237 results on '"Sei Kobayashi"'
Search Results
2. Efficacy of Early Tympanostomy Tube Insertion for Otitis Media with Effusion in Children with Down Syndrome
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Koichiro Oyake, Shuta Tomisato, Nozomi Takahashi, Yuzuru Okuba, Sei Kobayashi, and Noriko Morimoto
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- 2022
3. Inhibitory mechanism of tangeretin, a citrus flavone on the sphingosylphosphorylcholine (SPC)-induced vascular smooth muscle contraction
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Nan Li, Ying Zhang, Tomoka Morita, Hiroko Kishi, and Sei Kobayashi
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Pharmacology ,Citrus ,rho-Associated Kinases ,Sphingosine ,Swine ,Phosphorylcholine ,Animals ,Molecular Medicine ,Calcium ,Flavones ,Muscle, Smooth, Vascular ,Muscle Contraction - Abstract
We previously discovered that the SPC/Fyn/Rho-kinase (ROK) pathway mediates the Ca
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- 2022
4. Modulation of pancreatic tumor immunity by NKG2D T cells through MICB shedding
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Hitoshi Toyoda, Atsuo Kuramasu, Masahiro Hosonuma, Masakazu Murayama, Yoichiro Narikawa, Junya Isobe, Yuta Baba, Kohei Tajima, Eiji Funayama, Midori Shida, Yuya Hirasawa, Toshimitsu Tsurui, Hirotsugu Ariizumi, Tomoyuki Ishiguro, Risako Suzuki, Sei Kobayashi, Atsushi Horiike, Noriko Hida, Takehiko Sambe, Koji Nobe, Satoshi Wada, Hitome Kobayashi, Mayumi Tsuji, Shinichi Kobayashi, Takuya Tsunoda, Katsunori Inagaki, Yuji Kiuchi, and Kiyoshi Yoshimura
- Abstract
Natural killer group 2 member D ligands (NKG2DLs) are expressed as stress response proteins in cancer cells. NKG2DLs induce the activation of immune cells or tumor escape responses, depending on their expression. Human pancreatic cancer cells, Panc-1, express membrane MICA/B (mMICA/B), whereas soluble MICB (sMICB) is detected in the culture supernatant. We hypothesized that sMICB saturates NKG2D in NKG2DLow T cells and inhibits the activation signal from mMICB to NKG2D. The findings revealed that downregulation of MICB reduced sMICB, downregulated mMICB, maintained activation of NKG2DLow T cells, and inhibited activation of NKG2DHigh T cells. To maintain mMICB expression and reduce sMICB expression, we inhibited ADAM, a metalloproteinase that sheds MICB. Subsequently, the shedding of MICB was prevented by ADAM17 inhibitors, and the activation of NKG2DLow T cells was maintained. These results elucidate the mechanism of immune escape via sMICB and show promising potential for the activation of NKG2DLow T cells within the tumor microenvironment.
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- 2023
5. Hesperetin Inhibits Sphingosylphosphorylcholine-Induced Vascular Smooth Muscle Contraction by Regulating the Fyn/Rho-Kinase Pathway
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Qian Lu, Hiroko Kishi, Ying Zhang, Tomoka Morita, and Sei Kobayashi
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Pharmacology ,rho-Associated Kinases ,Sphingosine ,Swine ,Hesperidin ,Phosphorylcholine ,Animals ,Calcium ,Phosphorylation ,Cardiology and Cardiovascular Medicine ,Muscle, Smooth, Vascular ,Muscle Contraction - Abstract
Cardiovascular diseases are the leading cause of mortality and disability worldwide. We have previously found that sphingosylphosphorylcholine (SPC) is the key molecule leading to vasospasm. We have also identified the SPC/Src family protein tyrosine kinase Fyn/Rho-kinase (ROK) pathway as a novel signaling pathway for Ca2+ sensitization of vascular smooth muscle (VSM) contraction. This study aimed to investigate whether hesperetin can inhibit the SPC-induced contraction with little effect on 40 mM K+-induced Ca2+-dependent contraction and to elucidate the underlying mechanisms. Hesperetin significantly inhibited the SPC-induced contraction of porcine coronary artery smooth muscle strips with little effect on 40 mM K+-induced contraction. Hesperetin blocked the SPC-induced translocation of Fyn and ROK from the cytosol to the membrane in human coronary artery smooth muscle cells (HCASMCs). SPC decreased the phosphorylation level of Fyn at Y531 in both VSMs and HCASMCs and increased the phosphorylation levels of Fyn at Y420, myosin phosphatase target subunit 1 at T853, and myosin light chain (MLC) at S19 in both VSMs and HCASMCs, which were significantly suppressed by hesperetin. Our results indicate that hesperetin inhibits the SPC-induced contraction at least in part by suppressing the Fyn/ROK pathway, suggesting that hesperetin can be a novel drug to prevent and treat vasospasm.
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- 2022
6. A Case of Osler’s Disease with Arteriovenous Malformation in the Nasal Septal Submucosa
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Aya Izumoto, Yoichi Ikenoya, Kei Sakikawa, Naomi Imaizumi, Shohei Matsuura, Tomotaka Shimura, Shuhei Takiguchi, Taketoshi Nogaki, and Sei Kobayashi
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Otorhinolaryngology - Published
- 2022
7. A case of intracranial foreign body perforated through the nasal cavity
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Hiroyuki Gunji, Aya Izumoto, Kouichirou Oyake, Youichi Ikenoya, Taketoshi Nogaki, and Sei Kobayashi
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- 2022
8. A Study on Usefulness of Fine Needle Aspiration Cytology in Cases Undergoing Thyroid Surgery
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Kei Sakikawa, Shuhei Takiguchi, Aya Izumoto, Yukiko Inoue, Naomi Imaizumi, Koichiro Oyake, Tomotaka Shimura, Yoichi Ikenoya, Taketoshi Nogaki, Sei Kobayashi, and Hitome Kobayashi
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Otorhinolaryngology - Published
- 2022
9. Recovery from long-term severe visual impairment due to mucocele in the Onodi cell
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Taketoshi Nogaki, Sei Kobayashi, and Hitome Kobayashi
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- 2022
10. Differentiation between IgG4-related Mikulicz disease and Sjögren's syndrome: A review case report and literature review
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Yurie Otani, Tomotaka Shimura, Taketoshi Nogaki, Yoichi Ikenoya, Koichiro Oyake, Naomi Imaizumi, Yukiko Inoue, Shuhei Uruma, Sawa Kamimura, Yojiro Kawamura, and Sei Kobayashi
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Male ,Sjogren's Syndrome ,Otitis Media with Effusion ,Immunoglobulin G ,Humans ,General Medicine ,Immunoglobulin G4-Related Disease ,Mikulicz' Disease ,Aged ,Hearing Loss, Mixed Conductive-Sensorineural - Abstract
IgG4-related diseases cause lesions in various organs throughout the body. In otorhinolaryngology, IgG4-related Mikulicz's disease is suspected and diagnosed based on the presence of lesions of the head and neck, salivary and lacrimal gland enlargement, and bilateral sinus opacity concentrated on the maxillary sinuses. However, in some cases, it is necessary to consider about differentiation between IgG4-related Mikulicz's disease and Sjögren syndrome.A 75-years-old male patient visited our hospital with bilateral otitis media with effusion, which was resistant to conservative treatment. Other symptoms at presentation included enlarged bilateral submandibular and sublingual glands marked oral dryness, severe decrease in saliva secretion (1 mL/10 minutes), and dry eyes. We conducted a Schirmer's and fluorescent dye tests, both of which were positive. High serum IgG4 levels were observed, and although the Sjögren syndrome (SS)-A/SS-B antibodies were negative, marked hypolacrimation and tear secretion were observed. Therefore, a detailed examination considering both IgG4-related Mikulicz's disease and SS was conducted. Salivary gland scintigraphy performed prior to the salivary gland biopsy revealed a marked decrease in uptake, which satisfied the diagnostic criteria for SS; however, it was difficult to diagnose IgG4-related disease based on the diagnostic definition.Although a definitive diagnosis of SS was made, the persistent otitis media with effusion that was resistant to conservative treatment and bilateral mixed hearing loss were confirmed. As mixed hearing loss is considered an otological symptom of IgG4-related disease, oral steroid treatment was administered.Thereafter, marked recovery of hearing and reduced swelling and induration of the bilateral parotid and submandibular glands were observed. Clinically, IgG4-related Mikulicz's disease was strongly suspected, but a definite diagnosis of SS was made.In the absence of an IgG4-related Mikulicz's disease diagnosis, careful differentiation between IgG4-related Mikulicz's disease and 2 diseases and their diagnostic criteria was essential.
