1. A Rapid Quantitative lon-Exchange Extraction for 2,8-Dioxyadenine
- Author
-
Lken Mr, Wte Sh, and Shields Ce
- Subjects
Chromatography ,Ion exchange ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Extraction (chemistry) - Abstract
A rapid quantitative method for the determination of 2,8-dioxyadenine in biological fluids has been developed and evaluated in which a two-step HCI elution from Dowex-50W is used. The standard curve has a slope of 0.999 0.0647, and recovery is essentially 100%. In contrast to the two-day col-umn chromatographic technique previously avail-able, this procedure permits numerous determina-tions in less than an hour, which makes it appro-priate as a rapid screening assay for 2,8•dioxyade-nine whenever large doses of adenine are admin-istered. A variation of this procedure may be used as a semiquantitative test for adenine.
- Published
- 1970