lends itself very well to scientific argument, as the publications in this volume prove.

(1) Background: The aim of this study was to assess the outcomes for patients who underwent total colectomy (TC) as a part of surgery for ovarian cancer (OC). (2) Methods: We performed a retrospective analysis of 1636 OC patients. Residual disease (RD) was reported using Sugarbaker’s completeness of cytoreduction score. (3) Results: Forty-two patients underwent TC during primary debulking surgery (PDS), and four and ten patients underwent TC during the interval debulking surgery (IDS) and secondary cytoreduction, respectively. The median overall survival (mOS) in OC patients following the PDS was 45.1 months in those with CC-0 (21%) resection, 11.1 months in those with CC-1 (45%) resection and 20.0 months in those with CC-2 (33%) resection (p = 0.28). Severe adverse events were reported in 18 patients (43%). In the IDS group, two patients survived more than 2 years after IDS and one patient died after 28.6 months. In the recurrent OC group, the mOS was 6.9 months. Patient age above 65 years was associated with a shortened overall survival (OS) and the presence of adverse events. (4) Conclusions: TC as a part of ultra-radical surgery for advanced OC results in high rates of optimal debulking. However, survival benefits were observed only in patients with no macroscopic disease.

Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial–mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical procedures in eight clinical centers. Quantitative ZEB1 and ZEB2 levels analyses were performed using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). No significant differences were observed in ZEB1 levels in any of the subanalyses nor any differences regarding ZEB2 levels between patients with and without endometriosis. Plasma ZEB2 levels were significantly higher among patients with infertility compared to fertile women (16.07 ± 12.70 ng/L vs. 12.07 ± 11.92 ng/L; p < 0.04). Both ZEB1 and ZEB2 do not seem to have a significant value in the initial diagnosis of endometriosis as a single marker. The differences in ZEB2 plasma levels between patients with and without infertility indicate the possibility of EMT dysregulation in the pathogenesis of adverse fertility outcomes.

The evidence of poly (ADP-ribose) polymerase (PARP) association with the immune response could be coherent with the immunological theory of endometriosis and suggests the possibility of a new research direction. The aim of the study was to evaluate the levels of PARP in plasma and peritoneal fluid of patients with and without endometriosis. It was a multicenter, cross-sectional study. Plasma and peritoneal fluid samples were collected from patients with and without endometriosis during planned laparoscopic procedures in eight clinical centers. In total, 84 samples of plasma and 84 samples of the peritoneal fluid were included in the final analyses. Double-antibody sandwich enzyme-linked immunosorbent assay was performed in order to assess levels of PARP in collected samples. No statistically significant differences regarding the detected levels of PARP in plasma and peritoneal fluid comparing patients with and without endometriosis were observed. Patients with a history of infertility had significantly higher plasma PARP concentrations (p = 0.04). We have not observed the potential role of PARP concentration levels in plasma nor peritoneal fluid as an endometriosis biomarker. We have determined an association between a higher plasma PARP concentration and a history of infertility.

The article concerns constructions such as et herrens år in Danish where the first of the nouns is in the definite form (with a suffixed definite article), in the genitive case and functions as an attribute and not a determiner in relation to the following noun. The determiner (a definite or indefinite article, demonstrative pronoun, etc.), on the other hand, is not linked directly to the following noun but to the one that occurs in the last position of the whole phrase. This stands in contrast to the regular structure of the noun phrase where the determiner refers to the directly following noun (e.g., en bils ejer). In the case of the construction discussed here, however, the determiner refers to the last noun in the phrase (e.g., dette herrens år). Moreover, adjectives can occur before both nouns, but they will always refer to the last noun (e.g., en vældig kræfternes udfoldelse). The work discusses both the grammatical and semantical aspects of the construction. As demonstrated in the article, the construction should not be treated from the perspective of word formation, as it is rather an example of an attributive use of a definite noun in the genitive. Secondly, it is also interesting to point out that the semantic relations within the construction do not fully overlap with the grammatical relations, as from the semantic point of view the determiner, the adjectives, as well as the attributive noun, refer to the main noun. Thirdly, and most importantly, there are strong indications that here we are not dealing with just a few rare examples of a more literary style or certain fixed expressions, but with a regular grammatical construction. To conclude with certainty, however, it is necessary to complement this exploratory research with a corpus study, and preferably also a survey that could reveal the native speakers’ perception of the construction.

