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BACKGROUND: Sustainable employability is increasingly important with current socio-economic challenges. Screening for resilience could contribute to early detection of either a risk, or a protector for sustainable employability, the latter being operationalized as workability and vitality. OBJECTIVE: To study the predictive value of Heart Rate Variability (HRV) measurements and the Brief Resilience Scale (BRS) for worker self-reported workability and vitality after 2–4 years. METHODS: Prospective observational cohort study with mean follow-up period of 38 months. 1,624 workers (18–65 years old) in moderate and large companies participated. Resilience was measured by HRV (one-minute paced deep breathing protocol) and the BRS at baseline. Workability Index (WAI), and the Vitality dimension of the Utrecht Work Engagement Scale-9 (UWES-9-vitality) were the outcome measures. Backward stepwise multiple regression analysis (p

The European Commission proposed a Directive on Platform Work at the end of 2021. While much attention has been placed on its effort to address misclassification of the employed as self-employed, it also contains ambitious provisions for the regulation of the algorithmic management prevalent on these platforms. Overall, these provisions are well-drafted, yet they require extra scrutiny in light of the fierce lobbying and resistance they will likely encounter in the legislative process, in implementation and in enforcement. In this article, we place the proposal in its sociotechnical context, drawing upon wide cross-disciplinary scholarship to identify a range of tensions, potential misinterpretations, and perversions that should be pre-empted and guarded against at the earliest possible stage. These include improvements to ex ante and ex post algorithmic transparency; identifying and strengthening the standard against which human reviewers of algorithmic decisions review; anticipating challenges of representation and organising in complex platform contexts; creating realistic ambitions for digital worker communication channels; and accountably monitoring and evaluating impacts on workers while limiting data collection. We encourage legislators and regulators at both European and national levels to act to fortify these provisions in the negotiation of the Directive, its potential transposition, and in its enforcement.

This special issue of the European Labour Law Journal, edited by Jeremias Adams-Prassl, Halefom Abraha, Aislinn Kelly-Lyth, Sangh Rakshita and Michael ‘Six’ Silberman, explores the regulation of Algorithmic Management in the European Union and beyond. In our guest editorial, we set out the background to the project, introduce the reader to the key themes and highlights of the papers to follow, and acknowledge the support that the project has enjoyed.

PURPOSE: We aimed to understand how new diagnosis discussions are conducted in pediatric oncology, and the training provided for their conduct. METHODS: This mixed-methods study used a sequential exploratory design. Qualitative interviews (n = 20) were conducted with pediatric oncologists (n = 15) and fellows (n = 5) at a single institution, focusing on the process used to convey the diagnosis and treatment plan to the family. Accreditation Council for Graduate Medical Education–accredited pediatric oncology fellowship program directors (n = 38) and fellows (n = 70) were subsequently surveyed to confirm qualitative results and elucidate the training that fellows receive in conducting new diagnosis discussions. RESULTS: Our findings suggest that new diagnosis discussions in pediatric oncology are typically conducted in three stages: (1) concern for cancer; (2) confirmation of diagnosis; and (3) treatment plan/consent, and are fundamentally similar across settings; however, pediatric oncologists skillfully tailor their approach on the basis of clinical circumstances and parental needs. Decisions regarding inclusion of the child are primarily determined by parental preference, whereas inclusion of health care team members is driven by physician role (ie, trainee v program director) and health care organization–related factors. Physician preparation for discussions involves logistical, intellectual, and emotional components. Disclosure of prognosis is nuanced. There is variability across pediatric oncology fellowship programs in the provision of training for these discussions. CONCLUSION: We identified common practices of pediatric oncologists as they prepare for and lead new diagnosis discussions in pediatric oncology. We found variability in the training that pediatric oncology fellows receive regarding how to conduct these discussions, highlighting a need for standardized training curricula.

In an interview in the early 1980s, Michel Foucault predicated that, from the eighteenth century on, modern government rationality has essentially been a form of urban planning. This article challenges this argument. It discusses the formation of rural social engineering, that is, the state-led efforts to design new men and new social orders outside the cities through plans, during decolonisation in Asia. Based on the comparative study of India after Partition (1947) and British Malaya during the counter-insurgency war (1948–60), the argument is that we can understand rural social engineering particularly in the 1950s less as a consequence of colonial inheritance or international change but as the result of how decolonisation unfolded including its patterns of violence, social conflict, and migration. As such, rural social engineering constituted a central element in the postcolonial ‘art of the government of man’.

