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Background Breast magnetic resonance imaging (MRI) has high sensitivity in detecting invasive neoplasms. Controversy remains about its impact on the preoperative staging of breast cancer surgery. This study evaluated survival and surgical outcomes of preoperative MRI in conservative breast cancer surgery. Methods A phase III, randomized, open-label, single-center trial including female breast cancer participants, stage 0–III disease, and eligible for breast-conserving surgery. We compared the role of including MRI in preoperative evaluation versus radiologic exam routine with mammography and ultrasound in breast cancer conservative candidates. The primary outcome was local relapse-free survival (LRFS), and secondary outcomes were overall survival (OS), mastectomy rate, and reoperation rate. Results 524 were randomized to preoperative MRI group (n = 257) or control group (n = 267). The survival analysis showed a 5.9-years LRFS of 99.2% in MRI group versus 98.9% in control group (HR = 0.72; 95% CI 0.12—4.28; p = 0.7) and an OS of 95.3% in the MRI group versus 96.3% in the control group (HR = 1.37 95% CI 0.59–3.19; p = 0.8). Surgical management changed in 21 ipsilateral breasts in the MRI group; 21 (8.3%) had mastectomies versus one in the control group. No difference was found in reoperation rates, 22 (8.7%) in the MRI group versus 23 (8.7%) in the control group (RR = 1.002; 95% CI 0.57–1.75; p = 0.85). Conclusion Preoperative MRI increased the mastectomy rates by 8%. The use of preoperative MRI did not influence local relapse-free survival, overall survival, or reoperation rates.

No contexto do aumento significativo do número de casos de dengue, zika e chikungunya no Brasil e com a circulação simultânea dessas três doenças transmitidas pelo Aedes aegypti, torna-se necessária a implementação de estratégias educativas para prevenção e controle dessas doenças. Esse projeto de extensão teve como objetivo promover atividades de conscientização dessas arboviroses para estudantes do ensino médio em escolas públicas e particulares de Divinópolis-MG. Devido à situação de distanciamento social e cancelamento das atividades presenciais nas escolas em virtude da pandemia de COVID-19, foi criado um formulário no Google Forms contendo dois questionários e um vídeo elaborados pela equipe do projeto. Após o aceite de participação da escola por parte da direção, o formulário foi enviado via e-mail ou WhatsApp para o professor de biologia responsável que encaminhou o mesmo para os alunos. Um total de 1.486 alunos de 10 escolas de Divinópolis-MG participaram. Após a realização da atividade, foi elaborado um folder digital com informações sobre as arboviroses para o público-alvo e enviado para as escolas participantes. No questionário aplicado antes e após a apresentação do vídeo com perguntas sobre o tema, um aumento significativo no número de acertos foi observado em todas as perguntas. Em relação à avaliação pelo aluno sobre a atividade proposta, foi ressaltada a importância da apresentação do conteúdo e a qualidade metodológica. Nessa perspectiva, apesar dos desafios causados pela pandemia e pela situação de distanciamento social, a abordagem implementada foi considerada eficiente e apresentou importantes resultados no público-alvo, mostrando a importância da educação em saúde no ambiente escolar.

Arthropod-borne viruses (arboviruses), such as Zika virus (ZIKV), chikungunya virus (CHIKV), dengue virus (DENV), yellow fever virus (YFV), and West Nile virus (WNV), are pathogens of global importance. Therefore, there has been an increasing need for new drugs for the treatment of these viral infections. In this context, antimicrobial peptides (AMPs) obtained from animal venoms stand out as promising compounds because they exhibit strong antiviral activity against emerging arboviral pathogens. Thus, we systematically searched and critically analyzed in vitro and in vivo studies that evaluated the anti-arbovirus effect of peptide derivatives from toxins produced by vertebrates and invertebrates. Thirteen studies that evaluated the antiviral action of 10 peptides against arboviruses were included in this review. The peptides were derived from the venom of scorpions, spiders, wasps, snakes, sea snails, and frogs and were tested against DENV, ZIKV, YFV, WNV, and CHIKV. Despite the high structural variety of the peptides included in this study, their antiviral activity appears to be associated with the presence of positive charges, an excess of basic amino acids (mainly lysine), and a high isoelectric point (above 8). These peptides use different antiviral mechanisms, the most common of which is the inhibition of viral replication, release, entry, or fusion. Moreover, peptides with virucidal and cytoprotective (pre-treatment) effects were also identified. In conclusion, animal-venom-derived peptides stand out as a promising alternative in the search and development of prototype antivirals against arboviruses.

Introduction: Candida albicans is the most common causative agent of Intra-abdominal Candidiasis (IAC) and it is resistant to most antifungal drugs currently available. Here we investigated atorvastatin in vitro and in vivo antifungal activities against a fluconazole-resistant C. albicans strain as a potential repurposed drug. The following tests were carried out: antifungal susceptibility tests to determine minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), determination of time-kill curve, biofilm assays, Candida albicans yeast-hyphae transition inhibition assay, murine model of Intra-abdominal candidiasis, survival curve, fungal load quantification, histopathology analysis, quantification of TNF-α and IL-17 cytokines, quantification of N-acetyl-β-D-glucosaminidase. In vitro assays showed the synergetic action of atorvastatin and fluconazole against C. albicans growth and biofilm maturation while the time-kill curve assay revealed their fungicidal effect after 24 h of treatment. When yeast-to-hyphae transition was assessed, the synergetic effect of atorvastatin and fluconazole reduced C. albicans filamentation significantly. In vivo tests showed that one of the most noticeable signs of IAC is the intense systemic inflammation. However, our survival curve test showed that despite being ill, animals exhibited little to no clinical signs of systemic inflammation when treatment included a combination of atorvastatin and fluconazole. Altogether, these findings suggest that atorvastatin could be feasibly used in the treatment fluconazole-resistant C. albicans strains, showing that drug repurposing is an important strategy when considering the limited number of antifungal drugs available for treatment in addition to financial hardship experienced in research and development of new antifungal drugs.