Background and Objectives: Endometriosis is a hormone-dependent chronic inflammatory disease with serious reproductive and general health consequences. It is viewed as a multifactorial problem, consisting of matters related to altered immunity and genetics. In this study, we determined the correlation between endometriosis and allergic and autoimmune diseases in patients at reproductive age. Materials and Methods: Online surveys distributed through websites related to gynecological problems. The questionnaire was composed of 63 single and multiple-choice questions concerning the course of endometriosis, diet, and allergic and autoimmune diseases. The obtained data were assessed using statistical tests. Results: 501 female patients (mean age 31.1 SD = 6.8) were included in the study. The control group (n = 155) consisted of healthy females, whereas the study group (n = 346) consisted of female patients with endometriosis; each group was subdivided according to allergy status. There were statistically significant differences between groups for the following: positive family history of endometriosis (p = 0.0002), onset of allergic symptoms (p = 0.0003), frequency and duration of abdominal pain (p = 0.00625), and defecation disorders (p = 0.0006). Asthma was less common in the study group (p = 0.00611). The group of patients with endometriosis and allergies had a high median of consumption of red meat (p = 0.0143), fish (p = 0.0016), and dairy products (p = 0.0001). Conclusions: Endometriosis did not affect autoimmune diseases and their courses. Patients with diagnosed endometriosis presented allergy symptoms much earlier than the healthy patients. The consumption of dietary products such as soya products, red meat, and alcohol had an influence on the occurrence of endometriosis.

The primary aim of our study was to analyse the impact of the lymph node ratio (LNR) and extracapsular involvement (ECI) on the prognosis of endometrial cancer (EC) patients.We carried out a retrospective analysis of 886 patients surgically treated for EC between 2000 and 2015. In the subgroup of patients with lymph node metastases (LNM), we evaluated the impact of the number and localization of the LNM, LNR, and ECI on patients' overall survival (OS).In the group of patients with LNM, 0.3 was the optimal LNR cut-off for differentiating between short- and long-term survivors [HR = 2.94 (95% CI: 1.49-5.80)]. Patients with a LNR ≥ 0.3 had a significantly shorter OS period (35.0 months, range 0.2-175 months) compared to patients with a LNR0.3 [median OS - mOS, was 143, range 15-169 months; (Our results suggest that when LNM are found, the long-term outcomes of EC patients are worse in those who have a LNR ≥ 0.3, the presence of ECI, and a more advanced extension of the primary tumour.

This companion datasetrelates to the manuscript "A new methodology using borehole data to measure angular distances between geological interfaces", by Michał P. Michalaka,b,1, Paweł Marzecb,2, Filip Turobośc,3, Paulina Leonowiczd,4, Lesław Tepera,5, Paweł Gładkie,6, Michael J. Pyrczf,7, Mariusz Szubertg,8 1 Michał Michalak devised the project, wrote the manuscript, performed the computations and discussed the results. 2 Paweł Marzec conducted the geological interpretation and discussed the results. 3 Filip Turoboś conducted the statistical analysis. 4 Paulina Leonowicz prepared the chapter about stratigraphy. 5 Lesław Teper prepared the chapter about regional geology. 6 Paweł Gładki participated in the study conceptualisation (discussion about distance functions) 7 Michael Pyrcz discussed the applications of the method and revised the statistical section. 8 Mariusz Szubert was responsible for the data acquisition. The archive contains the input and processed data. The input data contains XYZ coordinates of points documenting the investigated interfaces. The output files contains calculated orientations and coordinates of vectors. The output filescan be processed in RStudio.