We investigate the default probability of Indonesian banks using the copula approach and analyze the macro-financial factors that drive them. We use quarterly data comprised of 80 banks from 2005 to 2019. We find empirical evidence that Common Equity Tier 1 (CET 1) ratio, inefficiency ratio, and deposit ratio have negativelyimpacted the bank’s default probability. We also find that macroeconomic variables such as policy rate, real exchange, economic growth, and unemployment reduce the default probability. Our study suggests that regulators should focus on capital and deposit management policies to reduce bank risk-taking behaviour. Additionally, the policy rate effectively anticipated the banks’ default risk.

In this article, we review the distrust literature and explore whether the universal sequence for trust as outlined by Dietz (2011) and Dietz and Den Hartog (2006) is also true for distrust. Compared to trust, there is much less research on distrust, although the field has been rapidly developing in recent years. We argue that it is time to explore a universal sequence for distrust to take stock of current knowledge and to focus the empirical and conceptual research. There is sufficient evidence to suggest that such a universal sequence is a valuable framework for distrust research. This analytical exercise also forces us to identify tacit assumptions that frame and guide much of the current distrust (and trust) research. In so doing, we identify two main areas that require more attention: 1) the definition of distrust and its relation to trust and 2) the universal sequences and their dynamics. These findings lead to avenues for further research.

We present an approach for implementing a formally certified loop-invariant code motion optimization by composing an unrolling pass and a formally certified yet efficient global subexpression elimination. This approach is lightweight: each pass comes with a simple and independent proof of correctness. Experiments show the approach significantly narrows the performance gap between the CompCert certified compiler and state-of-the-art optimizing compilers. Our static analysis employs an efficient yet verified hashed set structure, resulting in the fast compilation.

Intensified human activities can augment soil organic carbon (SOC) losses from the world’s croplands, making SOC a highly dynamic parameter both in space and time. Sentinel-2 spectral imagery is well placed to capture the spatiotemporal variability of SOC, but its capability has only been demonstrated for agricultural regions mostly located in Europe. Furthermore, most studies so far only used single-date images that resulted in spatially non-continuous SOC maps, hampering their ability to aid multiscale SOC assessments. Here, we aim to achieve spatially continuous mapping of SOC in croplands, by creating multitemporal bare soil composites that maximize cropland coverage in two regions of varying agroecosystems and landscape structure in the Northeast China Chernozem region and the Belgian Loam Belt. Bare soil pixels were extracted via spectral index thresholding that excluded contaminated pixels from external perturbance. Multitemporal soil composites were then obtained by averaging over multiple single-date bare soil images that were selected within pre-determined optimal time-windows, corresponding to the region-specific crop sowing periods when best possible surface conditions were expected. Results show that the optimal time-window filter ensured selective inclusion of single-date images that themselves yielded stable and robust SOC predictions across multiple years. Spectral-based models developed from multitemporal composites consistently produced better or similar prediction accuracies than single-date images for both study regions (R2: 0.52–0.62; RMSE: 0.17–0.21 g 100 g−1), while also achieved maximum cropland coverage (>82 %). Bootstrap modelling demonstrated that SOC mapping via multitemporal Sentinel-2 data was associated with small uncertainties. Investigations into the significant spectral bands that contributed to the prediction of SOC suggested that, regardless of the study regions, the physical relationship between spectral bands and SOC that predominantly exists for laboratory spectra is largely translated into Sentinel-2 platforms. This study highlights the widespread applicability of multitemporal Sentinel-2 remote sensing for effective and high-resolution SOC mapping, in order to detect localized soil degradation as well as to inform regional cropland management in diverse agroecosystems.

Mental health apps have shown promise in improving quality of life in a variety of contexts; however, it is unclear how specific internal app features influence the effectiveness of such apps. The purpose of this study is to investigate the effect of app feature personalization, including avatar customization and naming, on depressive symptoms. A total of 59 students completed a 14-day trial utilizing a novel cognitive behavioral therapy-based app developed exclusively for this study. The study entailed a two-group experimental design with randomization to either a personalization or no-personalization version of the app. AirHeart successfully reduced depressive symptoms over the two-week period, but personalization of an avatar did not further this reduction.

Soil health laws should account for global soil connections

Ecological intensification (EI) could help return agriculture into a ‘safe operating space’ for humanity. Using a novel application of meta-analysis to data from 30 long-term experiments from Europe and Africa (comprising 25,565 yield records), we investigated how field-scale EI practices interact with each other, and with N fertilizer and tillage, in their effects on long-term crop yields. Here we confirmed that EI practices (specifically, increasing crop diversity and adding fertility crops and organic matter) have generally positive effects on the yield of staple crops. However, we show that EI practices have a largely substitutive interaction with N fertilizer, so that EI practices substantially increase yield at low N fertilizer doses but have minimal or no effect on yield at high N fertilizer doses. EI practices had comparable effects across different tillage intensities, and reducing tillage did not strongly affect yields.