IntroductionBees capable of performing floral sonication (or buzz-pollination) are among the most effective pollinators of blueberries. However, the quality of pollination provided varies greatly among species visiting the flowers. Consequently, the correct identification of flower visitors becomes indispensable to distinguishing the most efficient pollinators of blueberry. However, taxonomic identification normally depends on microscopic characteristics and the active participation of experts in the decision-making process. Moreover, the many species of bees (20,507 worldwide) and other insects are a challenge for a decreasing number of insect taxonomists. To overcome the limitations of traditional taxonomy, automatic classification systems of insects based on Machine-Learning (ML) have been raised for detecting and distinguishing a wide variety of bioacoustic signals, including bee buzzing sounds. Despite that, classical ML algorithms fed by spectrogram-type data only reached marginal performance for bee ID recognition. On the other hand, emerging systems from Deep Learning (DL), especially Convolutional Neural Networks (CNNs), have provided a substantial boost to classification performance in other audio domains, but have yet to be tested for acoustic bee species recognition tasks. Therefore, we aimed to automatically identify blueberry pollinating bee species based on characteristics of their buzzing sounds using DL algorithms.MethodsWe designed CNN models combined with Log Mel-Spectrogram representations and strong data augmentation and compared their performance at recognizing blueberry pollinating bee species with the current state-of-the-art models for automatic recognition of bee species.Results and DiscussionWe found that CNN models performed better at assigning bee buzzing sounds to their respective taxa than expected by chance. However, CNN models were highly dependent on acoustic data pre-training and data augmentation to outperform classical ML classifiers in recognizing bee buzzing sounds. Under these conditions, the CNN models could lead to automating the taxonomic recognition of flower-visiting bees of blueberry crops. However, there is still room to improve the performance of CNN models by focusing on recording samples for poorly represented bee species. Automatic acoustic recognition associated with the degree of efficiency of a bee species to pollinate a particular crop would result in a comprehensive and powerful tool for recognizing those that best pollinate and increase fruit yields.

A fascite necrotizante é uma infecção rara, de rápida progressão, causada por diversos microorganismos bacterianos, caracterizada por uma intensa destruição tissular. A atuação do enfermeiro nesses casos com uso do laser de baixa potência na cicatrização da lesão gerada é de suma importância, bem como a utilização da teoria de Dorothea Orem no processo de enfermagem, enaltecendo o autocuidado. Objetivo: Descrever a evolução da lesão por Gangrena de Fournier, através da utilização do laser de baixa potência. Método: Trata-se de um estudo com abordagem descritiva, do tipo relato de caso. A amostra foi composta por um cliente internado no setor de reabilitação de um hospital de retaguarda, com diagnóstico médico de Gangrena de Fournier, proveniente do hospital de agudos. A lesão foi mensurada semanalmente, com a utilização de régua graduada de folha sulfite descartável de 30 cm e registrada também por meio fotográfico. As sessões de aplicação do laser de baixa potência eram realizadas no próprio quarto do cliente. Resultados: Na admissão cliente apresentava lesão de Fournier em região inguinal direita se estendendo para bolsa escrotal e perianal, apresentando exposição de bolsa escrotal, com extensão de 37cm x 7,0cm x 4,0cm de profundidade, em que foram realizados aplicações de laser pontual e perpendicular, após 12 semanas e 2 dias de tratamento a lesão encontrava-se com extensão de 29x1,5cm com 0,5cm de profundidade, deste modo é perceptível a eficácia do laser no processo de cicatrização neste estudo. Em decorrência da lesão paciente foi submetido a colostomia temporária, no decorrer da internação foi constatado pela enfermagem um déficit em relação aos cuidados com a bolsa de colostomia pelo paciente, deste modo foi realizado uma busca ativa pelas dificuldades, medos e inseguranças do paciente, sendo realizado o planejamento e cuidados de enfermagem com base na Teoria de Orem. Conclusão: O uso do laser de baixa potência no tratamento de gangrena de Fournier apresentou-se eficaz neste estudo, ocasionando uma redução das áreas da lesão e no controle da dor, favorecendo uma melhora na qualidade de vida deste paciente, juntamente com o uso da teoria de Dorothea Orem é considerada uma ferramenta válida devido ao fato de proporcionar uma comunicação mais objetiva entre enfermeiro, cuidador e cliente, estabelecendo um planejamento da assistência de enfermagem que melhor se adequa, deste modo viabilizando uma assistência que venha empoderar o paciente quanto seu autocuidado.

Debates sobre Juventudes é uma obra escrita por muitas mãos que busca promover, avançar e tensionar o campo de pesquisa das juventudes contemporâneas. O livro contempla capítulos sobre políticas públicas para as juventudes, juventudes e trabalho, juventudes e religião, juventudes e política, juvenicídio, jovens agricultores, juventudes e educação, dentre outros temas. Entre conosco nesses debates e vamos construir, juntas/os/es, novos conhecimentos sobre as juventudes e suas múltiplas formas de ser e estar no mundo contemporâneo.

