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Aims: The aim of the paper was to isolate and characterize the promoters of two cucumber TCTP genes (CsTCTP1 and CsTCTP2) and evaluate their active domains. Study Design: CsTCTP1 and CsTCTP2 promoter activity were analyzed under treatments with different exogenous hormones. Place and Duration of Study: In 2017, these experiments were conducted in College of Bioscience and Biotechnology of Shenyang Agricultural University (Lab 240). Methodology: CsTCTPpro::GUS constructs were generated by using double digests method. Transient expression was mediated by Agrobacterium tumefaciens GV3101. Histochemical and Fluorometric GUS Assays were follow by the biochemical method. Results: Bioinformatics analysis revealed some hormone- and defense-related response elements. Histochemical and fluorometric GUS assays demonstrated that the 0.7-kb CsTCTP1 promoter (proT1-0.7kb) and 0.7-kb and 1.3-kb CsTCTP2 promoters (proT2-0.7kb and proT2-1.3kb) had strong transcriptional activity. In addition, we used exogenous hormones (abscisic acid [ABA], salicylic acid [SA], and ethylene [ETH]) for treatment. The results showed that proT1-0.7kb and proT2-1.3kb activity were upregulated in the ABA treatment group, suggesting that these promoter sequences may contain ABA-related response elements. However, in the SA and ETH treatment groups, the activity of all the promoter fragments of CsTCTP1 and CsTCTP2 declined to different degrees, suggesting that SA and ETH may have negative regulatory effects on CsTCTP1 and CsTCTP2 promoters. Conclusion: Taken together, these results suggest that the proT1-0.7kb promoter of CsTCTP1 and proT2-1.3kb promoter of CsTCTP2 may contain ABA-related response elements, and SA and ETH may have negative regulatory effects on the CsTCTP1 and CsTCTP2 promoters. This study will help to further understand the expression patterns and the regulatory mechanism of gene transcriptional regulation.

OBJECTIVE To describe Chinese Lynch syndrome-associated colorectal cancer patients' self-concept and adherence to surveillance and explore the factors influencing patients' adherence to surveillance. METHODS This was a cross-sectional quantitative study of a group of 83 Lynch syndrome-associated colorectal cancer patients, using the Lynch syndrome self-concept scale and medical records. IBM SPSS 20.0 was used for statistical analysis. RESULTS Patients' adherence to surveillance, for colorectal cancer, gastric cancer and small intestinal cancer, endometrial and ovarian cancer, they were 93.2%, 41.8% and 56.7% respectively. Older patients had lower surveillance adherence of colorectal cancer, gastric cancer and small intestinal cancer than younger patients (p

Three biochars from wheat straw(WSC), chicken manure(CMC) and rice husk(RHC) were prepared as persulfate(PS) activators for p-nitropheol(PNP) removal. RHC exhibitted the best adsorption performance, followed by WSC and CMC, which was consistant with the surface area value. PS addition further promoted the PNP removal, indicating that a synergistic effect existed in the biochar/PS combined system. The composition and textual properties of biochar had a significant effect on the reactivity of activator and the function groups containing oxgygen or nitrogen might play important roles in the reaction. WSC could perform efficiently over a pH range from 4.4 to 10.4. The decrease in activation performance in cycle experiments was possiblely related to the loss of oxgygen-containing groups.

Acute kidney injury (AKI) in cancer patients may disrupt anticarcinogenic treatment and greatly increase associated mortality rates. The present study reported on the management of cancer patients with AKI and, from the nephrologic viewpoint, on the significance of fine volume control during the continuous renal replacement therapy (CRRT). The records of 117 cancer patients with AKI treated over three years were reviewed and their data were compared with those of 120 healthy controls. AKI was defined according to the Kidney Disease: Improving Global Outcomes criteria with serum creatinine levels determined on initial admission and for the diagnosis of AKI. CRRT with concomitant impedance cardiography (ICG) monitoring was performed in 79 patients. On average, AKI manifested as a 1.68±0.38-fold increase in serum creatinine within 10.2±5.7 days. The causes of AKI were diverse, with the major ones being nephrotoxic agents, hypotension and obstructive nephropathy. Renal biopsy confirmed two cases of thrombotic microangiopathy, due to the use of interfon-α and sunitinib malate, respectively, and a third case of cast nephropathy caused by immunoglobulin D multiple myeloma. The patients were generally marantic and had compromised cardiac function compared with the healthy controls. The CRRT prescription was discriminatingly optimized by the ICG parameters effecting discreet fluid balance, as the thoracic fluid content was significantly correlated with the ultrafiltration rate. By considering the causative mechanisms and applying subtle ICG-assisted volume control, the present study may thus help to improve the safety and efficacy of CRRT in cancer patients with AKI. In addition, it provided information to bring advances in onconephrology, a novel nephrological subspecialty field.

Ion-pair reversed phase-high performance liquid chromatography (RP-HPLC) with evaporative light scattering detection (ELSD) has been established for the analysis of fosetyl-aluminium and relevant impurities in technical or formulated substance. The method was achieved on a Symmetry Shield RP18 column by using n-butylamine as ion-pairing agent. The mobile phase was methanol-redistilled water containing 0.5% n-butylamine which was adjusted pH 5.0 with acetic acid (8:92, v/v) and the flow rate was set at 0.8 mL/min. The active ingredient fosetyl-aluminium was successfully separated from relevant impurities under the conditions. The calibration curve was linear in the range of 100-1200 mg/L. The average recoveries of two fortification levels (100 mg/L and 1000 mg/L) were 100.58% and 99.53%, and the relative standard deviations (RSDs) were 0.62% and 0.49%, respectively. The method is rapid, simple and accurate which provides another new and reliable means for the analysis of fosetyl-aluminium in its technical or formulated substance.

To increase the viscosity of chitosan/glycerol phosphate (C/GP) and to improve its preparation technique in order to develop the application range of C/GP.Chitosan was treated by high-pressure vapor sterilization in order to prepare high viscous C/GP (HV-C/GP) and prepare C/GP by standard methods. The rheologic changes of HV-C/GP and C/GP were detected dynamically by the Gemini rheometer. The initial solution viscosity, gelation temperature and gelation time were evaluated after the viscosity of the materials were increased. Two gelation materials were placed into continuous flow thermostated cells under the same condition and harvest them at predetermined time intervals, 1st, 2nd, 5th, 10th and 25th days, then they were dried, weighed and the mass loss rate was calculated. Ultrastructure of the freeze-dried samples was visualized by the scanning electron microscope.The initial viscosity of C/GP was 1.81 Pas and that of HV-C/GP was 17.24 Pas. The latter one increased 10 times as well as the former one. The gelation temperature of C/GP was 37 degrees C and that of HV-C/GP was 34 degrees C. There was no remarkable difference in gelation time between them. The mass loss rate of HV-C/GP at first day was 72.5% and at 25th days was 90.8%, while that of C/GP was 55.4% and 78.2%. Porous network structure was observed by the scanning electron microscope in both of them. The pore diameter of C/GP was 50-100 microm and that of HV-C/GP was 30-50 microm, which was obviously smaller than the former.The viscosity of HV-C/GP prepared by improved technique obviously increases and the thermosensitivity has no significant changes. The degradation time of HV-C/GP in vitro lengthens. The micrographs show that the HV-C/GP gels are porous and the pore diameter are smaller than C/GP.