Upon infection, B lymphocytes develop clonal responses. In teleost fish, which lack lymph nodes, the kinetics and location of B cell responses remain poorly characterized. Fish pronephros is the site of B cell differentiation and the main niche for persistence of plasma cells. In this study, we undertook the analysis of the rainbow trout IgHμ repertoire in this critical tissue for humoral adaptive immunity after primary immunization and boost with a rhabdovirus, the viral hemorrhagic septicemia virus (VHSV). We used a barcoded 5′ RACE-cDNA sequencing approach to characterize modifications of the IgHμ repertoire, including VH usage in expressed V(D)J rearrangements, clonal diversity, and clonotype sharing between individual fish and treatments. In the pronephros, our approach quantified the clonotype frequency across the whole IgH repertoire (i.e., with all VH), measuring the frequency of Ag-responding clonotypes. Viral infection led to extensive modifications of the pronephros B cell repertoire, implicating several VH subgroups after primary infection. In contrast, only modest changes in repertoire persisted 5 mo later, including VHSV-specific public expansions. The IgM public response implicating IgHV1-18 and JH5, previously described in spleen, was confirmed in pronephros in all infected fish, strongly correlated to the response. However, the distribution of top clonotypes showed that pronephros and spleen B cells constitute distinct compartments with different IgH repertoires. Unexpectedly, after boost, the frequency of anti-VHSV clonotypes decreased both in pronephros and spleen, raising questions about B cell circulation. A better monitoring of B cell response kinetics in lymphoid tissues will be an essential step to understand B memory and plasmocyte formation mechanisms in fish.

I seek to emphasize Immanuel Kant’s lingering and unsavory impact on medical ethics by emphasizing Kantian ethics’ disregard for non- rational humans. We must be considerate when discussing individuals who have some form of dementia, conditions that irreversibly diminish the ability to use rational thought, sometimes to a degree of severity that hinders essential daily functions. I argue that to consider ourselves proponents of human equality we must treat humans with dementia as members of the kingdom of ends.

With climate change and an increasing global human population, the concept of agroforestry is gaining economic and environmental interest. The practice of growing trees and crops on the same land is mainly applied in (sub)tropical climate and rarer in temperate areas where farmers fear decreased understorey crop yields due to competition with trees. However, whether competition is stronger below- (soil moisture, nutrients) or aboveground (light) in a temperate silvoarable agroforestry system (AFS) is not clear. The effects of different treatments of light, water and nutrient availability on crop production in two temperate AFS in Central Switzerland were investigated, where summer barley (Hordeum vulgare L.) was grown as understorey crop under 90%, 40% and 0% shade nets, with and without irrigation and/or fertilisation in a fully factorial design. Yield was reduced by 26% under heavy shade; yield reductions under moderate shade were not significant. Fertilisation and irrigation increased crop yield by 13% and 6-9%, respectively, independent from shade. Individual seed mass was significantly increased by fertilisation from an average of 0.041 g (+/- 0.008 SD) in unfertilised treatments to an average of 0.048 g (+/- 0.010) in fertilised treatments. Fertilisation had the biggest impact on total seed number (p < 0.001) with on average 36 (+/- 26) seeds per individual in unfertilised plots and 61 (+/- 33) in fertilised plots. This study demonstrates that moderate shade (as can be expected in modern AFS) was not a major limiting factor for barley yield in these two AFS in Switzerland, indicating that AFS with appropriate management combined with suitable selection of understorey crops are an option for agricultural production in temperate regions without significant yield losses., Agroforestry Systems, 96 (4), ISSN:0167-4366, ISSN:1572-9680

In this work instabilities in fixed bed reactors with downwards directed flows for the oligomerization of 1-butene were investigated. For very long residence times or very low velocities in combination with strong exothermic reactions, the buoyancy force can exceed the inertia force and the downwards directed liquid flow in the fixed bed becomes instable. These flow conditions might result in hotspots, the shift of the conversion towards unwanted by-products or even in a runaway of the reactor. Therefore, the detailed understanding of the transition between stable and instable flow conditions in fixed bed reactors is essential for a safe and reliable operation of the reactor. Various simulation methods and correlations were applied to predict instable conditions that were observed in an experimental setup. 3D CFD simulations could be used to predict instable flow conditions in fixed bed reactors.

