139 results on '"Ruifang Wu"'
Search Results
2. Deficiency of p53 Causes the Inadequate Expression of miR-1246 in B Cells of Systemic Lupus Erythematosus
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Qing Zhang, Yu Liu, Jieyue Liao, Ruifang Wu, Yi Zhan, Peng Zhang, and Shuangyan Luo
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Histones ,B-Lymphocytes ,MicroRNAs ,Immunoglobulin G ,Immunology ,Humans ,Lupus Erythematosus, Systemic ,Immunology and Allergy ,Clinical and Human Immunology ,Tumor Suppressor Protein p53 ,CREB-Binding Protein - Abstract
Underexpression of p53 is considered the leading cause of the decreased miR-1246 expression in B cells of systemic lupus erythematosus (SLE) patients, yet the exact mechanism of action still remains unclear. To further explore the molecular mechanism of p53 upregulating miR-1246 expression, we targeted the methylation and acetylation of histone H3 in the miR-1246 promoter region of SLE B cells. We found that increased histone H3 trimethylation at Lys27 (H3K27me3) and decreased histone H3 acetylation at Lys9 and Lys14 (H3K9/K14ac) in the miR-1246 promoter region are essential for the low expression of miR-1246 in SLE B cells. p53 can promote miR-1246 transcription by recruiting Jumonji domain–containing protein 3 (JMJD3), E1A-binding protein p300 (EP300), and CREB-binding protein (CBP) to bind to the miR-1246 promoter, downregulating H3K27me3 and upregulating H3K9/K14ac. Furthermore, early B cell factor 1 (EBF1), CD40, CD38, and X box binding protein-1 (XBP-1) expression levels in SLE B cells transfected with p53 expression plasmid were significantly decreased, whereas autoantibody IgG production in autologous CD4+ T cells cocultured with overexpressed p53 SLE B cells was reduced. Collectively, our data suggest that the reduction of p53 decreases miR-1246 expression via upregulation of H3K27me3 and downregulation of H3K9/14ac, which in turn results in SLE B cell hyperactivity.
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- 2022
3. DNA methylation markers in peripheral blood for psoriatic arthritis
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Min, Deng, Yuwen, Su, Ruifang, Wu, Siying, Li, Yanshan, Zhu, Guishao, Tang, Xiaoli, Shi, Tian, Zhou, Ming, Zhao, and Qianjin, Lu
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Dermatology ,Molecular Biology ,Biochemistry - Abstract
The clinical manifestations of psoriatic arthritis (PsA) are highly heterogeneous and no reliable diagnostic biomarkers exist.We explored the role of DNA methylation CpG markers in the diagnosis of PsA.DNA methylation array was used to screen for differentially methylated sites (DMSs) in the discovery phase (PsA, n = 25; healthy controls [HCs], n = 19; psoriasis vulgaris [PsV], n = 20). In the validation phase, pyrosequencing was used to identify the DMSs in an expanded cohort (PsA, n = 60; HCs, n = 91; PsV, n = 48; rheumatoid arthritis [RA], n = 60). Logistic regression prediction models were established based on the identified DMSs for the diagnosis of PsA.A total of 17 DMSs differentiating PsA and HCs as well as 11 DMSs differentiating PsA and PsV were screened in the discovery phase. A total of six DMSs (chr14: cg07940072, chr14: 38061320, chr9: cg15734589, chr6: cg12800266, chr3: cg12992827, chr6: cg24500972) differentiating PsA and HCs and two DMSs (chr12: cg16459382, chr2: cg16348668) differentiating PsA and PsV were identified using pyrosequencing. Three logistic regression prediction models were established based on the identified DMSs, which distinguished PsA, RA, PsV, and HCs (P 0.001). The models performed well in differentiating PsA from HCs, RA, and PsV (AUC: 0.858, 0.851, and 0.976, respectively).The models based on methylated CpG sites are useful for distinguishing patients with PsA from HCs and those with RA or PsV and are a highly sensitive and specific diagnostic biomarker for PsA.
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- 2022
4. Effect of acid treatment on the catalytic performance of CuO/Cryptomelane catalyst for CO preferential oxidation in H2-rich streams
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Hui Dang, Wanjun Zhao, Ruifang Wu, Lijun Yue, Yongzhao Wang, Zhenxing Ren, and Yongxiang Zhao
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Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,Condensed Matter Physics - Published
- 2023
5. Effect of Sperm 26S Proteasome on Embryo Formation and Development in In Vitro Fertilization
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Chenglu Wang, Fangyu Wang, Min Zheng, Ruifang Wu, Tianyun Yang, Xiaopan Chen, and Jing Shu
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Adult ,Male ,Proteasome Endopeptidase Complex ,Fertilization ,Sperm Motility ,Humans ,Obstetrics and Gynecology ,Fertilization in Vitro ,Spermatozoa - Abstract
The aim of this study was to evaluate the effects of sperm proteasome activity on fertilization outcome and embryo development after IVF. Following density gradient centrifugation for IVF purpose, the spermatozoa of 84 infertile patients with tubal factor were evaluated by luciferase enzymatic activity to assess the proteasome quantity. The mean age of patients was 33.8 years, and the mean concentration of human spermatozoa 26S proteasome was 674.53 ng/ml. After IVF, the embryos were scored for morphology. The spermatozoa proteasome activity was both positively correlated with fertilization rate in vitro (P = 0.0003) and 2PN rate (P = 0.0007). Compared to low fertilization rate group, the high fertilization rate group showed a significantly higher level of spermatozoa proteasome activity (P = 0.002). In conclusion, sperm proteasome activity provides additional data on sperm functional capacity in terms of fertilization during IVF.
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- 2022
6. Hydrogenation of 2-Hydroxytetrahydrofuran to 1,4-Butanediol Over Ni–Fe/SiO2 Bimetallic Catalysts
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Liping Zhao, Changzhen Wang, Juan Bai, Ruifang Wu, Haitao Li, and Yongxiang Zhao
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General Chemistry ,Catalysis - Published
- 2023
7. First passage time and mean exit time for switching Brownian motion
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Jinying Tong, Ruifang Wu, Qianqian Zhang, Zhenzhong Zhang, and Enwen Zhu
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Modeling and Simulation - Abstract
In this paper, we consider some properties of switching Brownian motion. Combining the analytic method and probabilistic method, some explicit expressions of density functions, the mean exit time and Laplace transform of exit time are given. This paper reveals how drift coefficients impact the first passage probabilities, scale functions and the mean exit time for switching Brownian motion.
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- 2023
8. Identification and characterization of novel compound heterozygous variants in FSHR causing primary ovarian insufficiency with resistant ovary syndrome
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Xiaopan Chen, Linjie Chen, Yang Wang, Chongyi Shu, Yier Zhou, Ruifang Wu, Bihui Jin, Leixiang Yang, Junhui Sun, Ming Qi, and Jing Shu
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Endocrinology, Diabetes and Metabolism - Abstract
Primary ovarian insufficiency (POI) is among the foremost causes of women infertility due to premature partial or total loss of ovarian function. Resistant ovary syndrome (ROS) is a subtype of POI manifested as normal ovarian reserve but insensitive to gonadotropin stimulation. Inactivating variants of follicle-stimulating hormone receptor (FSHR), a class A G-protein coupled receptor, have been associated with POI and are inherited via an autosomal recessive pattern. In this study, we investigated the genetic causes of a primary infertility patient manifested as POI with ROS, and elucidated the structural and functional impact of variants of uncertain significance. Next-generation sequencing (NGS) combined with Sanger sequencing revealed novel compound heterozygous FSHR variants: c.1384G>C/p.Ala462Pro and c.1862C>T/p.Ala621Val, inherited from her father and mother, respectively. The two altered amino acid sequences, localized in the third and seventh transmembrane helix of FSHR, were predicted as deleterious by in silico prediction. In vitro experiments revealed that the p.Ala462Pro variant resulted in barely detectable levels of intracellular signaling both in cAMP-dependent CRE-reporter activity and ERK activation and displayed a severely reduced plasma membrane receptor expression. In contrast, the p.Ala621Val variant resulted in partial loss of receptor activation without disruption of cell surface expression. In conclusion, two unreported inactivating FSHR variants potentially responsible for POI with ROS were first identified. This study expands the current phenotypic and genotypic spectrum of POI.