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- 2023
11. Impact of omalizumab on pollen‐induced seasonal allergic rhinitis: An observational study in clinical practice
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Shohei Matuura, Sei Kobayashi, Hitome Kobayashi, Akihiko Tanaka, Kojiro Hirano, Yuni Hirano, Shuhei Uruma, Isao Suzaki, Hideaki Watanabe, Mai Yano, and Takatoshi Tokudome
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medicine.medical_specialty ,business.industry ,Rhinitis, Allergic, Seasonal ,Omalizumab ,medicine.disease_cause ,Dermatology ,Clinical Practice ,Otorhinolaryngology ,Pollen ,Anti-Allergic Agents ,medicine ,Humans ,Immunology and Allergy ,Observational study ,business ,medicine.drug - Published
- 2021
12. Antimicrobial Stewardship Program for Patients in the Hematological Department Receiving Carbapenem Therapy: A Single-Center and Interrupted Time Series Analysis
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Ayako Suzuki, Fumihiro Yamaguchi, Masayuki Maeda, Miyuki Hashiguchi, Nobuyuki Kabasawa, Jun Sasaki, Tokutada Sato, Masaki Fuyama, Yohei Yamazaki, Kei Endo, Kae Iwata, Sei Kobayashi, and Hisato Fujihara
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Microbiology (medical) ,antimicrobial stewardship ,hematological disorders ,predictive factors ,Infectious Diseases ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,interrupted time series analysis ,Biochemistry ,Microbiology ,carbapenem - Abstract
As antibiotic resistance has become a global problem, the intervention of an antimicrobial stewardship team (AST) is warranted. In hematological disorders, infectious complications are crucial owing to abnormal neutrophil function and decreased cell-mediated immunity. Despite the widespread implementation of AST intervention, the effectiveness of stewardship practices for immunocompromised patients remains uncertain. We determined the effect of AST interventions on carbapenem therapy in the department of hematology. Patients admitted to the department and undergoing carbapenem therapy were enrolled. We compared carbapenem use between the pre-AST (April 2016–March 2018) and post-AST (April 2018–March 2021) periods. Factors associated with long-term carbapenem therapy were investigated. Overall, 515 episodes of carbapenem therapy in 264 patients in the department were evaluated. According to the interrupted time series analysis, the number of days of therapy decreased with AST intervention (β = −0.263, p = 0.011). In multivariate analysis, predictive factors associated with long-term carbapenem therapy (>8 days) were outpatient onset, chronic obstructive pulmonary disease, acute myeloid leukemia, multiple myeloma, and infection with resistant bacteria (such as extended spectrum β-lactamases and AmpC) (95% confidence interval, 1.030–2.818, 1.067–66.667, 1.057–2.782, 0.168–0.742, and 1.382–5.750, respectively). The AST intervention reduced carbapenem use in patients with hematological disorders.
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- 2023
13. Eicosapentaenoic acid ameliorates pulmonary hypertension via inhibition of tyrosine kinase Fyn
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Kaori Koga, Tetsuhiko Go, Mikiko Aoki, Ryuji Inoue, Hiroyasu Yokomise, Aya Yamamura, Kohtaro Abe, Katsuya Hirano, Lin Hai Kurahara, Narumi Aoki-Shoi, Sei Kobayashi, Eiji Yahiro, Satoh Toru, Hiroko Kishi, Keizo Hiraishi, Zhang Bo, Kaori Ishikawa, and Ying Zhang
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Male ,STAT3 Transcription Factor ,0301 basic medicine ,Hypertension, Pulmonary ,Myocytes, Smooth Muscle ,Pulmonary Artery ,030204 cardiovascular system & hematology ,Pharmacology ,SRC Family Tyrosine Kinase ,Proto-Oncogene Proteins c-fyn ,Mesoderm ,Rats, Sprague-Dawley ,Transforming Growth Factor beta2 ,03 medical and health sciences ,0302 clinical medicine ,FYN ,Animals ,Humans ,Medicine ,Phosphorylation ,STAT3 ,Molecular Biology ,Cell Proliferation ,Monocrotaline ,Hypertrophy, Right Ventricular ,Ventricular Remodeling ,biology ,Interleukin-6 ,business.industry ,Kinase ,Endothelial Cells ,medicine.disease ,Myocardial Contraction ,Survival Analysis ,Pulmonary hypertension ,Vasodilation ,src-Family Kinases ,030104 developmental biology ,Eicosapentaenoic Acid ,biology.protein ,Endothelium, Vascular ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Tyrosine kinase ,Vasoconstriction - Abstract
Pulmonary arterial hypertension (PAH) is a multifactorial disease characterized by pulmonary arterial vasoconstriction and remodeling. Src family tyrosine kinases, including Fyn, play critical roles in vascular remodeling via the inhibition of STAT3 signaling. EPA is known to inhibit Fyn kinase activity. This study investigated the therapeutic potential and underlying mechanisms of EPA and its metabolite, resolvin E1 (RvE1), to treat PAH using monocrotaline-induced PAH model rats (MCT-PAH), human pulmonary artery endothelial cells (HPAECs), and human pulmonary artery smooth muscle cells (HPASMCs). Administration of EPA 1 and 2 weeks after MCT injection both ameliorated right ventricular hypertrophy, remodeling and dysfunction, and medial wall thickening of the pulmonary arteries and prolonged survival in MCT-PAH rats. EPA attenuated the enhanced contractile response to 5-hydroxytryptamine in isolated pulmonary arteries of MCT-PAH rats. Mechanistically, the treatment with EPA and RvE1 or the introduction of dominant-negative Fyn prevented TGF-β2-induced endothelial-to-mesenchymal transition and IL-6-induced phosphorylation of STAT3 in cultured HPAECs. EPA and RvE1 suppressed Src family kinases' activity as evaluated by their phosphorylation status in cultured HPAECs and HPASMCs. EPA and RvE1 suppressed vasocontraction of rat and human PA. Furthermore, EPA and RvE1 inhibited the enhanced proliferation and activity of Src family kinases in HPASMCs derived from patients with idiopathic PAH. EPA ameliorated PAH's pathophysiology by mitigating vascular remodeling and vasoconstriction, probably inhibiting Src family kinases, especially Fyn. Thus, EPA is considered a potent therapeutic agent for the treatment of PAH.
