1. Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories
- Author
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Bernal, Paulina Nuñez, Bouwmeester, Manon, Madrid-Wolff, Jorge, Falandt, Marc, Florczak, Sammy, Rodriguez, Nuria Ginés, Li, Yang, Größbacher, Gabriel, Samsom, Roos-Anne, van Wolferen, Monique, van der Laan, Luc, Delrot, Paul, Loterie, Damien, Malda, Jos, Moser, Christophe, Spee, Bart, Levato, Riccardo, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, Afd Pharmacology, dES RMSC, CS_STEM, CS_Locomotion, Surgery, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, Afd Pharmacology, dES RMSC, CS_STEM, and CS_Locomotion
- Subjects
disease ,Tissue Engineering ,Tissue Scaffolds ,biofabrication ,Mechanical Engineering ,Bioprinting ,Regenerative ,spheroids ,Hydrogels ,bioink ,culture ,bioresins ,Organoids ,Materials Science(all) ,Liver ,SDG 3 - Good Health and Well-being ,volumetric additive manufacturing ,Mechanics of Materials ,Printing, Three-Dimensional ,Gelatin ,Humans ,General Materials Science ,protein ,hydrogels ,light-based 3D printing - Abstract
Organ- and tissue-level biological functions are intimately linked to micro- scale cell–cell interactions and to the overarching tissue architecture. Together, biofabrication and organoid technologies offer the unique potential to engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces the volumetric bioprinting of complex organoid-laden constructs, which capture key functions of the human liver. Volumetric bioprinting via optical tomography shapes organoid- laden gelatin hydrogels into complex centimeter-scale 3D structures in under 20 s. Optically tuned bioresins enable refractive index matching of specific intracellular structures, countering the disruptive impact of cell-mediated light scattering on printing resolution. This layerless, nozzle-free technique poses no harmful mechanical stresses on organoids, resulting in superior viability and morphology preservation post-printing. Bioprinted organoids undergo hepatocytic differentiation showing albumin synthesis, liver-specific enzyme activity, and remarkably acquired native-like polarization. Organoids embedded within low stiffness gelatins (
- Published
- 2022