1. Cross-reactivity of Toxocariasis with Crude Antigen ofToxascaris leoninaLarvae by ELISA
- Author
-
Jin, Yan, Shen, Chenghua, Huh, Sun, Choi, Min-Ho, and Hong, Sung-Tae
- Subjects
Antibodies, Helminth ,Enzyme-Linked Immunosorbent Assay ,Cross Reactions ,medicine.disease_cause ,Cross-reactivity ,Serology ,Toxascaris ,Larvae ,medicine ,Animals ,Humans ,Helminths ,Toxascaris leonina ,Serodiagnosis ,Toxocariasis ,biology ,Toxocara canis ,General Medicine ,Infectious Diseases, Microbiology & Parasitology ,biology.organism_classification ,medicine.disease ,Serum IgG ,Crude Antigen ,Canis ,Antigens, Helminth ,Immunoglobulin G ,Larva ,Cross-reaction ,Immunology ,Original Article - Abstract
Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world. Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers. Recently, patients of clinically diagnosed toxocariasis are increasing and require correct diagnosis in Korea. The present study investigated serological cross-reactivity between crude antigens of T. canis (TCLA) and T. leonina (TLLA) larvae. We collected serum specimens from 177 toxocariasis patients who were clinically suspected in the Seoul National University Hospital and 115 healthy controls. An ELISA method for toxocariasis was used to evaluate diagnostic efficacy of TLLA for serodiagnosis of human toxocariasis. The IgG ELISA using TLLA gave 14 (14.3%) positives of 98 TCLA positive specimens among 177 suspected toxocariasis patients. Most of them showed high absorbances with TCLA. In conclusion, there is a partial cross reaction between serum specimens of toxocariasis and TLLA. Graphical Abstract
- Published
- 2015
- Full Text
- View/download PDF