O presente trabalho se insere na temática acerca do profissional de apoio escolar na Educação Infantil, que é pessoa que exerce atividades de alimentação, higiene e locomoção da criança com deficiência e atua em todas as atividades escolares nas quais se fizer necessária. O objetivo da pesquisa é compreender como as publicações científicas abordam as ações educativas do profissional de apoio escolar, com o foco na Educação Infantil. Trata-se de uma pesquisa com abordagem qualitativa, do tipo bibliográfica. Para a busca dessas publicações foi utilizado o Portal Scientific Electronic Library Online (SciELO), o Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes), o Catálogo de Teses e Dissertações da Capes e a Biblioteca Digital Brasileira de Teses e Dissertações. Não foi utilizado nenhum parâmetro cronológico e como palavra-chave se utilizou “profissional de apoio escolar” ou “profissional de apoio”. Em um segundo momento da coleta de dados, foi utilizada as palavras “profissional de apoio escolar na Educação Infantil” para se fazer uma busca de trabalhos acadêmicos no mecanismo virtual de pesquisa do Google Acadêmico. Para a análise dos dados coletados foi utilizada a Análise de Conteúdo. Os resultados apontaram que há pesquisas que abordam a temática de uma maneira ampla, com foco nas legislações e na função do profissional de apoio escolar para apoiar a inclusão do aluno com deficiência na escola. Além disso, as pesquisas com foco na Educação Infantil evidenciaram que o profissional de apoio escolar representa um suporte para a inclusão da criança com deficiência, agindo como mediador entre a criança e as relações sociais, mas que se faz necessária a definição clara dos papéis dos profissionais da educação para o desenvolvimento efetivo da criança com deficiência.

Objectives This in vitro study was performed to evaluate fatigue survival by shear test in the union of leucite-reinforced feldspathic ceramic using different cement thicknesses. Materials and Methods Leucite-reinforced glass ceramics blocks were sectioned in 2-mm thick slices where resin cylinders were cemented. The samples were distributed in two experimental groups (n = 20) according to the cement thickness (60 and 300 μm). The specimens of each group were submitted to the stepwise fatigue test in the mechanical cycling machine under shear stress state, with a frequency of 2 Hz, a step-size of 0.16 bar, starting with a load of 31 N (1.0 bar) and a lifetime of 20,000 cycles at each load step. Results The samples were analyzed in a stereomicroscope and scanning electron microscopy to determine the failure type. There is no significant difference between the mean values of shear bond strength according to both groups. Log-rank (p = 0.925) and Wilcoxon (p = 0.520) tests revealed a similar survival probability in both cement layer thicknesses according to the confidence interval (95%). The fracture analysis showed that the mixed failure was the most common failure type in the 300-μm thickness group (80%), while adhesive failure was predominant in the 60-μm thickness group (67%). The different cement thicknesses did not influence the leucite ceramic bonding in fatigue shear testing; however, the thicker cement layer increased the predominance of the ceramic material failure. Conclusion The resin cement thicknesses bonded to leucite ceramic did not influence the long-term interfacial shear bond strength, although thicker cement layer increased the ceramic material cohesive failure. Regardless the cement layer thickness, the shear bond strength lifetime decreases under fatigue.

Zika virus (ZIKV) infections are associated with severe neurological complications and are a global public health concern. There are no approved vaccines or antiviral drugs to inhibit ZIKV replication. NS2B-NS3 protease (NS2B-NS3 pro), which is essential for viral replication, is a promising molecular target for anti-ZIKV drugs. We conducted a systematic review to identify compounds with promising effects against ZIKV; we discussed their pharmacodynamic and pharmacophoric characteristics. The online search, performed using the PubMed/MEDLINE and SCOPUS databases, yielded 56 articles; 7 relevant studies that reported nine promising compounds with inhibitory activity against ZIKV NS2B-NS3 pro were selected. Of these, five (niclosamide, nitazoxanide, bromocriptine, temoporfin, and novobiocin) are currently available on the market and have been tested for off-label use against ZIKV. The 50% inhibitory concentration values ​​of these compounds for the inhibition of NS2B-NS3 pro ranged at 0.38-21.6 µM; most compounds exhibited non-competitive inhibition (66%). All compounds that could inhibit the NS2B-NS3 pro complex showed potent in vitro anti-ZIKV activity with a 50% effective concentration ranging 0.024-50 µM. The 50% cytotoxic concentration of the compounds assayed using A549, Vero, and WRL-69 cell lines ranged at 0.6-1,388.02 µM and the selectivity index was 3.07-1,698. This review summarizes the most promising antiviral agents against ZIKV that have inhibitory activity against viral proteases. This article is protected by copyright. All rights reserved.

A disfagia e considerada um sintoma que promove interferencias na qualidade de vida de qualquer individuo, estando geralmente associada a complicacoes nutricionais e respiratorias que impactam o cliente em toda sua esfera biopsicossocial. Objetivo: Descrever a atuacao dos profissionais da area de enfermagem frente ao diagnostico de enfermagem de degluticao prejudicada. Percurso metodologico: Trata-se de um estudo descritivo do tipo relato de experiencia, realizado pelas enfermeiras de um hospital de reabilitacao de Campo Grande-MS, a partir das experiencias vivenciadas na gestao do cuidado de enfermagem com pacientes apresentando degluticao prejudicada, no periodo de maio a agosto de 2017. Resultados e discussao: A gestao dos cuidados de enfermagem para o diagnostico de degluticao prejudicada, deve ser realizada em conjunto com a equipe multidisciplinar, estando atento as mudancas ocorridas no mecanismo da degluticao dos clientes internados, seus sinais e sintomas, pois questoes pulmonares e nutricionais oriundas da disfagia comprometem ainda mais o quadro clinico desses individuos. O enfermeiro inserido na reabilitacao tem um papel de extrema importância nesta tematica, seja na sensibilizacao para o despiste da disfagia ou na aplicacao das varias tecnicas que possam melhorar o processo de degluticao, alem de atividades de supervisao e ensino de outros profissionais de saude e cuidadores informais sobre a tematica. Conclusao: Para a gestao do cuidado frente a degluticao prejudicada e necessario a intervencao de uma equipe multiprofissional, sendo o gerenciamento da disfagia e necessaria a intervencao de uma equipe que envolva diferentes formacoes profissionais, sendo imprescindivel a presenca do enfermeiro no processo, visto que o mesmo pode se destacar no processo de deteccao precoce da disfagia orofaringea, considerando que esse profissional passa a maior parte do tempo junto aos clientes.