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- 2023
9. Waste Eggshell to Valuable Co3o4/Eggshell Material as an Efficient Catalyst for Catalytic Decomposition of N2o Under Simulated Real Tail Gases
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Xiaobo Hu, Linyan Feng, Ruifang Wu, Yongzhao Wang, and Yongxiang Zhao
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- 2023
10. Experimental study on the vitrification and xenotransplantation of human ovarian tissue
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Jinghui Zhao, Yuping Ge, Jingyi Li, Yucui Zeng, Guoyi Dong, Liping Zeng, Juanjuan Dai, Hui Du, Mumin Shao, Xuewen Yu, Huan Li, Fang He, Zhouchun Shang, Chun Wang, Gábor Vajta, Huiru Tang, Ruifang Wu, and Yutao Du
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- 2022
11. Enhancement of Cu+ stability under a reducing atmosphere by the long-range electromagnetic effect of Au
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Xin Huang, Haitao Li, Yin Zhang, Ruifang Wu, Lijun Ban, Lin Xi, Zhifang Yin, Jian Peng, Yongxiang Zhao, and Li Fang
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General Materials Science - Abstract
In conventional thermocatalytic reactions under a reducing atmosphere, stabilization of the active Cu+ component and inhibition of over-reduction into metallic Cu0 are extremely challenging.
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- 2022
12. Decreased microRNA‐126 expression in psoriatic CD4 + T cells promotes T‐helper 17 cell differentiation and the formation of dermatitis in imiquimod‐induced psoriasis‐like mice
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Ruifang Wu, Xin Li, Siying Li, Guishao Tang, Suhan Zhang, Yanshan Zhu, Xiaochao Zhang, Min Deng, Siqi Tan, Shuangyan Luo, Qing Zhang, Ming Zhao, Peng Zhang, and Yuwen Su
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Dermatology ,General Medicine - Published
- 2021
13. Effect of Pore Structures on 1,4-Butynediol Hydrogenation over Mesoporous Ni/Al2O3-SiO2 Catalysts
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Chengyan Jiang, Ruifang Wu, Gaoju Yang, Tiancun Xiao, Yongxiang Zhao, Juan Bai, and Changzhen Wang
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chemistry.chemical_compound ,Materials science ,chemistry ,Chemical engineering ,General Chemical Engineering ,1,4-Butynediol ,General Chemistry ,Mesoporous material ,Industrial and Manufacturing Engineering ,Catalysis - Published
- 2021
14. The prevalence of HR-HPV infection based on self-sampling among women in China exhibited some unique epidemiologic features
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Jerome L. Belinson, Xinfeng Qu, Ruifang Wu, Hongxue Luo, Hui Du, and Chun Wang
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Adult ,China ,medicine.medical_specialty ,Genotype ,Epidemiology ,Uterine Cervical Neoplasms ,Cervical intraepithelial neoplasia ,Sampling Studies ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Prevalence ,Humans ,Medicine ,Pooled data ,030212 general & internal medicine ,Human papillomavirus ,Papillomaviridae ,Hpv types ,business.industry ,Screening Trial ,Papillomavirus Infections ,Genetic Variation ,virus diseases ,Middle Aged ,medicine.disease ,female genital diseases and pregnancy complications ,Population Surveillance ,Female ,Self Report ,business ,030217 neurology & neurosurgery ,Self sampling - Abstract
Objective To investigate the epidemiological characteristics of high-risk human papillomavirus(HR-HPV) infection based on vaginal self-collected samples. Study Design and Setting The pooled data of 3045 self-collected samples used for the analysis derived from four previous studies on cervical cancer screening(The Chinese Multi-Center Screening Trial, CHIMUST; The Shenzhen Cervical Cancer Screening Trial-2, SHENCCAST-2; The Chinese Cervical Cancer Prevention Study, CHIPCAPS; Pingshan trial, PINGSHAN)conducted across China by our team since 2011. These cases were evaluated for HR-HPV type prevalence relative to lesion grade and age. The occurrence of cervical intraepithelial neoplasia(CIN) with specific HPV types and the influence of co-infection is explored. Results The top three most common genotypes among the HR-HPV positives were HPV-52(23.4%), HPV-16(18.0%), and HPV-58(15.50%). For women with CIN2+, the most frequent genotypes were HPV-16, 58, 52, and 18 in sequence. HPV-16 accounted for the majority of CIN2/CIN3/Ca with attribution rate of 23.86%, 44.78% and 50.00% respectively. HPV-58 accounted for 19.48%, 16.79% and 13.46% respectively. CIN2+ was found in the following types most frequently: HPV-16(31.23%), HPV-33(24.03%), HPV-58(18.41%), HPV-31(11.76%), HPV-18(7.75%), and HPV-52(7.30%). HPV-16 showed preference for co-infection with HPV-52 and HPV-58. Conclusion The prevalence of HR-HPV infection based on self-sampling among women in China exhibited some unique epidemiologic features.
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- 2021
15. Effect of Sn addition on the catalytic performance of a Pd–Cu/attapulgite catalyst for room-temperature CO oxidation under moisture-rich conditions
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Ruifang Wu, Hui Dang, Yongxiang Zhao, Yongzhao Wang, Xiao Li, and Wanjun Zhao
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Adsorption ,Materials science ,Water resistance ,Moisture ,Chemical engineering ,Physical and Theoretical Chemistry ,Catalysis ,Water vapor - Abstract
Herein, the effect of SnCl4 addition on the catalytic performance of Pd–Cu/attapulgite (Pd–Cu/APT) synthesized by a deposition–precipitation method has been investigated. Compared with Pd–Cu/APT, the suitable addition of Sn has a conspicuous facilitation on the catalytic performance of Pd–Cu–Sn/APT for CO oxidation. The characterization results reveal that the incorporation of Sn gives rise to the formation of SnO2, which conduces to produce the more Cu2Cl(OH)3 active phases and Cu+ species in Pd–Cu–Sn/APT and establish a mutual interaction between Sn and Pd, Cu species. Accordingly, the Pd0 species generated during CO oxidation over Pd–Cu–Sn/APT can be more easily oxidized than those over Pd–Cu/APT. Additionally, the generation of SnO2 is prone to suppress the adsorption of water vapor on the catalyst surface, hence augmenting the water resistance of Pd–Cu–Sn/APT. Therefore, Sn-modified Pd–Cu/APT catalyst exhibits the more excellent catalytic performance for CO oxidation at room temperature under moisture-rich conditions.
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- 2021
16. Ocular surface disorders affect quality of life in patients with autoimmune blistering skin diseases: a cross-sectional study
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Huanmin, Kang, Mengbo, Wu, Jianing, Feng, Yuerong, Ren, Yingyi, Liu, Wen, Shi, Yingqian, Peng, Yixin, Tan, Ruifang, Wu, Guiying, Zhang, and Yan, He
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Meibomian Glands ,General Medicine ,Skin Diseases ,Autoimmune Diseases ,Ophthalmology ,Cross-Sectional Studies ,Blister ,Corneal Opacity ,Tears ,Surveys and Questionnaires ,Quality of Life ,Eyelid Diseases ,Humans ,Dry Eye Syndromes ,Fluorescein - Abstract
Background Autoimmune blistering skin diseases (AIBD) are a group of rare chronic autoimmune diseases which are associated with ocular surface diseases especially dry eye disease. This study is designed to investigate the relationship between ocular surface disorders and quality of life among patients with autoimmune blistering skin diseases. Methods Twenty-four AIBD patients (18 pemphigus and 7 pemphigoid) and twenty-five non-AIBD controls were included. Ocular surface disease index (OSDI), ocular surface evaluation, including slit-lamp examination, Schirmer I test, tear break-up time, corneal fluorescein staining, lid-parallel conjunctival folds, meibomian gland evaluation, presence of symblepharon and corneal opacity were assessed. Life quality was evaluated by multiple questionnaires, including Medical Outcomes Study 36-Item Short Form Questionnaire (SF-36), Hospital Anxiety and Depression Scale (HADS), Pittsburgh Sleep Quality Index (PSQI) and Health Assessment Questionnaire-Disability Index (HAQ-DI). Ocular surface tests and quality of life were compared between AIBD patients and non-AIBD controls. In the AIBD patients, the associations between ocular surface parameters and quality of life were also evaluated. Results 92% of AIBD patients and 87.5% of age- and sex-matched non-AIBD controls were diagnosed with dry eye in this study. Compared with non-AIBD controls, AIBD patients reported lower SF-36 scores (P P Conclusions AIBD patients experience reduced quality of life and more severe ocular surface disorders including dry eye, symblepharon and corneal opacity. Early treatments of dry eye and collaborations among multidisciplinary physicians are necessary in patients with AIBD.
