4 results on '"Ruan, Jingjun"'
Search Results
2. Genome-Wide Identification and Characterization of The MADS-Box Gene Family and Its Expression in The Various Developmental Stage and Stress Conditions in Foxtail Millet (Setaria Italica)
- Author
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Dabing Xiang, Ruan Jingjun, Jun Yan, Liang Feng, Hao Yang, Li Long, Jianping Cheng, Yu Fan, Guoxing Xue, Xiaobao Wei, Ailing He, and Dili Lai
- Subjects
Genetics ,Setaria ,Developmental stage ,animal structures ,biology ,Foxtail ,Gene family ,Identification (biology) ,Stress conditions ,biology.organism_classification ,Genome ,MADS-box - Abstract
Foxtail millet (Setaria italica) is rich in nutrients and extremely beneficial to human health. We identified and comprehensively analyzed 89 MADS-box genes in the foxtail millet genome. According to the classification of MADS-box genes in Arabidopsis thaliana and rice, the SiMADS-box genes were divided into M-type (37) and MIKC-type (52). During evolution, the differentiation of MIKC-type MADS-box genes occurred before that of monocotyledons and dicotyledons. The SiMADS-box gene structure has undergone much differentiation, and the number of exons in the MIKC-type subfamily is much greater than that in the M-type subfamily. Analysis of gene duplication events revealed that MIKC-type MADS-box gene fragment duplication accounted for the vast majority of gene duplication events, and MIKC-type MADS-box genes played a major role in the amplification of SiMADS-box genes. Collinearity analysis showed highest collinearity between foxtail millet and maize MADS-box genes. Analysis of tissue-specific expression showed that SiMADS-box genes are highly expressed throughout the grain-filling process. Expression analysis of SiMADS-box genes under eight different abiotic stresses revealed many stress-tolerant genes, with induced expression of SiMADS33 and SiMADS78 under various stresses warranting further attention. Further, some SiMADS-box proteins may interact to cope with external stress. This study provides insights for MADS-box gene mining and molecular breeding of foxtail millet in the future.
- Published
- 2021
3. Effects of hydraulic loading rate and substrate on ammonium removal in tidal flow constructed wetlands treating black and odorous water bodies
- Author
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Yongqiang Yang, Runliang Zhu, Ruan Jingjun, Lingling Zhang, Fanrong Chen, Quan Zhang, and Shijun Wu
- Subjects
0106 biological sciences ,Environmental Engineering ,Nitrogen ,Bioengineering ,Wetland ,010501 environmental sciences ,01 natural sciences ,Waste Disposal, Fluid ,chemistry.chemical_compound ,010608 biotechnology ,Ammonium Compounds ,Substrate (aquarium) ,Ammonium ,Zeolite ,Waste Management and Disposal ,0105 earth and related environmental sciences ,geography ,geography.geographical_feature_category ,biology ,Renewable Energy, Sustainability and the Environment ,Water ,General Medicine ,Comammox ,Pulp and paper industry ,biology.organism_classification ,Microbial population biology ,chemistry ,Wetlands ,Denitrification ,Environmental science ,Nitrification ,Nitrospira - Abstract
The efficient removal of ammonium nitrogen (NH4+-N) is vital to eliminating black and odorous water bodies. In this work, tidal flow constructed wetlands with gravel (TFCW-G) and with a mixture of zeolite and gravel (TFCW-Z) were set up to treat black and odorous water bodies at different hydraulic loading rates (HLRs). Results showed that zeolite significantly enhanced nitrogen removal, and the maximum NH4+-N removal efficiency of 96.69% was achieved in TFCW-Z at HLR of 3 m·d−1 with a flooding and drying cycle of 2 h. Zeolite addition changed the microbial community structure and the abundance of nitrification genes. Comammox Nitrospira was the only enriched strain accounting for NH4+-N removal in TFCW-G, while the co-occurrence of comammox Nitrospira and the canonical and potential ammonia-oxidizing bacteria were identified in TFCW-Z. Summarily, high performance, together with low footprint and low maintenance cost, are characteristics that make the TFCW-Z a promising and competitive alternative.