Made available in DSpace on 2021-06-25T10:55:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01 Objective: Continuous efforts have been made to hasten the zirconia densification process without compromising properties. This study evaluated the long-term structural durability of microwave speed-sintered zirconia (MWZ) relative to a conventionally sintered zirconia (CZ). Methods: As-machined dental 3Y-TZP discs (Ø12 × 1.2 mm) were speed sintered at 1450 °C for 15 min using an industrial microwave oven, while conventional sintering was conducted in a standard dental furnace at 1530 °C for 2 h. Both were followed by natural cooling. The total sintering time was 105 min for MWZ and 600 min for CZ. Groups were compared regarding density, grain size, phase composition, and fracture resistance. Structural durability was investigated employing two fatigue protocols, step-stress and dynamic fatigue. Results: Compared to CZ, MWZ exhibited a slightly lower density (MWZ = 5.98 g/cm3, CZ = 6.03 g/cm3), but significantly smaller grain sizes (MWZ = 0.53 ± 0.09 μm, CZ = 0.89 ± 0.10 μm), lower cubic-zirconia contents (MWZ = 15.3%, CZ = 22.7%), and poorer translucency properties (TP) (MWZ = 13 ± 1, CZ = 29 ± 0.8). However, the two materials showed similar flexural strength (MWZ = 978 ± 112 MPa, CZ = 1044 ± 161 MPa). Additionally, step-stress testing failed to capture the fatigue effect in 3Y-TZP, whereas dynamic fatigue revealed structural degradation due to moisture-assisted slow-crack-growth (SCG). Finally, MWZ possessed a slightly higher Weibull modulus (MWZ = 7.9, CZ = 6.7) but similar resistance to SCG (MWZ = 27.5, CZ = 24.1) relative to CZ. Significance: Dental 3Y-TZP with similar structural durability can be fabricated six-times faster by microwave than conventional sintering. Department of Dental Materials and Prosthodontics Institute of Science and Technology of Sao Jose dos Campos Sao Paulo State University (UNESP), 777 Eng. Francisco Jose Longo Avenue Graduate Program in Dentistry Positivo University Department of Biomaterials and Biomimetics New York University College of Dentistry, 433 1st Ave. Department of Preventive and Restorative Sciences University of Pennsylvanian School of Dental Medicine, 240 S. 40th St. Department of Dental Materials and Prosthodontics Institute of Science and Technology of Sao Jose dos Campos Sao Paulo State University (UNESP), 777 Eng. Francisco Jose Longo Avenue

This study evaluated the effect of the extract and fractions of

Statement of problem: The trueness and precision of intraoral scanners (IOSs) and the effect of intracoronal restorations have been reported. However, studies addressing the accuracy of IOSs in reproducing different complete coverage onlay preparation designs are lacking. Purpose: The purpose of this in vitro study was to evaluate the influence of complete coverage onlay preparation design and intraoral scanning devices on the accuracy of digital scans in terms of trueness and precision. Material and methods: Three preparation designs on the mandibular first molar were considered: a traditional preparation design with isthmus reduction (IST), a traditional preparation design without isthmus reduction (wIST), and simplified nonretentive preparation (nRET). Digital scans of epoxy resin mandibular arch reference models of the preparations (containing second premolar, first molar, and second molar) were obtained by using 3 IOSs (iTero Element 2 [ELE], Trios 3 [TRI], and Primescan [PRI]) (n=10). Trueness (μm) and precision (μm) were analyzed by superimposing the digital scan on the digital reference models obtained with a high-accuracy industrial scanner (ATOS Core 80) in a tridimensional metrology software program. Accuracy was quantified by the absolute deviation (μm). Local and overall mean positive and negative deviations for trueness were also obtained. Data were analyzed by using the Kruskal-Wallis and Dunn tests with a statistical software program (α=.05). Results: The nonretentive preparation groups obtained higher trueness (3.8 μm) and precision (2.7 μm) than the IST and wIST groups (trueness=7.5 to 6.3 μm, precision=5.5 to 4.6 μm). Trueness values were lower with ELE×IST (16 μm), followed by ELE×wIST (13 μm), and PRI×IST (7.8 μm). In general, no difference was found between PRI and TRI scanners (6.3 to 5.9 μm), with lower performance for ELE (13 μm). Positive deviations were higher on the proximal box of the IST and wIST preparation and on the occlusal box of the IST group. Negative deviation was higher on the ELE×IST occlusal box. Conclusions: Different intraoral scanners and preparation designs influenced the accuracy of digital scans. A more complex preparation such as IST and wIST showed higher deviation. The iTero Element 2 scanner exhibited higher deviation for both trueness and precision.