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- 2022
17. Evaluation of p16INK4a immunocytology and human papillomavirus (HPV) genotyping triage after primary HPV cervical cancer screening on self-samples in China
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Peisha Yan, Hui Du, Fangbin Song, Xinfeng Qu, Xia Huang, Ruifang Wu, Jerome L. Belinson, and Chun Wang
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0301 basic medicine ,Cervical cancer ,Colposcopy ,Oncology ,medicine.medical_specialty ,Cervical screening ,medicine.diagnostic_test ,business.industry ,Obstetrics and Gynecology ,Guideline ,medicine.disease ,Triage ,female genital diseases and pregnancy complications ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Liquid-based cytology ,Internal medicine ,Cohort ,medicine ,business ,Genotyping - Abstract
Objective Self-sampling for human papillomavirus (HPV) testing is an effective option to increase the cervical screening coverage. How to best triage HPV-positive self-samples remains controversial. Here, we evaluated the performance of a novel p16INK4a immunocytology approach (p16) and HPV genotyping in triaging HPV-positive self-samples. Methods A cohort of 73699 women were screened via SeqHPV assay on self-samples. HPV-positive women who met any sequential positive result of HPV16/18 or VIA or p16 were referred for colposcopy. A triage strategy was considered favorable if the NPV for CIN3+ was ≥98%, combined with an improvement of sensitivity and specificity in comparison to the comparator, being the ‘ASC-US+’ triage and the guideline strategy (HPV16/18+ or ASC-US+). Results A total of 3510 HPV-positive women were included, 422 (12.0%) CIN2+ and 247 (7.0%) CIN3+ were identified. The positivity of p16 and ASC-US+ were 36.3% and 22.2%, respectively. p16 was more sensitive and less specific than ASC-US+ (P Conclusions Our findings indicate the promising utility of p16 immunocytology via adjusting the staining score or serving as an ancillary tool to liquid-based cytology or combining with genotyping for the triage of HPV-positive self-samples, which promotes the precise screening of cervical cancer.
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- 2021
18. Importance of Zinc Oxide in Cu-Based Catalysts for the Ethynylation of Formaldehyde to 1,4-Butynediol
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Ruifang Wu, Lijun Ban, Haitao Li, Xin Huang, Jianghong Zhao, Yongxiang Zhao, and Yin Zhang
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Materials science ,Formaldehyde ,Biomass ,chemistry.chemical_element ,Zinc ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Catalysis ,chemistry.chemical_compound ,General Energy ,chemistry ,Acetylene ,1,4-Butynediol ,Physical and Theoretical Chemistry ,Nuclear chemistry - Abstract
As more technologies have been developed to produce formaldehyde and acetylene from biomass, the condensation–addition reaction between formaldehyde and acetylene provides a green and atom-economic...
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- 2021
19. Surface plasmon resonance effect of Ag@BiOCl and its enhanced visible light-photodegradation of acid red B
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Yun-Kai Lv, Ruifang Wu, Jing Wang, Zhengming Yu, Linlin Liang, Hua Chen, and Qi Shi
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Range (particle radiation) ,Materials science ,engineering.material ,Condensed Matter Physics ,Photochemistry ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,engineering ,Photocatalysis ,Degradation (geology) ,Noble metal ,Electrical and Electronic Engineering ,Surface plasmon resonance ,Photocatalytic degradation ,Photodegradation ,Visible spectrum - Abstract
Using AgNO3 as the Ag source, the Ag@BiOCl photocatalyst was prepared by depositing noble metal Ag on the surface of BiOCl by solvothermal method. Ag exists as spherical particles with a diameter of 40 nm. Based on the surface plasmon resonance effect (SPR) of Ag, the photoresponse range of BiOCl was successfully extended to the visible light region, which reduced the photo-generated electron-hole recombination efficiency and improved the charge transfer efficiency. The photocatalytic performance of Ag@BiOCl was studied under visible light. The results show that when the Ag content is 10wt%, it exhibits excellent effects on the degradation of acid red B (ARB), and its degradation rate constant is 11 times larger than that of BiOCl. The active substance-capturing test results show that the main role in photocatalytic degradation of ARB is voids.
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- 2021
20. Analyses of the clinical and immunological characteristics of patients with lupus erythematosus
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Min, Deng, Ruifang, Wu, Xingyu, Zhou, Yuwen, Su, and Yaping, Li
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Lupus erythematosus (LE) is a broad-spectrum, heterogeneous disease. At one end of the spectrum is the cutaneous LE (CLE) without systemic involvement, and at the other end is the systemic LE (SLE) with multisystem involvement. Analyses of clinical and immunological indicators and pathological examinations are helpful for early diagnosis, differential diagnosis, and prognosis of LE.We described the clinical and laboratory characteristics of patients with LE and assessed the diagnostic value of immunohistochemical detection of C3d, C4d, IgG, IgG4, and CD123 in skin lesions of LE.Clinical and laboratory data of 62 patients with LE were collected. The expression levels of C3d, C4d, IgG, IgG4, and CD123 in skin lesions of LE were detected by immunohistochemistry (IHC).Clinical manifestations such as hematological involvement, C3, C4, ESR, hematuresis, proteinuria, anti-Sm, anti-ribosomal P-protein, anti-U1-RNP, anti-histone, and anti-nucleosome antibodies are helpful for classificatory diagnosis of LE. The positive rate of C3d and/or C4d along the basement membrane zone in LE skin lesions by IHC was 74.6%, which was higher than that by direct immunofluorescence (47.5%) (The diagnosis of CLE and SLE requires a combination of clinical manifestations, laboratory indicators, and pathological examination. Immunohistochemical detection of C3d, C4d, and CD123 in skin lesions is important for the classificatory diagnosis of LE.
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- 2022
21. It is not worth postponing frozen embryo transfers after oocyte pickup: A retrospective cohort study based on propensity score matching
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Mengxia, Ji, Bihui, Jin, Xiaoyan, Guo, Ruifang, Wu, Yunqing, Jiang, Ling, Zhang, and Jing, Shu
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Cryopreservation ,Pregnancy ,Endocrinology, Diabetes and Metabolism ,Birth Weight ,Humans ,Oocyte Retrieval ,Female ,Embryo Transfer ,Propensity Score ,Retrospective Studies - Abstract
This study was to explore whether postponing frozen embryo transfers (FET) after oocyte pickup (OPU) improves clinical and neonatal outcomes. From May 2018 to Dec 2020, a total of 1109 patients underwent their first OPU cycles adopting a non-selective freeze-all policy were included in this retrospective cohort study. In the immediate group (n=219), patients underwent FET in the first menstrual cycle after OPU, and patients in the postponed group (n=890) waited for more than 1 menstrual cycle after OPU to perform FET. A propensity score matching (PSM) model was used to evaluate the clinical outcomes and neonatal outcomes between the two groups. There were 209 patients in the immediate group and 499 patients in the postponed one after PSM. Patients waited for a significantly shorter period for FET in the immediate group (30.74 ± 3.85 days) compared with the postponed group (80.39 ± 26.25 days, P 0.05). No statistical significance was found in the average birth weight (3088.82 ± 565.35 g vs 3038.64 ± 625.78 g, P > 0.05) and height (49.08 ± 1.87 cm vs 49.30 ± 2.52 cm) of neonates between the two groups. The gender ratio, the incidence of macrosomia and low birth weight did not differ significantly between the two groups. In conclusion, postponing FET does not improve clinical and neonatal outcomes. If patients have no contraindications, FETs should be carried out immediately after OPU.
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- 2022
22. Enhancement of Cu
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Xin, Huang, Haitao, Li, Yin, Zhang, Ruifang, Wu, Lijun, Ban, Lin, Xi, Zhifang, Yin, Jian, Peng, Yongxiang, Zhao, and Li, Fang
- Abstract
In conventional thermocatalytic reactions under a reducing atmosphere, stabilization of the active Cu
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- 2022
23. Clinical features and prognostic factors of severe cutaneous adverse drug reactions: A single-center retrospective study of 209 cases in China
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Min Deng, Yuwen Su, Ruifang Wu, Siying Li, Guishao Tang, Qiqi Kuang, Xin Luo, Yanshan Zhu, and Weiyun Shen
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Pharmacology ,Immunology ,Immunology and Allergy - Abstract
Severe cutaneous adverse drug reactions (SCAR) are life-threatening and contain drug reactions with eosinophilia and systemic symptoms (DRESS), Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN), and acute generalized exanthematous pustulosis (AGEP).We aimed to evaluate clinical features and prognostic factors for SCAR patients. From January 2010 to April 2022, 209 patients with SCAR (DRESS, n = 46, SJS/TEN, n = 128, AGEP, n = 35) were included in this study. Clinical symptoms, laboratory tests, causative drugs, disease courses, treatments, and outcomes were investigated.Antibiotics ranked first (35.9 %) followed by traditional Chinese medicine (15.8 %) and antiepileptic drugs (14.8 %) among causative drugs of SCAR. One patient (2.2 %) with DRESS and seven patients (5.5 %) with SJS/TEN died in the hospital, while there was no AGEP-related mortality. The multivariate logistic regression analysis showed that high Registry of Severe Cutaneous Adverse Reactions score (OR = 2.340, 95 % CI = 1.192-4.591) and hemoglobin 100 g/L (OR = 0.126, 95 % CI = 0.016-0.983) were independent risk factors of DRESS. Anemia (OR = 0.191, 95 % CI = 0.037-0.984) and body surface area detached involved at day 1 (OR = 2.749, 95 % CI = 1.115-6.778) were independent risk factors of SJS/TEN for severe acute complications and hospital death (P 0.05). Lymphocytopenia (OR = 0.004, 95 % CI = 0.000-0.553) was a risk factor of AGEP for acute complications (P = 0.028).This study reveals the clinical features and independent prognostic factors for SCAR, which may be helpful in the clinical management for SCAR patients.