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- 2020
14. Perivascular Adipose Tissue Is a Major Source of Nitric Oxide in Saphenous Vein Grafts Harvested via the No‐Touch Technique
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Toshiro Saito, Hiroshi Kurazumi, Ryo Suzuki, Kazumasa Matsunaga, Sarii Tsubone, Bochao Lv, Sei Kobayashi, Takashi Nagase, Takahiro Mizoguchi, Makoto Samura, Kotaro Suehiro, Takasuke Harada, Noriyasu Morikage, Akihito Mikamo, and Kimikazu Hamano
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coronary artery bypass grafting ,Atherosclerosis ,conventional preparation ,no‐touch technique ,Adipose Tissue ,nitric oxide ,saphenous vein graft ,RC666-701 ,Humans ,Diseases of the circulatory (Cardiovascular) system ,Saphenous Vein ,Cardiology and Cardiovascular Medicine ,Vascular Patency ,Dilatation, Pathologic - Abstract
Background Saphenous vein grafts (SVGs) are broadly used in coronary artery bypass grafting despite their inferior patency compared with arterial grafts. Recently, the no‐touch technique (NT), in which an SVG is harvested with a pedicle of perivascular adipose tissue (PVAT) without conduit distension, was shown to improve long‐term patency compared with conventional preparation (CV), wherein outer tissue is removed with distension. The NT was also reportedly associated with reduced atherosclerosis. Although endothelial damage provoked by conventional distension may underlie poor patency when CV is performed, the precise mechanisms underlying the salutary effects of the NT have been unclear. Methods and Results Residual SVGs prepared with CV (CV‐SVGs) or NT (NT‐SVGs) were obtained during coronary artery bypass grafting. Nitric oxide (NO 2 − /NO 3 − (NO x )) levels after 24 hours of tissue culture were quantified. The protein expression and localization were analyzed. The isometric force of SVG strips was measured. NT‐SVGs showed superior NO x production to CV‐SVGs. PVAT generated the majority of NO x in NT‐SVGs. PVAT highly expressed arginosuccinate synthase 1, a rate‐limiting enzyme in the molecular circuit for NO synthesis, thereby continuously providing the substrate for NO. A substantial level of endothelial NO synthase was also expressed in PVAT. Pharmacological inhibition of arginosuccinate synthase 1 or endothelial NO synthase significantly suppressed the NO x production in NT‐SVGs. PVAT induced vasorelaxation through NO production, even in the endothelium‐denuded SVG strips. Conclusions Preserving PVAT was predominantly involved in the superior NO x production in NT‐SVGs. Since NO plays crucial roles in suppressing atherosclerosis, this mechanism may greatly contribute to the excellent patency in NT‐SVGs.
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- 2022
15. Juvenile angiofibroma that could have been removed by endoscopic sinus surgery
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Taketoshi Nogaki, Shuhei Takiguchi, Koichiro Oyake, Sei Kobayashi, Yasuyuki Hinohira, Yuki Takeda, Hitome Kobayashi, and Naruo Shoji
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Endoscopic sinus surgery ,medicine.medical_specialty ,business.industry ,Medicine ,Juvenile angiofibroma ,business ,Surgery - Published
- 2020
16. A Case of Unicentric Castleman Disease Diagnosed by FDG-PET/CT
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Shuhei Takiguchi, Hitome Kobayashi, Naomi Imaizumi, Yukiko Inoue, Sei Kobayashi, and Koichiro Oyake
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medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,Castleman disease ,Medicine ,Fdg pet ct ,Radiology ,business ,medicine.disease - Published
- 2020
17. Paxillin controls actin stress fiber formation and migration of vascular smooth muscle cells by directly binding to the active Fyn
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Sei Kobayashi, Hiroko Kishi, Ying Zhang, and Tomoka Morita
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Stress fiber ,Vascular smooth muscle ,macromolecular substances ,Proto-Oncogene Proteins c-fyn ,environment and public health ,Biochemistry ,Muscle, Smooth, Vascular ,FYN ,Cell Movement ,Stress Fibers ,Genetics ,Humans ,Phosphorylation ,Molecular Biology ,Rho-associated protein kinase ,Actin ,Paxillin ,biology ,Chemistry ,Kinase ,Cell migration ,Coronary Vessels ,Actins ,Cell biology ,enzymes and coenzymes (carbohydrates) ,embryonic structures ,biology.protein ,biological phenomena, cell phenomena, and immunity ,Biotechnology - Abstract
Rho-kinase (ROK)-mediated migration of vascular smooth muscle cells plays a crucial role in cardiovascular diseases. Previously we demonstrated Fyn tyrosine kinase as an upstream molecule of ROK to mediate actin stress fiber formation that plays an important role in cell migration, but the molecular mechanism between the two kinases was unclear. To discover a novel signaling molecule that exists between Fyn and ROK, we identified paxillin acting downstream of the active Fyn by combined use of pulldown assay and mass spectrometry. Immunofluorescence staining confirmed co-localization of Fyn and paxillin at the ends of actin stress fibers in human coronary artery smooth muscle cells (CASMCs). Surface plasmon resonance assay demonstrated direct binding between constitutively active Fyn (CA-Fyn) and N-terminus of paxillin (N-pax). The sphingosylphosphorylcholine (SPC)-induced ROK activation, actin stress fiber formation and cell migration were inhibited by paxillin knockdown, which were rescued by full-length paxillin (FL-pax) but not N-pax. N-pax co-localized with CA-Fyn at the cytosol and overexpression of N-pax inhibited the SPC-induced actin stress fiber formation and cell migration, indicating that the direct binding of FL-pax and CA-Fyn at the ends of actin stress fibers is essential for the ROK-mediated actin stress fiber formation and cell migration. Paxillin, as a novel signalling molecule, mediates the SPC-induced actin stress fiber formation and migration in human CASMCs via the Fyn/paxillin/ROK signalling pathway by direct binding of active Fyn.
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- 2021
18. A case of a patient with IgG4+ plasma cell infiltration, an IgG4-related disease, localized to the auditory ossicles
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Tomotaka Shimura, Taketoshi Nogaki, Yoichi Ikenoya, Koichiro Oyake, Naomi Imaizumi, Yukiko Inoue, and Sei Kobayashi
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Otorhinolaryngology - Published
- 2022
19. Immunological effects of inosine, a metabolite from gut microbiota, in the tumor microenvironment
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Yoichiro Narikawa, Masahiro Hosonuma, Atsuo Kuramasu, Sei Kobayashi, Masakazu Murayama, Hitoshi Toyoda, Jyunya Isobe, Midori Shida, Takehiko Sambe, Mayumi Tsuji, Takuya Tsunoda, Shinichi Kobayashi, Toshikazu Shimane, Hitome Kobayashi, Yuji Kiuchi, and Kiyoshi Yoshimura
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Applied Mathematics ,General Mathematics - Published
- 2022
20. Add-on therapy with traditional Chinese medicine: An efficacious approach for lipid metabolism disorders
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Sei Kobayashi, Ying Zhang, and Hiroko Kishi
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0301 basic medicine ,Mediation (statistics) ,Lipid Metabolism Disorder ,Combination therapy ,Disease ,Traditional Chinese medicine ,030204 cardiovascular system & hematology ,Bioinformatics ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,Hyperlipidemia ,Humans ,Medicine ,Dyslipidemias ,Hypolipidemic Agents ,Pharmacology ,business.industry ,Cholesterol ,Cancer ,Lipid Metabolism ,medicine.disease ,Lipids ,Treatment Outcome ,030104 developmental biology ,chemistry ,Drug Therapy, Combination ,business ,Biomarkers ,Drugs, Chinese Herbal - Abstract
Add-on therapy with traditional Chinese medicine (TCM) has been extensively researched in the intractable diseases, such as asthma, cancer, and Alzheimer's disease. As an entirely new concept, add-on therapy of TCM has been also used to prevent and treat hyperlipidemia via lowering cholesterol level. However, the efficacy of add-on therapy with TCM for mediating lipid metabolism disorders remains controversial. In this review, we summarize and provide strong evidence that add-on therapy of TCM as a novel approach is efficacious and safe for hyperlipidemia associated diseases based on the mediation of lipid metabolism disorders.
