5 results on '"Sokolowski, Kamil A."'
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2. Additional file 2 of Targeted beta therapy of prostate cancer with 177Lu-labelled Miltuximab® antibody against glypican-1 (GPC-1)
- Author
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Yeh, Mei-Chun, Tse, Brian W. C., Fletcher, Nicholas L., Houston, Zachary H., Lund, Maria, Volpert, Marianna, Stewart, Chelsea, Sokolowski, Kamil A., Varinder Jeet, Thurecht, Kristofer J., Campbell, Douglas H., Walsh, Bradley J., Nelson, Colleen C., and Russell, Pamela J.
- Abstract
Additional file 2: Fig. S1. a Radio-TLC of purified antibody conjugates showing successful radiolabelling of DFO-Miltuximab® with 89Zirconium. b Radio-TLC of purified antibody conjugates showing successful radiolabelling of DOTA-Miltuximab® with 177Lutetium. Fig. S2. Immunoreactivity of a,c DFO-Miltuximab® and b,d DOTAMiltuximab® to cell surface GPC-1 on DU-145 cells measured via flow cytometry (a,b) and ELISA (c,d). For some tests, antibody was “mock” radiolabelled, i.e. treated in the same way as the radiolabelled antibody, but without the addition of radiolabel, to estimate the effect of radiolabelling conditions on immunoreactivity. Fig. S3. Quantitative flow cytometry analysis of DU-145 cells using MIL-38 binding for quantification. MIL-38 was used with the QIFIKIT antigen density analysis kit to determine GPC-1 density on the cell surface of a. prostate cancer cell line DU-145 and b. GPC1 negative lymphoma cell line Raji. Fig. S4. No effect of DOTA-Miltuximab® alone on in vivo tumour growth. DU-145-RFP-Luc cells (5x106 ) in matrigel were injected subcutaneously into the right flank of BALB/c/nude mice. When tumours reached ~100mm3 , mice were injected with saline (n=6) or DOTA-Miltuximab® (n=6) (80ug) intravenously. All mice were euthanised approximately 2 weeks thereafter. a Mean weekly mouse tumour volume. d Individual mouse tumour volume at endpoint. Data expressed as Mean ± SEM and statistical analysis performed using an unpaired t test. *p < 0.05. Fig. S5. Representative H&E staining of the mouse brain, heart, lung, liver, kidney, spleen, small intestine and testis tissue a 3 days, b 5 days, c 7 days and d 27 days post 6MBq [ 177Lu]Lu-DOTA-Miltuximab® treatment or e 27 days post DOTA-Miltuximab® treatment. Fig. S6. Individual weekly mouse tumour volumes of mice treated with a DOTAMiltuximab® (n=8) b 3MBq [ 177Lu]Lu-DOTA-Miltuximab® (n=9) or c 10MBq [ 177Lu]LuDOTA-Miltuximab® (n=9) treated DU-145 xenograft mice.
- Published
- 2020
- Full Text
- View/download PDF
3. Additional file 1 of Targeted beta therapy of prostate cancer with 177Lu-labelled Miltuximab® antibody against glypican-1 (GPC-1)
- Author
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Yeh, Mei-Chun, Tse, Brian W. C., Fletcher, Nicholas L., Houston, Zachary H., Lund, Maria, Volpert, Marianna, Stewart, Chelsea, Sokolowski, Kamil A., Varinder Jeet, Thurecht, Kristofer J., Campbell, Douglas H., Walsh, Bradley J., Nelson, Colleen C., and Russell, Pamela J.
- Abstract
Additional file 1. Supplementary Methods.