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- 2022
24. Single-cell transcriptome reveals immunopathological cell composition of skin lesions in subacute cutaneous lupus erythematosus
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Meiling Zheng, Zhi Hu, Wenhui Zhou, Yi Kong, Ruifang Wu, Bo Zhang, Hai Long, Sujie Jia, Qianjin Lu, and Ming Zhao
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Keratinocytes ,B-Lymphocytes ,Immunology ,Lupus Erythematosus, Cutaneous ,Immunology and Allergy ,Humans ,Transcriptome ,Skin - Abstract
Subacute cutaneous lupus erythematosus (SCLE) is a clinical subtype of cutaneous lupus erythematosus with psoriatic-like or annular papules with scaly erythemas, the pathological mechanism of which is poorly understood. To investigate the immune pathogenesis of SCLE, we performed single-cell RNA sequencing (scRNA-seq) of SCLE skin lesions and integrated the scRNA-seq data from skin tissues of healthy controls (HCs). Our results identified expanded fibroblasts and keratinocytes subtypes, abnormally activated lymphocyte and inflammatory M1 macrophages in SCLE. In SCLE, stromal cells, such as keratinocytes and fibroblasts, showed enhanced chemotactic functions for recruiting immune cells. Importantly, interferon-related genes were identified as key intermediate genes in the potential trajectory of fibroblasts, keratinocytes, and B cells from HCs to SCLE. Our investigation provides a comprehensive description of cell composition in SCLE and highlights several important clues for understanding the pathogenesis of SCLE.
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- 2022
25. Comparison of cycle threshold values of the Cobas HPV test and viral loads of the BMRT HPV test in cervical cancer screening
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Qing Yang, Hui Du, Xinfeng Qu, Wenkui Dai, Liming Gui, Changzhong Li, Chun Wang, Chunlei Guo, Yi Zhang, Lihui Wei, J. L. Belinson, and Ruifang Wu
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Clinical Trials as Topic ,Papillomavirus Infections ,Public Health, Environmental and Occupational Health ,Humans ,Uterine Cervical Neoplasms ,Female ,Middle Aged ,Viral Load ,Uterine Cervical Dysplasia ,Early Detection of Cancer - Abstract
ObjectiveTo validate the HPV viral loads that are reflected by the cycle threshold values of Cobas4800 as the viral load indicators by verifying the consistency of the viral loads per unit (10,000 cells) from the BMRT assay.MethodsThe analysis is based on data from the Chinese Multi-Center Screening Trial (CHIMUST). The cases included in the analysis are all positive for physician-collected hrHPV on SeqHPV and/or Cobas4800 or negative for hrHPV but abnormal in cytology (≥LSIL), and some cases selected by nested case-control randomization from those negative for physician-collected hrHPV and cytology. With HPV testing results and relevant Ct values from Cobas4800 available, we tested the entire sample set with the BMRT HPV testing assay and analyzed their agreement with Cobas4800, followed by a comparison of the CtV from Cobas4800 and viral loads (lg) from BMRT by lesion grade.ResultsWe included 4,485 women (mean age: 45.4 years) in the study, and 4,290 had complete data. The consistency of genotypes from Cobas4800 and BMRT for hrHPV, HPV-16, HPV-18, and 12-HPV pools was 94.9% (4070/4290, Kappa = 0.827), 99.1% (4251/4290, Kappa = 0.842), 99.6% (4,273/4,290, Kappa = 0.777), and 95.3% (4,089/4,290, Kappa = 0.821), respectively. Further analysis shows that any inconsistency between the two assays is likely among samples with comparatively lower viral loads. When analyzing per lesions of CIN2+ and CIN3+, the CtV from Cobas4800 and VL (lg) from BMRT are highly correlated inversely and follow the linear regression for HPV16 and 12-HPV pool (Pearson's or Spearman's correlation coefficient (r): In CIN3+, r HPV16 = −0.641, P < 0.001; r 12−HPVpool = −0.343, P = 0.109; In CIN2+, r HPV16 = −0.754, P < 0.001; r 12−HPVpool = −0.429, P < 0.001).ConclusionThe CtV from Cobas4800 and the viral loads (lg) of per unit cells from the BMRT are well correlated for lesion grading when tested on physician-collected samples. Cobas-CtV is worthy of further study for clinical application.
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- 2022
26. Acne conglobata caused by Cutibacterium avidum
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Jiao Jiang, Jun Tang, Yixin Tan, Ruifang Wu, and Shuaihantian Luo
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Immunology and Allergy ,Dermatology - Published
- 2022
27. Auranofin ameliorates psoriasis-like dermatitis in an imiquimod-induced mouse by inhibiting of inflammation and upregulating FA2H expression
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Xin Luo, Yuwen Su, Lian Zhong, Qiqi Kuang, Yanshan Zhu, Xiao Zhou, Guishao Tang, Yunfeng Fu, Siying Li, and Ruifang Wu
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Pharmacology ,General Medicine - Published
- 2023
28. Novel polymorphism of IFI44L associated with the susceptibility and clinical characteristics of systemic lupus erythematosus in a Chinese population
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Qing Zhang, Ruifang Wu, Yixin Tan, Junke Huang, and Shuaihantian Luo
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Pharmacology ,Immunology ,Immunology and Allergy - Published
- 2023
29. The dysregulation of circulating innate lymphoid cells is related to autoantibodies in pemphigus vulgaris
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Yanshan Zhu, Jian Su, Peng Zhang, Min Deng, Ruifang Wu, Yidan Liu, Yuwen Su, and Siying Li
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Pharmacology ,Immunology ,Immunology and Allergy - Published
- 2023
30. The temperature stability of dielectric properties in NBT-xBT@BT-BZN ceramics
- Author
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Linlin Liang, Aizhen Song, Bian Gang, Ruifang Wu, and Jing Wang
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010302 applied physics ,Materials science ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,visual_art ,Phase (matter) ,0103 physical sciences ,Composite ceramic ,visual_art.visual_art_medium ,Ceramic ,Composite material ,0210 nano-technology - Abstract
NBT-xBT@BT-BZN composite ceramic materials were prepared by wet solid-phase reaction method. The effects of NBT-xBT (x = 6, 7, 8, 9 mol%) on the phase structure, morphology and dielectric propertie...
- Published
- 2021
31. Comparison of two methods for evaluating lower urinary tract symptoms in cervical cancer patients following radical hysterectomy
- Author
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Ruifang Wu, Zhiqi Wang, Yu-Mei Wu, Qiubo Lv, Jinsong Han, Lingying Wu, Yanlong Wang, Aiming Lv, Hongxia Li, Xiuliu Sun, Hongwu Wen, Sumei Wang, Huan Li, Fang An, Yunong Gao, Sha Wang, Lu-Wen Wang, and Qing Liu
- Subjects
Cervical cancer ,medicine.medical_specialty ,business.industry ,Urinary system ,Diagnostic concordance ,Urinary incontinence ,medicine.disease ,Overactive bladder ,Lower urinary tract symptoms ,Internal medicine ,Medicine ,medicine.symptom ,Radical Hysterectomy ,Pelvic lymphadenectomy ,business - Abstract
Objective To compare the degree of agreement and consistency of urodynamic studies (UDS) with low urinary tract symptoms (LUTS) questionnaires for evaluating LUTS in cervical cancer patients following radical hysterectomy (RH) and pelvic lymphadenectomy. Methods From January 2012 to March 2015, 204 cervical cancer patients who underwent RH in 13 hospitals were evaluated using the Incontinence Questionnaire-Female Lower Urinary Tract Symptoms (ICIQ-FLUTS) and the Overactive Bladder Symptom Score (OABSS). Urodynamic tests were also performed on these patients during the same period. Results Study participants’ age ranged from 23 to 75 years, with a mean (standard deviation) of 48.0 ± 9.3 years. Using questionnaires, the prevalence of patients with LUTS symptoms, including storage symptoms, voiding symptoms, stress urinary incontinence (SUI) and overactive bladder (OAB) was 86.3%, 77.0%, 62.7%, 52.9% and 14.7%, respectively. For UDS, the corresponding prevalence was 89.7%, 70.1%, 66.7%, 46.6% and 13.2%, respectively. The diagnostic concordance of questionnaires and UDS for storage symptoms, voiding symptoms, SUI and OAB was 79.9%, 66.7%, 66.7%, 57.4% and 79.9%, respectively. For voiding symptoms, the correlation coefficient was 0.272, which was higher than that of storage symptoms, SUI and OAB. Conclusions In cervical cancer patients who have undergone RH, there was a moderate degree of agreement between UDS and symptom questionnaires in evaluating LUTS, but the consistency was poor. Medical personnel should be adequately trained in UDS to ensure LUTS are adequately diagnosed in patients.