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- 2018
21. The Nutritional Characteristics of the Hypotensive WASHOKU-modified DASH Diet: A Sub-analysis of the DASH-JUMP Study
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Junko Inagaki, Sei Kobayashi, Seiji Umemoto, Hiroko Kishi, Atsuko Kawamura, Makoto Mitarai, Katsuko Kajiya, and Hiroshi Oda
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,DASH diet ,Dietary Approaches To Stop Hypertension ,Blood Pressure ,030204 cardiovascular system & hematology ,DASH-JUMP ,Recommended Dietary Allowances ,Article ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Internal medicine ,Dash ,Internal Medicine ,Lifestyle medicine ,Humans ,Medicine ,Prospective Studies ,030212 general & internal medicine ,Eating habits ,Aged ,business.industry ,nutrient ,food ,Mean age ,Feeding Behavior ,Middle Aged ,Nutritional survey ,Treatment Outcome ,Blood pressure ,Hypertension ,Japanese ,Female ,Diet, Healthy ,business ,Nutritive Value ,human activities ,Niacin ,WASHOKU - Abstract
Background We developed a WASHOKU-modified DASH diet named DASH-JUMP. We previously reported the hypotensive effect of the DASH-JUMP diet in Japanese participants with untreated high-normal Blood Pressure (BP) or stage 1 hypertension. Objective We aim to introduce the DASH-JUMP diet worldwide as a new lifestyle medicine. Accordingly, we prospectively assessed the nutritional characteristics of the DASH-JUMP diet. Methods Participants were treated with the DASH-JUMP diet for 2 months. Then, for 4 months after the intervention, they consumed their usual diets. We conducted a nutritional survey using the FFQg nutrient questionnaire at baseline and after 1, 2, 3, and 6 months. We received completed questionnaires from 55 participants (28 men and 27 women; mean age 54.2 ± 8.0 years) and analyzed them. Results The DASH-JUMP diet is rich in green-yellow vegetables, seaweed, milk, and mushrooms, while it has low contents of meat, eggs, confectionery, oils and fats, pickles, shellfish boiled in sweetened soy sauce, and fruits. Nutrients significantly associated with the observed change in systolic BP were niacin (P = 0.005) and carbohydrate (P = 0.033). The results of the FFQg questionnaire revealed that participants who had an increased BP at 1 month after ceasing the intervention had eating habits that broadly imitated the DASH-JUMP diet at 4 months after ceasing the intervention. Therefore, the systolic and diastolic BP values at 4 months after ceasing the intervention decreased significantly compared to those at baseline. Conclusion The DASH-JUMP diet may represent a new lifestyle medicine for reducing hypertension.
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- 2018
22. A case of adenoid cystic carcinoma of tongue base
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Hiroshi Gomibuchi, Takehumi Yui, Shuhei Takiguchi, Toshikazu Shimane, and Sei Kobayashi
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Pathology ,medicine.medical_specialty ,Oncology ,Otorhinolaryngology ,business.industry ,Adenoid cystic carcinoma ,Medicine ,business ,medicine.disease ,Tongue Base - Published
- 2018
23. Addition of S-1 to radiotherapy for treatment of T2N0 glottic cancer: Results of the multiple-center retrospective cohort study in Japan with a propensity score analysis
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Yoshihiro Akazawa, Yoshihiro Yamada, Daisuke Sano, Teruhiko Tanabe, Yuji Tanigaki, Sei Kobayashi, Nobuhiko Oridate, Kenji Okami, Shunsuke Miyamoto, Akira Kubota, Masanori Komatsu, Ryo Ikoma, and Kazumasa Suzuki
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Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Glottis ,medicine.medical_treatment ,Population ,03 medical and health sciences ,0302 clinical medicine ,Laryngeal preservation ,Japan ,Internal medicine ,Medicine ,Humans ,030223 otorhinolaryngology ,education ,Propensity Score ,Laryngeal Neoplasms ,Aged ,Retrospective Studies ,Tegafur ,Aged, 80 and over ,education.field_of_study ,business.industry ,Incidence (epidemiology) ,Retrospective cohort study ,Middle Aged ,Progression-Free Survival ,Radiation therapy ,Drug Combinations ,Oxonic Acid ,Glottic cancer ,030220 oncology & carcinogenesis ,Propensity score matching ,Female ,Oral Surgery ,business ,Chemoradiotherapy - Abstract
Objectives This multicenter retrospective cohort study aimed to evaluate the significance of adding S-1 to radiotherapy (RT) for the treatment of T2N0 glottic cancer using a propensity score matched analysis in Japan. Materials and Methods This study was conducted on 287 patients with T2N0 glottic cancer who were treated with definitive RT or chemoradiotherapy with S-1 (S-1 RT) between April 2007 and March 2017. Propensity score matched analysis was performed to ensure the well-balanced characteristics of the groups of patients who received RT alone and S-1 RT. Overall, progression-free and laryngectomy-free survivals and local control and laryngeal preservation rates were compared. Results Fifty-four pairs of patients were selected after performing propensity score matched analysis. Clinical characteristics were well-balanced between the two groups. The overall survival of patients in the S-1 RT group was significantly better than those in the RT alone group (P = 0.008). The progression-free and laryngectomy-free survivals of patients in the S-1 RT group were also better than those in the RT alone group; however, the differences were not significant. In contrast, patients in the S-1 RT group had slightly lower local control and laryngeal preservation rates compared with those in the RT alone group. The incidence of dermatitis in the S-1 RT group was significantly higher than that in the RT alone group in the matched population (P = 0.013). Conclusions The addition of S-1 to RT for the treatment of T2N0 glottic cancer was not associated with better local control and laryngeal preservation rates in this study.
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- 2019
24. Effects of the DASH-JUMP dietary intervention in Japanese participants with high-normal blood pressure and stage 1 hypertension: an open-label single-arm trial
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Sei Kobayashi, Makoto Mitarai, Hiroshi Oda, Katsuko Kajiya, Seiji Umemoto, Junko Inagaki, Hiroko Kishi, and Atsuko Kawamura
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Blood Glucose ,Male ,medicine.medical_specialty ,DASH diet ,Physiology ,medicine.medical_treatment ,Health Behavior ,030204 cardiovascular system & hematology ,Body Mass Index ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Vegetables ,Dash ,Internal Medicine ,Insulin ,Humans ,Medicine ,030212 general & internal medicine ,Stage (cooking) ,Life Style ,business.industry ,blood pressure ,Feeding Behavior ,Middle Aged ,Dietary Approaches to Stop Hypertension diet ,Diet ,Clinical trial ,Treatment Outcome ,Blood pressure ,Hypertension ,Japanese ,Physical therapy ,Patient Compliance ,Female ,Original Article ,Open label ,Cardiology and Cardiovascular Medicine ,business ,lifestyle-related disease ,Body mass index - Abstract
The Dietary Approaches to Stop Hypertension (DASH) diet is recommended by the American Heart Association to lower blood pressure (BP); however, its effects in Japanese participants have not been rigorously studied. We assessed the effects of the DASH-Japan Ube Modified diet Program (DASH-JUMP), a modified DASH diet, on cardiometabolic and inflammatory biomarkers in Japanese participants with untreated high-normal BP or stage 1 hypertension. Fifty-eight participants (30 men and 28 women; mean age 54.1±8.1 years) with untreated high-normal BP or stage 1 hypertension followed the DASH-JUMP (salt 8.0 g per day) for 2 months. After the intervention period, they resumed their usual diets for 4 months. The DASH-JUMP significantly decreased the participants' body mass index values (24.6±3.5 kg m−2 at baseline→23.2±3.3 kg m−2 at 2 months, P=0.000), BP (153±14/91±11 mm Hg at baseline→130±16/80±9 mm Hg at 2 months, P=0.000 and 139±16/85±10 mm Hg at 6 months, P=0.000), fasting serum glucose level (100±26 mg dl−1→94±15 mg dl−1 at 2 months, P=0.003) and fasting insulin level (6.9±5.9 μIU ml−1→4.4±2.7 μIU ml−1 at 2 months, P=0.000). The mean compliance of the participants for the DASH-JUMP diet was 88.5%. The DASH-JUMP diet reduced cardiovascular risk factors and may be an effective nutritional strategy for preventing cardiovascular events.