In the light of the Second World War, the devastation of Hiroshima and Nagasaki, and decolonization in Asia, the newly established UN organization for education, science and culture (UNESCO) initiated a global research project in 1947. Its main task was to find out how tensions within and between societies can be explained and tackled to secure peace and social justice. Combining peace history with the global history of social sciences and global intellectual history, this article assesses the design and conduct of the Tensions Project in India and Israel at the crossroads of post-fascism and post-colonialism. It finds the reasons for the Project's limited impact in the contradictions between universal knowledge claims and local specificities, its over-confidence in scientific solutions for social problems, interfering nation-building in early postcolonial states, the limited comparability of research, and its neglect of political activism.

Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Fonds pour l'innovation du système de santé (FISS) - France. Background Cardiac rehabilitation (CR) has demonstrated scientific benefits in terms of morbidity and mortality. In France, 2019 national data indicates that only 22,3% of coronary patients benefit from CR. The development of alternative programs to center-based cardiac rehabilitation (CBCR) must be considered as a public health priority and requires a reorganization of ambulatory care pathways. Purpose The objective of the "Walk Hop" program is to demonstrate that home-based cardiac tele-rehabilitation (HBCT) is a complementary and safe approach to CBCR, able to increase the proportion of patients benefiting from a rehabilitation program. Methods This feasibility study started begin of 2022. 3,200 coronary patients will be recruited in 8 participating cardiac rehabilitation centers (CRC). Total duration of the experiment is 42 months. The program is financially supported by the French authorities. The HBCT solution developed for the study is proposed to patients by the CRC cardiologist. A multidisciplinary platform consisting of a secure portal and an application allows bidirectional communication. Patients are equiped with a cycle ergometer and a validated heart rate monitor. The application presents aerobic and muscle strenghtening activities and therapeutic education and collects data from the sessions carried out, adherence to the program, symptoms and quality of life. Results By october 2022, 157 active patients have been included in the program with a large majority of men (86%). Mean age is 53,9 ± 9,0. Hypercholesterolemia is noted in 36,9% of the included patients, smoking in 31%, arterial hypertension in 30,6% and diabetes in 11,1%. Obesity/overweight affects 60% of the population. Mean time from angioplasty to initiation of the rehabilitation is 81 ± 90 days. Mean number of home rehabilitation sessions is 21 and a total of 3342 home sessions have been realized. No cardiovascular event or rehospitalization have been registered since the beginnng. Mean adherence to the prescribed sessions is 80 ± 20% and the mean power gain at the end of the program is 14,6 ± 9,9 %. 94% of the patients expressed satisfaction or high satisfaction at the end of the program. Conclusion(s) The Walk Hop program is evaluating the feasibility and acceptability of HBCT with remote monitoring and digital assistance by smartphone. The preliminary data are encouraging in view of the final expected impact: greater cost-effective access to rehabilitation programs by removing obstacles like distance or resumption of work. It is also expected that HBCT will provide an improvement in the long-term maintenance benefits.

Background Wildfire is a landscape disturbance important for stream ecosystems and the recruitment of large wood (LW; LW describes wood in streams) into streams, with post-fire management also playing a role. We used a stratified random sample of 4th-order watersheds that represent a range of pre-fire stand age and fire severity from unburned to entirely burned watersheds to 1) determine whether watershed stand age (pre-fire) or fire severity affected riparian overstory survival, riparian coarse wood (CW; CW describes wood in riparian areas), LW, or in-stream physical, chemical, and biological responses; and 2) identify relationships of LW with riparian vegetation and in-stream physical, chemical, and biological factors. Results At higher fire severities, LW and CW diameter was smaller, but volume did not change in the first year post-fire. Larger size of CW in riparian areas versus LW in streams suggests potential future recruitment of larger-diameter wood into streams from riparian zones in severely burned watersheds. Fire severity exerted strong control on stream responses across watersheds, explaining more of the variation than stand age. At higher fire severities, riparian tree mortality, salvage logging, light, and dissolved organic matter (DOM) concentrations were higher, whereas canopy cover, LW diameter, macroinvertebrate diversity, and fish density were lower. In watersheds with older stand ages, elevation and mean annual precipitation were greater but mean annual temperature, specific ultra-violet absorption at 254 nm, and phosphorus concentrations were lower. Overstory mortality in burned riparian areas was lower for red alder (12%) than western redcedar (69%). Conclusions Our results link forested streams, fire, and LW by identifying key relationships that change with fire severity and/or watershed stand age. Severe fires burn more overstory riparian vegetation, leading to increased light, DOM concentrations, and macroinvertebrate densities, along with reduced canopy cover, LW diameter, macroinvertebrate diversity, and fish densities. We highlight an important function of red alder in riparian zones—as a fire-resistant species, it may help facilitate a more rapid recovery for streams in fire-prone landscapes. Continued comprehensive aquatic and riparian ecosystem monitoring of these watersheds will aid in understanding long-term effects of post-fire management activities (salvage logging) on aquatic ecosystems.