Celem publikacji jest przedstawienie Nowych Gut jako mazurskiej wsi turystycznej oraz zmian zachodzących w krajobrazie kulturowym tej wsi, spowodowanych rozbudową infrastruktury turystycznej, w związku z rozwojem funkcji turystycznej. Metodyka pracy oparta została na inwentaryzacji infrastruktury turystycznej, dokumentacji fotograficznej krajobrazu kulturowego Nowych Gut, opracowaniu dokumentacji kartograficznej infrastruktury turystycznej (z wykorzystaniem OSM i QGIS) oraz wywiadach z mieszkańcami wsi. W latach 20. XX w. Nowe Guty znane były jako atrakcyjne letnisko nad Śniardwami. Turyści mogli tam dojechać pociągiem i korzystać z noclegów w dwóch gospodach. Intensywny rozwój funkcji turystycznej nastąpił po 1990 r. Obecnie jest to wieś turystyczna z dobrze rozwiniętą bazą noclegową. W przestrzenni turystycznej Śniardw wyróżnia ją krajobraz kulturowy, którego elementem jest historyczny układ ruralistyczny wsi mazurskiej kształtowany od poł. XV w. Nowe Guty są wsią typu ulicówka, z charakterystyczną zabudową z wykorzystaniem polodowcowych otoczaków i głazów narzutowych. Infrastruktura turystyczna nie zatarła tych cech. Nowe pensjonaty wkomponowano w krajobraz wsi. Pokoje gościnne urządzono w zaadaptowanych na ten cel dawnych oborach i stodołach. Na obrzeżach historycznej części wsi budowane są domy letniskowe i tzw. drugie domy, jako miejsca rekreacji i wypoczynku świątecznego. Krajobraz turystyczny Nowych Gut odzwierciedla tradycję miejsca, ponieważ zapisany jest w nim historyczno-kulturowy rozwój tej wsi, jest efektem relacji człowiek-turysta–przestrzeń geograficzna w aspekcie przyrodniczym oraz kulturowym. Jest to krajobraz harmonijny i zrównoważony.

SEO – synchronous endometrial ovarian cancer is a well-known phenomenon, which has for years been managed as two primary independent cancers. The results of recent molecular studies, especially next-generation sequencing, suggest that the condition should be regarded as a continuum, with its origin probably lying in the endometrium or endometrial foci. It has been found that 0.7% to 1.0% of endometriosis patients may develop malignant lesions. Although SEO is being increasingly studied, diagnostics and treatment still leave many questions. The most important thing is to improve the diagnosis with rapid and simple detection. A few molecular methods are already known, but genetic diagnostic, still remains unclear. Old criteria implemented by Scully in 1998 should be nowadays complemented by immunohistochemical staining of estrogen and progestin receptors, bcl2 antibodies and molecular analyses of genes: B-catenin, PTEN, KRAS, TP53, PIK3CA and microsatellite instability. Will genetic diagnostics preserve fertility in young patients with SEO? This paper reviews relevant literature to determine a strategy for distinguishing between SEO and metastatic cancers, and presents management options for patients with SEO.