- Published
- 2016
25. Quantification of Cell Migration and Invasion, and Their Association with Periostin in Anaplastic Thyroid Cancer, Using a Real-time Cell Analyzer
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Yoshiko Kudo, Takeshi Hayashi, Takehiko Sambe, Shunya Egawa, Takahiro Kobayashi, Kenichiro Kawaguchi, Masayuki Miyazawa, Shinichi Iwai, Sei Kobayashi, and Katsuji Oguchi
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,business.industry ,Cell migration ,Periostin ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Medicine ,Anaplastic thyroid cancer ,business ,Cell analyzer - Published
- 2016
26. Eight Cases of Surgical Glottic Closure for Severe Aspiration
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Shunya Egawa, Kenichiro Ikeda, Hitome Kobayashi, Yoichi Ikenoya, Yukiomi Kushihashi, Go Takahashi, Sei Kobayashi, Masako Terasaki, Toshikazu Shimane, and Yurika Kimura
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medicine.medical_specialty ,business.industry ,medicine ,Closure (topology) ,business ,Surgery - Published
- 2016
27. Role of Gremlins in the Aortic Arch of Spontaneously Hypertensive and Hyperlipidemic Rats
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Masayuki Oda, Katsuji Oguchi, Kenichiro Kawaguchi, Masayuki Miyazawa, Shinichi Iwai, Naoki Matsumoto, Toshio Kumai, and Sei Kobayashi
- Subjects
0301 basic medicine ,Aortic arch ,03 medical and health sciences ,medicine.medical_specialty ,030104 developmental biology ,business.industry ,Internal medicine ,medicine.artery ,Cardiology ,Medicine ,business - Published
- 2016
28. Fatty acid-binding protein 7 regulates function of caveolae in astrocytes through expression of caveolin-1
- Author
-
Yui Yamamoto, Yuki Yasumoto, Toyoshi Fujimoto, Takeo Yoshikawa, Yuji Owada, Yoshiteru Kagawa, Hiroko Kishi, Sei Kobayashi, Tomoo Sawada, Eiichi Takaki, Majid Ebrahimi, Hirofumi Miyazaki, Motoko Maekawa, Kazem Sharifi, Akira Nakai, Hiroshi Kogo, and Ariful Islam
- Subjects
Biology ,FABP7 ,Fatty acid-binding protein ,Cell biology ,Cellular and Molecular Neuroscience ,medicine.anatomical_structure ,Neurology ,Biochemistry ,Caveolae ,Caveolin 1 ,medicine ,TLR4 ,lipids (amino acids, peptides, and proteins) ,Signal transduction ,Lipid raft ,Astrocyte - Abstract
Fatty acid-binding proteins (FABPs) bind and solubilize long-chain fatty acids, controlling intracellular lipid dynamics. FABP7 is expressed by astrocytes in the developing brain, and suggested to be involved in the control of astrocyte lipid homeostasis. In this study, we sought to examine the role of FABP7 in astrocytes, focusing on plasma membrane lipid raft function, which is important for receptor-mediated signal transduction in response to extracellular stimuli. In FABP7-knockout (KO) astrocytes, the ligand-dependent accumulation of Toll-like receptor 4 (TLR4) and glial cell-line-derived neurotrophic factor receptor alpha 1 into lipid raft was decreased, and the activation of mitogen-activated protein kinases and nuclear factor-κB was impaired after lipopolysaccharide (LPS) stimulation when compared with wild-type astrocytes. In addition, the expression of caveolin-1, not cavin-1, 2, 3, caveolin-2, and flotillin-1, was found to be decreased at the protein and transcriptional levels. FABP7 re-expression in FABP7-KO astrocytes rescued the decreased level of caveolin-1. Furthermore, caveolin-1-transfection into FABP7-KO astrocytes significantly increased TLR4 recruitment into lipid raft and tumor necrosis factor-α production after LPS stimulation. Taken together, these data suggest that FABP7 controls lipid raft function through the regulation of caveolin-1 expression and is involved in the response of astrocytes to the external stimuli. GLIA 2015;63:780-794.
- Published
- 2015
29. A Study on Patients with Suspected Schwannoma of the Neck
- Author
-
Sei Kobayashi, Yukiomi Kushihashi, Yojiro Kawamura, Toshikazu Shimane, Yuko Shimotatara, Yoichi Ikenoya, Naokazu Fujii, and Taisuke Nakamura
- Subjects
Cervical tumor ,medicine.medical_specialty ,Otorhinolaryngology ,business.industry ,medicine ,Radiology ,Schwannoma ,medicine.disease ,business - Published
- 2015
30. Application of Mass Spectrometry for Signal Transduction Analysis
- Author
-
Sei Kobayashi and Hiroko Kishi
- Subjects
Chromatography ,Chemistry ,General Medicine ,Signal transduction ,Mass spectrometry - Published
- 2015
31. Cervical Sympathetic Chain Schwannomas
- Author
-
Yukiomi Kushihashi, Tatsuo Shirota, Hideyuki Katsuta, Kenichiro Ikeda, Sei Kobayashi, Yuya Kurasawa, Atsutoshi Yaso, Yoichi Ikenoya, Manabu Kitano, Shunya Egawa, and Toshikazu Shimane
- Subjects
Pathology ,medicine.medical_specialty ,Cervical sympathetic chain ,business.industry ,Medicine ,Schwannoma ,business ,medicine.disease - Published
- 2015
32. Phosphorylation of the Kinase Domain Regulates Autophosphorylation of Myosin IIIA and Its Translocation in Microvilli
- Author
-
Shigeru Komaba, Sei Kobayashi, Byung Chull An, Reiko Ikebe, Jin Young Kim, Mistuo Ikebe, Hiroko Kishi, and Tsuyoshi Sakai
- Subjects
Models, Molecular ,Threonine ,Myosin light-chain kinase ,macromolecular substances ,Biology ,Myosin IIIA ,Microfilament ,Biochemistry ,Article ,03 medical and health sciences ,0302 clinical medicine ,Catalytic Domain ,Okadaic Acid ,Myosin ,Animals ,Humans ,Myosin Type III ,Protein Interaction Domains and Motifs ,Enzyme Inhibitors ,Phosphorylation ,Kinase activity ,Furans ,030304 developmental biology ,0303 health sciences ,Microvilli ,Myosin Heavy Chains ,Protein Stability ,Autophosphorylation ,Actin remodeling ,Lipids ,Recombinant Proteins ,Cell biology ,Actin Cytoskeleton ,Protein Transport ,Enterocytes ,Amino Acid Substitution ,Protein kinase domain ,Mutant Proteins ,Rabbits ,Caco-2 Cells ,Protein Processing, Post-Translational ,030217 neurology & neurosurgery - Abstract
Motor activity of myosin III is regulated by autophosphorylation. To investigate the role of the kinase activity on the transporter function of myosin IIIA (Myo3A), we identified the phosphorylation sites of kinase domain (KD), which is responsible for the regulation of kinase activity and thus motor function. Using mass spectrometry, we identified six phosphorylation sites in the KD, which are highly conserved among class III myosins and Ste20-related misshapen (Msn) kinases. Two predominant sites, Thr¹⁸⁴ and Thr¹⁸⁸, in KD are important for phosphorylation of the KD as well as the motor domain, which regulates the affinity for actin. In the Caco2 cells, the full-length human Myo3A (hMyo3AFull) markedly enlarged the microvilli, although it did not show discrete localization within the microvilli. On the other hand, hMyo3AFull(T184A) and hMyo3AFull(T188A) both showed clear localization at the microvilli tips. Our results suggest that Myo3A induces large actin bundle formation to form microvilli, and phosphorylation of KD at Thr¹⁸⁴ and Thr¹⁸⁸ is critical for the kinase activity of Myo3A, and regulation of Myo3A translocation to the tip of microvilli. Retinal extracts potently dephosphorylate both KD and motor domain without IQ motifs (MDIQo), which was inhibited by okadaic acid (OA) with nanomolar range and by tautomycetin (TMC) with micromolar range. The results suggest that Myo3A phosphatase is protein phosphatase type 2A (PP2A). Supporting this result, recombinant PP2Ac potently dephosphorylates both KD and MDIQo. We propose that the phosphorylation-dephosphorylation mechanism plays an essential role in mediating the transport and actin bundle formation and stability functions of hMyo3A.