Sustainable intensification practices, such as integrated soil fertility management (ISFM), form a strategy to close yield gaps while maintaining soil fertility and, typically, are locally tested in field trials. However, to estimate the potential impact of ISFM on a regional scale, field trials are insufficient and biogeochemical models are required. These models need to be calibrated and evaluated when applied to new environments. Here, we present a robust calibration of the DayCent agroecosystem model to simulate the impact of ISFM practices on maize productivity in Kenya, using a probabilistic Bayesian calibration technique with data from long-term field trials at four sites in central and western Kenya. We assessed the efficiency of DayCent in simulating: 1) maize grain yield, 2) changes in soil organic carbon (SOC), and 3) nutrient use efficiency of applied nitrogen (N) fertilizer under different ISFM treatments, which consisted of different organic resources combined with the addition or absence of mineral N fertilizer. After model calibration, both the simulations of maize yield (Nash Sutcliffe Efficiency, NSE 0.51) and change in SOC (NSE 0.54) improved significantly compared to runs using the standard DayCent parameters (NSE of 0.33 and -1.3 for yield and SOC change, respectively). A leave-one-site-out cross evaluation indicated the robustness of the approach for spatial extrapolation, i.e., the significant improvement of model simulations was achieved by calibrating the model with data from three sites and then evaluating it with data from the remaining site. The values of model parameters related to SOC decomposition were most altered by the calibration, i.e., they were an order of magnitude higher compared to the default parameter values (derived for temperate climates). This suggests that the DayCent temperature function is not suitable to capture SOC decomposition across climates with a single set of parameter values. Further, similar maize yields were simulated for all treatments that received mineral N fertilizer and DayCent underestimated the yield increase observed in the field trials of the combined application of organic resources and mineral N compared sole mineral N application. In contrast, at low levels of nutrient inputs DayCent proved sufficiently sensitive to capture differences in maize yield levels. Finally, while mean yields by treatment were simulated well, year-to-year yield variation was not captured well by DayCent. In summary, our results indicate that DayCent is capable to estimate the mean impact that ISFM practices at typical rates of mineral fertilizer and organic resource applications have on yield and SOC, but may not be capable to estimate the differences in yield potential at very high inputs. While the cross evaluation indicated a robustness for upscaling, the suboptimal representation of year-to-year yield variabilities shows that future projections under a changing climate may be biased by the DayCent model. Consequently, improved model structures, such as improved soil moisture representation, are needed to reduce uncertainty.

In this study, the spatio-temporal changes in Rainwater Productivity (RP) and its sensitivity to the changes in precipitation and temperature predicted by climate models in various climatic zones across the rainfed agricultural areas of Ethiopia were analyzed. First, the future precipitation, air temperature, and shortwave radiation from multiple GCM projections were downscaled to a 0.05°x0.05° grid resolution, considering three shared socioeconomic pathways (SSP1-2.6, SSP2-4.5, and SSP5-8.5) and three future periods: 2020-2049, 2045-2074, and 2070-2099 using the present climate (1981-2010) as a reference. Next, the reference potential evapotranspiration was computed using the FAO Penman-Monteith and the actual evapotranspiration was simulated using a daily soil water balance model. Then, the relative crop yield (i.e., the ratio of the actual and water-limited potential yield) was determined as a function of the evaporative stress index and crop yield response factor (Ky) for the two growing seasons -- the main (meher) growing season (May-Sep) and the shorter (belg) growing season (Feb-May) for the present and future climates. The computed relative yield was used as a proxy for RP, under the assumption that effective rainfall is the limiting factor for crop yield. Finally, the sensitivity of RP to projected changes in precipitation and temperature was analyzed based on the one-at-a-time (OAT) approach for warmer and drier versus warmer and wetter climate scenarios.The results show that under the present climate, the median RP (percent of the potential RP) during the Meher and Belg seasons ranges from about 52% and 34% in semi-arid climates to 93% and 45% in humid climates. The projected Meher RP in the future shows either a slight change or a decrease by up to 10% across the majority of the RFA regions under all SSPs and future periods. Conversely, the Belg season RP is likely to increase by up to 15% across the major Belg-producing regions by the end of the century. The observed changes are the combined effects of the nearly consistent but spatially variable increase in precipitation (for example up to 30% under SSP5-8.5 in the 2080s) and rising temperature (up to 5°C under SSP5-8.5 in the 2080s) over the RFA region. The OAT sensitivity analysis reveals that RP under warmer and drier climates is strongly sensitive to precipitation. However, under warmer and wetter conditions the climate sensitivity of RP is determined by the rainfall regime, i.e, in the areas with unimodal rainfall regimes, changes in RP are dominated by the changes in precipitation while in areas with strongly erratic or bimodal rainfall distribution, temperature, or both precipitation and temperature control the changes in RP. Such analyses are useful for assessing the future climate risks to crop yield due to water stress associated with the expected increases in atmospheric evaporative demand, identifying vulnerable areas across the RFA region as well as possibilities for agricultural expansion.