- Published
- 2020
4. First Report of Botryosphaeria dothidea Causing Gray Mold on Tartary Buckwheat in Southwest China
- Author
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Xin Yao, Ruan Jingjun, Anjing Gao, Yan Peng, Wenfeng Weng, Kaixuan Zhang, Jun Yan, Jianping Cheng, and Yong Tang
- Subjects
Canker ,biology ,Fagopyrum tataricum ,Spots ,Botryosphaeria dothidea ,Plant Science ,medicine.disease ,biology.organism_classification ,Polygonaceae ,Conidium ,Horticulture ,medicine ,Potato dextrose agar ,Agronomy and Crop Science ,Botryosphaeria - Abstract
Tartary buckwheat (Fagopyrum tataricum, Polygonaceae) is an annual plant originating in Southwest China. It has a short growth cycle, barren soil tolerance, and strong stress resistance (Zhang et al. 2021). Because of its high content of proteins, starch, trace elements, phenols, and dietary fiber, Tartary buckwheat is beneficial to the human body and hence has received widespread attention (Joshi et al. 2019; Dc ja, B, et al. 2020). In the period from September to November 2020, a diseased plant infected with gray mold was found among M2 generation plants treated using ethyl methanesulfonate (EMS) in a location with potted Tartary buckwheat plants in Huaxi District, Guiyang City, Guizhou Province, China. The diseased plant started to show symptoms during the initial flowering stage; water-soaked spots appeared at first, that the spots increased in size and turned into light brown patches, with the leaf edges scorched brown. In severe cases, the leaves turned yellow, the diseased spots became dry, and finally the leaves necrotic (Figure 1A). Among the leaves that showed disease symptoms, severely susceptible leaves were selected; a piece of tissue (2×2 mm) was removed at the junction of the diseased and healthy tissues. The tissue was then soaked in 75% ethanol for 2 to 3 s, transferred to 1% sodium hypochlorite solution and soaked for 3 min, rinsed three times with sterile water, and placed on sterilized filter paper to dry. Sterile tweezers were used to transfer the tissue blocks to Potato Dextrose Agar medium (Bio-Rad Ltd. Com, USA) containing a Streptomyces-Penicillium mixture (100 μg/mL), and they were incubated on this medium for 7 to 10 days at 25°C and 70% humidity under 16 h light and 8 h dark conditions. The colonies were white at the early stages, with developed aerial hyphae; subsequently, they gradually turned gray-green (Figure 1B). In the later stages, the back of the colony was black and piles of conidia could be seen (Figure 1C). The conidia are scattered, which were colorless and transparent, fusiform or fusiform, with a size of 8.02-11.13 μm×2.06-3.22 μm (average=9.51 μm×2.69 μm, n=50) (Figure 1D). Based on their morphological characteristics, These cultural and morphological characteristics were consistent with the descriptions of as B. dothidea (Fan et al. 2021). The ITS1/ITS4 (Mills et al. 1992), Bt-2a/Bt-2b primers (Glass and Donaldson 1995), and EF1-728F/EF1-986R (Slippers et al. 2004) were amplified and sequenced to analyze the ITS region, β-tubulin genes translation elongation factor 1-α (TEF1-α), and translation elongation factor 1-α (TEF1-α), respectively. According to BLAST search in GenBank, the sequences of ITS (MZ326853), TUB2 (MZ399162) and TEF1-α (MZ399163) had 99.40%, 100% and 100% similarity to sequences NR111146.1, AY236927.1, and AY236898.1 of B. dothidea ex-type strain CMW8000, respectively. The three nucleotide sequences were concatenated together, and MEGA-X (with the neighbor-joining method) with 1,000 bootstraps was used to construct a phylogenetic tree. The results showed that our isolate was closely related to B. dothidea (Figure 2). Healthy Tartary buckwheat from the M2 generation was used for the pathogenicity test. Disinfect with 75% alcohol and 1×105 mL-1 of spore suspension was sprayed on the leaves. Each treatment included three plants, and it was repeated three times with sterile water as control. The treatments were kept in a houseat25°C for 24 h, then transferred it to the natural environment of 22℃ to 28℃,and sterile water was sprayed every morning and evening to keep the leaves moist. After 10 days, the symptoms seen in the field appeared on the treated plants (Figure 1E), but the control plants did not show any symptoms (Figure 1F). The diseased parts of the leaves were isolated and cultured again, and the isolates were consistent with the original inoculum. Thus, the study conformed to Koch's postulates. B. dothidea is a fungus with no host preference in the genus Botryosphaeria (Botryosphaeriaceae, Botryosphaeriales). It can cause canker, leaf spots, trunk diseases, fruit rot and die-back of many important wood plants all over the world (Marsberg et al.2017). Recently, it was reported that B. dothidea caused soybean canker in China (Chen et al.2021), but there have been no reports of B. dothidea causing Tartary buckwheat gray mold. To the best of our knowledge, this is the first report of B. dothidea causing gray mold on Tartary buckwheat. This finding will provide a basis for the prevention and treatment of Tartary buckwheat gray mold.
- Published
- 2022
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