- Published
- 2014
33. Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca2+-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation
- Author
-
Min Zhang, Sei Kobayashi, Bochao Lyu, Ying Zhang, and Hiroko Kishi
- Subjects
Multidisciplinary ,Contraction (grammar) ,Myosin light-chain kinase ,Vascular smooth muscle ,fungi ,030204 cardiovascular system & hematology ,Biology ,Eicosapentaenoic acid ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Biochemistry ,chemistry ,medicine ,Docosapentaenoic acid ,medicine.symptom ,Vascular smooth muscle contraction ,Rho-associated protein kinase ,030217 neurology & neurosurgery ,Muscle contraction - Abstract
We previously reported that eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid (n-3 PUFA), effectively inhibits sphingosylphosphorylcholine (SPC)-induced Ca2+-sensitization of vascular smooth muscle (VSM) contraction which is a major cause of cardiovascular and cerebrovascular vasospasm, and EPA is utilized clinically to prevent cerebrovascular vasospasm. In this study, we clearly demonstrate that docosapentaenoic acid (DPA), which exists in two forms as omega-3 (n-3) and omega-6 (n-6) PUFA, strongly inhibits SPC-induced contraction in VSM tissue and human coronary artery smooth muscle cells (CASMCs), with little effect on Ca2+-dependent contraction. Furthermore, n-3 and n-6 DPA inhibited the activation and translocation of Rho-kinase from cytosol to cell membrane. Additionally, SPC-induced phosphorylation of myosin light chain (MLC) was inhibited in n-3 and n-6 DPA pretreated smooth muscleVSM cells and tissues. In summary, we provide direct evidence that n-3 and n-6 DPA effectively equally inhibits SPC-induced contraction by inhibiting Rho-kinase activation and translocation to the cell membrane.
- Published
- 2017
34. A stem cell medium containing neural stimulating factor induces a pancreatic cancer stem-like cell-enriched population
- Author
-
Shoichi Hazama, Nobuaki Suzuki, Noriko Maeda, Koichi Yoshikawa, Kouhei Sonoda, Sou Matsukuma, Yoshitaro Shindo, Ryoichi Tsunedomi, Yusaku Watanabe, Koji Tamada, Kiyoshi Yoshimura, Masaaki Oka, Sei Kobayashi, Hideyuki Saya, Atsuo Kuramasu, and Shinsuke Kanekiyo
- Subjects
cancer stem cells ,Cancer Research ,Epithelial-Mesenchymal Transition ,pancreatic cancer ,Population ,Biology ,Neural Stem Cells ,Cancer stem cell ,Cell Line, Tumor ,Pancreatic cancer ,medicine ,Humans ,education ,education.field_of_study ,Mesenchymal stem cell ,Articles ,Flow Cytometry ,medicine.disease ,Neural stem cell ,Culture Media ,Cell biology ,Pancreatic Neoplasms ,cell culture method ,Oncology ,Cell culture ,Neoplastic Stem Cells ,Intercellular Signaling Peptides and Proteins ,Stem cell ,Leukemia inhibitory factor - Abstract
Cancer stem cells (CSCs) have been studied for their self-renewal capacity and pluripotency, as well as their resistance to anticancer therapy and their ability to metastasize to distant organs. CSCs are difficult to study because their population is quite low in tumor specimens. To overcome this problem, we established a culture method to induce a pancreatic cancer stem-like cell (P-CSLC)-enriched population from human pancreatic cancer cell lines. Human pancreatic cancer cell lines established at our department were cultured in CSC-inducing media containing epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), neural cell survivor factor-1 (NSF-1), and N-acetylcysteine. Sphere cells were obtained and then transferred to a laminin-coated dish and cultured for approximately two months. The surface markers, gene expression, aldehyde dehydrogenase (ALDH) activity, cell cycle, and tumorigenicity of these induced cells were examined for their stem cell-like characteristics. The population of these induced cells expanded within a few months. The ratio of CD24high, CD44high, epithelial specific antigen (ESA) high, and CD44variant (CD44v) high cells in the induced cells was greatly enriched. The induced cells stayed in the G0/G1 phase and demonstrated mesenchymal and stemness properties. The induced cells had high tumorigenic potential. Thus, we established a culture method to induce a P-CSLC-enriched population from human pancreatic cancer cell lines. The CSLC population was enriched approximately 100-fold with this method. Our culture method may contribute to the precise analysis of CSCs and thus support the establishment of CSC-targeting therapy.
- Published
- 2014
35. Interaction of peptide-bound beads with lipopolysaccharide and lipoproteins
- Author
-
Hiroko Kishi, Sei Kobayashi, Tomomi Kobayashi, Megumi Matsumoto, Takashi Takagi, Akio Nakamura, Hiroyuki Omi, and Masatsugu M. Suzuki
- Subjects
Lipopolysaccharides ,Microbiology (medical) ,Phage display ,Lipopolysaccharide ,Apolipoprotein B ,Lipoproteins ,Sodium ,chemistry.chemical_element ,Peptide ,Sodium Chloride ,Sensitivity and Specificity ,Microbiology ,Plasma ,chemistry.chemical_compound ,Humans ,Sodium dodecyl sulfate ,Molecular Biology ,Polyacrylamide gel electrophoresis ,Limulus Test ,chemistry.chemical_classification ,Chromatography ,biology ,Temperature ,Microspheres ,chemistry ,Limulus amebocyte lysate ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Peptides ,Blood Chemical Analysis ,Protein Binding - Abstract
We previously reported the generation of lipopolysaccharide (LPS)-binding peptides by phage display and chemical modification. Among them, a dodecapeptide designated Li5-025 (K'YSSSISSIRAC'; K' and C' denote d-lysine and d-cysteine, respectively) showed a high binding affinity for LPS and was resistant to protease digestion (Suzuki et al., 2010). In the current study, Li5-025-bound silica beads, hereafter referred to as P-beads, were generated and found to be devoid of LPS-neutralizing activity. Thus, LPS bound to the P-beads could be directly used in the Limulus amebocyte lysate (LAL) assay. P-beads bound LPS dissolved in solutions of ethanol, pH4, pH10, and 0.5M NaCl and LPS bound to the P-beads was quantitatively assayed. The sensitivity of this assay was observed to be approximately 0.1pg/mL LPS. P-beads bound LPS dissolved in antithrombin III (AT III) solution which is a strong inhibitor of activated factors C and B as well as the clotting enzyme in the LAL assay; the inhibitory effect of AT III was completely reversed upon washing the P-beads with 25% acetonitrile. This was employed as the first step for the detection of free LPS in plasma using the LAL assay. LPS added to human plasma at 0°C followed by application to the P-beads and subsequent washing with 25% acetonitrile resulted in low LPS activity as detected by the LAL assay. However, further washing of the P-beads with 0.1% Triton X100 in 25% acetonitrile resulted in high LPS activity. This is the first instance of quantitative detection of free LPS in plasma using the LAL assay, and the sensitivity of this method was observed to be 1pg/mL of LPS. The proteins eluted in the 0.1% Triton X-100 wash were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Two protein bands of 28kDa and 18kDa were predominantly observed. Mass spectrometry analysis revealed that the 28kDa and 18kDa bands corresponded to apolipoprotein A-I (apoA-I) and apolipoprotein A-II (apoA-II), respectively. ApoA-I and apoA-II are components of high density lipoprotein (HDL). Thus, it is likely that the P-beads-bound LPS was sequestered by HDL, resulting in neutralization of its toxicity. This study showed that by using P-beads, free LPS in plasma can be quantitatively measured by the LAL assay at a concentration of 1pg/mL.