Mayaro fever is an infection caused by Mayaro virus (MAYV) that stands out among the neglected diseases transmitted by arthropods. Brazil is the country with the highest number of confirmed cases of MAYV infection. However, epidemiological surveillance studies conducted in Brazil are decentralized and focus on small outbreaks and unconfirmed cases. Thus, the aim of this review was to determine the general epidemiological profile of MAYV infections in Brazil. Several medical databases (i.e., PUBMED/MEDLINE, Scopus, Cochrane Library, LILACS, SciELO, and Biblioteca Virtual em Saude) were searched for studies reporting cases of MAYV infections in Brazilian patients. Then, the rate of exposure to MAYV in Brazil was analyzed using RStudio® Software. We identified 37 studies published from 1957 to 2019, containing data of 12,374 patients from 1955 to 2018. The general rate of exposure to MAYV in Brazil was 10% (95% CI; 0.04-0.22), with 1,304 reported cases. The highest incidence of MAYV infection was found in the northern region (13%; 95% CI; 0.05-0.29), with 1,142 cases (88% of all cases). Furthermore, autochthonous MAYV cases have also been reported in the Central West (8%; 95% CI; 0.03-0.18) and Southeast (0.4%; 95% CI; 0.00-0.28). The states with the highest number of cases are Amazonas (490 cases), Para (276 cases), and Goias (87 cases). In conclusion, the general rate of exposure to MAYV in Brazil between 1955 and 2018 was considerable, especially in the Legal Amazon, in which 93% of cases were reported.

Made available in DSpace on 2022-04-28T19:30:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The production of specialty coffee has several factors and parameters that are added up in the course of production, so that the quality is expressed in the act of consumption. Based on this scenario, this study included the analysis of ten genotypes of arabica coffee, the materials being subjected to irrigated and rainfed water regimes, in a low altitude region, to identify responses for sensory and physical-chemical quality. The genotypes were evaluated in a split-plot scheme with a randomized block design, with three replications. Arabica coffee fruits were harvested with 80% cherry seeds and processed by the wet method. Subsequently, the characteristics related to physical-chemical and sensory analyses were evaluated. The genotypes of the Paraíso group showed great variability for the physical-chemical and sensory variables for rainfed and irrigated regimes. The genotypes of the Catuaí group, however, showed less variability for sensory characteristics in both cultivation environments and for physical-chemical characteristics in the irrigated regime. In the sensorial data set, the genotypes Catuaí 144 CCF and Catuaí 144 SFC (when irrigated) and Paraíso H 419-3-3-7-16-2, Paraíso H 419-3-3-7-16-11 and Catucaí 24-137 (rainfed cultivation), are more favourable to the production of specialty coffee at low altitude. Department of Agronomy Federal University of Viçosa Department of Agronomy and Food Sciences Federal University of Espirito Santo Federal Institute of Espírito Santo Instituto Capixaba de Pesquisa Assistência Técnica e Extensão Rural Department of Rural Engineering Faculty of Agricultural and Veterinary Sciences Paulista State University Júlio de Mesquita Filho Department of Rural Engineering Faculty of Agricultural and Veterinary Sciences Paulista State University Júlio de Mesquita Filho

The poetry of Pedro Kilkerry (1885-1917) is complex and unique, it is also complex to cir-cumscribe the Pedro Kilkerry’s archive in the Brazilian literary historiography. This article aims to appre-hend and reflect on the place occupied by the poet in the official archives of Brazilian literature. Based on Jacques Derrida’s (2001) notion of archiving, the archiving processes to which the poet’s work was submitted after his death are discussed. Seized by the desire, by the passion to recover the origin, one asks about the traits that distinguish the processes of unarchiving and archiving carried out in his work or even how to evaluate the role and impressions inscribed by the archons in the poet’s archive. When answering questions like these, the “archive fever” is questioned.

A hanseníase é uma doença de forte impacto na saúde pública brasileira, tanto pela dificuldade no diagnóstico, por parte dos profissionais de saúde, como pela possibilidade de recidiva anos após a apresentação inicial da doença. Este estudo teve como objetivo avaliar o perfil epidemiológico e clínico dos pacientes com casos de hanseníase no município de Sobral-CE, entre o período de 2012 a 2018, com análise especial para os casos de recidiva da doença. Os dados foram obtidos por meio do banco de dados do Sistema de Informação de Agravos de Notificação (SINAN) do município. Foram evidenciados 42 casos de hanseníase recidivante no período estudado, sendo mais da metade destes ocorridos em pessoas com faixa etária entre 41 e 70 anos de idade. Houve maior predominância de reincidência em pessoas do sexo feminino, da raça parda e com menor nível de escolaridade. A grande parte das recidivas foram classificadas como multibacilar e as formas clínicas predominantes foram dimorfa e virchowiana. Conclui-se que o perfil epidemiológico foi composto, em sua maioria, por pacientes de faixa etária mais avançada, raça parda e com baixa escolaridade. O perfil clínico predominante de doentes na recidiva foi com a forma multibacilar. Tais dados foram congruentes com os obtidos na literatura. Além disso, existe uma importante parcela de dados que não foi notificada corretamente no SINAN, dificultando a confiabilidade das informações.

Nowadays, the emergence of superbugs is one of the most serious public health problems. Strains of Staphylococcus aureus and Klebsiella pneumoniae, resistant to many antimicrobials available in clinical practice, have become increasingly common. The development of new drugs for the treatment of infections caused by these pathogens is necessary and urgent. A series of 11 analogs of marine 3-alkylpyridine alkaloids was evaluated in vitro for its antibacterial potential. Compound 6, a synthetic analog of viscosaline, exhibited significant activity against S. aureus, including an MRSA strain (MIC = 2 µg/mL). Additionally, it exhibited a 25% reduction in mature biofilm and a good selectivity index (SI > 16). None of the compounds exhibited activity against K. pneumoniae, but analog 6 was able to act in planktonic cells in the presence of polymyxin B at sub-inhibitory concentration. Compound 6 has promising anti-staphylococcal activity and future assays are encouraged to investigate their therapeutic potential.