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- 2021
32. Cervicovaginal microbiota significantly changed for HPV-positive women with high-grade squamous intraepithelial lesion
- Author
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Chunlei Guo, Wenkui Dai, Qian Zhou, Liming Gui, Han Cai, Di Wu, Jun Hou, Changzhong Li, Shuaicheng Li, Hui Du, and Ruifang Wu
- Subjects
Microbiology (medical) ,Vaginal Smears ,Microbiota ,Squamous Intraepithelial Lesions ,Immunology ,Papillomavirus Infections ,Uterine Cervical Neoplasms ,Uterine Cervical Dysplasia ,Microbiology ,Infectious Diseases ,Carcinoma, Squamous Cell ,Humans ,Female ,Papillomaviridae - Abstract
Lower female genital tract is colonized by a variety of microbes (cervicovaginal microbiota, CVM) which associate with the risk of genital infection. This study characterized CVM for 149 Chinese women with different status of human papillomavirus (HPV) infection and squamous intraepithelial lesion (SIL): no HPV infection (HPV-), HPV infection without significant SIL (HPV+NoSIL), HPV infection with low-grade SIL (HPV+LSIL) and HPV infection with high-grade SIL (HPV+HSIL). Analysis results showed CVM has dramatically changed in HPV+HSIL group when compared to HPV+LSIL group, but it exhibited no significant differences between HPV- and HPV+NoSIL groups as well as between HPV+NoSIL and HPV+LSIL groups. In consistence, random forest analysis found more notable differences in HPV+HSIL vs HPV+LSIL comparison than in other comparisons. In addition, depletion of Lactobacillus in CVM was more to be frequently identified in SIL-positive women as compared to SIL-negative individuals. Our findings suggested that significant CVM differences occurred when SIL developed to HSIL which was caused by persistent HPV infection.
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- 2022
33. The association of cervicovaginal Langerhans cells with clearance of human papillomavirus
- Author
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Wenkui Dai, Liming Gui, Hui Du, Shuaicheng Li, and Ruifang Wu
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Langerhans Cells ,Immunology ,Papillomavirus Infections ,Immunology and Allergy ,Humans ,Female ,Cervix Uteri ,Alphapapillomavirus ,Papillomaviridae - Abstract
Human papillomavirus (HPV) clearance is important in eliminating cervical cancer which contributes to high morbidity and mortality in women. Nevertheless, it remains largely unknown about key players in clearing pre-existing HPV infections. HPV antigens can be detected by the most important cervical antigen-presenting cells (Langerhans cells, LCs), of which the activities can be affected by cervicovaginal microbiota. In this review, we first introduce persistent HPV infections and then describe HPV-suppressed LCs activities, including but not limited to antigen uptake and presentation. Given specific transcriptional profiling of LCs in cervical epithelium, we also discuss the impact of cervicovaginal microbiota on LCs activation as well as the promise of exploring key microbial players in activating LCs and HPV-specific cellular immunity.
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- 2022
34. Characteristics of genotypes and prevalence of high-risk human papillomavirus in different economic development regions in China
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Jingran Li, Chao Zhao, Ruifang Wu, Mingzhu Li, Yun Zhao, Hui Du, Ling Li, Zhixin Lin, Zhijun Zhang, and Lihui Wei
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Microbiology (medical) ,China ,Infectious Diseases ,Genotype ,DNA, Viral ,Papillomavirus Infections ,Prevalence ,Humans ,Uterine Cervical Neoplasms ,Female ,Economic Development ,Alphapapillomavirus ,Papillomaviridae - Published
- 2022
35. Influence of Nb2O5 doping to energy-storage properties of (Na0.5Bi0.5)0.705Ba0.045Sr0.25TiO3 lead-free ferroelectric ceramics
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Jinghao Zhao, Jing Wang, Linlin Liang, Zekai Li, Gang Bian, Ruifang Wu, and Ruijie Duan
- Subjects
010302 applied physics ,Materials science ,Ferroelectric ceramics ,Doping ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Ferroelectricity ,Energy storage ,Electronic, Optical and Magnetic Materials ,0103 physical sciences ,Composite material ,0210 nano-technology - Abstract
The powder samples of (Na0.5Bi0.5)0.705Ba0.045Sr0.25TiO3-xNb2O5 (NBBSNT) were synthesized by a wet solid-phase method. The effects of Nb-doping on morphology, ferroelectric and dielectric propertie...
- Published
- 2021
36. Differential diagnosis of high-grade squamous intraepithelial lesions and benign atrophy in older women using p16 immunocytochemistry
- Author
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Ruifang Wu, Hui Du, Fangbin Song, Chun Wang, Xia Huang, Yingjie Luo, and Peisha Yan
- Subjects
Cervical cancer ,Colposcopy ,medicine.medical_specialty ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Population ,Immunocytochemistry ,medicine.disease ,Gastroenterology ,Atrophy ,Internal medicine ,Cytology ,medicine ,Biomarker (medicine) ,Differential diagnosis ,education ,business - Abstract
Background For cervical cancer screening, routine cytology has a high specificity but a lower sensitivity. In older women, atrophy, which may mimic HSIL, presents a diagnostic challenge. p16 is a widely used biomarker for histological diagnosis of HSIL. Our objective was to evaluate PathCIN® p16INK4a immunocytology in identification of high grade dysplasia vs. benign atrophy. Methods As part of a multi-center screening program, 3351 women were co-tested by p16 immunocytology. Among women referred for colposcopy on basis of cytology and high-risk HPV status, those with atrophy were older than the population screened (52 vs. 43 years). Cases from older women with atrophy (n = 116) and controls without atrophy (n = 47) were identified by re-examination of Pap smears. The detection of CIN2+ was compared for p16, cytology and HR-HPV results. Results The sensitivity of routine cytology (≥LSIL) was much lower for cases with atrophy (17%) than non-atrophic cases (75%). The sensitivity of p16 immunocytology and of HR-HPV testing was high (88%–100%) both with and without atrophy. The specificity of routine cytology (≥LSIL) was higher for cases with atrophy (79%) than non-atrophic cases (38%). The specificity of p16 immunocytology was high (88–95%) and the specificity of HR-HPV testing was low (31%–33%) both with and without atrophy. Combining p16 with HPV testing and/or routine cytology had no benefit, as compared to p16 staining alone. Conclusions p16 immunocytology compares favorably with routine cytology and HPV testing in the differential diagnosis of HSIL and benign atrophy. It is more sensitive than cytology for atrophic specimens, and is more specific than HPV testing. p16 immunocytology may decrease the need for colposcopy referrals and could be a useful tool for early detection of cervical cancer in peri- and post-menopausal women, who are more likely to have HSIL coexisting with atrophy.
- Published
- 2021
37. Investigation of Different Apatites-Supported Co3O4 as Catalysts for N2O Decomposition
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Xiaoli Liu, Ruifang Wu, Yongzhao Wang, and Yongxiang Zhao
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Chemistry ,Fluorapatite ,02 engineering and technology ,General Chemistry ,Atmospheric temperature range ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Decomposition ,Catalysis ,Apatite ,0104 chemical sciences ,Chemical engineering ,Impurity ,visual_art ,Desorption ,Specific surface area ,visual_art.visual_art_medium ,0210 nano-technology - Abstract
Two types of apatite supports including hydroxyapatite (HAP) and fluorapatite (FAP) were prepared by the co-precipitation method. Supported Co catalysts with the above carriers were synthesized from Co(NO3)2·6H2O via the impregnation method. The catalytic activity for N2O decomposition was investigated using a continuous flowing microreactor system. The catalytic results demonstrated that the Co/HAP exhibited more excellent catalytic activity as well as the impurity gas (H2O, O2, and NO) resistance capability than the Co/FAP in the temperature range of 300–600 °C. Furthermore, the physicochemical properties of Co/HAP and Co/FAP were determined by several relevant characterization methods. The obtained experimental results revealed that Co/HAP possessed larger specific surface area, more ion-exchanged Co2+ and stronger oxygen desorption capacity than Co/FAP, which are the key factors for high N2O decomposition activity. Moreover, compared with Co/FAP, Co/HAP has not only more basic content, but also higher basic site density, which may be also conducive to enhance the electron donating ability of Co2+, thereby promoting the N2O catalytic decomposition.