- Published
- 2014
36. Examination of cervical neurinomas of the brachial plexus
- Author
-
Yojiro Kawamura, Sei Kobayashi, Hiroshi Gomibuchi, Go Takahashi, Yoichi Ikenoya, Toshikazu Shimane, Naokazu Fujii, Taisuke Nakamura, Tomoaki Mori, and Yuko Shimotatara
- Subjects
business.industry ,Medicine ,Anatomy ,business ,Brachial plexus - Published
- 2014
37. A Study on Concurrent Chemoradiation Therapy in Elderly Patients with Head and Neck Cancer
- Author
-
Toshikazu Shimane, Yuko Shimotatara, Kenichiro Ikeda, Kenichiro Kawaguchi, Sei Kobayashi, Aya Watanabe, Hiroshi Gomibuchi, Naokazu Fujii, and Taisuke Nakamura
- Subjects
Oncology ,chemistry.chemical_compound ,medicine.medical_specialty ,chemistry ,Otorhinolaryngology ,business.industry ,Internal medicine ,Head and neck cancer ,medicine ,Nedaplatin ,Concurrent chemoradiation ,medicine.disease ,business - Published
- 2014
38. Chapter 13 The Pivotal Role of Cholesterol and Membrane Lipid Rafts in the Ca
- Author
-
Ying Zhang, Hiroko Kishi, Katsuko Kajiya, Tomoka Morita, and Sei Kobayashi
- Subjects
Shear (geology) ,Human muscle ,Chemistry ,medicine ,Stiffness ,Supersonic speed ,Composite material ,medicine.symptom - Published
- 2016
39. Influence of mesenchymal stem cells on stomach tissue engineering using small intestinal submucosa
- Author
-
Tomio Ueno, Masaaki Oka, Atsunori Oga, Sei Kobayashi, Hiroki Nakatsu, Taku Nishimura, and Mitsuhiro Nakao
- Subjects
Pathology ,medicine.medical_specialty ,extracellular matrix ,Biomedical Engineering ,Medicine (miscellaneous) ,Regenerative Medicine ,GFP ,Biomaterials ,Muscular layer ,Intestinal mucosa ,Tissue engineering ,medicine ,Animals ,small intestinal submucosa ,Intestinal Mucosa ,Research Articles ,mesenchymal stem cell ,Tissue Engineering ,Chemistry ,Stomach ,Regeneration (biology) ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Rats ,SIS ,medicine.anatomical_structure ,stomach tissue engineering ,Gastric Mucosa ,Female ,Bone marrow ,Ex vivo - Abstract
Small intestinal submucosa (SIS) is a biodegradable collagen-rich matrix containing functional growth factors. We have previously reported encouraging outcomes for regeneration of an artificial defect in the rodent stomach using SIS grafts, although the muscular layer was diminutive. In this study, we investigated the feasibility of SIS in conjunction with mesenchymal stem cells (MSCs) for regeneration of the gastrointestinal tract. MSCs from the bone marrow of green fluorescence protein (GFP)-transgenic Sprague–Dawley (SD) rats were isolated and expanded ex vivo. A 1 cm whole-layer stomach defect in SD rats was repaired using: a plain SIS graft without MSCs (group 1, control); a plain SIS graft followed by intravenous injection of MSCs (group 2); a SIS graft co-cultured with MSCs (group 3); or a SIS sandwich containing an MSC sheet (group 4). Pharmacological, electrophysiological and immunohistochemical examination was performed to evaluate the regenerated stomach tissue. Contractility in response to a muscarinic receptor agonist, a nitric oxide precursor or electrical field stimulation was observed in all groups. SIS grafts seeded with MSCs (groups 3 and 4) appeared to support improved regeneration compared with SIS grafts not seeded with MSCs (groups 1 and 2), by enabling the development of well-structured smooth muscle layers of significantly increased length. GFP expression was detected in the regenerated interstitial tissue, with fibroblast-like cells in the seeded-SIS groups. SIS potently induced pharmacological and electrophysiological regeneration of the digestive tract, and seeded MSCs provided an enriched environment that supported tissue regeneration by the SIS graft in the engineered stomach. © 2013 The Authors. Journal of Tissue Engineering and Regenerative Medicine published by John Wiley & Sons, Ltd.
- Published
- 2013
40. Clinical study of salvage surgery after concurrent chemoradiotherapy
- Author
-
Go Takahashi, Toshikazu Shimane, Hiroshi Gomibuchi, Yojiro Kawamura, Yoichi Ikenoya, Naokazu Fujii, Taisuke Nakamura, Yuko Shimotatara, Tomoaki Mori, and Sei Kobayashi
- Subjects
Clinical study ,medicine.medical_specialty ,Oncology ,Otorhinolaryngology ,business.industry ,medicine ,Salvage surgery ,business ,Surgery ,Concurrent chemoradiotherapy - Published
- 2013
41. A case of neurinoma of the superior laryngeal nerve
- Author
-
Sei Kobayashi, Takatoshi Tokudome, Chika Ogura, Hiroshi Gomibuchi, Naokazu Fujii, Taisuke Nakamura, Takeyuki Sanbe, Yuko Shimotatara, Toshikazu Shimane, and Kenichiro Kawaguchi
- Subjects
Superior laryngeal nerve ,medicine.medical_specialty ,business.industry ,Medicine ,business ,Surgery - Published
- 2013
42. A study of operations on cases with cervical neurinomas
- Author
-
Sei Kobayashi, Yojiro Kawamura, Toshikazu Shimane, Rio Akiyama, Taisuke Nakamura, Hiroshi Gomibuchi, Tomoaki Mori, Yuko Shimotatara, and Yoichi Ikenoya
- Subjects
medicine.medical_specialty ,business.industry ,General surgery ,Medicine ,business - Published
- 2013
43. Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca
- Author
-
Ying, Zhang, Min, Zhang, Bochao, Lyu, Hiroko, Kishi, and Sei, Kobayashi
- Subjects
rho-Associated Kinases ,Myosin Light Chains ,Phosphorylcholine ,fungi ,Cell Membrane ,Coronary Vessels ,Muscle, Smooth, Vascular ,Article ,Protein Transport ,Sphingosine ,Fatty Acids, Omega-6 ,Fatty Acids, Omega-3 ,Fatty Acids, Unsaturated ,Humans ,Calcium ,Phosphorylation ,Cells, Cultured ,Muscle Contraction - Abstract
We previously reported that eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid (n-3 PUFA), effectively inhibits sphingosylphosphorylcholine (SPC)-induced Ca2+-sensitization of vascular smooth muscle (VSM) contraction which is a major cause of cardiovascular and cerebrovascular vasospasm, and EPA is utilized clinically to prevent cerebrovascular vasospasm. In this study, we clearly demonstrate that docosapentaenoic acid (DPA), which exists in two forms as omega-3 (n-3) and omega-6 (n-6) PUFA, strongly inhibits SPC-induced contraction in VSM tissue and human coronary artery smooth muscle cells (CASMCs), with little effect on Ca2+-dependent contraction. Furthermore, n-3 and n-6 DPA inhibited the activation and translocation of Rho-kinase from cytosol to cell membrane. Additionally, SPC-induced phosphorylation of myosin light chain (MLC) was inhibited in n-3 and n-6 DPA pretreated smooth muscleVSM cells and tissues. In summary, we provide direct evidence that n-3 and n-6 DPA effectively equally inhibits SPC-induced contraction by inhibiting Rho-kinase activation and translocation to the cell membrane.
- Published
- 2016
44. A Case of Solitary Thyroid Metastasis from Kidney Cancer
- Author
-
Kenichiro Ikeda, Toshikazu Shimane, Takeyuki Sanbe, Sei Kobayashi, Takeshi Hayashi, Akane Sugimoto, Aya Watanabe, Hiroshi Gomibuchi, and Rio Akiyama
- Subjects
Oncology ,medicine.medical_specialty ,Pathology ,Otorhinolaryngology ,Renal cell carcinoma ,Fine needle aspiration cytology ,Thyroid metastasis ,business.industry ,Internal medicine ,medicine ,medicine.disease ,business ,Kidney cancer - Published
- 2012
45. A Case of Angiosarcoma Originating in the Internal Jugular Vein
- Author
-
Akane Sugimoto, Takeyuki Sanbe, Toshikazu Shimane, Takeshi Hayashi, Aya Uzuki, Sei Kobayashi, Harumi Suzaki, Kenichiro Ikeda, and Rio Akiyama
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Neck dissection ,digestive system diseases ,Radiation therapy ,medicine.anatomical_structure ,Otorhinolaryngology ,Scalp ,cardiovascular system ,medicine ,Angiosarcoma ,Radiology ,business ,neoplasms ,Internal jugular vein - Abstract
Angiosarcoma is a rare but highly aggressive tumor tending to occur on the scalp and face in elderly subjects. Some reports suggest that trauma, neck dissection, and radiotherapy may induce it. We report a case of angiosarcoma originating in the internal jugular vein of a young man.