Sugarcane bagasse has received attention as a raw material for the production of second-generation ethanol (E2G). However, its use is limited because of the cell wall recalcitrance, mostly conferred by lignin. Recently our knowledge of the genes coding for the enzymes of the lignin biosynthesis pathway has increased; however, still little is known about the transcription factors controlling the expression of these genes in sugarcane. Here we describe protocols to optimize the isolation of the promoters of the lignin biosynthetic genes ShCAD8, ShCOMT and ShF5H and the transcription factors (TFs) ShMYB85 and ShMYB58/63 in Saccharum species. To confirm whether these TFs are able to activate the target promoters, a transactivation assay in BY2 protoplasts of Nicotiana tabacum is also detailed.

A literatura relata que a infecção fúngica que mais acomete a cavidade oral dos seres humanos é a candidíase. Essa prevalência em larga escala tem relação com muitos casos de resistência aos antifúngicos, e falta de aderência ao tratamento devido os seus efeitos colaterais. O que sugere a necessidade de novas práticas para o tratamento desta patologia. Desta forma, a terapia fotodinâmica (PDT) tem inferido bons resultados no manejo e terapêutica da candidíase oral. O objetivo deste estudo foi verificar a eficácia da terapia fotodinâmica de forma isolada, e associada a terapia medicamentosa convencional. Método: revisão narrativa de literatura, à partir das bases de dados, MedLine, PubMed, Scielo, e Google acadêmico. Foram realizadas buscas em livros, artigos científicos, monografias, dissertações e teses, publicados entre 2011, e 2023. Os resultados inferem que o uso da PDT, como monoterapia possui competência para o controle da candidíase oral de maneira satisfatória. Contudo, verificou-se melhores resultados quando houve a associação da PDT, com a terapêutica convencional.

27 páginas.- 7 figuras.- 2 tablas.- 157 referencias.- Supplementary data The following supplementary data are available at JXB online., Phenolic metabolism is rewired during internode development and in response to cinnamyl alcohol dehydrogenase perturbation in sorghum, with different sets of genes and accumulating compounds. The molecular mechanisms associated with secondary cell wall (SCW) deposition in sorghum remain largely uncharacterized. Here, we employed untargeted metabolomics and large-scale transcriptomics to correlate changes in SCW deposition with variation in global gene expression profiles and metabolite abundance along an elongating internode of sorghum, with a major focus on lignin and phenolic metabolism. To gain deeper insight into the metabolic and transcriptional changes associated with pathway perturbations, a bmr6 mutant [with reduced cinnamyl alcohol dehydrogenase (CAD) activity] was analyzed. In the wild type, internode development was accompanied by an increase in the content of oligolignols, p-hydroxybenzaldehyde, hydroxycinnamate esters, and flavonoid glucosides, including tricin derivatives. We further identified modules of genes whose expression pattern correlated with SCW deposition and the accumulation of these target metabolites. Reduced CAD activity resulted in the accumulation of hexosylated forms of hydroxycinnamates (and their derivatives), hydroxycinnamaldehydes, and benzenoids. The expression of genes belonging to one specific module in our co-expression analysis correlated with the differential accumulation of these compounds and contributed to explaining this metabolic phenotype. Metabolomics and transcriptomics data further suggested that CAD perturbation activates distinct detoxification routes in sorghum internodes. Our systems biology approach provides a landscape of the metabolic and transcriptional changes associated with internode development and with reduced CAD activity in sorghum., This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) via the BIOEN Young Investigators Awards research grant (FAPESP no. 2015/02527-1). This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) -Finance Code 001, by FEDER/Agencia Estatal de Investigacion with the Spanish Projects AGL2017-83036-R and PID2020-118968RB-I00, and by Junta de Andalucia (Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, project P20_00017). IC is indebted to Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for the research fellowship 302927/2018-2. SSF was funded for a post-doctoral fellowship (FAPESP n0. 2016/06917-1). MSS was funded for a master's fellowship (FAPESP no. 2015/18361-5). AVAP was funded for a scientific initiation fellowship (FAPESP no. 2016/06714-3). LGAL was funded for a scientific initiation fellowship (FAPESP no. 2019/14604-1).

Although several aspects of lignin metabolism have been extensively characterized, the mechanism(s) by which lignin monomers are transported across the plasma membrane remains largely unknown. Biochemical, proteomic, expression and co-expression analyses from several plant species support the involvement of active transporters, mainly those belonging to the ABC superfamily. Here, we report on the genome-wide characterization of the ABCG gene subfamily in the model C4 grass Setaria viridis and further identification of the members potentially involved in monolignol transport. A total of 48 genes encoding SvABCGs were found in the S. viridis genome, from which 21 SvABCGs were classified as full-size transporters and 27 as half-size transporters. Comprehensive analysis of the ABCG subfamily in S. viridis based on expression and co-expression analyses support a role for SvABCG17 in monolignol transport: (i) SvABCG17 is orthologous to AtABCG29, a monolignol transporter in Arabidopsis thaliana; (ii) SvABCG17 displays a similar expression profile to that of lignin biosynthetic genes in a set of different S. viridis tissues and along the elongating internode; (iii) SvABCG17 is highly co-expressed with lignin-related genes in a public transcriptomic database; (iv) SvABCG17displays particularly high expression in the top of the S. viridis elongating internode, a tissue undergoing active lignification; (v) SvABCG17 mRNA localization coincides with the histochemical pattern of lignin deposition; and (vi) the promoter of SvABCG17 is activated by secondary cell wall-associated transcription factors, especially by lignin-specific activators of the MYB family. Further studies might reveal further aspects of this potential monolignol transporter, including its real substrate specificity and whether it works redundantly with other ABC members during S. viridis lignification.

