1. FIRE mimics a 14-3-3-binding motif to promote Phytophthora palmivora infection
- Author
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Evangelisti, Edouard, Guyon, Alex, Shenhav, Liron, Schornack, Sebastian, Schornack, Sebastian [0000-0002-7836-5881], and Apollo - University of Cambridge Repository
- Subjects
Infectious Diseases ,Physiology ,General Medicine ,Infection ,Agronomy and Crop Science ,3108 Plant Biology ,31 Biological Sciences - Abstract
The oomycete Phytophthora palmivora infects a wide range of tropical crops worldwide. Like other filamentous plant pathogens, it secretes effectors to colonise plant tissues. Here we characterise FIRE, an RXLR effector that contains a canonical mode I 14-3-3 phospho-sensor binding motif that is conserved in effectors of several Phytophthora species. FIRE is phosphorylated in planta and interacts with multiple 14-3-3 proteins. Binding is sensitive to the R18 14-3-3 inhibitor. FIRE promotes plant susceptibility and co-localises with its target around haustoria. This work uncovers a new type of oomycete effector target mechanism. It demonstrates that substrate mimicry for 14-3-3 proteins is a cross-kingdom effector strategy used by both prokaryotic and eukaryotic plant pathogens to suppress host immunity.
- Published
- 2023
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