- Published
- 2012
46. Nasopharyngeal cancer presented with attacks of syncope
- Author
-
Rio Akiyama, Hiroshi Gomibuti, Aya Uzuki, Toshikazu Shimane, Sei Kobayashi, Takeyuki Sanbe, Kenitirou Ikeda, and Takeshi Hayashi
- Subjects
medicine.medical_specialty ,biology ,business.industry ,Anesthesia ,Syncope (genus) ,Medicine ,business ,biology.organism_classification ,Surgery ,Nasopharyngeal cancer - Abstract
頭頸部癌は頸部リンパ節転移が多く認められ,この転移巣による物理的な頸動脈圧迫で失神発作(頸動脈洞過敏症候群:Carotid Sinus Hypersensitivity Syndrome以下CSHS)を起こすことは報告されているが,ほとんどは末期癌患者であり初発症状として失神発作を起こした症例は少ない。今回,われわれは頻回の失神発作を初発症状とした,上咽頭癌の頸部リンパ節転移の症例を経験した。術前にペースメーカーを挿入していたため,手術治療を選択し頸部郭清術を施行したところ失神発作は消失した。CSHSは,頸動脈反射が何らかの原因で過敏反応を示す場合をいう。頸部リンパ節転移が原因のCSHSの治療には,手術・化学放射線同時併用療法・薬物療法などが挙げられる。頸部リンパ節転移を認める症例は進行癌が多いため,治療の選択肢を広げるためにもペースメーカー挿入を積極的に行うべきであると考えられた。
- Published
- 2012
47. A Case of Primary Paranasal Sinus Rhabdomyosarcomas in an Adult
- Author
-
Kenichiro Ikeda, Rio Akiyama, Takeyuki Sanbe, Toshikazu Shimane, Aya Uzuki, Akane Sugimoto, and Sei Kobayashi
- Subjects
medicine.medical_specialty ,medicine.anatomical_structure ,Otorhinolaryngology ,business.industry ,Medicine ,business ,Rhabdomyosarcoma ,medicine.disease ,Sinus (anatomy) ,Surgery - Published
- 2012
48. Synchronous phosphorylation of CPI-17 and MYPT1 is essential for inducing Ca2+ sensitization in intestinal smooth muscle
- Author
-
D. Mori, Takahisa Murata, Masatoshi Hori, H. Kishi, Sei Kobayashi, Takashi Ohama, and Hiroshi Ozaki
- Subjects
medicine.medical_specialty ,Carbachol ,Contraction (grammar) ,Endocrine and Autonomic Systems ,Physiology ,Kinase ,Gastroenterology ,Biology ,medicine.anatomical_structure ,Endocrinology ,Myosin phosphatase activity ,Internal medicine ,medicine ,Phosphorylation ,medicine.symptom ,Protein kinase C ,Sensitization ,medicine.drug ,Muscle contraction - Abstract
Background Myosin phosphatase activity is regulated by mechanisms involving the phosphorylation of CPI-17 and MYPT1, primarily based on studies with tonic-type vascular smooth muscles. This study examined how these mechanisms contribute to the regulation of contraction of a phasic-type intestinal smooth muscle. Methods Phosphorylation levels, tension, and Ca2+ sensitization was detected in rat ileal smooth muscle. Key Results In rat ileal smooth muscle, phosphorylation level of CPI-17 at Thr38 and MYPT1 at Thr853, but not MYPT1 at Thr696, were increased with carbachol (1 μmol L−1) accompanied with muscle contraction. The PKC inhibitor Go6976 (1 μmol L−1) inhibited the carbachol-induced phosphorylation of CPI-17, whereas the Rho-associated kinase (ROCK) inhibitor, Y-27632 (10 μmol L−1) inhibited the carbachol-induced phosphorylation of both CPI-17 and MYPT1. Application of Go6976 or Y-27632 alone inhibited the carbachol-induced contraction; however, the combined application of these inhibitors did not inhibit the contraction in an additive manner. In β-escin-permeabilized ileal strip, treatment with antiphosphorylated antibodies for CPI-17 at Thr38 and MYPT1 at Thr853 and Thr696 alone almost completely abolished the Ca2+ sensitization due to carbachol with GTP. Conclusions & Inferences In conclusion, receptor stimulation increases the Ca2+ sensitivity of contractile elements through CPI-17 phosphorylation via the PKC/ROCK pathways and MYPT1 phosphorylation via the ROCK pathway, when these mechanisms operate cooperatively and/or synchronously in intestinal smooth muscle.
- Published
- 2011
49. A Case of Extra Abdominal Desmoid on the Neck
- Author
-
Kenichiro Ikeda, Harumi Suzaki, Tomoaki Mori, Tomohiro Ono, Shunya Egawa, Toshikazu Shimane, Sei Kobayashi, and Takeyuki Sanbe
- Subjects
medicine.medical_specialty ,Solitary fibrous tumor ,Extra abdominal ,business.industry ,medicine.medical_treatment ,Schwannoma ,medicine.disease ,Infiltrative Growth ,body regions ,Radiation therapy ,Leiomyoma ,Otorhinolaryngology ,medicine ,Radiology ,business ,Head and neck ,Gy Radiation - Abstract
Desmoids, although pathologically benign, are called “locally malignant tumors”, due to their infiltrative growth and tendency to recur locally. They are roughly classified as abdominal (90%) or extra-abdominal (10%), with 12-20% of extra abdominal occurring on the head and neck. We report a case of extra abdominal frontal neck desmoid. A 47-year-old woman was seen for a frontal neck. Unable to pathologically diagnose the tumor preoperatively, we suspected a schwannoma, leiomyoma, or solitary fibrous tumor. After two total resections and immediate local recurrence, we suggested that she undergo neck radiotherapy. No recurrence has occurred in the 6 months since postoperative 50 Gy radiation. Neck tumor occurrence should thus suggest the possibility of desmoids.
- Published
- 2011
50. Investigation of malignant thyroid tumors
- Author
-
Toshikazu Shimane, Hiroshi Gomibuchi, Sei Kobayashi, Aya Uzuki, Takeshi Hayashi, Rio Akiyama, Takeyuki Sanbe, Akane Sugimoto, and Yoichi Ikenoya
- Subjects
medicine.medical_specialty ,Oncology ,Otorhinolaryngology ,business.industry ,Urology ,Medicine ,business ,Thyroid tumors - Abstract
当科にて甲状腺悪性腫瘍の診断で一次治療を施行した80例について検討した。期間は1995年1月から2010年6月までであったが,主訴や性別,年齢,穿刺吸引細胞診,病理組織学的分類,TNM分類など今回の検討内容が確認できなかった症例は除外した。男女比は1:2.6で女性に多く,病理組織学的分類は乳頭癌が71例(88%)を占め,濾胞癌6例(8%),未分化癌1例(1%)で髄様癌は認めなかった。また悪性リンパ腫を2例(3%)認めた。穿刺吸引細胞診の敏感度は90.3%であった。手術術式は葉切除が44%と多く,全摘33%,亜全摘19%,全摘+気管合併切除3%,全摘+喉頭全摘出術1%と続いた。平均経過観察期間が39.9ヶ月と短期間であるが,未分化癌の1例を除いて全例生存している。
- Published
- 2011
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