Portulaca species can switch between C4 and crassulacean acid metabolism (CAM) depending on environmental conditions. However, the regulatory mechanisms behind this rare photosynthetic adaptation remain elusive. Using Portulaca oleracea as a model system, here we investigated the involvement of the circadian clock, plant hormones, and transcription factors in coordinating C4 and CAM gene expression. Free-running experiments in constant conditions suggested that C4 and CAM gene expression are intrinsically connected to the circadian clock. Detailed time-course, drought, and rewatering experiments revealed distinct time frames for CAM induction and reversion (days versus hours, respectively), which were accompanied by changes in abscisic acid (ABA) and cytokinin metabolism and signaling. Exogenous ABA and cytokinins were shown to promote and repress CAM expression in P. oleracea, respectively. Moreover, the drought-induced decline in C4 transcript levels was completely recovered upon cytokinin treatment. The ABA-regulated transcription factor genes HB7, NFYA7, NFYC9, TT8, and ARR12 were identified as likely candidate regulators of CAM induction following this approach, whereas NFYC4 and ARR9 were connected to C4 expression patterns. Therefore, we provide insights into the signaling events controlling C4–CAM transitions in response to water availability and over the day/night cycle, highlighting candidate genes for future functional studies in the context of facultative C4–CAM photosynthesis.

Prefabricated glass fiber-reinforced composite posts have been widely used because of their esthetic properties and favorable biomechanical behavior; however, because they are prefabricated, they may not adapt to the anatomy of all root canals. This case report describes a procedure for restoring teeth with a custom, 1-piece, fiber-reinforced composite post and core fabricated using computer-aided design/computer-aided manufacturing (CAD/CAM). The excellent adaptation of this custom endodontic post enables the use of a thinner, homogenous cement layer; in addition, it eliminates the need to construct a composite resin core build-up. Although more clinical and in vitro studies are needed to better define the appropriate clinical protocols and assess various materials, CAD/CAM technology allows post and core restorations to be manufactured in a predictable and simplified way.

The aim of this study was to evaluate the effect of four different finishing procedures on the fatigue strength of a fully stabilized zirconia (5Y-FSZ) material.Disc-shaped specimens of a 5Y-FSZ (Katana UTML, Kuraray Noritake) were made (ISO 6872-2015), grinded with 600- and 1200-grit silicon carbide paper, sintered as recommended, and randomly assigned into four groups according to the finishing technique: C (control, as-sintered), P (polished with polishing rubbers), G (glaze application - powder/liquid technique), and PG (polished with polishing rubbers + glaze application - powder/liquid). Then fatigue strength (staircase method), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses were performed.The C group presented the lowest fatigue strength, while the PG group presented the highest. The P and G groups presented intermediate behavior, presenting similar statistical results. XRD showed similar crystalline phase patterns for all groups. SEM images revealed some changes in the zirconia surface, with the P group presenting some scratches on the surface, while the scratches in the PG group were filled with the glaze material.None of the techniques analyzed in this study impaired the fatigue strength of fully stabilized zirconia. Importantly, the polishing rubbers combined with glaze application (PG group) improved its fatigue strength.The polishing rubbers followed by glaze application improve the fatigue strength in ultra-translucent zirconia.

As notícias falsas são uma arma de desinformação conhecida, capaz de ameaçar o estado democrático. Em um momento em que as mídias tradicionais são constantemente atacadas e acusadas de fazerem parte de uma grande conspiração para manter o poder das classes dominantes, as pessoas transformaram as redes sociais em sua fonte primária de informação, pois nelas existe menos controle sobre o que circula e, pretensamente, trazem menor risco de manipulação pelas mídias tradicionais. Na intersecção de fatores como a democratização na produção de conteúdo sem qualquer supervisão, a personalização das mensagens que não confrontam o usuário e o estímulo ao compartilhamento, as fake news encontram um campo para florescer e se propagar. Em 2017 elas evoluíram, com o nome de deepfake, deixaram de ser apenas mensagens no formato de texto, para contar com a manipulação de imagens, áudios e vídeos. Sua capacidade de forjar a realidade de maneira praticamente imperceptível, até mesmo para especialistas, chamou a atenção das mídias e da academia. Para discutir os efeitos e as ameaças dessa tecnologia, e como combatê-las, esse artigo, baseado na semiótica desenvolvida por Peirce, aponta para o esforço feito pelos produtores do vídeo In Event of Moon Disaster em criar um conteúdo educativo para alertar as pessoas sobre as consequências das deepfakes.

The hybrid abutment emerged as an alternative to maintain a stable and favorable volume of peri-implant tissue; it is composed of a mesostructure in ceramic and a metallic connection, which can be machined according to the peri-implant emergence profile. This case report presented the clinical steps for a dental implant with immediate provisional restoration, followed by the installation of implant-supported all-ceramic crowns with zirconia abutment and platform switching. A 40-year-old male patient was rehabilitated with an implant-supported ceramic crown in the region of element 11 and a ceramic veneer was cemented on the upper right central incisor. To do so, an atraumatic extraction of the upper left central incisor was performed followed by implant placement and immediate loading. In this case, a zirconia hybrid abutment was used with a metallic link in the cervical region with a concave platform switching base. This association of clinical steps enabled improving the aesthetics in the anterior dental region.

The NS5 methyltransferase (MTase) has been reported as an attractive molecular target for antivirals discovery against the Zika virus (ZIKV). Here, we report structure-based virtual screening of 42 390 structures from the Development Therapeutics Program (DTP) AIDS Antiviral Screen Database. Among the docked compounds, ZINC1652386 stood out due to its high affinity for MTase in comparison to the cocrystallized ligand MS2042, which interacts with the Asp146 residue in the MTase binding site by hydrogen bonding. Subsequent molecular dynamics simulations predicted that this compound forms a stable complex with MTase within 50 ns. Thus, ZINC1652386 may represent a promising ZIKV methyltransferase inhibitor.

