Search

The goal of this document is to enable users to be able to obtain NMR–metabolomics data on the 800 MHz NMR instrument at the UiO NMR Center. Interpretation of spectra is not (yet) covered. Statistical treatment of the data is also not covered (yet). A database at Ohio State University, which can help you with identification of individual molecules in the metabolomics samples, is mentioned in this document, but the use is not (yet) covered. In order to understand the content of this document the reader need to know how to perform standard nmr-experiments on Bruker nmr- spectrometers.

Documentation of 800 MHz solvent suppression techniques.

Protocol guidelines for running SHMBC and SHSQC experiments on a Bruker AVIIIHD 800 MHz spectrometer.

Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7-10, 7-10, were isolated from shellfish and their structures, previously proposed on the basis of only LC-MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4-6, 4-6, and 7-10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC-MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a time- and concentration-dependent manner; however, there were distinct differences in their EC50 values, with the potencies for each analogue being: AZA6 > AZA8 > AZA1 > AZA4 approximate to AZA9 > AZA5 approximate to AZA10. This data contributes to the understanding of the structure-activity relationships of AZAs.

M20 M21

This paper reports that the abundances of endogenous cardiolipin and phosphatidylethanolamine halve during elongation of the Gram-positive bacterium Listeria innocua. The lyotropic phase behaviour of model lipid systems that describe these modulations in lipid composition indicate that the average stored curvature elastic stress of the membrane is reduced on elongation of the cell, while the fluidity appears to be maintained. These findings suggest that phospholipid metabolism is linked to the cell cycle and that changes in membrane composition can facilitate passage to the succeding stage of the cell cycle. This therefore suggests a means by which bacteria can manage the physical properties of their membranes through the cell cycle.