Krajoznawstwo było i jest związane z edukacją, zwłaszcza geograficzną. Krajoznawstwo w szkole utożsamiane jest głównie z wycieczkami, których celem jest poznanie regionu lub kraju ojczystego. Wzbogacanie wiedzy krajoznawczej połączone jest z kształtowaniem postawy patriotycznej. W szczególności odnosi się to małej ojczyzny, jej poznawania oraz działalności krajoznawczej na jej rzecz. Mała ojczyzna jest jednym z edukacyjnych celów szczegółowych nauczania geografii w szkole podstawowej. Temat „Moja mała ojczyzna” przewidziany jest klasie siódmej. W publikacji przedstawiono propozycję realizacji tematu „Moja mała ojczyzna” opartego na WebQueście oraz krajoznawczej interpretacji dziedzictwa małej ojczyzny. Tok WebQuestu składa się z pięciu elementów. Trzem grupom uczniów przydzielone będą trzy zadania, których wynikiem będzie dokumentacja krajoznawcza małej ojczyzny, składająca się z opisu krajoznawczego, mapy oraz krajoznawczych opisów dziedzictwa małej ojczyzny. Jest to zadanie integrujące opis małej ojczyzny z wiedzą nie tylko z zakresu geografii, ale też innych przedmiotów np. biologii, historii, wiedzy o społeczeństwie, j. polskiego, fizyki i chemii. Połączenie zajęć lekcyjnych z krajoznawstwem wyzwala emocjonalny związek ucznia z małą ojczyzną (ojcowizną) i jej dziedzictwem przyrodniczym i kulturowym, wzbudza ciekawość, chęć poznawania świata, co przekłada się na jego aktywizowanie i kreatywność w edukacji szkolnej.

W artykule przedstawiono projekt wycieczki geograficzno-krajoznawczej, która łączy edukację geograficzną z krajoznawstwem. Na tym przykładzie oparto tezę, że metoda projektu geograficzno-krajoznawczego inspiruje i aktywizuje uczniów do zdobywania wiedzy poprzez entuzjazm i pasję poznania. To powoduje, że uczeń podejmuje rolę odkrywcy, a podczas wycieczki, jako geograf i przewodnik, dzieli się wiedzą i objaśnia walory i atrakcje turystyczne. Projekt poszerza i pogłębia wiedzę z zakresu geografii regionalnej oraz historii Polski. Jego realizację przewidziano w klasie siódmej szkoły podstawowej, podczas zajęć pozalekcyjnych w ramach pracy w szkolnym kole geograficznym lub klubie krajoznawczo-turystycznym.

Women may present with psychiatric disorders during pregnancy, normal labor, following delivery by caesarean section, or in the postpartum period. The accumulating evidence suggests that these disorders may be due to changes in immune responses. During pregnancy complications such as the prolongation of cervical ripening or descent, placental abruption, premature labor, and preeclampsia increase the risk of postpartum psychiatric disorders. Women may exhibit depression and postpartum psychosis following either normal birth or caesarean section. Since psychiatric disorders like schizophrenia, major depression, and bipolar disorder are associated with both alterations in the immune response and changes in immune cell subpopulations, in this study we have chosen to examine whether the psychiatric disorders in women during labor or postpartum also lead to aberrant immune responses.

This essay is a plea for more careful use of Dutch language, in particular by translators and teachers of Dutch. In particular, the spelling always proves to be a difficult stumbling block, and not just for second-language or foreign-language learners. The essay examines the problem of compound words and mistakes made by professional language practitioners of Dutch when writing these words or not.

The purpose of this article was to show basic principles, acquisition, advantages, disadvantages, and clinical applications of intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI). IVIM MRI as a method was introduced in the late 1980s, but recently it started attracting more interest thanks to its applications in many fields, particularly in oncology and neuroradiology. This imaging technique has been developed with the objective of obtaining not only a functional analysis of different organs but also different types of lesions. Among many accessible tools in diagnostic imaging, IVIM MRI aroused the interest of many researchers in terms of studying its applicability in the evaluation of abdominal organs and diseases. The major conclusion of this article is that IVIM MRI seems to be a very auspicious method to investigate the human body, and that nowadays the most promising clinical application for IVIM perfusion MRI is oncology. However, due to lack of standardisation of image acquisition and analysis, further studies are needed to validate this method in clinical practice.

