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Supplement Figure 1 PCA (A) indicates clear segregation of cells before and after differentiation. Cells after 35-day and 70-day differentiation create groups very distinct from hiPSCs. It is also noticeable that cells at both differentiation time points share 30.7% up- and 27.5% down-regulated genes (B). Supplement Figure 2 35 days after differentiation, 198 genes were significantly down-regulated and 99 were up-regulated in the treated group versus the hiPSC cells (A). The second stage of differentiation (Day 70 vs. Day 35) resulted in barely noticeable gene expression changes: 0 genes were downregulated and 8 genes were upregulated (B). Supplement Figure 3 The connection between genes and GO terms with visualization of logFC values and gene symbols was demonstrated. To better show all dependencies those results were compiled with a heatmap involving gene expression of all compared groups (Day 70 vs. hiPSCs; Day 35 vs. hiPSCS and Day 70 vs. Day 35). Supplement Figure 4 GATA4 (FC=1.98, p≤0.05) as an endodermal marker (i), α-smooth α-SMA (FC=0.28, P≤0.001) and Brachyury as mesodermal markers (ii), Vimentin and PAX6 (FC=0.872, P>0,05) as ectodermal markers (iii), as well as CD133 (FC=0.61, P≤0.05), CD117 (FC=0.989, P>0.05), CD44 (FC=0.46, P≤0.01), SOX2 (FC=0.639, P>0.05) as stem and/or cancer cell markers (iv) after 70-day differentiation. Supplement Figure 5 The most upregulated genes CGA and IRS4 were subjected to further qPCR evaluation in both hiPSCs cell lines separately (A, B). The differentiated hiPS ND41658*H cells demonstrated elevated gene expression as follows: CGA (FC=573.6, P>0.05) and IRS4 (FC=255.5, P≤0.01). HiPS GPCCi001-A cells after 70-day differentiation were characterized by increased gene expression levels in the following manner: CGA (FC=564, P≤0.05) and IRS4 (FC= 220.2, P≤0.05). Based on that, we confirmed the expression of CGA and PPDPFL at the protein level by immunohistochemistry in hiPSC GPCCi001-A cell line (C).