Climate change is causing negative effects on forests and their functioning through more frequent and intense periods of drought. Repeated conditions of water limitation not only affect the growth and vitality of trees but also feed back on the cycling of carbon (C) at the plant-soil interface. However, the impact of the intensity of drought on the transfer of assimilated C belowground remains quantitatively unresolved. We assessed how increasing levels of soil water limitation affect the growth of Scots pine (Pinus sylvestris L.) saplings and performed a 13C-CO2 pulse labelling experiment to trace the pathway of newly-assimilated C into needles, fine roots, soil pore CO2, and phospholipid fatty acids of soil microbial groups. We hypothesized that increased water stress would reduce tree C uptake, and the magnitude and velocity at which newly-assimilated C is allocated belowground and further metabolized. Moreover, we expected that severe levels of soil water deficit would lead to a build-up of newly-assimilated C in fine roots. Our data indicated that with more intense water limitation, trees reduced their growth despite initially partitioning more biomass to belowground tissues under severe water stress. Moderate levels of water limitation barely affected the uptake of 13C label and the magnitude and transit times of C being allocated from needles to the rhizosphere. In contrast, severe water limitation increased the fraction of 13C label allocated to roots and soil fungi while a lower fraction of 13CO2 was respired from the soil. We conclude that when soil water becomes largely unavailable, C cycling within trees becomes slower, and a major fraction of C allocated belowground is accumulated in roots or transferred to the soil and associated microorganisms without being metabolically used. Our experiment overall demonstrates the relevance of quantifying the level of water limitation at which C allocation dynamics within trees and soils are altered to inform about the trajectory of forests to the environmental pressures they face.

Nitrogen (N) plays a central role in marine biogeochemistry by regulating biological productivity, influencing the cycles of carbon, oxygen, and other nutrients, and controlling oceanic emissions of the potent greenhouse gas nitrous oxide (N2O). Although the marine N cycle consists of multiple chemical species, global biogeochemical models often employ simple parameterizations of N transformations and omit key tracers and processes. Here we present an extended numerical representation of the marine N cycle that includes explicit tracers for nitrate, dinitrogen, nitrous oxide, ammonium, and nitrite. The extended model simulates heterotrophic denitrification, DNRN, DNRA, anammox, and nitrification of ammonium and nitrite and thus allows for a detailed representation of a step-wise reduction of fixed nitrogen in hypoxic zones and oxidation of reduced N-species in oxic waters. The updated biogeochemical model is included in the new global ICOsahedral Non-hydrostatic Ocean model ICON-O developed at the Max Planck Institute for Meteorology. ICON-O features a flexible, triangular grid created by recursively subdividing the original 20 triangles of the icosahedron resulting, in our configuration, in an average resolution of 40 km and 235,403 triangles. We describe the tuning and spin-up of a pre-industrial control simulation and compare model results with global and local estimates to quantify the N cycle (e.g., rates of primary production, N2 fixation, nitrification, DNRN, DNRA, anammox). We further report results from a historical transient simulation focusing on N dynamics within the oxygen minimum zone of the Eastern Tropical South Pacific.