Introduction A key survival prognosis factor for patients treated for ovarian cancer is complete cytoreductive surgery where all macroscopic neoplastic implants, including enlarged metastatic lymph nodes, are removed. We presume that investigating the involvement of the lymphatic system can result in a more individualized approach to the treatment of ovarian cancer patients. The main aim of our study was to analyze the relationship between the presence, number and types of lymph node metastases and ovarian cancer patient prognosis. Material and methods We carried out a retrospective analysis of patients who underwent cytoreduction due to primary ovarian cancer, between 2010 and 2015. We analyzed the number of metastatic lymph nodes, the lymph node ratio defined as the ratio of the number of metastatic lymph nodes to the total number of lymph nodes removed, extracapsular involvement, and the histopathological pattern of metastases. Results The study group included 651 patients. Of these, 377 had lymphadenectomy, 144 presented with lymph node metastases, and 233 had no lymph node metastases. We also included a group of 274 patients who did not have lymphadenectomy. Patients with more than 4 metastatic lymph nodes and a lymph node ratio of ≥ 0.1 had significantly poorer overall survival. Extracapsular involvement had no relation to patient overall survival. Multivariant survival analysis indicated that a lymph node ratio of ≥ 0.1 was an independent predictor of poor survival. Conclusions The analysis of lymph node metastases in ovarian cancer patients can have predictive value for patient overall survival.

A relation between Orthorexia Nervosa (ON) and increased frequency of physical activity has been put in evidence by recent studies. It is well known that intense physical exercises are typically related to eating disorders, but its relationship with ON is still a subject of debate. Other transdiagnostic features could be necessary to conceptualize and understand ON; in this way, low self-esteem is related to eating behavior but is not still extensively investigated in ON, and, to date, data are so heterogeneous that they do not allow us to understand if this is a psychological feature somehow associated with ON. The current study aimed to assess whether disordered eating attitudes, self-esteem, and physical activity are associated with ON in young adults from Poland and Italy. Moreover, we investigated the differences by comparing lower and higher ON levels related to disordered eating attitudes, self-esteem, and physical activity. Our results indicated that a great concern about dieting significantly predicted problems associated with healthy eating, knowledge about healthy eating, and feeling positive about healthy eating. In addition, young adults with a high level of ON demonstrated higher levels of disordered eating attitudes and vigorous-intensity physical activity than young adults with a low level of ON. Future studies are needed to assess the direct effect of physical activity and self-esteem on ON.

Adenomyosis is a common benign gynecological condition, defined as an extension of endometrial tissue into the myometrium. Some studies suggest that adenomyosis could be a favorable prediction factor associated with survival outcomes in endometrial cancer. The aim of our systematic review was to investigate the current knowledge regarding adenomyosis and a possible molecular mechanism of carcinogenesis in adenomyotic lesions. In addition, the long-term prognosis for patients with endometrial cancer and coexisting adenomyosis (and endometriosis) was a key point of the research. The current literature was reviewed by searching PubMed, using the following phrases: “adenomyosis and endometrial cancer” and “malignant transformation of adenomyosis”. According to the literature, genetic mutations, epigenetic changes, and inactivation of specific tumor suppressor genes in adenomyosis are still poorly understood. Data regarding the influence of adenomyosis on survival outcomes in endometrial cancer seem to be contradictory and require further clinical and molecular investigation.