- Published
- 2021
38. Evaluation of Cobas HPV and SeqHPV Assays in the Chinese Multicenter Screening Trial
- Author
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Hua Jin, Hui Du, Chun Wang, Wei Zhang, Hanyi Li, Yan Liu, Hongxue Luo, Jingran Li, Xia Huang, Juan Liu, Jerome L. Belinson, Li-hui Wei, Xianzhi Duan, Bin Shi, Aimin Xiao, Xinfen Qu, Juncui Bao, Shuhuang Bian, L P Duan, Jing Jiang, Chao Zhao, Ruifang Wu, and Lijie Zhang
- Subjects
Adult ,Human Papillomavirus Positive ,China ,medicine.medical_specialty ,Complete data ,Genotype ,Large population ,Uterine Cervical Neoplasms ,Cervix Uteri ,Cervical cancer screening ,Cervical intraepithelial neoplasia ,Sensitivity and Specificity ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Papillomaviridae ,Early Detection of Cancer ,Vaginal Smears ,Gynecology ,Colposcopy ,030219 obstetrics & reproductive medicine ,medicine.diagnostic_test ,business.industry ,Screening Trial ,Papillomavirus Infections ,HPV infection ,Obstetrics and Gynecology ,General Medicine ,Middle Aged ,Uterine Cervical Dysplasia ,medicine.disease ,Self Care ,030220 oncology & carcinogenesis ,Female ,business - Abstract
Objective The aim of the study was to evaluate the Cobas 4800 Assay and the SeqHPV Assay with self (S) and direct (D) cervical samples in the Chinese Multicenter Screening Trial (CHIMUST). Materials and methods The CHIMUST is a large population-based multicenter clinical trial, and 10,885 women aged 30-59 years from 15 sites in 7 provinces with no cervical cancer screening for 3 years were eligible. All participating women contributed one self-collected sample (S) and 1 physician-collected endocervical sample (DL). The self-collected sample was first applied to the solid media transport card (SS), and then, the brush placed in 6 mL of ThinPrepSolution (SL). All samples were tested with Cobas 4800 and SeqHPV high-risk HPV assays. Patients human papillomavirus positive (self or direct) were recalled for colposcopy and biopsies. Results A total of 10,399 women had complete data. The mean age was 43.9 years. A total of 1.4% (142/10,399) had cervical intraepithelial neoplasia (CIN) 2+ and 0.5% (54/10,339) had CIN 3+. In the liquid specimens, the overall HPV infection rates were 10.8% for Cobas and 10.9% for SeqHPV in D sample, and 13.7% for Cobas and 11.6% for SeqHPV in SL sample, respectively. The sensitivity of Cobas-DL, Cobas-SL, SeqHPV-DL, and SeqHPV-SL for CIN 2+ was 95.07%, 95.07%, 94.33%, and 96.48%, respectively. The specificity of Cobas-DL, Cobas-SL, SeqHPV-DL, and SeqHPV-SL for CIN 2+ was 90.38%, 87.35%, 90.21%, and 89.53%, respectively. There were no differences in sensitivity when applying the 2 assays to both self- and directly collected samples in liquid transport media (p > .05). Conclusions Both Cobas and SeqHPV screening assays using both self-collected and directly endocervical collected specimens demonstrate similar sensitivity for the detection of CIN 2+ and CIN 3+.
- Published
- 2021
39. A facile complexing chemical reduction for the preparation of Sn/graphene nanocomposites and their high performance for lithium-ion batteries
- Author
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Yong Li, Haiting Ren, Yun Zhao, Zhouru Guo, Canliang Ma, Ruifang Wu, Cheng-meng Chen, and Yongxiang Zhao
- Subjects
Mechanics of Materials ,Mechanical Engineering ,Materials Chemistry ,Metals and Alloys - Published
- 2023
40. Panoramic view of clinical features of lupus erythematosus: a cross-sectional multicentre study from China
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Hui Jin, Shihang Zhou, Yangyiyi Yu, Ming Zhao, Haijing Wu, Hai Long, Siqi Fu, Ruifang Wu, Heng Yin, Jieyue Liao, Shuangyan Luo, Yu Liu, Qing Zhang, Peng Zhang, Yixin Tan, Shuaihantian Luo, Xin Huang, Fen Li, Guanghui Ling, and Qianjin Lu
- Subjects
Rheumatology ,General Medicine - Abstract
ObjectiveLupus erythematosus (LE) is a complicated disease with highly heterogeneous clinical manifestations. Previous studies have rarely included all subgroups of patients with lupus and have overlooked the importance of the cutaneous manifestations thereof. We aimed to compare the demographic and clinical differences among patients with different subtypes of lupus.MethodsThis is the first real-world study with a relatively large sample size that simultaneously includes patients with isolated cutaneous lupus erythematosus (iCLE) and SLE. All samples were obtained from the Lupus Erythematosus Multicenter Case–control Study in Chinese populations (LEMCSC) (registration number: ChiCTR2100048939). Comparative analyses between different LE subgroups were performed.ResultsA total of 2097 patients with lupus were included, with 1865 patients with SLE, 1648 with cutaneous lupus erythematosus (CLE), and 232 with iCLE. Among the patients with CLE, 1330 had acute cutaneous lupus erythematosus (ACLE); 160 had subacute cutaneous lupus erythematosus (SCLE); and 546 had chronic cutaneous lupus erythematosus (CCLE). The study included a relatively large number of patients with CCLE subtypes, including 311 with discoid lupus erythematosus (DLE), 262 with chilblain lupus erythematosus (CHLE) and 45 with lupus erythematosus profundus (LEP). Demographic characteristics, systemic involvement, mucocutaneous manifestations and autoantibodies were significantly different among the groups.ConclusionsCLE and iCLE are two distinct disease states, and the selection of broad or narrow CLE definitions should be emphasised in scientific reports. LE-non-specific cutaneous lesions imply more severity, while self-reported photosensitivity and LE-specific cutaneous manifestations imply milder severity. Generalised ACLE appears to be a more severe state than localised ACLE, and CHLE appears to be more severe than DLE. Anti-Sjögren’s syndrome-related antigen B (SSB) antibodies have higher specific directivity than anti-Sjögren’s syndrome-related antigen A (SSA) antibodies for SCLE lesions. Anti-double-stranded DNA antibodies have a higher co-occurrence with ACLE and a lower co-occurrence with SCLE and CCLE. Compared with DLE, CHLE has significantly higher positive rates of anti-SSA/Ro60 (71%) and anti-SSA/Ro52 (42.4%) antibodies, whereas LEP is associated with a higher positive rate of antinucleosome antibodies (31.1%).
- Published
- 2023
41. Successful Ultrasound-Guided Methotrexate Intervention in the Treatment of Heterotopic Interstitial Pregnancy: A Case Report and Literature Review
- Author
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Ping Li, Xiao Tan, Yi Chen, Qiaoli Ge, Haiying Zhou, Renrong Zhang, Yue Wang, Min Xue, Ruifang Wu, and Desheng Sun
- Subjects
Medicine (miscellaneous) - Abstract
Purpose: This study aims to share the experience of minimally invasive ultrasound-guided methotrexate intervention in the treatment of heterotopic interstitial pregnancy (HIP) with good pregnancy outcomes, and to review the treatment, pregnancy outcomes, and impact on the future fertility of HIP patients. Methods: The paper describes the medical history, clinical manifestations, treatment history, and clinical prognosis of a 31-year-old woman with HIP, and reviews cases of HIP from 1992 to 2021 published in the PubMed database. Results: The patient was diagnosed with HIP by transvaginal ultrasound (TVUS) at 8 weeks after assisted reproductive technology. The interstitial gestational sac was inactivated by ultrasound-guided methotrexate injection. The intrauterine pregnancy was successfully delivered at 38 weeks of gestation. Twenty-five HIP cases in 24 studies published on PubMed from 1992 to 2021 were reviewed. Combined with our case, there were 26 cases in total. According to these studies, 84.6% (22/26) of these cases were conceived by in vitro fertilization embryo transfer, 57.7% (15/26) had tubal disorders, and 23.1% (6/26) had a history of ectopic pregnancy; 53.8% (14/26) of the patients presented with abdominal pain and 19.2% (5/26) had vaginal bleeding. All cases were confirmed by TVUS. In total, 76.9% (20/26) of intrauterine pregnancies had a good prognosis (surgery vs. ultrasound interventional therapy 1:1). All fetuses were born without abnormalities. Conclusions: The diagnosis and treatment of HIP remain challenging. Diagnosis mainly relies on TVUS. Interventional ultrasound therapy and surgery are equally safe and effective. Early treatment of concomitant heterotopic pregnancy is associated with high survival of the intrauterine pregnancy.