- Published
- 2020
- Full Text
- View/download PDF
4. Plasmon-induced optical control over dithionite-mediated chemical redox reactions
- Author
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Huang, Junyang, De Nijs, Bart, Cormier, Sean, Sokolowski, Kamil, Grys, David-Benjamin, Readman, Charlie A, Barrow, Steven J, Scherman, Oren A, and Baumberg, Jeremy J
- Subjects
0306 Physical Chemistry (incl. Structural) ,FOS: Nanotechnology ,0302 Inorganic Chemistry ,Nanotechnology ,Bioengineering ,0303 Macromolecular and Materials Chemistry ,7. Clean energy ,0305 Organic Chemistry - Abstract
External-stimuli controlled reversible formation of radical species is of great interest for synthetic and supramolecular chemistry, molecular machinery, as well as emerging technologies ranging from (photo)catalysis and photovoltaics to nanomedicine. Here we show a novel hybrid colloidal system for light-driven reversible reduction of chemical species that, on their own, do not respond to light. This is achieved by the unique combination of photo-sensitive plasmonic aggregates and temperature-responsive inorganic species generating radicals that can be finally accepted and stabilised by non-photo-responsive organic molecules. In this system Au nanoparticles (NPs) self-assembled via sub-nm precise molecular spacers (cucurbit[n]urils) interact strongly with visible light to locally accelerate the decomposition of dithionite species (S2O42-) close to the NP interfaces. This light-driven process leads to the generation of inorganic radicals whose electrons can then be reversibly picked up by small organic acceptors, such as the methyl viologen molecules (MV2+) used here. During light-triggered plasmon- and heat-assisted generation of radicals, the S2O42- species work as a chemical 'fuel' linking photo-induced processes at the NP interfaces with redox chemistry in the surrounding water environment. By incorporating MV2+ as a Raman-active reporter molecule, the resulting optically-controlled redox processes can be followed in real-time.
5. Plasmon-induced optical control over dithionite-mediated chemical redox reactions
- Author
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Junyang Huang, Jeremy J. Baumberg, Charlie Readman, David-Benjamin Grys, Kamil Sokołowski, Steven J. Barrow, Oren A. Scherman, Bart de Nijs, Sean Cormier, Huang, Junyang [0000-0001-6676-495X], de Nijs, Bart [0000-0002-8234-723X], Cormier, Sean [0000-0003-2973-8722], Sokolowski, Kamil [0000-0002-2481-336X], Grys, David-Benjamin [0000-0002-4038-6388], Readman, Charlie A [0000-0001-9743-9180], Barrow, Steven J [0000-0001-6417-1800], Scherman, Oren A [0000-0001-8032-7166], Baumberg, Jeremy J [0000-0002-9606-9488], and Apollo - University of Cambridge Repository
- Subjects
Radical ,Supramolecular chemistry ,Nanoparticle ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,Photochemistry ,Dithionite ,0305 Organic Chemistry ,7. Clean energy ,01 natural sciences ,Redox ,chemistry.chemical_compound ,0302 Inorganic Chemistry ,Water environment ,Nanotechnology ,Molecule ,Physical and Theoretical Chemistry ,0306 Physical Chemistry (incl. Structural) ,Chemistry ,0303 Macromolecular and Materials Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Chemical species ,0210 nano-technology - Abstract
External-stimuli controlled reversible formation of radical species is of great interest for synthetic and supramolecular chemistry, molecular machinery, as well as emerging technologies ranging from (photo)catalysis and photovoltaics to nanomedicine. Here we show a novel hybrid colloidal system for light-driven reversible reduction of chemical species that, on their own, do not respond to light. This is achieved by the unique combination of photo-sensitive plasmonic aggregates and temperature-responsive inorganic species generating radicals that can be finally accepted and stabilised by non-photo-responsive organic molecules. In this system Au nanoparticles (NPs) self-assembled via sub-nm precise molecular spacers (cucurbit[n]urils) interact strongly with visible light to locally accelerate the decomposition of dithionite species (S(2)O(4)(2–)) close to the NP interfaces. This light-driven process leads to the generation of inorganic radicals whose electrons can then be reversibly picked up by small organic acceptors, such as the methyl viologen molecules (MV(2+)) used here. During light-triggered plasmon- and heat-assisted generation of radicals, the S(2)O(4)(2–) species work as a chemical ‘fuel’ linking photo-induced processes at the NP interfaces with redox chemistry in the surrounding water environment. By incorporating MV(2+) as a Raman-active reporter molecule, the resulting optically-controlled redox processes can be followed in real-time.
- Published
- 2019
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