„Kultura Współczesna” 120, 4 (2022), pp. 96-109

Background and Objectives: Endometriosis is one of the most common gynecological disorders in women of reproductive age. Causing pelvic pain and infertility, it is considered one of the most serious health problems, being responsible for work absences or productivity loss. Its diagnosis is often delayed because of the need for an invasive laparoscopic approach. Despite years of studies, no single marker for endometriosis has been discovered. The aim of this research was to find an algorithm based on symptoms and laboratory tests that could diagnose endometriosis in a non-invasive way. Materials and Methods: The research group consisted of 101 women hospitalized for diagnostic laparoscopy, among which 71 had confirmed endometriosis. Data on reproductive history were collected in detail. CA125 (cancer antigen-125) level and VEGF1(vascular endothelial growth factor 1) were tested in blood samples. Among the used statistical methods, the LASSO regression—a new important statistical tool eliminating the least useful features—was the only method to have significant results. Results: Out of 19 features based on results of LASSO, 7 variables were chosen: body mass index, age of menarche, cycle length, painful periods, information about using contraception, CA125, and VEGF1. After multivariate logistic regression with a backward strategy, the three most significant features were evaluated. The strongest impact on endometriosis prediction had information about painful periods, CA125 over 15 u/mL, and the lowest BMI, with a sensitivity of 0.8800 and a specificity of 0.8000, respectively. Conclusions: Advanced statistical methods are crucial when creating non-invasive tests for endometriosis. An algorithm based on three easy features, including painful menses, BMI level, and CA125 concentration could have an important place in the non-invasive diagnosis of endometriosis. If confirmed in a prospective study, implementing such an algorithm in populations with a high risk of endometriosis will allow us to cover patients suspected of endometriosis with proper treatment.

Human protein Yin Yang 1 (YY1) controls the transcription of hundreds of genes both positively and negatively through interactions with a wide range of partner proteins. Results presented here from proteolytic sensitivity, calorimetry, circular dichroism, fluorescence, NMR, size-exclusion chromatography, SELEX, and EMSA show that purified YY1 forms dimers via its disordered N-terminal region with strong zinc-ion concentration dependence. The YY1 dimer is shown to bind tandem repeats of a canonical recognition DNA sequence with high affinity, and analysis of human YY1 regulatory sites shows that many contain repeats of its recognition elements. YY1 dimerization may compete with partner protein interactions, making control by zinc ion concentration a previously unrecognized factor affecting YY1 gene regulation. Indeed, YY1 is known to be important in many pathogenic processes, including neoplasia, in which zinc ion concentrations are altered. The present results incentivize studies in vivo or in vitro that explore the role of zinc ion concentration in YY1-mediated gene expression.

Background: lipid nanoparticle (LNP) encapsulated self-amplifying RNA (saRNA) is well tolerated and immunogenic in SARS-CoV-2 seronegative and seropositive individuals aged 18–75. Methods: a phase 2a expanded safety and immunogenicity study of a saRNA SARS-CoV-2 vaccine candidate LNP-nCoVsaRNA, was conducted at participating centres in the UK between 10th August 2020 and 30th July 2021. Participants received 1 μg then 10 μg of LNP-nCoVsaRNA, ∼14 weeks apart. Solicited adverse events (AEs) were collected for one week post-each vaccine, and unsolicited AEs throughout. Binding and neutralisating antiSARS-CoV-2 antibody raised in participant sera was measured by means of an anti-Spike (S) IgG ELISA, and SARS-CoV-2 pseudoneutralisation assay. (The trial is registered: ISRCTN17072692, EudraCT 2020-001646-20). Findings: 216 healthy individuals (median age 51 years) received 1.0 μg followed by 10.0 μg of the vaccine. 28/216 participants were either known to have previous SARS-CoV2 infection and/or were positive for anti-Spike (S) IgG at baseline. Reactogenicity was as expected based on the reactions following licensed COVID-19 vaccines, and there were no serious AEs related to vaccination. 80% of baseline SARS-CoV-2 naïve individuals (147/183) seroconverted two weeks post second immunization, irrespective of age (18–75); 56% (102/183) had detectable neutralising antibodies. Almost all (28/31) SARS-CoV-2 positive individuals had increased S IgG binding antibodies following their first 1.0 μg dose with a ≥0.5log10 increase in 71% (22/31). Interpretation: encapsulated saRNA was well tolerated and immunogenic in adults aged 18–75 years. Seroconversion rates in antigen naïve were higher than those reported in our dose-ranging study. Further work is required to determine if this difference is related to a longer dosing interval (14 vs. 4 weeks) or dosing with 1.0 μg followed by 10.0 μg. Boosting of S IgG antibodies was observed with a single 1.0 μg injection in those with pre-existing immune responses.Funding: grants and gifts from the Medical Research Council UKRI (MC_PC_19076), the National Institute for Health Research/Vaccine Task Force, Partners of Citadel and Citadel Securities, Sir Joseph Hotung Charitable Settlement, Jon Moulton Charity Trust, Pierre Andurand, and Restore the Earth.