Communities in the highlands of Guatemala are currently struggling with insufficient access to effective sanitation. Water born solutions, often referred to as the “flush and forget model” of human excreta management, cannot be adequately delivered to the rapidly growing peri-urban regions growing across the area. The consequences of the insufficient collection and treatment of this waste are worsening human and environmental health outcomes. Concurrently, smallholder farmers in the region struggle to supply their soil with sufficient quantities of plant nutrients to avoid growing yield gaps. Even when capable of utilizing required amounts of chemical fertilizers, there is no clear option available to maintain soil organic carbon; typically relied upon organic inputs such as animal manure are available in only insufficient quantities.To deal with the sanitation challenge facing communities in the region, Mosan, an NGO based in the Lake Atitlan region of Guatemala has, for the last several years, piloted a novel approach to sanitation provision. Utilizing an on-site urine diversion system that focuses on the capture, processing, and valorization of excreta, this resource-oriented approach, in addition to providing households with the means to safely manage generated excreta, yields a novel organic fertilizer. Using two treatment processes, alkaline dehydration for urine and pyrolysis for the feces, Mosan can produce an enhanced biochar product that could have the potential to sustainably improve soil health and fertility for small holder highland farmers in the region. Working in partnership with Mosan and Vivamos Mejor, and agricultural development organization based in Guatemala, the Sustainable Agroecosystems group at ETH Zurich has been testing the potential of this novel source of organic fertilizer.Over the last eighteen months, this interdisciplinary team of researchers, community activists, and farmers has managed two experimental sites in the region. The first focused on the incorporation of enhanced biochar into a potting mix used to grow tree seedlings used for reforestation efforts in the region. The second, a participatory farmer field trial, was designed to compare the yield increases of maize fertilized with enhanced biochar to that grown with chemical fertilizer (urea). In addition to observing no significant differences in the growth performance of the seedlings, or the yield increases of the maize grown with the excreta-based biochar compared to the standard alternatives, our team also observed positive changes to several soil physical and chemical properties in the field trial. Given these results, we argue that a socio-technical transition towards a circular rural-urban system, one predicated on nutrient capture and reuse of currently underutilized organic waste sources such as human excreta, would simultaneously improve human and environmental health outcomes in urban areas, while also increasing long term soil health and fertility in outlying rural ones.

While the importance of soil organic carbon (SOC) in the global carbon cycle is well-established, many knowledge gaps remain related to how land management affects changes in SOC stocks, and protection mechanism of SOC. This is particularly the case for the tropics, as is clear from multiple recent meta-analyses on data related to soil biogeochemistry. In addition, while current knowledge on SOC dynamics is derived from the topsoil, studies on how land management affects subsoil OC properties are scarce.Therefore, we studied how two types of land management affect SOC characteristics down to 1 m depth in two locations in southern Kenya. At one location (Embu), the effect of nutrient management on protection mechanisms of SOC is assessed, while at the second location (Mau Forest region) we study the effect of land use changes on soil biogeochemistry. The focus of this study is on assessing how land management affects SOC protection mechanisms (using carbon fractionation) and the contribution of microbial necromass to total SOC (using amino sugar analysis). In addition, multiple other soil properties, including microbial ecology, have been quantified to improve our understanding of the effect of land management on subsoil OC dynamics.Using our results, we aim (i) to improve understanding of these processes and (ii) to use this knowledge to improve a mechanistic model simulating soil C and N dynamics along the soil profile.

Soil organic carbon (SOC) dynamics depend on soil properties derived from the geoclimatic conditions under which soils develop and are in many cases modified by land conversion. However, SOC stabilization and the responses of SOC to land use change are not well constrained in deeply weathered tropical soils, which are dominated by less reactive minerals than those in temperate regions. Along a gradient of geochemically distinct soil parent materials, we investigated differences in SOC stocks and SOC ( delta C-14) turnover time across soil profile depth between montane tropical forest and cropland situated on flat, non-erosive plateau landforms. We show that SOC stocks and soil delta C-14 patterns do not differ significantly with land use, but that differences in SOC can be explained by the physicochemical properties of soils. More specifically, labile organo-mineral associations in combination with exchangeable base cations were identified as the dominating controls over soil C stocks and turnover. We argue that due to their long weathering history, the investigated tropical soils do not provide enough reactive minerals for the stabilization of C input in either high input (tropical forest) or low-input (cropland) systems. Since these soils exceeded their maximum potential for the mineral related stabilization of SOC, potential positive effects of reforestation on tropical SOC storage are most likely limited to minor differences in topsoil without major impacts on subsoil C stocks. Hence, in deeply weathered soils, increasing C inputs may lead to the accumulation of a larger readily available SOC pool, but does not contribute to long-term SOC stabilization., Global Change Biology, 29 (9), ISSN:1354-1013, ISSN:1365-2486