- Published
- 2023
42. Thermocoagulation versus cryotherapy for the treatment of cervical precancers
- Author
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Chun Wang, Ruifang Wu, Jerome L. Belinson, Aimin Xiao, Liwei Zhao, Lyufang Duan, Xinfeng Qu, Zhihong Liu, Hui Du, and Shuangyan Liu
- Subjects
Vaginal discharge ,medicine.medical_specialty ,medicine.medical_treatment ,efficacy ,Uterine Cervical Neoplasms ,complication ,Cryotherapy ,Atypical Squamous Cells ,cervical intraepithelial neoplasia ,Cervical intraepithelial neoplasia ,thermocoagulation ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Cytology ,Biopsy ,Electrocoagulation ,medicine ,Humans ,Papillomaviridae ,Vaginal Smears ,Colposcopy ,030219 obstetrics & reproductive medicine ,treatment ,medicine.diagnostic_test ,business.industry ,Papillomavirus Infections ,Obstetrics and Gynecology ,Original Articles ,Uterine Cervical Dysplasia ,medicine.disease ,Surgery ,030220 oncology & carcinogenesis ,Original Article ,Female ,medicine.symptom ,business ,Complication - Abstract
Aim To compare thermocoagulation and cryotherapy for treatment of high‐grade cervical intraepithelial neoplasia (CIN). Methods From May 2017 to May 2018, women with CIN2/3 were randomized to thermocoagulation or cryotherapy at Peking University Shenzhen Hospital. Follow‐up at 4 and 8 months included cytology and human papillomavirus (HPV) testing. Women who were HPV‐positive or had atypical squamous cells of undetermined significance or higher‐grade disease underwent colposcopy/biopsy. Results Among 149 women enrolled, 74 were randomized to thermocoagulation, and 75 to cryotherapy (excluded four were immediately referred for thermocoagulation due to large lesions). At follow‐up, there was no difference between the thermocoagulation and cryotherapy groups in HPV‐negative (4/8 months: 72.5%/86.2% vs 68.6%/80.6%) and pathology‐negative (97.1%/98.5% vs 94.3%/92.3%) rates (all P > 0.05). The cytology‐negative rate was similar for thermocoagulation and cryotherapy at 4 months (79.7% vs 78.9%, P > 0.05), but higher for thermocoagulation at 8 months (100% vs 88.7%, P
- Published
- 2020
43. Prevalence of autoimmune thyroid disease in patients with psoriasis: a meta-analysis
- Author
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Xiaochao Zhang, Suhan Zhang, Ruifang Wu, Siying Li, Yuwen Su, and Peng Zhang
- Subjects
immunology ,Observational Studies as Topic ,Prevalence ,Medicine ,Humans ,Psoriasis ,thyroid disease ,General Medicine ,Hashimoto Disease ,Dermatology - Abstract
ObjectivePsoriasis is a chronic inflammatory disease with autoimmune aetiology. A possible link between psoriasis and autoimmune thyroid disease (AITD) has been suggested in some studies with inconsistent findings. This meta-analysis aims to determine the association between psoriasis and AITD.DesignA meta-analysis of observational studies.Data sourcesPubMed, EMBASE, Scopus and the Cochrane Library were searched up to 1 November 2021.Eligibility criteria for selecting studiesWe included non-randomised studies, each with over 50 cases in every group, focusing on the rate of comorbidity between psoriasis and AITD.Data extraction and synthesisTwo independent reviewers screened the articles and extracted data. The restricted maximum-likelihood was applied to perform the meta-analysis. OR and 95% CIs were pooled to compare the prevalence of AITD in psoriasis and control groups. Heterogeneity was assessed with I2 statistic. The Newcastle-Ottawa Scale and Agency for Healthcare Research and Quality were applied for quality assessment. The risk of bias was assessed with Risk Of Bias In Non-randomised Studies-of Interventions (ROBINS-I).ResultsEleven available studies with data on 253 313 patients with psoriasis and 1 376 533 controls were included. Meta-analysis showed that patients with psoriasis had a higher prevalence of AITD (OR 1.76, 95% CI 1.35 to 2.28, Z=4.25, pConclusionsOur study indicates that the rate of co-occurring AITD was significantly increased in patients with psoriasis. It suggests that the increased risk of AITD should be concerned in patients with psoriasis.PROSPERO registration numberCRD42020206005.
- Published
- 2022
44. Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus
- Author
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Shuaihantian Luo, Ruifang Wu, Qianwen Li, and Guiying Zhang
- Subjects
Article Subject ,Immunology ,Immunology and Allergy ,Cytokines ,Humans ,Lupus Erythematosus, Systemic ,General Medicine ,Dendritic Cells ,Interferons ,DNA Methylation ,Monocytes ,Epigenesis, Genetic - Abstract
Background. Interferon-inducible 44 like (IFI44L) is a newly discovered interferon-induced gene and has been reported to overexpress in systemic lupus erythematosus (SLE). However, little is known about the mechanism and function of IFI44L overexpression in SLE. In this study, we aimed to investigate the epigenetic mechanism of IFI44L overexpression in SLE monocyte and its potential functions contributing to the pathogenesis of SLE. Methods. We collected peripheral blood from 20 SLE patients and 20 healthy controls. Expression of IFI44L in monocytes and effects of different signal transducers and activators of transcription (STAT) pathway inhibitors on IFI44L expression were detected. Recruitment of ten-eleven translocation protein (TET) by STAT and methylation of IFI44L promoter were evaluated. Effects of IFI44L overexpression on the expression of surface markers on monocyte-derived dendritic cells (Mo-DCs) were analyzed. T cell differentiation mediated by Mo-DCs and related cytokines production were also analyzed. Results. Expression level of IFI44L was significantly increased in SLE monocyte. IFI44L expression was decreased most significantly in STAT3 inhibitor compared with other inhibitors. STAT3 regulated IFI44L expression and interacted with TET2 which induced DNA demethylation of IFI44L promoter. Overexpression of IFI44L in monocyte enhanced the maturation and functions of Mo-DC by upregulating costimulatory receptors and inducing Th1/Th17-related cytokines when cocultured with naïve CD4+ T cells. Conclusion. TET2 recruited by STAT3 induces DNA demethylation of IFI44L promoter which promotes IFI44L overexpression in monocyte contributing to the pathogenesis of SLE by enhancing the maturation and functions of Mo-DC. IFI44L is expected to become a new target for treatment of SLE.
- Published
- 2022
45. Verification of the association of the cycle threshold (Ct) values from HPV testing on Cobas4800 with the histologic grades of cervical lesions using data from two population-based cervical cancer screening trials
- Author
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Yi Zhang, Hui Du, Aimin Xiao, Wei Zhang, Chun Wang, Xia Huang, Xinfeng Qu, Jianliu Wang, and Ruifang Wu
- Subjects
Cancer Research ,Infectious Diseases ,Oncology ,Epidemiology - Abstract
Objective To verify the association of high-risk human papillomavirus (hrHPV) viral load reflected by cycle threshold (Ct) values from HPV testing on Cobas4800 assay with the histologic grades of cervical lesions via analysis on the combined data from two cervical cancer screening trials and to explore the referability of Ct values in management of the abnormalities from cervical cancer primary screening. Methods We analyzed the data from Chinese Multi-Center Screening Trial (CHMUST) and BUJI Cervical Cancer Screening Study Project (BUJI Study). All data to be analyzed in this paper were related to provider-collected samples. One-way ANOVA was performed to compare the Ct values among different histological groups, and Kendall’s tau-b correlation was applied to examine the association between Ct values and cervical lesion grades. The stepwise incidence of CIN2+ and CIN3+ in every 100 HPV positive individuals were calculated according to the descending of the genotype specific Ct values. The highest Ct values related to CIN3+ incidence rate 4% (or 25%) were used as the cutoffs to distinguish low-Ct value cases from the high-Ct value ones. Results A total of 1376 women in CHUMUST and BUJI Study who were aged 30–59 and positive of hrHPV for provider-collected samples on Cobas4800 with complete data in terms of the relevant Ct values (CtV) and the histological diagnosis were included for analysis. Our data showed significant difference among different histological grades of cervical lesions in the CtV of hrHPV, HPV16-plus (positive of HPV16 only or HPV16 plus 18 and/or pooled 12-HPV), and pooled 12-HPV (P P > 0.05). The CIN2+ or CIN3+ incidence per 100 positives significantly increased corresponding to the descending of the CtV of hrHPV, HPV16-plus and pooled 12-HPV. Compared with high-CtV groups (CtV > 33.2 for hrHPV, CtV > 29.6 for pooled 12-HPV), the relevant risks (RRs) of CIN2+ for hrHPV and pooled 12-HPV positive groups with low-CtV (CtV ≤ 33.2 and ≤ 29.6, respectively) were 3.2 (95%CI 2.18–4.80) and 2.3 (95%CI 1.50–3.45). Similarly, the RRs of CIN3+ for hrHPV and pooled 12-HPV positive groups with low-CtV were 6.5 (95%CI 2.83–14.80) and 2.7 (95%CI 1.15–6.39), respectively. The RRs of CIN2+ for medium- (30.3 37.4) groups, and the CIN3+ incidence in low-CtV value group was nine-fold higher of that in medium-CtV ones [RRs, 9.0 (95%CI 2.89–28.10)]. In comparing with the algorithms of “HPV16-plus/18-plus + cytology ≥ ASCUS for pooled 12-HPV”, triage algorithm “HPV16-plus/18-plus + Ct value ≤ 33.2 for pooled 12-HPV” could achieve a comparable sensitivity of 93.2%. Conclusion HPV viral loads reflected by Ct values for hrHPV, HPV16-plus and pooled 12-HPV from Cobas4800 HPV testing were directly associated with the severity of cervical lesions. A lower HPV genotype-specific Ct value prompted a significantly high CIN3+ risk of 4% or higher in women positive of hrHPV, HPV16-plus or pooled 12-HPV, indicating that HPV viral load reflected by Ct values on Cobas4800 may be a promising risk indicator in management of abnormalities from primary cervical cancer screening.