Recurrent disease and treatment-associated chemoresistance are the two main factors accounting for poor clinical outcomes of ovarian cancer (OC) patients. Both can be associated with cancer stem cells (CSCs), which contribute to cancer formation, progression, chemoresistance, and recurrence. Hence, this study investigated whether the expression of known CSC-associated markers ALDH1A, CD44, and CD133 may predict OC patient prognosis. We analyzed their expression in primary epithelial ovarian cancer (EOC) patients using immunohistochemistry and related them to clinicopathological data, including overall survival (OS) and progression-free survival (PFS). Expression of ALDH1A1 was detected in 32%, CD133 in 28%, and CD44 in 33% of cases. While Kaplan–Meier analysis revealed no association of the expression of CD133 and CD44 with PFS and OS, ALDH1A1-positive patients were characterized with both significantly shorter OS (p = 0.00022) and PFS (p = 0.027). Multivariate analysis demonstrated that the expression of ALDH1A1, FIGO stage III–IV, and residual disease after suboptimal debulking or neoadjuvant chemotherapy correlated with shorter OS. The results of this study identify ALDH1A1 as a potential independent prognostic factor of shorter OS and PFS in EOC patients. Therefore, targeting ALDH1A1-positive cancer cells may be a promising therapeutic strategy to influence the disease course and treatment response.

The aim of this study was to investigate the relationship between lactoferrin and iron and its binding proteins in women with endometriosis by simultaneously measuring these parameters in plasma and peritoneal fluid. Ninety women were evaluated, of whom 57 were confirmed as having endometriosis. Lactoferrin was measured by ELISA, transferrin, ferritin and iron on a Cobas 8000 analyser. Lactoferrin and transferrin in peritoneal fluid were lower compared to plasma, in contrast to ferritin and iron. In plasma, lactoferrin showeds associations with iron and transferrin in endometriosis and with ferritin in the group without endometriosis. Lactoferrin in peritoneal fluid correlated with lactoferrin, iron and transferrin of plasma in patients without endometriosis. The ratio of lactoferrin concentration in peritoneal fluid to plasma differentiated stage I versus IV of endometriosis and was negatively correlated with the iron ratio in patients without endometriosis. The ferritin ratio differentiated women with and without endometriosis. The very high ferritin ratios, especially in advanced stages of endometriosis, suggest the protective involvement of this protein in peritoneal fluid and the loss of this role by lactoferrin. The results demonstrate the validity of assessing iron metabolism in women with endometriosis, which may be useful as a marker of the disease and its progression.

Chest X-rays (CXRs) are the first-line investigation in patients presenting to emergency departments (EDs) with dyspnoea and are a valuable adjunct to clinical management of COVID-19 associated lung disease. Artificial intelligence (AI) has the potential to facilitate rapid triage of CXRs for further patient testing and/or isolation. In this work we develop an AI algorithm, CovIx, to differentiate normal, abnormal, non-COVID-19 pneumonia, and COVID-19 CXRs using a multicentre cohort of 293,143 CXRs. The algorithm is prospectively validated in 3289 CXRs acquired from patients presenting to ED with symptoms of COVID-19 across four sites in NHS Greater Glasgow and Clyde. CovIx achieves area under receiver operating characteristic curve for COVID-19 of 0.86, with sensitivity and F1-score up to 0.83 and 0.71 respectively, and performs on-par with four board-certified radiologists. AI-based algorithms can identify CXRs with COVID-19 associated pneumonia, as well as distinguish non-COVID pneumonias in symptomatic patients presenting to ED. Pre-trained models and inference scripts are freely available at https://github.com/beringresearch/bravecx-covid.