The article proposes a reading of Pedro Kilkerry's "E o silencio...", with the aim of focusing on the issue of the poetic creation process present in the poem, not only as a theme, but above all as the dynamics itself embodied in writing. For this, we start from the concept of block of sensation (affects and percept), proposed by Gilles Deleuze and Felix Guattari, in relation to the concepts of territory and deterritorialization, of the same philosophers, in order to understand the movement of creation that is materialized in the poem. Based on a reinterpretation of Kilkerry poetry, we try to test the hypothesis that not only does the uniqueness of his poetry results from the original treatment he gives to language, but also from his ingenuity to aesthetically reconfigure, in the light of the phenomenon of alterity, the problem of subjectivity.

[Background] The increasing incidence of invasive forms of candidiasis and resistance to antifungal therapy leads us to seek new and more effective antifungal compounds. Objectives: To investigate the antifungal activity and toxicity as well as to evaluate the po-tential targets of 2-cyclohexylidenhydrazo-4-phenyl-thiazole (CPT) in Candida albicans., [Methods] The antifungal activity of CPT against the survival of C. albicans was inves-tigated in Caenorhabditis elegans. Additionally, we determined the effect of CPT on the inhibition of C. albicans adhesion capacity to buccal epithelial cells (BECs), the toxicity of CPT in mammalian cells, and the potential targets of CPT in C. albicans., [Results] CPT exhibited a minimum inhibitory concentration (MIC) value of 0.4-1.9 μg/mL. Furthermore, CPT at high concentrations (>60 x MIC) showed no or low toxici-ty in HepG2 cells and, [Conclusion] In conclusion, CPT appears to target the cell wall of C. albicans, exhibits low toxicity in mammalian cells, and prolongs the survival of C. elegans infected with C. albicans.

Summary Objective In this report, we investigated the effects of CitrusiM® (Citrus sinensis (L.) Osbeck dried extract) on body composition, defined in terms of two outcomes: percentage of lean mass and percentage of fat mass. Research methods & procedures A double-blind randomized experiment with overweight and obese volunteers was conducted in which the two variables were measured at baseline and three months afterwards. The treatment levels corresponded to three different daily doses of CitrusiM®: 0 mg (control), 0.5 g, and 1 g. All patients met with a nutritionist, and were given a standardized diet with their assigned treatments. At the end of three months, the intermediate outcomes of diet compliance (in percent) and amount of exercise (either none, low level, or high level) were recorded. The covariates that were collected for each subject were age, height, and sex. We analyzed the effects of the treatments on the two outcomes under a Bayesian framework of the Rubin Causal Model, and accounted for possible relationships between both diet compliance and exercise level and the three outcomes of interest in the analyses. Study registered under Research Ethics Committee of the Faculdade de Ciencias Medicas e da Saude de Juiz de For a (plataformabrasil.gov.br): Identifier no. 2.767.611. Results There was a statistically significant evidence that the treatment may increase lean mass and reduce fat mass. Conclusion A 3-month treatment with CitrusiM® (0.5 g or 1 g, daily) was able to increase lean mass, reduce fat mass, in the participants.

The DC-SIGN glycoprotein is responsible for the initial adhesion of dengue virus (DENV) to immune cells by the carbohydrate recognition domain (CRD). There are thirteen soluble and membrane-bound DC-SIGN isoforms, but the role of soluble isoforms in the DENV internalization process is not known. Five isoforms with an altered or absent CRD were identified, and three different soluble isoforms were used to confirm the interactions with mannose residues. The results show the loss of binding ability of one soluble isoform and binding ability of two of them. All of them will be used to verify their role in the DENV internalization process.

Nosocomial infections are an important cause of morbi-mortality worldwide. The increase in the rate of resistance to conventional drugs in these microorganisms has stimulated the search for new therapeutic options. The nitro moiety (NO2) is an important pharmacophore of molecules with high anti-infective activity. We aimed to synthesize new nitro-derivates and to evaluate their antibacterial and anti-Candida potential in vitro. Five compounds [3-nitro-2-phenylchroman-4-ol (3); 3-nitro-2-phenyl-2H-chromene (4a); 3-nitro-2-(4-chlorophenyl)-2H-chromene (4b); 3-nitro-2-(4-fluorophenyl)-2H-chromene (4c), and 3-Nitro-2-(2,3-dichlorophenyl)-2H-chromene (4d)] were efficiently synthesized by Michael-aldol reaction of 2-hydroxybenzaldehyde with nitrostyrene, resulting in one β-nitro-alcohol (3) and four nitro-olefins (4a–4d). The antibacterial and anti-Candida potentials were evaluated by assaying minimal inhibitory concentration (MIC), minimum fungicidal concentration (MFC), and minimum bactericidal concentration (MBC). Mono-halogenated nitro-compounds (4b and 4c) showed anti-staphylococcal activity with MIC values of 15.6–62.5 μg/mL and MBC of 62.5 μg/mL. However, the activity against Gram-negative strains was showed to be considerably lower and our data suggests that this effect was associated with the outer membrane. Furthermore, nitro-compounds 4c and 4d presented activity against Candida spp. with MIC values ranging from 7.8–31.25 μg/mL and MFC of 15.6–500 μg/mL. In addition, these compounds were able to induce damage in fungal cells increasing the release of intracellular material, which was associated with actions on the cell wall independent of quantitative changes in chitin and β-glucan. Together, these findings show that nitro-compounds can be exploited as anti-staphylococcal and anti-Candida prototypes.