1. Differential gene expression profiles of normal human parotid and submandibular glands
- Author
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Sun Qh, Jie Du, Sun Qf, and Songlin Wang
- Subjects
medicine.medical_specialty ,Saliva ,Submandibular Gland ,KCNJ15 ,Cystic Fibrosis Transmembrane Conductance Regulator ,Organic Anion Transporters ,Wnt-5a Protein ,stomatognathic system ,Proto-Oncogene Proteins ,Internal medicine ,Complementary DNA ,Gene expression ,medicine ,Humans ,Parotid Gland ,Glucuronosyltransferase ,Potassium Channels, Inwardly Rectifying ,General Dentistry ,Gene ,Oligonucleotide Array Sequence Analysis ,Symporters ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,Mucins ,Reverse Transcription ,Submandibular gland ,Drug Resistance, Multiple ,Up-Regulation ,Parotid gland ,Wnt Proteins ,Gene expression profiling ,stomatognathic diseases ,medicine.anatomical_structure ,Endocrinology ,Otorhinolaryngology ,biology.protein ,Cytokines ,N-Acetylgalactosaminyltransferases ,RNA ,Multidrug Resistance-Associated Proteins ,Carrier Proteins - Abstract
Background: Parotid and submandibular glands have different properties including characteristics of the secreted saliva and tumor incidences. The differences in properties of parotid and submandibular glands are not clear from a genetic viewpoint. Objective: To study differential gene expression profiles between normal human parotid and submandibular glands. Materials and methods: Three pairs of normal parotid and submandibular glands were obtained. RNA was extracted from these samples. After reverse transcription, the cDNA was in vitro-transcribed to produce biotin-labeled cRNA. The purified biotin-labeled cRNA samples were hybridized to microarray chips. Results: Among the 54 675 tested transcripts, 47 transcripts were upregulated at least twofold in the parotid gland compared with the submandibular gland, including tumor-associated genes (pleiotrophin, WNT5A, ABCC1) and transport-associated genes (SLCO1A2, SLC13A5, KCNJ15). Ninety-eight transcripts were upregulated at least twofold in the submandibular gland compared with the parotid gland, including the chloride channel CFTR and mucin-associated genes that belong to the starch and sucrose metabolism pathway (GalNAc-T4, GalNAc-T7 and GalNAc-T13). Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of nine differentially expressed genes confirmed the microarray results. Conclusion: This study revealed the different gene expression profiles of normal human parotid and submandibular glands, providing a genetic basis for their differing properties.
- Published
- 2008