- Published
- 2022
46. A Panoramic View of Clinical Features of Lupus Erythematosus, a Cross-Sectional Multicenter Study from China
- Author
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Hui Jin, Shihang Zhou, Yangyiyi Yu, Tao Huang, Ming Zhao, Haijing Wu, Hai Long, Siqi Fu, Ruifang Wu, Heng Yin, Jieyue Liao, Shuangyan Luo, Yu Liu, Qing Zhang, Peng Zhang, Yixin Tan, Shuaihantian Luo, Xin Huang, Yaxiong Deng, Wei Liao, Liu Duan, Jianbo Chen, Yin Zhou, Jinghua Yin, Hong Qiu, Jin Yuan, Zijun Wang, Mengying Li, Xiaoqi Wu, Lina Chen, Liangmin Cai, Cancan Huang, Qianwen Li, Bingsi Tang, Bihui Yu, Xin Li, Xiaofei Gao, Yixi Hu, Xiaolei Ren, Haofan Xue, Zhangming Wei, Jinwei Chen, Fen Li, Guanghui Ling, Jia Wang, Hui Luo, Hongjun Zhao, Sen Yang, Yong Cui, Youkun Lin, Xu Yao, Lingyun Sun, Qing Guo, Hong Fang, Kang Zeng, Danqi Deng, Jianzhong Zhang, Yuzhen Li, Xiongming Pu, Xiangping Liao, Xiqiang Dang, Danlin Huang, Yumei Liang, Qing Sun, Hongju Xie, Li Zeng, Cibo Huang, Qingchun Diao, Juan Tao, Jianbin Yu, Zhenlu Li, Hanshi Xu, Hui Li, Wei Lai, Xiguang Liu, Jingjing Wu, Tienan Li, Tiechi Lei, Qiuning Sun, Yanjia Li, Guoqiang Zhang, and Qianjin Lu
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
47. Evaluating the Performance of p16INK4a Immunocytochemistry in Cervical Cancer Screening
- Author
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Peisha Yan, Chun Wang, Zhihong Liu, Xia Huang, Aimin Xiao, Xinfeng Qu, Hui Du, Fangbin Song, Ruifang Wu, and Jerome L. Belinson
- Subjects
0301 basic medicine ,Colposcopy ,Cervical cancer ,Oncology ,medicine.medical_specialty ,Cervical screening ,medicine.diagnostic_test ,business.industry ,Immunocytochemistry ,medicine.disease ,Cervical intraepithelial neoplasia ,Triage ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,Cytology ,Biopsy ,medicine ,business ,neoplasms - Abstract
Purpose When used for cervical cancer primary screening, liquid-based cytology (LBC) has a high specificity but a low sensitivity. For histological diagnosis of high-grade lesions, p16INK4a immunostaining has proven to be useful. Therefore, our objective was to evaluate the use of p16INK4a immuno-cytology as a primary screen and a secondary screen after primary high-risk human papillomavirus (hrHPV) screening or LBC screening. Methods A total of 1197 cytology slides were immuno-stained using automatic p16INK4a staining system (PathCIN®p16INK4a) in two studies from cervical screening programs. In the primary screening study, 875 slides were randomly selected and analyzed for p16INK4a. In the secondary screening study, 322 of the remaining slides were chosen by virtue of being HPV 16/18+, other hrHPV+/LBC≥ASC-US, or HPV-negative/LBC ≥LSIL. The sensitivity and specificity for detection of cervical intraepithelial neoplasia 2/3 or worse (CIN2+/CIN3+) were compared based on p16INK4a, LBC and HPV test results. Results In combining two studies, there were 431 cases with biopsy pathology. They included 83 cases with CIN2+ and 41 cases with CIN3+. The p16 positivity rate increased with pathologic and cytologic severity (P
- Published
- 2020
48. Effect of Ni 2+ chelated to the surface of <scp>PBFA</scp> on the charring flame retardant and smoke suppression properties of epoxy resin
- Author
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Hongjuan Wu, Tian Wang, Kailun Zhang, Ruifang Wu, Guang Yang, Jixing Xie, and Hongqiang Qu
- Subjects
Smoke ,Materials science ,Polymers and Plastics ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,Nickel ions ,Chelation ,General Chemistry ,Charring ,Epoxy ,Composite material ,Fire retardant - Published
- 2020
49. Preparation of BiOCl1-xIx solid solution and its visible light photocatalytic performance
- Author
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Zekai Li, Linlin Liang, Yun-Kai Lv, Ruifang Wu, Xiaoran Yan, and Jing Wang
- Subjects
010302 applied physics ,Materials science ,Quenching (fluorescence) ,Band gap ,Condensed Matter Physics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Specific surface area ,0103 physical sciences ,Photocatalysis ,Degradation (geology) ,Electrical and Electronic Engineering ,Science, technology and society ,Nuclear chemistry ,Solid solution ,Visible spectrum - Abstract
In this paper, BiOCl1-xIx (x = 0, 0.2, 0.4, 0.6, 0.8, and 1.0) solid solution was synthesized by one-step ultrasound-assisted solvothermal method. A series of samples with different band gap values was obtained by varying the amount of components. The synthesized photocatalysts were characterized by XRD, SEM, UV–Vis DRS, ESR, and BET specific surface area. The PL and EIS methods were used to evaluate the separation efficiency of the photogenerated electrons and holes. Photocatalytic experiments under visible light show that the degradation efficiency of BiOCl0.6I0.4 for acid red B (ARB) is 96.4% under 2 h irradiation time. After five times of recycling, the degradation rate of ARB was 91%. The results of free radical quenching experiments showed that the dominant role of BiOCl0.6I0.4 in the photocatalytic degradation of ARB is the oxidation of h+. A preliminary discussion on the photocatalytic mechanism was made.
- Published
- 2020
50. Type-specific Distribution of Cervical hrHPV Infection and the Association with Cytological and Histological Results in a Large Population-based Cervical Cancer Screening Program: Baseline and 3-year Longitudinal Data
- Author
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Zhihong Liu, Xia Huang, Chun Wang, Ruifang Wu, Hui Du, Fangbin Song, Aimin Xiao, Hongjian Men, and Meifang Zhao
- Subjects
Human papillomavirus ,medicine.medical_specialty ,genotype ,Population ,cervical cancer screening ,Large population ,Cervical intraepithelial neoplasia ,Cervical cancer screening ,03 medical and health sciences ,0302 clinical medicine ,Cytology ,Genotype ,medicine ,030212 general & internal medicine ,education ,Colposcopy ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Obstetrics ,vaccination ,medicine.disease ,female genital diseases and pregnancy complications ,Vaccination ,Oncology ,030220 oncology & carcinogenesis ,cytology ,business ,Research Paper - Abstract
Objectives: This study aimed to describe the study design, and to analyze the type-specific distribution of cervical high-risk human papillomavirus (hrHPV) infection and its association with cytological and histological results in a large population-based screening program in Buji Street, Shenzhen, China. Methods: A total of 10,186 women aged 21-70 years were co-tested by Cobas4800 HPV assay and liquid-based cytology. Women were referred to colposcopy by virtue of being HPV16/18-positive, Other hrHPV-positive/ cytology ≥ASCUS, or HPV-negative/ cytology ≥LSIL. Three-year histological follow-up data were recorded. Results: The overall prevalence of hrHPV infection was 11.1%; among them, the highest type was Other hrHPV (8.9%), followed by HPV16 (1.6%) and HPV18 (0.6%). Moreover, the prevalence of hrHPV and that of HPV16 increased with cytological severity (Ptrend
- Published
- 2020
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