By-product mutualisms are ubiquitous yet seldom considered in models of mutualism. Most models represent conditional mutualisms that shift between mutualism and antagonism in response to shifts in costs and benefits resulting from changes in environmental quality. However, in by-product mutualisms, benefits arise as a part of normal life processes and do not incur additional costs and do not have antagonistic alternate states. Recognizing that mutualisms are diverse, new, or more flexible, models are needed. We present a conceptual model that differs from traditional models in three ways: (1) partners exchange by-product benefits, (2) interactions do not have alternate antagonistic states, and (3) tradeoffs are allowed among factors that influence environmental quality. We applied this model to bark and ambrosia beetles that associate with fungi and that have developed two distinct pathways to by-product mutualism. We used independent axes for each major factor influencing environmental quality including those that exhibit tradeoffs. For these beetles, tradeoffs in nutrition and defense were key to which mutualism pathway was taken and the degree of fidelity that occurred among partners. This approach can be used to test hypotheses on the relative importance of the drivers underlying the development of by-product, or other, mutualisms.

IntroductionIn north-western France, Salmonella enterica susp. enterica serovar Mbandaka (S. Mbandaka) is most frequently isolated from bovine and dairy samples. While this serovar most often results in asymptomatic carriage, for a number of years it has caused episodes of abortions, which have serious economic consequences for the sector. Interestingly, this serovar is also isolated from Gallus gallus in the same geographic zone. Despite its prevalence in bovines in north-western France, S. Mbandaka has not been broadly studied at the genomic level, and its prevalence and host adaptation are still not fully understood.MethodsIn this study, we analyzed the genomic diversity of 304 strains of S. Mbandaka isolated from the bovine and poultry sectors in this area over a period of 5 years. A phylogenetic analysis was carried out and two approaches were followed to identify conserved genes and mutations related to host associations. The first approach targeted the genes compiled in the MEGARESv2, Resfinder, VFDB and SPI databases. Plasmid and phage contents were also investigated. The second approach refers to an in-house algorithm developed for this study that computes sensitivity, specificity, and accuracy of accessory genes and core variants according to predefined genomes groups.Results and discussionAll the analyzed strains belong to the multi-locus sequence type profile ST413, and the phylogenomic analysis revealed main clustering by host (bovine and poultry), emphasizing the circulation of 12 different major clones, of which seven circulate in poultry and five in the bovine sector in France and a likely food production chain adaptation of these clones. All strains present resistance determinants including heavy metals and biocides that could explain the ability of this serovar to survive and persist in the environment, within herds, and in food processing plants. To explore the wild animal contribution to the spread of this serovar in north-western France, we retrieved S. Mbandaka genomes isolated from wild birds from EnteroBase and included them in the phylogenomic analysis together with our collection. Lastly, screening of accessory genes and major variants allowed us to identify conserved specific mutations characteristic of each major cluster. These mutations could be used to design useful probes for food safety surveillance.

Supplemental Figure 3: Comparison between NHS/ HPFS CRC and COSMIC signatures Cosine similarity heat map between de novo signatures found in NHS/ HPFS (y axis) and COSMIC signatures (x axis). NHS/ HPFS signatures were named after their closest COSMIC mutational process match (in bold)

Supplemental Figure 1: Non-negative matrix factorization rank survey in NHS/HPFS Quality measures for Non-negative matrix factorization in NHS/ HPFS, as described in the R package "NMF". Arrows indicate the estimated rank of mutational signatures.

Supplemental Figure 5: Comparison between SigProfiler and standard NMF approach for TCGA Rank survey (left panels) and cosine similarity heatmaps between SigProfiler and standard NMF signatures (right panels) in TCGA.

Supplemental Figure 2: Comparison between SigProfiler and standard NMF approach for NHS/ HPFS Rank survey (left panels) and cosine similarity heatmaps between SigProfiler and standard NMF signatures (right panels) in NHS/ HPFS.

Supplemental Figure 12: MGMT promoter methylation status and red meat intake Red meat intake for NHS/ HPFS patients, stratified by MGMT promoter methylation status. The two-sided Mann Whitney U test p-value is indicated.

Immune cell deconvolution from molecular data. Relative proportions of immune cell subtypes are predicted from gene expression data using the Danaher method, and from methylation data using methylCIBERSORT. Differences between progressive and regressive groups are reported here.

Supplemental Figure 16: Impact of VAF on NMF rank selection The cophenetic score after NMF in NHS/HPFS was computed for different ranks (2 to 10, on the x axis) and different variant allele fraction thresholds (0 to 20).